KR101875355B1 - 목적 단백질을 세포 외로 분비시키기 위한 시그널 펩타이드 암호화 서열을 포함하는 발현 벡터 라이브러리 - Google Patents
목적 단백질을 세포 외로 분비시키기 위한 시그널 펩타이드 암호화 서열을 포함하는 발현 벡터 라이브러리 Download PDFInfo
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Abstract
본 발명은 5'에서 3' 방향으로 프로모터, 제1 제한효소 인식 서열, 코작 서열, 시그널 펩타이드 암호화 서열, 제2 제한효소 인식 서열 및 poly A 서열을 순차적으로 포함하는, 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터에 관한 것으로, 시그널 펩타이드 암호화 서열 및 목적 단백질을 암호화하는 서열을 용이하게 삽입하고 교체할 수 있는 제한효소 인식 부위를 포함하고 있어, 단시간에 다양한 발현 벡터를 제조할 수 있다. 또한, 본 발명은 800개의 시그널 펩타이드 암호화 서열이 클로닝된 발현 벡터 라이브러리를 제공하며, 상기 발현 벡터 라이브러리를 이용하여 시그널 펩타이드 간의 목적 단백질의 세포외 분비량을 비교할 수 있으므로 목적 단백질에 적합한 시그널 펩타이드를 탐색하는 데에 유용하게 사용될 수 있다.
Description
본 발명은 목적 단백질을 세포 외로 분비시키기 위한 시그널 펩타이드 암호화 서열을 포함하는 발현 벡터 및 그 라이브러리에 관한 것이다.
재조합 단백질 의약품은 유전자 재조합 기술을 이용하여 생체에서 얻기 힘든 치료용 단백질 성분을 미생물이나 동물세포시스템을 통해 대량생산한 의약품을 말한다. 재조합 단백질은 세포 내에 발현시키거나 세포 외로 분비되도록 조절할 수 있다. 세포 내 발현 시 단백질의 과발현으로 인하여 세포 내에 비활성화 상태의 불용성 덩어리로 축적되는 경우가 많으며, 그렇지 않다 하더라도 세포를 파쇄하는 번거로움과 세포 내에 존재하는 수많은 단백질들과 분리하기 위한 정제공정의 어려움 등 생산성을 저하시키는 많은 요인들을 제공한다. 반면에 생성된 단백질을 세포 외로 분비시킬 경우 전자의 경우에 따르는 어려움을 쉽게 회피할 수 있다. 또한 단백질의 세포외 분비는 전사 후 단백질의 접힘 및 수식이 정확하게 이루어졌을 때만 가능하므로 치료용 단백질의 생산 시 활성화된 형태의 정확한 3차구조를 가진 가용성 단백질을 얻을 수 있는 장점이 있다. 따라서 재조합 단백질을 세포 외로 분비될 수 있도록 생산하는 것은 단백질의 생산수율 측면뿐만 아니라 단백질의 품질관리(quality control) 측면에서도 유리하다. 그러므로 재조합 단백질 의약품을 제조하는 데에 있어, 재조합 단백질을 효율적으로 세포 밖으로 분비시킬 수 있는 최적화 단계가 중요하다.
시그널 펩타이드는 분비 단백질이나 막단백질의 N말단에 위치하여 분비성 단백질 또는 막 단백질의 표적화(targeting) 신호로 기능하는 아미노산 서열로, 현재까지 알려진 시그널 펩타이드의 종류만 6천여 가지로 매우 다양하다. 목적 단백질을 생산함에 있어서, 어떠한 시그널 펩타이드를 적용하느냐에 따라 목적 단백질의 세포외 분비율이 달라질 수 있으며, 이와 관련하여 목적 단백질에 맞는 시그널 펩타이드를 선정하여 목적 단백질 세포외 분비를 최적화하는 연구가 보고된 바 있다(Acta Biochim Biophys Sin (Shanghai) (2011) 43 (2): 96-102, Signal peptide replacements enhance expression and secretion of hepatitis C virus envelope glycoproteins. Biochem Biophys Res Commun. 2010 Jan 1;391(1):931-5, Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system.).
이에, 본 발명자들은 목적 단백질을 세포 외로 고효율로 분비시킬 수 있는 시그널 펩타이드를 효율적으로 탐색하기 위해 시그널 펩타이드 암호화 서열을 용이하게 교체할 수 있는 발현 벡터를 제작하고, 이를 이용하여 800개의 시그널 펩타이드 암호화 서열이 삽입된 발현 벡터 라이브러리를 제작하였다. 목적 단백질을 암호화하는 유전자를 상기 라이브러리를 구성하는 각각의 발현 벡터에 도입하고, 숙주세포에서 발현시킨 후 숙주세포의 배양액을 이용하여 목적 단백질을 정량하면 목적 단백질을 외부로 분비시킬 수 있는 최적의 시그널 펩타이드를 선정할 수 있다.
본 발명의 목적은 목적 단백질을 세포 외로 분비시키기 위한 시그널 펩타이드 암호화 서열을 포함하는 발현 벡터를 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 5'에서 3' 방향으로 프로모터, 제1 제한효소 인식 부위, 코작 서열, 시그널 펩타이드 암호화 서열, 제2 제한효소 인식 부위 및 poly A 서열을 순차적으로 포함하는, 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터를 제공한다.
또한, 본 발명은 시그널 펩타이드 암호화 서열은 서열번호 1 내지 800으로 이루어진 군으로부터 선택되는 어느 하나로 기재되는 아미노산 서열을 암호화하는 서열인 목적 단백질을 세포 외로 분비시키기 위한 상기 벡터들을 포함하는, 발현 벡터 라이브러리를 제공한다.
또한, 본 발명은 1) 상기 라이브러리에 포함되는 2 내지 800 종류의 발현 벡터에 목적 단백질을 암호화하는 유전자를 삽입하는 단계; 2) 상기 단계 1)의 목적 단백질을 암호화하는 유전자가 삽입된 발현 벡터를 각각 숙주세포에 형질도입(transient transfection)하는 단계; 및 3) 상기 단계 2)의 형질도입된 숙주세포의 배양액으로부터 목적 단백질을 정량하는 단계를 포함하는, 목적 단백질을 세포 외로 대량으로 분비시킬 수 있는 시그널 펩타이드의 탐색 방법을 제공한다.
본 발명의 벡터 라이브러리 제조를 위한 발현 벡터는 시그널 펩타이드 암호화 서열 및 목적 단백질을 암호화하는 서열을 용이하게 삽입하고 교체할 수 있는 제한효소 인식 부위를 포함하고 있어, 단시간에 다양한 발현 벡터를 제조할 수 있다.
또한, 본 발명은 800개의 시그널 펩타이드 암호화 서열이 클로닝된 발현 벡터 라이브러리를 제공하며, 상기 발현 벡터 라이브러리를 이용하여 시그널 펩타이드 간의 목적 단백질의 세포외 분비량을 비교할 수 있으므로 목적 단백질에 적합한 시그널 펩타이드를 탐색하는 데에 유용하게 사용될 수 있다.
도 1은 본 발명의 발현 벡터를 제작하기 위한 모벡터인 pcDNA3.1(+)/V5-His B의 계열 지도이다.
도 2는 도 1의 모벡터로부터 제작된 pcDNA3.1(+)-Fluc-8XHis의 계열 지도이다.
도 3은 도 1의 모벡터로부터 제작된 pcDNA3.1(+)SPs-Fluc-8XHis의 계열 지도이다.
도 4는 본 발명의 일 실시예에 따라 제작된 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트(insert)의 모식도이다.
도 5는 본 발명의 일 실시예에 따라, 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트를 4개의 폴리뉴클레오티드로부터 제작하는 방법을 나타낸 것이다.
도 6은 본 발명의 일 실시예에 따라, 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트를 6개의 폴리뉴클레오티드로부터 제작하는 방법을 나타낸 것이다.
도 7은 서열번호 1 내지 50 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 8은 서열번호 51 내지 100 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 9는 서열번호 101 내지 150 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 10은 서열번호 151 내지 200 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 11은 서열번호 201 내지 250 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 12는 서열번호 251 내지 300 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 13은 서열번호 301 내지 350 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 14는 서열번호 351 내지 400 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 15는 서열번호 401 내지 450 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 16은 서열번호 451 내지 500 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 17은 서열번호 501 내지 550 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 18은 서열번호 551 내지 600 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 19는 서열번호 601 내지 650 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 20은 서열번호 651 내지 700 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 21는 서열번호 701 내지 750 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 22는 서열번호 751 내지 800 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 2는 도 1의 모벡터로부터 제작된 pcDNA3.1(+)-Fluc-8XHis의 계열 지도이다.
도 3은 도 1의 모벡터로부터 제작된 pcDNA3.1(+)SPs-Fluc-8XHis의 계열 지도이다.
도 4는 본 발명의 일 실시예에 따라 제작된 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트(insert)의 모식도이다.
도 5는 본 발명의 일 실시예에 따라, 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트를 4개의 폴리뉴클레오티드로부터 제작하는 방법을 나타낸 것이다.
도 6은 본 발명의 일 실시예에 따라, 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트를 6개의 폴리뉴클레오티드로부터 제작하는 방법을 나타낸 것이다.
도 7은 서열번호 1 내지 50 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 8은 서열번호 51 내지 100 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 9는 서열번호 101 내지 150 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 10은 서열번호 151 내지 200 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 11은 서열번호 201 내지 250 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 12는 서열번호 251 내지 300 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 13은 서열번호 301 내지 350 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 14는 서열번호 351 내지 400 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 15는 서열번호 401 내지 450 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 16은 서열번호 451 내지 500 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 17은 서열번호 501 내지 550 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 18은 서열번호 551 내지 600 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 19는 서열번호 601 내지 650 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 20은 서열번호 651 내지 700 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 21는 서열번호 701 내지 750 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
도 22는 서열번호 751 내지 800 각각의 시그널 펩타이드 암호화 서열과 조합되어 발현된 세포외 루시퍼라아제의 상대적 활성을 나타낸 것이다. CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타내는 것이다.
이하, 본 발명을 상세히 설명한다.
본 발명은 5'에서 3' 방향으로 프로모터, 제1 제한효소 인식 부위, 코작 서열, 시그널 펩타이드 암호화 서열, 제2 제한효소 인식 부위 및 poly A 서열을 순차적으로 포함하는 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터를 제공한다.
본 발명에 있어서, 본 발명에서 "발현 벡터"는 적합한 조절 서열에 작동가능하게 연결된 외부 DNA 서열을 포함하는 DNA 작제물로, 재조합 펩타이드 또는 단백질을 발현시키기 위한 벡터를 의미한다. 본 발명의 발현 벡터는 전사를 가능하게 하는 프로모터, 자기 복제가 이루어지도록 하는 복제 개시점, 목적하는 벡터를 선별하기 위한 항생물질의 선택마커 유전자 및 목적 단백질을 암호화하는 유전자를 삽입할 수 있는 제한효소 인식 부위가 최소한 필요하며, 벡터의 골격(backbone) 및 선택된 숙주에 따라 달라질 수 있다. 벡터 제작에 있어서 상기 최소한으로 필요한 유전자들은 당업자에게는 널리 알려져 있으며, 유전자 발현 조건 및 목적에 따라 용이하게 선택할 수 있다.
본 발명에서, "작동가능하게 연결된 (operably linked)"은 프로모터에서 전사가 개시되고 암호화 서열을 통해 종료암호로 진행하는 데 작용하도록 단편이 배열되는 것을 나타낸다. 재조합 벡터와의 작동적 연결은 당해 기술분야에서 잘 알려진 유전자 재조합 기술을 이용하여 제조할 수 있으며, 부위-특이적 DNA 절단 및 연결은 당해 기술분야에서 일반적으로 알려진 효소 등을 사용한다.
본 발명에 있어서, 본 발명에서 "프로모터"는 폴리머라제에 대한 결합 부위를 포함하고 프로모터 하위 유전자의 mRNA로의 전사 개시 활성을 가지는, 암호화 서열의 상류(upstream)의 비해독된 핵산 서열을 의미한다. 구체적으로, "프로모터"란, 전사개시점 (+1) 부터 20~30 염기에 대해 상류에 있고, 정확한 위치로부터 RNA 폴리머라아제에 전사를 개시시키는 기능을 담당하는 TATA 박스 또는 TATA 박스 유사의 영역이 포함되지만, 반드시 이들 영역의 전후에 한정되는 것은 아니고, 이 영역 이외에, 발현조절을 위해 RNA 폴리머라아제 이외의 단백질이 회합하기 위해 필요한 영역을 포함해도 된다.
상기 프로모터는 포유동물 세포의 게놈으로부터 유래된 프로모터 또는 포유동물 바이러스로부터 유래된 프로모터일 수 있다. 구체적으로, 메탈로티오닌 프로모터, 아데노바이러스 후기 프로모터, 백시니아 바이러스 7.5K 프로모터, SV40 프로모터, CMV 프로모터, HSV의 tk 프로모터 등일 수 있으며, 바람직하게는 CMV 프로모터일 수 있으나, 이에 한정되지 않는다.
상기 코작 서열(Kozak sequence)은 ATG의 인식 및 단백질 합성의 시작을 알리는 역할을 하는 서열로, 바람직하게는 GCCACC(서열번호 801) 또는 GCCGCC(서열번호 802)의 염기서열로 이루어진 것일 수 있다.
본 발명에서 "제한효소 인식 부위"은 제한효소가 인식하여 절단하는 특정 뉴클레오티드 서열을 의미한다.
상기 제1 제한효소 또는 제2 제한효소는 6개 이상의 염기서열을 인식하여 절단하는 효소인 것이 바람직하며, 8개의 염기서열을 인식하여 절단하는 효소인 것이 더 바람직하나, 이에 한정되지 않는다. 상기 제1 제한효소 또는 제2 제한효소는 인식 부위의 뉴클레오티드 서열 수가 많은 제한효소를 사용하여, 벡터 내에서 절단하고자 하는 위치에서만 절단될 확률을 높일 수 있다. 구체적으로 상기 제1 제한효소 또는 제2 제한효소는 MreⅠ, AscⅠ, MauBⅠ, SwaⅠ, AsiSⅠ, AbsⅠ, CciNⅠ, FseⅠ, MssⅠ, NotⅠ, PalAⅠ, RigⅠ, SbfⅠ, SdaⅠ, SfaAⅠ, SgfⅠ, SgsⅠ, SmiⅠ, SrfⅠ, Sse8387Ⅰ, XhoⅠ, AatⅡ, AgeⅠ, ApaⅠ, AseⅠ, Asp718Ⅰ, BamHⅠ, BclI, BmtI, BspDI, BspEI, BssSI, EagI, EcoRⅠ, EcoRⅤ, HindⅢ, KasⅠ, KpnⅠ, MfeⅠ, MluⅠ, MspCⅠ, NcoⅠ, NheⅠ, NsiⅠ, PaeR7Ⅰ, PciⅠ, PstⅠ, SacⅠ, SmaⅠ, SpeⅠ, XbaⅠ, XhoⅠ, XmaⅠ 등 일 수 있다. 바람직하게는 상기 제1 제한효소는 NotⅠ 또는 MreⅠ일 수 있고, 상기 제2 제한효소는 AscⅠ, MauBⅠ, SwaⅠ 및 XhoⅠ로 이루어진 군으로부터 선택되는 어느 하나 이상일 수 있다.
본 발명에 있어서, "시그널 펩타이드 암호화 서열"은 세포에서 발현되는 목적 단백질을 주변세포질(periplasm) 또는 세포 외부로 분비하기 위해 상기 목적 단백질이 세포 내막을 통과할 수 있도록 도와주는 서열을 의미한다.
본 발명에 있어서, "목적 단백질을 암호화하는 유전자"는 발현하고자 하는 외래 산물을 코딩하는 유전자로써, 재조합 단백질로 발현 가능한 모든 종류의 단백질을 암호화하는 유전자를 의미한다.
상기 시그널 펩타이드 암호화 서열은 제1 제한효소 인식 부위과 제2 제한효소 인식 부위 사이에 위치하여, 상기 제한효소 처리를 통해 상기 발현 벡터의 시그널 펩타이드 암호화 서열을 교체할 수 있다.
상기 목적 단백질을 암호화하는 유전자는 시그널 펩타이드 암호화 서열의 하류(downstream) 및 poly A 서열 사이에 도입되는 것일 수 있다. 또한 상기 목적 단백질을 암호화하는 유전자는 이종의 제2 제한효소 인식 부위 사이에 위치하여, 상기 제한효소 처리를 통해 상기 발현 벡터의 목적 단백질을 암호화하는 유전자를 교체할 수 있다.
상기 시그널 펩타이드 암호화 서열은 서열번호 1 내지 800으로 이루어진 군으로부터 선택되는 어느 하나로 기재되는 아미노산 서열을 암호화하는 것일 수 있다.
본 발명에 있어서, "poly A 서열"은 전사 시 RNA의 3' 말단에 폴리아데닐을 형성시키는 서열로, 당해 기술분야에서 잘 알려진 poly A 서열, 예를 들어 인간 성장 호르몬 폴리아데닐레이션 서열(poly A 서열), 소 성장 호르몬 폴리아데닐레이션 서열 또는 SV40 바이러스 폴리아데닐레이션 신호일 수 있다.
상기 발현 벡터는 서열번호 805로 이루어진 pcDNA3.1(+)-Fluc-8XHis 벡터의 NotⅠ 및 AscⅠ 제한효소 인식 부위 사이에 코작 서열 및 시그널 펩타이드 암호화 서열이 순차적으로 삽입된 것일 수 있다.
또한, 본 발명은 시그널 펩타이드 암호화 서열은 서열번호 1 내지 800으로 이루어진 군으로부터 선택되는 어느 하나로 기재되는 아미노산 서열을 암호화하는 서열인, 목적 단백질을 세포 외로 분비시키기 위한 상기 벡터들을 포함하는 발현 벡터 라이브러리를 제공한다.
또한, 본 발명은 1) 상기 라이브러리에 포함된 2 내지 800 종류의 발현 벡터에 목적 단백질을 암호화하는 유전자를 삽입하는 단계; 2) 상기 단계 1)의 목적 단백질을 암호화하는 유전자가 삽입된 발현 벡터를 각각 숙주세포에 형질도입(transient transfection)하는 단계; 및 3) 상기 단계 2)의 형질도입된 숙주세포의 배양액으로부터 목적 단백질을 정량하는 단계를 포함하는 목적 단백질을 세포 외로 대량으로 분비시킬 수 있는 시그널 펩타이드의 탐색 방법을 제공한다.
상기 방법에 있어서, 단계 1)의 목적 단백질을 암호화하는 유전자를 삽입하는 방법은 당해 업계의 당업자에게 알려진 방법으로 수행하는 것이 바람직하다. 구체적으로는 제한효소 인식 부위를 이용한 삽입 방법일 수 있다.
상기 방법에 있어서, 단계 2)의 숙주세포는 동물세포인 것이 바람직하나, 이에 한정되는 것은 아니다.
본 발명의 구체적인 실시예에서, 본 발명자들은 상기 800 종류의 시그널 펩타이드 암호화 서열 및 목적 단백질을 암호화하는 유전자로 루시퍼라아제 유전자가 클로닝된 벡터를 각각 숙주세포에 형질도입(trnasient transfection)하여 배양하고, 상기 배양액으로부터 세포 외로 분비된 루시퍼라아제의 양을 정량하였다(실험예 2, 도 7 내지 9 참조). 그 결과, 루시퍼라아제는 서열번호 605 또는 614의 시그널 펩타이드 암호화 서열과 조합 시, 세포 외로 다량 분비되는 것으로 확인되었다. 따라서, 본 발명에서 제공하는 벡터 라이브러리를 이용하는 경우 세포 외로 분비되어야 할 임의의 목적 단백질을 위한 최적의 시그널 펩타이드를 단시간에 용이하게 스크리닝할 수 있다.
이하, 본 발명을 하기 실시예에 의해 상세히 설명한다.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다.
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실시예
1> 시그널
펩타이드
암호화 서열의 삽입을 위한 발현 벡터 제작
시그널 펩타이드 암호화 서열의 삽입을 위한 발현 벡터를 제작하기 위하여, 모벡터 pcDNA 3.1(+)/V5-His B 플라스미드(도 1)를 이용하여, pcDNA3.1(+)-Fluc-8XHis (도 2) 벡터를 제작하였다. pcDNA3.1(+)-Fluc-8XHis 벡터의 제작 과정은 다음과 같다.
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실시예
1-1>
Asc
Ⅰ-
루시퍼라아제
(firefly
luciferase
)-8X His 태그-
MauB
Ⅰ의 서열 삽입
pcDNA3.1(+)-Fluc-8XHis 벡터의 목적 단백질로 루시퍼라아제를 선택하였다. 모벡터 pcDNA 3.1(+)/V5-His B 벡터(인비트로젠, 43-0037)에 AscⅠ 제한효소 인식 서열-루시퍼라아제(firefly luciferase) 암호화 서열-8X His 태그 암호화 서열-MauBⅠ 제한효소 인식 서열 순의 염기서열을 삽입하고자, 모벡터 내에 존재하는 제한효소 인식 부위인 NotⅠ 및 XhoⅠ의 제한효소 인식 서열을 양 말단에 포함하는 NotⅠ 제한효소 인식 서열-AscⅠ 제한효소 인식 서열-루시퍼라아제 암호화 서열-8X His 태그 암호화 서열-MauBⅠ 제한효소 인식 서열-XhoⅠ 제한효소 인식 서열 순의 염기서열을 (주)바이오니아에 의뢰하여 합성하였다.
합성된 NotⅠ 제한효소 인식 서열-AscⅠ 제한효소 인식 서열-루시퍼라아제 암호화 서열-8X His 태그 암호화 서열-MauBⅠ 제한효소 인식 서열-XhoⅠ 제한효소 인식 서열의 폴리뉴클레오티드를 제한효소 NotⅠ/XhoⅠ로 처리하고, 모벡터 pcDNA 3.1(+)/V5-His B 도 제한효소 NotⅠ/XhoⅠ로 처리한 후, 이를 혼합하고 리가아제(ligase, 엔지노믹스)를 첨가하여 4℃에서 16시간 동안 반응시켰다. 반응 후 E. coli DH10-beta에 형질전환하고, 앰피실린(ampicillin)이 함유된 플레이트에서 클론을 선별하였다. 선별된 클론으로부터 추출한 플라스미드는 제한효소 NotⅠ/XhoⅠ로 절단하여, AscⅠ 및 MauBⅠ 제한효소 인식 부위가 삽입되었음을 확인하였다.
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실시예
1-2>
GST
태그 서열 삽입
다음, GST 태그를 포함한 서열 암호화하는 염기 서열을 삽입하기 위해, GST를 암호화하는 DNA를 포함하고 있는 pGEX4T-1(GE 헬스케어, 27-1542-01) 벡터를 주형으로 PCR을 수행하였다. GST 태그 앞에 트롬빈 부위와 FLAG 태그를 암호화하는 부위가 포함되도록 PCR을 수행하였다. 표 1은 PCR 반응물의 조성, 표 2는 PCR에 사용한 프라이머 서열, 표 3은 PCR 실험 조건을 나타낸 것이다.
| GST DNA (pGEX4T-1) | 1 ㎕ |
| pfu DNA 중합효소 (엔지노믹스, 2.5 U/㎕) | 0.5 ㎕ |
| XhoⅠ-GST 정방향 프라이머 (10 pmole/㎕) | 2 ㎕ |
| ApaⅠ역방향 프라이머 (10 pmole/㎕) | 2 ㎕ |
| 10 x 버퍼(buffer) | 2 ㎕ |
| 2.5 mM dNTP 믹스 | 2 ㎕ |
| 증류수 | 10.5 ㎕ |
| 프라이머 | 서열번호 | 서열(5'→3') |
| XhoⅠ-GST 정방향 프라이머 | 803 | gttactctcgagctggttccgcgtggatctgactacaaggatgacgacgataagatgagatcccctatactaggt |
| ApaⅠ 역방향 프라이머 | 804 | attagacgggcccgggtcaggatctttttggaggatggtcgccacc |
| 온도 | 시간 | 사이클수 |
| 95℃ | 5분 | 1 |
| 95℃ | 30초 | 30 |
| 56℃ | 40초 | |
| 72℃ | 1분 | |
| 72℃ | 10분 | 1 |
| 4℃ |
pGEX4T-1 벡터의 PCR 산물과 실시예 1-1에서 제작한 pcDNA3.1(+)-Fluc-8XHis 벡터 각각을 XhoⅠ/ApaⅠ(엔지노믹스)으로 절단하고 정제하였다. 제한효소 처리된 pGEX4T-1 벡터의 PCR 산물과 제한효소 처리된 실시예 1-1의 벡터를 혼합하고 리가아제(ligase, 엔지노믹스)를 첨가하여 4℃에서 16시간 동안 반응시켰다. 반응 후, E. coli DH10-beta에 형질전환하고 앰피실린(ampicillin)이 함유된 플레이트에서 클론을 선별하였다. 선별된 클론으로부터 플라스미드를 추출하여 제한효소 XhoⅠ/ApaⅠ으로 절단하고, 시퀀싱을 수행하였다. XhoⅠ 및 AspⅠ 인식 서열에 GST 태그를 암호화하는 서열이 제대로 삽입되었음을 확인하였고, 그 결과 서열번호 805로 이루어진 시그널 펩타이드 암호화 서열의 삽입을 위한 발현 벡터(pcDNA3.1(+)-Fluc-8XHis)를 제작하였다(도 2).
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실시예
2> 시그널
펩타이드
암호화 서열을 포함하는 DNA 인서트(insert)의 합성
먼저, 800 종류의 시그널 펩타이드의 아미노산 서열에 기초하여(표 4), 이를 암호화할 수 있는 염기서열을 인간 코돈으로 최적화하였다.
5' 말단으로부터 인간 코돈으로 최적화된 시그널 펩타이드 암호화 서열을 순차적으로 포함하고, 센스 가닥의 5' 말단에 GGCC overhang과 안티센스 가닥의 5' 말단에 CGC overhang을 가진 DNA 인서트(도 4)를 하기와 같이 준비하였다.
상기 DNA 인서트는 DNA 인서트 크기에 따라 4개 또는 6개의 올리고뉴클레오티드를 합성하여 제작하였다.
도 5는 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트 및 이를 제조하기 위한 4개의 올리고뉴클레오티드를 모식화하여 나타낸 것이다. 센스 가닥 제조를 위해 5' 말단에 GGCCGC 서열을 포함하는 제1 올리고뉴클레오티드 및 3' 말단에 GG를 포함한 제3 올리고뉴클레오티드의 2개의 폴리뉴클레오티드 단편을 합성하였다. 또한, 안티센스 가닥 제조를 위해 5' 말단에 CGCGCC 서열을 포함하는 제4 올리고뉴클레오티드 및 3' 말단에 GC를 포함한 제2 올리고뉴클레오티드의 2개의 폴리뉴클레오티드 단편을 합성하였다.
제2 올리고뉴클레오티드는 제1 올리고뉴클레오티드의 5' 말단 서열인 5'-GGCC-3'를 제외한 모든 서열에 상보적인 서열을 포함하며, 추가적으로 제3 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 13 bp 이상의 뉴클레오티드 서열을 5'말단에 추가적으로 포함하도록 디자인하였다.
제3 올리고뉴클레오티드는 제4 올리고뉴클레오티드의 5' 말단 서열인 5'-CGC-3'를 제외한 모든 서열에 상보적인 서열을 포함하며, 추가적으로 제2 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 13 bp 이상의 뉴클레오티드 서열을 5' 말단에 추가적으로 포함하도록 디자인하였다.
도 6는 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트 및 이를 제조하기 위한 6개의 올리고뉴클레오티드를 모식화하여 나타낸 것이다. 센스 가닥 제조를 위해 5' 말단에 GG 서열을 포함하는 제1 올리고뉴클레오티드, 제3 올리고뉴클레오티드 및 3' 말단에 GG를 포함한 제5 올리고뉴클레오티드의 3개의 폴리뉴클레오티드 단편을 합성하였다. 또한, 안티센스 가닥 제조를 위해 5' 말단에 CGCG 서열을 포함하는 제6 올리고뉴클레오티드, 제4 올리고뉴클레오티드 및 3' 말단에 GC를 포함한 제2 올리고뉴클레오티드의 3개의 폴리뉴클레오티드 단편을 합성하였다.
제2 올리고뉴클레오티드는 제1 올리고뉴클레오티드의 5' 말단 서열인 5'-GGCC-3'를 제외한 모든 서열에 상보적인 서열을 포함하며, 추가적으로 제3 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 8 bp 이상의 뉴클레오티드 서열을 5'말단에 추가적으로 포함하도록 디자인하였다.
제3 올리고뉴클레오티드는 제5 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 8 bp 이상의 제4 올리고뉴클레오티드의 5' 말단 서열을 제외한 모든 제4 올리고뉴클레오티드 서열에 상보적인 서열을 포함하며, 추가적으로 제2 올리고뉴클레오티드의 5' 말단 서열에 상보적인 적어도 8 bp 이상의 뉴클레오티드 서열을 5'에 추가적으로 포함하도록 디자인하였다.
제4 올리고뉴클레오티드는 제2 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 8 bp 이상의 제3 올리고뉴클레오티드의 5' 말단 서열을 제외한 모든 제3 올리고뉴클레오티드 서열에 상보적인 서열을 포함하며, 추가적으로 제5 올리고뉴클레오티드의 5' 말단 서열에 상보적인 적어도 8 bp 이상의 뉴클레오티드 서열을 5'에 추가적으로 포함하도록 디자인하였다.
제5 올리고뉴클레오티드는 제6 올리고뉴클레오티드의 5' 말단 서열인 5'-GGCC-3'를 제외한 모든 서열에 상보적인 서열을 포함하며, 추가적으로 제4 올리고뉴클레오티드의 5' 말단 서열에 상보적인, 적어도 8 bp 이상의 뉴클레오티드 서열을 5'말단에 추가적으로 포함하도록 디자인하였다.
아래 표 5 및 표 6은 상기에 기재된 방법에 기초하여 일부 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트 제작을 위한 올리고뉴클레오티드 서열 세트를 나타낸 것이다. 나머지 모든 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트 제작을 위한 올리고뉴클레오티드 또한 시그널 펩타이드의 아미노산 서열에 기초하여 상기에 기재된 방법으로 준비하였다.
| 시그널 펩타이드 이름 |
시그널 펩타이드 서열번호 |
올리고뉴클레오티드 번호 |
서열번호 | 올리고뉴클레오티드 서열 (5'→3') |
| Uncharacterized protein KIAA0495 | 704 | 제1 올리고뉴클레오티드 | 806 | GGCCGCCGCCGGCGGCCACCATGTGCCTGC |
| 제2 올리고뉴클레오티드 | 807 | GGTCGCTGGCGGCGCTGCTGCTCAGCAGGCACATGGTGGCCGCCGGCGGC | ||
| 제3 올리고뉴클레오티드 | 808 | TGAGCAGCAGCGCCGCCAGCGACCTGGCCGCCACCAGCCTGACCGCCGGG | ||
| 제4 올리고뉴클레오티드 | 809 | CGCGCCCGGCGGTCAGGCTGGTGGCGGCCA | ||
| Interleukin-32 |
701 |
제1 올리고뉴클레오티드 | 810 | GGCCGCCGCCGGCGGCCACCATGTGCTTCCCCAAGGTGCTGAGCGACG |
| 제2 올리고뉴클레오티드 | 811 | GGGCCTTCAGCTTCTTCATGTCGTCGCTCAGCACCTTGGGGAAGCACATGGTGGCCGCCGGCGGC | ||
| 제3 올리고뉴클레오티드 | 812 | ACATGAAGAAGCTGAAGGCCCGCATGGTGATGCTGCTGCCCACCAGCGCCCAGGGCCTGGGCGGG | ||
| 제4 올리고뉴클레오티드 | 813 | ACATGAAGAAGCTGAAGGCCCGCATGGTGATGCTGCTGCCCACCAGCGCCCAGGGCCTGGGCGGG | ||
| Laminin subunit gamma-1 |
707 |
제1 올리고뉴클레오티드 | 814 | GGCCGCCGCCGGCGGCCACCATGCGCGGCAGCCACCGCGCCGCCCCC |
| 제2 올리고뉴클레오티드 | 815 | ACGGGCCACAGGCGGCCACGGGGGCGCAGGGCGGGGGCGGCGCGGTGGCTGCCGCGCATggtggccgccggcggc | ||
| 제3 올리고뉴클레오티드 | 816 | GCCCTGCGCCCCCGTGGCCGCCTGTGGCCCGTGCTGGCCGTGCTGGCCGCCGCCGCCGCCGCCGGCTGCGCCggg | ||
| 제4 올리고뉴클레오티드 | 817 | CGCGCCCGGCGCAGCCGGCGGCGGCGGCGGCGGCCAGCACGGCCAGC |
| 시그널 펩타이드 이름 |
시그널 펩타이드 서열번호 |
올리고뉴클레오티드 번호 |
서열번호 | 올리고뉴클레오티드 서열 (5'→3') |
| Interleukin-32 |
701 |
제1 올리고뉴클레오티드 | 818 | GGCCGCCGCCGGCGGCCACCATGTGCTTCCCCAAG |
| 제2 올리고뉴클레오티드 | 819 | GCTCAGCACCTTGGGGAAGCACATGGTGGCCGCCGGCGGC | ||
| 제3 올리고뉴클레오티드 | 820 | GTGCTGAGCGACGACATGAAGAAGCTGAAGGCCCGCATGGTGATG | ||
| 제4 올리고뉴클레오티드 | 821 | GGGCAGCAGCATCACCATGCGGGCCTTCAGCTTCTTCATGTCGTC | ||
| 제5 올리고뉴클레오티드 | 822 | CTGCTGCCCACCAGCGCCCAGGGCCTGGGCGGG | ||
| 제6 올리고뉴클레오티드 | 823 | CGCGCCCGCCCAGGCCCTGGGCGCTGGT | ||
| Laminin subunit gamma-1 |
707 |
제1 올리고뉴클레오티드 | 824 | GGCCGCCGCCGGCGGCCACCATGCGCGGCAGCCACCGCGC |
| 제2 올리고뉴클레오티드 | 825 | GCGGGGGCGGCGCGGTGGCTGCCGCGCATGGTGGCCGCCGGCGGC | ||
| 제3 올리고뉴클레오티드 | 826 | CGCCCCCGCCCTGCGCCCCCGTGGCCGCCTGT | ||
| 제4 올리고뉴클레오티드 | 827 | GCACGGGCCACAGGCGGCCACGGGGGCGCAGG | ||
| 제5 올리고뉴클레오티드 | 828 | GGCCCGTGCTGGCCGTGCTGGCCGCCGCCGCCGCCGCCGGCTGCGCCGGG | ||
| 제6 올리고뉴클레오티드 | 829 | CGCGCCCGGCGCAGCCGGCGGCGGCGGCGGCGGCCAGCACGGCCA |
시그널 펩타이드 암호화 서열을 포함하는 각각의 DNA 인서트는 4개 또는 6개의 올리고뉴클레오티드를 서로 어닐링하여 제조하였다. 어닐링 과정은 다음과 같다. 각 올리고뉴클레오티드 2 ㎍, 10X 어닐링 버퍼(100 mM Tris-HCl(pH 7.5-8.0), 10 mM EDTA(pH 8.0), 500 mM NaCl) 5 ㎕을 혼합하고, 증류수로 최종 부피 50 ㎕까지 맞춘 후 표 7과 같은 조건에서 반응시켜 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트를 수득하였다.
| 온도 | 시간 |
| 95℃ | 5분 |
| 90℃ | 2분 30초 |
| 85℃ | 2분 30초 |
| 80℃ | 2분 30초 |
| 75℃ | 2분 30초 |
| 70℃ | 2분 30초 |
| 65℃ | 2분 30초 |
| 60℃ | 2분 30초 |
| 55℃ | 2분 30초 |
| 50℃ | 2분 30초 |
| 45℃ | 2분 30초 |
| 40℃ | 2분 30초 |
| 35℃ | 2분 30초 |
| 30℃ | 2분 30초 |
| 25℃ | 30분 |
<
실시예
3> 시그널
펩타이드
암호화 서열을 포함하는 DNA 인서트의
클로닝
및 발현 벡터 라이브러리 제작
실시예 1에서 제작한 pcDNA3.1(+)-Fluc-8XHis 벡터를 제한효소 NotⅠ/AscⅠ(엔지노믹스)로 절단하고, DNA gel extraction을 통해 정제하였다.
실시예 2에서 제작한 시그널 펩타이드 암호화 서열을 포함하는 DNA 인서트 2 ㎕, 제한효소 처리된 실시예 1의 pcDNA3.1(+)-Fluc-8XHis 벡터 10 ng, 10X T4 DNA 리가아제 버퍼 2 ㎕, T4 DNA 리가아제 1 ㎕를 혼합하고, 증류수로 최종 부피 20 ㎕까지 맞춘 후, 4℃에서 16시간 동안 반응시켰다. 반응 완료 후, E. coli DH10-beta에 형질전환한 다음, 앰피실린이 함유된 플레이트에서 클론을 선별하였다. 선별된 클론으로부터 플라스미드 DNA를 추출한 후, DNA 시퀀싱을 수행하여 제대로 클로닝되었는지 확인하였다. 도 3은 모벡터 pcDNA3.1(+)/V5-HisB 로부터 제작된 시그널 펩타이드 암호화 서열을 포함하는 pcDNA3.1(+)SPs-Fluc-8XHis 벡터를 나타낸 것이다.
각각의 800개의 시그널 펩타이드 암호화 서열을 위와 같은 방법으로 실시예 1에서 제작한 벡터에 클로닝하여 800개의 발현 벡터 라이브러리를 제작하였다. 상기 라이브러리를 구성하는 벡터에는 목적 단백질로 루시퍼라아제가 삽입되어 있지만, 벡터 내에 존재하는 제한효소 인식 서열을 이용하여 루시퍼라아제를 다양한 목적 단백질로 치환할 수 있다.
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실험예
1> 시그널
펩타이드
종류에 따른 목적 단백질
세포외
분비량 평가
시그널 펩타이드에 따른 목적 단백질의 세포외 분비량을 루시퍼라아제를 참조 단백질로 하여 조사하였다.
실시예 3에서 제작한 800 종류의 시그널 펩타이드 암호화 서열이 클로닝된 벡터를 한국생명공학연구원에서 입수한 HEK 293E 세포주 8×105 cells/mL에 폴리에틸렌이민(polyethylenimine, PEI)를 이용하여 형질도입(trnasient transfection)하였다.
형질도입 후 FBS와 항생제가 없는 DMEM 배지로 교체하고, 4일 후 배양액을 수확하였다. 배양액 20 ㎕와 루시퍼라아제 어세이 약(ONE-GloTM Luciferase assay system, E6110, Promega) 20 ㎕를 흰색 96웰 플레이트에서 혼합한 후 상온에서 30분 동안 반응시켰다. 반응 완료 후 Luminoskan ascent(Thermo scientific)로 발광 정도를 측정하여, 시그널 펩타이드에 따른 루시퍼라아제 분비량을 확인하였다.
도 7 내지 21은 실시예 3의 각 벡터로 형질전환된 동물세포 배양액으로부터 얻은 루시퍼라아제의 발광 결과를 나타낸 것이다. 도 7 내지 21에서 CAT-H 뒤에 기재된 번호는 시그널 펩타이드 서열번호를 나타낸 것이다. 도 7 내지 21을 참조하면, CAT-H605 및 CAT-H614는 높은 루시퍼라아제 활성을 나타냈으며, 이는 가장 낮은 활성을 나타내는 시그널 펩타이드에 비해 약 650배 높은 것이다. 상기 루시퍼라아제 활성은 세포 외로 분비된 루시퍼라아제의 양에 상응하는 것으로 판단할 수 있다. 따라서 목적 단백질 루시퍼라아제는 서열번호 605 또는 614의 시그널 펩타이드 암호화 서열과 조합 시에 다량으로 세포 외로 분비됨을 알 수 있다.
본 발명의 발현 벡터는 제1 제한효소, 제2 제한효소 인식 부위를 가지므로 시그널 펩타이드와 목적 단백질의 암호화 서열을 간단하게 교체할 수 있는 시스템을 제공한다. 또한, 본 발명은 800개의 시그널 펩타이드 암호화 서열이 클로닝된 발현 벡터 라이브러리를 제공하며, 상기 발현 벡터 라이브러리를 이용하여 시그널 펩타이드에 따른 목적 단백질의 세포외 분비량을 비교할 수 있으므로 목적 단백질에 적합한 시그널 펩타이드를 탐색하는 데에 유용하게 사용될 수 있다.
<110> The Industry & Academic Cooperation In Chungnam National University
Osong Medical Innovation Foundation
<120> Expression vector library including DNA sequence encoding signal
peptide for extracellular secretion of target protein
<130> 2017P-09-021
<160> 829
<170> KoPatentIn 3.0
<210> 1
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Afamin
<400> 1
Met Lys Leu Leu Lys Leu Thr Gly Phe Ile Phe Phe Leu Phe Phe Leu
1 5 10 15
Thr Glu Ser Leu Thr
20
<210> 2
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alkaline phosphatase
<400> 2
Met Gln Gly Pro Trp Val Leu Leu Leu Leu Gly Leu Arg Leu Gln Leu
1 5 10 15
Ser Leu Gly
<210> 3
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-1-antitrypsin
<400> 3
Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys
1 5 10 15
Cys Leu Val Pro Val Ser Leu Ala
20
<210> 4
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-2-macroglobulin
<400> 4
Met Gly Lys Asn Lys Leu Leu His Pro Ser Leu Val Leu Leu Leu Leu
1 5 10 15
Val Leu Leu Pro Thr Asp Ala
20
<210> 5
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Angiogenin
<400> 5
Met Val Met Gly Leu Gly Val Leu Leu Leu Val Phe Val Leu Gly Leu
1 5 10 15
Gly Leu Thr Pro Pro Thr Leu Ala
20
<210> 6
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein E
<400> 6
Met Lys Val Leu Trp Ala Ala Leu Leu Val Thr Phe Leu Ala Gly Cys
1 5 10 15
Gln Ala
<210> 7
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Appetite-regulating hormone
<400> 7
Met Pro Ser Pro Gly Thr Val Cys Ser Leu Leu Leu Leu Gly Met Leu
1 5 10 15
Trp Leu Asp Leu Ala Met Ala
20
<210> 8
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Arylsulfatase A
<400> 8
Met Gly Ala Pro Arg Ser Leu Leu Leu Ala Leu Ala Ala Gly Leu Ala
1 5 10 15
Val Ala
<210> 9
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Azurocidin
<400> 9
Met Thr Arg Leu Thr Val Leu Ala Leu Leu Ala Gly Leu Leu Ala Ser
1 5 10 15
Ser Arg Ala Gly Ser Ser Pro Leu Leu Asp
20 25
<210> 10
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cadherin-2
<400> 10
Met Cys Arg Ile Ala Gly Ala Leu Arg Thr Leu Leu Pro Leu Leu Ala
1 5 10 15
Ala Leu Leu Gln Ala Ser Val Glu Ala
20 25
<210> 11
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Calcitonin
<400> 11
Met Gly Phe Gln Lys Phe Ser Pro Phe Leu Ala Leu Ser Ile Leu Val
1 5 10 15
Leu Leu Gln Ala Gly Ser Leu His Ala
20 25
<210> 12
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxypeptidase A1
<400> 12
Met Arg Gly Leu Leu Val Leu Ser Val Leu Leu Gly Ala Val Phe Gly
1 5 10 15
<210> 13
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cathepsin B
<400> 13
Met Lys Val Leu Trp Ala Ala Leu Leu Val Thr Phe Leu Ala Gly Cys
1 5 10 15
Gln Ala
<210> 14
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chromogranin-A
<400> 14
Met Arg Ser Ala Ala Val Leu Ala Leu Leu Leu Cys Ala Gly Gln Val
1 5 10 15
Thr Ala
<210> 15
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cortistatin
<400> 15
Met Pro Leu Ser Pro Gly Leu Leu Leu Leu Leu Leu Ser Gly Ala Thr
1 5 10 15
Ala Thr
<210> 16
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Elastin
<400> 16
Met Ala Gly Leu Thr Ala Ala Ala Pro Arg Pro Gly Val Leu Leu Leu
1 5 10 15
Leu Leu Ser Ile Leu His Pro Ser Arg Pro
20 25
<210> 17
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibroblast growth factor 4
<400> 17
Met Ser Gly Pro Gly Thr Ala Ala Val Ala Leu Leu Pro Ala Val Leu
1 5 10 15
Leu Ala Leu Leu Ala Pro Trp Ala Gly Arg Gly Gly Ala Ala
20 25 30
<210> 18
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibronectin
<400> 18
Met Leu Arg Gly Pro Gly Pro Gly Leu Leu Leu Leu Ala Val Gln Cys
1 5 10 15
Leu Gly Thr Ala Val Pro Ser Thr Gly Ala Ser Lys Ser Lys Arg
20 25 30
<210> 19
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibulin-1
<400> 19
Met Glu Arg Ala Ala Pro Ser Arg Arg Val Pro Leu Pro Leu Leu Leu
1 5 10 15
Leu Gly Gly Leu Ala Leu Leu Ala Ala Gly Val Asp Ala
20 25
<210> 20
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastric triacylglycerol lipase
<400> 20
Met Trp Leu Leu Leu Thr Met Ala Ser Leu Ile Ser Val Leu Gly Thr
1 5 10 15
Thr His Gly
<210> 21
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastrin
<400> 21
Met Gln Arg Leu Cys Val Tyr Val Leu Ile Phe Ala Leu Ala Leu Ala
1 5 10 15
Ala Phe Ser Glu Ala
20
<210> 22
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glucosylceramidase
<400> 22
Met Ala Gly Ser Leu Thr Gly Leu Leu Leu Leu Gln Ala Val Ser Trp
1 5 10 15
Ala Ser Gly
<210> 23
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide High IgG Fc receptor I
<400> 23
Met Trp Phe Leu Thr Thr Leu Leu Leu Trp Val Pro Val Asp Gly
1 5 10 15
<210> 24
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Histatin-3
<400> 24
Met Lys Phe Phe Val Phe Ala Leu Ile Leu Ala Leu Met Leu Ser Met
1 5 10 15
Thr Gly Ala
<210> 25
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Human Serum Albumin
<400> 25
Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala
1 5 10 15
Tyr Ser
<210> 26
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin
<400> 26
Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu
1 5 10 15
Trp Gly Pro Asp Pro Ala Ala Ala
20
<210> 27
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like growth factor 1
<400> 27
Met Gly Lys Ile Ser Ser Leu Pro Thr Gln Leu Phe Lys Cys Cys Phe
1 5 10 15
Cys Asp Phe Leu Lys
20
<210> 28
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interferon alpha-17
<400> 28
Met Ala Leu Ser Phe Ser Leu Leu Met Ala Val Leu Val Leu Ser Tyr
1 5 10 15
Lys Ser Ile Cys Ser Leu Gly
20
<210> 29
<211> 14
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-2
<400> 29
Met Gln Leu Leu Ser Cys Ile Ala Leu Ile Leu Ala Leu Val
1 5 10
<210> 30
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lactotransferrin
<400> 30
Met Lys Leu Val Phe Leu Val Leu Leu Phe Leu Gly Ala Leu Gly Leu
1 5 10 15
Cys Leu Ala
<210> 31
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Nephronectin
<400> 31
Met Asp Phe Leu Leu Ala Leu Val Leu Val Ser Ser Leu Tyr Leu Gln
1 5 10 15
Ala Ala Ala
<210> 32
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuropeptide B
<400> 32
Met Ala Arg Ser Ala Thr Leu Ala Ala Ala Ala Leu Ala Leu Cys Leu
1 5 10 15
Leu Leu Ala Pro Pro Gly Leu Ala
20
<210> 33
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurotrophin 4
<400> 33
Met Leu Pro Leu Pro Ser Cys Ser Leu Pro Ile Leu Leu Leu Phe Leu
1 5 10 15
Leu Pro Ser Val Pro Ile Glu Ser
20
<210> 34
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neutrophil defensin 1
<400> 34
Met Arg Thr Leu Ala Ile Leu Ala Ala Ile Leu Leu Val Ala Leu Gln
1 5 10 15
Ala Gln Ala
<210> 35
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Oxytocin
<400> 35
Met Ala Gly Ser Ser Leu Ala Cys Cys Leu Leu Gly Leu Leu Ala Leu
1 5 10 15
Thr Ser Ala
<210> 36
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pancreatic alpha amylase
<400> 36
Met Lys Phe Phe Leu Leu Leu Phe Thr Ile Gly Phe Cys Trp Ala
1 5 10 15
<210> 37
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pancreatic prohormone
<400> 37
Met Ala Ala Ala Arg Leu Cys Leu Ser Leu Leu Leu Leu Ser Thr Cys
1 5 10 15
Val Ala Leu Leu Leu Gln Pro Leu Leu Gly Ala Gln Gly
20 25
<210> 38
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pepsin A4
<400> 38
Met Lys Trp Leu Leu Leu Leu Gly Leu Val Ala Leu Ser Glu Cys
1 5 10 15
<210> 39
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proenkephalin-A
<400> 39
Met Ala Arg Phe Leu Thr Leu Cys Thr Trp Leu Leu Leu Leu Gly Pro
1 5 10 15
Gly Leu Leu Ala Thr Val Arg Ala
20
<210> 40
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Resistin
<400> 40
Met Lys Ala Leu Cys Leu Leu Leu Leu Pro Val Leu Gly Leu Leu Val
1 5 10 15
Ser Ser
<210> 41
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide slit3
<400> 41
Met Ala Pro Gly Trp Ala Gly Val Gly Ala Ala Val Arg Ala Arg Leu
1 5 10 15
Ala Leu Ala Leu Ala Leu Ala Ser Val Leu Ser Gly Pro Pro Ala Val
20 25 30
Ala
<210> 42
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide SOD3
<400> 42
Met Leu Ala Leu Leu Cys Ser Cys Leu Leu Leu Ala Ala Gly Ala Ser
1 5 10 15
Asp Ala
<210> 43
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Statherin
<400> 43
Met Lys Phe Leu Val Phe Ala Phe Ile Leu Ala Leu Met Val Ser Met
1 5 10 15
Ile Gly Ala
<210> 44
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Thromopoietin
<400> 44
Met Glu Leu Thr Glu Leu Leu Leu Val Val Met Leu Leu Leu Thr Ala
1 5 10 15
Arg Leu Thr Leu Ser
20
<210> 45
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tissue-type plasminogen activator
<400> 45
Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly
1 5 10 15
Ala Val Phe Val Ser Pro
20
<210> 46
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trypsinogen-2
<400> 46
Met Asn Leu Leu Leu Ile Leu Thr Phe Val Ala Ala Ala Val Ala
1 5 10 15
<210> 47
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tyrosinase
<400> 47
Met Leu Leu Ala Val Leu Tyr Cys Leu Leu Trp Ser Phe Gln Thr Ser
1 5 10 15
Ala Gly
<210> 48
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uteroglobin
<400> 48
Met Lys Leu Ala Val Thr Leu Thr Leu Val Thr Leu Ala Leu Cys Cys
1 5 10 15
Ser Ser Ala Ser Ala
20
<210> 49
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vascular endothelial growth factor A
<400> 49
Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu
1 5 10 15
Tyr Leu His His Ala Lys Trp Ser Gln Ala
20 25
<210> 50
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Wnt-1
<400> 50
Met Gly Leu Trp Ala Leu Leu Pro Gly Trp Val Ser Ala Thr Leu Leu
1 5 10 15
Leu Ala Leu Ala Ala Leu Pro Ala Ala Leu Ala
20 25
<210> 51
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acidic mammalian chitinase
<400> 51
Met Thr Lys Leu Ile Leu Leu Thr Gly Leu Val Leu Ile Leu Asn Leu
1 5 10 15
Gln Leu Gly Ser Ala
20
<210> 52
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acrosin
<400> 52
Met Val Glu Met Leu Pro Thr Ala Ile Leu Leu Val Leu Ala Val Ser
1 5 10 15
Val Val Ala
<210> 53
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acrosomal protein SP-10
<400> 53
Met Asn Arg Phe Leu Leu Leu Met Ser Leu Tyr Leu Leu Gly Ser Ala
1 5 10 15
Arg Gly Thr Ser Ser
20
<210> 54
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acyloxyacyl hydrolase
<400> 54
Met Gln Ser Pro Trp Lys Ile Leu Thr Val Ala Pro Leu Phe Leu Leu
1 5 10 15
Leu Ser Leu Gln Ser Ser Ala
20
<210> 55
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAM DEC1
<400> 55
Met Leu Arg Gly Ile Ser Gln Leu Pro Ala Val Ala Thr Met Ser Trp
1 5 10 15
Val Leu Leu Pro Val Leu Trp Leu Ile Val Gln Thr Gln Ala
20 25 30
<210> 56
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAMTS-like protein 1
<400> 56
Met Glu Cys Cys Arg Arg Ala Thr Pro Gly Thr Leu Leu Leu Phe Leu
1 5 10 15
Ala Phe Leu Leu Leu Ser Ser Arg Thr Ala Arg Ser
20 25
<210> 57
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Adipolin
<400> 57
Met Arg Arg Trp Ala Trp Ala Ala Val Val Val Leu Leu Gly Pro Gln
1 5 10 15
Leu Val Leu Leu
20
<210> 58
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Adiponectin
<400> 58
Met Leu Leu Leu Gly Ala Val Leu Leu Leu Leu Ala Leu Pro Gly His
1 5 10 15
Asp Gln
<210> 59
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Adrenomedullin
<400> 59
Met Lys Leu Val Ser Val Ala Leu Met Tyr Leu Gly Ser Leu Ala Phe
1 5 10 15
Leu Gly Ala Asp Thr
20
<210> 60
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Agouti-related protein
<400> 60
Met Leu Thr Ala Ala Val Leu Ser Cys Ala Leu Leu Leu Ala Leu Pro
1 5 10 15
Ala Thr Arg Gly
20
<210> 61
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Agouti-signaling protein
<400> 61
Met Asp Val Thr Arg Leu Leu Leu Ala Thr Leu Leu Val Phe Leu Cys
1 5 10 15
Phe Phe Thr Ala Asn Ser
20
<210> 62
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Agrin
<400> 62
Met Ala Gly Arg Ser His Pro Gly Pro Leu Arg Pro Leu Leu Pro Leu
1 5 10 15
Leu Val Val Ala Ala Cys Val Leu Pro Gly Ala Gly Gly
20 25
<210> 63
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-1B-glycoprotein
<400> 63
Met Ser Met Leu Val Val Phe Leu Leu Leu Trp Gly Val Thr Trp Gly
1 5 10 15
Pro Val Thr Glu Ala
20
<210> 64
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-2-macroglobulin-like protein 1
<400> 64
Met Trp Ala Gln Leu Leu Leu Gly Met Leu Ala Leu Ser Pro Ala Ile
1 5 10 15
Ala
<210> 65
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-fetoprotein
<400> 65
Met Lys Trp Val Glu Ser Ile Phe Leu Ile Phe Leu Leu Asn Phe Thr
1 5 10 15
Glu Ser
<210> 66
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-lactalbumin
<400> 66
Met Arg Phe Phe Val Pro Leu Phe Leu Val Gly Ile Leu Phe Pro Ala
1 5 10 15
Ile Leu Ala
<210> 67
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ameloblastin
<400> 67
Met Ser Ala Ser Lys Ile Pro Leu Phe Lys Met Lys Asp Leu Ile Leu
1 5 10 15
Ile Leu Cys Leu Leu Glu Met Ser Phe Ala
20 25
<210> 68
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Amelogenin
<400> 68
Met Gly Thr Trp Ile Leu Phe Ala Cys Leu Leu Gly Ala Ala Phe Ala
1 5 10 15
<210> 69
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Amelotin
<400> 69
Met Arg Ser Thr Ile Leu Leu Phe Cys Leu Leu Gly Ser Thr Arg Ser
1 5 10 15
<210> 70
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Amiloride-sensitive amine oxidase
<400> 70
Met Gly Arg Gly Thr Leu Ala Leu Gly Trp Ala Gly Ala Ala Leu Leu
1 5 10 15
Leu Leu Gln Met Leu Ala Ala Ala
20
<210> 71
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Amphiregulin
<400> 71
Met Arg Ala Pro Leu Leu Pro Pro Ala Pro Val Val Leu Ser Leu Leu
1 5 10 15
Ile Leu Gly
<210> 72
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Amyloid beta A4 protein
<400> 72
Met Leu Pro Gly Leu Ala Leu Leu Leu Leu Ala Ala Trp Thr Ala Arg
1 5 10 15
Ala
<210> 73
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Angiotensinogen
<400> 73
Met Arg Lys Arg Ala Pro Gln Ser Glu Met Ala Pro Ala Gly Val Ser
1 5 10 15
Leu Arg Ala Thr Ile Leu Cys Leu Leu Ala Trp Ala Gly Leu Ala Ala
20 25 30
Gly
<210> 74
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Anosmin-1
<400> 74
Met Val Pro Gly Val Pro Gly Ala Val Leu Thr Leu Cys Leu Trp Leu
1 5 10 15
Ala Ala Ser Ser Gly Cys Leu Ala
20
<210> 75
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Antileukoproteinase
<400> 75
Met Lys Ser Ser Gly Leu Phe Pro Phe Leu Val Leu Leu Ala Leu Gly
1 5 10 15
Thr Leu Ala Pro Trp Ala Val Glu Gly
20 25
<210> 76
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apelin
<400> 76
Met Asn Leu Arg Leu Cys Val Gln Ala Leu Leu Leu Leu Trp Leu Ser
1 5 10 15
Leu Thr Ala Val Cys Gly
20
<210> 77
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein A-I
<400> 77
Met Lys Ala Ala Val Leu Thr Leu Ala Val Leu Phe Leu Thr Gly Ser
1 5 10 15
Gln Ala
<210> 78
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein B-100
<400> 78
Met Asp Pro Pro Arg Pro Ala Leu Leu Ala Leu Leu Ala Leu Pro Ala
1 5 10 15
Leu Leu Leu Leu Leu Leu Ala Gly Ala Arg Ala
20 25
<210> 79
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Aspartylglucosaminidase
<400> 79
Met Ala Arg Lys Ser Asn Leu Pro Val Leu Leu Val Pro Phe Leu Leu
1 5 10 15
Cys Gln Ala Leu Val Arg Cys
20
<210> 80
<211> 14
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Asporin
<400> 80
Met Lys Glu Tyr Val Leu Leu Leu Phe Leu Ala Leu Cys Ser
1 5 10
<210> 81
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Augurin
<400> 81
Met Ala Ala Ser Pro Ala Arg Pro Ala Val Leu Ala Leu Thr Gly Leu
1 5 10 15
Ala Leu Leu Leu Leu Leu Cys Trp Gly Pro Gly Gly Ile Ser Gly
20 25 30
<210> 82
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Basigin
<400> 82
Met Ala Ala Ala Leu Phe Val Leu Leu Gly Phe Ala Leu Leu Gly Thr
1 5 10 15
His Gly Ala Ser Gly
20
<210> 83
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-2-glycoprotein 1
<400> 83
Met Ile Ser Pro Val Leu Ile Leu Phe Ser Ser Phe Leu Cys His Val
1 5 10 15
Ala Ile Ala
<210> 84
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-2-microglobulin
<400> 84
Met Ser Arg Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Leu Ser
1 5 10 15
Gly Leu Glu Ala
20
<210> 85
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-hexosaminidase subunit alpha
<400> 85
Met Thr Ser Ser Arg Leu Trp Phe Ser Leu Leu Leu Ala Ala Ala Phe
1 5 10 15
Ala Gly Arg Ala Thr Ala
20
<210> 86
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-microseminoprotein
<400> 86
Met Asn Val Leu Leu Gly Ser Val Val Ile Phe Ala Thr Phe Val Thr
1 5 10 15
Leu Cys Asn Ala
20
<210> 87
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-secretase 2
<400> 87
Met Gly Ala Leu Ala Arg Ala Leu Leu Leu Pro Leu Leu Ala Gln Trp
1 5 10 15
Leu Leu Arg Ala
20
<210> 88
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Biglycan
<400> 88
Met Trp Pro Leu Trp Arg Leu Val Ser Leu Leu Ala Leu Ser Gln Ala
1 5 10 15
<210> 89
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bone marrow proteoglycan
<400> 89
Met Lys Leu Pro Leu Leu Leu Ala Leu Leu Phe Gly Ala Val Ser Ala
1 5 10 15
<210> 90
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Brevican core protein
<400> 90
Met Ala Gln Leu Phe Leu Pro Leu Leu Ala Ala Leu Val Leu Ala Gln
1 5 10 15
Ala Pro Ala Ala Leu Ala
20
<210> 91
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Calreticulin
<400> 91
Met Leu Leu Ser Val Pro Leu Leu Leu Gly Leu Leu Gly Leu Ala Val
1 5 10 15
Ala
<210> 92
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Calsequestrin-2
<400> 92
Met Lys Arg Thr His Leu Phe Ile Val Gly Ile Tyr Phe Leu Ser Ser
1 5 10 15
Cys Arg Ala
<210> 93
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxylesterase 1
<400> 93
Met Trp Leu Arg Ala Phe Ile Leu Ala Thr Leu Ser Ala Ser Ala Ala
1 5 10 15
Trp
<210> 94
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cartilage intermediate layer protein 1
<400> 94
Met Val Gly Thr Lys Ala Trp Val Phe Ser Phe Leu Val Leu Glu Val
1 5 10 15
Thr Ser Val Leu Gly
20
<210> 95
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cerebellin-1
<400> 95
Met Leu Gly Val Leu Glu Leu Leu Leu Leu Gly Ala Ala Trp Leu Ala
1 5 10 15
Gly Pro Ala Arg Gly
20
<210> 96
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ceruloplasmin
<400> 96
Met Lys Ile Leu Ile Leu Gly Ile Phe Leu Phe Leu Cys Ser Thr Pro
1 5 10 15
Ala Trp Ala
<210> 97
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cholecystokinin
<400> 97
Met Asn Ser Gly Val Cys Leu Cys Val Leu Met Ala Val Leu Ala Ala
1 5 10 15
Gly Ala Leu Thr
20
<210> 98
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cholinesterase
<400> 98
Met His Ser Lys Val Thr Ile Ile Cys Ile Arg Phe Leu Phe Trp Phe
1 5 10 15
Leu Leu Leu Cys Met Leu Ile Gly Lys Ser His Thr
20 25
<210> 99
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chondroadherin-like protein
<400> 99
Met Glu Gly Pro Arg Ser Ser Thr His Val Pro Leu Val Leu Pro Leu
1 5 10 15
Leu Val Leu Leu Leu Leu Ala Pro Ala Arg Gln Ala Ala Ala
20 25 30
<210> 100
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chordin
<400> 100
Met Pro Ser Leu Pro Ala Pro Pro Ala Pro Leu Leu Leu Leu Gly Leu
1 5 10 15
Leu Leu Leu Gly Ser Arg Pro Ala Arg Gly
20 25
<210> 101
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Choriogonadotropin subunit beta 3
<400> 101
Met Glu Met Phe Gln Gly Leu Leu Leu Leu Leu Leu Leu Ser Met Gly
1 5 10 15
Gly Thr Trp Ala
20
<210> 102
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chorionic somatomammotropin hormone 2 isoform 2
<400> 102
Met Ala Ala Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Ala Leu Leu
1 5 10 15
Cys Leu Pro Trp Leu Gln Glu Ala Gly Ala
20 25
<210> 103
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chymase
<400> 103
Met Leu Leu Leu Pro Leu Pro Leu Leu Leu Phe Leu Leu Cys Ser Arg
1 5 10 15
Ala Glu Ala
<210> 104
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Clusterin
<400> 104
Met Met Lys Thr Leu Leu Leu Phe Val Gly Leu Leu Leu Thr Trp Glu
1 5 10 15
Ser Gly Gln Val Leu Gly
20
<210> 105
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Coagulation factor V
<400> 105
Met Phe Pro Gly Cys Pro Arg Leu Trp Val Leu Val Val Leu Gly Thr
1 5 10 15
Ser Trp Val Gly Trp Gly Ser Gln Gly Thr Glu Ala
20 25
<210> 106
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Colipase
<400> 106
Met Glu Lys Ile Leu Ile Leu Leu Leu Val Ala Leu Ser Val Ala Tyr
1 5 10 15
Ala
<210> 107
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-1(I) chain
<400> 107
Met Phe Ser Phe Val Asp Leu Arg Leu Leu Leu Leu Leu Ala Ala Thr
1 5 10 15
Ala Leu Leu Thr His Gly
20
<210> 108
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C3
<400> 108
Met Gly Pro Thr Ser Gly Pro Ser Leu Leu Leu Leu Leu Leu Thr His
1 5 10 15
Leu Pro Leu Ala Leu Gly
20
<210> 109
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Contactin-1
<400> 109
Met Lys Met Trp Leu Leu Val Ser His Leu Val Ile Ile Ser Ile Thr
1 5 10 15
Thr Cys Leu Ala
20
<210> 110
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Corneodesmosin
<400> 110
Met Gly Ser Ser Arg Ala Pro Trp Met Gly Arg Val Gly Gly His Gly
1 5 10 15
Met Met Ala Leu Leu Leu Ala Gly Leu Leu Leu Pro Gly Thr Leu Ala
20 25 30
<210> 111
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Corticoliberin
<400> 111
Met Arg Leu Pro Leu Leu Val Ser Ala Gly Val Leu Leu Val Ala Leu
1 5 10 15
Leu Pro Cys Pro Pro Cys Arg Ala
20
<210> 112
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Corticosteroid-binding globulin
<400> 112
Met Pro Leu Leu Leu Tyr Thr Cys Leu Leu Trp Leu Pro Thr Ser Gly
1 5 10 15
Leu Trp Thr Val Gln Ala
20
<210> 113
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Corticotropin-releasing factor-binding protein
<400> 113
Met Ser Pro Asn Phe Lys Leu Gln Cys His Phe Ile Leu Ile Phe Leu
1 5 10 15
Thr Ala Leu Arg Gly Glu Ser Arg
20
<210> 114
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-reactive protein
<400> 114
Met Glu Lys Leu Leu Cys Phe Leu Val Leu Thr Ser Leu Ser His Ala
1 5 10 15
Phe Gly
<210> 115
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cubilin
<400> 115
Met Met Asn Met Ser Leu Pro Phe Leu Trp Ser Leu Leu Thr Leu Leu
1 5 10 15
Ile Phe Ala Glu Val Asn Gly
20
<210> 116
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cystatin-C
<400> 116
Met Ala Gly Pro Leu Arg Ala Pro Leu Leu Leu Leu Ala Ile Leu Ala
1 5 10 15
Val Ala Leu Ala Val Ser Pro Ala Ala Gly
20 25
<210> 117
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Deoxyribonuclease-1
<400> 117
Met Arg Gly Met Lys Leu Leu Gly Ala Leu Leu Ala Leu Ala Ala Leu
1 5 10 15
Leu Gln Gly Ala Val Ser
20
<210> 118
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dermatopontin
<400> 118
Met Asp Leu Ser Leu Leu Trp Val Leu Leu Pro Leu Val Thr Met Ala
1 5 10 15
Trp Gly
<210> 119
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dermcidin
<400> 119
Met Arg Phe Met Thr Leu Leu Phe Leu Thr Ala Leu Ala Gly Ala Leu
1 5 10 15
Val Cys Ala
<210> 120
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dickkopf-related protein 1
<400> 120
Met Met Ala Leu Gly Ala Ala Gly Ala Thr Arg Val Phe Val Ala Met
1 5 10 15
Val Ala Ala Ala Leu Gly Gly His Pro Leu Leu Gly Val Ser Ala
20 25 30
<210> 121
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dystroglycan
<400> 121
Met Arg Met Ser Val Gly Leu Ser Leu Leu Leu Pro Leu Ser Gly Arg
1 5 10 15
Thr Phe Leu Leu Leu Leu Ser Val Val Met Ala Gln Ser
20 25
<210> 122
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EGF-like domain-containing protein 1
<400> 122
Met Gly Ala Ala Ala Val Arg Trp His Leu Cys Val Leu Leu Ala Leu
1 5 10 15
Gly Thr Arg Gly Arg Leu Ala
20
<210> 123
<211> 39
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Enamelin
<400> 123
Met Leu Val Leu Arg Cys Arg Leu Gly Thr Ser Phe Pro Lys Leu Asp
1 5 10 15
Asn Leu Val Pro Lys Gly Lys Met Lys Ile Leu Leu Val Phe Leu Gly
20 25 30
Leu Leu Gly Asn Ser Val Ala
35
<210> 124
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endothelin-1
<400> 124
Met Asp Tyr Leu Leu Met Ile Phe Ser Leu Leu Phe Val Ala Cys Gln
1 5 10 15
Gly
<210> 125
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endothelin-2
<400> 125
Met Val Ser Val Pro Thr Thr Trp Cys Ser Val Ala Leu Ala Leu Leu
1 5 10 15
Val Ala Leu His Glu Gly Lys Gly
20
<210> 126
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ephrin-A2
<400> 126
Met Ala Pro Ala Gln Arg Pro Leu Leu Pro Leu Leu Leu Leu Leu Leu
1 5 10 15
Pro Leu Pro Pro Pro Pro Phe Ala
20
<210> 127
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Epiphycan
<400> 127
Met Lys Thr Leu Ala Gly Leu Val Leu Gly Leu Val Ile Phe Asp Ala
1 5 10 15
Ala Val Thr
<210> 128
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Eppin
<400> 128
Met Gly Ser Ser Gly Leu Leu Ser Leu Leu Val Leu Phe Val Leu Leu
1 5 10 15
Ala Asn Val Gln Gly
20
<210> 129
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Erythroferrone
<400> 129
Met Ala Pro Ala Arg Arg Pro Ala Gly Ala Arg Leu Leu Leu Val Tyr
1 5 10 15
Ala Gly Leu Leu Ala Ala Ala Ala Ala Gly Leu Gly
20 25
<210> 130
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Erythropoietin
<400> 130
Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu
1 5 10 15
Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly
20 25
<210> 131
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide E-selectin
<400> 131
Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile
1 5 10 15
Lys Glu Ser Gly Ala
20
<210> 132
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibromodulin
<400> 132
Met Gln Trp Thr Ser Leu Leu Leu Leu Ala Gly Leu Phe Ser Leu Ser
1 5 10 15
Gln Ala
<210> 133
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Follicular dendritic cell secreted peptide
<400> 133
Met Lys Lys Val Leu Leu Leu Ile Thr Ala Ile Leu Ala Val Ala Val
1 5 10 15
Gly
<210> 134
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Follistatin
<400> 134
Met Val Arg Ala Arg His Gln Pro Gly Gly Leu Cys Leu Leu Leu Leu
1 5 10 15
Leu Leu Cys Gln Phe Met Glu Asp Arg Ser Ala Gln Ala
20 25
<210> 135
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Furin
<400> 135
Met Glu Leu Arg Pro Trp Leu Leu Trp Val Val Ala Ala Thr Gly Thr
1 5 10 15
Leu Val Leu Leu Ala Ala Asp Ala
20
<210> 136
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Galanin peptides
<400> 136
Met Ala Arg Gly Ser Ala Leu Leu Leu Ala Ser Leu Leu Leu Ala Ala
1 5 10 15
Ala Leu Ser
<210> 137
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Galanin-like peptide
<400> 137
Met Ala Pro Pro Ser Val Pro Leu Val Leu Leu Leu Val Leu Leu Leu
1 5 10 15
Ser Leu Ala Glu Thr Pro Ala Ser
20
<210> 138
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastrokine-1
<400> 138
Met Leu Ala Tyr Ser Ser Val His Cys Phe Arg Glu Asp Lys Met Lys
1 5 10 15
Phe Thr Ile Val Phe Ala Gly Leu Leu Gly Val Phe Leu Ala Pro Ala
20 25 30
Leu Ala
<210> 139
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glucagon
<400> 139
Met Lys Ser Ile Tyr Phe Val Ala Gly Leu Phe Val Met Leu Val Gln
1 5 10 15
Gly Ser Trp Gln
20
<210> 140
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glycophorin-A
<400> 140
Met Tyr Gly Lys Ile Ile Phe Val Leu Leu Leu Ser Glu Ile Val Ser
1 5 10 15
Ile Ser Ala
<210> 141
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glypican-1
<400> 141
Met Glu Leu Arg Ala Arg Gly Trp Trp Leu Leu Cys Ala Ala Ala Ala
1 5 10 15
Leu Val Ala Cys Ala Arg Gly
20
<210> 142
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide GPHB5 protein
<400> 142
Met Lys Leu Ala Phe Leu Phe Leu Gly Pro Met Ala Leu Leu Leu Leu
1 5 10 15
Ala Gly Tyr Gly Cys Val Leu Gly
20
<210> 143
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Granulysin
<400> 143
Met Ala Thr Trp Ala Leu Leu Leu Leu Ala Ala Met Leu Leu Gly Asn
1 5 10 15
Pro Gly Leu Val Phe Ser
20
<210> 144
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Granzyme B
<400> 144
Met Gln Pro Ile Leu Leu Leu Leu Ala Phe Leu Leu Leu Pro Arg Ala
1 5 10 15
Asp Ala
<210> 145
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Growth hormone variant
<400> 145
Met Ala Ala Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu
1 5 10 15
Cys Leu Ser Trp Leu Gln Glu Gly Ser Ala
20 25
<210> 146
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Guanylin
<400> 146
Met Asn Ala Phe Leu Leu Ser Ala Leu Cys Leu Leu Gly Ala Trp Ala
1 5 10 15
Ala Leu Ala Gly Gly
20
<210> 147
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Haptoglobin
<400> 147
Met Ser Ala Leu Gly Ala Val Ile Ala Leu Leu Leu Trp Gly Gln Leu
1 5 10 15
Phe Ala
<210> 148
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Heparanase
<400> 148
Met Leu Leu Arg Ser Lys Pro Ala Leu Pro Pro Pro Leu Met Leu Leu
1 5 10 15
Leu Leu Gly Pro Leu Gly Pro Leu Ser Pro Gly Ala Leu Pro Arg Pro
20 25 30
Ala Gln Ala
35
<210> 149
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hepatocyte growth factor
<400> 149
Met Trp Val Thr Lys Leu Leu Pro Ala Leu Leu Leu Gln His Val Leu
1 5 10 15
Leu His Leu Leu Leu Leu Pro Ile Ala Ile Pro Tyr Ala Glu Gly
20 25 30
<210> 150
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hepcidin
<400> 150
Met Ala Leu Ser Ser Gln Ile Trp Ala Ala Cys Leu Leu Leu Leu Leu
1 5 10 15
Leu Leu Ala Ser Leu Thr Ser Gly
20
<210> 151
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig heavy chain V-III region 23
<400> 151
Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly
1 5 10 15
Val Gln Cys
<210> 152
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig kappa chain V-III region HIC
<400> 152
Met Glu Thr Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro
1 5 10 15
Asp Thr Thr Gly
20
<210> 153
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like peptide INSL6
<400> 153
Met Pro Arg Leu Leu Arg Leu Ser Leu Leu Trp Leu Gly Leu Leu Leu
1 5 10 15
Val Arg Phe Ser
20
<210> 154
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Integrin alpha-2
<400> 154
Met Gly Pro Glu Arg Thr Gly Ala Ala Pro Leu Pro Leu Leu Leu Val
1 5 10 15
Leu Ala Leu Ser Gln Gly Ile Leu Asn Cys Cys Leu Ala
20 25
<210> 155
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Intelectin-1
<400> 155
Met Asn Gln Leu Ser Phe Leu Leu Phe Leu Ile Ala Thr Thr Arg Gly
1 5 10 15
Trp Ser
<210> 156
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interferon alpha-inducible protein 27-like protein
2
<400> 156
Met Met Lys Arg Ala Ala Ala Ala Ala Val Gly Gly Ala Leu Ala Val
1 5 10 15
Gly Ala Val Pro Val Val Leu Ser
20
<210> 157
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-2
<400> 157
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu
1 5 10 15
Val Thr Asn Ser
20
<210> 158
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-6
<400> 158
Met Asn Ser Phe Ser Thr Ser Ala Phe Gly Pro Val Ala Phe Ser Leu
1 5 10 15
Gly Leu Leu Leu Val Leu Pro Ala Ala Phe Pro Ala Pro
20 25
<210> 159
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-1
<400> 159
Met Trp Phe Leu Val Leu Cys Leu Ala Leu Ser Leu Gly Gly Thr Gly
1 5 10 15
Ala Ala
<210> 160
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-6
<400> 160
Met Lys Lys Leu Met Val Val Leu Ser Leu Ile Ala Ala Ala Trp Ala
1 5 10 15
<210> 161
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Keratocan
<400> 161
Met Ala Gly Thr Ile Cys Phe Ile Met Trp Val Leu Phe Ile Thr Asp
1 5 10 15
Thr Val Trp Ser
20
<210> 162
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kininogen-1
<400> 162
Met Lys Leu Ile Thr Ile Leu Phe Leu Cys Ser Arg Leu Leu Leu Ser
1 5 10 15
Leu Thr
<210> 163
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Klotho
<400> 163
Met Pro Ala Ser Ala Pro Pro Arg Arg Pro Arg Pro Pro Pro Pro Ser
1 5 10 15
Leu Ser Leu Leu Leu Val Leu Leu Gly Leu Gly Gly Arg Arg Leu Arg
20 25 30
Ala
<210> 164
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lactoperoxidase
<400> 164
Met Arg Val Leu Leu His Leu Pro Ala Leu Leu Ala Ser Leu Ile Leu
1 5 10 15
Leu Gln Ala Ala Ala Ser Thr Thr Arg Ala
20 25
<210> 165
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Legumain
<400> 165
Met Val Trp Lys Val Ala Val Phe Leu Ser Val Ala Leu Gly Ile Gly
1 5 10 15
Ala
<210> 166
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leptin
<400> 166
Met His Trp Gly Thr Leu Cys Gly Phe Leu Trp Leu Trp Pro Tyr Leu
1 5 10 15
Phe Tyr Val Gln Ala
20
<210> 167
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lumican
<400> 167
Met Ser Leu Ser Ala Phe Thr Leu Phe Leu Ala Leu Ile Gly Gly Thr
1 5 10 15
Ser Gly
<210> 168
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lymphotactin
<400> 168
Met Arg Leu Leu Ile Leu Ala Leu Leu Gly Ile Cys Ser Leu Thr Ala
1 5 10 15
Tyr Ile Val Glu Gly
20
<210> 169
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lysyl oxidase homolog 2
<400> 169
Met Glu Arg Pro Leu Cys Ser His Leu Cys Ser Cys Leu Ala Met Leu
1 5 10 15
Ala Leu Leu Ser Pro Leu Ser Leu Ala
20 25
<210> 170
<211> 37
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mammalian ependymin-related protein 1
<400> 170
Met Pro Gly Arg Ala Pro Leu Arg Thr Val Pro Gly Ala Leu Gly Ala
1 5 10 15
Trp Leu Leu Gly Gly Leu Trp Ala Trp Thr Leu Cys Gly Leu Cys Ser
20 25 30
Leu Gly Ala Val Gly
35
<210> 171
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mannose-binding protein C
<400> 171
Met Ser Leu Phe Pro Ser Leu Pro Leu Leu Leu Leu Ser Met Val Ala
1 5 10 15
Ala Ser Tyr Ser
20
<210> 172
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrilin-2
<400> 172
Met Glu Lys Met Leu Ala Gly Cys Phe Leu Leu Ile Leu Gly Gln Ile
1 5 10 15
Val Leu Leu Pro Ala Glu Ala
20
<210> 173
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Metalloproteinase inhibitor 2 (TIMP2)
<400> 173
Met Gly Ala Ala Ala Arg Thr Leu Arg Leu Ala Leu Gly Leu Leu Leu
1 5 10 15
Leu Ala Thr Leu Leu Arg Pro Ala Asp Ala
20 25
<210> 174
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Methyltransferase-like protein 7B
<400> 174
Met Asp Ile Leu Val Pro Leu Leu Gln Leu Leu Val Leu Leu Leu Thr
1 5 10 15
Leu Pro Leu His Leu Met Ala
20
<210> 175
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Midkine
<400> 175
Met Gln His Arg Gly Phe Leu Leu Leu Thr Leu Leu Ala Leu Leu Ala
1 5 10 15
Leu Thr Ser Ala
20
<210> 176
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mucin-5AC
<400> 176
Met Ser Val Gly Arg Arg Lys Leu Ala Leu Leu Trp Ala Leu Ala Leu
1 5 10 15
Ala Leu Ala Cys Thr Arg His Thr Gly His Ala
20 25
<210> 177
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Muellerian-inhibiting factor
<400> 177
Met Arg Asp Leu Pro Leu Thr Ser Leu Ala Leu Val Leu Ser Ala Leu
1 5 10 15
Gly Ala
<210> 178
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Multimerin-1
<400> 178
Met Lys Gly Ala Arg Leu Phe Val Leu Leu Ser Ser Leu Trp Ser Gly
1 5 10 15
Gly Ile Gly
<210> 179
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Myostatin
<400> 179
Met Gln Lys Leu Gln Leu Cys Val Tyr Ile Tyr Leu Phe Met Leu Ile
1 5 10 15
Val Ala
<210> 180
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Napsin-A
<400> 180
Met Ser Pro Pro Pro Leu Leu Gln Pro Leu Leu Leu Leu Leu Pro Leu
1 5 10 15
Leu Asn Val Glu Pro Ser Gly Ala Thr
20 25
<210> 181
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Natriuretic peptides A
<400> 181
Met Ser Ser Phe Ser Thr Thr Thr Val Ser Phe Leu Leu Leu Leu Ala
1 5 10 15
Phe Gln Leu Leu Gly Gln Thr Arg Ala
20 25
<210> 182
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Natriuretic peptides B
<400> 182
Met Asp Pro Gln Thr Ala Pro Ser Arg Ala Leu Leu Leu Leu Leu Phe
1 5 10 15
Leu His Leu Ala Phe Leu Gly Gly Arg Ser
20 25
<210> 183
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Netrin-4
<400> 183
Met Gly Ser Cys Ala Arg Leu Leu Leu Leu Trp Gly Cys Thr Val Val
1 5 10 15
Ala Ala
<210> 184
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neural cell adhesion molecule 1
<400> 184
Met Leu Gln Thr Lys Asp Leu Ile Trp Thr Leu Phe Phe Leu Gly Thr
1 5 10 15
Ala Val Ser
<210> 185
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurexophilin-2
<400> 185
Met Arg Leu Arg Pro Leu Pro Leu Val Val Val Pro Gly Leu Leu Gln
1 5 10 15
Leu Leu Phe Cys Asp Ser
20
<210> 186
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurocan core protein
<400> 186
Met Gly Ala Pro Phe Val Trp Ala Leu Gly Leu Leu Met Leu Gln Met
1 5 10 15
Leu Leu Phe Val Ala Gly
20
<210> 187
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuroendocrine convertase 1
<400> 187
Met Glu Arg Arg Ala Trp Ser Leu Gln Cys Thr Ala Phe Val Leu Phe
1 5 10 15
Cys Ala Trp Cys Ala Leu Asn Ser Ala Lys Ala
20 25
<210> 188
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuroendocrine convertase 2
<400> 188
Met Lys Gly Gly Cys Val Ser Gln Trp Lys Ala Ala Ala Gly Phe Leu
1 5 10 15
Phe Cys Val Met Val Phe Ala Ser Ala
20 25
<210> 189
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuromedin-B
<400> 189
Met Ala Arg Arg Ala Gly Gly Ala Arg Met Phe Gly Ser Leu Leu Leu
1 5 10 15
Phe Ala Leu Leu Ala Ala Gly Val
20
<210> 190
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurosecretory protein VGF
<400> 190
Met Lys Ala Leu Arg Leu Ser Ala Ser Ala Leu Phe Cys Leu Leu Leu
1 5 10 15
Ile Asn Gly Leu Gly Ala
20
<210> 191
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Niemann-Pick C1 protein
<400> 191
Met Thr Ala Arg Gly Leu Ala Leu Gly Leu Leu Leu Leu Leu Leu Cys
1 5 10 15
Pro Ala Gln Val Phe Ser
20
<210> 192
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Noggin
<400> 192
Met Glu Arg Cys Pro Ser Leu Gly Val Thr Leu Tyr Ala Leu Val Val
1 5 10 15
Val Leu Gly Leu Arg Ala Thr Pro Ala Gly Gly
20 25
<210> 193
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Norrin
<400> 193
Met Arg Lys His Val Leu Ala Ala Ser Phe Ser Met Leu Ser Leu Leu
1 5 10 15
Val Ile Met Gly Asp Thr Asp Ser
20
<210> 194
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Nyctalopin
<400> 194
Met Lys Gly Arg Gly Met Leu Val Leu Leu Leu His Ala Val Val Leu
1 5 10 15
Gly Leu Pro Ser Ala Trp Ala
20
<210> 195
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Olfactomedin-4
<400> 195
Met Arg Pro Gly Leu Ser Phe Leu Leu Ala Leu Leu Phe Phe Leu Gly
1 5 10 15
Gln Ala Ala Gly
20
<210> 196
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Osteoglycin
<400> 196
Met Lys Thr Leu Gln Ser Thr Leu Leu Leu Leu Leu Leu Val Pro Leu
1 5 10 15
Ile Lys Pro
<210> 197
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Otospiralin
<400> 197
Met Gln Ala Cys Met Val Pro Gly Leu Ala Leu Cys Leu Leu Leu Gly
1 5 10 15
Pro Leu Ala Gly Ala
20
<210> 198
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ovochymase-2
<400> 198
Met Leu Ile Ser Arg Asn Lys Leu Ile Leu Leu Leu Gly Ile Val Phe
1 5 10 15
Phe Glu Arg Gly Lys Ser
20
<210> 199
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Palmitoyl-protein thioesterase 1
<400> 199
Met Ala Ser Pro Gly Cys Leu Trp Leu Leu Ala Val Ala Leu Leu Pro
1 5 10 15
Trp Thr Cys Ala Ser Arg Ala Leu Gln His Leu
20 25
<210> 200
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pancreatic lipase-related protein 3
<400> 200
Met Leu Gly Ile Trp Ile Val Ala Phe Leu Phe Phe Gly Thr Ser Arg
1 5 10 15
Gly
<210> 201
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Parathyroid hormone
<400> 201
Met Ile Pro Ala Lys Asp Met Ala Lys Val Met Ile Val Met Leu Ala
1 5 10 15
Ile Cys Phe Leu Thr Lys Ser Asp Gly
20 25
<210> 202
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peptide YY
<400> 202
Met Val Phe Val Arg Arg Pro Trp Pro Ala Leu Thr Thr Val Leu Leu
1 5 10 15
Ala Leu Leu Val Cys Leu Gly Ala Leu Val Asp Ala
20 25
<210> 203
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Persephin
<400> 203
Met Ala Val Gly Lys Phe Leu Leu Gly Ser Leu Leu Leu Leu Ser Leu
1 5 10 15
Gln Leu Gly Gln Gly
20
<210> 204
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pikachurin
<400> 204
Met Asp Leu Ile Arg Gly Val Leu Leu Arg Leu Leu Leu Leu Ala Ser
1 5 10 15
Ser Leu Gly Pro Gly Ala Val Ser
20
<210> 205
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pituitary adenylate cyclase-activating polypeptide
<400> 205
Met Thr Met Cys Ser Gly Ala Arg Leu Ala Leu Leu Val Tyr Gly Ile
1 5 10 15
Ile Met His Ser Ser Val Tyr Ser
20
<210> 206
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Placenta-specific protein 1
<400> 206
Met Lys Val Phe Lys Phe Ile Gly Leu Met Ile Leu Leu Thr Ser Ala
1 5 10 15
Phe Ser Ala Gly Ser Gly
20
<210> 207
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasma serine protease inhibitor
<400> 207
Met Gln Leu Phe Leu Leu Leu Cys Leu Val Leu Leu Ser Pro Gln Gly
1 5 10 15
Ala Ser Leu
<210> 208
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Platelet-derived growth factor receptor beta
<400> 208
Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu
1 5 10 15
Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly
20 25 30
<210> 209
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prenylcysteine oxidase 1
<400> 209
Met Gly Arg Val Val Ala Glu Leu Val Ser Ser Leu Leu Gly Leu Trp
1 5 10 15
Leu Leu Leu Cys Ser Cys Gly Cys Pro Glu Gly
20 25
<210> 210
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prepronociceptin
<400> 210
Met Lys Val Leu Leu Cys Asp Leu Leu Leu Leu Ser Leu Phe Ser Ser
1 5 10 15
Val Phe Ser
<210> 211
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
<400> 211
Met Thr Ser Ser Gly Pro Gly Pro Arg Phe Leu Leu Leu Leu Pro Leu
1 5 10 15
Leu Leu Pro Pro Ala Ala Ser Ala
20
<210> 212
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proenkephalin-B
<400> 212
Met Ala Trp Gln Gly Leu Val Leu Ala Ala Cys Leu Leu Met Phe Pro
1 5 10 15
Ser Thr Thr Ala
20
<210> 213
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pro-epidermal growth factor
<400> 213
Met Leu Leu Thr Leu Ile Ile Leu Leu Pro Val Val Ser Lys Phe Ser
1 5 10 15
Phe Val Ser Leu Ser Ala
20
<210> 214
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proepiregulin
<400> 214
Met Thr Ala Gly Arg Arg Met Glu Met Leu Cys Ala Gly Arg Val Pro
1 5 10 15
Ala Leu Leu Leu Cys Leu Gly Phe His Leu Leu Gln Ala
20 25
<210> 215
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Progonadoliberin-1
<400> 215
Met Lys Pro Ile Gln Lys Leu Leu Ala Gly Leu Ile Leu Leu Thr Trp
1 5 10 15
Cys Val Glu Gly Cys Ser Ser
20
<210> 216
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prokineticin-1
<400> 216
Met Arg Gly Ala Thr Arg Val Ser Ile Met Leu Leu Leu Val Thr Val
1 5 10 15
Ser Asp Cys
<210> 217
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prokineticin-2
<400> 217
Met Arg Ser Leu Cys Cys Ala Pro Leu Leu Leu Leu Leu Leu Leu Pro
1 5 10 15
Pro Leu Leu Leu Thr Pro Arg Ala Gly Asp Ala
20 25
<210> 218
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolactin releasing hormone
<400> 218
Met Lys Val Leu Arg Ala Trp Leu Leu Cys Leu Leu Met Leu Gly Leu
1 5 10 15
Ala Leu Arg Gly Ala Ala Ser
20
<210> 219
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolactin-inducible protein
<400> 219
Met Arg Leu Leu Gln Leu Leu Phe Arg Ala Ser Pro Ala Thr Leu Leu
1 5 10 15
Leu Val Leu Cys Leu Gln Leu Gly Ala Asn Lys Ala
20 25
<210> 220
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolyl 3-hydroxylase 2
<400> 220
Met Arg Glu Arg Ile Trp Ala Pro Pro Leu Leu Leu Leu Leu Pro Leu
1 5 10 15
Leu Leu Pro Pro Pro Leu Trp Gly
20
<210> 221
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolyl 4-hydroxylase subunit alpha-1
<400> 221
Met Ile Trp Tyr Ile Leu Ile Ile Gly Ile Leu Leu Pro Gln Ser Leu
1 5 10 15
Ala
<210> 222
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Promotilin
<400> 222
Met Val Ser Arg Lys Ala Val Ala Ala Leu Leu Val Val His Val Ala
1 5 10 15
Ala Met Leu Ala Ser Gln Thr Glu Ala
20 25
<210> 223
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pro-neuropeptide Y
<400> 223
Met Leu Gly Asn Lys Arg Leu Gly Leu Ser Gly Leu Thr Leu Ala Leu
1 5 10 15
Ser Leu Leu Val Cys Leu Gly Ala Leu Ala Glu Ala
20 25
<210> 224
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pro-opiomelanocortin
<400> 224
Met Pro Arg Ser Cys Cys Ser Arg Ser Gly Ala Leu Leu Leu Ala Leu
1 5 10 15
Leu Leu Gln Ala Ser Met Glu Val Arg Gly
20 25
<210> 225
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prorelaxin H2
<400> 225
Met Pro Arg Leu Phe Phe Phe His Leu Leu Gly Val Cys Leu Leu Leu
1 5 10 15
Asn Gln Phe Ser Arg Ala Val Ala
20
<210> 226
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ProSAAS
<400> 226
Met Ala Gly Ser Pro Leu Leu Trp Gly Pro Arg Ala Gly Gly Val Gly
1 5 10 15
Leu Leu Val Leu Leu Leu Leu Gly Leu Phe Arg Pro Pro Pro Ala Leu
20 25 30
Cys
<210> 227
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prosalusin
<400> 227
Met Ala Ala Ala Thr Arg Gly Cys Arg Pro Trp Gly Ser Leu Leu Gly
1 5 10 15
Leu Leu Gly Leu Val Ser Ala Ala Ala Ala
20 25
<210> 228
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prostaglandin-H2 D-isomerase
<400> 228
Met Ala Thr His His Thr Leu Trp Met Gly Leu Ala Leu Leu Gly Val
1 5 10 15
Leu Gly Asp Leu Gln Ala
20
<210> 229
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prostate and testis expressed protein 2
<400> 229
Met Leu Val Leu Phe Leu Leu Gly Thr Val Phe Leu Leu Cys Pro Tyr
1 5 10 15
Trp Gly Glu Leu His Asp Pro Ile Lys Ala
20 25
<210> 230
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protachykinin-1
<400> 230
Met Lys Ile Leu Val Ala Leu Ala Val Phe Phe Leu Val Ser Thr Gln
1 5 10 15
Leu Phe Ala
<210> 231
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein disulfide-isomerase
<400> 231
Met Leu Arg Arg Ala Leu Leu Cys Leu Ala Val Ala Ala Leu Val Arg
1 5 10 15
Ala
<210> 232
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein O-glucosyltransferase 1
<400> 232
Met Glu Trp Trp Ala Ser Ser Pro Leu Arg Leu Trp Leu Leu Leu Phe
1 5 10 15
Leu Leu Pro Ser Ala Gln Gly
20
<210> 233
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prothrombin
<400> 233
Met Ala His Val Arg Gly Leu Gln Leu Pro Gly Cys Leu Ala Leu Ala
1 5 10 15
Ala Leu Cys Ser Leu Val His Ser
20
<210> 234
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pro-thyrotropin-releasing hormone
<400> 234
Met Pro Gly Pro Trp Leu Leu Leu Ala Leu Ala Leu Thr Leu Asn Leu
1 5 10 15
Thr Gly Val Pro Gly Gly Arg Ala
20
<210> 235
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pulmonary surfactant-associated protein D
<400> 235
Met Leu Leu Phe Leu Leu Ser Ala Leu Val Leu Leu Thr Gln Pro Leu
1 5 10 15
Gly Tyr Leu Glu
20
<210> 236
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Relaxin-3
<400> 236
Met Ala Arg Tyr Met Leu Leu Leu Leu Leu Ala Val Trp Val Leu Thr
1 5 10 15
Gly Glu Leu Trp Pro Gly Ala Glu Ala
20 25
<210> 237
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Renalase
<400> 237
Met Ala Gln Val Leu Ile Val Gly Ala Gly Met Thr Gly Ser Leu Cys
1 5 10 15
Ala
<210> 238
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Renin
<400> 238
Met Asp Gly Trp Arg Arg Met Pro Arg Trp Gly Leu Leu Leu Leu Leu
1 5 10 15
Trp Gly Ser Cys Thr Phe Gly
20
<210> 239
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Reticulocalbin-2
<400> 239
Met Arg Leu Gly Pro Arg Thr Ala Ala Leu Gly Leu Leu Leu Leu Cys
1 5 10 15
Ala Ala Ala Ala Gly Ala
20
<210> 240
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sarcalumenin
<400> 240
Met Arg Ala Leu Val Leu Leu Gly Cys Leu Leu Ala Ser Leu Leu Phe
1 5 10 15
Ser Gly Gln Ala
20
<210> 241
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sclerostin
<400> 241
Met Gln Leu Pro Leu Ala Leu Cys Leu Val Cys Leu Leu Val His Thr
1 5 10 15
Ala Phe Arg Val Val Glu Gly
20
<210> 242
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretin
<400> 242
Met Ala Pro Arg Pro Leu Leu Leu Leu Leu Leu Leu Leu Gly Gly Ser
1 5 10 15
Ala Ala
<210> 243
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretoglobin family 1D member 1
<400> 243
Met Arg Leu Ser Val Cys Leu Leu Leu Leu Thr Leu Ala Leu Cys Cys
1 5 10 15
Tyr Arg Ala Asn Ala
20
<210> 244
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretogranin-1
<400> 244
Met Gln Pro Thr Leu Leu Leu Ser Leu Leu Gly Ala Val Gly Leu Ala
1 5 10 15
Ala Val Asn Ser
20
<210> 245
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Selenoprotein M
<400> 245
Met Ser Leu Leu Leu Pro Pro Leu Ala Leu Leu Leu Leu Leu Ala Ala
1 5 10 15
Leu Val Ala Pro Ala Thr Ala
20
<210> 246
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Semenogelin-1
<400> 246
Met Lys Pro Asn Ile Ile Phe Val Leu Ser Leu Leu Leu Ile Leu Glu
1 5 10 15
Lys Gln Ala Ala Val Met Gly
20
<210> 247
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serglycin
<400> 247
Met Met Gln Lys Leu Leu Lys Cys Ser Arg Leu Val Leu Ala Leu Ala
1 5 10 15
Leu Ile Leu Val Leu Glu Ser Ser Val Gln Gly
20 25
<210> 248
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serotransferrin
<400> 248
Met Arg Leu Ala Val Gly Ala Leu Leu Val Cys Ala Val Leu Gly Leu
1 5 10 15
Cys Leu Ala
<210> 249
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serum amyloid A-1 protein
<400> 249
Met Lys Leu Leu Thr Gly Leu Val Phe Cys Ser Leu Val Leu Gly Val
1 5 10 15
Ser Ser
<210> 250
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serum amyloid P-component
<400> 250
Met Asn Lys Pro Leu Leu Trp Ile Ser Val Leu Thr Ser Leu Leu Glu
1 5 10 15
Ala Phe Ala
<210> 251
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Somatomedin-B and thrombospondin type-1
domain-containing protein
<400> 251
Met Arg Thr Leu Trp Met Ala Leu Cys Ala Leu Ser Arg Leu Trp Pro
1 5 10 15
Gly Ala Gln Ala
20
<210> 252
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Somatotropin
<400> 252
Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu
1 5 10 15
Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala
20 25
<210> 253
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sperm acrosome-associated protein 5
<400> 253
Met Lys Ala Trp Gly Thr Val Val Val Thr Leu Ala Thr Leu Met Val
1 5 10 15
Val Thr Val Asp Ala
20
<210> 254
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Spexin
<400> 254
Met Lys Gly Leu Arg Ser Leu Ala Ala Thr Thr Leu Ala Leu Phe Leu
1 5 10 15
Val Phe Val Phe Leu Gly Asn Ser Ser Cys
20 25
<210> 255
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Spondin-1
<400> 255
Met Arg Leu Ser Pro Ala Pro Leu Lys Leu Ser Arg Thr Pro Ala Leu
1 5 10 15
Leu Ala Leu Ala Leu Pro Leu Ala Ala Ala Leu Ala
20 25
<210> 256
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stanniocalcin-1
<400> 256
Met Leu Gln Asn Ser Ala Val Leu Leu Val Leu Val Ile Ser Ala Ser
1 5 10 15
Ala
<210> 257
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stereocilin
<400> 257
Met Ala Leu Ser Leu Trp Pro Leu Leu Leu Leu Leu Leu Leu Leu Leu
1 5 10 15
Leu Leu Ser Phe Ala Val
20
<210> 258
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Steryl-sulfatase
<400> 258
Met Pro Leu Arg Lys Met Lys Ile Pro Phe Leu Leu Leu Phe Phe Leu
1 5 10 15
Trp Glu Ala Glu Ser
20
<210> 259
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Submaxillary gland androgen-regulated protein 3A
<400> 259
Met Lys Ser Leu Thr Trp Ile Leu Gly Leu Trp Ala Leu Ala Ala Cys
1 5 10 15
Phe Thr Pro Gly Glu Ser
20
<210> 260
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sulfatase 1
<400> 260
Met Lys Tyr Ser Cys Cys Ala Leu Val Leu Ala Val Leu Gly Thr Glu
1 5 10 15
Leu Leu Gly Ser Leu Cys
20
<210> 261
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Syncollin
<400> 261
Met Ser Pro Leu Arg Pro Leu Leu Leu Ala Leu Ala Leu Ala Ser Val
1 5 10 15
Pro Cys Ala Gln Gly
20
<210> 262
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tachykinin-3
<400> 262
Met Arg Ile Met Leu Leu Phe Thr Ala Ile Leu Ala Phe Ser Leu Ala
1 5 10 15
<210> 263
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tenascin
<400> 263
Met Gly Ala Met Thr Gln Leu Leu Ala Gly Val Phe Leu Ala Phe Leu
1 5 10 15
Ala Leu Ala Thr Glu Gly
20
<210> 264
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Thyroglobulin
<400> 264
Met Ala Leu Val Leu Glu Ile Phe Thr Leu Leu Ala Ser Ile Cys Trp
1 5 10 15
Val Ser Ala
<210> 265
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Thyroid peroxidase
<400> 265
Met Arg Ala Leu Ala Val Leu Ser Val Thr Leu Val Met Ala Cys Thr
1 5 10 15
Glu Ala
<210> 266
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Transcobalamin-1
<400> 266
Met Arg Gln Ser His Gln Leu Pro Leu Val Gly Leu Leu Leu Phe Ser
1 5 10 15
Phe Ile Pro Ser Gln Leu Cys
20
<210> 267
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Transforming growth factor beta-1
<400> 267
Met Pro Pro Ser Gly Leu Arg Leu Leu Leu Leu Leu Leu Pro Leu Leu
1 5 10 15
Trp Leu Leu Val Leu Thr Pro Gly Arg Pro Ala Ala Gly
20 25
<210> 268
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Transthyretin
<400> 268
Met Ala Ser His Arg Leu Leu Leu Leu Cys Leu Ala Gly Leu Val Phe
1 5 10 15
Val Ser Glu Ala
20
<210> 269
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trehalase
<400> 269
Met Pro Gly Arg Thr Trp Glu Leu Cys Leu Leu Leu Leu Leu Gly Leu
1 5 10 15
Gly Leu Gly Ser Gln Glu Ala
20
<210> 270
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide TSHR protein
<400> 270
Met Arg Pro Ala Asp Leu Leu Gln Leu Val Leu Leu Leu Asp Leu Pro
1 5 10 15
Arg Asp Leu Gly Gly
20
<210> 271
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tsukushin
<400> 271
Met Pro Trp Pro Leu Leu Leu Leu Leu Ala Val Ser Gly Ala Gln Thr
1 5 10 15
<210> 272
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Urocortin
<400> 272
Met Arg Gln Ala Gly Arg Ala Ala Leu Leu Ala Ala Leu Leu Leu Leu
1 5 10 15
Val Gln Leu Cys Pro Gly Ser Ser Gln
20 25
<210> 273
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Urotensin-2B
<400> 273
Met Asn Lys Ile Leu Ser Ser Thr Val Cys Phe Gly Leu Leu Thr Leu
1 5 10 15
Leu Ser Val Leu Ser Phe Leu Gln Ser Val His Gly
20 25
<210> 274
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Versican core protein
<400> 274
Met Phe Ile Asn Ile Lys Ser Ile Leu Trp Met Cys Ser Thr Leu Ile
1 5 10 15
Val Thr His Ala
20
<210> 275
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vitrin
<400> 275
Met Arg Thr Val Val Leu Thr Met Lys Ala Ser Val Ile Glu Met Phe
1 5 10 15
Leu Val Leu Leu Val Thr Gly Val His Ser
20 25
<210> 276
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vitronectin
<400> 276
Met Ala Pro Leu Arg Pro Leu Leu Ile Leu Ala Leu Leu Ala Trp Val
1 5 10 15
Ala Leu Ala
<210> 277
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP four-disulfide core domain protein 2
<400> 277
Met Pro Ala Cys Arg Leu Gly Pro Leu Ala Ala Ala Leu Leu Leu Ser
1 5 10 15
Leu Leu Leu Phe Gly Phe Thr Leu Val Ser Gly Thr Gly Ala
20 25 30
<210> 278
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Zymogen granule membrane protein 16
<400> 278
Met Leu Thr Val Ala Leu Leu Ala Leu Leu Cys Ala Ser Ala Ser Gly
1 5 10 15
<210> 279
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cystatin-S
<400> 279
Met Ala Arg Pro Leu Cys Thr Leu Leu Leu Leu Met Ala Thr Leu Ala
1 5 10 15
Gly Ala Leu Ala
20
<210> 280
<211> 49
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase1
<400> 280
Met Gln Arg Ala Val Pro Glu Gly Phe Gly Arg Arg Lys Leu Gly Ser
1 5 10 15
Asp Met Gly Asn Ala Glu Arg Ala Pro Gly Ser Arg Ser Phe Gly Pro
20 25 30
Val Pro Thr Leu Leu Leu Leu Ala Ala Ala Leu Leu Ala Val Ser Asp
35 40 45
Ala
<210> 281
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leucine-rich alpha-2-glycoprotein
<400> 281
Met Ser Ser Trp Ser Arg Gln Arg Pro Lys Ser Pro Gly Gly Ile Gln
1 5 10 15
Pro His Val Ser Arg Thr Leu Phe Leu Leu Leu Leu Leu Ala Ala Ser
20 25 30
Ala Trp Gly
35
<210> 282
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bone morphogenetic protein 2
<400> 282
Met Val Ala Gly Thr Arg Cys Leu Leu Ala Leu Leu Leu Pro Gln Val
1 5 10 15
Leu Leu Gly Gly Ala Ala Gly
20
<210> 283
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bone morphogenetic protein 7
<400> 283
Met His Val Arg Ser Leu Arg Ala Ala Ala Pro His Ser Phe Val Ala
1 5 10 15
Leu Trp Ala Pro Leu Phe Leu Leu Arg Ser Ala Leu Ala
20 25
<210> 284
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q tumor necrosis factor-related
protein 8
<400> 284
Met Ala Ala Pro Ala Leu Leu Leu Leu Ala Leu Leu Leu Pro Val Gly
1 5 10 15
Ala
<210> 285
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxypeptidase E
<400> 285
Met Ala Gly Arg Gly Gly Ser Ala Leu Leu Ala Leu Cys Gly Ala Leu
1 5 10 15
Ala Ala Cys Gly Trp Leu Leu Gly Ala
20 25
<210> 286
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cholesteryl ester transfer protein
<400> 286
Met Leu Ala Ala Thr Val Leu Thr Leu Ala Leu Leu Gly Asn Ala His
1 5 10 15
Ala
<210> 287
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement factor I
<400> 287
Met Lys Leu Leu His Val Phe Leu Leu Phe Leu Cys Phe His Leu Arg
1 5 10 15
Phe Cys
<210> 288
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-3(VI) chain
<400> 288
Met Arg Lys His Arg His Leu Pro Leu Val Ala Val Phe Cys Leu Phe
1 5 10 15
Leu Ser Gly Phe Pro Thr Thr His Ala
20 25
<210> 289
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cysteine-rich motor neuron 1 protein
<400> 289
Met Tyr Leu Val Ala Gly Asp Arg Gly Leu Ala Gly Cys Gly His Leu
1 5 10 15
Leu Val Ser Leu Leu Gly Leu Leu Leu Leu Leu Ala Arg Ser Gly Thr
20 25 30
Arg Ala
<210> 290
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide UPF0672 protein CXorf36
<400> 290
Met Glu Pro Gln Leu Gly Pro Glu Ala Ala Ala Leu Arg Pro Gly Trp
1 5 10 15
Leu Ala Leu Leu Leu Trp Val Ser Ala Leu Ser Cys Ser Phe Ser
20 25 30
<210> 291
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cystatin-D
<400> 291
Met Met Trp Pro Met His Thr Pro Leu Leu Leu Leu Thr Ala Leu Met
1 5 10 15
Val Ala Val Ala
20
<210> 292
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 109
<400> 292
Met Arg Leu His Leu Leu Leu Leu Ile Leu Leu Leu Phe Ser Ile Leu
1 5 10 15
Leu Ser Pro Val Arg Gly
20
<210> 293
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dickkopf-related protein 2
<400> 293
Met Ala Ala Leu Met Arg Ser Lys Asp Ser Ser Cys Cys Leu Leu Leu
1 5 10 15
Leu Ala Ala Val Leu Met Val Glu Ser Ser Gln Ile Gly Ser Ser Arg
20 25 30
Ala
<210> 294
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EMILIN-1
<400> 294
Met Ala Pro Arg Thr Leu Trp Ser Cys Tyr Leu Cys Cys Leu Leu Thr
1 5 10 15
Ala Ala Ala Gly Ala
20
<210> 295
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibulin-2
<400> 295
Met Val Leu Leu Trp Glu Pro Ala Gly Ala Trp Leu Ala Leu Gly Leu
1 5 10 15
Ala Leu Ala Leu Gly Pro Ser Val Ala Ala Ala
20 25
<210> 296
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide FRAS1-related extracellular matrix protein 3
<400> 296
Met Ala Gly Ala Ser Arg His Pro Thr Gly Thr Pro Arg Gln Leu Leu
1 5 10 15
Val Ala Leu Ala Cys Leu Leu Leu Ser Arg Pro Ala Leu Gln
20 25 30
<210> 297
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gelsolin
<400> 297
Met Ala Pro His Arg Pro Ala Pro Ala Leu Leu Cys Ala Leu Ser Leu
1 5 10 15
Ala Leu Cys Ala Leu Ser Leu Pro Val Arg Ala
20 25
<210> 298
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-galactosidase-1-like protein
<400> 298
Met Ala Pro Lys Lys Leu Ser Cys Leu Arg Ser Leu Leu Leu Pro Leu
1 5 10 15
Ser Leu Thr Leu Leu Leu Pro Gln Ala Asp Thr
20 25
<210> 299
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasma protease C1 inhibitor
<400> 299
Met Ala Ser Arg Leu Thr Leu Leu Thr Leu Leu Leu Leu Leu Leu Ala
1 5 10 15
Gly Asp Arg Ala Ser Ser
20
<210> 300
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-10
<400> 300
Met His Ser Ser Ala Leu Leu Cys Cys Leu Val Leu Leu Thr Gly Val
1 5 10 15
Arg Ala
<210> 301
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-12 subunit alpha
<400> 301
Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val Leu Leu
1 5 10 15
Asp His Leu Ser Leu Ala
20
<210> 302
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-31
<400> 302
Met Ala Ser His Ser Gly Pro Ser Thr Ser Val Leu Phe Leu Phe Cys
1 5 10 15
Cys Leu Gly Gly Trp Leu Ala
20
<210> 303
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-1 receptor-like 1
<400> 303
Met Gly Phe Trp Ile Leu Ala Ile Leu Thr Ile Leu Met Tyr Ser Thr
1 5 10 15
Ala Ala
<210> 304
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Early placenta insulin-like peptide
<400> 304
Met Ala Ser Leu Phe Arg Ser Tyr Leu Pro Ala Ile Trp Leu Leu Leu
1 5 10 15
Ser Gln Leu Leu Arg Glu Ser Leu Ala
20 25
<210> 305
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-14
<400> 305
Met Ser Leu Arg Val Leu Gly Ser Gly Thr Trp Pro Ser Ala Pro Lys
1 5 10 15
Met Phe
<210> 306
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Extracellular glycoprotein lacritin
<400> 306
Met Lys Phe Thr Thr Leu Leu Phe Leu Ala Ala Val Ala Gly Ala Leu
1 5 10 15
Val Tyr Ala
<210> 307
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Left-right determination factor 1
<400> 307
Met Gln Pro Leu Trp Leu Cys Trp Ala Leu Trp Val Leu Pro Leu Ala
1 5 10 15
Ser Pro Gly Ala Ala
20
<210> 308
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lysyl oxidase homolog 3
<400> 308
Met Arg Pro Val Ser Val Trp Gln Trp Ser Pro Trp Gly Leu Leu Leu
1 5 10 15
Cys Leu Leu Cys Ser Ser Cys Leu Gly
20 25
<210> 309
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide 1-O-acylceramide synthase
<400> 309
Met Gly Leu His Leu Arg Pro Tyr Arg Val Gly Leu Leu Pro Asp Gly
1 5 10 15
Leu Leu Phe Leu Leu Leu Leu Leu Met Leu Leu Ala Asp Pro Ala Leu
20 25 30
Pro
<210> 310
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrilin-4
<400> 310
Met Arg Gly Leu Leu Cys Trp Pro Val Leu Leu Leu Leu Leu Gln Pro
1 5 10 15
Trp Glu
<210> 311
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrix metalloproteinase-26
<400> 311
Met Gln Leu Val Ile Leu Arg Val Thr Ile Phe Leu Pro Trp Cys Phe
1 5 10 15
Ala
<210> 312
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neutrophil collagenase
<400> 312
Met Phe Ser Leu Lys Thr Leu Pro Phe Leu Leu Leu Leu His Val Gln
1 5 10 15
Ile Ser Lys Ala
20
<210> 313
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Netrin-2-like protein
<400> 313
Met Pro Gly Trp Pro Trp Gly Leu Leu Leu Thr Ala Gly Thr Leu Phe
1 5 10 15
Ala Ala Leu Ser Pro Gly Pro Pro Ala Pro Ala
20 25
<210> 314
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuropeptide S
<400> 314
Met Ile Ser Ser Val Lys Leu Asn Leu Ile Leu Val Leu Ser Leu Ser
1 5 10 15
Thr Met His Val Phe Trp Cys
20
<210> 315
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proprotein convertase subtilisin/kexin type 5
<400> 315
Met Gly Trp Gly Ser Arg Cys Cys Cys Pro Gly Arg Leu Asp Leu Leu
1 5 10 15
Cys Val Leu Ala Leu Leu Gly Gly Cys Leu Leu Pro Val Cys Arg Thr
20 25 30
<210> 316
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Parathyroid hormone-related protein
<400> 316
Met Gln Arg Arg Leu Val Gln Gln Trp Ser Val Ala Val Phe Leu Leu
1 5 10 15
Ser Tyr Ala Val Pro Ser Cys Gly
20
<210> 317
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peroxidasin-like protein
<400> 317
Met Glu Pro Arg Leu Phe Cys Trp Thr Thr Leu Phe Leu Leu Ala Gly
1 5 10 15
Trp Cys Leu Pro Gly Leu Pro
20
<210> 318
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sulfhydryl oxidase 2
<400> 318
Met Ala Ala Ala Gly Ala Ala Val Ala Arg Ser Pro Gly Ile Gly Ala
1 5 10 15
Gly Pro Ala Leu Arg
20
<210> 319
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proactivator polypeptide
<400> 319
Met Tyr Ala Leu Phe Leu Leu Ala Ser Leu Leu Gly Ala Ala Leu Ala
1 5 10 15
<210> 320
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pulmonary surfactant-associated protein A1
<400> 320
Met Trp Leu Cys Pro Leu Ala Leu Asn Leu Ile Leu Met Ala Ala Ser
1 5 10 15
Gly Ala Val Cys
20
<210> 321
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tectonic-1
<400> 321
Met Arg Pro Arg Gly Leu Pro Pro Leu Leu Val Val Leu Leu Gly Cys
1 5 10 15
Trp Ala Ser Val Ser Ala
20
<210> 322
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein TMEM155
<400> 322
Met Ala Ser Asp Leu Ile Arg Thr Ile Leu Ala Val Ala Leu Ile Ser
1 5 10 15
Lys Leu Gly Thr Ala Val Asp Ala
20
<210> 323
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Twisted gastrulation protein homolog 1
<400> 323
Met Lys Leu His Tyr Val Ala Val Leu Thr Leu Ala Ile Leu Met Phe
1 5 10 15
Leu Thr Trp Leu Pro Glu Ser Leu Ser
20 25
<210> 324
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Urokinase plasminogen activator surface receptor
<400> 324
Met Gly His Pro Pro Leu Leu Pro Leu Leu Leu Leu Leu His Thr Cys
1 5 10 15
Val Pro Ala Ser Trp Gly
20
<210> 325
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide VIP peptides
<400> 325
Met Asp Thr Arg Asn Lys Ala Gln Leu Leu Val Leu Leu Thr Leu Leu
1 5 10 15
Ser Val Leu Phe
20
<210> 326
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Von Willebrand factor D and EGF domain-containing
protein
<400> 326
Met Pro Gly Gly Ala Cys Val Leu Val Ile Ala Leu Met Phe Leu Ala
1 5 10 15
Trp Gly Glu Ala
20
<210> 327
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP four-disulfide core domain protein 6
<400> 327
Met Gly Leu Ser Gly Leu Leu Pro Ile Leu Val Pro Phe Ile Leu Leu
1 5 10 15
Gly Asp Ile Gln Glu Pro Gly His Ala
20 25
<210> 328
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-8a
<400> 328
Met Gly Asn Leu Phe Met Leu Trp Ala Ala Leu Gly Ile Cys Cys Ala
1 5 10 15
Ala Phe Ser
<210> 329
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein FLJ46089
<400> 329
Met Arg Cys Val Thr Arg Thr Arg Asn Trp Trp Arg Arg Ala Ala Arg
1 5 10 15
Met Pro Arg Ala Gly Ser Ser Ala Trp Trp Val Ala Val Cys Lys Gln
20 25 30
Val Cys Thr
35
<210> 330
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide HLA class I histocompatibility antigen, Cw-16
alpha chain
<400> 330
Met Arg Val Met Ala Pro Arg Thr Leu Ile Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Glu Thr Trp Ala
20
<210> 331
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acrosin-binding protein
<400> 331
Met Arg Lys Pro Ala Ala Gly Phe Leu Pro Ser Leu Leu Lys Val Leu
1 5 10 15
Leu Leu Pro Leu Ala Pro Ala Ala Ala
20 25
<210> 332
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Angiopoietin-related protein 1
<400> 332
Met Lys Thr Phe Thr Trp Thr Leu Gly Val Leu Phe Phe Leu Leu Val
1 5 10 15
Asp Thr Gly His Cys Arg Gly
20
<210> 333
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein L1
<400> 333
Met Glu Gly Ala Ala Leu Leu Arg Val Ser Val Leu Cys Ile Trp Met
1 5 10 15
Ser Ala Leu Phe Leu Gly Val Gly Val Arg Ala
20 25
<210> 334
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Brain-specific serine protease 4
<400> 334
Met Val Val Ser Gly Ala Pro Pro Ala Leu Gly Gly Gly Cys Leu Gly
1 5 10 15
Thr Phe Thr Ser Leu Leu Leu Leu Ala Ser Thr Ala Ile Leu Asn Ala
20 25 30
<210> 335
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cocaine- and amphetamine-regulated transcript
protein
<400> 335
Met Glu Ser Ser Arg Val Arg Leu Leu Pro Leu Leu Gly Ala Ala Leu
1 5 10 15
Leu Leu Met Leu Pro Leu Leu Gly Thr Arg Ala
20 25
<210> 336
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 14
<400> 336
Met Lys Ile Ser Val Ala Ala Ile Pro Phe Phe Leu Leu Ile Thr Ile
1 5 10 15
Ala Leu Gly
<210> 337
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 4
<400> 337
Met Lys Leu Cys Val Thr Val Leu Ser Leu Leu Met Leu Val Ala Ala
1 5 10 15
Phe Cys Ser Pro Ala Leu Ser
20
<210> 338
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement component C6
<400> 338
Met Ala Arg Arg Ser Val Leu Tyr Phe Ile Leu Leu Asn Ala Leu Ile
1 5 10 15
Asn Lys Gly Gln Ala
20
<210> 339
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cytokine-like protein 1
<400> 339
Met Arg Thr Pro Gly Pro Leu Pro Val Leu Leu Leu Leu Leu Ala Gly
1 5 10 15
Ala Pro Ala Ala Arg Pro
20
<210> 340
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dickkopf-like protein 1
<400> 340
Met Gly Glu Ala Ser Pro Pro Ala Pro Ala Arg Arg His Leu Leu Val
1 5 10 15
Leu Leu Leu Leu Leu Ser Thr Leu Val Ile Pro Ser Ala Ala
20 25 30
<210> 341
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Deoxyribonuclease gamma
<400> 341
Met Ser Arg Glu Leu Ala Pro Leu Leu Leu Leu Leu Leu Ser Ile His
1 5 10 15
Ser Ala Leu Ala
20
<210> 342
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dentin sialophosphoprotein
<400> 342
Met Lys Ile Ile Thr Tyr Phe Cys Ile Trp Ala Val Ala Trp Ala
1 5 10 15
<210> 343
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EGF-like domain-containing protein 7
<400> 343
Met Arg Gly Ser Gln Glu Val Leu Leu Met Trp Leu Leu Val Leu Ala
1 5 10 15
Val Gly Gly Thr Glu His Ala
20
<210> 344
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endonuclease domain-containing 1 protein
<400> 344
Met Gly Thr Ala Arg Trp Leu Ala Leu Gly Ser Leu Phe Ala Leu Ala
1 5 10 15
Gly Leu Leu Glu Gly
20
<210> 345
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ectonucleotide pyrophosphatase/phosphodiesterase
family member 2
<400> 345
Met Ala Arg Arg Ser Ser Phe Gln Ser Cys Gln Ile Ile Ser Leu Phe
1 5 10 15
Thr Phe Ala Val Gly Val Asn Ile Cys Leu Gly
20 25
<210> 346
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Abhydrolase domain-containing protein FAM108B1
<400> 346
Met Asn Asn Leu Ser Phe Ser Glu Leu Cys Cys Leu Phe Cys Cys Pro
1 5 10 15
Pro Cys Pro Gly
20
<210> 347
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EGF-containing fibulin-like extracellular matrix
protein 2
<400> 347
Met Leu Pro Cys Ala Ser Cys Leu Pro Gly Ser Leu Leu Leu Trp Ala
1 5 10 15
Leu Leu Leu Leu Leu Leu Gly Ser Ala
20 25
<210> 348
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement factor H-related protein 5
<400> 348
Met Leu Leu Leu Phe Ser Val Ile Leu Ile Ser Trp Val Ser Thr Val
1 5 10 15
Gly Gly
<210> 349
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glypican-4
<400> 349
Met Ala Arg Phe Gly Leu Pro Ala Leu Leu Cys Thr Leu Ala Val Leu
1 5 10 15
Ser Ala
<210> 350
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig heavy chain V-I region V35
<400> 350
Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly
1 5 10 15
Ala His Ser
<210> 351
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interferon alpha-7
<400> 351
Met Ala Arg Ser Phe Ser Leu Leu Met Val Val Leu Val Leu Ser Tyr
1 5 10 15
Lys Ser Ile Cys Ser Leu Gly
20
<210> 352
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasma kallikrein-like protein 4
<400> 352
Met Val Ser Ala Ala Gly Leu Ser Gly Asp Gly Lys Met Arg Gly Val
1 5 10 15
Leu Leu Val Leu Leu Gly Leu Leu Tyr Ser Ser Thr Ser Cys
20 25 30
<210> 353
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Laminin subunit beta-2
<400> 353
Met Glu Leu Thr Ser Arg Glu Arg Gly Arg Gly Gln Pro Leu Pro Trp
1 5 10 15
Glu Leu Arg Leu Gly Leu Leu Leu Ser Val Leu Ala Ala Thr Leu Ala
20 25 30
<210> 354
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Laminin subunit gamma-3
<400> 354
Met Ala Ala Ala Ala Leu Leu Leu Gly Leu Ala Leu Leu Ala Pro Arg
1 5 10 15
Ala Ala Gly
<210> 355
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lipase member I
<400> 355
Met Arg Val Tyr Ile Phe Leu Cys Leu Met Cys Trp Val Arg Ser
1 5 10 15
<210> 356
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lactadherin
<400> 356
Met Pro Arg Pro Arg Leu Leu Ala Ala Leu Cys Gly Ala Leu Leu Cys
1 5 10 15
Ala Pro Ser Leu Leu Val Ala
20
<210> 357
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mannose receptor-like protein 1
<400> 357
Met Leu His Pro Glu Thr Ser Pro Gly Arg Gly His Leu Leu Ala Val
1 5 10 15
Leu Leu Ala Leu Leu Gly Thr Thr Trp Ala
20 25
<210> 358
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mucin-4
<400> 358
Met Lys Gly Ala Arg Trp Arg Arg Val Pro Trp Val Ser Leu Ser Cys
1 5 10 15
Leu Cys Leu Cys Leu Leu Pro His Val Val Pro Gly
20 25
<210> 359
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide NKG2D ligand 2
<400> 359
Met Ala Ala Ala Ala Ala Thr Lys Ile Leu Leu Cys Leu Pro Leu Leu
1 5 10 15
Leu Leu Leu Ser Gly Trp Ser Arg Ala
20 25
<210> 360
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Noelin-3
<400> 360
Met Ser Pro Pro Leu Leu Lys Leu Gly Ala Val Leu Ser Thr Met Ala
1 5 10 15
Met Ile Ser Asn Trp Met Ser
20
<210> 361
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Epididymal secretory protein E1
<400> 361
Met Arg Phe Leu Ala Ala Thr Phe Leu Leu Leu Ala Leu Ser Thr Ala
1 5 10 15
Ala Gln Ala
<210> 362
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Otogelin
<400> 362
Met Gly Val Leu Ala Ser Ala Leu Cys Trp Leu Leu Cys Val Trp Leu
1 5 10 15
Pro Trp Gly Glu Gln Ala
20
<210> 363
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide PATE-like protein B
<400> 363
Met Arg Lys Met Asn Thr Leu Leu Leu Val Ser Leu Ser Phe Leu Tyr
1 5 10 15
Leu Lys Glu
<210> 364
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Perforin-1
<400> 364
Met Ala Ala Arg Leu Leu Leu Leu Gly Ile Leu Leu Leu Leu Leu Pro
1 5 10 15
Leu Pro Val Pro Ala
20
<210> 365
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peptidoglycan recognition protein I-alpha
<400> 365
Met Gly Thr Leu Pro Trp Leu Leu Ala Phe Phe Ile Leu Gly Leu Gln
1 5 10 15
Ala
<210> 366
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prostatic acid phosphatase
<400> 366
Met Arg Ala Ala Pro Leu Leu Leu Ala Arg Ala Ala Ser Leu Ser Leu
1 5 10 15
Gly Phe Leu Phe Leu Leu Phe Phe Trp Leu Asp Arg Ser Val Leu Ala
20 25 30
<210> 367
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Basic salivary proline-rich protein 4
<400> 367
Met Leu Leu Ile Leu Leu Ser Val Ala Leu Leu Ala Leu Ser Ser Ala
1 5 10 15
<210> 368
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolactin receptor
<400> 368
Met Lys Glu Asn Val Ala Ser Ala Thr Val Phe Thr Leu Leu Leu Phe
1 5 10 15
Leu Asn Thr Cys Leu Leu Asn Gly
20
<210> 369
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serum amyloid A-4 protein
<400> 369
Met Arg Leu Phe Thr Gly Ile Val Phe Cys Ser Leu Val Met Gly Val
1 5 10 15
Thr Ser
<210> 370
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stromal cell-derived factor 2
<400> 370
Met Ala Val Val Pro Leu Leu Leu Leu Gly Gly Leu Trp Ser Ala Val
1 5 10 15
Gly Ala
<210> 371
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serpin E3
<400> 371
Met Pro Pro Phe Leu Ile Thr Leu Phe Leu Phe His Ser Cys Cys Leu
1 5 10 15
Arg Ala Asn Gly
20
<210> 372
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tenascin-N
<400> 372
Met Ser Leu Gln Glu Met Phe Arg Phe Pro Met Gly Leu Leu Leu Gly
1 5 10 15
Ser Val Leu Leu Val Ala Ser Ala Pro Ala Thr Leu
20 25
<210> 373
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Thioredoxin domain-containing protein 16
<400> 373
Met Phe Ser Gly Phe Asn Val Phe Arg Val Gly Ile Ser Phe Val Ile
1 5 10 15
Met Cys Ile Phe Tyr Met Pro Thr Val Asn Ser
20 25
<210> 374
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vascular endothelial growth factor receptor 2
<400> 374
Met Gln Ser Lys Val Leu Leu Ala Val Ala Leu Trp Leu Cys Val Glu
1 5 10 15
Thr Arg Ala
<210> 375
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide von Willebrand factor A domain-containing protein
5B1
<400> 375
Met Pro Gly Leu Leu Asn Trp Ile Thr Gly Ala Ala Leu Pro Leu Thr
1 5 10 15
Ala Ser
<210> 376
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP four-disulfide core domain protein 1
<400> 376
Met Pro Leu Thr Gly Val Gly Pro Gly Ser Cys Arg Arg Gln Ile Ile
1 5 10 15
Arg Ala Leu Cys Leu Leu Leu Leu Leu Leu His Ala Gly Ser Ala
20 25 30
<210> 377
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein
UNQ5830/PRO19650/PRO19816
<400> 377
Met Ala Ile Leu Met Leu Ser Leu Gln Leu Ile Leu Leu Leu Ile Pro
1 5 10 15
Ser Ile Ser
<210> 378
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein ENSP00000380674
<400> 378
Met Arg Pro Leu Leu Cys Ala Leu Ala Gly Leu Ala Leu Leu Cys Ala
1 5 10 15
Val Gly Ala Leu Ala
20
<210> 379
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein LOC116349
<400> 379
Met Pro Ala Val Phe Met Leu Ala Ser Ser Ser Ala Leu Gln Cys Gly
1 5 10 15
Arg Gly
<210> 380
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Anterior gradient protein 2 homolog
<400> 380
Met Glu Lys Ile Pro Val Ser Ala Phe Leu Leu Leu Val Ala Leu Ser
1 5 10 15
Tyr Thr Leu Ala
20
<210> 381
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like growth factor-binding protein complex
acid labile chain
<400> 381
Met Ala Leu Arg Lys Gly Gly Leu Ala Leu Ala Leu Leu Leu Leu Ser
1 5 10 15
Trp Val Ala Leu Gly Pro Arg Ser Leu Glu Gly
20 25
<210> 382
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein C-III
<400> 382
Met Gln Pro Arg Val Leu Leu Val Val Ala Leu Leu Ala Leu Leu Ala
1 5 10 15
Ser Ala Arg Ala
20
<210> 383
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 16
<400> 383
Met Lys Pro Arg Ala Arg Gly Trp Arg Gly Leu Ala Ala Leu Trp Met
1 5 10 15
Leu Leu Ala Gln Val Ala Glu Gln
20
<210> 384
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 8
<400> 384
Met Leu Pro Ala Pro Ala Ala Pro Arg Trp Pro Pro Leu Leu Leu Leu
1 5 10 15
Leu Leu Leu Leu Leu Pro Leu Ala Arg Gly Ala
20 25
<210> 385
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bone morphogenetic protein 1
<400> 385
Met Pro Gly Val Ala Arg Leu Pro Leu Leu Leu Gly Leu Leu Leu Leu
1 5 10 15
Pro Arg Pro Gly Arg Pro
20
<210> 386
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bactericidal/permeability-increasing protein-like
1
<400> 386
Met Ala Trp Ala Ser Arg Leu Gly Leu Leu Leu Ala Leu Leu Leu Pro
1 5 10 15
Val Val Gly Ala
20
<210> 387
<211> 40
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Scavenger receptor cysteine-rich type 1 protein
M160
<400> 387
Met Met Leu Pro Gln Asn Ser Trp His Ile Asp Phe Gly Arg Cys Cys
1 5 10 15
Cys His Gln Asn Leu Phe Ser Ala Val Val Thr Cys Ile Leu Leu Leu
20 25 30
Asn Ser Cys Phe Leu Ile Ser Ser
35 40
<210> 388
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cathepsin D
<400> 388
Met Gln Pro Ser Ser Leu Leu Pro Leu Ala Leu Cys Leu Leu Ala Ala
1 5 10 15
Pro Ala
<210> 389
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative cat eye syndrome critical region protein
9
<400> 389
Met Gln Ser His Leu Ala Pro Leu Ala Cys Ala Ala Ala Ala Gly Arg
1 5 10 15
Ala Gly Gly Ser Cys Gln Ala
20
<210> 390
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide UPF0670 protein C8orf55
<400> 390
Met Leu Gly Leu Leu Val Ala Leu Leu Ala Leu Gly Leu Ala Val Phe
1 5 10 15
Ala
<210> 391
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 3-like 1
<400> 391
Met Gln Val Ser Thr Ala Ala Leu Ala Val Leu Leu Cys Thr Met Ala
1 5 10 15
Leu Cys Asn Gln Val Leu Ser
20
<210> 392
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-2(VIII) chain
<400> 392
Met Leu Gly Thr Leu Thr Pro Leu Ser Ser Leu Leu Leu Leu Leu Leu
1 5 10 15
Val Leu Val Leu Gly Cys Gly Pro Arg Ala Ser Ser
20 25
<210> 393
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C18orf20
<400> 393
Met Ile Asn Leu His Arg Leu Cys Ile Ile His Val Val Ala Thr Leu
1 5 10 15
Leu Ser Thr Leu Leu Ser Leu Ile Ser Val Ala Ile Ser
20 25
<210> 394
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-X-C motif chemokine 14
<400> 394
Met Ser Leu Leu Pro Arg Arg Ala Pro Pro Val Ser Met Arg Leu Leu
1 5 10 15
Ala Ala Ala Leu Leu Leu
20
<210> 395
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 106
<400> 395
Met Arg Thr Phe Leu Phe Leu Phe Ala Val Leu Phe Phe Leu Thr Pro
1 5 10 15
Ala Lys Asn Ala
20
<210> 396
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Coagulation factor X
<400> 396
Met Gly Arg Pro Leu His Leu Val Leu Leu Ser Ala Ser Leu Ala Gly
1 5 10 15
Leu Leu Leu Leu Gly Glu Ser Leu Phe Ile Arg Arg Glu Gln Ala
20 25 30
<210> 397
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide INT-2 proto-oncogene protein
<400> 397
Met Gly Leu Ile Trp Leu Leu Leu Leu Ser Leu Leu Glu Pro Gly Trp
1 5 10 15
Pro
<210> 398
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leucine-rich repeat transmembrane protein FLRT2
<400> 398
Met Gly Leu Gln Thr Thr Lys Trp Pro Ser His Gly Ala Phe Phe Leu
1 5 10 15
Lys Ser Trp Leu Ile Ile Ser Leu Gly Leu Tyr Ser Gln Val Ser Lys
20 25 30
Leu Leu Ala
35
<210> 399
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-18-binding protein
<400> 399
Met Thr Met Arg His Asn Trp Thr Pro Asp Leu Ser Pro Leu Trp Val
1 5 10 15
Leu Leu Leu Cys Ala His Val Val Thr Leu Leu Val
20 25
<210> 400
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like growth factor-binding protein 7
<400> 400
Met Glu Arg Pro Ser Leu Arg Ala Leu Leu Leu Gly Ala Ala Gly Leu
1 5 10 15
Leu Leu Leu Leu Leu Pro Leu Ser Ser Ser
20 25
<210> 401
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serine protease inhibitor Kazal-type 7
<400> 401
Met Lys Ile Thr Gly Gly Leu Leu Leu Leu Cys Thr Val Val Tyr Phe
1 5 10 15
Cys Ser Ser
<210> 402
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Isthmin
<400> 402
Met Val Arg Leu Ala Ala Glu Leu Leu Leu Leu Leu Gly Leu Leu Leu
1 5 10 15
Leu Thr Leu His Ile Thr Val Leu Arg Gly Ser Gly Ala
20 25
<210> 403
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Laminin subunit beta-4
<400> 403
Met Gln Phe Gln Leu Thr Leu Phe Leu His Leu Gly Trp Leu Ser Tyr
1 5 10 15
Ser Lys Ala
<210> 404
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endothelial lipase
<400> 404
Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys
1 5 10 15
Phe Ala Ala Gly
20
<210> 405
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretoglobin-like protein LOC284402
<400> 405
Met Arg Val Thr Ser Ala Thr Cys Ala Leu Leu Leu Ala Leu Ile Cys
1 5 10 15
Ser Val Gln Leu Gly Asp Ala
20
<210> 406
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lymphocyte antigen 86
<400> 406
Met Lys Gly Phe Thr Ala Thr Leu Phe Leu Trp Thr Leu Ile Phe Pro
1 5 10 15
Ser Cys Ser Gly
20
<210> 407
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Netrin-1-like protein
<400> 407
Met Pro Val Thr Phe Ala Leu Leu Leu Leu Leu Gly Gln Ala Thr Ala
1 5 10 15
<210> 408
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide NHL repeat-containing protein 3
<400> 408
Met Ala Arg Phe Trp Val Cys Val Ala Gly Ala Gly Phe Phe Leu Ala
1 5 10 15
Phe Leu Val Leu His Ser Arg Phe Cys
20 25
<210> 409
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protocadherin-15
<400> 409
Met Phe Arg Gln Phe Tyr Leu Trp Thr Cys Leu Ala Ser Gly Ile Ile
1 5 10 15
Leu Gly Ser Leu Phe Glu Ile Cys Leu Gly
20 25
<210> 410
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pregnancy-specific beta-1-glycoprotein 5
<400> 410
Met Gly Pro Leu Ser Ala Pro Pro Cys Thr Gln His Ile Thr Trp Lys
1 5 10 15
Gly Leu Leu Leu Thr Ala Ser Leu Leu Asn Phe Trp Asn Leu Pro Ile
20 25 30
Thr Ala
<210> 411
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative peptide YY-2
<400> 411
Met Ala Thr Val Leu Leu Ala Leu Leu Val Tyr Leu Gly Ala Leu Val
1 5 10 15
Asp Ala
<210> 412
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ribonuclease K6
<400> 412
Met Val Leu Cys Phe Pro Leu Leu Leu Leu Leu Leu Val Leu Trp Gly
1 5 10 15
Pro Val Cys Pro Leu His Ala
20
<210> 413
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ribonuclease-like protein 12
<400> 413
Met Ile Ile Met Val Ile Ile Phe Leu Val Leu Leu Phe Trp Glu Asn
1 5 10 15
Glu Val Asn Asp
20
<210> 414
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Signal peptide, CUB and EGF-like domain-containing
protein 2
<400> 414
Met Gly Val Ala Gly Arg Asn Arg Pro Gly Ala Ala Trp Ala Val Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Pro Pro Leu Leu Leu Leu Ala Gly Ala
20 25 30
<210> 415
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serpin A9
<400> 415
Met Ala Ser Tyr Leu Tyr Gly Val Leu Phe Ala Val Gly Leu Cys Ala
1 5 10 15
Pro Ile Tyr Cys Val Ser Pro
20
<210> 416
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kunitz-type protease inhibitor 4
<400> 416
Met Lys Ser Ala Lys Leu Gly Phe Leu Leu Arg Phe Phe Ile Phe Cys
1 5 10 15
Ser Leu Asn Thr Leu Leu Leu Gly
20
<210> 417
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stanniocalcin-2
<400> 417
Met Cys Ala Glu Arg Leu Gly Gln Phe Met Thr Leu Ala Leu Val Leu
1 5 10 15
Ala Thr Phe Asp Pro Ala Arg Gly
20
<210> 418
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Thrombospondin type-1 domain-containing protein 4
<400> 418
Met Val Ser His Phe Met Gly Ser Leu Ser Val Leu Cys Phe Leu Leu
1 5 10 15
Leu Leu Gly Phe Gln Phe Val Cys Pro
20 25
<210> 419
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uromodulin
<400> 419
Met Gly Gln Pro Ser Leu Thr Trp Met Leu Met Val Val Val Ala Ser
1 5 10 15
Trp Phe Ile Thr Thr Ala Ala Thr
20
<210> 420
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-4
<400> 420
Met Ser Pro Arg Ser Cys Leu Arg Ser Leu Arg Leu Leu Val Phe Ala
1 5 10 15
Val Phe Ser Ala Ala Ala
20
<210> 421
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-7b
<400> 421
Met His Arg Asn Phe Arg Lys Trp Ile Phe Tyr Val Phe Leu Cys Phe
1 5 10 15
Gly Val Leu Tyr Val Lys Leu Gly Ala Leu Ser Ser Val Val Ala
20 25 30
<210> 422
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peptidase S1 domain-containing protein LOC136242
<400> 422
Met Lys Tyr Val Phe Tyr Leu Gly Val Leu Ala Gly Thr Phe Phe Phe
1 5 10 15
Ala Asp Ser
<210> 423
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q subcomponent subunit C
<400> 423
Met Asp Val Gly Pro Ser Ser Leu Pro His Leu Gly Leu Lys Leu Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Pro Leu Arg Gly Gln Ala
20 25
<210> 424
<211> 38
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C2orf66
<400> 424
Met Ile Ile Asp Ser Ser Arg Ile Pro Ser Phe Thr Gln Leu His Ser
1 5 10 15
Thr Met Thr Arg Ala Pro Leu Leu Leu Leu Cys Val Ala Leu Val Leu
20 25 30
Leu Gly His Val Asn Gly
35
<210> 425
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cerebellin-4
<400> 425
Met Gly Ser Gly Arg Arg Ala Leu Ser Ala Val Pro Ala Val Leu Leu
1 5 10 15
Val Leu Thr Leu Pro Gly Leu Pro Val Trp Ala
20 25
<210> 426
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Coiled-coil domain-containing protein 3
<400> 426
Met Leu Arg Gln Leu Leu Leu Ala Ala Leu Cys Leu Ala Gly Pro Pro
1 5 10 15
Ala Pro Ala Arg Ala
20
<210> 427
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Eotaxin
<400> 427
Met Lys Val Ser Ala Ala Leu Leu Trp Leu Leu Leu Ile Ala Ala Ala
1 5 10 15
Phe Ser Pro Gln Gly Leu Ala
20
<210> 428
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 23
<400> 428
Met Lys Val Ser Val Ala Ala Leu Ser Cys Leu Met Leu Val Thr Ala
1 5 10 15
Leu Gly Ser Gln Ala
20
<210> 429
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 3
<400> 429
Met Gln Val Ser Thr Ala Ala Leu Ala Val Leu Leu Cys Thr Met Ala
1 5 10 15
Leu Cys Asn Gln Phe Ser Ala
20
<210> 430
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Colipase-like protein C6orf127
<400> 430
Met Met Leu Pro Gln Trp Leu Leu Leu Leu Phe Leu Leu Phe Phe Phe
1 5 10 15
Leu Phe Leu Leu Thr Arg Gly
20
<210> 431
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Retinoic acid early transcript 1G protein
<400> 431
Met Ala Ala Ala Ala Ser Pro Ala Phe Leu Leu Arg Leu Pro Leu Leu
1 5 10 15
Leu Leu Leu Ser Ser Trp Cys Arg Thr
20 25
<210> 432
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-type lectin domain family 11 member A
<400> 432
Met Gln Ala Ala Trp Leu Leu Gly Ala Leu Val Val Pro Gln Leu Leu
1 5 10 15
Gly Phe Gly His Gly
20
<210> 433
<211> 38
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-4(IV) chain
<400> 433
Met Trp Ser Leu His Ile Val Leu Met Arg Cys Ser Phe Arg Leu Thr
1 5 10 15
Lys Ser Leu Ala Thr Gly Pro Trp Ser Leu Ile Leu Ile Leu Phe Ser
20 25 30
Val Gln Tyr Val Tyr Gly
35
<210> 434
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C3 and PZP-like alpha-2-macroglobulin
domain-containing protein 8
<400> 434
Met Ser Gly Ala Leu Leu Trp Pro Leu Leu Pro Leu Leu Leu Leu Leu
1 5 10 15
Leu Ser Ala Arg Asp Gly Val Arg Ala
20 25
<210> 435
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cysteine-rich secretory protein 2
<400> 435
Met Ala Leu Leu Pro Val Leu Phe Leu Val Thr Val Leu Leu Pro Ser
1 5 10 15
Leu Pro Ala Glu Gly
20
<210> 436
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chymotrypsinogen B
<400> 436
Met Ala Ser Leu Trp Leu Leu Ser Cys Phe Ser Leu Val Gly Ala Ala
1 5 10 15
Phe Gly
<210> 437
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cystatin-SA
<400> 437
Met Ala Trp Pro Leu Cys Thr Leu Leu Leu Leu Leu Ala Thr Gln Ala
1 5 10 15
Val Ala Leu Ala
20
<210> 438
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Coagulation factor VII
<400> 438
Met Val Ser Gln Ala Leu Arg Leu Leu Cys Leu Leu Leu Gly Leu Gln
1 5 10 15
Gly Cys Leu Ala
20
<210> 439
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibroblast growth factor-binding protein 1
<400> 439
Met Lys Ile Cys Ser Leu Thr Leu Leu Ser Phe Leu Leu Leu Ala Ala
1 5 10 15
Gln Val Leu Leu Val Glu Gly
20
<210> 440
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Growth/differentiation factor 5
<400> 440
Met Arg Leu Pro Lys Leu Leu Thr Phe Leu Leu Trp Tyr Leu Ala Trp
1 5 10 15
Leu Asp Leu Glu Phe Ile Cys Thr Val Leu Gly
20 25
<210> 441
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glypican-2
<400> 441
Met Ser Ala Leu Arg Pro Leu Leu Leu Leu Leu Leu Pro Leu Cys Pro
1 5 10 15
Gly Pro Gly Pro Gly Pro Gly
20
<210> 442
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hemicentin-1
<400> 442
Met Ile Ser Trp Glu Val Val His Thr Val Phe Leu Phe Ala Leu Leu
1 5 10 15
Tyr Ser Ser Leu Ala
20
<210> 443
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hyaluronan and proteoglycan link protein 4
<400> 443
Met Val Cys Ala Arg Ala Ala Leu Gly Pro Gly Ala Leu Trp Ala Ala
1 5 10 15
Ala Trp Gly Val Leu Leu Leu Thr Ala Pro Ala Gly Ala
20 25
<210> 444
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interferon beta
<400> 444
Met Thr Asn Lys Cys Leu Leu Gln Ile Ala Leu Leu Leu Cys Phe Ser
1 5 10 15
Thr Thr Ala Leu Ser
20
<210> 445
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastric intrinsic factor
<400> 445
Met Ala Trp Phe Ala Leu Tyr Leu Leu Ser Leu Leu Trp Ala Thr Ala
1 5 10 15
Gly Thr
<210> 446
<211> 37
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Immunoglobulin lambda-like polypeptide 1
<400> 446
Met Arg Pro Gly Thr Gly Gln Gly Gly Leu Glu Ala Pro Gly Glu Pro
1 5 10 15
Gly Pro Asn Leu Arg Gln Arg Trp Pro Leu Leu Leu Leu Gly Leu Ala
20 25 30
Val Val Thr His Gly
35
<210> 447
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-21
<400> 447
Met Glu Arg Ile Val Ile Cys Leu Met Val Ile Phe Leu Gly Thr Leu
1 5 10 15
Val His Lys Ser Ser Ser
20
<210> 448
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-29
<400> 448
Met Ala Ala Ala Trp Thr Val Val Leu Val Thr Leu Val Leu Gly Leu
1 5 10 15
Ala Val Ala
<210> 449
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-4 receptor alpha chain
<400> 449
Met Gly Trp Leu Cys Ser Gly Leu Leu Phe Pro Val Ser Cys Leu Val
1 5 10 15
Leu Leu Gln Val Ala Ser Ser Gly Asn
20 25
<210> 450
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kin of IRRE-like protein 3
<400> 450
Met Lys Pro Phe Gln Leu Asp Leu Leu Phe Val Cys Phe Phe Leu Phe
1 5 10 15
Ser Gln Glu Leu Gly
20
<210> 451
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-13
<400> 451
Met Trp Pro Leu Ala Leu Val Ile Ala Ser Leu Thr Leu Ala Leu Ser
1 5 10 15
<210> 452
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-9
<400> 452
Met Lys Leu Gly Leu Leu Cys Ala Leu Leu Ser Leu Leu Ala Gly
1 5 10 15
<210> 453
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leukemia inhibitory factor
<400> 453
Met Lys Val Leu Ala Ala Gly Val Val Pro Leu Leu Leu Val Leu His
1 5 10 15
Trp Lys His Gly Ala Gly
20
<210> 454
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Long palate, lung and nasal epithelium
carcinoma-associated protein 4
<400> 454
Met Trp Met Ala Trp Cys Val Ala Ala Leu Ser Val Val Ala Val Cys
1 5 10 15
Gly Thr
<210> 455
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stromelysin-3
<400> 455
Met Ala Pro Ala Ala Trp Leu Arg Ser Ala Ala Ala Arg Ala Leu Leu
1 5 10 15
Pro Pro Met Leu Leu Leu Leu Leu Gln Pro Pro Pro Leu Leu Ala
20 25 30
<210> 456
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrix metalloproteinase-19
<400> 456
Met Asn Cys Gln Gln Leu Trp Leu Gly Phe Leu Leu Pro Met Thr Val
1 5 10 15
Ser Gly
<210> 457
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endomucin
<400> 457
Met Glu Leu Leu Gln Val Thr Ile Leu Phe Leu Leu Pro Ser Ile Cys
1 5 10 15
Ser Ser
<210> 458
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Nucleobindin-2
<400> 458
Met Arg Trp Arg Thr Ile Leu Leu Gln Tyr Cys Phe Leu Leu Ile Thr
1 5 10 15
Cys Leu Leu Thr Ala Leu Glu Ala
20
<210> 459
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Polymeric immunoglobulin receptor
<400> 459
Met Leu Leu Phe Val Leu Thr Cys Leu Leu Ala Val Phe Pro Ala Ile
1 5 10 15
Ser Thr
<210> 460
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serine protease 27
<400> 460
Met Arg Arg Pro Ala Ala Val Pro Leu Leu Leu Leu Leu Cys Phe Gly
1 5 10 15
Ser Gln Arg Ala Lys Ala
20
<210> 461
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Stromal cell-derived factor 1
<400> 461
Met Asn Ala Lys Val Val Val Val Leu Val Leu Val Leu Thr Ala Leu
1 5 10 15
Cys Leu Ser Asp Gly
20
<210> 462
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Signal-regulatory protein delta
<400> 462
Met Pro Ile Pro Ala Ser Pro Leu His Pro Pro Leu Pro Ser Leu Leu
1 5 10 15
Leu Tyr Leu Leu Leu Glu Leu Ala Gly Val Thr His Val
20 25
<210> 463
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Slit homolog 1 protein
<400> 463
Met Ala Leu Thr Pro Gly Trp Gly Ser Ser Ala Gly Pro Val Arg Pro
1 5 10 15
Glu Leu Trp Leu Leu Leu Trp Ala Ala Ala Trp Arg Leu Gly Ala Ser
20 25 30
Ala
<210> 464
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sortilin-related receptor
<400> 464
Met Ala Thr Arg Ser Ser Arg Arg Glu Ser Arg Leu Pro Phe Leu Phe
1 5 10 15
Thr Leu Val Ala Leu Leu Pro Pro Gly Ala Leu Cys
20 25
<210> 465
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Short palate, lung and nasal epithelium
carcinoma-associated protein 3
<400> 465
Met Met Cys Pro Leu Trp Arg Leu Leu Ile Phe Leu Gly Leu Leu Ala
1 5 10 15
Leu Pro Leu Ala Pro
20
<210> 466
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tryptase beta-2
<400> 466
Met Leu Asn Leu Leu Leu Leu Ala Leu Pro Val Leu Ala Ser Arg Ala
1 5 10 15
Tyr Ala
<210> 467
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-11
<400> 467
Met Arg Ala Arg Pro Gln Val Cys Glu Ala Leu Leu Phe Ala Leu Ala
1 5 10 15
Leu Gln Thr Gly Val Cys Tyr Gly
20
<210> 468
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fractalkine
<400> 468
Met Ala Pro Ile Ser Leu Ser Trp Leu Leu Arg Leu Ala Thr Phe Cys
1 5 10 15
His Leu Thr Val Leu Leu Ala Gly
20
<210> 469
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig-like domain-containing protein ENSP00000270642
<400> 469
Met Pro Pro Pro Ala Pro Gly Ala Arg Leu Arg Leu Leu Ala Ala Ala
1 5 10 15
Ala Leu Ala Gly Leu Ala Val Ile Ser Arg Gly Leu Leu Ser
20 25 30
<210> 470
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein M
<400> 470
Met Phe His Gln Ile Trp Ala Ala Leu Leu Tyr Phe Tyr Gly Ile Ile
1 5 10 15
Leu Asn Ser Ile Tyr Gln
20
<210> 471
<211> 39
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Artemin
<400> 471
Met Glu Leu Gly Leu Gly Gly Leu Ser Thr Leu Ser His Cys Pro Trp
1 5 10 15
Pro Arg Gln Gln Pro Ala Leu Trp Pro Thr Leu Ala Ala Leu Ala Leu
20 25 30
Leu Ser Ser Val Ala Glu Ala
35
<210> 472
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acid sphingomyelinase-like phosphodiesterase 3a
<400> 472
Met Ala Leu Val Arg Ala Leu Val Cys Cys Leu Leu Thr Ala Trp His
1 5 10 15
Cys Arg Ser Gly Leu Gly
20
<210> 473
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q tumor necrosis factor-related
protein 1
<400> 473
Met Gly Ser Arg Gly Gln Gly Leu Leu Leu Ala Tyr Cys Leu Leu Leu
1 5 10 15
Ala Phe Ala Ser Gly Leu Val Leu Ser
20 25
<210> 474
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 28
<400> 474
Met Gln Gln Arg Gly Leu Ala Ile Val Ala Leu Ala Val Cys Ala Ala
1 5 10 15
Leu His Ala
<210> 475
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide T-cell surface glycoprotein CD8 alpha chain
<400> 475
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu
1 5 10 15
His Ala Ala Arg Pro
20
<210> 476
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cat eye syndrome critical region protein 1
<400> 476
Met Leu Val Asp Gly Pro Ser Glu Arg Pro Ala Leu Cys Phe Leu Leu
1 5 10 15
Leu Ala Val Ala Met Ser Phe Phe Gly Ser Ala Leu Ser
20 25
<210> 477
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-1(II) chain
<400> 477
Met Ile Arg Leu Gly Ala Pro Gln Thr Leu Val Leu Leu Thr Leu Leu
1 5 10 15
Val Ala Ala Val Leu Arg Cys Gln Gly
20 25
<210> 478
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-3(IX) chain
<400> 478
Met Ala Gly Pro Arg Ala Cys Ala Pro Leu Leu Leu Leu Leu Leu Leu
1 5 10 15
Gly Glu Leu Leu Ala Ala Ala Gly Ala
20 25
<210> 479
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-1(XX) chain
<400> 479
Met Ser Ser Gly Asp Pro Ala His Leu Gly Leu Cys Leu Trp Leu Trp
1 5 10 15
Leu Gly Ala Thr Leu Gly
20
<210> 480
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peptidase inhibitor R3HDML
<400> 480
Met Pro Leu Leu Pro Ser Thr Val Gly Leu Ala Gly Leu Leu Phe Trp
1 5 10 15
Ala Gly Gln Ala Val Asn Ala Leu
20
<210> 481
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Crumbs homolog 1
<400> 481
Met Ala Leu Lys Asn Ile Asn Tyr Leu Leu Ile Phe Tyr Leu Ser Phe
1 5 10 15
Ser Leu Leu Ile Tyr Ile Lys Asn Ser
20 25
<210> 482
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 116
<400> 482
Met Ser Val Met Lys Pro Cys Leu Met Thr Ile Ala Ile Leu Met Ile
1 5 10 15
Leu Ala Gln Lys Thr Pro Gly
20
<210> 483
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 125
<400> 483
Met Asn Ile Leu Met Leu Thr Phe Ile Ile Cys Gly Leu Leu Thr Arg
1 5 10 15
Val Thr Lys Gly
20
<210> 484
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein FAM55D
<400> 484
Met Lys Ile Ser Met Ile Asn Tyr Lys Ser Leu Leu Ala Leu Leu Phe
1 5 10 15
Ile Leu Ala Ser Trp Ile Ile Phe Thr Val Phe
20 25
<210> 485
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibronectin type III domain-containing protein 7
<400> 485
Met Ala Gly Gly Arg Glu Thr Cys Leu Pro Leu Ile Gly Phe Ile Leu
1 5 10 15
Ile Cys Leu Lys Met Val Ala Ser Ala Lys
20 25
<210> 486
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin growth factor-like family member 4
<400> 486
Met Val Pro Arg Ile Ser Ala Ala Ile Phe Ile Phe Glu Leu Leu Gly
1 5 10 15
Ser Asn Ser
<210> 487
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Immunoglobulin superfamily member 21
<400> 487
Met Arg Thr Ala Pro Ser Leu Arg Arg Cys Val Cys Leu Leu Leu Ala
1 5 10 15
Ala Ile Leu Asp Leu Ala Arg Gly
20
<210> 488
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-17A
<400> 488
Met Thr Pro Gly Lys Thr Ser Leu Val Ser Leu Leu Leu Leu Leu Ser
1 5 10 15
Leu Glu Ala Ile Val Lys Ala
20
<210> 489
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Inhibin beta B chain
<400> 489
Met Asp Gly Leu Pro Gly Arg Ala Leu Gly Ala Ala Cys Leu Leu Leu
1 5 10 15
Leu Ala Ala Gly Trp Leu Gly Pro Glu Ala Trp Gly
20 25
<210> 490
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Intelectin-2
<400> 490
Met Leu Ser Met Leu Arg Thr Met Thr Arg Leu Cys Phe Leu Leu Phe
1 5 10 15
Phe Ser Val Ala Thr Ser Gly Cys Ser Ala
20 25
<210> 491
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leucine-rich repeat and calponin homology
domain-containing protein 3
<400> 491
Met Ala Ala Ala Gly Leu Val Ala Val Ala Ala Ala Ala Glu Tyr Ser
1 5 10 15
Gly Thr Val Ala Ser Gly
20
<210> 492
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurotrypsin
<400> 492
Met Thr Leu Ala Arg Phe Val Leu Ala Leu Met Leu Gly Ala Leu Pro
1 5 10 15
Glu Val Val Gly
20
<210> 493
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurturin
<400> 493
Met Gln Arg Trp Lys Ala Ala Ala Leu Ala Ser Val Leu Cys Ser Ser
1 5 10 15
Val Leu Ser
<210> 494
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protease-associated domain-containing protein of
21 kDa
<400> 494
Met Val Pro Gly Ala Ala Gly Trp Cys Cys Leu Val Leu Trp Leu Pro
1 5 10 15
Ala Cys Val Ala Ala
20
<210> 495
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proprotein convertase subtilisin/kexin type 9
<400> 495
Met Gly Thr Val Ser Ser Arg Arg Ser Trp Trp Pro Leu Pro Leu Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Leu Gly Pro Ala Gly Ala Arg Ala
20 25 30
<210> 496
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pigment epithelium-derived factor
<400> 496
Met Gln Ala Leu Val Leu Leu Leu Cys Ile Gly Ala Leu Leu Gly His
1 5 10 15
Ser Ser Cys
<210> 497
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peptidase inhibitor 15
<400> 497
Met Ile Ala Ile Ser Ala Val Ser Ser Ala Leu Leu Phe Ser Leu Leu
1 5 10 15
Cys Glu Ala
<210> 498
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasminogen-related protein A
<400> 498
Met Glu His Lys Glu Val Val Leu Leu Leu Leu Leu Phe Leu Lys Ser
1 5 10 15
Gly Gln Gly
<210> 499
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Podocan
<400> 499
Met Ala Gln Ser Arg Val Leu Leu Leu Leu Leu Leu Leu Pro Pro Gln
1 5 10 15
Leu His Leu
<210> 500
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative testis-specific prion protein
<400> 500
Met Gln His Ser Leu Val Phe Phe Phe Ala Val Ile Leu His Leu Ser
1 5 10 15
His Leu
<210> 501
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Salivary acidic proline-rich phosphoprotein 1/2
<400> 501
Met Leu Leu Ile Leu Leu Ser Val Ala Leu Leu Ala Phe Ser Ser Ala
1 5 10 15
<210> 502
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide PC3-secreted microprotein
<400> 502
Met Ala Leu Arg Met Leu Trp Ala Gly Gln Ala Lys Gly Ile Leu Gly
1 5 10 15
Gly Trp Gly Ile Ile Cys Leu Val Met Ser Leu Leu Leu Gln His Pro
20 25 30
Gly Val Tyr
35
<210> 503
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretogranin-2
<400> 503
Met Ala Glu Ala Lys Thr His Trp Leu Gly Ala Ala Leu Ser Leu Ile
1 5 10 15
Pro Leu Ile Phe Leu Ile Ser Gly Ala Glu Ala
20 25
<210> 504
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secreted and transmembrane protein 1
<400> 504
Met Gln Thr Cys Pro Leu Ala Phe Pro Gly His Val Ser Gln Ala Leu
1 5 10 15
Gly Thr Leu Leu Phe Leu Ala Ala Ser Leu Ser Ala
20 25
<210> 505
<211> 36
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Semaphorin-3D
<400> 505
Met Asn Ala Asn Lys Asp Glu Arg Leu Lys Ala Arg Ser Gln Asp Phe
1 5 10 15
His Leu Phe Pro Ala Leu Met Met Leu Ser Met Thr Met Leu Phe Leu
20 25 30
Pro Val Thr Gly
35
<210> 506
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretoglobin family 3A member 2
<400> 506
Met Lys Leu Val Thr Ile Phe Leu Leu Val Thr Ile Ser Leu Cys Ser
1 5 10 15
Tyr Ser Ala Thr Ala
20
<210> 507
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sialate O-acetylesterase
<400> 507
Met Val Ala Pro Gly Leu Val Leu Gly Leu Val Leu Pro Leu Ile Leu
1 5 10 15
Trp Ala Asp Arg Ser Ala Gly
20
<210> 508
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sialic acid-binding Ig-like lectin 6
<400> 508
Met Gln Gly Ala Gln Glu Ala Ser Ala Ser Glu Met Leu Pro Leu Leu
1 5 10 15
Leu Pro Leu Leu Trp Ala Gly Ala Leu Ala
20 25
<210> 509
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide SPARC
<400> 509
Met Arg Ala Trp Ile Phe Phe Leu Leu Cys Leu Ala Gly Arg Ala Leu
1 5 10 15
Ala
<210> 510
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hepatocellular carcinoma-associated protein TD26
<400> 510
Met Pro Val Pro Ala Leu Cys Leu Leu Trp Ala Leu Ala Met Val Thr
1 5 10 15
Arg Pro Ala Ser Ala
20
<210> 511
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trypsin-X3
<400> 511
Met Lys Phe Ile Leu Leu Trp Ala Leu Leu Asn Leu Thr Val Ala Leu
1 5 10 15
Ala
<210> 512
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Urocortin-2
<400> 512
Met Thr Arg Cys Ala Leu Leu Leu Leu Met Val Leu Met Leu Gly Arg
1 5 10 15
Val Leu Val
<210> 513
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proto-oncogene protein Wnt-3
<400> 513
Met Glu Pro His Leu Leu Gly Leu Leu Leu Gly Leu Leu Leu Gly Gly
1 5 10 15
Thr Arg Val Leu Ala
20
<210> 514
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein FLJ37543
<400> 514
Met Leu Ala Pro Leu Phe Leu Cys Cys Leu Arg Asn Leu Phe Arg Lys
1 5 10 15
Leu Ile Ser
<210> 515
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein ENSP00000381830
<400> 515
Met Ser Phe Glu Tyr Arg His Tyr Lys Arg Glu Ala Lys Ile Cys Thr
1 5 10 15
Cys Arg Gly Gly Trp Ala His Val Leu Leu Cys Ile Gly Val Ser Gln
20 25 30
Gly Ala Cys
35
<210> 516
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-2-antiplasmin
<400> 516
Met Ala Leu Leu Trp Gly Leu Leu Val Leu Ser Trp Ser Cys Leu Gln
1 5 10 15
Gly Pro Cys Ser Val Phe Ser Pro Val Ser Ala
20 25
<210> 517
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Angiotensin-converting enzyme
<400> 517
Met Gly Ala Ala Ser Gly Arg Arg Gly Pro Gly Leu Leu Leu Pro Leu
1 5 10 15
Pro Leu Leu Leu Leu Leu Pro Pro Gln Pro Ala Leu Ala
20 25
<210> 518
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Angiopoietin-1
<400> 518
Met Thr Val Phe Leu Ser Phe Ala Phe Leu Ala Ala Ile Leu Thr
1 5 10 15
<210> 519
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein C-IV
<400> 519
Met Ser Leu Leu Arg Asn Arg Leu Gln Ala Leu Pro Ala Leu Cys Leu
1 5 10 15
Cys Val Leu Val Leu Ala Cys Ile Gly Ala
20 25
<210> 520
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Brain-derived neurotrophic factor
<400> 520
Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met
1 5 10 15
Lys Ala
<210> 521
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C1orf54
<400> 521
Met Asp Val Leu Phe Val Ala Ile Phe Ala Val Pro Leu Ile Leu Gly
1 5 10 15
<210> 522
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cerebellin-3
<400> 522
Met Leu Gly Ala Lys Pro His Trp Leu Pro Gly Pro Leu His Ser Pro
1 5 10 15
Gly Leu Pro Leu Val Leu Val Leu Leu Ala Leu Gly Ala Gly Trp Ala
20 25 30
<210> 523
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxypeptidase B2
<400> 523
Met Lys Leu Cys Ser Leu Ala Val Leu Val Pro Ile Val Leu Phe Cys
1 5 10 15
Glu Gln His Val Phe Ala
20
<210> 524
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 22
<400> 524
Met Asp Arg Leu Gln Thr Ala Leu Leu Val Val Leu Val Leu Leu Ala
1 5 10 15
Val Ala Leu Gln Ala Thr Glu Ala
20
<210> 525
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Colipase-like protein C6orf126
<400> 525
Met Ala Ala Ala Leu Ala Leu Val Ala Gly Val Leu Ser Gly Ala Val
1 5 10 15
Leu Pro Leu Trp Ser
20
<210> 526
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C7orf34
<400> 526
Met Pro Pro Leu Ala Pro Gln Leu Cys Arg Ala Val Phe Leu Val Pro
1 5 10 15
Ile Leu Leu Leu Leu Gln Val Lys Pro Leu Asn Gly
20 25
<210> 527
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein canopy homolog 4
<400> 527
Met Gly Pro Val Arg Leu Gly Ile Leu Leu Phe Leu Phe Leu Ala Val
1 5 10 15
His Glu Ala Trp Ala
20
<210> 528
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-2(VI) chain
<400> 528
Met Leu Gln Gly Thr Cys Ser Val Leu Leu Leu Trp Gly Ile Leu Gly
1 5 10 15
Ala Ile Gln Ala
20
<210> 529
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tenascin-X
<400> 529
Met Met Pro Ala Gln Tyr Ala Leu Thr Ser Ser Leu Val Leu Leu Val
1 5 10 15
Leu Leu Ser Thr Ala Arg Ala
20
<210> 530
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endothelin-3
<400> 530
Met Glu Pro Gly Leu Trp Leu Leu Phe Gly Leu Thr Val Thr Ser Ala
1 5 10 15
<210> 531
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Endothelial cell-specific molecule 1
<400> 531
Met Lys Ser Val Leu Leu Leu Thr Thr Leu Leu Val Pro Ala His Leu
1 5 10 15
Val Ala Ala
<210> 532
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein eyes shut homolog
<400> 532
Met Thr Asp Lys Ser Ile Val Ile Leu Ser Leu Met Val Phe His Ser
1 5 10 15
Ser Phe Ile Asn Gly
20
<210> 533
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Coagulation factor XI
<400> 533
Met Ile Phe Leu Tyr Gln Val Val His Phe Ile Leu Phe Thr Ser Val
1 5 10 15
Ser Gly
<210> 534
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Immunoglobulin alpha Fc receptor
<400> 534
Met Asp Pro Lys Gln Thr Thr Leu Leu Cys Leu Val Leu Cys Leu Gly
1 5 10 15
Gln Arg Ile Gln Ala
20
<210> 535
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Low affinity immunoglobulin gamma Fc region
receptor III-B
<400> 535
Met Trp Gln Leu Leu Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala
1 5 10 15
<210> 536
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Granzyme A
<400> 536
Met Arg Asn Ser Tyr Arg Phe Leu Ala Ser Ser Leu Ser Val Val Val
1 5 10 15
Ser Leu Leu Leu Ile Pro Glu Asp Val Cys
20 25
<210> 537
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hyaluronan and proteoglycan link protein 3
<400> 537
Met Gly Leu Leu Leu Leu Val Pro Leu Leu Leu Leu Pro Gly Ser Tyr
1 5 10 15
Gly
<210> 538
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-28B
<400> 538
Met Thr Gly Asp Cys Met Pro Val Leu Val Leu Met Ala Ala Val Leu
1 5 10 15
Thr Val Thr Gly Ala Val Pro Val Ala
20 25
<210> 539
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pancreatic secretory trypsin inhibitor
<400> 539
Met Lys Val Thr Gly Ile Phe Leu Leu Ser Ala Leu Ala Leu Leu Ser
1 5 10 15
Leu Ser Gly Asn Thr Gly Ala
20
<210> 540
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallistatin
<400> 540
Met His Leu Ile Asp Tyr Leu Leu Leu Leu Leu Val Gly Leu Leu Ala
1 5 10 15
Leu Ser His Gly
20
<210> 541
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-12
<400> 541
Met Gly Leu Ser Ile Phe Leu Leu Leu Cys Val Leu Gly Leu Ser Gln
1 5 10 15
Ala
<210> 542
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Keratinocyte differentiation-associated protein
<400> 542
Met Lys Ile Pro Val Leu Pro Ala Val Val Leu Leu Ser Leu Leu Val
1 5 10 15
Leu His Ser Ala Gln Gly
20
<210> 543
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative lipocalin 1-like protein 1
<400> 543
Met Lys Pro Leu Leu Leu Ala Ile Ser Leu Ser Leu Ile Ala Ala Leu
1 5 10 15
Gln Ala
<210> 544
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lysozyme C
<400> 544
Met Lys Ala Leu Ile Val Leu Gly Leu Val Leu Leu Ser Val Thr Val
1 5 10 15
Gln Gly
<210> 545
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide MAM domain-containing protein 2
<400> 545
Met Leu Leu Arg Gly Val Leu Leu Ala Leu Gln Ala Leu Gln Leu Ala
1 5 10 15
Gly Ala
<210> 546
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrilysin
<400> 546
Met Arg Leu Thr Val Leu Cys Ala Val Cys Leu Leu Pro Gly Ser Leu
1 5 10 15
Ala
<210> 547
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Oxytocin-neurophysin 1
<400> 547
Met Ala Gly Pro Ser Leu Ala Cys Cys Leu Leu Gly Leu Leu Ala Leu
1 5 10 15
Thr Ser Ala
<210> 548
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide NPIP-like protein LOC440350
<400> 548
Met Arg Leu Arg Phe Trp Leu Leu Ile Trp Leu Leu Leu Gly Phe Ile
1 5 10 15
Ser His
<210> 549
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Phospholipase A2, membrane associated
<400> 549
Met Lys Thr Leu Leu Leu Leu Ala Val Ile Met Ile Phe Gly Leu Leu
1 5 10 15
Gln Ala His Gly
20
<210> 550
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Group XIIB secretory phospholipase A2-like protein
<400> 550
Met Lys Leu Ala Ser Gly Phe Leu Val Leu Trp Leu Ser Leu Gly Gly
1 5 10 15
Gly Leu Ala
<210> 551
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Basement membrane-specific heparan sulfate
proteoglycan core protein
<400> 551
Met Gly Trp Arg Ala Ala Gly Ala Leu Leu Leu Ala Leu Leu Leu His
1 5 10 15
Gly Arg Leu Leu Ala
20
<210> 552
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasma glutamate carboxypeptidase
<400> 552
Met Lys Phe Leu Ile Phe Ala Phe Phe Gly Gly Val His Leu Leu Ser
1 5 10 15
Leu Cys Ser Gly
20
<210> 553
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Melanocyte protein Pmel 17
<400> 553
Met Asp Leu Val Leu Lys Arg Cys Leu Leu His Leu Ala Val Ile Gly
1 5 10 15
Ala Leu Leu Ala Val Gly Ala Thr
20
<210> 554
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vitamin K-dependent protein Z
<400> 554
Met Ala Gly Cys Val Pro Leu Leu Gln Gly Leu Val Leu Val Leu Ala
1 5 10 15
Leu His Arg Val Glu Pro Ser
20
<210> 555
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sulfhydryl oxidase 1
<400> 555
Met Arg Arg Cys Asn Ser Gly Ser Gly Pro Pro Pro Ser Leu Leu Leu
1 5 10 15
Leu Leu Leu Trp Leu Leu Ala Val Pro Gly Ala Asn Ala
20 25
<210> 556
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Regenerating islet-derived protein 3 gamma
<400> 556
Met Leu Pro Pro Met Ala Leu Pro Ser Val Ser Trp Met Leu Leu Ser
1 5 10 15
Cys Leu Ile Leu Leu Cys Gln Val Gln Gly
20 25
<210> 557
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretoglobin family 1C member 1
<400> 557
Met Lys Gly Ser Arg Ala Leu Leu Leu Val Ala Leu Thr Leu Phe Cys
1 5 10 15
Ile Cys Arg Met Ala Thr Gly
20
<210> 558
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sex hormone-binding globulin
<400> 558
Met Glu Ser Arg Gly Pro Leu Ala Thr Ser Arg Leu Leu Leu Leu Leu
1 5 10 15
Leu Leu Leu Leu Leu Arg His Thr Arg Gln Gly Trp Ala
20 25
<210> 559
<211> 40
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tomoregulin-2
<400> 559
Met Val Leu Trp Glu Ser Pro Arg Gln Cys Ser Ser Trp Thr Leu Cys
1 5 10 15
Glu Gly Phe Cys Trp Leu Leu Leu Leu Pro Val Met Leu Leu Ile Val
20 25 30
Ala Arg Pro Val Lys Leu Ala Ala
35 40
<210> 560
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trefoil factor 2
<400> 560
Met Gly Arg Arg Asp Ala Gln Leu Leu Ala Ala Leu Leu Val Leu Gly
1 5 10 15
Leu Cys Ala Leu Ala Gly Ser
20
<210> 561
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tachykinin-4
<400> 561
Met Leu Pro Cys Leu Ala Leu Leu Leu Leu Met Glu Leu Ser Val Cys
1 5 10 15
Thr Val Ala
<210> 562
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tumor necrosis factor receptor superfamily member
1A
<400> 562
Met Gly Leu Ser Thr Val Pro Asp Leu Leu Leu Pro Leu Val Leu Leu
1 5 10 15
Glu Leu Leu Val Gly
20
<210> 563
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tumor necrosis factor receptor superfamily member
5
<400> 563
Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr
1 5 10 15
Ala Val His Pro
20
<210> 564
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Usherin
<400> 564
Met Asn Cys Pro Val Leu Ser Leu Gly Ser Gly Phe Leu Phe Gln Val
1 5 10 15
Ile Glu Met Leu Ile Phe Ala Tyr Phe Ala Ser Ile Ser Leu Thr
20 25 30
<210> 565
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Urotensin-2
<400> 565
Met Tyr Lys Leu Ala Ser Cys Cys Leu Leu Phe Ile Gly Phe Leu Asn
1 5 10 15
Pro Leu Leu Ser
20
<210> 566
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide V-set and transmembrane domain-containing protein
2A
<400> 566
Met Met Gly Ile Phe Leu Val Tyr Val Gly Phe Val Phe Phe Ser Val
1 5 10 15
Leu Tyr Val Gln Gln Gly Leu
20
<210> 567
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-6
<400> 567
Met Leu Pro Pro Leu Pro Ser Arg Leu Gly Leu Leu Leu Leu Leu Leu
1 5 10 15
Leu Cys Pro Ala His Val Gly Gly
20
<210> 568
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein FP248
<400> 568
Met Gly Thr Gly Gly Ser Leu Leu Cys Gly Cys Ser Leu Val Leu Ser
1 5 10 15
Cys Leu Cys Pro Ser Ala Ser
20
<210> 569
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative serine protease LOC138652
<400> 569
Met Gly Tyr Cys Gln Gly Val Ser Gln Val Ala Val Val Leu Leu Met
1 5 10 15
Phe Pro Lys Glu Lys Glu Ala
20
<210> 570
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Zona pellucida sperm-binding protein 3
<400> 570
Met Glu Leu Ser Tyr Arg Leu Phe Ile Cys Leu Leu Leu Trp Gly Ser
1 5 10 15
Thr Glu Leu Cys Tyr Pro
20
<210> 571
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Signal peptide, CUB and EGF-like domain-containing
protein 3
<400> 571
Met Gly Ser Gly Arg Val Pro Gly Leu Cys Leu Leu Val Leu Leu Val
1 5 10 15
His Ala Arg Ala
20
<210> 572
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-type natriuretic peptide
<400> 572
Met His Leu Ser Gln Leu Leu Ala Cys Ala Leu Leu Leu Thr Leu Leu
1 5 10 15
Ser Leu Arg Pro Ser Glu Ala
20
<210> 573
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Antithrombin-III
<400> 573
Met Tyr Ser Asn Val Ile Gly Thr Val Thr Ser Gly Lys Arg Lys Val
1 5 10 15
Tyr Leu Leu Ser Leu Leu Leu Ile Gly Phe Trp Asp Cys Val Thr Cys
20 25 30
<210> 574
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Acid sphingomyelinase-like phosphodiesterase 3b
<400> 574
Met Arg Leu Leu Ala Trp Leu Ile Phe Leu Ala Asn Trp Gly Gly Ala
1 5 10 15
Arg Ala
<210> 575
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 13
<400> 575
Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala
1 5 10 15
Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly
20 25
<210> 576
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide B melanoma antigen 1
<400> 576
Met Ala Ala Arg Ala Val Phe Leu Ala Leu Ser Ala Gln Leu Leu Gln
1 5 10 15
Ala
<210> 577
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q subcomponent subunit A
<400> 577
Met Glu Gly Pro Arg Gly Trp Leu Val Leu Cys Val Leu Ala Ile Ser
1 5 10 15
Leu Ala Ser Met Val Thr
20
<210> 578
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q tumor necrosis factor-related
protein 2
<400> 578
Met Ile Pro Trp Val Leu Leu Ala Cys Ala Leu Pro Cys Ala Ala
1 5 10 15
<210> 579
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C1orf187
<400> 579
Met Ala Gly Pro Ala Ile His Thr Ala Pro Met Leu Phe Leu Val Leu
1 5 10 15
Leu Leu Pro Leu Glu Leu Ser Leu Ala
20 25
<210> 580
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carbonic anhydrase-related protein 11
<400> 580
Met Gly Ala Ala Ala Arg Leu Ser Ala Pro Arg Ala Leu Val Leu Trp
1 5 10 15
Ala Ala Leu Gly Ala Ala Ala
20
<210> 581
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 19
<400> 581
Met Ala Leu Leu Leu Ala Leu Ser Leu Leu Val Leu Trp Thr Ser Pro
1 5 10 15
Ala Pro Thr Leu Ser
20
<210> 582
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Monocyte differentiation antigen CD14
<400> 582
Met Glu Arg Ala Ser Cys Leu Leu Leu Leu Leu Leu Pro Leu Val His
1 5 10 15
Val Ser Ala
<210> 583
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide CUB domain-containing protein 1
<400> 583
Met Ala Gly Leu Asn Cys Gly Val Ser Ile Ala Leu Leu Gly Val Leu
1 5 10 15
Leu Leu Gly Ala Ala Arg Leu Pro Arg Gly Ala Glu Ala
20 25
<210> 584
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide UPF0669 protein C6orf120
<400> 584
Met Ala Ala Pro Arg Gly Arg Ala Ala Pro Trp Thr Thr Ala Leu Leu
1 5 10 15
Leu Leu Leu Ala Ser Gln Val Leu Ser Pro Gly Ser Cys Ala
20 25 30
<210> 585
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Chitinase-3-like protein 1
<400> 585
Met Gly Val Lys Ala Ser Gln Thr Gly Phe Val Val Leu Val Leu Leu
1 5 10 15
Gln Cys Cys Ser Ala
20
<210> 586
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C18orf54
<400> 586
Met Ala Lys Ser Lys Thr Lys His Arg Leu Cys Ser Gln Glu Ser Ser
1 5 10 15
Val Ser Ala Leu Leu Ala Ser Cys Thr Leu Ser Gly Ser Asn Ser
20 25 30
<210> 587
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cystatin-8
<400> 587
Met Pro Arg Cys Arg Trp Leu Ser Leu Ile Leu Leu Thr Ile Pro Leu
1 5 10 15
Ala Leu Val Ala Arg
20
<210> 588
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secreted frizzled-related protein 3
<400> 588
Met Val Cys Gly Ser Pro Gly Gly Met Leu Leu Leu Arg Ala Gly Leu
1 5 10 15
Leu Ala Leu Ala Ala Leu Cys Leu Leu Arg Val Pro Gly Ala Arg Ala
20 25 30
<210> 589
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 118
<400> 589
Met Lys Leu Leu Leu Leu Ala Leu Pro Met Leu Val Leu Leu Pro Gln
1 5 10 15
Val Ile Pro
<210> 590
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 133
<400> 590
Met Lys Ile His Val Phe Leu Phe Val Leu Phe Phe Phe Leu Val Pro
1 5 10 15
Ile Ala Thr Arg Val Lys Cys
20
<210> 591
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 1
<400> 591
Met Arg Thr Ser Tyr Leu Leu Leu Phe Thr Leu Cys Leu Leu Leu Ser
1 5 10 15
Glu Met Ala Ser Gly
20
<210> 592
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Dermokine
<400> 592
Met Lys Phe Gln Gly Pro Leu Ala Cys Leu Leu Leu Ala Leu Cys Leu
1 5 10 15
Gly Ser Gly Glu Ala
20
<210> 593
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein FAM24A
<400> 593
Met Ala Lys Met Phe Asp Leu Arg Thr Lys Ile Met Ile Gly Ile Gly
1 5 10 15
Ser Ser Leu Leu Val Ala Ala Met Val Leu Leu Ser Val Val Phe Cys
20 25 30
<210> 594
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibrinogen-like protein 1
<400> 594
Met Ala Lys Val Phe Ser Phe Ile Leu Val Thr Thr Ala Leu Thr Met
1 5 10 15
Gly Arg Glu Ile Ser Ala
20
<210> 595
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Folate receptor gamma
<400> 595
Met Ala Trp Gln Met Met Gln Leu Leu Leu Leu Ala Leu Val Thr Ala
1 5 10 15
Ala Gly Ser Ala Gln Pro Arg
20
<210> 596
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Growth/differentiation factor 11
<400> 596
Met Val Leu Ala Ala Pro Leu Leu Leu Gly Phe Leu Leu Leu Ala Leu
1 5 10 15
Glu Leu Arg Pro Arg Gly Glu Ala
20
<210> 597
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Granzyme K
<400> 597
Met Thr Lys Phe Ser Ser Phe Ser Leu Phe Phe Leu Ile Val Gly Ala
1 5 10 15
Tyr Met Thr His Val Cys Phe Asn
20
<210> 598
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Major histocompatibility complex class I-related
gene protein
<400> 598
Met Gly Glu Leu Met Ala Phe Leu Leu Pro Leu Ile Ile Val Leu Met
1 5 10 15
Val Lys His Ser Asp Ser
20
<210> 599
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Immunoglobulin superfamily member 1
<400> 599
Met Thr Leu Asp Arg Pro Gly Glu Gly Ala Thr Met Leu Lys Thr Phe
1 5 10 15
Thr
<210> 600
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-1 receptor antagonist protein
<400> 600
Met Glu Ile Cys Arg Gly Leu Arg Ser His Leu Ile Thr Leu Leu Leu
1 5 10 15
Phe Leu Phe His Ser Glu Thr Ile Cys
20 25
<210> 601
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serine protease inhibitor Kazal-type 5
<400> 601
Met Lys Ile Ala Thr Val Ser Val Leu Leu Pro Leu Ala Leu Cys Leu
1 5 10 15
Ile Gln Asp Ala Ala Ser
20
<210> 602
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Immunoglobulin superfamily containing leucine-rich
repeat protein
<400> 602
Met Gln Glu Leu His Leu Leu Trp Trp Ala Leu Leu Leu Gly Leu Ala
1 5 10 15
Gln Ala
<210> 603
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative killer cell immunoglobulin-like receptor
like protein KIR3DP1
<400> 603
Met Ser Leu Met Val Ile Ser Met Ala Cys Val Gly Phe Phe Leu Leu
1 5 10 15
Gln Gly Ala Trp Thr
20
<210> 604
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Laminin subunit alpha-4
<400> 604
Met Ala Leu Ser Ser Ala Trp Arg Ser Val Leu Pro Leu Trp Leu Leu
1 5 10 15
Trp Ser Ala Ala Cys Ser Arg Ala
20
<210> 605
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leucine zipper protein 2
<400> 605
Met Lys Phe Ser Pro Ala His Tyr Leu Leu Pro Leu Leu Pro Ala Leu
1 5 10 15
Val Leu Ser
<210> 606
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Sushi, nidogen and EGF-like domain-containing
protein 1
<400> 606
Met Arg His Gly Val Ala Trp Ala Leu Leu Val Ala Ala Ala Leu Gly
1 5 10 15
Leu Gly Ala Arg Gly Val Arg Gly
20
<210> 607
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein-lysine 6-oxidase
<400> 607
Met Arg Phe Ala Trp Thr Val Leu Leu Leu Gly Pro Leu Gln Leu Cys
1 5 10 15
Ala Leu Val His Cys
20
<210> 608
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuroblastoma suppressor of tumorigenicity 1
<400> 608
Met Met Leu Arg Val Leu Val Gly Ala Val Leu Pro Ala Met Leu
1 5 10 15
<210> 609
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurotensin/neuromedin N
<400> 609
Met Met Ala Gly Met Lys Ile Gln Leu Val Cys Met Leu Leu Leu Ala
1 5 10 15
Phe Ser Ser Trp Ser Leu Cys
20
<210> 610
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Group 3 secretory phospholipase A2
<400> 610
Met Gly Val Gln Ala Gly Leu Phe Gly Met Leu Gly Phe Leu Gly Val
1 5 10 15
Ala Leu Gly
<210> 611
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Placenta growth factor
<400> 611
Met Pro Val Met Arg Leu Phe Pro Cys Phe Leu Gln Leu Leu Ala Gly
1 5 10 15
Leu Ala
<210> 612
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Pregnancy-specific beta-1-glycoprotein 11
<400> 612
Met Gly Pro Leu Ser Ala Pro Pro Cys Thr Glu His Ile Lys Trp Lys
1 5 10 15
Gly Leu Leu Leu Thr Ala Leu Leu Leu Asn Phe Trp Asn Leu Pro Thr
20 25 30
Thr Ala
<210> 613
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ribonuclease T2
<400> 613
Met Arg Pro Ala Ala Leu Arg Gly Ala Leu Leu Gly Cys Leu Cys Leu
1 5 10 15
Ala Leu Leu Cys Leu Gly Gly Ala
20
<210> 614
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide R-spondin-1
<400> 614
Met Arg Leu Gly Leu Cys Val Val Ala Leu Val Leu Ser Trp Thr His
1 5 10 15
Leu Thr Ile Ser
20
<210> 615
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Semaphorin-3E
<400> 615
Met Ala Ser Ala Gly His Ile Ile Thr Leu Leu Leu Trp Gly Tyr Leu
1 5 10 15
Leu Glu Leu Trp Thr Gly Gly His Thr
20 25
<210> 616
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kunitz-type protease inhibitor 1
<400> 616
Met Ala Pro Ala Arg Thr Met Ala Arg Ala Arg Leu Ala Pro Ala Gly
1 5 10 15
Ile Pro Ala Val Ala Leu Trp Leu Leu Cys Thr Leu Gly Leu Gln Gly
20 25 30
Thr Gln Ala
35
<210> 617
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Transmembrane protein 25
<400> 617
Met Ala Leu Pro Pro Gly Pro Ala Ala Leu Arg His Thr Leu Leu Leu
1 5 10 15
Leu Pro Ala Leu Leu Ser Ser Gly Trp Gly
20 25
<210> 618
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP four-disulfide core domain protein 10A
<400> 618
Met Ala Pro Gln Thr Leu Leu Pro Val Leu Val Leu Cys Val Leu Leu
1 5 10 15
Leu Gln Ala Gln Gly
20
<210> 619
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP four-disulfide core domain protein 3
<400> 619
Met Met Leu Ser Cys Leu Phe Leu Leu Lys Ala Leu Leu Ala Leu Gly
1 5 10 15
Ser Leu Glu Ser Trp Ile Thr Ala
20
<210> 620
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-10a
<400> 620
Met Gly Ser Ala His Pro Arg Pro Trp Leu Arg Leu Arg Pro Gln Pro
1 5 10 15
Gln Pro Arg Pro Ala Leu Trp Val Leu Leu Phe Phe Leu Leu Leu Leu
20 25 30
Ala Ala Ala
35
<210> 621
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Abhydrolase domain-containing protein 9
<400> 621
Met Pro Glu Leu Val Val Thr Ala Leu Leu Ala Pro Ser Arg Leu Ser
1 5 10 15
Leu Lys Leu Leu Arg Ala Phe Met Trp Ser Leu Val Phe Ser Val Ala
20 25 30
Leu Val Ala
35
<210> 622
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAM 12
<400> 622
Met Ala Ala Arg Pro Leu Pro Val Ser Pro Ala Arg Ala Leu Leu Leu
1 5 10 15
Ala Leu Ala Gly Ala Leu Leu Ala Pro Cys Glu Ala
20 25
<210> 623
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 2
<400> 623
Met Asp Pro Pro Ala Gly Ala Ala Arg Arg Leu Leu Cys Pro Ala Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Leu Pro Pro Pro Leu Leu Pro
20 25
<210> 624
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bone morphogenetic protein 3b
<400> 624
Met Ala His Val Pro Ala Arg Thr Ser Pro Gly Pro Gly Pro Gln Leu
1 5 10 15
Leu Leu Leu Leu Leu Pro Leu Phe Leu Leu Leu Leu Arg Asp Val Ala
20 25 30
Gly
<210> 625
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q-like protein 4
<400> 625
Met Val Leu Leu Leu Leu Val Ala Ile Pro Leu Leu Val His Ser
1 5 10 15
<210> 626
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1r subcomponent-like protein
<400> 626
Met Pro Gly Pro Arg Val Trp Gly Lys Tyr Leu Trp Arg Ser Pro His
1 5 10 15
Ser Lys Gly Cys Pro Gly Ala Met Trp Trp Leu Leu Leu Trp Gly Val
20 25 30
Leu Gln Ala
35
<210> 627
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C1orf56
<400> 627
Met Val Pro Ala Ala Gly Ala Leu Leu Trp Val Leu Leu Leu Asn Leu
1 5 10 15
Gly Pro Arg Ala Ala Gly Ala
20
<210> 628
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cathelicidin antimicrobial peptide
<400> 628
Met Lys Thr Gln Arg Asp Gly His Ser Leu Gly Arg Trp Ser Leu Val
1 5 10 15
Leu Leu Leu Leu Gly Leu Val Met Pro Leu Ala Ile Ile Ala
20 25 30
<210> 629
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxypeptidase A2
<400> 629
Met Ala Met Arg Leu Ile Leu Phe Phe Gly Ala Leu Phe Gly His Ile
1 5 10 15
<210> 630
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 4-like
<400> 630
Met Lys Leu Cys Val Thr Val Leu Ser Leu Leu Val Leu Val Ala Ala
1 5 10 15
Phe Cys Ser Leu Ala Leu Ser
20
<210> 631
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 13
<400> 631
Met Lys Val Ser Ala Val Leu Leu Cys Leu Leu Leu Met Thr Ala Ala
1 5 10 15
<210> 632
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neural cell adhesion molecule L1-like protein
<400> 632
Met Glu Pro Leu Leu Leu Gly Arg Gly Leu Ile Val Tyr Leu Met Phe
1 5 10 15
Leu Leu Leu Lys Phe Ser Lys Ala
20
<210> 633
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C4-B
<400> 633
Met Arg Leu Leu Trp Gly Leu Ile Trp Ala Ser Ser Phe Phe Thr Leu
1 5 10 15
Ser Leu Gln
<210> 634
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-1(IX) chain
<400> 634
Met Lys Thr Cys Trp Lys Ile Pro Val Phe Phe Phe Val Cys Ser Phe
1 5 10 15
Leu Glu Pro Trp Ala Ser Ala
20
<210> 635
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cartilage oligomeric matrix protein
<400> 635
Met Val Pro Asp Thr Ala Cys Val Leu Leu Leu Thr Leu Ala Ala Leu
1 5 10 15
Gly Ala Ser Gly
20
<210> 636
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cysteine-rich secretory protein LCCL
domain-containing 1
<400> 636
Met Lys Cys Thr Ala Arg Glu Trp Leu Arg Val Thr Thr Val Leu Phe
1 5 10 15
Met Ala Arg Ala Ile Pro Ala
20
<210> 637
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide UPF0556 protein C19orf10
<400> 637
Met Ala Ala Pro Ser Gly Gly Trp Asn Gly Val Gly Ala Ser Leu Trp
1 5 10 15
Ala Ala Leu Leu Leu Gly Ala Val Ala Leu Arg Pro Ala Glu Ala
20 25 30
<210> 638
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C19orf36
<400> 638
Met Ala Leu Leu Leu Cys Leu Val Cys Leu Thr Ala Ala Leu Ala
1 5 10 15
<210> 639
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Granulocyte-macrophage colony-stimulating factor
receptor subunit alpha
<400> 639
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
1 5 10 15
Ala Phe Leu Leu Ile Pro
20
<210> 640
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 103
<400> 640
Met Arg Ile His Tyr Leu Leu Phe Ala Leu Leu Phe Leu Phe Leu Val
1 5 10 15
Pro Val Pro Gly His Gly
20
<210> 641
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 123
<400> 641
Met Lys Leu Leu Leu Leu Thr Leu Thr Val Leu Leu Leu Leu Ser Gln
1 5 10 15
Leu Thr Pro Gly
20
<210> 642
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 130
<400> 642
Met Lys Leu His Ser Leu Ile Ser Val Leu Leu Leu Phe Val Thr Leu
1 5 10 15
Ile Pro Lys Gly Lys Thr
20
<210> 643
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 2
<400> 643
Met Arg Val Leu Tyr Leu Leu Phe Ser Phe Leu Phe Ile Phe Leu Met
1 5 10 15
Pro Leu Pro Gly Val Phe Gly
20
<210> 644
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ectonucleoside triphosphate diphosphohydrolase 5
<400> 644
Met Ala Thr Ser Trp Gly Thr Val Phe Phe Met Leu Val Val Ser Cys
1 5 10 15
Val Cys Ser Ala
20
<210> 645
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein FAM55A
<400> 645
Met Ser Ser Asn Thr Met Leu Gln Lys Thr Leu Leu Ile Leu Ile Ser
1 5 10 15
Phe Ser Val Val Thr
20
<210> 646
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein FAM3B
<400> 646
Met Arg Pro Leu Ala Gly Gly Leu Leu Lys Val Val Phe Val Val Phe
1 5 10 15
Ala Ser Leu Cys Ala Trp Tyr Ser Gly Tyr Leu Leu Ala
20 25
<210> 647
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement factor H-related protein 4
<400> 647
Met Leu Leu Leu Ile Asn Val Ile Leu Thr Leu Trp Val Ser Cys Ala
1 5 10 15
Asn Gly Gln
<210> 648
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glucose-fructose oxidoreductase domain-containing
protein 1
<400> 648
Met Leu Pro Gly Val Gly Val Phe Gly Thr Ser Leu Thr Ala Arg Val
1 5 10 15
Ile Ile Pro Leu Leu
20
<210> 649
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hemopexin
<400> 649
Met Ala Arg Val Leu Gly Ala Pro Val Ala Leu Gly Leu Trp Ser Leu
1 5 10 15
Cys Trp Ser Leu Ala Ile Ala
20
<210> 650
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig heavy chain V-I region HG3
<400> 650
Met Asp Trp Thr Trp Arg Val Phe Cys Leu Leu Ala Val Ala Pro Gly
1 5 10 15
Ala His Ser
<210> 651
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like growth factor-binding protein 4
<400> 651
Met Leu Pro Leu Cys Leu Val Ala Ala Leu Leu Leu Ala Ala Gly Pro
1 5 10 15
Gly Pro Ser Leu Gly
20
<210> 652
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-17B
<400> 652
Met Asp Trp Pro His Asn Leu Leu Phe Leu Leu Thr Ile Ser Ile Phe
1 5 10 15
Leu Gly Leu Gly
20
<210> 653
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-1 receptor type I
<400> 653
Met Lys Val Leu Leu Arg Leu Ile Cys Phe Ile Ala Leu Leu Ile Ser
1 5 10 15
Ser
<210> 654
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-5
<400> 654
Met Arg Met Leu Leu His Leu Ser Leu Leu Ala Leu Gly Ala Ala Tyr
1 5 10 15
Val Tyr Ala
<210> 655
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Inhibin beta A chain
<400> 655
Met Pro Leu Leu Trp Leu Arg Gly Phe Leu Leu Ala Ser Cys Trp Ile
1 5 10 15
Ile Val Arg Ser
20
<210> 656
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Epididymal-specific lipocalin-12
<400> 656
Met Arg Leu Leu Cys Gly Leu Trp Leu Trp Leu Ser Leu Leu Lys Val
1 5 10 15
Leu Gln Ala
<210> 657
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ly-6/neurotoxin-like protein 1
<400> 657
Met Thr Pro Leu Leu Thr Leu Ile Leu Val Val Leu Met Gly Leu Pro
1 5 10 15
Leu Ala Gln Ala
20
<210> 658
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide 72 kDa type IV collagenase
<400> 658
Met Glu Ala Leu Met Ala Arg Gly Ala Leu Thr Gly Pro Leu Arg Ala
1 5 10 15
Leu Cys Leu Leu Gly Cys Leu Leu Ser His Ala Ala Ala
20 25
<210> 659
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Serine protease MPN2
<400> 659
Met Ala Ala Pro Ala Ser Val Met Gly Pro Leu Gly Pro Ser Ala Leu
1 5 10 15
Gly Leu Leu Leu Leu Leu Leu Val Val Ala Pro Pro Arg Val Ala Ala
20 25 30
<210> 660
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neuronal pentraxin-like protein C16orf38
<400> 660
Met Gly Cys Ser Trp Arg Lys Thr Leu Ser Phe Phe Leu Val Phe Val
1 5 10 15
Pro Ile Tyr Leu His Gly Ala Ser Ser
20 25
<210> 661
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Neurexophilin-1
<400> 661
Met Gln Ala Ala Cys Trp Tyr Val Leu Phe Leu Leu Gln Pro Thr Val
1 5 10 15
Tyr Leu Val Thr Cys
20
<210> 662
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Group IIE secretory phospholipase A2
<400> 662
Met Lys Ser Pro His Val Leu Val Phe Leu Cys Leu Leu Val Ala Leu
1 5 10 15
Val Thr Gly
<210> 663
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Platelet-derived growth factor D
<400> 663
Met His Arg Leu Ile Phe Val Tyr Thr Leu Ile Cys Ala Asn Phe Cys
1 5 10 15
Ser Cys
<210> 664
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Platelet factor 4
<400> 664
Met Ser Ser Ala Ala Gly Phe Cys Ala Ser Arg Pro Gly Leu Leu Phe
1 5 10 15
Leu Gly Leu Leu Leu Leu Pro Leu Val Val Ala Phe Ala Ser Ala
20 25 30
<210> 665
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Periostin
<400> 665
Met Ile Pro Phe Leu Pro Met Phe Ser Leu Leu Leu Leu Leu Ile Val
1 5 10 15
Asn Pro Ile Asn Ala
20
<210> 666
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Basic salivary proline-rich protein 3
<400> 666
Met Leu Leu Ile Leu Leu Ser Val Ala Leu Leu Ala Leu Ser Ser Ala
1 5 10 15
<210> 667
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prostasin
<400> 667
Met Ala Gln Lys Gly Val Leu Gly Pro Gly Gln Leu Gly Ala Val Ala
1 5 10 15
Ile Leu Leu Tyr Leu Gly Leu Leu Arg Ser Gly Thr Gly
20 25
<210> 668
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Poliovirus receptor
<400> 668
Met Ala Arg Ala Met Ala Ala Ala Trp Pro Leu Leu Leu Val Ala Leu
1 5 10 15
Leu Val Leu Ser
20
<210> 669
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Retinol-binding protein 3
<400> 669
Met Met Arg Glu Trp Val Leu Leu Met Ser Val Leu Leu Cys Gly Leu
1 5 10 15
Ala
<210> 670
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WAP, kazal, immunoglobulin, kunitz and NTR
domain-containing protein 1
<400> 670
Met Pro Ala Leu Arg Pro Leu Leu Pro Leu Leu Leu Leu Leu Arg Leu
1 5 10 15
Thr Ser Gly
<210> 671
<211> 35
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-5a
<400> 671
Met Lys Lys Ser Ile Gly Ile Leu Ser Pro Gly Val Ala Leu Gly Met
1 5 10 15
Ala Gly Ser Ala Met Ser Ser Lys Phe Phe Leu Val Ala Leu Ala Ile
20 25 30
Phe Phe Ser
35
<210> 672
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized lipocalin UNQ2541/PRO6093
<400> 672
Met Met Ser Phe Leu Leu Gly Ala Ile Leu Thr Leu Leu Trp Ala Pro
1 5 10 15
Thr Ala Gln Ala
20
<210> 673
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Zona pellucida sperm-binding protein 4
<400> 673
Met Trp Leu Leu Arg Cys Val Leu Leu Cys Val Ser Leu Ser Leu Ala
1 5 10 15
Val Ser
<210> 674
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Alpha-amylase 1
<400> 674
Met Lys Leu Phe Trp Leu Leu Phe Thr Ile Gly Phe Cys Trp Ala
1 5 10 15
<210> 675
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q-like protein 2
<400> 675
Met Ala Leu Gly Leu Leu Ile Ala Val Pro Leu Leu Leu Gln Ala Ala
1 5 10 15
Pro Arg Gly Ala Ala
20
<210> 676
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q tumor necrosis factor-related
protein 9
<400> 676
Met Arg Ile Trp Trp Leu Leu Leu Ala Ile Glu Ile Cys Thr Gly Asn
1 5 10 15
Ile Asn Ser
<210> 677
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide C-C motif chemokine 24
<400> 677
Met Ala Gly Leu Met Thr Ile Val Thr Ser Leu Leu Phe Leu Gly Val
1 5 10 15
Cys Ala His His Ile Ile Pro Thr Gly Ser
20 25
<210> 678
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C4orf26
<400> 678
Met Ala Arg Arg His Cys Phe Ser Tyr Trp Leu Leu Val Cys Trp Leu
1 5 10 15
Val Val Thr Val Ala Glu Gly
20
<210> 679
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide CD166 antigen
<400> 679
Met Glu Ser Lys Gly Ala Ser Ser Cys Arg Leu Leu Phe Cys Leu Leu
1 5 10 15
Ile Ser Ala Thr Val Phe Arg Pro Gly Leu Gly
20 25
<210> 680
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C14orf93
<400> 680
Met Ser Phe Ser Ala Thr Ile Leu Phe Ser Pro Pro Ser Gly Ser Glu
1 5 10 15
Ala
<210> 681
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-5(VI) chain
<400> 681
Met Lys Ile Leu Leu Ile Ile Phe Val Leu Ile Ile Trp Thr Glu Thr
1 5 10 15
Leu Ala
<210> 682
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement component C8 gamma chain
<400> 682
Met Leu Pro Pro Gly Thr Ala Thr Leu Leu Thr Leu Leu Leu Ala Ala
1 5 10 15
Gly Ser Leu Gly
20
<210> 683
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-1(X) chain
<400> 683
Met Leu Pro Gln Ile Pro Phe Leu Leu Leu Val Ser Leu Asn Leu Val
1 5 10 15
His Gly
<210> 684
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein C17orf69
<400> 684
Met His Pro Trp Leu Gly Ser Ala Leu Gly Phe Pro Lys Cys Arg Gly
1 5 10 15
<210> 685
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cysteine-rich motor neuron 2 protein
<400> 685
Met Ala Gly Val Gly Ala Ala Ala Leu Ser Leu Leu Leu His Leu Gly
1 5 10 15
Ala Leu Ala Leu Ala Ala Gly Ala Glu Gly
20 25
<210> 686
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cysteine-rich secretory protein 3
<400> 686
Met Thr Leu Phe Pro Val Leu Leu Phe Leu Val Ala Gly Leu Leu Pro
1 5 10 15
Ser Phe Pro Ala
20
<210> 687
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Macrophage colony-stimulating factor 1
<400> 687
Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu
1 5 10 15
Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr
20 25 30
<210> 688
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-defensin 108B
<400> 688
Met Arg Ile Ala Val Leu Leu Phe Ala Ile Phe Phe Phe Met Ser Gln
1 5 10 15
Val Leu Pro Ala Arg Gly
20
<210> 689
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement decay-accelerating factor
<400> 689
Met Thr Val Ala Arg Pro Ser Val Pro Ala Ala Leu Pro Leu Leu Gly
1 5 10 15
Glu Leu Pro Arg Leu Leu Leu Leu Val Leu Leu Cys Leu Pro Ala Val
20 25 30
Trp Gly
<210> 690
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Estradiol 17-beta-dehydrogenase 11
<400> 690
Met Lys Phe Leu Leu Asp Ile Leu Leu Leu Leu Pro Leu Leu Ile Val
1 5 10 15
Cys Ser Leu
<210> 691
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EMILIN-2
<400> 691
Met Trp Gln Pro Arg Arg Pro Trp Pro Arg Val Pro Trp Arg Trp Ala
1 5 10 15
Leu Ala Leu Leu Ala Leu Val Gly Ala Gly Leu Cys His Ala
20 25 30
<210> 692
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EGF-containing fibulin-like extracellular matrix
protein 1
<400> 692
Met Leu Lys Ala Leu Phe Leu Thr Met Leu Thr Leu Ala Leu Val Lys
1 5 10 15
Ser
<210> 693
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibroblast growth factor 5
<400> 693
Met Ser Leu Ser Phe Leu Leu Leu Leu Phe Phe Ser His Leu Ile Leu
1 5 10 15
Ser
<210> 694
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibroblast growth factor receptor 4
<400> 694
Met Arg Leu Leu Leu Ala Leu Leu Gly Val Leu Leu Ser Val Pro Gly
1 5 10 15
Pro Pro Val Leu Ser
20
<210> 695
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glia-derived nexin
<400> 695
Met Asn Trp His Leu Pro Leu Phe Leu Leu Ala Ser Val Thr Leu Pro
1 5 10 15
Ser Ile Cys
<210> 696
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gamma-glutamyl hydrolase
<400> 696
Met Ala Ser Pro Gly Cys Leu Leu Cys Val Leu Gly Leu Leu Leu Cys
1 5 10 15
Gly Ala Ala Ser Leu Glu Leu Ser
20
<210> 697
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glutathione peroxidase 3
<400> 697
Met Ala Arg Leu Leu Gln Ala Ser Cys Leu Leu Ser Leu Leu Leu Ala
1 5 10 15
Gly Phe Val Ser
20
<210> 698
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Histatin-1
<400> 698
Met Lys Phe Phe Val Phe Ala Leu Val Leu Ala Leu Met Ile Ser Met
1 5 10 15
Ile Ser Ala
<210> 699
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Insulin-like growth factor II
<400> 699
Met Gly Ile Pro Met Gly Lys Ser Met Leu Val Leu Leu Thr Phe Leu
1 5 10 15
Ala Phe Ala Ser Cys Cys Ile Ala
20
<210> 700
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-19
<400> 700
Met Lys Leu Gln Cys Val Ser Leu Trp Leu Leu Gly Thr Ile Leu Ile
1 5 10 15
Leu Cys Ser Val Asp Asn His Gly
20
<210> 701
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-32
<400> 701
Met Cys Phe Pro Lys Val Leu Ser Asp Asp Met Lys Lys Leu Lys Ala
1 5 10 15
Arg Met Val Met Leu Leu Pro Thr Ser Ala Gln Gly Leu Gly
20 25 30
<210> 702
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Inter-alpha-trypsin inhibitor heavy chain H5-like
protein
<400> 702
Met Ser Gly Trp Arg Tyr Leu Ile Cys Val Ser Phe Leu Leu Thr Ile
1 5 10 15
Leu Leu Glu Leu Thr Tyr Gln
20
<210> 703
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Inter-alpha-trypsin inhibitor heavy chain H4
<400> 703
Met Lys Pro Pro Arg Pro Val Arg Thr Cys Ser Lys Val Leu Val Leu
1 5 10 15
Leu Ser Leu Leu Ala Ile His Gln Thr Thr Thr Ala
20 25
<210> 704
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein KIAA0495
<400> 704
Met Cys Leu Leu Ser Ser Ser Ala Ala Ser Asp Leu Ala Ala Thr Ser
1 5 10 15
Leu Thr Ala
<210> 705
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plasma kallikrein
<400> 705
Met Ile Leu Phe Lys Gln Ala Thr Tyr Phe Ile Ser Leu Phe Ala Thr
1 5 10 15
Val Ser Cys
<210> 706
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig kappa chain V-I region HK101
<400> 706
Met Asp Met Arg Val Leu Ala Gln Leu Leu Gly Leu Leu Leu Leu Cys
1 5 10 15
Phe Pro Gly Ala Arg Cys
20
<210> 707
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Laminin subunit gamma-1
<400> 707
Met Arg Gly Ser His Arg Ala Ala Pro Ala Leu Arg Pro Arg Gly Arg
1 5 10 15
Leu Trp Pro Val Leu Ala Val Leu Ala Ala Ala Ala Ala Ala Gly Cys
20 25 30
Ala
<210> 708
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Left-right determination factor 2
<400> 708
Met Trp Pro Leu Trp Leu Cys Trp Ala Leu Trp Val Leu Pro Leu Ala
1 5 10 15
Gly Pro Gly Ala Ala
20
<210> 709
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lipase member H
<400> 709
Met Leu Arg Phe Tyr Leu Phe Ile Ser Leu Leu Cys Leu Ser Arg Ser
1 5 10 15
Asp Ala
<210> 710
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Noelin
<400> 710
Met Ser Val Pro Leu Leu Lys Ile Gly Val Val Leu Ser Thr Met Ala
1 5 10 15
<210> 711
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protocadherin alpha-10
<400> 711
Met Val Ser Arg Cys Ser Cys Leu Gly Val Gln Cys Leu Leu Leu Ser
1 5 10 15
Leu Leu Leu Leu Ala Ala Trp Glu Val Gly Ser Gly
20 25
<210> 712
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Plexin-B1
<400> 712
Met Pro Ala Leu Gly Pro Ala Leu Leu Gln Ala Leu Trp Ala Gly Trp
1 5 10 15
Val Leu Thr
<210> 713
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Prolactin
<400> 713
Met Asn Ile Lys Gly Ser Pro Trp Lys Gly Ser Leu Leu Leu Leu Leu
1 5 10 15
Val Ser Asn Leu Leu Leu Cys Gln Ser Val Ala Pro
20 25
<210> 714
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Poliovirus receptor-related protein 4
<400> 714
Met Pro Leu Ser Leu Gly Ala Glu Met Trp Gly Pro Glu Ala Trp Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Ala Ser Phe Thr Gly Arg Cys Pro Ala
20 25 30
<210> 715
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretoglobin family 1D member 4
<400> 715
Met Arg Leu Ser Val Cys Leu Leu Met Val Ser Leu Ala Leu Cys Cys
1 5 10 15
Tyr Gln Ala His Ala
20
<210> 716
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Slit homolog 2 protein
<400> 716
Met Arg Gly Val Gly Trp Gln Met Leu Ser Leu Ser Leu Gly Leu Val
1 5 10 15
Leu Ala Ile Leu Asn Lys Val Ala Pro Gln Ala Cys Pro Ala
20 25 30
<210> 717
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trefoil factor 1
<400> 717
Met Ala Thr Met Glu Asn Lys Val Ile Cys Ala Leu Val Leu Val Ser
1 5 10 15
Met Leu Ala Leu Gly Thr Leu Ala
20
<210> 718
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Scavenger receptor cysteine-rich domain-containing
protein LOC284297
<400> 718
Met Arg Val Leu Ala Cys Leu Leu Ala Ala Leu Val Gly Ile Gln Ala
1 5 10 15
<210> 719
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Trefoil factor 3
<400> 719
Met Ala Ala Arg Ala Leu Cys Met Leu Gly Leu Val Leu Ala Leu Leu
1 5 10 15
Ser Ser Ser Ser Ala
20
<210> 720
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Metalloproteinase inhibitor 1
<400> 720
Met Ala Pro Phe Glu Pro Leu Ala Ser Gly Ile Leu Leu Leu Leu Trp
1 5 10 15
Leu Ile Ala Pro Ser Arg Ala
20
<210> 721
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Vascular endothelial growth factor receptor 1
<400> 721
Met Val Ser Tyr Trp Asp Thr Gly Val Leu Leu Cys Ala Leu Leu Ser
1 5 10 15
Cys Leu Leu Leu Thr Gly Ser Ser Ser Gly
20 25
<210> 722
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide WNT1-inducible-signaling pathway protein 1
<400> 722
Met Arg Trp Phe Leu Pro Trp Thr Leu Ala Ala Val Thr Ala Ala Ala
1 5 10 15
Ala Ser Thr Val Leu Ala
20
<210> 723
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein ENSP00000244321
<400> 723
Met Arg Leu Ser Arg Arg Pro Glu Thr Phe Leu Leu Ala Phe Val Leu
1 5 10 15
Leu Cys Thr Leu Leu Gly Leu Gly Cys Pro
20 25
<210> 724
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Anthrax toxin receptor 2
<400> 724
Met Val Ala Glu Arg Ser Pro Ala Arg Ser Pro Gly Ser Trp Leu Phe
1 5 10 15
Pro Gly Leu Trp Leu Leu Val Leu Ser Gly Pro Gly Gly Leu Leu Arg
20 25 30
Ala
<210> 725
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Apolipoprotein C-I
<400> 725
Met Arg Leu Phe Leu Ser Leu Pro Val Leu Val Val Val Leu Ser Ile
1 5 10 15
Val Leu Glu Gly Pro Ala Pro Ala Gln Gly
20 25
<210> 726
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lymphotoxin-alpha
<400> 726
Met Thr Pro Pro Glu Arg Leu Phe Leu Pro Arg Val Cys Gly Thr Thr
1 5 10 15
Leu His Leu Leu Leu Leu Gly Leu Leu Leu Val Leu Leu Pro Gly Ala
20 25 30
Gln Gly
<210> 727
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAMTS-like protein 2
<400> 727
Met Asp Gly Arg Trp Gln Cys Ser Cys Trp Ala Trp Phe Leu Leu Val
1 5 10 15
Leu Ala Val Val Ala Gly
20
<210> 728
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAMTS-like protein 4
<400> 728
Met Glu Asn Trp Thr Gly Arg Pro Trp Leu Tyr Leu Leu Leu Leu Leu
1 5 10 15
Ser Leu Pro Gln Leu Cys Leu Asp
20
<210> 729
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement C1q-like protein 3
<400> 729
Met Val Leu Leu Leu Val Ile Leu Ile Pro Val Leu Val Ser Ser Ala
1 5 10 15
Gly Thr Ser Ala
20
<210> 730
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-6(IV) chain
<400> 730
Met Leu Ile Asn Lys Leu Trp Leu Leu Leu Val Thr Leu Cys Leu Thr
1 5 10 15
Glu Glu Leu Ala Ala Ala
20
<210> 731
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-6(VI) chain
<400> 731
Met Met Leu Leu Ile Leu Phe Leu Val Ile Ile Cys Ser His Ile Ser
1 5 10 15
Val Asn Gln
<210> 732
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EGF-like domain-containing protein 6
<400> 732
Met Pro Leu Pro Trp Ser Leu Ala Leu Pro Leu Leu Leu Ser Trp Val
1 5 10 15
Ala Gly Gly Phe Gly
20
<210> 733
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide EMILIN-3
<400> 733
Met Gly Arg Arg Arg Leu Leu Val Trp Leu Cys Ala Val Ala Ala Leu
1 5 10 15
Leu Ser Gly Ala Gln Ala
20
<210> 734
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ephrin type-A receptor 10
<400> 734
Met Glu Thr Cys Ala Gly Pro His Pro Leu Arg Leu Phe Leu Cys Arg
1 5 10 15
Met Gln Leu Cys Leu Ala Leu Leu Leu Gly Pro Trp Arg Pro Gly Thr
20 25 30
Ala
<210> 735
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibroblast growth factor-binding protein 3
<400> 735
Met Thr Pro Pro Lys Leu Arg Ala Ser Leu Ser Pro Ser Leu Leu Leu
1 5 10 15
Leu Leu Ser Gly Cys Leu Leu Ala Ala Ala
20 25
<210> 736
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement factor H-related protein 2
<400> 736
Met Trp Leu Leu Val Ser Val Ile Leu Ile Ser Arg Ile Ser Ser Val
1 5 10 15
Gly Gly
<210> 737
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibrinogen beta chain
<400> 737
Met Lys Arg Met Val Ser Trp Ser Phe His Lys Leu Lys Thr Met Lys
1 5 10 15
His Leu Leu Leu Leu Leu Leu Cys Val Phe Leu Val Lys Ser
20 25 30
<210> 738
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Fibronectin type III domain-containing protein 1
<400> 738
Met Ala Pro Glu Ala Gly Ala Thr Leu Arg Ala Pro Arg Arg Leu Ser
1 5 10 15
Trp Ala Ala Leu Leu Leu Leu Ala Ala Leu Leu Pro Val Ala Ser Ser
20 25 30
<210> 739
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Growth/differentiation factor 6
<400> 739
Met Asp Thr Pro Arg Val Leu Leu Ser Ala Val Phe Leu Ile Ser Phe
1 5 10 15
Leu Trp Asp Leu Pro Gly
20
<210> 740
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Hyaluronidase-1
<400> 740
Met Ala Ala His Leu Leu Pro Ile Cys Ala Leu Phe Leu Thr Leu Leu
1 5 10 15
Asp Met Ala Gln Gly
20
<210> 741
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interferon epsilon-1
<400> 741
Met Ile Ile Lys His Phe Phe Gly Thr Val Leu Val Leu Leu Ala Ser
1 5 10 15
Thr Thr Ile Phe Ser
20
<210> 742
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-1 receptor accessory protein
<400> 742
Met Thr Leu Leu Trp Cys Val Val Ser Leu Tyr Phe Tyr Gly Ile Leu
1 5 10 15
Gln Ser Asp Ala
20
<210> 743
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig kappa chain V-I region HK102
<400> 743
Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp
1 5 10 15
Leu Pro Gly Ala Lys Cys
20
<210> 744
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig kappa chain V-II region RPMI 6410
<400> 744
Met Arg Leu Pro Ala Gln Leu Leu Gly Leu Leu Met Leu Trp Val Pro
1 5 10 15
Gly Ser Ser Gly
20
<210> 745
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Epididymal-specific lipocalin-10
<400> 745
Met Arg Gln Gly Leu Leu Val Leu Ala Leu Val Leu Val Leu Val Leu
1 5 10 15
Val Leu Ala
<210> 746
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Leucine-rich repeat LGI family member 3
<400> 746
Met Ala Gly Leu Arg Ala Arg Gly Gly Pro Gly Pro Gly Leu Leu Ala
1 5 10 15
Leu Ser Ala Leu Gly Phe Cys Leu Met Leu Gln Val Ser Ala
20 25 30
<210> 747
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Lysozyme-like protein 6
<400> 747
Met Thr Lys Ala Leu Leu Ile Tyr Leu Val Ser Ser Phe Leu Ala Leu
1 5 10 15
Asn Gln Ala
<210> 748
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Mucin-like protein 1
<400> 748
Met Lys Phe Leu Ala Val Leu Val Leu Leu Gly Val Ser Ile Phe Leu
1 5 10 15
Val Ser Ala Gln
20
<210> 749
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Group IID secretory phospholipase A2
<400> 749
Met Glu Leu Ala Leu Leu Cys Gly Leu Val Val Met Ala Gly Val Ile
1 5 10 15
Pro Ile Gln Gly
20
<210> 750
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Procollagen C-endopeptidase enhancer 2
<400> 750
Met Arg Gly Ala Asn Ala Trp Ala Pro Leu Cys Leu Leu Leu Ala Ala
1 5 10 15
Ala Thr Gln Leu Ser Arg Gln
20
<210> 751
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Basic salivary proline-rich protein 1
<400> 751
Met Leu Leu Ile Leu Leu Ser Val Ala Leu Leu Ala Leu Ser Ser Ala
1 5 10 15
<210> 752
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Peroxidasin homolog
<400> 752
Met Ala Lys Arg Ser Arg Gly Pro Gly Arg Arg Cys Leu Leu Ala Leu
1 5 10 15
Val Leu Phe Cys Ala Trp Gly Thr Leu Ala
20 25
<210> 753
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ribonuclease 8
<400> 753
Met Ala Pro Ala Arg Ala Gly Cys Cys Pro Leu Leu Leu Leu Leu Leu
1 5 10 15
Gly Leu Trp Val Ala Glu Val Leu Val Arg Ala
20 25
<210> 754
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Semaphorin-3B
<400> 754
Met Gly Arg Ala Gly Ala Ala Ala Val Ile Pro Gly Leu Ala Leu Leu
1 5 10 15
Trp Ala Val Gly Leu Gly Ser Ala
20
<210> 755
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secreted Ly-6/uPAR-related protein 1
<400> 755
Met Ala Ser Arg Trp Ala Val Gln Leu Leu Leu Val Ala Ala Trp Ser
1 5 10 15
Met Gly Cys Gly Glu
20
<210> 756
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Tumor necrosis factor receptor superfamily member
6B
<400> 756
Met Arg Ala Leu Glu Gly Pro Gly Leu Ser Leu Leu Cys Leu Val Leu
1 5 10 15
Ala Leu Pro Ala Leu Leu Pro Val Pro Ala Val Arg Gly
20 25
<210> 757
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Triggering receptor expressed on myeloid cells 1
<400> 757
Met Arg Lys Thr Arg Leu Trp Gly Leu Leu Trp Met Leu Phe Val Ser
1 5 10 15
Glu Leu Arg Ala
20
<210> 758
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide von Willebrand factor
<400> 758
Met Ile Pro Ala Arg Phe Ala Gly Val Leu Leu Ala Leu Ala Leu Ile
1 5 10 15
Leu Pro Gly Thr Leu Cys
20
<210> 759
<211> 29
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein Wnt-16
<400> 759
Met Asp Arg Ala Ala Leu Leu Gly Leu Ala Arg Leu Cys Ala Leu Trp
1 5 10 15
Ala Ala Leu Leu Val Leu Phe Pro Tyr Gly Ala Gln Gly
20 25
<210> 760
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein FLJ21075
<400> 760
Met Gly Phe His Phe Cys Ile Trp Ile Ile Phe Leu Leu Pro Pro Pro
1 5 10 15
Cys Lys Lys Cys
20
<210> 761
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Arylsulfatase F
<400> 761
Met Arg Pro Arg Arg Pro Leu Val Phe Met Ser Leu Val Cys Ala Leu
1 5 10 15
Leu Asn Thr Cys Gln Ala
20
<210> 762
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide ADAMTS-like protein 5
<400> 762
Met Gly Lys Leu Arg Pro Gly Arg Val Glu Trp Leu Ala Ser Gly His
1 5 10 15
Thr Glu Arg Pro His Leu Phe Gln Asn Leu Leu Leu Phe Leu Trp Ala
20 25 30
<210> 763
<211> 27
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 17
<400> 763
Met Cys Asp Gly Ala Leu Leu Pro Pro Leu Val Leu Pro Val Leu Leu
1 5 10 15
Leu Leu Val Trp Gly Leu Asp Pro Gly Thr Ala
20 25
<210> 764
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide A disintegrin and metalloproteinase with
thrombospondin motifs 9
<400> 764
Met Gln Phe Val Ser Trp Ala Thr Leu Leu Thr Leu Leu Val Arg Asp
1 5 10 15
Leu Ala
<210> 765
<211> 23
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Bactericidal/permeability-increasing protein-like
2
<400> 765
Met Cys Thr Lys Thr Ile Pro Val Leu Trp Gly Cys Phe Leu Leu Trp
1 5 10 15
Asn Leu Tyr Val Ser Ser Ser
20
<210> 766
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein C1orf191
<400> 766
Met Trp Gly Phe Leu Val Leu Lys Ala Arg Trp Leu Val Thr Pro Val
1 5 10 15
Arg Thr
<210> 767
<211> 15
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Carboxypeptidase B
<400> 767
Met Leu Ala Leu Leu Val Leu Val Thr Val Ala Leu Ala Ser Ala
1 5 10 15
<210> 768
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Uncharacterized protein C10orf25
<400> 768
Met Val Pro Gly Pro Pro Glu Ser Val Val Arg Phe Phe Leu Trp Phe
1 5 10 15
Cys Phe Leu Leu Pro Pro Thr Arg Lys Ala Ser Cys
20 25
<210> 769
<211> 33
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide UPF0454 protein C12orf49
<400> 769
Met Val Asn Leu Ala Ala Met Val Trp Arg Arg Leu Leu Arg Lys Arg
1 5 10 15
Trp Val Leu Ala Leu Val Phe Gly Leu Ser Leu Val Tyr Phe Leu Ser
20 25 30
Ser
<210> 770
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative uncharacterized protein C14orf144
<400> 770
Met Cys Arg Glu Thr Ala Gly Tyr Gly Trp Leu Leu Ala Ser Thr Glu
1 5 10 15
Leu Leu Ser Leu Leu Glu Pro Leu Ser Pro
20 25
<210> 771
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Beta-Ala-His dipeptidase
<400> 771
Met Asp Pro Lys Leu Gly Arg Met Ala Ala Ser Leu Leu Ala Val Leu
1 5 10 15
Leu Leu Leu Leu Glu Arg Gly Met Phe Ser
20 25
<210> 772
<211> 25
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-2(IV) chain
<400> 772
Met Gly Arg Asp Gln Arg Ala Val Ala Gly Pro Ala Leu Arg Arg Trp
1 5 10 15
Leu Leu Leu Gly Thr Val Thr Val Gly
20 25
<210> 773
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement component C8 alpha chain
<400> 773
Met Phe Ala Val Val Phe Phe Ile Leu Ser Leu Met Thr Cys Gln Pro
1 5 10 15
Gly Val Thr Ala
20
<210> 774
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Collagen alpha-2(XI) chain
<400> 774
Met Glu Arg Cys Ser Arg Cys His Arg Leu Leu Leu Leu Leu Pro Leu
1 5 10 15
Val Leu Gly Leu Ser Ala
20
<210> 775
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Complement component receptor 1-like protein
<400> 775
Met Ala Pro Pro Val Arg Leu Glu Arg Pro Phe Pro Ser Arg Arg Phe
1 5 10 15
Pro Gly Leu Leu Leu Ala Ala Leu Val Leu Leu Leu Ser Ser Phe Ser
20 25 30
<210> 776
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Platelet basic protein
<400> 776
Met Ser Leu Arg Leu Asp Thr Thr Pro Ser Cys Asn Ser Ala Arg Pro
1 5 10 15
Leu His Ala Leu Gln Val Leu Leu Leu Leu Ser Leu Leu Leu Thr Ala
20 25 30
Leu Ala
<210> 777
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Follistatin-related protein 4
<400> 777
Met Lys Pro Gly Gly Phe Trp Leu His Leu Thr Leu Leu Gly Ala Ser
1 5 10 15
Leu Pro Ala Ala Leu Gly
20
<210> 778
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein G7c
<400> 778
Met Leu Pro Thr Glu Val Pro Gln Ser His Pro Gly Pro Ser Ala Leu
1 5 10 15
Leu Leu Leu Gln Leu Leu Leu Pro Pro Thr Ser Ala
20 25
<210> 779
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastric inhibitory polypeptide
<400> 779
Met Val Ala Thr Lys Thr Phe Ala Leu Leu Leu Leu Ser Leu Phe Leu
1 5 10 15
Ala Val Gly Leu Gly
20
<210> 780
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Gastrokine-1
<400> 780
Met Leu Ala Tyr Ser Ser Val His Cys Phe Arg Glu Asp Lys Met Lys
1 5 10 15
Phe Thr Ile Val
20
<210> 781
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glycosyltransferase 1 domain-containing protein 1
<400> 781
Met Arg Leu Leu Phe Leu Ala Val Leu Arg Pro His Thr Gly Asn Ala
1 5 10 15
<210> 782
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-20
<400> 782
Met Lys Ala Ser Ser Leu Ala Phe Ser Leu Leu Ser Ala Ala Phe Tyr
1 5 10 15
Leu Leu Trp Thr Pro Ser Thr Gly
20
<210> 783
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Interleukin-27 subunit beta
<400> 783
Met Thr Pro Gln Leu Leu Leu Ala Leu Val Leu Trp Ala Ser Cys Pro
1 5 10 15
Pro Cys Ser Gly
20
<210> 784
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kazal-type serine protease inhibitor
domain-containing protein 1
<400> 784
Met Leu Pro Pro Pro Arg Pro Ala Ala Ala Leu Ala Leu Pro Val Leu
1 5 10 15
Leu Leu Leu Leu Val Val Leu Thr Pro Pro Pro Thr Gly Ala
20 25 30
<210> 785
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-10
<400> 785
Met Arg Ala Pro His Leu His Leu Ser Ala Ala Ser Gly Ala Arg Ala
1 5 10 15
Leu Ala Lys Leu Leu Pro Leu Leu Met Ala Gln Leu Trp Ala
20 25 30
<210> 786
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Kallikrein-7
<400> 786
Met Ala Arg Ser Leu Leu Leu Pro Leu Gln Ile Leu Leu Leu Ser Leu
1 5 10 15
Ala Leu Glu Thr Ala Gly
20
<210> 787
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Ig kappa chain V region EV15
<400> 787
Met Gly Ser Gln Val His Leu Leu Ser Phe Leu Leu Leu Trp Ile Ser
1 5 10 15
Asp Thr Arg Ala
20
<210> 788
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Long palate, lung and nasal epithelium
carcinoma-associated protein 1
<400> 788
Met Ala Gly Pro Trp Thr Phe Thr Leu Leu Cys Gly Leu Leu Ala Ala
1 5 10 15
Thr Leu Ile Gln Ala
20
<210> 789
<211> 32
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Low-density lipoprotein receptor-related protein 8
<400> 789
Met Gly Leu Pro Glu Pro Gly Pro Leu Arg Leu Leu Ala Leu Leu Leu
1 5 10 15
Leu Leu Leu Leu Leu Leu Leu Leu Gln Leu Gln His Leu Ala Ala Ala
20 25 30
<210> 790
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Cartilage matrix protein
<400> 790
Met Arg Val Leu Ser Gly Thr Ser Leu Met Leu Cys Ser Leu Leu Leu
1 5 10 15
Leu Leu Gln Ala Leu Cys
20
<210> 791
<211> 24
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Melanoma-derived growth regulatory protein
<400> 791
Met Ala Arg Ser Leu Val Cys Leu Gly Val Ile Ile Leu Leu Ser Ala
1 5 10 15
Phe Ser Gly Pro Gly Val Arg Gly
20
<210> 792
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Matrix metalloproteinase-16
<400> 792
Met Ile Leu Leu Thr Phe Ser Thr Gly Arg Arg Leu Asp Phe Val His
1 5 10 15
His Ser Gly Val Phe Phe Leu Gln Thr Leu Leu Trp Ile Leu Cys
20 25 30
<210> 793
<211> 22
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Multimerin-2
<400> 793
Met Ile Leu Ser Leu Leu Phe Ser Leu Gly Gly Pro Leu Gly Trp Gly
1 5 10 15
Leu Leu Gly Ala Trp Ala
20
<210> 794
<211> 31
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Protein NOV homolog
<400> 794
Met Gln Ser Val Gln Ser Thr Ser Phe Cys Leu Arg Lys Gln Cys Leu
1 5 10 15
Cys Leu Thr Phe Leu Leu Leu His Leu Leu Gly Gln Val Ala Ala
20 25 30
<210> 795
<211> 26
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Oocyte-secreted protein 1 homolog
<400> 795
Met Lys Thr Ile Leu Gly Phe Lys Gly Leu Phe Tyr Leu His Ser Leu
1 5 10 15
Ile Trp Thr Cys Ala Gly Asp Trp Ser Ala
20 25
<210> 796
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Secretory phospholipase A2 receptor
<400> 796
Met Leu Leu Ser Pro Ser Leu Leu Leu Leu Leu Leu Leu Gly Ala Pro
1 5 10 15
Arg Gly Cys Ala
20
<210> 797
<211> 21
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Proline-rich protein 1
<400> 797
Met Lys Leu Thr Phe Phe Leu Gly Leu Leu Ala Leu Ile Ser Cys Phe
1 5 10 15
Thr Pro Ser Glu Ser
20
<210> 798
<211> 34
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Putative pregnancy-specific beta-1-glycoprotein 7
<400> 798
Met Gly Pro Leu Ser Ala Pro Pro Cys Thr Gln His Ile Thr Trp Lys
1 5 10 15
Gly Leu Leu Leu Thr Ala Ser Leu Leu Asn Phe Trp Asn Pro Pro Thr
20 25 30
Thr Ala
<210> 799
<211> 30
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Poliovirus receptor-related protein 1
<400> 799
Met Ala Arg Met Gly Leu Ala Gly Ala Ala Gly Arg Trp Trp Gly Leu
1 5 10 15
Ala Leu Gly Leu Thr Ala Phe Phe Leu Pro Gly Val His Ser
20 25 30
<210> 800
<211> 28
<212> PRT
<213> Artificial Sequence
<220>
<223> Signal peptide Glutaminyl-peptide cyclotransferase
<400> 800
Met Ala Gly Gly Arg His Arg Arg Val Val Gly Thr Leu His Leu Leu
1 5 10 15
Leu Leu Val Ala Ala Leu Pro Trp Ala Ser Arg Gly
20 25
<210> 801
<211> 6
<212> DNA
<213> Artificial Sequence
<220>
<223> Kozak sequence
<400> 801
gccacc 6
<210> 802
<211> 6
<212> DNA
<213> Artificial Sequence
<220>
<223> Kozak sequence
<400> 802
gccgcc 6
<210> 803
<211> 75
<212> DNA
<213> Artificial Sequence
<220>
<223> XhoI-GST Forward primer
<400> 803
gttactctcg agctggttcc gcgtggatct gactacaagg atgacgacga taagatgaga 60
tcccctatac taggt 75
<210> 804
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> ApaI Reverse primer
<400> 804
attagacggg cccgggtcag gatctttttg gaggatggtc gccacc 46
<210> 805
<211> 7920
<212> DNA
<213> Artificial Sequence
<220>
<223> pcDNA3.1(+)-Fluc-8XHis
<400> 805
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900
taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960
gcacagtggc ggccgccgcc ggcggccacc gggcgcgcca tggaggacgc caagaacatc 1020
aagaagggtc ccgccccctt ctaccccctg gaggacggta ccgccggtga gcagctgcac 1080
aaggccatga agagatacgc cctggtgccc ggtaccatcg ccttcaccga cgcccacatc 1140
gaggtgaaca tcacctacgc cgagtacttc gagatgagcg tgagactggc cgaggccatg 1200
aagagatacg gtctgaacac caaccacaga atcgtggtgt gcagcgagaa cagcctgcag 1260
ttcttcatgc ccgtgctggg tgccctgttc atcggtgtgg ccgtggcccc cgccaacgac 1320
atctacaacg agagagagct gctgaacagc atgaacatca gccagcccac cgtggtgttc 1380
gtgagcaaga agggtctgca gaagatcctg aacgtgcaga agaagctgcc catcatccag 1440
aagatcatca tcatggacag caagaccgac taccagggtt tccagagcat gtacaccttc 1500
gtgaccagcc acctgccccc cggtttcaac gagtacgact tcgtgcccga gagcttcgac 1560
agagacaaga ccatcgccct gatcatgaac agcagcggta gcaccggtct gcccaagggt 1620
gtggccctgc cccacagaac cgcctgcgtg agattcagcc acgccagaga ccccatcttc 1680
ggtaaccaga tcatccccga caccgccatc ctgagcgtgg tgcccttcca ccacggtttc 1740
ggtatgttca ccaccctggg ttacctgatc tgcggtttca gagtggtgct gatgtacaga 1800
ttcgaggagg agctgttcct gagaagcctg caggactaca agatccagag cgccctgctg 1860
gtgcccaccc tgttcagctt cttcgccaag agcaccctga tcgacaagta cgacctgagc 1920
aacctgcacg agatcgccag cggtggtgcc cccctgagca aggaggtggg tgaggccgtg 1980
gccaagagat tccacctgcc cggtatcaga cagggttacg gtctgaccga gaccaccagc 2040
gccatcctga tcacccccga gggtgacgac aagcccggtg ccgtgggtaa ggtggtgccc 2100
ttcttcgagg ccaaggtggt ggacctggac accggtaaga ccctgggtgt gaaccagaga 2160
ggtgagctgt gcgtgagagg tcccatgatc atgagcggtt acgtgaacaa ccccgaggcc 2220
accaacgccc tgatcgacaa ggacggttgg ctgcacagcg gtgacatcgc ctactgggac 2280
gaggacgagc acttcttcat cgtggacaga ctgaagagcc tgatcaagta caagggttac 2340
caggtggccc ccgccgagct ggagagcatc ctgctgcagc accccaacat cttcgacgcc 2400
ggtgtggccg gtctgcccga cgacgacgcc ggtgagctgc ccgccgccgt ggtggtgctg 2460
gagcacggta agaccatgac cgagaaggag atcgtggact acgtggccag ccaggtgacc 2520
accgccaaga agctgagagg tggtgtggtg ttcgtggacg aggtgcccaa gggtctgacc 2580
ggtaagctgg acgccagaaa gatcagagag atcctgatca aggccaagaa gggtggtaag 2640
agcaagctgc accaccacca ccaccaccac cactaacgcg cgcgctcgag ctggttccgc 2700
gtggatctga ctacaaggat gacgacgata agatgagatc ccctatacta ggttattgga 2760
aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg 2820
aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg 2880
gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta 2940
tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc 3000
gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa 3060
ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa 3120
tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa 3180
cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt 3240
gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa 3300
ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca 3360
cgtttggtgg tggcgaccat cctccaaaaa gatcctgacc cgggcccgcg gttcgaaggt 3420
aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat 3480
caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 3540
gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 3600
tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 3660
ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 3720
gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 3780
ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 3840
accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 3900
gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 3960
tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 4020
gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 4080
agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 4140
ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 4200
tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 4260
ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 4320
agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 4380
ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 4440
ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 4500
ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 4560
tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 4620
gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 4680
tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 4740
cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 4800
tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 4860
tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 4920
aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 4980
tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 5040
gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 5100
aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 5160
acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 5220
atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 5280
acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 5340
tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 5400
ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc 5460
aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 5520
atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 5580
ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 5640
acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 5700
atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 5760
tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 5820
gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 5880
gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 5940
atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 6000
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 6060
gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 6120
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 6180
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 6240
ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 6300
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 6360
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 6420
cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 6480
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 6540
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 6600
agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 6660
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 6720
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 6780
tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 6840
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 6900
tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 6960
atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 7020
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 7080
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 7140
gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 7200
tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 7260
tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 7320
tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 7380
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 7440
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 7500
tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 7560
catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 7620
aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 7680
tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 7740
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 7800
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 7860
tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 7920
7920
<210> 806
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_704_first oligonucleotide
<400> 806
ggccgccgcc ggcggccacc atgtgcctgc 30
<210> 807
<211> 50
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_704_second oligonucleotide
<400> 807
ggtcgctggc ggcgctgctg ctcagcaggc acatggtggc cgccggcggc 50
<210> 808
<211> 50
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_704_third oligonucleotide
<400> 808
tgagcagcag cgccgccagc gacctggccg ccaccagcct gaccgccggg 50
<210> 809
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_704_fourth oligonucleotide
<400> 809
cgcgcccggc ggtcaggctg gtggcggcca 30
<210> 810
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_first oligonucleotide
<400> 810
ggccgccgcc ggcggccacc atgtgcttcc ccaaggtgct gagcgacg 48
<210> 811
<211> 65
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_second oligonucleotide
<400> 811
gggccttcag cttcttcatg tcgtcgctca gcaccttggg gaagcacatg gtggccgccg 60
gcggc 65
<210> 812
<211> 65
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_third oligonucleotide
<400> 812
acatgaagaa gctgaaggcc cgcatggtga tgctgctgcc caccagcgcc cagggcctgg 60
gcggg 65
<210> 813
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_fourth oligonucleotide
<400> 813
cgcgcccgcc caggccctgg gcgctggtgg gcagcagcat caccatgc 48
<210> 814
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_first oligonucleotide
<400> 814
ggccgccgcc ggcggccacc atgcgcggca gccaccgcgc cgccccc 47
<210> 815
<211> 75
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_second oligonucleotide
<400> 815
acgggccaca ggcggccacg ggggcgcagg gcgggggcgg cgcggtggct gccgcgcatg 60
gtggccgccg gcggc 75
<210> 816
<211> 75
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_third oligonucleotide
<400> 816
gccctgcgcc cccgtggccg cctgtggccc gtgctggccg tgctggccgc cgccgccgcc 60
gccggctgcg ccggg 75
<210> 817
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_fourth oligonucleotide
<400> 817
cgcgcccggc gcagccggcg gcggcggcgg cggccagcac ggccagc 47
<210> 818
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_first oligonucleotide
<400> 818
ggccgccgcc ggcggccacc atgtgcttcc ccaag 35
<210> 819
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_second oligonucleotide
<400> 819
gctcagcacc ttggggaagc acatggtggc cgccggcggc 40
<210> 820
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_third oligonucleotide
<400> 820
gtgctgagcg acgacatgaa gaagctgaag gcccgcatgg tgatg 45
<210> 821
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_fourth oligonucleotide
<400> 821
gggcagcagc atcaccatgc gggccttcag cttcttcatg tcgtc 45
<210> 822
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_fifth oligonucleotide
<400> 822
ctgctgccca ccagcgccca gggcctgggc ggg 33
<210> 823
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_701_sixth oligonucleotide
<400> 823
cgcgcccgcc caggccctgg gcgctggt 28
<210> 824
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_first oligonucleotide
<400> 824
ggccgccgcc ggcggccacc atgcgcggca gccaccgcgc 40
<210> 825
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_second oligonucleotide
<400> 825
gcgggggcgg cgcggtggct gccgcgcatg gtggccgccg gcggc 45
<210> 826
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_third oligonucleotide
<400> 826
cgcccccgcc ctgcgccccc gtggccgcct gt 32
<210> 827
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_fourth oligonucleotide
<400> 827
gcacgggcca caggcggcca cgggggcgca gg 32
<210> 828
<211> 50
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_fifth oligonucleotide
<400> 828
ggcccgtgct ggccgtgctg gccgccgccg ccgccgccgg ctgcgccggg 50
<210> 829
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> Signal peptide_707_sixth oligonucleotide
<400> 829
cgcgcccggc gcagccggcg gcggcggcgg cggccagcac ggcca 45
Claims (10)
- 5'에서 3' 방향으로 프로모터, 제1 제한효소 인식 부위, 코작 서열, 시그널 펩타이드 암호화 서열, 제2 제한효소 인식 부위 및 poly A 서열을 순차적으로 포함하는, 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터로서,
상기 발현 벡터는 서열번호 805로 이루어진 pcDNA3.1(+)-Fluc-8XHis 벡터의 NotⅠ 및 AscⅠ 제한효소 인식 부위 사이에 코작 서열 및 시그널 펩타이드 암호화 서열이 순차적으로 삽입되고,
상기 시그널 펩타이드 암호화 서열은 서열번호 1 내지 800으로 이루어진 군으로부터 선택되는 어느 하나로 기재되는 아미노산 서열을 암호화하는, 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터.
- 삭제
- 삭제
- 삭제
- 삭제
- 제 1항에 있어서,
목적 단백질을 암호화하는 유전자는 상기 시그널 펩타이드 암호화 서열의 하류(downstream) 및 poly A 서열 사이에 도입되는 것을 특징으로 하는, 목적 단백질을 세포 외로 분비시키기 위한 발현 벡터.
- 삭제
- 삭제
- 목적 단백질을 세포 외로 분비시키기 위한 제 1항의 벡터들을 포함하는 발현 벡터 라이브러리.
- 1) 제 9항의 라이브러리에 포함된 2 내지 800 종류의 발현 벡터에 목적 단백질을 암호화하는 유전자를 삽입하는 단계;
2) 상기 단계 1)의 목적 단백질을 암호화하는 유전자가 삽입된 발현 벡터를 각각 숙주세포에 형질도입(transient transfection)하는 단계; 및
3) 상기 단계 2)의 형질도입된 숙주세포의 배양액으로부터 목적 단백질을 정량하는 단계를 포함하는, 목적 단백질을 세포 외로 대량으로 분비시킬 수 있는 시그널 펩타이드의 탐색 방법.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021045541A1 (ko) * | 2019-09-03 | 2021-03-11 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
| KR20210028116A (ko) * | 2019-09-03 | 2021-03-11 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021045541A1 (ko) * | 2019-09-03 | 2021-03-11 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
| KR20210028116A (ko) * | 2019-09-03 | 2021-03-11 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
| KR102406463B1 (ko) | 2019-09-03 | 2022-06-10 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
| CN114651065A (zh) * | 2019-09-03 | 2022-06-21 | 五松尖端医疗产业振兴财团 | 导入用于提高蛋白质生产性的单个条形码系统的信号肽超高速筛选方法 |
| JP2022546775A (ja) * | 2019-09-03 | 2022-11-08 | オソン メディカル イノベーション ファウンデーション | タンパク質生産性の向上のための個別バーコーディングシステムを導入したシグナルペプチド超高速選別方法 |
| JP7357771B2 (ja) | 2019-09-03 | 2023-10-06 | オソン メディカル イノベーション ファウンデーション | タンパク質生産性の向上のための個別バーコーディングシステムを導入したシグナルペプチド超高速選別方法 |
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