CN105315041A - Preparation method and application of calcium polypeptide promoter for improving soil micro-ecological population quantity - Google Patents
Preparation method and application of calcium polypeptide promoter for improving soil micro-ecological population quantity Download PDFInfo
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- CN105315041A CN105315041A CN201510782483.XA CN201510782483A CN105315041A CN 105315041 A CN105315041 A CN 105315041A CN 201510782483 A CN201510782483 A CN 201510782483A CN 105315041 A CN105315041 A CN 105315041A
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Abstract
The invention discloses a preparation method and application of a calcium polypeptide promoter for improving soil micro-ecological population quantity and belongs to the technical field of protein waste treatment and recycling. The preparation method comprises the following steps: adding a protein-containing raw material into a high-temperature reaction kettle; after properly adding calcium oxide or quicklime and water into the reaction kettle, heating up to 121-170 DEG C, keeping the temperature for 0.5-6.5 h, and cooling down to the ordinary temperature to obtain the calcium polypeptide promoter, wherein the protein-containing raw material is food factory sludge, urban life sludge or plant cake meal, and the weight of the added calcium oxide or quicklime accounts for 10-35 percent of that of dehydrated dry sludge or the plant cake meal. The calcium polypeptide promoter can be used for increasing the soil micro-ecological population quantity and quickly promotes microbial growth, and plants planted with the calcium polypeptide promoter is higher in content of chlorophyll than plants planted with ordinary fertilizers, is large in biomass, and contains most soluble starch and other nutritional ingredients in all plants planted with fertilizers.
Description
Technical field
The Treatment and recovery that the invention belongs to albumen waste utilizes technical field, be specifically related to utilize albumen waste prepare quick-acting, without the fertilizer of nitrogen discharge and regulate and control soil micro-ecosystem, more particularly, the present invention relates to a kind of preparation method and the application that improve the calcium polypeptide promotor of soil micro-ecosystem population quantity.
Background technology
Along with the development of modern agriculture, country is also increasing to the demand of fertilizer.How obtaining fertilizer is China's agricultural problem demanding prompt solution.The fertilizer sold on the market, according to different properties and the feature of fertilizer, three major types can be divided into, one is chemical fertilizer, also known as mineral manure, be the fertilizer produced with chemistry or physical method, as ammonium sulfate, bicarbonate of ammonia, urea, potassium sulfate, calcium superphosphate and ground phosphate rock etc., they can directly supply some nutritive element for plant, improve plant biomass; Two is organic fertilizer, also known as farm manure, it contains the various nutritive elements needed for abundant organic matter and plant, through transforming, plant multiple nutrients can be supplied, there is improvement soil, strengthen the effect of soil microbial activities, as people and animals' fecaluria, green manure, heap make compost, cake fertilizer etc., be a kind of complete manure; Three is bio-feritlizers, and also known as bacterial manure or microbial inoculum, being by the formulated biological fertilizer of one or several beneficial microorganisms, culture medium and additive, as nitragin and various biotechnological formulations etc., is a kind of indirect fertilizer.
These three kinds of fertilizer have respective relative merits, although with urea be some chemical fertilizer of representative directly by plant utilization, improve crop yield in the short period of time, and with low cost, nitrogen content is high, is easy to run off, and causes utilization rate of fertilizer low.And urea is manured into soil after hydrolysis becomes volatile salt or bicarbonate of ammonia, can decompose further and release ammonia, ammonia release too much, can cause burning seedling, even injures seed and root system.Use the crumb structure of urea meeting spoiled soil for a long time, change soil physical chemistry character, affect the normal activity of soil microorganisms, cause soil fertility to decline.Though composite fertilizer is containing multiple nutritional components, but the ratio of its various nutritive ingredient is fixed, a kind of fertilizer of usual use is difficult to the demand meeting various plants or same plant different growing stages, and life-time service easily damages the structure of soil, has a negative impact to microbial growth.Fertilizer nutritive ingredient is comprehensive, and fertilizer efficiency is lasting, and can improve soil microbe quantity to a certain extent, improves the soil texture.But also have shortcoming, such as farm manure complicated component, containing a lot of pathogenic micro-organisms, need a series for the treatment of processess such as compost, and fertilizer efficiency is slow, and even the longer time executes competence exertion in soil and well acts on to need two weeks in advance.The fertilizer efficiency of bio-bacterial manure and number of viable, temperature, moisture, pH etc. are closely related, therefore just carve requirement for environmental conditions, and somewhat expensive, promote not easily.
Summary of the invention
In view of mineral manure destructible Soil structure, fertilizer fertilizer efficiency is slow and organic, all there is easy loss in inorganic fertilizer, the shortcoming such as volatile, the present invention proposes and utilize containing albumen waste (rapeseed cake, cottonseed cake, food factories mud or the lower sludge generated in municipal domestic sewage of heavy metal content) through biochemical conversion be the calcium polypeptide organic fertilizer that fertilizer efficiency speed is suitable with urea, available nutrient is converted into microorganism cells constituent by improving soil micro-ecosystem population quantity fast simultaneously, thus realize without fertilizer loss, be equivalent to the effect of biological slow-release fertilizer, specifically, relate to and a kind of preparation method and the application that improve the calcium polypeptide promotor of soil micro-ecosystem population quantity are provided.
In order to realize above-mentioned first object of the present invention, the present inventor is also constantly explored by lot of experiments research, finally obtains following technical scheme:
A kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity, described method will add in pyroreaction still containing protein raw materials, and add a certain amount of calcium oxide or unslaked lime and appropriate water in reactor after, be heated to 121 ~ 170 DEG C, insulation 0.5 ~ 6.5h, after being cooled to normal temperature, obtain described calcium polypeptide promotor, wherein, described is food factories mud, municipal sludge or plant cake containing protein raw materials, and the calcium oxide of described interpolation or the weight of unslaked lime account for 10 ~ 35% of dehydration dewatered sludge or plant cake weight.
Further, containing protein raw materials described in technique scheme is plant cake, and Heating temperature is 121 ~ 130 DEG C.
Further, containing protein raw materials described in technique scheme is food factories mud or municipal sludge, and Heating temperature is 150 ~ 165 DEG C.
Further, heating described in technique scheme adopts in reactor, passes into the direct-fired mode of water vapor, and the pressure of described water vapor is 0.6 ~ 0.9Mpa.
Further, the weight of the water added described in technique scheme is described containing 100% ~ 400% of protein raw materials weight.
Further, the calcium oxide added described in technique scheme or the weight of unslaked lime are preferably 20% of described plant cake weight.
Further, the calcium oxide added described in technique scheme or the weight of unslaked lime are preferably 32% of dehydration dewatered sludge weight.
Further, plant cake described in technique scheme is any one or more in rapeseed cake, cottonseed cake, vinasse.
Further, in the plant cake raw material described in technique scheme, protein content is 38 ~ 45%, and plant cake raw material pulverizing degree is 1 ~ 2mm.
Further, the water content of the mud of food factories described in technique scheme is 75 ~ 85%, is preferably 80%, and the dewatered sludge protein content that wherein dewaters is preferably 55%.
The heavy metal content of municipal sludge described in technique scheme is suitable with food factories sludge heavy-metal content.
Another object of the present invention is to the application that a kind of above-mentioned obtained calcium polypeptide promotor is provided.
For improving a fertilizer for soil micro-ecosystem population quantity, described fertilizer is the calcium polypeptide promotor utilizing aforesaid method of the present invention to prepare.
Prepared by the present invention, calcium polypeptide promotor according to every square metre is: calcium polypeptide promotor 200ml(calcic polypeptide 20 ~ 25%) and the water 400ml(rainy day can not add water) directly mixing be applied in the soil of non-planting plants, can directly sow or transplant seedling after 5 days, now a large amount of microorganism grows, soil micro-ecosystem population quantity significantly improves, and can produce High quality agricultural products; Be implanted with crop if planted, can directly mixed calcium polypeptide (according to the different suitably decrement of crop) promotor watered in soil as topdressing, the double effects improving soil micro-ecosystem population quantity, promote crop growth can be reached.Based on this, the invention provides a kind of application scheme improving the calcium polypeptide promotor of soil micro-ecosystem population quantity.
Compared with prior art, the calcium polypeptide prepared by the present invention, can promote the growth of soil microorganisms rapidly, improve soil micro-ecosystem population quantity, and can enrich and comprehensively nutrition for crop growth provides, and effect is lasting, preparation is simple, both can make base manure and also can topdress.Be described as follows:
(1) calcium polypeptide is compared with traditional fertilizer, large protein molecule is relatively less, and the small molecular content such as peptide class, amino acid are high, can fast by antimicrobial absorbent after therefore entering soil, promote microbial growth, to reach the object improving soil micro-ecosystem population quantity;
(2) calcium polypeptide energy accelerate growth of microorganism fast, through the monitoring of 2 years, Soil Microorganism population structure is far away higher than all fertilizer (comprising bacterium, actinomycetes, fungi), a large amount of microorganisms can produce multiple enzyme, accelerate the nitrogen element of soil and the conversion of other element, these elements are utilized by farm crop;
(3) calcium polypeptide can promote quick growth of plant, and only need 5 ~ 7 days plants variable green (crow green, urea be dark green) when to be applied in soil, its speed is equal to urea;
(4) calcium polypeptide is complete water-soluble active substance, is applied in soil, and its nitrogen content is stablized, and without running off and migration, being transformed very soon, play biological nitrogen fixation because calcium polypeptide institute is nitrogenous by antimicrobial absorbent;
(5) calcium polypeptide can the cash crop of production high-quality, and early-stage Study shows, institute's its chlorophyll of kind of plant is all higher than all fertilizer institutes planting plants, and biomass is large, and the nutritive ingredients such as Zulkovsky starch contained by plant are the highest in all fertilizer.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in detail.
Embodiment 1
The present embodiment is that calcium polypeptide promotor of improving soil micro-ecosystem population quantity of the present invention prepared by raw material with rapeseed cake.
At 10m
3mixer in add the rapeseed cake 2800kg pulverized, with the unslaked lime of 5600kg water-solubleization 560kg, prepare lime milk solution, by this lime solution input be added with in the mixer of rapeseed cake, stir, input 10m with pump
3reactor in, pass into 0.6 ~ 0.9Mpa and be steam heated to 125 DEG C, insulation 2h, beaker sampling observation, solution colour is dark brown and the layering of solid substance and liquid portion, namely shows that reaction is complete, is cooled to normal temperature, it is 10.5 that pH test paper measures its pH value, and this solidliquid mixture is described calcium polypeptide promotor.
Embodiment 2
The present embodiment with food factories mud for calcium polypeptide promotor of improving soil micro-ecosystem population quantity of the present invention prepared by raw material.
At 5m
3mixer in add food factories mud 3000kg(moisture 80%, dry product is containing albumen 48%), with the unslaked lime of 360kg water-solubleization 180kg, prepare lime milk solution, the input of this lime solution be added with in the mixer of mud, stirring all with, input 5m with pump
3reactor in, pass into 0.6 ~ 0.9Mpa and be steam heated to 155 DEG C, insulation 4h, open vent valve, stink if any mud illustrates and can not sample observation, insulation need be continued 1 hour, sample with graduated cylinder when odorless, if solution presents muddy state, namely show that the lime being slightly soluble in water is not converted into calcium polypeptide yet, if precipitated very soon, and supernatant liquor is the liquid of thickness, namely show albumen decomposition and inversion be calcium polypeptide, utilize steam overbottom pressure by calcium polypeptide mixture input surge tank to reduce pressure, now utilize 40m
2the board and frame machine of left and right filters, and filtrate is carried out multiple-effect evaporator and concentrates, until 20Be
0(being equivalent to dry-matter 40%w/v), utilizing spectrophotometer method to measure calcium peptide concentration is 25%(w/v) left and right, it is 11.0 that pH test paper measures its pH value, and this concentrated solution is calcium polypeptide promotor.
Application test example 1
Calcium polypeptide promotor obtained for the above embodiment of the present invention 1 is applied to and improves soil micro-ecosystem population quantity.
By 15m
2soil be divided into equal 3 pieces, wherein a piece of land applies the calcium polypeptide accelerator solution of 200g, 400ml(rainy day that simultaneously adds water can not add), the urea of the nitrogen quantity such as one piece of applying and water, the fertilizer of the nitrogen quantity such as another a piece of land applying, and apply appropriate phosphate fertilizer potash fertilizer to three blocks of soils, ensure that the phosphoric in these three blocks of soils is the same with potassium element.Within 10 days, measure the microorganism total amount of soil afterwards.Microorganism measuring method: configure 1000ml extractum carnis substratum respectively, Gause I substratum, Rose Bengal Sodium substratum.Plate is down flat after high-temperature sterilization, for subsequent use after cooling; Get the topsoil on three pieces of ground, namely get 5g wet soil respectively and add 45ml sterilized water, 180r/min vibrates 20min, and carry out gradient dilution successively: bacterium, actinomycetes are diluted to 10 respectively
-5doubly, 10
-6doubly; Fungi is diluted to 10
-4doubly, 10
-5doubly.Each concentration repeats at least 3 times; Bacterium, 28 DEG C, actinomycetes are cultivated 7 days, and fungi 25 DEG C is cultivated 7 days; Finally choose 10
-6for suitable concn counting, and the sum that three is added is compared, microbe population result is as shown in table 1.
As can be seen from the result of table 1, apply the soil of the calcium polypeptide promotor that the present invention obtains, in soil, Tiny ecosystem population quantity is apparently higher than the quantity of microorganism in the soil using common urea, fertilizer, and therefore, the calcium polypeptide promotor that the present invention obtains can improve soil micro-ecosystem population quantity.
Table 1 utilizes the soil topsoil microbe population result of different fertilizer process
| The soil of different fertilizer process | Microbe population (unit: individual/g) |
| The soil of Urea treatment | 1.61×10 7 |
| The soil of fertilizer process | 2.33×10 7 |
| The soil of calcium polypeptide process | 8.97×10 7 |
Application test example 2
Calcium polypeptide promotor the present invention obtained is applied to research plant chlorophyll being improved to effect.
Plantula Brassicae chinensis seed in the soil kind of above-mentioned application test example 1, ensure that every block seed number is on the ground roughly the same, after treating that it sprouts, win small and weak seedling, make its every block Chinese cabbage seedling on the ground the same, apply fertilizer when it grows to about 5cm, wherein a piece of land applies the calcium polypeptide accelerator solution of 100ml, 400ml(rainy day that simultaneously adds water can not add), the urea of the nitrogen quantity such as one piece of applying in other two pieces and water, the fertilizer of the nitrogen quantity such as another a piece of land applying, and apply appropriate phosphate fertilizer potash fertilizer to three blocks of soils, ensure that the phosphoric in these three blocks of soils is the same with potassium element.Within 40 days, measure identical chlorophyll content in leaf blades later.Measuring method: get Fresh leaves and clean, dry, removing petiole, take about 0.5 gram, in test tube, add 25ml, 95% ethanol, do reagent blank, encase with black sack, put into refrigerator and cooled to hide, until leaf is colourless, at A665, survey absorbancy under the light of A649 and A470 tri-kinds of wavelength, and go out chlorophyll content by formulae discovery.Formula is as follows:
Chlorophyll-a concentration (mg/L) Ca=13.95*A665-6.88*A649;
Chlorophyll b concentration (mg/L) Cb=24.96*A649-7.32*A665;
Chlorophyll=chlorophyll a+chlorophyll b.
The soil plantation Plantula Brassicae chinensis Chlorophyll content results of three kinds of different fertilizer process is as shown in table 2.
The soil plantation Plantula Brassicae chinensis Chlorophyll content results of table 2 three kinds of different fertilizer process
| The soil of different fertilizer process | Chlorophyll content (unit: mg/g) |
| The soil of Urea treatment | 1.82 |
| The soil of fertilizer process | 1.43 |
| The soil of calcium polypeptide process | 2.12 |
As can be seen from the result of table 2, the Plantula Brassicae chinensis Determination of Chlorophyll content that the calcium polypeptide promotor soil that applying the present invention obtains is planted is apparently higher than using the Plantula Brassicae chinensis Determination of Chlorophyll content that the soil of common urea, fertilizer is planted, therefore, the calcium polypeptide promotor that the present invention obtains can improve chlorophyll content of plant.
Application test example 3
Calcium polypeptide promotor the present invention obtained is applied to research plant soluble sugar being improved to effect.
Plantula Brassicae chinensis seed on the soil of application test example 1 is planted, ensure that every block seed number is on the ground roughly the same, after treating that it sprouts, win small and weak seedling, make its every block Chinese cabbage seedling on the ground the same, apply fertilizer when it grows to about 5cm, wherein a piece of land applies the calcium polypeptide accelerator solution of 100ml, 400ml(rainy day that simultaneously adds water can not add), the urea of the nitrogen quantity such as one piece of applying in other two pieces and water, the fertilizer of the nitrogen quantity such as another a piece of land applying, and apply appropriate phosphate fertilizer potash fertilizer to three blocks of soils, ensure that the phosphoric in these three blocks of soils is the same with potassium element.Within 40 days, measure edible portion solubility sugar degree later.Solubility saccharinity determining method: the 1. extraction of soluble sugar: get fresh edible portion, clean surface contaminants, shred mixing, take about 2g, put into scale test tube, add 5 ~ 10mL distilled water, plastic film sealing, in boiling water, extract 30min(extract 2 times), extracting liquid filtering enters in 25mL volumetric flask, repeatedly rinse test tube and residue, be settled to scale, measure absorption 0.5mL sample liquid (to repeat 3 times) in test tube, adding distil water 1.5mL, with the step of production standard curve, add phenol respectively in order, concentrated sulfuric acid solution, develop the color and measure optical density(OD).Obtained the amount of sugar by normal linearity equation, calculate soluble sugar content in test sample.Result is as shown in table 3.
Soluble sugar content result in the soil plantation Plantula Brassicae chinensis of table 3 three kinds of different fertilizer process
| The soil of different fertilizer process | Soluble sugar content (unit: %) |
| The soil of Urea treatment | 0.12 |
| The soil of fertilizer process | 0.13 |
| The soil of calcium polypeptide process | 0.15 |
As can be seen from the result of table 3, applying soluble sugar content in the Plantula Brassicae chinensis that the calcium polypeptide promotor soil that obtains of the present invention is planted higher than using soluble sugar content in Plantula Brassicae chinensis that the soil of common urea, fertilizer is planted, therefore, the calcium polypeptide promotor that the present invention obtains can improve plant soluble sugar content.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. one kind is improved the preparation method of the calcium polypeptide promotor of soil micro-ecosystem population quantity, it is characterized in that: described method will add in pyroreaction still containing protein raw materials, and add a certain amount of calcium oxide or unslaked lime and appropriate water in reactor after, be heated to 121 ~ 170 DEG C, insulation 0.5 ~ 6.5h, after being cooled to normal temperature, obtain described calcium polypeptide promotor, wherein, described is food factories mud containing protein raw materials, municipal sludge or plant cake, the calcium oxide of described interpolation or the weight of unslaked lime account for 10 ~ 35% of dehydration dewatered sludge or plant cake weight.
2. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 1, is characterized in that: described is plant cake containing protein raw materials, and Heating temperature is 121 ~ 130 DEG C.
3. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 1, is characterized in that: described is food factories mud or municipal sludge containing protein raw materials, and Heating temperature is 150 ~ 165 DEG C.
4. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to any one of claims 1 to 3, it is characterized in that: described heating adopts in reactor, passes into the direct-fired mode of water vapor, and the pressure of described water vapor is 0.6 ~ 0.9Mpa.
5. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to any one of claims 1 to 3, is characterized in that: the weight of the water of described interpolation is described containing 100% ~ 400% of protein raw materials weight.
6. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 1, is characterized in that: the calcium oxide of described interpolation or the weight of unslaked lime are 20% of described plant cake weight.
7. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 1, is characterized in that: the calcium oxide of described interpolation or the weight of unslaked lime are preferably 32% of dehydration dewatered sludge weight.
8. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 1, is characterized in that: described plant cake is any one or more in rapeseed cake, cottonseed cake, vinasse.
9. a kind of preparation method improving the calcium polypeptide promotor of soil micro-ecosystem population quantity according to claim 8, it is characterized in that: in described plant cake raw material, protein content is 38 ~ 45%, plant cake raw material pulverizing degree is 1 ~ 2mm.
10. for improving a fertilizer for soil micro-ecosystem population quantity, it is characterized in that: described fertilizer is the calcium polypeptide promotor utilizing method described in any one of claim 1 ~ 3 to prepare.
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| CN106831040A (en) * | 2016-12-01 | 2017-06-13 | 湖北大学 | It is a kind of to force biochemical cracking livestock and poultry cultivation night soil for calcium couples the method for compound and its application in arable farming |
| CN108975979A (en) * | 2018-08-28 | 2018-12-11 | 茆振斌 | The compound fertilizer as made from allantoin production waste liquid and preparation method thereof |
| CN108975978A (en) * | 2018-08-28 | 2018-12-11 | 茆振斌 | The processing method of allantoin production waste liquid |
| CN109020669A (en) * | 2018-08-28 | 2018-12-18 | 茆振斌 | A kind of processing method of allantoin production waste liquid |
| CN109134042A (en) * | 2018-08-28 | 2019-01-04 | 茆振斌 | It is a kind of as allantoin production waste liquid made from compound fertilizer and preparation method thereof |
| CN111187123A (en) * | 2020-02-03 | 2020-05-22 | 南阳师范学院 | Modifying agent, preparation method and method for promoting calcium absorption of crops in acid soil |
| CN111248227A (en) * | 2020-02-03 | 2020-06-09 | 湖北大学 | Modifying agent, preparation method thereof and method for promoting magnesium absorption of underground part of crop |
| CN111944726A (en) * | 2020-08-24 | 2020-11-17 | 广州市土根旺生物科技有限公司 | Soil microbial population regulator and preparation method and application thereof |
| CN115215704A (en) * | 2022-07-28 | 2022-10-21 | 无锡国联环保科技股份有限公司 | Novel application of sludge hydrolyzed polypeptide concentrated solution |
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| CN111248227A (en) * | 2020-02-03 | 2020-06-09 | 湖北大学 | Modifying agent, preparation method thereof and method for promoting magnesium absorption of underground part of crop |
| CN111944726A (en) * | 2020-08-24 | 2020-11-17 | 广州市土根旺生物科技有限公司 | Soil microbial population regulator and preparation method and application thereof |
| CN115215704A (en) * | 2022-07-28 | 2022-10-21 | 无锡国联环保科技股份有限公司 | Novel application of sludge hydrolyzed polypeptide concentrated solution |
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