KR102007469B1 - Method for the production of niosome with idebenone or coenzymeQ10 and cosmetic composition therewith - Google Patents

Abstract

The present invention relates to a cosmetic composition including noisome containing idebenone or coenzyme Q10. The problem of poor solubility of idebenone or coenzyme Q10 was solved by encapsulating idebenone or coenzyme Q10 using a mixed surfactant mixed with polyglyceryl-10-dioleate, PEG-40 hydrogenated castor oil, hydrogenated lecithin, and glycine soja (soybean) sterol. Therefore, in the present invention, it is possible to produce a cosmetic composition of a transparent aqueous formulation containing idebenone or coenzyme Q10, and produce a cosmetic composition of an emulsion formulation having formulation stability. In addition, the present invention provides a cosmetic composition having excellent skin transdermal absorption, excellent skin elasticity improvement, and excellent skin transparency improvement by ensuring the long-term stability of idebenone in cosmetics.

Description

Method for preparing niosome containing idebenone or coenzyme Q10 and cosmetic composition comprising same {Method for the production of niosome with idebenone or coenzymeQ10 and cosmetic composition therewith}

The present invention relates to a method for preparing niosomes containing idebenone or coenzyme Q10 and to a cosmetic composition comprising the same, and more particularly, to using a mixed surfactant to increase and stabilize the solubility of idebenone or coenzyme Q10. Alternatively, the co-enzyme Q10-encapsulated niosomal bi-layer vesicles are prepared, and a cosmetic composition including the same is provided and provided. That is, polyglyceryl-10-dioleate, PG-40 hydrogenated castor oil, and hydrogenatidrecithin (Hydrogenated lecithin), a technique for forming a niosomal bi-layer vesicle encapsulating idebenone and coenzyme Q10 using a complex surfactant mixed with dolkongsterol.

Idebenone is generally known to be useful for the treatment of Alzheimer's disease as a medicine. When cerebrovascular disorders occur, a lot of glutamic acid is produced at the nerve endings, and calcium in the nerve cells is introduced into the cells, resulting in a lack of intracellular reducing material, glutathione, which oxidizes and destroys the cells. It is reported to exhibit the effect of suppressing the destruction of the mitochondria by removing the free group.

Idebenone is also widely used as a raw material for cosmetics.It is also called 'hydroxydecylubiquinone', and it is excellent in promoting cell growth, cell protection, and antioxidant effects, and promotes regeneration of not only collagen and elastin but also moisture layers in the dermis. It plays a role. In addition, it has a function of inhibiting melanin production, reducing skin pigmentation or brightening the skin to inhibit pigmentation of the skin, and is effective in removing elasticity, wrinkles and improving skin tone.

However, it has been reported that idebenone is insoluble in water, difficult to dissolve in oils used in cosmetics, and partially soluble in some organic solvents such as DMSO, acetone, and ethanol. However, these solvents have a limitation that they cannot be contained in cosmetics. Therefore, idebenone is mainly used in health foods and medicines, and it is difficult to use a lot in the cosmetic industry. In cosmetics, it is limited to emulsifiers such as creams and emulsions, but it suffers from difficulties due to solubility due to precipitation. Especially, it is not actively used because no manufacturing method is developed to ensure stability while dissolving transparently. I can't.

Republic of Korea Patent No. 10-0785484 (Registration Date: 2007.12.06) relates to a base composition nano-encapsulated high concentration of idebenone, a preparation method thereof and a cosmetic containing the same, a solvent having at least two hydroxyl groups in the idebenone It is to provide a base composition and a method for preparing the encapsulated phospholipids, ceramides, cholesterol and oil-soluble antioxidants, emulsified oil, water-soluble antioxidants in a high concentration of idebenone mixed in an appropriate ratio according to the order. Republic of Korea Patent No. 10-1521868 (Registration Date: 2015.05.14) relates to a method for producing a wrinkle improvement cosmetic composition comprising an idebenone capsule encapsulated in a skin lipid complex and moisturizing oil and a method for stabilizing idebenone, The present invention provides an anti-wrinkle cosmetic composition comprising a skin lipid complex and an idebenone capsule enclosed in a moisturizing oil, and a method of manufacturing the same.

The present invention is a polyglyceryl-10-dioleate, PIG-40 hydrogenated castor oil (PEG-40 hydrogenated castor oil), hydrogenated lecithin, dolkongsterol It is intended to develop and provide a niosomal bi-layer vesicle containing Idebenone and a cosmetic composition containing the same by using a mixed mixed surfactant. That is, a method for preparing a complex surfactant that can make a niobium containing Idebenone and coenzyme Q10 into a transparent formulation even when dispersed in water, and a cosmetic and oil-containing composition in the liquid-containing bi-layer niosomes It is an object of the present invention to develop an emulsion in which the endoplasmic reticulum is formed.

The present invention is 10 to 40% by weight of polyglyceryl-10 dioleate, 10 to 40% by weight of PEG-40 hydrogenated castor oil, 5 to 30% by weight of hydrogenated lecithin, 2 to 20% by weight Surfactant lipid bases comprising dolkongsterol and 0.1-30% by weight of idebenone or coenzyme Q10 are provided.

In addition, the present invention comprises the steps of dissolving polyglyceryl-10 dioleate and PEG-40 hydrogenated castor oil at 80 ~ 90 ℃ (a); After step (a), dissolving at 80-90 ° C. by adding hydrogenetirecithin; After step (b), adding dolkongsterol to dissolve at 95-130 ° C .; After step (c), dissolving at 90-120 ° C. and swelling at 90-120 ° C. for 1-4 hours by adding idebenone or coenzyme Q10; And after step (d), degassing while cooling (e).

The present invention also provides a cosmetic composition to which the surfactant lipid base of the present invention is added.

In addition, the present invention comprises the steps of dissolving the surfactant lipid base of claim 1 (a '); After step (a '), adding and dissolving an oil phase (b'); After step (b '), adding and dissolving an aqueous phase (c'); After the step (c '), adding and mixing the purified water as a solvent (d'); And after step (d '), adding and mixing a gelling agent (e'); It provides a method for producing a cosmetic composition to which the surfactant lipid base is added, comprising a.

At this time, the production method of the present invention, preferably after the addition of purified water addition, may further comprise the step of adding an additive used in cosmetics.

In addition, the production method of the present invention, may further comprise the step of passing through a microfluidizer (M / F) before the gelling agent is added.

In the present invention, polyglyceryl-10-dioleate (Polyglyceryl-10-dioleate), PG-40 hydrogenated castor oil (PEG-40 hydrogenated castor oil), hydrogenated lecithin (Hydrogenated lecithin), dolkongsterol ( Idebenone or coenzyme Q10 was encapsulated using a mixed surfactant mixed with glycine soja (soybean sterol) to solve the problem of poor solubility of idebenone or coenzyme Q10.

Therefore, in the present invention, the cosmetic composition of the transparent aqueous formulation containing idebenone or coenzyme Q10 can be prepared, and in the composition in which the oil phase is mixed, an emulsified liquid crystal structure can be formed. In addition, the present invention by providing a long-term stability of idebenone in cosmetics, it provides a cosmetic composition having an excellent skin transdermal absorption, excellent skin elasticity improvement and excellent skin transparency (whitening cosmetics) improving efficacy.

1 is a diagram showing the molecular structure and molecular weight of idebenone and coenzyme Q10.
2 is a manufacturing process diagram of an idebenone-containing complex surfactant lipid base.
FIG. 3 is a process diagram for preparing a cosmetic formulation using an idebenone containing surfactant lipid base.
Figure 4 is a schematic diagram showing the bi-layer (bi-layer) of the niosome prepared in various forms.
5 is a schematic diagram showing the structure of a bi-layer vesicle (niosome).
6 is a schematic diagram in which a bi-layer is formed into a flat autostructure.
7 is a schematic diagram in which a bi-layer is formed into a spherical autostructure, that is, a vesicle (niosomes).
8 shows niosomes that are transparently stabilized with the final product (cosmetic composition).
9 is a graph showing the results of evaluating long-term stability of the present invention.
10 is a graph showing the results of the dermal transdermal absorption of the present invention.
11 is a graph showing the results of measuring skin elasticity improving effect of the present invention.
12 is a graph showing the results of measuring skin transparency improvement effects of the present invention.

Idebenone is generally used as a medicine for the treatment of Alzheimer's disease, and is known to be mainly used in health foods and medicines. In addition, it is known to play a role in promoting cell growth, cytoprotective, antioxidant effects, etc. in the dermis to promote the regeneration of not only collagen and elastin but also moisture layer.

Idebenone may be useful in the cosmetics industry due to such characteristics, but is insoluble in water, difficult to dissolve in oils used in cosmetics, and is not easily used due to solubility due to precipitation. Accordingly, the present invention intends to develop and provide a method capable of ensuring stability while dissolving idebenone transparently.

In the present invention, the present invention is 10 to 40% by weight of polyglyceryl-10 dioleate, 10 to 40% by weight of PEG-40 hydrogenated castor oil, 5 to 30% by weight of hydrogenated lecithin, 2 ~ Surfactant lipid bases are provided comprising 20% by weight of dolkongsterol and 0.1-30% by weight of idebenone or coenzyme Q10.

In addition, the present invention comprises the steps of dissolving polyglyceryl-10 dioleate and PEG-40 hydrogenated castor oil at 80 ~ 90 ℃ (a); After step (a), dissolving at 80-90 ° C. by adding hydrogenetirecithin; After step (b), adding dolkongsterol to dissolve at 95-130 ° C .; After step (c), dissolving at 90-120 ° C. and swelling at 90-120 ° C. for 1-4 hours by adding idebenone or coenzyme Q10; And after step (d), degassing while cooling (e).

The present invention also provides a cosmetic composition to which the surfactant lipid base of the present invention is added.

In addition, the present invention comprises the steps of dissolving the surfactant lipid base of claim 1 (a '); After step (a '), adding and dissolving an oil phase (b'); After step (b '), adding and dissolving an aqueous phase (c'); After the step (c '), adding and mixing the purified water as a solvent (d'); And after step (d '), adding and mixing a gelling agent (e'); It provides a method for producing a cosmetic composition to which the surfactant lipid base is added, comprising a.

Polyglyceryl-10-dioleate used in the present invention is a diester of an unsaturated fatty acid oleic acid (olleic acid) and polyglycerol-10 (polygelycerin-10). Polyglyceryl-10-dioleate is used as a skin conditioning agent (softener) and a surfactant (emulsifier), and is widely used as a raw material for sebum (PEG), and is immiscible by adsorbing on the interface of the components of cosmetics It is also used as a raw material to help disperse and emulsify them.

PEG-40 hydrogenated castor oil is a component of a polyethyleneglycol derivative of hydrogenated castor oil containing an average of 40 moles of ethylene oxide. It was derived from the product obtained by hydrogenating (caster oil). Fiji-40 Hydrogenated Castor Oil is used as a fragrance or surfactant (emulsifier or emulsifier).

Hydrogenated lecithin is a phospholipid obtained by adding hydrogen to soybean phospholipid, and is obtained by adding hydrogen to lecithin derived from soybeans and eggs. Organize cells to help form healthy cell membranes. Hydrogenetidrecitin is used as a surfactant (emulsifier), skin conditioning agent, suspending agent (non-surfactant), and helps maintain moisture for long periods of time, helping to moisturize skin, and revitalizing and healthy skin with skin conditioning effect It is effective in improving.

Glycine soja (soybean) sterol is a mixture of phytosterols obtained from Soybean Glycine Soja, which is used in cosmetics as a skin conditioning agent (softener). In 2017, it changed its name from consterol to dol consterol.

On the other hand, niosome (Niosome) is to form a bimolecular film using a non-ionic surfactant, to explain the difference between the niosome (Niosome) and liposomes (Liposome) as follows. The liposomes in the biofilm are those that form bimolecular films using phospholipids. It is a constituent of phospholipids, ceramides, cholesterol, and fatty acids. On the other hand, niosomes form bimolecular membranes using non-ionic surfactants, which have been mentioned as problems of phospholipids (long-term preservation, prone to rancidity, difficult to obtain, expensive, and long-term stability of bimolecular membranes). It is a good alternative to liposomes in the cosmetic industry.

In the present invention, the mixed surfactant used as a phospholipid substitute is 10 to 40% by weight of polyglyceryl-10 dioleate, 10 to 40% by weight of PEG-40 hydrogenated castor oil, and 5 to 30% by weight of hydrogeney. It can be obtained by mixing thirecithin and 2-20% by weight of dolkongsterol, and by mixing and dissolving 0.1-30% by weight of idebenone or coenzyme Q10, the idebenone (or coenzyme Q10) surfactant lipid of the present invention The base can be manufactured. This can stably pack difficult to dissolve Idebenone or Coenzyme Q10 inside the surfactant.

In order to test the solubility of the lipid base thus prepared, 1-20% by weight of an idebenone surfactant lipid base was dissolved in 20% by weight of a polyol mixed with 50:50 of glycerin and dipropylene glycol, followed by dissolution. To prepare a cosmetic composition of 100% by weight of the total amount by filling with purified water, it can be seen that the yellow liquid is prepared as a transparent liquid. In order to make the result more transparent, the result was passed through a high pressure mixer called a high pressure microfluidizer, whereby a composition having a more transparent particle size and tube can be obtained. At this time, in the operating conditions of the high pressure microfluidizer (emulsifier), the pressure passing through the nano chamber is preferably 3,000 to 20,000 psi. . Under these conditions, the particle size distribution may form a fine bi-layer vesicle (niosome) of 10 to 120nm. Idebenone or coenzyme Q10 is enclosed in the niosome (vegicle) produced in this way.

On the other hand, the present invention can also be developed in the form of an emulsifier, such as cream, the milky white emulsifier composition may be used 1 to 20% by weight of the idebenone or coenzyme Q10 containing surfactant lipid base, various non-polar The ester oil, vegetable oil, silicone oil can be mixed and used in the above method. At this time, an emulsifier can also be added as needed. These emulsions may not necessarily use a high pressure mixer, it is possible to obtain an emulsion having a particle size distribution of 1 ~ 50㎛.

On the other hand, in the present invention, the cosmetic composition, for example, solutions, suspensions, emulsions, pastes, lotions, gels, water-soluble liquids, creams, essences, surfactant-containing cleansing, oils, oil-in-water (O / W) Basic cosmetic formulations selected from the group consisting of water-in-oil (W / O) type; skin; Lotion; Eye cream; Soothing Gel; Ointment; Formulation for mask pack; Body wash formulations; Peeling gel; Oil-in-water and water-in-oil makeup bases; foundation; Skin cover; Color cosmetic formulations selected from lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils; Scalp formulations; It may be any one selected from among, and preferably at least one formulation selected from the group consisting of transparent skin toner, transparent essence, low viscosity emulsion lotion essence, high viscosity emulsion type cream, but preferably transparent skin toner, transparent It is preferably used in one or more formulations selected from the group consisting of essences, low viscosity emulsion lotions, essences, high viscosity emulsion creams, sheet masks. More preferably, it can be used in lotions, essences, creams, eye creams or sheet masks for any one of the increase of transdermal absorption, moisturizing effect, antioxidant effect, skin elasticity improvement.

Moreover, the cosmetic composition of this invention can be used overlapping with other cosmetic compositions other than this invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.

[Examples 1-3: Idebenone or Coenzyme Q10-containing surfactant lipid base preparation]

Surfactant lipid base compositions are described via Examples 1-3 and compared with Comparative Example 1. Example 1 shows a surfactant lipid base composition capable of stabilizing idebenone and Example 2 coenzyme Q10. The examples presented below are described with a total content of 100% by weight, so that anyone with a major in cosmetics and chemistry can easily understand.

Idebenone has the chemical name Hydroxydecyl Ubiquinone (C ₁₉ H ₃₀ O ₅ , 339.44 grams / mol), Coenzyme Q10 is Ubidecarenone (C ₅₉ H ₉₀ O ₄ , 863.49 grams / mol) It has a chemical name of, and as a derivative relationship based on the ubiquinone structure (Idebenone is a material that combines hydrophobicity having a hydroxy decyl group), it has a molecular structure and molecular weight as shown in FIG. These two components are poorly soluble in both hydrophilic solvents and lipophilic solvents, making them difficult to use in the cosmetics industry and thus are not used in transparent formulations.

Idebenone or Coenzyme Q10 containing surfactant lipid base Name Example 1
(weight%) Example 2
(weight%) Example 3
(weight%) Comparative Example 1
(weight%) Polyglyceryl-10 Dioleate 25 25 25 - PEG-40 Hydrogenated Castor Oil 25 25 25 - Hydrogenated Lecithin 20 20 20 - Dol consterol 10 10 10 - Idebenon 20 - 10 20 Coenzyme Q10 - 20 10 - Phospholipids (phospholipids) - - - 25 Ceramide - - - 25 cholesterol - - - 10 Triglyceride - - - 20 sub Total 100 100 100 100

In this example, 25% by weight of polyglyceryl-10dioleate, 25% by weight of PEG-40 hydrogenated castor oil, 20% by weight of hydrogenetirescitin, based on 100% by weight total After mixing 20% by weight of dolkongsterol, 20% by weight of idebenone was mixed and dissolved to prepare an idebenone surfactant lipid body base. Further, Example 2 was prepared by mixing and dissolving 20% by weight of coenzyme Q10. In addition, Example 3 was prepared by mixing and dissolving 10% by weight of idebenone and 10% by weight of coenzyme Q10. On the other hand, in Comparative Example 1, an idebenone-containing lipid base was prepared close to liposomes using phospholipids (the experimental results show that when it is combined with phospholipids, idebenones coalesce to form a stable phase).

Figure 2 shows a method for producing a surfactant lipid base containing the idebenone. This method is a method for stabilizing idebenone in a surfactant, the step of dissolving polyglyceryl-10 dioleate and PEG-40 hydrogenated castor oil at 80 ~ 90 ℃; After step (a), dissolving at 80-90 ° C. by adding hydrogenetirecithin; After step (b), adding dolkongsterol to dissolve at 95-130 ° C .; After step (c), dissolving at 90-120 ° C. and swelling at 90-120 ° C. for 1-4 hours by adding idebenone or coenzyme Q10; And after step (d), degassing while cooling (e). In this case, coenzyme Q10 may be encapsulated in the same manner, and idebenone and coenzyme Q10 may be encapsulated at the same time.

[Examples 4-8: Preparation of Example 1 containing cosmetic composition]

On the other hand, using the idebenone surfactant lipid base of Example 1 prepared as described above to prepare a cosmetic prototype with various compositions shown in Table 2. Examples 4 to 5 are aqueous formulations without an oil phase, Example 6 is a formulation using a small amount of oil phase (3%), and Examples 7 to 8 are formulations using 9 to 30% oil phase.

Example 1 Cosmetic Composition  Name Example 4
(weight%) Example 5
(weight%) Example 6
(weight%) Example 7
(weight%) Example 8
(weight%) Comparative Example 2
(weight%) Lipid body Example 1
Idebenone surfactant lipid base 5 10 25 10 35 - Comparative Example 1 - - - - - 10 Paid Ester oil - - One 3 10 - Vegetable oil - - One 3 10 - Dimethicone - - One 3 10 - Awards Glycerin: Dipropylene Glycol 50:50 Mixture One 5 30 5 10 5 Solvent Purified water Quantity Quantity Quantity Quantity Quantity Quantity additive EDTA-2Na 0.05 0.05 0.05 0.05 0.05 0.05 Allantoin 0.1 0.1 0.1 0.1 0.1 0.1 Purslane extract 0.1 0.1 0.1 0.1 0.1 0.1 Gelling agent Clear Xanthan Gum 0.1 0.1 0.1 0.3 0.3 0.1 sub Total 100 100 100 100 100 100 Exterior Yellow red
Transparent liquid Yellow red
Transparent liquid Yellow red
Transparent liquid Yellow red
Suspension Yellow red
Suspension Yellow red
Suspension pH (pH meter) 5.85 6.14 6.25 5.75 5.82 5.97 Average particle size nm (particle measuring instrument) 45.2 38.6 32.5 198.5 375.5 980.7 Specific gravity (weight measurement) 1.17 1.15 1.19 1.12 1.13 1.19 Stability (45 ° C 4 weeks) Stable Stable Stable Stable Stable Detached

To describe in detail a method for preparing a cosmetic composition according to an embodiment of the present invention carried out with the composition as follows. Dissolving Example 1 (a '); After step (a '), adding and dissolving an oil phase (b'); After step (b '), dissolving by adding an aqueous phase (glycerine + dipropylene glycol) (c'); After the step (c '), adding and mixing the purified water as a solvent (d'); And after step (d '), adding and mixing a gelling agent (e'); It is configured to include. At this time, in the present embodiment, after the addition of purified water, the additive was added, and after mixing the additive (before adding the gelling agent) to make fine particles, a high pressure microfluidizer (M / F) was used. Passed. At this time, the particle size produced was 70 ~ 990nm. 3 schematically shows the process in a drawing.

Figure 4 shows the idebenone encapsulation stabilization form of the present invention prepared in a variety of formulations (oil-in-water, water-in-oil) depending on the use and the amount of oil phase. A is an oil-in-water emulsion (o / w), B is a water-in-oil emulsion (w / o), C is a water-in-oil emulsion (w) / o) is the formulation of the polymer dispersed in, D is a figure showing the formation of niosomes. Dispersion of the mixed surfactant lipid base in water can produce a flat bilayer, which is stirred with a homomixer to form a spherical bilayer vesicle (niosome).

As a result of the preparation of the cosmetic prototype in the above manner, Examples 4 to 6 were prepared in a transparent formulation, and Examples 7 to 8 were prepared in a formulation having an emulsion of emulsion type because they contained an oil phase. In addition, it was confirmed that Examples 4 to 8 of the present invention are superior in terms of stability than the composition of Comparative Example 2 prepared through Comparative Example 1 using phospholipids. More specifically, Examples 4-6 could be prepared in a transparent formulation, which appeared as a dark yellow red liquid under the influence of idebenone. Such a technique capable of producing a transparent formulation that is stable for a long time is very useful for developing cosmetics and thus has a very good invention value.

On the other hand, the combination of polyglyceryl-10 dioleate, PEG-40 hydrogenated castor oil, hydrogenetidrecithin and dol consterol, which are the compositions of Example 1, is an essential component for forming the niosomes of the present invention. A very stable bi-layer phase is formed by including both polyglyceryl-10 dioleate with two alkyl chains, hydrogenedeticithin, and PEG-40 hydrogenated castor oil with one alkaline chain and dol consterol. . In particular, Example 1 and the aqueous phase and the solvent, and after adding the additive and passing the high-pressure microfluidizer from 2 to 6 times at 5,000 ~ 20,000psi to form a transparent nanoemulsion, and also the oil phase 3 ~ Inclusion of 30% by weight can form a fine niosomal emulsion (Table 2 results above).

FIG. 5 is a diagram for easily explaining the structure of a niobium manufactured by the present invention. When a bilayer having a flat structure is stirred with a homomixer, a spherical bilayer vesicle (niosome) as shown in FIG. 5 is formed. . Idebenone may be packed in a vesicle in the form embedded in the hydrophilic portion or anchored to the hydrophobic portion.

6 shows in detail the mechanism structure by which the mixed surfactant lipid base of the present invention is formed in a bi-layer. A stable bi-layer can be obtained by mixing a component having two alkyl chains of polyglyceryl-10 dioleate and hydrogenedrescithin with PEG-40 hydrogenated castor oil and dol consterol. As shown in FIG. 6, spontaneous self-regulatory bodies may be formed to encapsulate and stabilize idebenone or coenzyme Q10.

Figure 7 shows in detail the schematic diagram of the bilayer autostructure formed in a niobium form by stirring. Hydrophilic and hydrophobic moieties with stable molecular arrangements as a result of mixing two alkyl chains of polyglyceryl-10 dioleate and hydrogenedetisitine with PEG-40 hydrogenated castor oil and dol consterol It can be arranged in between, the idebenone is stably packed in between can be maintained even in long-term storage. In addition, it can dissolve and disperse transparently even when mixed with water.

8 is a photograph of the final product obtained by encapsulating the idebenone of Example 5 in a bilayer of a spherical niobium. As shown in FIG. 8, it has a dark yellow red color due to the characteristics of the drug idebenone, and when dispersed in water, it was found to be dissolved in yellow and transparently dispersed. The photo on the left is a concentrate having 10% concentration of idebenone, and the photo on the right shows that it is dissolved in a 1% aqueous solution. It was found to be easily dissolved in a yellow transparent aqueous solution.

[Examples 9-11: Preparation of the cosmetic composition of the Example 1 containing transparent formulation]

It was confirmed that the present invention can be produced in a transparent formulation through the above examples, in this example, the cosmetic muffle formulation of the transparent formulation was prepared in Examples 9 to 11, and compared with Comparative Example 3.

When applying the base obtained in Example 1 as in Example 9, it had a transparent particle diameter of 43nm on average, it was possible to develop an ample of transparent type in appearance.

Example 1 Transparent Formulation Cosmetic Composition Name Example 9
(weight%) Example 10
(weight%) Example 11
(weight%) Comparative Example 3
(weight%) Lipid body Example 1
Idebenone surfactant lipid base 5 10 25 - Comparative Example 1 - - - 10 Awards Glycerin: Dipropylene Glycol 50:50 Mixture 5 7 10 5 Solvent Purified water Quantity Quantity Quantity Quantity Addition 1 EDTA-2Na 0.05 0.05 0.05 0.05 Allantoin 0.1 0.1 0.1 0.1 Purslane extract 0.1 0.1 0.1 0.1 Gelling agent Clear Xanthan Gum 0.1 0.1 0.1 0.1 Addition 2 Adenosine 0.04 0.04 0.04 0.04 Niacinamide 2 2 2 2 Ethyl ascorbic acid One One One One Sodium Hyaluronate 1% Solution One One One One Turmeric Root Extract 0.05 0.05 0.05 0.05 sub Total 100 100 100 100 Exterior Yellow red
Transparent liquid Yellow red
Transparent liquid Yellow red
Transparent liquid Yellow red
Suspension pH (pH meter) 5.5 5.2 5.7 5.9 Average particle size nm (particle measuring instrument) 43 37 32 995 Specific gravity (weight measurement) 1.07 1.05 1.09 1.05 Stability (45 ° C 4 weeks) Stable Stable Stable Detached

[Examples 12-14: Preparation of Example 1 containing emulsion cosmetic (Emulsion type cream composition)]

Through the above examples, it was confirmed that the present invention can be produced as a fine emulsion containing a lot of oil phase. In this example, Examples 12 to 14 were prepared and compared with Comparative Example 4. This experiment showed that Idebenone, which has excellent skin efficacy but was insoluble in powder, could be easily used in lotions or cream cosmetics made with emulsions.

Example 1 emulsion containing cosmetics Name Example 12
(weight%) Example 13
(weight%) Example 14
(weight%) Comparative Example 4
(weight%) Lipid body Example 1
Idebenone surfactant lipid base 5 10 25 - Comparative Example 1 - - - 10 Awards Glycerin: Dipropylene Glycol 50:50 Mixture 5 7 10 5 Solvent Purified water Quantity Quantity Quantity Quantity Paid Cetearyl Acetate 3 5 4 3 Purified Stearic Acid 2 One 2 2 Triethylhexanoin One 3 2 One Macadamia Seed Oil 3 One 2 3 Pumpkin Seed Oil 5 7 8 5 Cetylethylhexanoate 2 2 2 2 Tocopheryl acetate 0.2 0.2 0.2 0.2 Addition 1 EDTA-2Na 0.05 0.05 0.05 0.05 Allantoin 0.1 0.1 0.1 0.1 Purslane extract 0.1 0.1 0.1 0.1 Gelling agent Clear Xanthan Gum (1% solution) 0.1 0.1 0.1 0.1 Carbomer 940 (2% solution) 12 12 12 12 Addition 2 Adenosine 0.04 0.04 0.04 0.04 Niacinamide 2 2 2 2 Ethyl ascorbic acid One One One One Sodium Hyaluronate (1% solution) One One One One Turmeric Root Extract 0.05 0.05 0.05 0.05 sub Total 100 100 100 100 Exterior Yellow-red
Latex Yellow-red
Latex Yellow-red
Latex Yellowish red
Emulsion (separation) pH (pH meter) 5.5 5.2 5.7 5.9 Average particle size nm (particle measuring instrument) 793 662 538 5,370 Specific gravity (weight measurement) 0.998 0.992 0.995 1.05 Viscosity (cps, RV Viscometer, No. 6, 12 rpm, 1 minute) 38,500 58,800 48,200 39,900 Stability (45 ° C 4 weeks) Stable Stable Stable Detached

Experimental Example 1: Evaluation of long-term stability, dermal transdermal absorption, elasticity and transparency]

1) Long-term stability evaluation of Idebenon

 Example 9 and Comparative Example 3 and Example 12 and Comparative Example 4 were put into a 45 ℃ incubator for 1 month to evaluate the stability. It was shown in Figure 9 to evaluate the stability through HPLC quantitative analysis. As shown in FIG. 9, it was found that the content of idebenone in the transparent type essence composition of Example 9 and the emulsion type cream composition of Example 12 was more than 95% quantified and stable. In Comparative Examples 3 and 4, the composition was Idebenone content was also reduced to 31.5% and 29.7%, respectively, indicating that stability was not obtained.

2) Evaluation of Skin Transdermal Absorption of Idebenone

 Percutaneous absorption was evaluated with Example 9, Comparative Example 3, and Example 12 and Comparative Example 4.

The device used in the experiment was a Logan Franz Diffusion cell Semi-Auto system, model BFC-1512, and the membrane used was Merck MILLIPORE Strat-M. Membrane). This method is the most common method of measuring transdermal absorption, and after 8 hours, the sample is taken and quantitatively analyzed by HPLC, and the results are shown graphically in FIG. 10.

As a result of quantitative analysis, each sample was measured three times and the average value was indicated. As shown in Figure 10, after 8 weeks the transdermal absorption of Comparative Example 3 and Comparative Example 4 showed an absorption of about 8.9 ~ 11.5%. On the other hand, in Examples 9 and 12, after 8 weeks, it showed a high transdermal penetration effect (51.5 ~ 56.3%).

From these results, it can be seen that Examples 9 and 12 show a superior percutaneous absorption ability than Comparative Examples 3 and 4.

3) Evaluation of skin elasticity improvement

The skin elasticity improvement effect was compared with Example 12 and Comparative Example 4.

Evaluation method was used to measure the skin elasticity of Aramo TS (Korea). Each sample was applied to the entire face twice a day (morning and evening) for 4 weeks and the same spot was determined and the skin elasticity after 4 weeks was measured. Ten subjects were selected for each sample, measured three times, and the average value was applied to quantify the final result.

As a result of measuring the skin elasticity improvement effect, as shown in Figure 11, in Comparative Example 4 was 15.3% after 3 weeks, 17.5% after 4 weeks, Example 12 was 29.7% after 3 weeks, 32.5% after 4 weeks. As a result, it was confirmed that Example 12 shows an improvement in skin elasticity than Comparative Example 4.

4) Evaluation of Skin Transparency (Whitening) Improvement

In Example 12 and Comparative Example 4, the skin transparency improvement effect was compared.

The evaluation method was performed by measuring the clearness of the skin using a Derma View Pro skin meter. For the experiment, each sample was applied twice a day (morning and evening) for 4 weeks by the same amount, and the same spot was set and measured after 4 weeks of skin clearness. Ten subjects were selected, measured three times, and the average value was applied to quantify the final results.

As a result of the skin transparency test, as shown in Figure 12, Comparative Example 4 was 17.5% at the beginning, 19.2% after 1 week, 25.3% after 3 weeks, 27.6% after 4 weeks, Example 12 was 17.8% at the beginning, 26.4 after 1 week %, 47.5% after 3 weeks, and 53.9% after 4 weeks. As a result, it was confirmed that Example 12 was clearer than the Comparative Example 4, and the skin and freckles were improved.

Claims (6)

10-40 weight percent polyglyceryl-10 dioleate, 10-40 weight percent PEG-40 hydrogenated castor oil, 5-30 weight percent hydrogenetirescitin, 2-20 weight percent dolkongsterol and Surfactant lipid base comprising 0.1-30% by weight of idebenone or coenzyme Q10.
Dissolving polyglyceryl-10 dioleate and PEG-40 hydrogenated castor oil at 80-90 ° C. (a);
After step (a), dissolving at 80-90 ° C. by adding hydrogenetirecithin;
After step (b), adding dolkongsterol to dissolve at 95-130 ° C .;
After step (c), dissolving at 90-120 ° C. and swelling at 90-120 ° C. for 1-4 hours by adding idebenone or coenzyme Q10; And
After step (d), degassing while cooling (e); method of producing a surfactant lipid base comprising a.
Cosmetic composition to which the surfactant lipid base of Claim 1 was added.
Dissolving the surfactant lipid base of claim 1 (a ');
After step (a '), adding and dissolving an oil phase (b');
After step (b '), adding and dissolving an aqueous phase (c');
After the step (c '), adding and mixing the purified water as a solvent (d'); And
After step (d '), adding and mixing a gelling agent (e'); Method for producing a cosmetic composition to which the surfactant lipid base is added, comprising a.
The method of claim 4, wherein
The manufacturing method,
Method of producing a cosmetic composition to which the surfactant lipid base is added, further comprising the step of adding the additive used in cosmetics after the addition of purified water.
The method of claim 4, wherein
The manufacturing method,
A method of producing a cosmetic composition to which a surfactant lipid base is added, further comprising the step of passing a microfluidizer (M / F) before the gelling agent is added.

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