KR102074384B1 - Cosmetic composition for improving wrinkles and skin elasticity comprising bifidobacterium animalis subsp. lactis lm1017 having immunostimulating activity - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin elasticity and wrinkle reduction, comprising Bifidobacterium animalis subsp. lactis LM1017 as an active ingredient. More specifically, the present invention provides a cosmetic composition for skin elasticity and wrinkle reduction, which contains novel Bifidobacterium animalis subsp. lactis LM1017 as an active ingredient, and stimulates skin fibroblasts to promote the production of type I procollagen.

Description

A cosmetic composition for improving wrinkles and skin elasticity, comprising a novel Bifidobacterium animal lactis LM1017 as an active ingredient TECHNICAL COMPOSITION FOR IMPROVING WRINKLES AND SKIN ELASTICITY COMPRISING BIFIDOBACTERIUM ANIMALIS SUBSP. LACTIS LM1017 HAVING IMMUNOSTIMULATING ACTIVITY}

The present invention relates to a cosmetic composition for wrinkle improvement and skin elasticity containing the novel Bifidobacterium animal lactis LM1017 as an active ingredient, and more specifically, to stimulate the production of type I procollagen by stimulating skin fibroblasts. It relates to a cosmetic composition for improving wrinkles and skin elasticity, containing a novel Bifidobacterium animal lactis LM1017 as an active ingredient to promote.

As the average life expectancy is extended due to the development of medicine, the desire to keep the youth as long as possible has become a common concern for both young and old, and the desire to prevent skin aging, which is most easily revealed, is increasing. Accordingly, research into the development of materials that can find the cause of skin aging and suppress it has been actively conducted. Skin aging is divided into two types depending on the cause. One is natural aging, and as the body ages, the body's immune system and physiological function decreases, so skin components such as hyaluronic acid and collagen cannot be recovered, resulting in decreased elasticity of the skin and dry wrinkles. do. Hyaluronic acid is one of the extracellular matrix components, which accounts for only 0.1 to 0.2% by dry weight, but forms a network with other components of the dermis to connect cells to each other and to deliver nutrients. At the same time, its strong binding to water molecules allows the skin to maintain a certain amount of moisture. Reduction of hyaluronic acid in the skin starts from 18 to 20 years of age and accelerates decomposition due to environmental pollution or UV exposure, and the reduction of hyaluronic acid in the dermis may cause skin elasticity, wrinkle formation and dry skin. Sometimes. For this reason, an approach to prevent aging by replenishing hyaluronic acid in the skin that has decreased with age has been taken. In addition, collagen accounts for 70 to 80% of the entire dermis, so degradation of collagen is closely related to decreased elasticity and wrinkle formation in the skin. Another type of skin aging is called aging that is caused by external stress, such as ultraviolet rays, called extrinsic aging. Ultraviolet rays synthesize vitamin D in the body and play a role in bactericidal action, but also cause dermatitis, skin cancer, sunburn, skin aging, dryness and fine lines. Ultraviolet (UV) is divided into three types, A, B, and C, depending on the wavelength, among which UVC is blocked in the ozone layer, and UVA and UVB affect the skin.

In particular, UVB (290 ~ 320nm), which is a medium wavelength, causes severe skin damage when exposed to acute or chronic exposure. Collagen, elastin, hyaluronic acid, etc. It destroys the skin and leads to loss of elasticity, resulting in coarse wrinkles. In addition, free radicals generated by UV rays damage the intracellular signaling system and increase the expression of collagen degrading enzyme MMP-1 (Matrix metalloproteinase-1) in the skin to destroy collagen.

The present application is to provide a novel anti-wrinkle and skin elastic cosmetic composition containing a novel Bifidobacterium animal lactis LM1017 as an active ingredient, which stimulates skin fibroblasts to promote production of type I procollagen.

However, the problem to be solved by the present application is not limited to the above-mentioned problem, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The first aspect of the present application, Bifidobacterium animal lactis LM1017 [ Bifidobacterium animalis subsp . Lactis LM1017, Deposited Organization: Korean Culture Center of Microorganisms (KCCM), Accession No .: KFCC11773P, Deposited Date: July 11, 2018], comprising a cosmetic composition for wrinkle improvement and skin elasticity to provide.

Collagen present in human skin is a major structural protein of the skin extra cellular matrix (ECM), which is responsible for skin elasticity and support of skin structure. Such collagen is synthesized into procollagen (procollagen) in the cell and then secreted out of the cell and polymerized into collagen fibers. However, the collagen is reduced by external factors such as age increase, ultraviolet irradiation and fine dust, which affects the wrinkle formation of the skin. Thus, according to embodiments herein, utilizing lactic acid bacteria microorganisms (Bifidobacterium animalis lactis LM1017, KFCC11773P), which have traditionally been proven safe in diet, stimulate skin fibroblasts to produce type I procollagen. By promoting the production has the effect of improving the elasticity and / or wrinkles of the skin.

1 is Bifidobacterium animal lactis LM1017 [ Bifidobacterium ] in one embodiment of the present application. animalis subsp . Lactis LM1017, Depositary Organization: Korean Culture Center of Microorganisms (KCCM), Accession No .: KFCC11773P, Deposited Date: July 11, 2018].
Figure 2 is a graph showing the effect of promoting the production of Type I procollagen of the cosmetic composition for wrinkle improvement and skin elasticity comprising the Bifidobacterium animal lactis LM1017 according to an embodiment of the present application in the CCD-986sk cell line.
Figure 3 is a graph showing the improvement of the wrinkles around the eye area wrinkles over time of the wrinkle improvement and skin elasticity cosmetic composition comprising the Bifidobacterium animal lactis LM1017 according to an embodiment of the present application in the wrinkle improvement effect clinical trial .
Figure 4 is a graph showing the change in the wrinkles under eye wrinkle improvement rate over time of the wrinkle improvement and skin elasticity cosmetic composition comprising Bifidobacterium animal lactis LM1017 according to an embodiment of the present application in the clinical trial of wrinkle improvement effect .
FIG. 5 is a graph showing skin elasticity improvement rate change over time of wrinkle improvement and skin elasticity cosmetic composition including Bifidobacterium animal lactis LM1017 according to an embodiment of the present application in a clinical trial for improving skin elasticity. to be.

DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present disclosure. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted for simplicity of explanation, and like reference numerals designate like parts throughout the specification.

Throughout this specification, when a part is said to be "connected" to another part, it includes not only "directly connected" but also "electrically connected" with another element in between. do.

Throughout this specification, when a member is located “on” another member, this includes not only when one member is in contact with another member but also when another member exists between the two members.

Throughout this specification, when a part is said to "include" a certain component, it means that it can further include other components, without excluding the other components unless otherwise stated. As used throughout this specification, the terms “about”, “substantially”, and the like, are used at, or in close proximity to, numerical values when manufacturing and material tolerances inherent in the meanings indicated are provided, and an understanding of the present application may occur. Accurate or absolute figures are used to assist in the prevention of unfair use by unscrupulous infringers. As used throughout this specification, the term “step of” or “step of” does not mean “step for”.

Throughout this specification, the term “combination (s) thereof” included in the expression of a makushi form refers to one or more mixtures or combinations selected from the group consisting of the components described in the expression of makushi form, It means to include one or more selected from the group consisting of the above components.

Throughout this specification, the description of “A and / or B” means “A or B, or A and B”.

Hereinafter, with reference to the accompanying drawings will be described in detail the embodiments and embodiments of the present application. However, the present disclosure may not be limited to these embodiments, examples, and drawings.

The first aspect of the present application, Bifidobacterium animal lactis LM1017 [ Bifidobacterium animalis subsp . Lactis LM1017, Deposited Organization: Korean Culture Center of Microorganisms (KCCM), Accession No .: KFCC11773P, Deposited Date: July 11, 2018], comprising a cosmetic composition for wrinkle improvement and skin elasticity to provide.

In one embodiment of the present application, the cosmetic composition for improving wrinkles and skin elasticity comprises the Bifidobacterium animal lactis LM1017 strain, its microorganisms, shreds, culture products, concentrates, or extracts as an active ingredient It may be, but may not be limited thereto. For example, the cosmetic composition for wrinkle improvement and skin elasticity may include heat treated Bifidobacterium animal lactis LM1017 strain as an active ingredient, but may not be limited thereto.

In one embodiment of the present application, lactic acid bacteria microorganisms themselves can be utilized by heat-treating the Bifidobacterium animal lactis LM1017 strain.

In one embodiment of the present application, by heat-treating the Bifidobacterium animal lactis LM1017 strain, it can lower the instability of the product by fermentation when the strain is applied to cosmetic products, but may not be limited thereto.

In one embodiment of the present application, the cosmetic composition for improving wrinkles and skin elasticity is not particularly limited if the content includes the Bifidobacterium animal lactis LM1017, for example, the Bifidobacterium animal About 1 × 10 ¹¹ cells / 100 g to about 5 × 10 ¹¹ cells / 100 g of Lys lactis LM1017 It may include a concentration of, but may not be limited thereto. For example, the concentration of Bifidobacterium animalis lactis LM1017 is about 1 × 10 ¹¹ cells / 100 g to about 5 × 10 ¹¹ cells / 100 g, about 1 × 10 ¹¹ cells / 100 g to about 4 × 10 ¹¹ cells / 100 g, about 1 × 10 ¹¹ cells / 100 g to about 3 × 10 ¹¹ cells / 100 g, about 1 × 10 ¹¹ cells / 100 g to about 2 × 10 ¹¹ cells / 100 g, about 2 × 10 ¹¹ cells / 100 g to about 5 × 10 ¹¹ cells / 100 g, about 2 × 10 ¹¹ cells / 100 g to about 4 × 10 ¹¹ cells / 100 g, about 2 × 10 ¹¹ cells / 100 g to about 3 × 10 ¹¹ cells / 100 g, about 3 × 10 ¹¹ cells / 100 g to about 5 × 10 ¹¹ cells / 100 g, about 3 × 10 ¹¹ cells / 100 g to about 4 × 10 ¹¹ cells / 100 g, or about 4 X 10 ¹¹ cells / 100 g to about 5 x 10 ¹¹ cells / 100 g, but may not be limited thereto.

In one embodiment of the present application, the Bifidobacterium animal lactis LM1017 may be to promote the production of collagen, but may not be limited thereto. For example, the Bifidobacterium animal lactis LM1017 may be to improve the elasticity of wrinkles generated in the skin damaged by ultraviolet exposure and / or skin damaged by the ultraviolet exposure by promoting the production of collagen, This may not be limited.

In one embodiment of the present application, the Bifidobacterium animal lactis LM1017 may be to stimulate the skin fibroblasts to promote the production of type I procollagen, but may not be limited thereto. For example, the Bifidobacterium animal lactis LM1017 stimulates skin fibroblasts to promote secretion of procollagen peptides and produces procollagen by cutting the procollagen peptides to improve skin wrinkles, or It may be to enhance the elasticity of the skin, but may not be limited thereto.

In one embodiment of the present application, the formulation of the cosmetic composition for wrinkle improvement and skin elasticity is not particularly limited, for example, in skins, lotions, essences, creams, packs, foundations and makeup bases It may have any one formulation selected, but may not be limited thereto.

In one embodiment of the present application, the wrinkle improvement and skin elasticity cosmetic composition may be added to the material selected arbitrarily selected according to the formulation or use of cosmetics, but may not be limited thereto. For example, purified water, oil, surfactants, moisturizers, higher alcohols, thickeners, chelating agents, pigments, fatty acids, antioxidants, preservatives, waxes, pH adjusters, flavoring agents, etc. may be added, but may not be limited thereto. have.

In one embodiment of the present application, when the formulation of the cosmetic composition for improving wrinkles and skin elasticity is a paste, cream or gel, as a carrier component animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol , Silicon, bentonite, silica, talc or zinc oxide, and the like, but may not be limited thereto.

In one embodiment of the present application, when the formulation of the cosmetic composition for improving wrinkles and skin elasticity is a powder or a spray, may include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component. In particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether, but may not be limited thereto.

In one embodiment of the present application, when the formulation of the cosmetic composition for improving wrinkles and skin elasticity is a solution or emulsion, the carrier component may include a solvent, a solubilizing agent or an emulsifying agent, for example, water, ethanol , But is not limited to isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan have.

In one embodiment of the present invention, when the formulation of the cosmetic composition for improving wrinkles and skin elasticity is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol Suspending agents such as esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant, and the like, but may not be limited thereto.

In one embodiment of the present application, when the formulation of the cosmetic composition for improving wrinkles and skin elasticity is a surfactant-containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, already Dazolinium derivatives, methyltaurate, sarcosinates, fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters, and the like. It may be, but may not be limited thereto.

Hereinafter, the present invention will be described in more detail with reference to examples of the present application, but the following examples are merely illustrated to aid the understanding of the present application, and the content of the present application is not limited to the following examples.

EXAMPLE

1. Preparation and Lactobacillus Lactobacillus

1-1. Bifidobacterium Animalless Lactis  Preparation of cosmetic composition for wrinkle improvement and skin elasticity containing LM1017

Bifidobacterium animal lactis LM1017 microorganisms according to this example were prepared by a batch culture method.

The lactic acid bacteria culture was carried out with a total amount of 60 L culture medium in a 100 L fermenter (Cobiotech), the stirring speed was 30 rpm, the temperature was maintained at 35 ℃ to 37 ℃, pH 5 to 6.5. After incubation, the culture medium was centrifuged at 13,000 rpm for 30 minutes to recover the cells. The recovered cells were diluted twice by weight with physiological saline (0.9% NaCl), lyophilized by 110 ° C for 10 minutes in autoclave, and then lyophilized, and finally heat-treated lactic acid bacteria (Bifidobacterium animal rock) Teeth LM1017) was obtained.

1-2. Collagen production promoting effect (in vivo)

The effect of promoting type I procollagen production on the heat treated Bifidobacterium animal lactis LM1017 obtained in the above examples was confirmed. The cell line was a CCD-986sk cell line, and inoculated at a concentration of 2 × 10 ⁴ cells / well in a 24-well cell culture plate. After incubation for 24 hours, the concentration of Bifidobacterium animal lactis LM1017 heat-treated in the cell line was 0 (negative control), 8 × 10 ³ , 8 × 10 ⁴ , 8 × 10 ⁵ , 8 × 10 ⁶ , 8 Each treatment was adjusted to x10 ⁷ cells / mL and incubated for 48 hours. Type I procollagen production was measured using the Procallagen Type I C-peptide EIA kit (TAKARA MK1010) to measure the amount of protein expressed in the cell line at 450 nm using a multi-plate reader. Compared in percentage.

As shown in FIG. 2, the heat treated Bifidobacterium animal lactis LM1017 showed an effective type I procollagen production promoting effect at a concentration of 8 × 10 ³ to 8 × 10 ⁸ cells / mL compared to the negative control ( p <0.05).

2. Manufacturing and application of human body containing cosmetics

2-1. Cosmetic manufacturing

The Bifidobacterium animal lactis LM1017 lactic acid bacteria microorganisms prepared in the above example were diluted within 2 to 10 times by weight to adjust the cell concentration to 1 × 10 ¹¹ cells / g.

In the compounding amounts shown in Table 1 below, control and experimental group serum were prepared by a conventional method.

2-2. Facial wrinkle improvement (in vivo )

Obtained in the above examples Wrinkle improvement and facial wrinkle improvement effect of the heat treatment Bifidobacterium animal lactis LM1017 as an active ingredient and the cosmetic composition for skin elasticity was confirmed through clinical trials. The study was conducted on 21 subjects who met the selection criteria and were not included in the exemption criteria and were applied three times a week by self-application method. The facial wrinkle improvement effect of the composition was confirmed by evaluation using Antera 3D and skin irritation evaluation by a dermatologist.

Figure 3 is a result showing the improvement of the wrinkles around the eyes of the cosmetic composition for wrinkles and skin elasticity by time. Compared to the placebo group as a control group, the wrinkle improvement and skin elasticity cosmetic composition of the present application was confirmed that the eye wrinkles significantly improved after use.

Figure 4 is a graph showing the wrinkle improvement rate under the eye of the cosmetic composition for wrinkle improvement and skin elasticity over time. Compared with the placebo group, which is a control group, the cosmetic composition for wrinkle improvement and skin elasticity of the present application showed that the visual evaluation score improved over time.

The above results, when applied to the skin Wrinkle improvement and skin elasticity cosmetic composition comprising the heat-treated Bifidobacterium animal lactis LM1017 of the present application as an active ingredient, it is effective in improving the tested area, that is, around the eyes and wrinkles under the eyes Prove that there is.

2-3. Skin elasticity improving effect (in vivo )

Obtained in the above examples Wrinkle improvement and skin elasticity improvement of the skin elasticity cosmetic composition comprising the heat treated Bifidobacterium animal lactis LM1017 as an active ingredient was confirmed through clinical trials. The experiment was conducted on 21 subjects who met the selection criteria and were not included in the exemption criteria. Skin elasticity improvement effect was confirmed by skin elasticity evaluation by Cutometer® and skin irritation evaluation by dermatologist.

5 is a result showing the skin elasticity improvement rate of the wrinkle improvement and skin elasticity cosmetic composition for each hour. Compared to the placebo group, which is a control group, the cosmetic composition for wrinkle improvement and skin elasticity of the present application showed that skin elasticity was remarkably improved after use. The above results were statistically significant (p <0.05) when the applied anti-wrinkle and skin elasticity cosmetic composition including the heat-treated Bifidobacterium animal lactis LM1017 as an active ingredient to the skin for 2 weeks. Prove that it improves elasticity.

The above description of the present application is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the following claims rather than the above detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present application.

Korea Microbial Conservation Center (Domestic) KFCC11773P 20180711

Claims (5)

Bifidobacterium animal lactis LM1017 ( Bifidobacterium animalis subsp. Lactis LM1017, Accession No .: KFCC11773P), comprising as an active ingredient, cosmetic composition for wrinkle improvement and skin elasticity.
The method of claim 1,
The cosmetic composition for improving wrinkles and skin elasticity is 1 × 10 ¹¹ cells / 100 g to 5 × 10 ¹¹ cells / 100 g of Bifidobacterium animal lactis LM1017. Containing in a concentration of, improve wrinkles and skin elasticity cosmetic composition.
The method of claim 1,
The Bifidobacterium animal lactis LM1017 is to promote collagen production, wrinkle improvement and skin elasticity cosmetic composition.
The method of claim 3, wherein
The Bifidobacterium animal lactis LM1017 is to stimulate the skin fibroblasts to promote the production of type I procollagen, wrinkle improvement and skin elasticity cosmetic composition.
The method of claim 1,
The wrinkle improvement and skin elasticity cosmetic composition is characterized in that it has any one of the formulations selected from skins, lotions, essences, creams, packs, foundations and makeup bases, wrinkle improvement and skin elasticity Cosmetic composition.

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