KR20140148166A - Cosmetic composition comprising the extract of Aronia melanocarpa and producing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of Aronia melanocarpa and having an antioxidative, whitening, or anti-wrinkle effect, and to a method for preparing the same. The present invention relates to an extract of Aronia melanocarpa prepared by subjecting Aronia melanocarpa to a low-temperature extreme cold process, followed by fermentation with lactic acid bacteria; to a functional cosmetic composition containing the extract as an active ingredient; and to a method for preparing the same. The extract of Aronia melanocarpa according to the present invention has gone through the low-temperature extreme cold process, such as a super high pressure or ultrasonic waves, thereby minimizing the destruction of useful active materials of Aronia melanocarpa itself. Furthermore, the extract of Aronia melanocarpa can be appropriately used as a cosmetic material by metabolizing the useful materials, which are eluted by the treatment with lactic acid bacteria, to induce the generation of various active materials. Furthermore, the cosmetic composition prepared by the method of the present invention has excellent antioxidant, whitening, moisturizing, and anti-wrinkle effects compared with when the general extraction method is performed and Aronia melanocarpa is subjected to an extreme cold process alone.

Description

[0001] The present invention relates to a functional cosmetic composition comprising an extract of Aronia as an active ingredient and a cosmetic composition comprising the extract of Aronia melanocarpa and a producing method thereof,

The present invention relates to a cosmetic composition having an antioxidative, whitening, moisturizing or wrinkle-reducing effect comprising an extract of Aronia as an active ingredient, and a process for producing the same.

With increasing living standards, interest in functional cosmetics to delay skin aging and maintain clean skin has increased and demand is rapidly increasing. The safety of cosmetics containing artificial chemicals has been emphasized, and functional cosmetics containing various natural products are in the spotlight due to the psychology of consumers who prefer natural and natural properties.

Pigmentation on the skin is one of the protective actions to protect the skin from ultraviolet rays. However, the skin pigmentation on the skin has a great influence on the beauty of women, and there are various administrations related to the whitening constantly. Especially, the use of cosmetics has led to a lot of management. In order to pursue beauty, there is an increasing interest in skin. Therefore, it is required to develop high-functional cosmetics that emphasize safety rather than merely protecting the skin. In addition, preference for whitening cosmetics in functional cosmetics is heightened, Research and markets for whitening cosmetics that lighten skin are on the rise.

In addition to pigmentation of the skin, active oxygen or free radicals break collagen, elastin and hyaluronic acid, which are connective tissues of the dermis, causing settlement phenomenon (wrinkles) in certain parts of the skin and oxidizing the lipid part of the cell membrane Causing skin diseases, acne, skin cancer and other diseases. In addition, the active oxygen is involved in spontaneous oxidation reaction during the melanin formation process, which causes the cause of spots and freckles, and causes wrinkles. Recently, studies on antioxidants that can regulate free radicals or free radicals have been actively carried out, and have been actively studied to investigate the effects of antioxidants such as superoxide dismutase (SOD), catalase, glutathione peroxidase and natural antioxidants Many antioxidants have been developed, including tocopherol, ascorbic acid, carotenoids, glutathione and synthetic antioxidants BHA and BHT. However, these drugs are not only expensive, but also have poor safety and stability in combination, making it difficult to obtain substantial effects.

Therefore, in order to improve the skin trouble caused by free radicals and inflammation, it is necessary to develop a cosmetic product which is excellent in antioxidative effect, has no side effects, is inexpensive, has excellent stability and is easy to use.

Korea Patent Publication No. 2011-0057954

Accordingly, an object of the present invention is to provide a method for producing a cosmetic composition comprising an extract of Aronia as an active ingredient, characterized in that it is prepared by a process of fermenting lactic acid bacteria after pre-treatment with ultrahigh pressure, ultrasonic pretreatment or ultra high pressure homogenization.

In addition, the present invention provides a cosmetic composition comprising an astaxanthin extract having antioxidative, whitening, moisturizing and wrinkle-reducing effects prepared by the above method as an active ingredient.

In order to accomplish the above object, the present invention provides a method for producing a catalyst, comprising the steps of: (a) (b) a high-pressure homogenization step of dispersing the dried aronia into nanoparticle size; (c) an extreme pretreatment step in which water or an organic solvent is added to the high pressure homogenized aronia and subjected to ultrahigh pressure and / or ultrasonic treatment; And (d) inoculating the pretreatment mixture with lactic acid bacteria and fermenting the mixture.

In one embodiment of the present invention, the fermenting step comprises inoculating the lactic acid bacterium with a concentration (v / v) of 1 to 30% based on the total weight of the pretreatment mixture, fermenting the mixture at 20 to 40 ° C for 6 to 72 hours After fermentation, the fermented Aronia was subjected to hot extraction for 2 to 24 hours at 40 to 100 ° C, followed by cold extraction at 4 to 15 ° C for 5 minutes to 24 hours, followed by filtration and concentration under reduced pressure. The concentrate was lyophilized, And a step of pulverizing.

In one embodiment of the present invention, the step (b) for extreme pretreatment may be performed by ultrasonication at 30 kHz to 120 kHz for 5 minutes to 1 hour and / or ultrasonic treatment at 4 ° C to 30 ° C for 5 minutes to 30 minutes at a pressure of 100 MPa to 500 MPa It can be treated at ultra high pressure.

In one embodiment of the present invention, the high-pressure homogenization in the step (c) may be performed by applying a pressure of 15,000 to 20,000 psi in the ultra-high pressure homogenizer through the microtubule in the apparatus three to five times.

In one embodiment of the present invention, the lactic acid bacteria of step (d) is Lactobacillus para casei (Lactobaillus paracasei) HS-05, Lactobacillus Lactobacillus rhamnosus ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ), Bifidobacterium longum ), yeast ( Saccharomyces exiguus ), Lactobacillus < RTI ID = 0.0 > acidophilus ), Annie Marlies Bifidobacterium (Bifidobacterium animalis ), Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ), Bulgarian bacillus ( Lactobacillus bulgaricus ), Lactobacillus casei ), Lactobacillus delbrueckii ), Lactobacillus ( Lactobacillus) plantarum), diacetyl Lactococcus lactis (Lactococcus diacetylactis , Lactococcus lactis), Streptococcus's Summer filler (Streptococcus thermophilus , Saccharomyces cerevisiae , Rhodopseudomonas spp. palustris , Aspergillus niger , and Acetobacter xylinum .

The present invention also provides a cosmetic composition comprising the extract of Aronia produced by the above method as an active ingredient.

In one embodiment of the present invention, the cosmetic composition may have antioxidant, anti-aging, whitening, wrinkle-improving or moisturizing effects.

The present invention relates to an extract of Aronia, a functional cosmetic composition comprising the extract as an active ingredient, and a process for producing the cosmetic composition, wherein the extract is prepared by fermentation with lactic acid bacteria after treating a low temperature extreme processing step with Aronia, The extract of Ahnia has minimized the destruction of useful active material of Ahnia itself because it has undergone extreme high pressure or ultra low temperature processing such as ultrasonic wave and the extract is dispersed into nano size by ultra high pressure homogenization process, It is effective to absorb. In addition, there is an effect of appropriately applying the extract of Aronia to the material of cosmetic materials by inducing the production of various active substances by metabolizing the useful substance eluted due to lactic acid fermentation treatment. In addition, the cosmetic composition prepared by the method of the present invention is more effective than antioxidants, whitening agents, moisturizing agents, and wrinkles, as compared with general extracting methods and extreme processing steps of Aronia.

FIG. 1 shows the result of measuring the cytotoxicity after treating the extract of Aronia with various methods (Experimental Example 1: General Aronia extract, Experimental Example 2: Extreme pretreatment Aronia extract, Experimental Example 3: Extreme pretreatment Aronia fermentation extract ).
FIG. 2 shows the result of measuring the antioxidant activity by DPPH scavenging after treating the extract of Aronia with various methods (Experimental Example 1: General Aronia extract, Experimental Example 2: Extreme pretreatment Aroma extract, Experimental Example 3: Extreme pretreatment Aronia fermented extract).
FIG. 3 shows the result of comparing the amount of melanin produced after treating the extract of Aronia with various methods (Experimental Example 1: General Aronia extract, Experimental Example 2: Extreme pretreatment Aronia extract, Experimental Example 3: Extreme pretreatment Aronia fermented extract ).

The present invention relates to a functional cosmetic composition comprising an actinic acid produced through a process of fermenting lactic acid bacteria after an ultrahigh pressure or ultrasonic wave pretreatment and an ultrahigh pressure homogenization treatment, wherein the cosmetic composition of the present invention has a DPPH free radical scavenging ability of 50% , Showing high melanin activity and exhibiting high moisture content and showing effects on antioxidation, whitening, wrinkle improvement and moisturizing. Thus, the present inventors have found that an extract of Ahnonia prepared by extreme pretreatment and lactic acid fermentation process as an active ingredient And is characterized by providing a functional cosmetic composition.

In recent years, not only many women but also men have become increasingly interested in cosmetics, and various functional cosmetics for skin whitening, acne, atopic dermatitis and wrinkle are being released. However, most functional cosmetics sold on the market are products using synthetic compounds, and cosmetic products containing synthetic compounds can cause sensitive skin reactions depending on the skin, which causes various skin side effects.

Therefore, the present inventors paid attention to Aronia while studying a substance having a functional effect useful for natural skin-derived skin.

Aronia is a fruit of Aronia tree which is used for edible or medicinal purposes. It is also used as a raw material for food coloring or as an ornamental plant. Red choke berry, black choke berry, and purple chokeberry are all known as Aronia. Aronia has anthocyanin content of 100mg per 320g, which is the highest of all plants in the natural world. Anthocyanin is noted for its antioxidant effect It is a substance to be received. The effect of anthocyanin has antioxidative effect, vision improvement effect and vascular disease prevention and improvement effect, but there is a disadvantage in that a loss of a useful substance that Aronia has is large when general hot water extraction is performed in Aronia.

Accordingly, the present invention provides a process for the preparation of (a) (b) a high-pressure homogenization step of dispersing the dried aronia into nanoparticle size; (c) an extreme pretreatment step in which water or an organic solvent is added to the high pressure homogenized aronia and subjected to ultrahigh pressure and / or ultrasonic treatment; And (d) inoculating the pretreatment mixture with lactic acid bacteria and fermenting the mixture.

The extreme pretreatment step in step (c) refers to ultrasonic treatment or ultrahigh-pressure treatment, and the same effect can be obtained by using one of the two methods.

The organic solvent may be a solvent selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane.

The lactic acid bacteria of step (d) is Lactobacillus para casei (Lactobaillus paracasei ) HS-05, Lactobacillus Lactobacillus rhamnosus ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ), Bifidobacterium longum ), yeast ( Saccharomyces exiguus ), Lactobacillus < RTI ID = 0.0 > acidophilus ), Annie Marlies Bifidobacterium (Bifidobacterium animalis ), Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ), Bulgarian bacillus ( Lactobacillus bulgaricus ), Lactobacillus casei ), Lactobacillus delbrueckii ), Lactobacillus ( Lactobacillus) plantarum), diacetyl Lactococcus lactis (Lactococcus diacetylactis), Lactococcus lactis (Lactococcus lactis), Streptococcus's Summer filler (Streptococcus thermophilus), with Saccharomyces Celebi Asia (Saccharomyces cerevisiae , Rhodopseudomonas spp. palustris , Aspergillus niger , Acetobacter xylinum , and the like, but are not limited thereto.

The inventors of the present invention measured the cytotoxicity of the extracts of the present invention by dividing them into general astaxanthin extracts, extreme pretreatment arnia extracts and extreme pretreatment arnia fermentation extracts, As a result, the cytotoxicity of less than 10% was observed in all three groups, and the lowest cytotoxicity was shown in the extreme pretreatment Aronia fermentation extract (see FIG. 1).

According to one embodiment of the present invention, the DPPH radical scavenging activity was measured in the above three groups. As a result, the scavenging ability was found to be 35% or more in all three groups. In particular, extreme pretreatment Aronia fermentation extract , The elimination ability of free radicals was found to exceed 50% in Experimental Example 3 (see FIG. 2).

According to another embodiment of the present invention, the amount of melanin produced was divided into general Aronia extract, extreme pretreatment Aronia extract and extreme pretreatment Aronia fermentation extract group. As a result, it was found that the highest amount of melanin produced in extreme pretreatment Aronia fermentation extract And the extreme pretreatment Aronia extract of Experimental Example 2 showed higher whitening activity than that of Example 1 which was the extract of Aronia (see FIG. 3).

According to another embodiment of the present invention, as a result of measuring the moisture content of 1,3-butylene glycol generally used in the above-mentioned three groups and comparative examples as a moisturizing cosmetic composition, The water content was higher than that of the comparative example in which 1,3-butylene glycol was known, and it was found that the highest moisture content was shown in Experimental Example 3, which is an extreme pretreatment fermentation extract of Aronia (see Table 2).

Based on these results, the inventors of the present invention have found that the extract of Aronia prepared by the method of the present invention is excellent in antioxidation, whitening, wrinkle improvement and moisturizing effect, and thus can be used for the production of functional cosmetics.

Therefore, the present invention can provide a cosmetic composition comprising an extract of Aronia as an active ingredient.

The cosmetic composition comprising the extract of Aronia according to the present invention as an active ingredient may be added in an amount of 0.0001 to 50% by weight based on the total weight of the cosmetic composition.

When the extract of Aronia is added in an amount of less than 0.0001% by weight based on the total weight of the cosmetic composition, it does not exhibit various physiological activity effects of the cosmetic composition of the present invention. On the other hand, when it is added in an amount exceeding 50% by weight, Or the desired effect can not be obtained in proportion to the added amount. Therefore, it is preferable to add the cosmetic composition in an amount within the range described above in consideration of economical efficiency, and the cost of the cosmetic composition according to the amount of the cosmetic composition increases.

When the composition of the present invention is prepared with a cosmetic composition, the composition of the present invention may contain not only the above-mentioned Aromania extract but also components commonly used in cosmetic compositions such as antioxidants, stabilizers, , Customary adjuvants such as pigments and flavoring agents, and carriers.

 The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The present invention also provides a cosmetic method characterized by applying the cosmetic composition of the present invention to human skin.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent atopy treatment effect according to the present invention can be used according to a conventional method of use, and the use frequency of the cosmetic composition can be changed according to a user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Manufacturing example  1>

Aronia  Ultra high pressure or ultrasonic pretreatment

The present inventors first rinsed the aronia with water for a short period of time in order to pre-treat the aronia with ultrahigh pressure or ultrasonic waves, and then dried in a hot air drier at 40 ° C for a day. To 200 g of the dried aronia, 1 L of water or an organic solvent was added Ultrasonic treatment was performed at a temperature of 20 ° C to 50 ° C for 5 minutes to 1 hour at 30 kHz to 120 kHz.

The inventors of the present invention carried out ultra-high pressure treatment at 100 to 500 MPa at 4 ° C to 30 ° C for 5 minutes to 30 minutes by placing 100 g of Aronia and 300 ml of water or organic solvent as described above in a plastic bag.

In the present invention, it is possible to manufacture by choosing one of a high-pressure pretreatment of Aronia and an ultrasound pretreatment of Aronia.

< Manufacturing example  2>

Aronia  Ultra high pressure homogeneous pretreatment

High pressure pre-treatment or ultrasonic pretreated argon was put into an ultra-high pressure homogenizer by the method of Preparation Example 1, and the pressure was applied at 15,000 to 20,000 psi and circulated through microtubules in the high pressure homogenizer three to five times Ahnia in solution was homogenized at high pressure with nanoparticle size.

< Manufacturing example  3>

Aronia's  Fermentation

A pre-treatment process of the Production Example 2 on a rough Chokeberry extract 1 to 20% Lactobacillus ramno suspension (Lactobacillus of rhamnosus ) were inoculated and fermented for 6 to 72 hours.

< Manufacturing example  4>

Aronia's  extraction

After 1 L of water or organic solvent was added to the fermented Aronia by the method of Preparation Example 3, the mixture was first subjected to heat extraction at a temperature of 40 ° C to 100 ° C for 2 hours to 24 hours, followed by heat extraction at 4 ° C to 15 ° C And extracted with ice for 5 minutes to 24 hours.

< Manufacturing example  5>

Aronia's  Extract Powdering

The extracted extracts were filtered and concentrated at room temperature using a rotary vacuum evaporator. The concentrated extracts were lyophilized and powdered.

< Example  1>

Aronia's  Cytotoxicity measurement results of extract

The present inventors have found that the To determine whether the extract of Aronia was suitable as a cosmetic composition, cytotoxicity was measured by dividing it into a general Aronia extract (control group), an extreme pretreatment Aronia extract and an extreme pretreatment Aronia fermentation extract group as shown in Table 1 below.

In Experimental Example 1, distilled water was added to dried Aronia to extract hot water. In Experimental Example 2, the dried Aronia was treated with ultrasound or ultrahigh pressure, passed through an ultrahigh pressure homogenizer, dispersed in nano size, , Experimental Example 3 was prepared by inoculating fermented lactic acid bacteria into crude aronia until ultra-high pressure homogenization by the same method as Experimental Example 2, followed by adding hot water to distilled water.

Cytotoxicity was measured by modifying the Mosmann method using 3- (4,5-dimethythiazo-2-yl) -2,5-dipheny-tetrazoliumbromide (MTT). CCD-986sk cells were inoculated in 96-well plates at a concentration of 1.5 × 10 4 cells / well, and then samples were added to each well at a culture time of about 80% and cultured in a CO 2 incubator for 24 hours. The MTT solution at 5 μg / ml was added to each well. After 4 hours, the supernatant was removed. 10 μl of acid-isopropanol (0.04 N HCl in isopropanol) was added to the microplate reader Tecan, USA). The absorbance was measured at a wavelength of 565 nm.

As a result, the cytotoxicity of not less than 10% was observed in all three groups of Table 1, and compared with Experimental Example 1, which is a general Aronia extract, Experimental Example 2, which is an extreme pretreatment arnoia extract, and extreme pretreatment Aronia fermentation It was found that the extract of Experimental Example 3 exhibited lower cytotoxicity (see FIG. 1).

< Example  2>

Aronia's  Extract Antioxidant ability  Measure

The inventors of the present invention measured the antioxidant activity of DPPH radical scavenging ability in order to determine whether the extract of Aronia produced by the methods of Production Examples 1 to 5 is suitable as a functional cosmetic material.

The scavenging activity of DPPH (α, α-diphenyl-β-picrylhydrazyl) radicals was tested by modifying Dietz's method. 200 μl of a 0.1 mM DPPH solution prepared by using ethanol as a solvent in a 96-well plate and 80 μl of a sample of the experimental example were mixed and allowed to stand in a dark room at 25 ° C for 20 minutes, and the absorbance was measured at 525 nm. On the other hand, ascorbic acid was used as a positive control in this experiment.

As a result, the elimination ability of 35% or more was measured in all three groups of Table 1, and in particular, in Experimental Example 3, which is an extreme pretreatment Aronia fermentation extract, the scavenging ability of free radicals was close to 60%, and skin aging It can be used as a cosmetic composition having an effect of preventing (see Fig. 2).

< Example  3>

Aronia's  Measurement of skin whitening efficacy of extract

The present inventors have confirmed melanin production amounts in order to determine skin whitening efficacy of the extract of Aronia produced by the methods of Production Examples 1 to 5 above.

The amount of melanin production was measured using a mouse-derived melanocyte (Clone-M3 cell line) producing melanin The whitening activity of each sample was measured and the experimental method was as follows. First, Clone-M3 cell line was inoculated at a concentration of 1.5x10 cells / well in 96 wells and treated for 3 days at a concentration of 0.5 mg / mL for 24 hours and then for 3 days. After 3 days, the medium of each well was removed, washed with PBS, and 1 ml of 1N NaOH was added to each well, followed by stirring to dissolve the melanin component. The absorbance at 400 nm was measured using a microplate reader in order to measure the content of melanin. The results showed that the whitening activity was the highest in Experiment 3, which was an extreme pretreatment fermentation extract of Aronia, and that the extreme pretreatment Example 2 also showed high whitening activity compared to Example 1, which was an extract of Aronia obtained by the conventional extraction process (see FIG. 3).

< Example  4>

Aronia's  Measurement of skin moisturizing effect of extract

In order to examine whether the extract of Aronia produced by the methods of Preparation Examples 1 to 5 exhibits skin moisturizing effect, the present inventors diluted the Aronia in Experimental Examples 1 to 3 with distilled water to a concentration of 0.5 mg / mL in Petri dishes Add 20 mL of diluent The sample was allowed to stand in a desiccator containing CaCl 2 at a relative humidity of 41% and at a temperature of 25 ° C, and then the amount of anilia diluted solution was measured over time to measure the change with time. As a result of measuring the moisturizing activity of each sample using 1,3-butylene glycol generally used in a moisturizing cosmetic composition as a comparative example, it was found that in all three groups of Table 1, 1,3-butylene And the highest moisture content was shown in Experimental Example 3 which is an extreme pretreatment Aronia fermentation extract (see Table 2).

Therefore, according to the results of Examples 1 to 4, extreme pretreatment (ultrahigh pressure or ultrasonic) fermented extract of Aronia can be used as various cosmetic compositions due to its effect of not only antioxidant but also whitening and moisturizing wrinkles. And the effect was much higher than that of conventional extracts of Aronia.

< Example  5>

Aronia's  Extract Cosmetic  Produce

The present inventors confirmed that the extreme pre-treatment Aronia fermentation extract prepared by the methods of Production Examples 1 to 5 was suitable as a functional cosmetic with antioxidative, whitening and moisturizing effects, and was actually made into a cosmetic.

<5-1> Cream manufacturing

The inventors of the present invention prepared a cream with the composition shown in Table 3 below.

<5-2> Essence manufacturing

The present inventors prepared an essence with the composition shown in Table 4 below.

<5-3> Lotion manufacturing

The inventors of the present invention produced lotion water with the composition shown in Table 5 below.

<5-4> Lotion manufacturing

The present inventors manufactured lotions with the compositions shown in Table 6 below.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

(a) drying the aronia;
(b) a high-pressure homogenization step of dispersing the dried aronia into nanoparticle size;
(c) an extreme pretreatment step in which water or an organic solvent is added to the high pressure homogenized aronia and subjected to ultrahigh pressure and / or ultrasonic treatment; And
(d) inoculating the pretreatment mixture with lactic acid bacteria and fermenting the mixture, wherein the method comprises the step of: The method according to claim 1,
Wherein the fermentation step comprises inoculating the lactic acid bacteria so as to have a concentration (v / v) of 1 to 30% based on the total weight of the pretreatment mixture, fermenting the mixture at 20 to 40 ° C for 6 to 72 hours, Extracting by hot water extraction at 100 ° C for 2 to 24 hours, followed by cold extraction at 4 to 15 ° C for 5 minutes to 24 hours, followed by filtration and concentration under reduced pressure, followed by lyophilization of the concentrate to pulverize the crude extract Wherein the cosmetic composition comprises an extract of Aronia as an active ingredient. The method according to claim 1,
The extreme pretreatment step (b) is ultrasonic treatment at a frequency of 30 kHz to 120 kHz for 5 minutes to 1 hour and / or ultrahigh pressure treatment at a pressure of 100 MPa to 500 MPa at 4 ° C to 30 ° C for 5 minutes to 30 minutes. A method for producing a cosmetic composition comprising an extract as an active ingredient. The method according to claim 1,
Wherein the high-pressure homogenization of step (c) is performed by applying a pressure of 15,000 to 20,000 psi in an ultra-high pressure homogenizer through the microtubules in the device for 3 to 5 times. Gt; The method according to claim 1,
The lactic acid bacteria of step (d) is Lactobacillus para casei (Lactobaillus paracasei ) HS-05, Lactobacillus Lactobacillus rhamnosus ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ), Bifidobacterium longum ), yeast ( Saccharomyces exiguus ), Lactobacillus &lt; RTI ID = 0.0 &gt; acidophilus ), Annie Marlies Bifidobacterium (Bifidobacterium animalis ), Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ), Bulgarian bacillus ( Lactobacillus bulgaricus ), Lactobacillus casei ), Lactobacillus delbrueckii ), Lactobacillus ( Lactobacillus) plantarum), diacetyl Lactococcus lactis (Lactococcus diacetylactis), Lactococcus lactis (Lactococcus lactis), Streptococcus's Summer filler (Streptococcus thermophilus), with Saccharomyces Celebi Asia (Saccharomyces cerevisiae , Rhodopseudomonas spp. palustris , Aspergillus niger , and Acetobacter xylinum . The method for producing a cosmetic composition according to claim 1, A cosmetic composition comprising an extract of Aronia produced by the method according to any one of claims 1 to 5 as an active ingredient. The method according to claim 6,
The cosmetic composition according to claim 1, wherein the cosmetic composition has an antioxidant, anti-aging, whitening, wrinkle-improving or moisturizing effect.

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