KR102087106B1 - Biological fertilizer composition and the manufacturing method of biological fertilizer composition for improvement of rhizosphere environment of roadside tree - Google Patents

Abstract

When supplying the bio-fertilizer composition for the roadside root zone environment improvement comprising the soil enzyme mixture and the useful microorganism according to the present invention to the roadside root zone, it is possible to sufficiently supply plant nutrients necessary for the roadside growth, and improve the physical and chemical properties of the root zone soil. It is possible to obtain a tree tree ground and root growth promoting effect.

Description

BIOLOGICAL FERTILIZER COMPOSITION AND THE MANUFACTURING METHOD OF BIOLOGICAL FERTILIZER COMPOSITION FOR IMPROVEMENT OF RHIZOSPHERE ENVIRONMENT OF ROADSIDE TREE}

The present invention relates to a biofertilizer composition for improving roadside root zone environment and a method of manufacturing the same.

Garosu tree improves the aesthetics of the street and provides fresh shades to the citizens by providing pleasant shades in summer, as well as functioning as temperature control, urban micro-climate control, and the connecting axis of the urban green area by absorbing solar radiation. .

Most urban roadside trees are planted near the boundary stone of the L-shaped dike of the sidewalk where the sidewalk blocks or ascons are constructed, and the sidewalks constructed with the sidewalk blocks or the ascones are impermeable soils, soil nutrients and soils. Maintain a poorly ventilated root zone environment.

Since the physical, chemical, phytonutrient and microbiological characteristics of the rhizospheres of trees have not been improved, the huge budget and manpower was wasted due to poor growth, deterioration of water washing, and death. The problem continues to arise.

In normal soil, organic and mineral plant nutrients are mineralized by soil enzymes secreted by soil microorganisms in the soil root zone, and then moved to the soil root zone, and then sequentially through the root wall to cell wall, cell membrane, cytoplasm and phloem. Will be used for metabolism in the plant. However, under poor root zone environment, organic and inorganic plant nutrients treated in soil are decomposed and used for plants, which impose various limitations.

If the nutrients necessary for the growth of the microorganisms are insufficient and the breathability is insufficient, the growth of the microorganisms becomes difficult, and if the population of the microorganisms is insufficient, the degradation of the functions of the soil microorganisms as well as the secretion of soil enzymes are reduced. In this case, the mineralization of the organic and inorganic plant nutrients mentioned above is not normally performed, and it continues to accumulate in the soil, making it difficult to supply plant nutrients and to improve the physical and chemical properties of the rhizosphere soil at the time required for tree tree growth. And death will occur continuously and repeatedly.

Solubilization and circulation of plant nutrients in the soil root zone is mainly caused by soil enzymes secreted by soil microorganisms. However, the tree-planted environment mentioned above is adjacent to the impermeable layer in which the sidewalk block or ascon is constructed, and the soil microbial activity decreases due to soil breathability and lack of plant nutrients, and the production of soil enzymes is slow or produced in the process. This is rarely done. If soil enzymes are not produced or lacked, even if there is a large amount of plant nutrients in the soil, it will be converted into a structure that cannot supply the plant nutrients directly needed.

In conclusion, it is necessary to treat organic and inorganic components related to the improvement of root zone environment in order to grow and grow normal trees on the roadside planting base with poor root zone environment. Therefore, there is a need for a soil enzyme capable of promoting the solubilization of plant nutrients in the soil root zone and containing an appropriate amount of soluble inorganic components that can be directly used by roadside trees. Furthermore, there is a need for a root zone environment improving agent capable of sufficiently supplying functional soil microorganisms that decompose and circulate organic and inorganic compounds in the soil root zone, energy sources necessary for root development and activation of soil microorganisms.

In order to solve this problem, a number of patents exist to protect the growth of trees and to enhance physiological activity. Republic of Korea Patent No. 10-0976445 provides a tree physiologically active nutritional composition comprising a mixture of endogenous microorganisms and salt-tolerant plant extracts, Republic of Korea Patent No. 2001-0007826 is a soil nutrient composition comprising a growth regulator Provide a way to nourish the tree, such as by irrigation. Republic of Korea Patent Publication No. 2001-0067857 and Republic of Korea Patent Publication No. 10-2006-0033756 provides a tree nutrient for fermenting a mixture containing charcoal and the like.

Furthermore, Republic of Korea Patent Publication No. 10-2012-0099873 provides a plant growth development fertilizer containing wood vinegar, fermentation by-products, etc., and many patents including Korean Patent Registration No. 10-1085836 to fertilizers using soil microorganisms It provides an invention related to (Republic of Korea Patent No. 10-1017576, Republic of Korea Patent No. 10-0573477, Republic of Korea Patent No. 10-1043982 and Republic of Korea Patent No. 10-0462953, etc.).

However, if the fertilizer is supplied as a single substance instead of a combination of substances such as soil enzymes or microorganisms, the effect may not be as expected. Therefore, as a biological fertilizer composition containing all of the above components, it does not take a long time to manufacture, and comprehensive fertilization treatment in the roadside root zone environment without the need for direct administration to each plant, thereby providing excellent fertilizer for effectively improving the roadside root zone environment. A composition is required.

One aspect of the present invention is to provide an environmental composition for improving roadside root zone.

Accordingly, another aspect of the present invention is to provide a method for producing a biofertilizer composition to improve the roadside root zone environment.

According to an aspect of the invention the first solution comprising a complex fertilizer containing at least one fertilizer component and plant essential trace elements selected from the group consisting of nitrogen fertilizer, phosphate fertilizer and califer fertilizer; And a soil enzyme mixture comprising at least one soil enzyme; At least one soil root useful microorganism; Seaweed extract; And it provides a bio-fertilizer composition for improving the roadside root area environment containing humic acid.

According to another aspect of the present invention, to prepare a first solution comprising a complex fertilizer containing at least one fertilizer component selected from the group consisting of nitrogen fertilizer, phosphate fertilizer and califer fertilizer and at least one of plant essential trace elements step; A soil enzyme mixture comprising at least one soil enzyme; At least one soil rhizosphere microorganism; Seaweed extract; And preparing a second solution containing humic acid; And it comprises a step of mixing the first solution and the second solution, there is provided a method for producing a bio-fertilizer for street root environment improvement.

According to the biofertilizer composition of the present invention, the mineralization of organic and inorganic plant nutrients in the rhizome root area is normally performed, and the necessary nutrients are supplied to the microorganisms in the soil, thereby improving the function of the soil microorganisms, thereby inducing smooth secretion of soil enzymes and The supply of plant nutrients necessary for growth and the physical and chemical properties of rhizosphere soil are improved to achieve the effect of tree planting and root growth.

1 is the root of the maple tree before the treatment of the biosphere fertilizer for the improvement of the root zone, the control group (10 months without the fertilizer) and the biofertilizer treatment group (10 months after the treatment of the biofertilizer according to the present invention). It is a graph showing the change in diameter.
Figure 2 is the root of the maple tree of the control group (10 months after the fertilizer treatment) and the biofertilizer treatment group (10 months after the treatment of the biofertilizer according to the present invention), before the treatment of the biosphere fertilizer for rhizosphere environmental improvement It is a photograph showing the state of growth.
Figure 3 is a graph showing the change in the dry weight of maple roots of the control group (10 months without fertilizer treatment) and the biofertilizer treatment group (10 months after the treatment of the biological fertilizer according to the present invention).
Figure 4 is a graph showing the change in the chlorophyll content of maple leaves of the control group (10 months without fertilizer treatment) and the biofertilizer treatment group (10 months after treatment with the biological fertilizer according to the present invention).

According to the present invention, the fertilizer for improving the roadside root area environment, which induces the smooth secretion of soil enzymes and improves the physical and chemical properties of the plant nutrients necessary for tree growing and the physical and chemical properties of the root zone soil, thereby promoting the growth of the roadside and root growth. A composition is provided.

More specifically, the composition for improving the roadside root zone environment of the present invention comprises a solution comprising a fertilizer component and a complex fertilizer containing a plant essential microelement, a soil enzyme mixture, a soil rooting zone useful microorganism, a seaweed extract and a solution containing a corrosive extract It is to include.

The fertilizer component used in the present invention is at least one fertilizer component selected from the group consisting of nitrogen fertilizers, phosphate fertilizers and califer fertilizers. For example, at least one component selected from the group consisting of nitrogen, phosphoric acid and potassium contained in the fertilizer can be used.

On the other hand, the plant essential trace elements may be used at least one selected from the group consisting of calcium, magnesium, sulfur, iron, manganese, copper, zinc, boron and molybdenum.

At this time, the fertilizer components and plant essential trace elements may be used in a mixture of two or more kinds, the fertilizer components and plant essential trace elements are mixed to form a complex fertilizer, and the obtained complex fertilizer is referred to as a first solution.

In the preparation of the biofertilizer composition of the present invention, the first solution containing the composite fertilizer is based on the total weight of the first solution of the fertilizer component 5 to 15% by weight And Plant essential trace elements may include 1 to 5% by weight and the balance of water.

When the fertilizer component is less than 5% by weight, the fertilizer component necessary for tree growing is not preferable in that it cannot be sufficiently supplied to the root tree roots. When the fertilizer component is more than 15% by weight, the fertilizer component that is excessively supplied is not evenly spread in the root tree roots. It is not preferable in that it can interfere with root growth due to side effects such as stagnation and root rot.

The compound fertilizer can be prepared as a first solution by dissolving the fertilizer components and plant essential trace elements in water, the pH of the first solution is preferably adjusted to 6.5 to 8. If the pH is less than 6.5, it is not preferable in that it may lead to a decrease in solubility of nitrogen, phosphoric acid and potassium as fertilizer components. If the pH is higher than 8, calcium, magnesium, sulfur, iron, manganese, It is undesirable in that the solubility of copper, zinc, boron and molybdenum can be reduced.

The soil enzyme mixture may be one or more selected from the group consisting of amylase, urease, phosphatase, protease, cellulose and lipase.

The soil root microorganisms include Bacillus sp., Azotobactor sp. And Streptomyces sp., Pseudomonas sp., Aeromonas sp. One or more selected from the group consisting of Saccharomyces sp., Lactobacillus sp. And Penicillium sp. May be used.

The algae to be extracted in the algae extract may be one or more seaweeds selected from the group consisting of green algae, brown algae and red algae.

 The humic acid may be extracted from the corrosive raw material, and the corrosive raw material may be obtained using at least one selected from the group consisting of foliar, peat and peat.

The obtained soil enzyme mixture extract, soil root zone microbial culture solution, seaweed extract and humic acid can be combined to prepare a biological fertilizer as a mixture.

The bio fertilizer mixture is obtained by, for example, 5 to 15% by weight of the soil enzyme mixture extract, 5 to 15% by weight soil root microbial culture, 5 to 10% by weight seaweed extract and 5 to 10% by weight humic extract Contains bio fertilizers. If necessary, the ratio of any one of the above compositions can be increased or decreased, and the mixing ratio is not particularly limited.

The biological fertilizer composition for improving roadside root area environment of the present invention may mix 0.5 parts by weight to 1.5 parts by weight of a second solution per 1 part by weight of the first solution. Preferably, the biofertilizer composition may be obtained by mixing the first solution and the second solution in a 1: 1 weight part.

When the content of the second solution of the biofertilizer composition is less than 0.5 parts by weight, the inorganic components included in the fertilizer component of the first solution may reduce the activity of the microorganisms and cause the growth of the individual, and exceed 1.5 parts by weight. If the nutrients contained in the biofertilizer composition may not be sufficiently supplied to the root tree roots, the microbial activity may be reduced and may not be suitable as a biofertilizer composition for growth of the root tree roots.

When fertilizing the biological fertilizer mixed in the above ratios, the mineralization of organic and inorganic plant nutrients in the root zone of the roadside tree may be normally performed. As a result, the nutrients necessary for the microorganisms in the soil are sufficiently supplied, thereby obtaining a biofertilizer composition for improving the roadside root area environment, which may improve the function of the soil microorganisms.

According to another aspect of the present invention, a fertilizer component containing at least one of nitrogen, phosphoric acid and potassium selected from the group consisting of nitrogen fertilizer, phosphate fertilizer and califer fertilizer and at least one or more of plant essential trace elements Preparing a first solution, preparing a second solution comprising at least one soil enzyme mixture comprising at least one soil enzyme, at least one soil root microorganism, an algae extract, and a humic acid; and Provided is a method of preparing a bio-fertilizer composition for improving roadside root zone environment, comprising mixing a second solution.

The preparing of the first solution may include calcium, magnesium, sulfur, iron, manganese, copper, zinc and boron as at least one fertilizer component selected from the group consisting of nitrogen fertilizer, phosphate fertilizer and califer fertilizer as plant fertilizer and plant essential trace elements. At least one element selected from the group consisting of molybdenum may be mixed with deionized water. Therefore, the first solution, which is a complex fertilizer, can be obtained by the mixing.

The soil enzyme mixture may be obtained by filtering a mixture of phosphate buffer solution mixed with soil containing organic matter, and then adding ammonium sulfate to the filtrate.

The soil may be a soil containing organic matter, and preferably, the organic matter content may be 3 wt% to 3.5 wt% based on 100 wt% of the soil. If the organic content is less than 3% by weight, the number and species diversity of microorganisms in the soil may not be secured, and thus the amount of soil enzyme may be insufficient. If it exceeds 3.5% by weight, it is not preferable in that the organic matter present in the soil is excessively supplied so that the physical and chemical properties of the soil can be kept low.

Soil enzyme mixture can be prepared using the obtained organic matter. For example, the obtained filtrate may be obtained by mixing 1-3 ml of 0.1 M phosphate buffer solution per 1 g of the obtained soil and centrifuging at room temperature. 50 to 60 g of ammonium sulfate is mixed per 100 ml of the filtrate obtained above. The resulting precipitate can be centrifuged again to obtain a soil enzyme mixture.

The soil enzyme mixture thus obtained may have multiple soil enzymes. For example, amylase enzymes that break down carbohydrate molecules, protease enzymes that break down protein molecules, lipases that break down fat molecules, and ureases that break down urea into carbon dioxide and ammonia. ), A cellulase enzyme (cellulase) that breaks down cellulose, phosphate esters and phosphatase enzymes that catalyze the hydrolysis of polyphosphoric acid may be included in the mixture.

The seaweed extract may be obtained by mixing an extraction solvent with seaweed of the powder formulation, which is dried and ground to a water content of 20% or less. The extraction solvent may be a solvent capable of obtaining a water-soluble useful ingredient together with a fat-soluble useful ingredient contained in seaweed, and preferably includes an alcohol having water-soluble and fat-soluble properties, but is not particularly limited.

The algae which is a raw material of the algae extract may be one or more seaweeds selected from the group consisting of green algae, brown algae and red algae.

In order to prepare the seaweed extract, for example, the step of washing the wakame with water so that the salt concentration of the wakame 0.4 to 0.6% may be further performed. After washing the washed wakame seaweed may be pulverized so that the average particle size of the powder of the dried wakame particles to 1 to 2mm to obtain a seaweed of the powder formulation. At this time, the grinding method is not particularly limited.

The specific extraction process for extracting the seaweed extract from the obtained seaweed is not particularly limited. For example, after mixing about 1 L of ethanol 60 to 80% as an extraction solvent per 1 kg of the powdered algae, the algae extract may be obtained using a Soxhlet extractor. The obtained seaweed extract is preferably sealed and refrigerated.

On the other hand, the humic acid used in the present invention may be used as at least one selected from the group consisting of side loam, peat and peat as a corrosive raw material.

The mixture obtained by mixing the strong alkaline solution with the corrosive raw material may be solid-liquid separated to obtain a primary precipitate, and an acidic mixture may be obtained by adding hydrochloric acid to the solution from which the precipitate is removed. Thereafter, the solution from which the acidic mixture is removed may be solid-liquid separated to obtain a secondary precipitate.

For example, a strong alkali solution having a pH of 12 or more may be prepared by dissolving 400 g of 100% sodium hydroxide (NaOH), which is a strong alkali solution, in 1 L of water. After leaving the mixture in which the sodium hydroxide and the corrosive raw material were mixed at a ratio of 60 to 80 parts by weight: 20 to 40 parts by weight, the precipitated precipitate was separated and the separated precipitate was dried. The dry precipitate may be milled to obtain a primary humic acid powder. Furthermore, the precipitate is separated and the remaining solution is acidified to pH 1-2 by adding hydrochloric acid to the refiltered filtrate solution. In the same manner as above it can be obtained after the solid-liquid separation and drying to obtain a secondary humic acid powder. The powdering of the primary and secondary humic acids can be carried out individually or together, which can be used individually or in combination.

The second solution may be obtained by mixing the obtained soil enzyme mixture, soil root microbial culture, algae extract and humic acid.

More specifically, the second solution is preferably obtained by combining 5 to 15% by weight soil enzyme mixture, 5 to 15% by weight soil root microorganism, 5 to 10% by weight seaweed extract and 5 to 10% by weight humic acid. have. However, if necessary, the mixing ratio may be increased or decreased, so that the mixing ratio is not particularly limited.

The pH of the obtained microbial strain is preferably 8 to 9. The strain may be in the spore state regardless of the temperature in the alkaline region of pH 8 to 9 to ensure the number of microorganisms.

The biofertilizer composition for improving the roadside root area environment of the present invention may be obtained by mixing the first solution and the second solution in an amount of 1: 0.5 to 1.5 parts by weight, and most preferably the biofertilizer composition may include the first solution and the second solution. The solution can be obtained by mixing at 1: 1 parts by weight. When the content of the second solution of the biofertilizer composition is less than 0.5 parts by weight, the inorganic components included in the fertilizer component of the first solution may reduce the activity of the microorganisms and cause the growth of the individual, and exceed 1.5 parts by weight. If the nutrients contained in the biofertilizer composition may not be sufficiently supplied to the root tree roots, the microbial activity may be reduced and may not be suitable as a biofertilizer composition for growth of the root tree roots.

When the biological fertilizer composition thus prepared is used as soil irrigation in the rhizosphere, it is possible to improve the physicochemical, phytonutrient and microbiological characteristics, and increase the useful microbial population and soil enzyme activity in the soil. Promote the growth of roots can be obtained.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to easily understand the present invention, the present invention is not limited to the following examples.

Example

1. Preparation of biofertilizer composition for improving the roadside root zone environment

(1) Acquisition of Soil Enzyme Mixture

For the preparation of soil enzyme mixtures, field soils containing more than 3% by weight of organic matter were obtained from agricultural land in Gongdeok-myeon, Gimdeok-si, Jeollabuk-do. A mixture of 2 ml of 0.1 M phosphate buffer solution (pH 7.0) was mixed with 1 g of the soil at room temperature for 30 minutes, followed by centrifugation at 10,000 rpm for 10 minutes, and the mixture was filtered. 56 g of ammonium sulfate was added to 100 ml of the filtrate at 4 ° C and stirred with a stirrer. After about 12 hours, the precipitate was centrifuged at 10,000 rpm for 15 minutes (Soil Microbiology, 2002).

Amylase enzymes that degrade carbohydrate molecules, protease enzymes that break down protein molecules, and lipases that break down fat molecules from sediments obtained by centrifugation. Enzymes (lipases), ureases that break down urea into carbon dioxide and ammonia, cellulase enzymes that break down cellulose, phosphate esters and phosphatases that catalyze the hydrolysis of polyphosphates Soil enzyme mixture was obtained.

(2) Acquisition of Soil Root Microorganisms

In order to obtain soil root microorganisms, the same soil as used in the above (1) was used for the selection of biological strains.

Photosynthetic bacteria of Aeromonas sp. Were isolated from the soil using Rhodopseudomonas blastica Medium, and the genus Saccharomyces using Dextrose Yeast Extract Peptone. (Saccharomyces sp.) Yeasts were isolated. Lactic acid bacteria of the genus Lactobacillus sp. Were isolated using Lactic Bacteria Medium, and penicillium was isolated using Czapek agar medium. Bacillus was isolated using NutrientAgar and Azotobacter was isolated using Azotobacter chroococcum Agar (Soil Microbiology, 2002). After the identification of the microorganisms collected, liquid culture was performed in accordance with the Soil Microbiology Test Method (2002) to secure the cells.

The obtained microbial cells were maintained at 30 ° C. under anaerobic conditions after inoculation in a culture medium (1% molasses, 0.5% rice, Water 1L), and cultured for 7 days, and the number of microbial cells in the range of 1 × 10 ^{7 to} 1 × 10 ^8. Obtained.

(3) Acquisition of Seaweed Extract

To prepare seaweed extract, seaweed ( Undaria pinnatifida ) was obtained from aquaculture farm in Wando-eup, Wando-gun, Jeollanam-do, and after 6 hours of soaking in clean water, it was washed with clean water again 3 ~ 5 times to remove salts remaining on the surface. % Was obtained. Desalted and washed seaweed was dried in a dryer (60 ° C., 6 hours) to 20% and then ground into a powder formulation of 1 to 2 mm size particles. The mixed solution containing 1 kg of seaweed and 1% of 70% ethanol of the powder formulation was subjected to an extraction operation using a Soxhlet extractor at a temperature of 95 ° C. for 2 hours to obtain a wakame extract. The obtained wakame extract was sealed and refrigerated at 4 ° C.

(4) acquisition of humic acid

In order to obtain humic acid, commercially available peat was used as a corrosive raw material used in the present invention. 400 g of 100% sodium hydroxide (NaOH) was dissolved in 1 L of water to prepare a strong alkali solution having a pH of 12 or more. The strong alkali solution and peat were mixed at 70% by weight: 30% by weight and stirred for 1 hour at 100 rpm stirring speed.

After the stirring, the precipitate obtained by standing for 24 hours or more was solid-separated at 3,000 rpm in a centrifuge to dry the precipitate obtained (60 ℃, 5 hours). The dried precipitate was ground to a size of 360 mesh or less in a mill to obtain a primary powder humic acid. Furthermore, the precipitate was separated and the remaining solution was filtered through a filter of 360 mesh or more, and hydrochloric acid (35%) was added to the filtrate to have a pH of 1 to 2. The precipitate precipitated by solid-liquid separation of the filtrate solution to which the hydrochloric acid was added was dried in the same manner to obtain a secondary powder humic acid. The obtained primary powder humic acid and the secondary powder humic acid were used by mixing at a ratio of 60% by weight: 40% by weight.

 (5) acquisition of compound fertilizer

To 1 liter of sterilized deionized water, 5 parts by weight of nitrogen (urea fertilizer), 3 parts by weight of phosphoric acid (hyperphosphate fertilizer), and 4 parts by weight of kali (calibrated chloride fertilizer) were added, respectively, in a stirrer (120 rpm). Stir for 30 minutes. 1 part by weight of calcium (quick lime), 1 part by weight of magnesium (magnesium sulfate), 0.5 part by weight of sulfur (water-soluble sulfur), 0.05 part by weight of iron (ferrous chloride), per 100 parts by weight of the deionized water, 0.01 parts by weight of manganese (manganese sulfate), 0.01 parts by weight of copper (copper sulfate), 0.005 parts by weight of zinc (zinc sulfate), 0.001 parts by weight of boron (boric acid) and 0.001 parts by weight of molybdenum (molybdate) It mixed and obtained the pH weakly acidic composite fertilizer aqueous solution. NaOH (4%) was added to the obtained aqueous fertilizer solution to adjust the pH to 8.

The solution containing the composite fertilizer thus obtained is referred to as 'first solution'.

(6) Acquisition of biofertilizer composition for improving roadside root zone environment

10% by weight of the soil enzyme mixture obtained in (1), 10% by weight of soil root microbial culture medium, 10% by weight of seaweed extract, 10% by weight of humic acid extract and the remainder of water and mixed for 20 minutes in a stirrer (120rpm) A second solution was obtained.

The pH of the second solution prepared as described above was 8, so that the microbial strain was present in the spores regardless of the temperature in the alkaline region of about pH 8 was able to guarantee the number of microorganisms.

After mixing the first solution and the second solution in a 1: 1 ratio, the mixture was mixed for 10 minutes in a stirrer (120 rpm) to obtain a bio-fertilizer composition for improving the roadside root area environment of the present invention.

2. Confirmation of the treatment effect of biofertilizer for improving roadside root zone environment

The efficacy test of the biofertilizer prepared in 1. was carried out on 10 weeks of 3 year old maple tree.

The height of the maple tree under investigation was 1.7 ± 0.7m and the root diameter of the maple was 1.9 ± 0.8cm. After drilling the core to the root zone of the maple tree (30 cm from the surface) with a soil core collector (10 cm in diameter), total 500 ml of the fertilizer obtained in 1. Once in 10 months Soil irrigation was performed.

10 months after the control process (no treatment) and the biological fertilizer according to the present invention obtained in the above 1. subject to 10 months after maintaining the same environmental conditions such as light, humidity, etc. Changes in maple root diameter, root dry weight, root growth status, chlorophyll content, soil microbial community density and enzyme activity in soil were investigated.

The root diameter was measured by the diameter of the tree touching the surface of the maple tree.

In order to measure the root biomass, the roots of the basement were cut and washed about 5 times with tap water to remove soil or foreign matter and then dried in a dry oven at 60 ° C. for 12 hours. The dried roots were weighed using an electronic balance.

Chlorophyll content of trees was measured using a chlorophyll meter (SPAD-502: Minolta, Japan). Soil microbial community density was investigated by dilution plate method for aerobic bacteria, filamentous fungi and actinomycetes in soil topsoil (0-5cm). Enzyme activity in the soil was investigated in the same way for phosphatase, protease, and urease among enzymes (soil microbiology, 2002).

As a result of measuring the growth change of the roadside tree (foliage) according to the treatment of bio-fertilizer composition for roadside root zone environment improvement prepared as described above was obtained.

The change in root diameter is measured and shown in FIG. 1. The root diameter of the maple tree before treatment was 1.9 ± 0.8cm, and after 10 months, 2.3 ± 1.3cm in the control (no treatment) and 3.5 ± 1.1cm in the biofertilizer treatment showed a root diameter of the biofertilizer treatment compared to the control. 27% increase was confirmed.

The dry weight change of the maple root was measured and shown in FIG. 2. The control (no treatment) was 50.2 ± 4.8g and the biofertilizer treatment was 64.6 ± 7.3g. Therefore, it was confirmed that the source diameter of the biofertilizer treatment group increased by about 22% compared to the control.

As a result of visual inspection of the growth of maple roots, as shown in FIG. 3, not only was the generation of root hairs more active in the biofertilizer treatment group than the control group, and it was confirmed that the development of the main root and the root muscle was much superior.

The total chlorophyll content of the maple leaves is shown in FIG. 4. The control group (no treatment) was 22.5 ± 6.1mg / g (fresh weight), and the biofertilizer treatment group was 25.1 ± 5.6mg / g (fresh weight), which showed about 10% increase in the total chlorophyll content of the control group. It was confirmed.

Soil microbial population of the soil root zone was as Table 1 below.

 division Aerobic bacteria Filamentous fungi Actinomycetes cfu / g Control 6.8 x 10 ⁶ ± 0.7 8.1 x 10 ⁵ ± 1.2 2.4 x 10 ⁵ ± 1.1 Biofertilizer Treatment Zone 7.3 x 10 ⁷ ± 1.2 9.2 x 10 ⁵ ± 1.7 6.5 x 10 ⁵ ± 0.5

As shown in Table 1, the number of aerobic bacteria was 6.8 × 10 ⁶ ± 0.7 cfu / g in the control, and 7.3 × 10 ⁷ ± 1.2 cfu / g in the biofertilizer. The number of filamentous fungi was 8.1 × 10 ⁵ ± 1.2 cfu / g in the control and 9.2 × 10 ⁶ ± 1.7 cfu / g in the biofertilizer. Actinomycetes showed 2.4 × 10 ⁶ ± 1.1 cfu / g in the control and 6.5 × 10 ⁶ ± 0.5 cfu / g in the biofertilizer. Therefore, the treatment of the biofertilizer according to the present invention was confirmed to increase the increase in the number of aerobic bacteria.

The activity of the soil enzyme secreted by the microorganisms in the soil is shown in Table 2 below.

division Phosphatase Protease Eurese
(urease) Μg PNP / g / hr mmole leucine / kg / hr mgNH ₄ -N / kg / hr Control 34.5 ± 4.2 31.3 ± 2.8 88.9 ± 5.5 Biofertilizer Treatment Zone 44.1 ± 5.5 39.5 ± 1.9 118.3 ± 11.1

The phosphatase showed 34.5 ± 4.2 μg PNP / g / hr in the control and 44.1 ± 5.5 μg PNP / g / hr in the biofertilizer. The protease showed 31.3 ± 2.8mmole leucine / kg / hr in the control and 39.5 ± 1.9mmole leucine / kg / hr in the biofertilizer. U race (urease) exhibited a _{88.9 ± 5.5mg NH 4 -N / kg} / hr, 118.3 ± 11.1mg NH 4 -N / kg / hr in the biological fertilizer treatment in the control group. Therefore, the biofertilizer treatment according to the present invention was confirmed to improve the activity of the soil enzyme.

As described above, when the bio-fertilizer composition for improving roadside root area environment of the present invention was not treated, the number of useful microorganisms in soil and activity of soil enzymes were increased by 20-30% or more. Therefore, when treating the bio-fertilizer composition for improving the roadside root zone environment according to the present invention, it was confirmed that the roadside ground and root growth are promoted.

Claims (15)

A first solution comprising at least one fertilizer component selected from the group consisting of nitrogen fertilizers, phosphate fertilizers and califer fertilizers, and complex fertilizers containing at least one or more of plant essential trace elements; And
Soil enzyme mixture containing at least one soil enzyme, at least one soil root root useful microorganism, seaweed extract and a humic acid, including a second solution having a pH of 8 to 9, a tree-roast root environment improving organism Fertilizer composition. The method of claim 1,
The fertilizer component is at least one or more selected from the group consisting of nitrogen, phosphoric acid and potassium, bio-fertilizer composition for improving the roadside root zone environment. The method of claim 1,
The plant essential trace element is at least one selected from the group consisting of calcium, magnesium, sulfur, iron, manganese, copper, zinc, boron and molybdenum, bio-fertilizer environment improvement bio-fertilizer composition. The method of claim 1,
The fertilizer component of the first solution is a fertilizer component 5 to 15% by weight, plant essential trace elements 1 to 5% by weight and the balance of water, bio-fertilizer composition for improving the roadside root area environment. The method of claim 1,
The composite fertilizer has a pH of 6.5 to 8, bio-fertilizer composition for improving roadside root zone environment. The method of claim 1,
The soil enzyme mixture is one or more selected from the group consisting of amylase, amylase, urease, phosphatase, protease, cellulose and lipase, and improve the tree root root environment. Biofertilizer composition for. The method of claim 1,
The soil root useful microorganisms include the genus Bacillus sp., Azotobactor sp. And Streptomyces sp., Pseudomonas sp., And Aeromonas sp. , Saccharomyces sp., Saccharomyces sp., Lactobacillus sp. And Penicillium sp. The method of claim 1,
The algae of the algae extract is using a seaweed algae selected from the group consisting of green algae, brown algae and red algae, bio-fertilizer composition for improving the roadside root zone environment. The method of claim 1,
Corrosive raw material of the humic acid is at least one selected from the group consisting of foliar, peat and peat, bio-fertilizer composition for improving the roadside root area environment. The method of claim 1,
The second solution is 5 to 15% by weight soil enzyme mixture;
5-15% soil root zone microorganisms;
5-15% seaweed extract;
5-15% by weight of humic acid; And
Bio-fertilizer composition for improving roadside root area environment containing the remainder of water. The method of claim 1,
The weight part of the second solution per 1 part by weight of the first solution is 0.5 to 1.5, bio-fertilizer composition for improving roadside root area environment. Preparing a first solution comprising at least one fertilizer component selected from the group consisting of nitrogen fertilizers, phosphate fertilizers and califer fertilizers and a complex fertilizer containing at least one or more of plant essential trace elements;
Preparing a second solution comprising a soil enzyme mixture comprising at least one soil enzyme, at least one soil root microorganism, seaweed extract, and humic acid, the pH of which is 8-9; And
Comprising the step of obtaining the biofertilizer composition by mixing the first solution and the second solution, bio-fertilizer manufacturing method for improving roadside root area environment. The method of claim 12,
The seaweed extract,
Drying the seaweed to a water content of 20% or less; And
Extracting by mixing an extraction solvent with dried seaweed;
Will be prepared, including a planting method for improving fertilizer root zone environment. The method of claim 12,
The humic acid is obtained by solid-liquid separation of a mixture of at least one corrosive raw material and a strong alkali solution selected from the group consisting of foliar, peat and peat to obtain a primary precipitate; And
Obtaining a secondary precipitate by the solid-liquid separation of the acid mixture obtained by adding hydrochloric acid to the remaining solution after the solid-liquid separation, a bio-fertilizer for improving the roadside root area environment. The method of claim 12,
The biofertilizer composition comprises 0.5 to 1.5 parts by weight of the second solution per 1 part by weight of the first solution, bio-fertilizer manufacturing method for improving roadside root area environment .

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