KR102068079B1 - Cosmetic composition for promoting recovery of skin injury without skin irritation - Google Patents

Abstract

The present invention relates to an effective cosmetic composition for promoting the recovery of damaged skin without skin irritation. More specifically, the present invention relates to an effective cosmetic composition for promoting the recovery of damaged skin without skin irritation, comprising a stem and leaf extract of Hibiscus trionum, and additionally a Drosera rotundifolia extract, a Flammulina velutipes extract and a stem and leaf extract of Adenophora triphylla as active ingredients.

Description

Cosmetic composition for promoting recovery of damaged skin without skin irritation {Cosmetic composition for promoting recovery of skin injury without skin irritation}

The present invention relates to an effect cosmetic composition that promotes the recovery of damaged skin without skin irritation, and more specifically, it is effective to extract the stem and leaf extract of watermelon grass and additionally the spatula extract, the hackberry extract and the stem leaf extract of the remnant. It relates to an effect cosmetic composition that promotes recovery of damaged skin without skin irritation included as a component.

Inflammatory response in the skin begins as a protective action against skin damage caused by physical stimuli, chemicals, bacteria, etc. In response, keratinocyte or Langerhans cell (Rangeshans cell) Inflammation response is initiated by the export of various types of cytokine, Interleukin-8 (IL-8), Interleukin-6 (IL-6), Interleukin-1 (Interleukin-1; IL-1), Tumor necrosis factor-α (TNF-α), and the activated macrophages (nitricoxide (NO) or prostaglandin E2) Excessive production of (PGE2) deepens the inflammatory process.

Fine dust is generally referred to as dust having a particle size of 10 μm or less, abbreviated as PM10 (Particulate Matter 10), and classified into PM2.5 (Particulate Matter 2.5) which is ultra fine dust of 2.5 μm or less. It mainly occurs when burning fossil fuels, and fine dust from China with 70% dependence on coal accounts for a large part. In addition, yellow sand is a phenomenon in which small sand, loess or dust of deserts and ocher areas in the centers of Asian continents such as China and Mongolia floats in the sky and flies far away by upper winds and travels to Jeju Island with long-range mobile air pollutants. This is possible.

In industrialized countries, approximately 25-33% of diseases for the environment and caused by factors in Europe has been estimated that annually about 310,000 people die prematurely in the polluted air, the American Cancer Society ultra-fine dust is 10 per m ³ Increasing the µg results in a 7% increase in overall mortality. In 2013, a joint study by the Ministry of Environment (Journal of Allergy and Clinical Immunology. 132 (2), 2013, 495-498) showed that dermatitis symptoms worsened when the concentration of fine dust or air pollutants increased. .

The average fine dust (PM10) is 1㎍ / m ³ increased and the symptoms of dermatitis average increase of 0.4% each time, as the air pollutants, total volatile organic compounds (TVOC) and benzene which is one of increased by 0.1ppb each symptom It increased 2.59% and 2.74%. In addition, when yellow dust occurs, the dust pollutes the atmosphere with yellowish brown, and the amount of dust in the air increases by an average of four times, and the skin is easily tired and loses its vitality. In addition, dust dust is smaller than ordinary dust and can penetrate deep into the pores of the skin, causing skin problems, and worsening various symptoms such as dry skin, irritant dermatitis, allergic dermatitis, acne, blemishes, and freckles. Therefore, the occurrence of dermatitis caused by yellow dust and fine dust is expected to increase, the need for a material to mitigate the skin inflammatory response caused by yellow dust and fine dust will be increased.

In order to treat skin inflammation, steroids are mainly used, but the use of non-steroids has recently increased due to various side effects such as skin atrophy and the possibility of growth retardation during prolonged administration. However, non-steroidal agents also exhibit side effects such as erythema and itching and weakened immunity, making it difficult to treat dermatitis. Accordingly, many studies have been made to find new substances having high therapeutic effect and low side effects from natural materials derived from safety. Representatively, rowan extract (registered patent 10-0563548), gall bladder extract (registered patent 10-1309172), spirulina extract (published patent 10-2009-0079468), liberation wind extract (registered patent 10-0919852), etc. No extract has been developed that has the effect of relieving dust-induced inflammation.

Therefore, there is an increasing need for new substances having high therapeutic effect and low side effects from natural materials derived from safety.

1. Republic of Korea Patent No. 10-0563548 2. Republic of Korea Patent No. 10-1309172 3. Republic of Korea Patent Publication No. 10-2009-0079468 4. Republic of Korea Patent No. 10-0919852

Thus, the present inventors conducted an intensive study on the natural-derived material that promotes the recovery of damaged skin without skin irritation, and confirmed the effects of the stem and leaf extracts of watermelon grass. In the case of further including the extract and the stem leaf extract of the remnant as an active ingredient to prove the synergistic effect was completed the present invention.

Therefore, the problem to be solved of the present invention is to include the stem and leaf extract of watermelon grass and additionally the spatula extract, hackberry extract and stem leaf extract of the remnant as an active ingredient to promote the recovery of damaged skin without skin irritation It is to provide an effect cosmetic composition.

The present invention to achieve the above technical problem,

It provides an effective cosmetic composition for promoting the recovery of damaged skin without skin irritation, characterized in that the stem and leaf ethanol extract of watermelon pool as an active ingredient.

Cosmetic composition according to the present invention as described above may further comprise a sticky spatula ethanol extract.

The cosmetic composition according to the present invention as described above may further include a mushroom ethanol extract.

Cosmetic composition according to the present invention as described above may further comprise a stem and leaf ethanol extract of the stem.

In the cosmetic composition according to the present invention as described above, the stem and leaf ethanol extract of the watermelon, the stick spatula ethanol extract, the mushroom ethanol extract and the stem and leaf ethanol extract of the stem is 1: 1: 1: 1 It is preferably included in the ratio.

delete

The cosmetic composition according to the present invention is effective in promoting the recovery of damaged skin without skin irritation, so that the cosmetic composition can be usefully used as a cosmetic.

Hereinafter, specific contents for carrying out the present invention will be described.

The present invention includes a stem and leaf extract of watermelon grass as an active ingredient provides an effect cosmetic composition for promoting the recovery of damaged skin without skin irritation.

The watermelon is the annual name of Hibiscus trionum.

In another embodiment, the present invention may further comprise a sticky spatula extract as an active ingredient, the sticky spatula is a perennial herb of the genus Drosera rotundifolia as a string earrings.

As another embodiment, the present invention may further include the extract of Sesame mushroom as an active ingredient, the sesame mushroom is a mushroom of the fungus creased fungus of the genus Pleurotus eryngii, scientific name is a mushroom cultivated as edible Flammulina velutipes.

In another embodiment, the present invention may further comprise a stem and leaf extract of the stem, which is named Adenophora triphylla var. japonica HARA. is a perennial herbaceous plant belonging to the family Campanula.

In the present invention, the extraction solvent of the stem and leaves of the watermelon pool, sticky spatula, hackberry, stem and leaf extract of the watermelon as described above may use water, lower alcohol of C1 to C4 or a mixed solvent thereof, the lower The alcohol can be ethanol or methanol, more specifically ethanol.

In addition, as the extraction method, conventional methods in the art such as filtration, hot water extraction, dipping extraction, reflux cooling extraction, ultrasonic extraction, supercritical extraction, etc. may be used without particular limitation.

When the composition according to the present invention includes both the stem and leaf extract of the watermelon, the sticky spatula extract, the hackberry extract and the stem and leaf extract of the twig, it is preferably included in a weight ratio of 1: 1: 1: 1. .

Cosmetic compositions in the present invention may include components conventionally used in cosmetic compositions, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

Such cosmetic compositions may be prepared in any formulations commonly prepared in the art. In one embodiment, the formulation may be any one selected from the group consisting of a high viscosity emulsifier type, a low viscosity emulsifier type, and a solubilized formulation, but is not limited thereto. Depending on the formulation to be prepared may comprise a cosmetic composition blending components commonly used in the art. Examples include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays, etc. It may be, but is not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, sun lotion, sun cream, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick shape product, balm ( Balm) type product, may be prepared in the form of a spray or powder.

Depending on the formulation to be prepared, water phase components, oil phase components, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, preservatives, fragrances and the like can be selected and added.

The extract constituting the active ingredient of the cosmetic composition according to the present invention may be included as 0.001 to 30% by weight relative to the weight of the composition when used alone or when used in combination of two to three components. When the extract is less than 0.001% by weight relative to the total composition weight, there is no anti-pollution effect through alleviation of skin troubles such as skin inflammation, and when the extract is more than 30% by weight, the degree of increase in the effect of increasing the content is minimal. There is a problem with formulation stability and it is not economical.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples. However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example Preparation of Extracts According to the Invention

The stem and leaves of the crushed watermelon grass 10Kg was mixed with 100L of 70% ethanol.

The mixed raw material was extracted by immersion at room temperature for 7 days, and then filtered through 250 mesh and 0.5 μm filter paper to obtain a liquid extract. The obtained liquid extract was used to obtain watermelon extract using a rotary vacuum concentrator and a lyophilizer.

Sticky spatula extract, Sesame mushroom extract, and stem and leaf extract of twigs were prepared in the same manner. Samples for the experiments described later were as follows.

Example 1: Watermelon Pool Extract

Example 2: watermelon grass extract + sticky spatula extract (weight ratio 1: 1)

Example 3: Watermelon Pool Extract + Hackberry Mushroom Extract (weight ratio 1: 1)

Example 4: Watermelon Pool Extract + Straw Extract (Weight Ratio 1: 1)

Example 5 watermelon grass extract + sticky spatula extract + hackberry extract + twig extract (weight ratio 1: 1: 1: 1)

Experimental Example  1: skin safety test

In order to verify the skin safety for the sample of the example, MTT assay experiments on fibroblasts (HDF), melanocytes (B16F10), keratinocytes (HaCaT) were performed. The sample of Example was suspended in purified water by concentration (50 mg / L), respectively, and cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were each dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and incubated for 48 hours at 37 ° C., 5% CO ₂ . It was.

After removing the culture medium and incubating the sample for 48 hours in a concentration-treated medium, the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and incubated for 24 hours at 37 ° C. and 5% CO ₂ . 100 L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and the absorbance was measured at 540 nm. Table 1 shows the results.

Example 100 (μg / ml) One 2 3 4 5 Fibroblasts (HDF) 99 ± 0.8 99 ± 0.6 99 ± 0.6 99 ± 0.7 99 ± 0.8 Melanocytes (B16F10) 99 ± 0.5 99 ± 0.5 99 ± 0.8 99 ± 0.6 99 ± 0.4 Keratinocytes (HaCaT) 99 ± 0.8 99 ± 0.6 99 ± 0.9 99 ± 0.8 99 ± 0.8

Referring to Table 1, each sample did not exhibit cytotoxicity. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

Experimental Example 2: Skin Damage Recovery Effect

The 50 women were divided into 5 groups of 10 people each, causing skin irritation through tape stripping. Subsequently, the group without any treatment was used as a control, and Examples 1 to 4 were applied twice daily in the morning and afternoon, and the degree of recovery of skin damage was observed. The results are shown in Table 2 below.

Skin Damage Recovery Time Control 7.5 days Example 1 4.8 days Example 2 4.1 days Example 3 4.1 days Example 4 3.9 days Example 5 3.3 days

Referring to Table 2, it was confirmed that the recovery time of the skin damage is significantly shortened by the sample of the example.

Experimental Example 3: Skin Irritation Test

Separately, 30 women in their 20s were subjected to primary skin irritation tests in accordance with CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association Inc Washington, DC, 20036, 1991). 20 μl of the test substance (10% in patch base) prepared in the Finn Chamber was added dropwise, washed with 70% ethanol and dried, and then placed on the back of the test site to form a micropore tape. Fixed. After 24 hours, the pin chamber was removed and the skin change was read visually. The results are shown in Table 3.

The average responsiveness in Table 3 was calculated according to the following equation, skin irritation determination after 24 hours patch--no erythema or peculiar phenomenon, ± is slightly redder than the surroundings, + is significantly redder than the surroundings, ++ is more severe than the surroundings Decided to be red and swollen. Determination of skin irritation is grade I (non-irritating range) if the average response is 0 to 0.1, grade II (light stimulation range) if 0.11 to 0.3, grade III (medium stimulation range) if 0.31 to 0.5, grade IV or higher. (Strong stimulus range).

Equation: average responsiveness = [(±) number * 1 + (+) number * 2 + (++) number * 3] / number of subjects

Judgment result Average reactivity Judgment grade - ± + ++ Example 1 29 One - - 0.03 I Example 2 29 One - - 0.03 I Example 3 29 One - - 0.03 I Example 4 29 One - - 0.03 I 5 to implementation 28 2 - - 0.06 I

Referring to Table 3, almost no skin irritation was observed in the portions to which Examples 1 to 5 were applied, and it was confirmed that the irritation determination grade I was safe for the skin as there was no occurrence of skin irritation.

As described above, it is apparent that the composition according to the present invention exhibits an effect of promoting recovery of damaged skin without skin irritation.

Claims (7)

Injured skin without skin irritation, characterized by the weight ratio of 1: 1 and 1: 1 ethanol extract of stem and leaf ethanol extract, sticky spatula ethanol extract, hackberry mushroom ethanol extract and stem and leaf ethanol extract of watermelon Effect cosmetic composition to promote recovery.



delete delete delete delete delete delete