CN117327173A - 广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用 - Google Patents

广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用 Download PDF

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CN117327173A
CN117327173A CN202210724658.1A CN202210724658A CN117327173A CN 117327173 A CN117327173 A CN 117327173A CN 202210724658 A CN202210724658 A CN 202210724658A CN 117327173 A CN117327173 A CN 117327173A
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张林琦
李明茜
雷雨晴
洪俊贤
杨依凌
史宣玲
张绮
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Tsinghua University
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Abstract

本发明公开了广谱中和SARS‑CoV‑2以及其他沙贝病毒的抗体及其应用。本发明提供了4种抗体(纳米抗体)。本发明保护4种抗体中的任意一种或任意组合。所述4种抗体为：序列8所示的TH‑34抗体、序列10所示的TH‑41抗体、序列12所示的TH‑44抗体和序列14所示的TH‑40抗体。本发明提供的抗体具有广谱中和SARS‑CoV‑2的效果，对野生型新型冠状病毒以及自然变异株均具有强中和能力，同时对其他沙贝病毒亚属的冠状病毒也具有强中和能力。本发明对于新型冠状病毒和/或其他沙贝病毒的防控具有重大的应用价值，将产生深远的社会意义。

Description

广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用
技术领域
本发明属于生物技术领域，涉及广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用，具体的所述抗体可为纳米抗体或纳米抗体-Fc融合抗体。
背景技术
新型冠状病毒(SARS-CoV-2，Severe Acute Respiratory Syndrome Coronavirus2)属于β属冠状病毒中的沙贝病毒(Sarbecovirus)亚属。随着传播进程，SARS-CoV-2产生了许多突变，导致新变异株的传播能力不断增强，并产生了不同程度的免疫逃逸，主要流行变异株例如Alpha株、Beta株、Gamma株、Delta株和Omicron株等。变异株的出现导致疫苗的保护效力产生不同程度的下降，许多单克隆抗体也对突变株的中和活性减弱甚至完全失去中和活性。
除了SARS-CoV-2以外，严重急性呼吸综合征冠状病毒(SARS-CoV-1,Severe AcuteRespiratory Syndrome Coronavirus 1)也属于沙贝病毒亚属。沙贝病毒亚属常以蝙蝠作为动物宿主，可进一步划分为3个分支：第一类，感染人类的SARS-CoV-1病毒和与其基因组相似的蝙蝠衍生SARS-CoV-1病毒株，例如Bat SARS-like WIV1等；第二类，蝙蝠衍生SARS-CoV-1样病毒株，例如Bat SARS-like CoV ZC45和Bat SARS-like CoV ZXC21等；第三类，感染人类的SARS-CoV-2病毒和与其基因组相似的蝙蝠衍生和穿山甲衍生病毒株，例如BatCoV RaTG13等。二十世纪以来，沙贝病毒已在人类世界中引起了两次重大疫情(SARS-CoV-1和SARS-CoV-2)，研究者也不断地从自然宿主中发现更多的与SARS-CoV-1接近的沙贝病毒。为了应对未来的变异株和可能由自然宿主进化感染人类的沙贝病毒，发现预防和治疗沙贝病毒感染的通用药物和通用疫苗，成为结束疫情大流行的关键，也是防止未来更多变异和可能的新冠状病毒爆发的关键。
常规免疫球蛋白IgG抗体由两条相同的重链(Heavy chain)和两条相同的轻链(Light chain)组成，例如分离自人体的单克隆抗体或人源化小鼠抗体。除了常规抗体外，骆驼科动物体内还存在独特的重链抗体(HCAbs)，仅由两条相同的重链组成，每个重链由1个重链可变区(VHH)和两个恒定区(CH2和CH3)组成。VHH是重链抗体最小的完整功能结构，也称为纳米抗体(Nanobody)或单域抗体。纳米抗体具有体积小、特异性强、稳定性强、易生产、穿透力强、免疫原性低等多种优势。得益于纳米抗体的小体积和高特异性，目标蛋白上众多较为隐蔽的抗体表位更容易被纳米抗体识别，有利于进一步分辨得到更多中和抗体表位，为抗体药物和疫苗研发等提供创新的思路，为疫情防控提供有效的技术和方法。
发明内容
本发明的目的是提供广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用。
本发明提供了4种抗体(纳米抗体)。本发明保护4种抗体中的任意一种或任意组合。
所述4种抗体为：TH-34抗体、TH-41抗体、TH-44抗体和TH-40抗体。
本发明还提供了4种抗体(融合抗体)。本发明保护4种抗体中的任意一种或任意组合。
所述4种抗体为：TH-34-Fc抗体、TH-41-Fc抗体、TH-44-Fc抗体和TH-40-Fc抗体。
所述TH-34抗体包括框架区FR和互补决定区CDR；所述TH-34抗体中的CDR1、CDR2和CDR3依次为序列表的序列8中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-34抗体中的FR1、FR2、FR3和FR4依次为序列表的序列8中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基。
所述TH-41抗体包括框架区FR和互补决定区CDR；所述TH-41抗体中的CDR1、CDR2和CDR3依次为序列表的序列10中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-41抗体中的FR1、FR2、FR3和FR4依次为序列表的序列10中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基。
所述TH-44抗体包括框架区FR和互补决定区CDR；所述TH-44抗体中的CDR1、CDR2和CDR3依次为序列表的序列12中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-44抗体中的FR1、FR2、FR3和FR4依次为序列表的序列12中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基。
所述TH-40抗体包括框架区FR和互补决定区CDR；所述TH-40抗体中的CDR1、CDR2和CDR3依次为序列表的序列14中第26-33位、第51-58位、第97-115位氨基酸残基；所述TH-40抗体中的FR1、FR2、FR3和FR4依次为序列表的序列14中第1-25位、第34-50位、第59-96位、第116-126位氨基酸残基。
具体的，所述TH-34抗体如序列表的序列8所示。
具体的，所述TH-41抗体如序列表的序列10所示。
具体的，所述TH-44抗体如序列表的序列12所示。
具体的，所述TH-40抗体如序列表的序列14所示。
具体的，所述TH-34抗体自N端至C端包括如下区段：序列表的序列8所示蛋白质区段，蛋白质标签。具体的，所述TH-34抗体自N端至C端依次由如下区段组成：序列表的序列8所示蛋白质区段，蛋白质标签。
具体的，所述TH-41抗体自N端至C端包括如下区段：序列表的序列10所示蛋白质区段，蛋白质标签。具体的，所述TH-41抗体自N端至C端依次由如下区段组成：序列表的序列10所示蛋白质区段，蛋白质标签。
具体的，所述TH-44抗体自N端至C端包括如下区段：序列表的序列12所示蛋白质区段，蛋白质标签。具体的，所述TH-44抗体自N端至C端依次由如下区段组成：序列表的序列12所示蛋白质区段，蛋白质标签。
具体的，所述TH-40抗体自N端至C端包括如下区段：序列表的序列14所示蛋白质区段，蛋白质标签。具体的，所述TH-40抗体自N端至C端依次由如下区段组成：序列表的序列14所示蛋白质区段，蛋白质标签。
具体的，所述蛋白质标签可为His6标签。
所述TH-34-Fc抗体自N端至C端包括如下区段：TH-34抗体、Fc。
所述TH-41-Fc抗体自N端至C端包括如下区段：TH-41抗体、Fc。
所述TH-44-Fc抗体自N端至C端包括如下区段：TH-44抗体、Fc。
所述TH-40-Fc抗体自N端至C端包括如下区段：TH-40抗体、Fc。
具体的，所述TH-34-Fc抗体自N端至C端依次包括如下区段：序列表的序列8所示蛋白质区段，连接肽，Fc。具体的，所述TH-34-Fc抗体自N端至C端依次由如下区段组成：序列表的序列8所示蛋白质区段，连接肽，Fc。
具体的，所述TH-41-Fc抗体自N端至C端依次包括如下区段：序列表的序列10所示蛋白质区段，连接肽，Fc。具体的，所述TH-41-Fc抗体自N端至C端依次由如下区段组成：序列表的序列10所示蛋白质区段，连接肽，Fc。
具体的，所述TH-44-Fc抗体自N端至C端依次包括如下区段：序列表的序列12所示蛋白质区段，连接肽，Fc。具体的，所述TH-44-Fc抗体自N端至C端依次由如下区段组成：序列表的序列12所示蛋白质区段，连接肽，Fc。
具体的，所述TH-40-Fc抗体自N端至C端依次包括如下区段：序列表的序列14所示蛋白质区段，连接肽，Fc。具体的，所述TH-40-Fc抗体自N端至C端依次由如下区段组成：序列表的序列14所示蛋白质区段，连接肽，Fc。
所述连接肽具体可为“GGGGS”。
所述Fc具体可为人源Fc。
具体的，所述人源Fc如序列表的序列32所示。
本发明还提供了4种基因。本发明保护4种基因中的任意一种或任意组合。
所述4种基因为：编码TH-34抗体的基因、编码TH-41抗体的基因、编码TH-44抗体的基因、编码TH-40抗体的基因。
本发明还提供了4种基因。本发明保护4种基因中的任意一种或任意组合。
所述4种基因为：编码TH-34-Fc抗体的基因、编码TH-41-Fc抗体的基因、编码TH-44-Fc抗体的基因、编码TH-40-Fc抗体的基因。
本发明保护以上任一所述抗体或抗体组合在制备用于抑制冠状病毒的药物中的应用。
本发明还保护一种用于抑制冠状病毒的药物，其活性成分为以上任一所述抗体或抗体组合。
本发明保护以上任一所述抗体或抗体组合在制备用于中和冠状病毒的药物中的应用。
本发明还保护一种用于中和冠状病毒的药物，其活性成分为以上任一所述抗体或抗体组合。
本发明保护以上任一所述抗体或抗体组合在制备用于预防和/或治疗冠状病毒引起的疾病的药物中的应用。
本发明还保护一种用于预防和/或治疗冠状病毒引起的疾病的药物，其活性成分为以上任一所述抗体或抗体组合。
具体的，所述冠状病毒为β属冠状病毒。
具体的，所述冠状病毒为沙贝病毒亚属病毒。
具体的，所述冠状病毒为新型冠状病毒(SARS-CoV-2)。
具体的，所述新型冠状病毒可为如下任一：野生型新型冠状病毒、新型冠状病毒Alpha株、新型冠状病毒Beta株、新型冠状病毒Gamma株、新型冠状病毒Delta株、新型冠状病毒Omicron BA.1株、新型冠状病毒Omicron BA.2株。
具体的，所述冠状病毒为严重急性呼吸综合征冠状病毒(SARS-CoV-1)。
具体的，所述沙贝病毒亚属病毒为Pangolin CoV GD、Pangolin CoV GX、Bat CoVWIV16或Bat CoV RaTG13。
本发明利用SARS-CoV-2的spike蛋白和SARS-CoV-1的spike蛋白作为钓饵，从免疫后的羊驼体内分离获得具同时具有对SARS-CoV-2和SARS-CoV-1的spike蛋白具有结合能力的4种纳米抗体，命名为TH-34纳米抗体、TH-41纳米抗体、TH-44纳米抗体、TH-40纳米抗体。进一步，本发明还获得了4种纳米抗体和人源Fc融合的相应的4种融合抗体。本发明提供的抗体具有广谱中和SARS-CoV-2的效果，对野生型新型冠状病毒以及自然变异株均具有强中和能力，同时对其他沙贝病毒亚属的冠状病毒也具有强中和能力。本发明对于新型冠状病毒和/或其他沙贝病毒的防控具有重大的应用价值，将产生深远的社会意义。
附图说明
图1为SARS-CoV-2RBD蛋白纯化过程中的凝胶过滤层析色谱图。
图2为SARS-CoV-2S蛋白纯化过程中的凝胶过滤层析色谱图。
图3为SARS-CoV-1S蛋白纯化过程中的凝胶过滤层析色谱图。
图4为4种纳米抗体对野生型新型冠状病毒假病毒的中和试验结果。
图5为4种纳米抗体对SARS-CoV-1假病毒的中和试验结果。
具体实施方式
下面结合具体实施方式对本发明进行进一步的详细描述，给出的实施例仅为了阐明本发明，而不是为了限制本发明的范围。以下提供的实施例可作为本技术领域普通技术人员进行进一步改进的指南，并不以任何方式构成对本发明的限制。
下述实施例中的实验方法，如无特殊说明，均为常规方法，按照本领域内的文献所描述的技术或条件或者按照产品说明书进行。实施例中的重组质粒均已进行测序验证。如无特殊说明，以下实施例中的定量试验，均设置三次重复实验，结果取平均值。下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。293F细胞：Thermo FisherScientific,R79007。质粒pFastBac-dual：Gibco，10712024。质粒pcDNA3.1(+)：Invitrogen公司，产品目录号V790-20。293T细胞：盖德，CRL-11268。昆虫细胞培养基(Sf-900II SFM)：Gibco.，货号为10902-088。腺病毒疫苗AdC68-19S，即专利申请202010369075.2(申请公布号CN113583978A，申请公布日2021.11.02)中制备的ChAdTS-COVID-19S病毒液。pMD18-T载体为Takara货号6011的pMDTM 18-T Vector Cloning Kit的组件，https://www.takarabiomed.com.cn/ProductShow.aspx？m＝20141220150817403017&productI D＝20141227130215047304。
hACE2-hela细胞(即文献中的“HeLa cell lines stably expressing the ACE2molecules”)，记载于如下文献：Wang,R.,Zhang,Q.,Ge,J.,Ren,W.,Zhang,R.,Lan,J.,Ju,B.,Su,B.,Yu,F.,Chen,P.,Liao,H.,Feng,Y.,Li,X.,Shi,X.,Zhang,Z.,Zhang,F.,Ding,Q.,Zhang,T.,Wang,X.&Zhang,L.Analysis of SARS-CoV-2variant mutations revealsneutralization escape mechanisms and the ability to use ACE2 receptors fromadditional species.Immunity 54,1611-1621.e1615,doi:10.1016/j.immuni.2021.06.003(2021).。
pVRC8400载体(即文献中的pVRC8400 expression vector)记载于如下文献：LukeH Chao,Daryl E Klein,Aaron G Schmidt,Jennifer MStephen C Harrison.；Sequential conformational rearrangements in flavivirus membrane fusion；eLife,2014；3:e04389。
实施例1、纳米抗体的筛选
一、免疫原蛋白的制备
1、SARS-CoV-2RBD蛋白的制备
将质粒pcDNA3.1(+)的BamHI和HindIII酶切位点之间的小片段替换为序列表的序列2所示的双链DNA分子，得到重组质粒pcDNA3.1-SARS-CoV-2RBD。
序列表的序列2所示的DNA分子编码序列表的序列1所示的蛋白质。将序列表的序列1所示的蛋白质命名为SARS-CoV-2RBD蛋白。SARS-CoV-2RBD蛋白以二聚体形式存在，二聚体的预期分子量约为50kDa。
序列表的序列1中，第1-33位氨基酸残基组成信号肽，第34-256位氨基酸残基组成SARS-CoV-2RBD，第257-264位氨基酸残基组成strep标签，第265-272位氨基酸残基组成Flag标签，第273-278位氨基酸残基组成His6标签。
将重组质粒pcDNA3.1-SARS-CoV-2RBD转染293F细胞，采用SMM 293-TII培养基培养72h，然后4000rpm离心30min，收集含SARS-CoV-2RBD蛋白的上清液。
2、SARS-CoV-2S蛋白的制备
将质粒pcDNA3.1(+)的BamHI和HindIII酶切位点之间的小片段替换为序列表的序列4所示的双链DNA分子，得到重组质粒pcDNA3.1-SARS-CoV-2spike 2P。
序列表的序列4所示的DNA分子编码序列表的序列3所示的蛋白质。将序列表的序列3所示的蛋白质命名为SARS-CoV-2spike 2P胞外区融合蛋白，简称SARS-CoV-2S蛋白。SARS-CoV-2S蛋白以三聚体形式存在，三聚体的预期分子量为420kDa。
序列表的序列3中，第1-13位氨基酸残基组成信号肽，第14-1211位氨基酸残基组成SARS-CoV-2胞外区，第1212-1219位氨基酸残基组成3C酶切位点，第1220-1227位氨基酸残基组成连接肽，第1228-1255位氨基酸残基组成三聚体标签(trimer tag，功能为促使形成稳定三聚体)，第1256-1263位氨基酸残基组成strep标签，第1264-1269位氨基酸残基组成His6标签。
野生型新型冠状病毒中的相应蛋白如序列表的序列5所示。与序列5所示蛋白相比，序列3所示蛋白进行了如下改造：引入了两个突变，即S1/S2酶切位点由“RRKR”突变为“GSAS”，并且“KV”突变为“PP”，以增加蛋白稳定性；C端引入了3C酶切位点、连接肽、三聚体标签、strep标签和His6标签。
将重组质粒pcDNA3.1-SARS-CoV-2spike 2P转染293F细胞，采用SMM 293-TII培养基培养72h，然后4000rpm离心30min，收集含SARS-CoV-2S蛋白的上清液。
3、SARS-CoV-1S蛋白的制备
(1)构建重组质粒
将质粒pFastBac-dual的EcoRI和XbaI酶切位点之间的小片段替换为序列表的序列7所示的双链DNA分子，得到重组质粒pFastBac-SARS-CoV-1spike。
序列表的序列7所示的DNA分子编码序列表的序列6所示的蛋白质。将序列表的序列6所示的蛋白质命名为SARS-CoV-1spike胞外区融合蛋白，简称SARS-CoV-1S蛋白。SARS-CoV-1S蛋白以三聚体形式存在，三聚体的预期分子量为420kDa。
序列表的序列6中，第1-13位氨基酸残基组成信号肽，第14-1195位氨基酸残基组成SARS-CoV-1胞外区，第1196-1203位氨基酸残基组成3C酶切位点，第1204-1211位氨基酸残基组成连接肽，第1212-1239位氨基酸残基组成三聚体标签(trimer tag，功能为促使形成稳定三聚体)，第1240-1245位氨基酸残基组成His6标签，第1246-1253位氨基酸残基组成strep标签。
(2)重组Bacmid的制备
①将重组质粒pFastBac-SARS-CoV-1spike加入大肠杆菌DH10 Bac感受态细胞中，冰上放置30min；然后42℃热激75s，放回冰上2min；然后加入500μl LB液体培养基，37℃复苏5h；然后吸取10μl涂布于含50μg/ml卡那霉素、7μg/ml庆大霉素、10μg/ml四环素、40μg/mlIPTG和100μg/ml X-gal的LB固体培养基，避光培养三天至长出清晰的蓝白斑。
②挑取白色单菌落，接种于5mL含50μg/ml卡那霉素、7μg/ml庆大霉素、10μg/ml四环素的LB液体培养基，37℃、220rpm振荡培养12小时。
③取步骤②得到的培养体系，采用的质粒小提试剂盒(QIAprep Spin MiniprepKit，Qiagen公司，货号为27106,其中含有P1试剂、P2试剂和P3试剂)提取质粒，具体步骤依次如下：将培养体系13000rpm离心2min，收集菌体沉淀，用P1试剂重悬菌体；加入P2试剂，缓慢颠倒混匀6-8次；加入P3试剂，缓慢颠倒混匀6-8次(可见白色沉淀)，13000rpm离心10min，取上清液；取600μl上清液，加入800μl预冷的异丙醇，-20℃放置10min，然后13000rpm离心15min，收集沉淀；用500μl预冷的70％乙醇水溶液重悬沉淀，13000rpm离心5min，收集沉淀，将酒精完全吹干后，用65℃预热的ddH2O溶解沉淀，13000rpm离心5min，吸取上清，即为重组Bacmid的溶液，简称Bacmid溶液。
(3)重组病毒的制备与扩增
①取Sf9细胞，加入10cm培养皿中，静置10min，细胞贴壁，显微镜下观察，保证培养皿底部约有70％-80％被细胞覆盖。
②取Cellfectin II Reagent 15μl，用100μl昆虫细胞培养基稀释。
③取15-20μl步骤(2)得到的Bacmid溶液，用100μl昆虫细胞培养基稀释。
④将步骤②得到的液相缓慢加入步骤③得到的液相中，缓慢吹打均匀，室温静置30min，用昆虫细胞培养基稀释至2ml。
⑤取完成步骤①的培养皿，弃去上清，缓慢均匀的将步骤④得到的液相滴加至培养皿中，27℃静置培养5h后吸弃上清，加入7ml新鲜的昆虫细胞培养基，用封口膜密封后27℃静置培养8天，收集培养液，600g离心6min，取上清液，加入胎牛血清并使其体积浓度为2-5％，长期保存，即为P0代重组病毒的病毒液。
⑥取P0代重组病毒的病毒液，按照1:1000的体积比加入摇瓶培养的细胞浓度为2×106个细胞/mL的Sf9细胞液中，27℃、110rpm培养5天，收集培养液，600g离心6min，取上清液，即为P1代重组病毒的病毒液，简称P1代病毒液。
(4)蛋白的表达
取P1代病毒液，按照1:100的体积比加入1L细胞浓度为2×106个细胞/mL的Sf9细胞液中，27℃、125rpm培养72小时，4000rpm离心15min，收集上清液，即为含SARS-CoV-1S蛋白的上清液。
4、蛋白的纯化
待纯化蛋白液分别为：步骤1制备的含SARS-CoV-2RBD蛋白的上清液或者步骤2制备的含SARS-CoV-2S蛋白的上清液或者步骤3制备的含SARS-CoV-1S蛋白的上清液。
(1)亲和层析
亲和层析的层析柱规格：长度3cm，内径1cm。
亲和层析的柱填料：镍柱beads(购自Qiagen公司，产品目录号为30230)。
依次进行如下操作步骤：①将500ml待纯化蛋白液上样于亲和层析柱，4℃孵育3小时；②用100mL含20mM咪唑的HEPEs buffer(pH7.2、1M)洗涤柱子；③用30mL含500mM咪唑的HEPEs buffer(pH7.2、1M)洗脱目的蛋白，收集过柱后溶液。
(2)取亲和层析后得到的过柱后溶液，用10kD浓缩管(购自Merck公司，产品目录号为UFC800396)进行浓缩，得到体积为1mL的浓缩液。
(3)凝胶过滤层析
凝胶过滤层析的层析柱规格：长度24cm，内径2cm。
凝胶过滤层析的柱填料：superdex200 increase 10/300GL(购自GEHealthcare公司，产品目录号为28-9909-44)。
进行如下操作步骤：上样0.5mL步骤(2)得到的浓缩液，用流速为0.5mL/min的PBS缓冲液(pH7.2、10mM)洗脱，收集目标峰对应的过柱后溶液，即为纯化后蛋白液。
待纯化蛋白液为步骤1制备的含SARS-CoV-2RBD蛋白的上清液时，凝胶过滤层析如图1，目标峰对应的保留体积为18.192mL，得到的纯化后蛋白液命名为SARS-CoV-2RBD蛋白溶液。
待纯化蛋白液为步骤2制备的含SARS-CoV-2S蛋白的上清液时，凝胶过滤层析如图2，目标峰对应的保留体积为8.633mL，得到的纯化后蛋白液命名为SARS-CoV-2S蛋白溶液。
待纯化蛋白液为步骤3制备的含SARS-CoV-1S蛋白的上清液时，凝胶过滤层析如图3，目标峰对应的保留体积为11.627mL，得到的纯化后蛋白液命名为SARS-CoV-1S蛋白溶液。
后续步骤以及后续实施例中用到的相关蛋白均是由纯化后蛋白液提供的。
二、免疫后羊驼纳米抗体展示文库筛选
1、羊驼的免疫和纳米抗体展示文库的建立
羊驼免疫过程依次如下：
第一次免疫：免疫SARS-CoV-2RBD蛋白，蛋白免疫剂量为200μg/只(具体是将蛋白溶液稀释至1mL，然后与1mL完全弗氏佐剂混匀后使用)；
第二次免疫：免疫SARS-CoV-2RBD蛋白，蛋白免疫剂量为200μg/只(具体是将蛋白溶液稀释至1mL，然后与1mL不完全弗氏佐剂混匀后使用)；
第三次免疫：免疫SARS-CoV-2RBD蛋白，蛋白免疫剂量为200μg/只(具体是将蛋白溶液稀释至1mL，然后与1mL不完全弗氏佐剂混匀后使用)；
第四次免疫：免疫腺病毒疫苗AdC68-19S，免疫剂量为1011vp/只；
第五次免疫：免疫SARS-CoV-2S蛋白，蛋白免疫剂量为200μg/只(具体是将蛋白溶液稀释至1mL，然后与1mL不完全弗氏佐剂混匀后使用)；
第六次免疫：免疫SARS-CoV-2S蛋白，蛋白免疫剂量为200μg/只(具体是将蛋白溶液稀释至1mL，然后与1mL不完全弗氏佐剂混匀后使用)。
六次免疫均采用颈部皮下免疫的方式。
第六次免疫7天后，颈部静脉采血50mL，送至成都阿帕克生物科技有限公司进行纳米抗体展示酵母文库的构建，得到多样性为108的酵母文库。
2、第一轮磁珠分选
(1)109个酵母细胞接种至100mL SDCAA培养基，30℃、250rpm培养至OD600＝7左右。
(2)完成步骤(1)后，4000rpm离心5分钟，弃上清，将沉淀重悬于SGCAA培养基(初始OD600＝0.5)，20℃、250rpm培养至OD600＝4左右。
(3)完成步骤(2)后，取109个细胞，用缓冲液(含1％FBS的PBS缓冲液，下同)洗涤，然后重悬于2mL缓冲液，加入钓饵蛋白(钓饵蛋白为SARS-CoV-2S蛋白或SARS-CoV-1S蛋白)并使其在体系中的浓度为100nM，室温孵育30分钟，冰上孵育10分钟，然后3500rpm离心5分钟，收集沉淀，用缓冲液洗涤。
(4)将步骤(3)获得的沉淀重悬于3mL缓冲液，加入200μL StreptavidinMicroBeads(Miltenyi)，冰上孵育10分钟(每2分钟翻转混匀一次)，然后加入5mL缓冲液混匀后离心，将沉淀重悬于10mL缓冲液，过70μm滤网并收集滤液(过滤是为了去除细胞团)，即为酵母磁珠悬液。
(5)LS柱(LS Columns，Miltenyi Biotec，130-042-401)置于磁力架上，用3mL冷缓冲液冲洗，加入8.5mL步骤(4)得到的酵母磁珠悬液，流尽后取下LS柱立即放回，加入1mL缓冲液冲洗，然后加入剩余的酵母磁珠悬液，流尽后用3mL缓冲液冲洗3遍，然后从磁力架取下LS柱，加入6mL缓冲液冲洗入15mL离心管。
(6)取步骤(5)得到的离心管，3500rpm离心5分钟，收集沉淀(此处收集的沉淀为酵母磁珠；在后续培养中未去除磁珠，但体系中的磁珠将随着细胞扩繁和传代被无限稀释)，重悬于1mL SDCAA培养基并培养24小时，然后离心收集沉淀，重悬于SGCAA培养基培养36小时。
3、第二轮流式分选
(1)完成步骤2后，离心收集细胞沉淀，用缓冲液洗涤，然后将108个细胞重悬于500μL缓冲液，加入钓饵蛋白(钓饵蛋白为SARS-CoV-2S蛋白或SARS-CoV-1S蛋白)并使其在体系中的浓度为100nM，冰上孵育30分钟，然后3500rpm离心5分钟，收集细胞沉淀并用缓冲液洗涤。
(2)完成步骤(1)后，将细胞重悬于500μL缓冲液，加入2.5μL抗HA-AF488抗体(CellSignaling)和2.5μL Streptavidin-PE抗体(eBioscience)，冰上孵育30分钟，用1mL缓冲液洗涤3遍后重悬于4mL缓冲液，过70μm滤网并收集滤液，即为酵母细胞悬液。
(3)将步骤(2)得到的酵母细胞悬液上机ArialII流式分选仪，分选FITC阳性且PE阳性的酵母至含有2mL SDCAA培养基的流式收集管中。
(4)完成步骤(3)后，从收集管中取2×103个酵母稀释在200μL SDCAA培养基中，涂布SDCAA平板，30℃培养过夜；剩余阳性细胞冻存。
4、阳性酵母单克隆的鉴定
(1)完成步骤3后，挑取SDCAA平板上的酵母单克隆至96孔深孔板中，每孔加入400μL SDCAA培养基，30℃、250rpm培养过夜。
(2)完成步骤(1)后，取样50μL，4000rpm离心5分钟，弃上清，沉淀重悬于400μLSGCAA培养基，20℃、250rpm培养36小时。
(3)完成步骤(2)后，取样100μL，离心弃除上清，沉淀用缓冲液洗涤，然后重悬于200μL缓冲液，加入钓饵蛋白(钓饵蛋白为SARS-CoV-2S蛋白或SARS-CoV-1S蛋白)并使其在体系中的浓度为50nM，冰上孵育30分钟，然后3500rpm离心5分钟，收集细胞沉淀，用缓冲液洗涤。
(4)将步骤(3)获得的细胞沉淀重悬于200μL缓冲液，加入1μL抗HA-AF488抗体和1μL Streptavidin-PE抗体，冰上孵育30分钟，离心收集细胞沉淀，用缓冲液洗涤后重悬于200μL缓冲液。
(5)将步骤(4)得到的细胞悬液，上机Fortessa流式分析仪，检测阳性酵母比例，选择阳性酵母比例为20％以上的双阳性酵母单克隆。
(6)提取双阳性酵母单克隆的酵母质粒，进行测序，获得纳米抗体基因序列。
共获得4种纳米抗体，依次命名为TH-34纳米抗体、TH-41纳米抗体、TH-44纳米抗体、TH-40纳米抗体。
TH-34纳米抗体的氨基酸序列如序列表的序列8所示，其编码基因如序列表的序列9所示。所述TH-41纳米抗体的氨基酸序列如序列表的序列10所示，其编码基因如序列表的序列11所示。所述TH-44纳米抗体的氨基酸序列如序列表的序列12所示，其编码基因如序列表的序列13所示。所述TH-40纳米抗体的氨基酸序列如序列表的序列14所示，其编码基因如序列表的序列15所示。
实施例2、纳米抗体-Fc融合蛋白的制备
一、重组表达载体的构建
1、在pMD18-T载体的EcoRV酶切位点插入序列表的序列16所示的双链DNA分子，得到人源Fc载体。序列表的序列16中，第51-354位核苷酸组成CMV Enhancer，第355-558位核苷酸组成CMV Promoter，第947-952核苷酸组成NheI酶切识别序列，第954-961位核苷酸组成NotI酶切识别序列，第977-1672位核苷酸编码人源Fc(人源Fc的氨基酸序列如序列表的序列32所示)，第1673-1675位核苷酸为终止密码子，第1748-1869位核苷酸组成SV40Poly(A)。
2、将人源Fc载体的NheI和NotI酶切识别序列之间的小片段(含NheI酶切识别序列和NotI酶切识别序列，即GCTAGCCGCGGCCGC)替换为序列表的序列9所示的双链DNA分子，得到重组质粒TH-34-Fc。重组质粒TH-34-Fc表达如下融合蛋白，自N端至C端依次由信号肽、序列8所示蛋白区段(TH-34纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列8所示蛋白区段(TH-34纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。
3、将人源Fc载体的NheI和NotI酶切识别序列之间的小片段(含NheI酶切识别序列和NotI酶切识别序列，即GCTAGCCGCGGCCGC)替换为序列表的序列11所示的双链DNA分子，得到重组质粒TH-41-Fc。重组质粒TH-41-Fc表达如下融合蛋白，自N端至C端依次由信号肽、序列10所示蛋白区段(TH-41纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列10所示蛋白区段(TH-41纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)。
4、将人源Fc载体的NheI和NotI酶切识别序列之间的小片段(含NheI酶切识别序列和NotI酶切识别序列，即GCTAGCCGCGGCCGC)替换为序列表的序列13所示的双链DNA分子，得到重组质粒TH-44-Fc。重组质粒TH-44-Fc表达如下融合蛋白，自N端至C端依次由信号肽、序列12所示蛋白区段(TH-44纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列12所示蛋白区段(TH-44纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)。
5、将人源Fc载体的NheI和NotI酶切识别序列之间的小片段(含NheI酶切识别序列和NotI酶切识别序列，即GCTAGCCGCGGCCGC)替换为序列表的序列15所示的双链DNA分子，得到重组质粒TH-40-Fc。重组质粒TH-40-Fc表达如下融合蛋白，自N端至C端依次由信号肽、序列14所示蛋白区段(TH-40纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列14所示蛋白区段(TH-40纳米抗体)、连接肽(GGGGS)和序列32所示蛋白区段(人源Fc)组成。
二、制备纳米抗体-Fc融合蛋白
1、将步骤一制备的重组质粒转染293F细胞，然后采用SMM 293-TII培养基培养72h，然后4℃、4000rpm离心30min，收集上清液。
2、亲和层析
亲和层析的层析柱规格：长度3cm，内径1cm；
亲和层析的柱填料：protein A beads(Thermo，产品目录号10006D)；
依次进行如下操作步骤：①将300mL步骤1得到的上清液上样于亲和层析柱，4℃孵育16小时；②用60mL PBS缓冲液(pH7.2、10mM)洗涤柱子；③用30mL洗脱缓冲液洗脱目的蛋白，收集过柱后溶液。
洗脱缓冲液：取甘氨酸7.5g，溶于水并用水定容至500mL，用盐酸调pH至3.0。
3、取步骤2得到的过柱后溶液，用超滤浓缩管浓缩并将体系置换为PBS缓冲液(pH7.2、10mM)，得到1mL抗体溶液(抗体浓度约为2mg/mL)。
重组质粒TH-34-Fc进行步骤二，得到的抗体溶液命名为TH-34-Fc抗体溶液。
重组质粒TH-41-Fc进行步骤二，得到的抗体溶液命名为TH-41-Fc抗体溶液。
重组质粒TH-44-Fc进行步骤二，得到的抗体溶液命名为TH-44-Fc抗体溶液。
重组质粒TH-40-Fc进行步骤二，得到的抗体溶液命名为TH-40-Fc抗体溶液。
实施例3、纳米抗体-Fc融合蛋白对SARS-CoV-2以及其他沙贝病毒的中和活性的检测
新型冠状病毒膜蛋白来源如下：
7种新型冠状病毒毒株的膜蛋白来源如下：
野生型新型冠状病毒(Genbank：MN908947.3)；
新型冠状病毒Alpha株(GISAID：EPI_ISL_601443)，包含9个突变：69-70del、144del、N501Y、A570D、D614G、P681H、T716I、S982A、D1118H；B.1.1.7；
新型冠状病毒Beta株(GISAID：EPI_ISL_700450)，包含10个突变：L18F、D80A、D215G、242-244del、S305T、K417N、E484K、N510Y、D614G、A701V；B.1.351；
新型冠状病毒Gamma株(GISAID：EPI_ISL_792681)，包含12个突变：L18F、T20N、P26S、D138Y、R190S、K417T、E484K、N501Y、D614G、H655Y、T1027I、V1176F；P.1。
新型冠状病毒Delta株(GISAID：EPI_ISL_1534938)，包含个10个突变：T19R、G142D、156-157del、R158G、A222V、L452R、T478K、D614G、P681R、D950N；B.1.617.2；
新型冠状病毒Omicron BA.1株(GISAID：EPI_ISL_6752027)，包含个34个突变：A67V、69-70del、T95I、G142D、143-145del、211del、L212I、ins214EPE、G339D、S371L、S373P、S375F、K417N、N440K、G446S、S477N、T478K、E484A、Q493R、G496S、Q498R、N501Y、Y505H、T547K、D614G、H655Y、N679K、P681H、N764K、D796Y、N856K、Q954H、N969K、L981F；BA.1；
新型冠状病毒Omicron BA.2株(GISAID：EPI_ISL_8515362)，包含个29个突变：T19I、24-26del、A27S、G142D、V213G、G339D、S371F、S373P、S375F、T376A、D405N、R408S、K417N、N440K、S477N、T478K、E484A、Q493R、Q498R、N501Y、Y505H、D614G、H655Y、N679K、P681H、N764K、D796Y、N969K、Q954H；BA.2；
一、假病毒的制备
表达病毒膜蛋白的质粒和骨架质粒pNL4-3R-E-luciferase共转染293T细胞，孵育后能够得到具有感染性但没有复制能力的假病毒，其感染性同活病毒相似。骨架质粒pNL4-3R-E-luciferase，即含有Luciferase的骨架质粒pNL4-3R-E(即文献中的vector with theluciferase gene containing backbone pNL4-3R-E)：Wang Q,Liu L,Ren W,Gettie A,Wang H,Liang Q,Shi X,Montefiori DC,Zhou T,Zhang L.Cell Rep.2019。
将病毒(病毒指的是上述7种新型冠状病毒毒株中的任意一种或者5种沙贝病毒毒株的任意一种)的膜蛋白的编码基因插入pcDNA3.1(+)载体的BamH II和EcoR I酶切位点之间，得到表达病毒膜蛋白的质粒。将表达膜蛋白的质粒和骨架质粒pNL4-3R-E-luciferase共转染293T细胞，37℃静置孵育(采用含10％胎牛血清的DMEM培养基)，转染60小时后收集细胞培养上清，即为含有相应假病毒的病毒液。
野生型新型冠状病毒中，膜蛋白如序列表的序列17所示。新型冠状病毒膜蛋白的编码基因如序列表的序列18所示时(编码序列表的序列17所示的蛋白质)，进行上述步骤，得到野生型新型冠状病毒假病毒。在序列表的序列18的基础上增加各种突变(新型冠状病毒Alpha株的相应突变见表1，新型冠状病毒Beta株的相应突变见表2，新型冠状病毒Gamma株的相应突变见表3，新型冠状病毒Delta株的相应突变见表4)，然后作为病毒膜蛋白的编码基因，进行上述步骤，分别得到新型冠状病毒Alpha株假病毒、新型冠状病毒Beta株假病毒、新型冠状病毒Gamma株假病毒、新型冠状病毒Delta株假病毒。新型冠状病毒OmicronBA.1株膜蛋白的编码基因如序列表的序列19所示，进行上述步骤，得到新型冠状病毒Omicron BA.1株假病毒。新型冠状病毒Omicron BA.2株膜蛋白的编码基因如序列表的序列20所示，进行上述步骤，得到新型冠状病毒Omicron BA.2株假病毒。
表1
蛋白质突变 DNA突变(相对应于序列18的位置)
69-70del(缺失两个氨基酸残基“HV”) 缺失205-210位的“CACGTG”
144del(缺失一个氨基酸残基“Y”) 缺失430-432位的“TAT”
N501Y(一个氨基酸残基突变) 1501位的“A”突变为“T”
A570D(一个氨基酸残基突变) 1709位的“C”突变为“A”
D614G(一个氨基酸残基突变) 1841位的“A”突变为“G”
P681H(一个氨基酸残基突变) 2042位的“C”突变为“A”
T716I(一个氨基酸残基突变) 2147位的“C”突变为“T”
S982A(一个氨基酸残基突变) 2944-2945位的“AG”突变为“GC”
D1118H(一个氨基酸残基突变) 3352位的“G”突变为“C”
表2
蛋白质突变 DNA突变(相对应于序列18的位置)
L18F(一个氨基酸残基突变) 52位的“C”突变为“T”，54位的“G”突变为“C”
D80A(一个氨基酸残基突变) 239位的“A”突变为“C”
D215G(一个氨基酸残基突变) 644位的“A”突变为“G”
242-244del(缺失三个氨基酸残基) 缺失724-732位的“CTGGCCCTG”
S305T(一个氨基酸残基突变) 914位的“G”突变为“C”
K417N(一个氨基酸残基突变) 1251位的“G”突变为“C”
E484K(一个氨基酸残基突变) 1450位的“G”突变为“A”
N501Y(一个氨基酸残基突变) 1501位的“A”突变为“T”，1503位的“T”突变为“C”
D614G(一个氨基酸残基突变) 1841位的“A”突变为“G”
A701V(一个氨基酸残基突变) 2102-2103位的“CC”突变为“TG”
表3
表4
蛋白质突变 DNA突变(相对应于序列18的位置)
T19R(一个氨基酸残基突变) 55位的“A”突变为“C”，56位的“C”突变为“G”
G142D(一个氨基酸残基突变) 425位的“G”突变为“A”
156-157del(缺失两个氨基酸残基) 缺失467-472位的“AGTTCC”
R158G(一个氨基酸残基突变) 474位的“C”突变为“T”
A222V(一个氨基酸残基突变) 665位的“C”突变为“T”
L452R(一个氨基酸残基突变) 1355位的“T”突变为“G”
T478K(一个氨基酸残基突变) 1433位的“C”突变为“A”
D614G(一个氨基酸残基突变) 1841位的“A”突变为“G”
P681R(一个氨基酸残基突变) 2041-2043位的“CCT”突变为“AGA”
D950N(一个氨基酸残基突变) 2848位的“G”突变为“A”，2850位的“C”突变为“T”
SARS-CoV-1的膜蛋白如序列表的序列21所示，膜蛋白的编码基因如序列表的序列22所示，进行上述步骤，得到SARS-CoV-1假病毒。Pangolin CoV GD的膜蛋白如序列表的序列23所示，膜蛋白的编码基因如序列表的序列24所示，进行上述步骤，得到Pangolin CoVGD假病毒。Pangolin CoV GX的膜蛋白如序列表的序列25所示，膜蛋白的编码基因如序列表的序列26所示，进行上述步骤，得到Pangolin CoV GX假病毒。Bat CoV WIV16的膜蛋白如序列表的序列27所示，膜蛋白的编码基因如序列表的序列28所示，进行上述步骤，得到BatCoV WIV16假病毒。Bat CoV RaTG13的膜蛋白如序列表的序列29所示，膜蛋白的编码基因如序列表的序列30所示，进行上述步骤，得到Bat CoV RaTG13假病毒。
二、单克隆抗体的中和活性检测
供试抗体为：TH-34-Fc(实施例2中制备的TH-34-Fc抗体溶液)、TH-41-Fc(实施例2中制备的TH-41-Fc抗体溶液)、TH-44-Fc(实施例2中制备的TH-44-Fc抗体溶液)或TH-40-Fc(实施例2中制备的TH-40-Fc抗体溶液)。
1、采用含10％FBS的DMEM培养基将供试抗体进行稀释，得到稀释液。
2、将100μl步骤1得到的稀释液与50μl步骤一制备的病毒液(病毒含量为100TCID50)混合，37℃静置孵育1小时。设置用100μl含10％FBS的DMEM培养基代替100μl稀释液的空白对照。
3、完成步骤2后，加入50μl hACE2-hela细胞悬液(约含2×104个细胞)，37℃静置孵育48小时。
4、完成步骤3后，加入100μl PBS缓冲液和50μl细胞裂解液(Bright-GloTMLuciferase Assay System，Promega，E2650)，静置2min，然后用化学发光仪检测荧光素酶活性。
每种处理设置3个复孔，结果取平均值。
中和活性＝(空白对照组的荧光强度-加入稀释液的实验组的荧光强度)/空白对照组的荧光强度×100％。
中和活性为50％时对应的抗体浓度即为IC50值。
TH-34-Fc、TH-41-Fc、TH-44-Fc和TH-40-Fc四个抗体对野生型新型冠状病毒以及自然变异株均具有强中和能力，IC50值结果见表5。表5中，数据单位为μg/ml。表5中，N.D.代表TH-40-Fc对Omicron BA.2无中和活性。
TH-34-Fc、TH-41-Fc、TH-44-Fc和TH-40-Fc四个抗体对其他5个沙贝病毒均具有强中和能力，IC50值结果见表6。表6中，数据单位为μg/ml。
表5
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表6
纳米抗体 SARS-CoV-1 Pangolin CoV GD Pangolin CoV GX Bat CoV WIV16 Bat CoV RaTG13
TH-34-Fc 0.016 0.034 0.501 0.050 0.037
TH-41-Fc 0.058 0.015 0.313 0.037 0.031
TH-44-Fc 0.040 0.041 0.347 0.098 0.042
TH-40-Fc 0.080 0.012 0.015 0.008 0.010
实施例4、纳米抗体的制备
一、重组表达载体的构建
1、在pVRC8400载体的NotI和BamHI酶切位点插入序列表的序列31所示的双链DNA分子，得到pVRC8400-His tag载体。序列表的序列31中，第1-57位核苷酸编码信号肽，第58-65位核苷酸组成NotI酶切识别序列，第81-86位核苷酸组成NheI酶切识别序列，第87-104位核苷酸编码His6标签，第105-107位核苷酸为终止密码子。
2、将pVRC8400-His tag载体中NotI和BamHI酶切识别序列中的小片段(含NotI酶切识别序列和BamHI酶切识别序列，即GCGGCCGCGGAGGTGGAGGTAGTGCTAGC)替换为序列表的序列9所示的双链DNA分子，得到重组质粒TH-34。重组质粒TH-34表达如下融合蛋白，自N端至C端依次由信号肽、序列8所示蛋白区段(TH-34纳米抗体)和His6标签组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列8所示蛋白区段(TH-34纳米抗体)和His6标签组成。
3、将pVRC8400-His tag载体中NotI和BamHI酶切识别序列中的小片段(含NotI酶切识别序列和BamHI酶切识别序列，即GCGGCCGCGGAGGTGGAGGTAGTGCTAGC)序列表的序列11所示的双链DNA分子，得到重组质粒TH-41。重组质粒TH-41表达如下融合蛋白，自N端至C端依次由信号肽、序列10所示蛋白区段(TH-41纳米抗体)和His6标签组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列10所示蛋白区段(TH-41纳米抗体)和His6标签组成。
4、将pVRC8400-His tag载体中NotI和BamHI酶切识别序列中的小片段(含NotI酶切识别序列和BamHI酶切识别序列，即GCGGCCGCGGAGGTGGAGGTAGTGCTAGC)序列表的序列13所示的双链DNA分子，得到重组质粒TH-44。重组质粒TH-44表达如下融合蛋白，自N端至C端依次由信号肽、序列12所示蛋白区段(TH-44纳米抗体)和His6标签组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列12所示蛋白区段(TH-44纳米抗体)和His6标签组成。
5、将pVRC8400-His tag载体中NotI和BamHI酶切识别序列中的小片段(含NotI酶切识别序列和BamHI酶切识别序列，即GCGGCCGCGGAGGTGGAGGTAGTGCTAGC)序列表的序列15所示的双链DNA分子，得到重组质粒TH-40。重组质粒TH-40表达如下融合蛋白，自N端至C端依次由信号肽、序列14所示蛋白区段(TH-40纳米抗体)和His6标签组成。在细胞中信号肽被切除，剩余如下活性蛋白，自N端至C端依次由序列14所示蛋白区段(TH-40纳米抗体)和His6标签组成。
二、制备纳米抗体蛋白
重组质粒分别为：重组质粒TH-34、重组质粒TH-41、重组质粒TH-44或重组质粒TH-40。
1、将步骤一制备的重组质粒转染293F细胞，然后采用SMM 293-TII培养基培养72h，然后4℃、4000rpm离心30min，收集上清液。
2、亲和层析
亲和层析的层析柱规格：长度3cm，内径1cm；
亲和层析的柱填料：镍柱beads(购自Qiagen公司，产品目录号为30230)。
依次进行如下操作步骤：①将200ml步骤1得到的上清液上样于亲和层析柱，4℃孵育3小时；②用100mL含20mM咪唑的HEPEs buffer(pH7.2、1M)洗涤柱子；③用30mL含500mM咪唑的HEPEs buffer(pH7.2、1M)洗脱目的蛋白，收集过柱后溶液。
3、取步骤2得到的过柱后溶液，用超滤浓缩管浓缩并将体系置换为PBS缓冲液(pH7.2、10mM)，得到1mL抗体溶液(抗体浓度约为4mg/mL)。
重组质粒TH-34进行步骤二，得到的抗体溶液命名为TH-34抗体溶液。
重组质粒TH-41进行步骤二，得到的抗体溶液命名为TH-41抗体溶液。
重组质粒TH-44进行步骤二，得到的抗体溶液命名为TH-44抗体溶液。
重组质粒TH-40进行步骤二，得到的抗体溶液命名为TH-40抗体溶液。
实施例5、纳米抗体对SARS-CoV-2以及SARS-CoV-1的中和活性的检测
供试假病毒分别为：实施例3制备的野生型新型冠状病毒假病毒或SARS-CoV-1假病毒。
供试抗体分别为：TH-34(实施例4中制备的TH-34抗体溶液)、TH-41(实施例4中制备的TH-41抗体溶液)、TH-44(实施例4中制备的TH-44抗体溶液)或TH-40(实施例4中制备的TH-40抗体溶液)。
检测供试抗体的中和活性，方法同实施例3的步骤二。
结果见图4和图5。
TH-34、TH-41、TH-44和TH-40四个抗体对野生型新型冠状病毒以及SARS-CoV-1均具有强中和能力，针对野生型新型冠状病毒的IC50值依次为0.027、0.021、0.020和0.053μg/ml，针对SARS-CoV-1病毒的IC50值依次为0.371、0.125、0.187和0.113μg/ml。
以上对本发明进行了详述。对于本领域技术人员来说，在不脱离本发明的宗旨和范围，以及无需进行不必要的实验情况下，可在等同参数、浓度和条件下，在较宽范围内实施本发明。虽然本发明给出了特殊的实施例，应该理解为，可以对本发明作进一步的改进。总之，按本发明的原理，本申请欲包括任何变更、用途或对本发明的改进，包括脱离了本申请中已公开范围，而用本领域已知的常规技术进行的改变。按以下附带的权利要求的范围，可以进行一些基本特征的应用。
序列表
<110> 清华大学
<120> 广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用
<130> CGGNQYX226060
<160> 32
<170> SIPOSequenceListing 1.0
<210> 1
<211> 278
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 1
Met Leu Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly Ala Val Phe
1 5 10 15
Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg Gly Ala Arg
20 25 30
Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr
35 40 45
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser
50 55 60
Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr
65 70 75 80
Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly
85 90 95
Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala
100 105 110
Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly
115 120 125
Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe
130 135 140
Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val
145 150 155 160
Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu
165 170 175
Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser
180 185 190
Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln
195 200 205
Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg
210 215 220
Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys
225 230 235 240
Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe
245 250 255
Trp Ser His Pro Gln Phe Glu Lys Asp Tyr Lys Asp Asp Asp Asp Lys
260 265 270
His His His His His His
275
<210> 2
<211> 837
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
atgctgcgcg gactgtgctg cgtgctgcta ctgtgcggcg ccgtgttcgt gagccccagc 60
caggagatcc acgcccgatt caggagagga gccagaggac gcgtgcagcc caccgagagc 120
atcgtgcgct tccccaacat caccaacctg tgccccttcg gcgaggtgtt caacgccacc 180
cgcttcgcca gcgtgtacgc ctggaaccgc aagcgcatca gcaactgcgt ggccgactac 240
agcgtgctgt acaacagcgc cagcttcagc accttcaagt gctacggcgt gagccccacc 300
aagctgaacg acctgtgctt caccaacgtg tacgccgaca gcttcgtgat ccgcggcgac 360
gaggtgcgcc agatcgcccc cggccagacc ggcaagatcg ccgactacaa ctacaagctg 420
cccgacgact tcaccggctg cgtgatcgcc tggaacagca acaacctgga cagcaaggtg 480
ggcggcaact acaactacct gtaccgcctg ttccgcaaga gcaacctgaa gcccttcgag 540
cgcgacatca gcaccgagat ctaccaggcc ggcagcaccc cctgcaacgg cgtggagggc 600
ttcaactgct acttccccct gcagagctac ggcttccagc ccaccaacgg cgtgggctac 660
cagccctacc gcgtggtggt gctgagcttc gagctgctgc acgcccccgc caccgtgtgc 720
ggccccaaga agagcaccaa cctggtgaag aacaagtgcg tgaacttctg gagccacccc 780
cagttcgaga aggactacaa ggacgacgac gacaagcacc accaccacca ccactga 837
<210> 3
<211> 1269
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 3
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Leu Glu Val Leu Phe
1205 1210 1215
Gln Gly Pro Gly Gly Gly Ser Gly Gly Gly Ser Gly Tyr Ile Pro Glu
1220 1225 1230
Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val
1235 1240 1245
Leu Leu Ser Thr Phe Leu Gly Trp Ser His Pro Gln Phe Glu Lys His
1250 1255 1260
His His His His His
1265
<210> 4
<211> 3810
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
atgttcgtgt tcctggtgct gctgcctctg gtgagcagcc agtgcgtgaa tctgaccacc 60
agaacccagc tgcctcctgc ctacaccaat agcttcacca gaggagttta ttatcccgat 120
aaggtgttca gaagtagtgt attacatagt acccaggacc tgttcctacc tttcttcagt 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcaccaa gagattcgac 240
aatcctgtgc tgcctttcaa tgacggcgtg tacttcgcca gcaccgagaa gagcaatatc 300
atcagaggct ggatcttcgg caccaccttg gattccaaga ctcagagcct gctgattgta 360
aacaacgcta caaatgtggt gatcaaggtg tgcgagttcc agttctgcaa tgaccctttc 420
ctgggtgttt attatcataa gaacaacaag agctggatgg agagcgagtt ccgcgtatat 480
tcgtcggcta ataattgcac cttcgagtac gtgagccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaatctgaga gagttcgtgt tcaagaatat cgacggctac 600
ttcaagatct acagcaagca cacacccatt aatctggtga gagacctgcc tcagggcttc 660
agcgccctgg agcctctggt ggacctgcct atcggcatca atatcaccag attccagacc 720
ctgctggccc tgcacagatc atatcttaca ccaggcgatt cgtcaagcgg ttggaccgct 780
ggagctgcgg catattacgt gggctacctg cagcctagaa ccttcctgct gaagtacaat 840
gagaatggta cgataaccga cgcagttgat tgtgccctgg accctctgag cgagaccaag 900
tgcaccctga agagcttcac cgtggagaag ggcatctacc agaccagcaa tttcagagtg 960
cagcctaccg agagcatcgt gagattccct aatatcacca atctgtgccc tttcggcgag 1020
gtgttcaatg ccaccagatt cgccagcgtg tacgcatgga accgcaagcg gataagcaat 1080
tgcgtggccg actacagcgt gctgtacaat agcgccagct tcagcacctt caaatgttat 1140
ggtgtttcgc caacaaagct gaatgacctg tgcttcacca atgtgtacgc cgacagcttc 1200
gtgatcagag gcgacgaggt gagacagatc gcgccagggc agaccggcaa gatcgccgac 1260
tacaattaca agctgcctga cgacttcacc ggctgcgtga tcgcgtggaa ctctaacaat 1320
ctagattcga aagttggagg caattacaat tacctgtaca gactgttcag aaagagcaat 1380
ctgaagcctt tcgagagaga catcagcacc gagatctacc aggccggcag cacaccgtgt 1440
aatggcgtgg agggcttcaa ttgctacttc cctctgcaga gctacggctt ccagcctacc 1500
aatggcgtgg gctaccagcc ttacagagtg gtggtgctga gcttcgagct gctgcacgct 1560
cccgctaccg tgtgcggccc taagaagagc accaatctgg tgaagaataa gtgcgtgaat 1620
ttcaatttca atggtctaac tggaacgggc gtgctgaccg agagcaataa gaagtttctt 1680
ccctttcaac aattcggcag agacatcgcc gacaccacag atgctgtaag agaccctcag 1740
accctggaga tcctggacat cactccgtgt agcttcggcg gcgtgagcgt gatcacaccg 1800
ggtaccaata ccagcaatca ggtggccgtg ctgtaccagg acgtgaattg caccgaggtg 1860
cctgtggcca tccacgccga ccagctgact cccacttgga gggtatattc cacgggaagc 1920
aatgtgttcc agaccagagc cggctgcctg atcggcgccg agcacgtgaa taatagctac 1980
gagtgcgaca tccctatcgg cgccggcatc tgcgccagct accagaccca gaccaatagc 2040
cctggaagcg caagcagcgt ggccagccag agcatcatcg cctacaccat gagcctgggc 2100
gccgagaata gcgtggccta cagcaataat agcatcgcca tccctaccaa tttcaccatc 2160
agcgtgacca ccgaaatatt accagtctcc atgaccaaga ccagcgtgga ctgcaccatg 2220
tacatctgcg gcgacagcac cgagtgcagc aatctgctgc tgcagtacgg cagcttctgc 2280
acccagctga atagagccct gaccggcatc gccgtggagc aggacaagaa tacccaggag 2340
gtgttcgccc aggtgaagca gatctacaag actccgccga tcaaggactt cggcggcttc 2400
aatttcagcc aaatactccc agatccaagc aagcctagca agaggagctt catcgaggac 2460
ctgctgttca ataaggtgac cctggccgac gccggcttca tcaagcagta cggcgactgc 2520
ctaggtgata ttgcggcaag agacctgatc tgcgcccaga agtttaacgg tttgacagta 2580
ctacctcctc tgctgaccga cgagatgata gcacaatata cgtcggcatt gctcgctggc 2640
acgatcacat cgggctggac tttcggcgcc ggagcagcgt tgcaaatccc tttcgccatg 2700
cagatggcct acagattcaa tggcatcggc gtgacccaga atgtgctgta cgagaatcag 2760
aagctgatcg ccaatcagtt caatagcgcc atcggcaaga tccaggacag cctgagcagc 2820
accgccagcg ccctgggcaa gctgcaggac gtggtgaatc agaatgccca ggccctgaat 2880
accctggtga agcagctgag cagcaatttc ggcgccatca gtagtgtact caacgatatc 2940
ctgagcagac tggacccgcc ggaggccgag gtgcaaattg atcgtcttat tactggcaga 3000
ctgcagagcc tgcagaccta cgtgacccag cagctgatca gagccgccga gatcagagcc 3060
agcgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagag caagagagtg 3120
gacttctgcg gcaagggcta ccacctgatg agcttccctc agagcgctcc acatggcgtg 3180
gtgttcctgc acgtgaccta cgtgcctgcc caggagaaga atttcaccac cgcacccgca 3240
atctgccacg acggcaaggc ccacttccct agagagggcg tgttcgtgag caatggcacc 3300
cactggttcg tgacccagag aaatttctac gagcctcaga tcatcaccac cgacaatacc 3360
ttcgtgagcg gcaattgcga cgtggtgatc gggatagtca ataatactgt ctacgaccct 3420
ctgcagcctg agctggacag cttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccctgacg tggacctcgg tgatatttcg ggaatcaatg ccagcgtggt gaatatccag 3540
aaggaaattg atcggctcaa cgaagtggcc aagaatctga atgagagcct gatcgacctg 3600
caggagctgg gcaagtacga gcagtacatc aagctggaag ttctgttcca ggggcccgga 3660
ggaggaagtg gaggaggaag tggctatatt ccggaagcgc cgcgcgatgg ccaggcgtat 3720
gtgcgcaaag atggcgaatg ggtgctgctg agcacctttc tgggctggtc ccaccctcag 3780
ttcgagaagc accaccacca ccaccactga 3810
<210> 5
<211> 1211
<212> PRT
<213> SARS-CoV-2
<400> 5
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Lys Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys
1205 1210
<210> 6
<211> 1253
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 6
Met Phe Ile Phe Leu Leu Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu
1 5 10 15
Asp Arg Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Thr Gln
20 25 30
His Thr Ser Ser Met Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg
35 40 45
Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser
50 55 60
Asn Val Thr Gly Phe His Thr Ile Asn His Thr Phe Gly Asn Pro Val
65 70 75 80
Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser Asn
85 90 95
Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln
100 105 110
Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys
115 120 125
Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Met
130 135 140
Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr
145 150 155 160
Phe Glu Tyr Ile Ser Asp Ala Phe Ser Leu Asp Val Ser Glu Lys Ser
165 170 175
Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly
180 185 190
Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp
195 200 205
Leu Pro Ser Gly Phe Asn Thr Leu Lys Pro Ile Phe Lys Leu Pro Leu
210 215 220
Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Ser Pro
225 230 235 240
Ala Gln Asp Ile Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr
245 250 255
Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile
260 265 270
Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys
275 280 285
Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn
290 295 300
Phe Arg Val Val Pro Ser Gly Asp Val Val Arg Phe Pro Asn Ile Thr
305 310 315 320
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser
325 330 335
Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr
340 345 350
Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly
355 360 365
Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala
370 375 380
Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly
385 390 395 400
Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe
405 410 415
Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser
420 425 430
Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu
435 440 445
Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly
450 455 460
Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp
465 470 475 480
Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val
485 490 495
Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly
500 505 510
Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn
515 520 525
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg
530 535 540
Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr Asp
545 550 555 560
Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys
565 570 575
Ala Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Ala Ser Ser
580 585 590
Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Ser Thr
595 600 605
Ala Ile His Ala Asp Gln Leu Thr Pro Ala Trp Arg Ile Tyr Ser Thr
610 615 620
Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu
625 630 635 640
His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile
645 650 655
Cys Ala Ser Tyr His Thr Val Ser Leu Leu Arg Ser Thr Ser Gln Lys
660 665 670
Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala
675 680 685
Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile
690 695 700
Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys
705 710 715 720
Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu
725 730 735
Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile
740 745 750
Ala Ala Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys
755 760 765
Gln Met Tyr Lys Thr Pro Thr Leu Lys Tyr Phe Gly Gly Phe Asn Phe
770 775 780
Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile
785 790 795 800
Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met
805 810 815
Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile
820 825 830
Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr
835 840 845
Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala
850 855 860
Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe
865 870 875 880
Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn
885 890 895
Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala
900 905 910
Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly
915 920 925
Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu
930 935 940
Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn
945 950 955 960
Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp
965 970 975
Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln
980 985 990
Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala
995 1000 1005
Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe
1010 1015 1020
Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ala Ala Pro His
1025 1030 1035 1040
Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ser Gln Glu Arg Asn
1045 1050 1055
Phe Thr Thr Ala Pro Ala Ile Cys His Glu Gly Lys Ala Tyr Phe Pro
1060 1065 1070
Arg Glu Gly Val Phe Val Phe Asn Gly Thr Ser Trp Phe Ile Thr Gln
1075 1080 1085
Arg Asn Phe Phe Ser Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val
1090 1095 1100
Ser Gly Asn Cys Asp Val Val Ile Gly Ile Ile Asn Asn Thr Val Tyr
1105 1110 1115 1120
Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys
1125 1130 1135
Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser
1140 1145 1150
Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu
1155 1160 1165
Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu
1170 1175 1180
Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Leu Glu Val Leu Phe
1185 1190 1195 1200
Gln Gly Pro Gly Gly Gly Ser Gly Gly Gly Ser Gly Tyr Ile Pro Glu
1205 1210 1215
Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val
1220 1225 1230
Leu Leu Ser Thr Phe Leu Gly His His His His His His Trp Ser His
1235 1240 1245
Pro Gln Phe Glu Lys
1250
<210> 7
<211> 3762
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
atgttcatct tcctgctgtt cctgaccctg accagcggca gcgacctgga ccggtgcacc 60
accttcgacg acgtgcaggc ccccaactac acccagcaca ccagcagcat gcggggcgtg 120
tactaccccg acgagatctt ccggagcgac accctgtacc tgacccagga cctgttcctg 180
cccttctaca gcaacgtgac cggcttccac accatcaacc acaccttcgg caaccccgtg 240
atccccttca aggacggcat ctacttcgcc gccaccgaga agagcaacgt ggtgcggggc 300
tgggtgttcg gcagcaccat gaacaacaag agccagagcg tgatcatcat caacaacagc 360
accaacgtgg tgatccgggc ctgcaacttc gagctgtgcg acaacccctt cttcgccgtg 420
agcaagccca tgggcaccca gacccacacc atgatcttcg acaacgcctt caactgcacc 480
ttcgagtaca tcagcgacgc cttcagcctg gacgtgagcg agaagagcgg caacttcaag 540
cacctgcggg agttcgtgtt caagaacaag gacggcttcc tgtacgtgta caagggctac 600
cagcccatcg acgtggtgcg ggacctgccc agcggcttca acaccctgaa gcccatcttc 660
aagctgcccc tgggcatcaa catcaccaac ttccgggcca tcctgaccgc cttcagcccc 720
gcccaggaca tctggggcac cagcgccgcc gcctacttcg tgggctacct gaagcccacc 780
accttcatgc tgaagtacga cgagaacggc accatcaccg acgccgtgga ctgcagccag 840
aaccccctgg ccgagctgaa gtgcagtgtg aagagcttcg agatcgacaa gggcatctac 900
cagaccagca acttccgggt ggtgcccagc ggcgacgtgg tgcggttccc caacatcacc 960
aacctgtgcc ccttcggcga ggtgttcaac gccaccaagt tccccagcgt gtacgcctgg 1020
gagcggaaga agatcagcaa ctgcgtggcc gactacagcg tgctgtacaa cagcaccttc 1080
ttcagcacct tcaagtgcta cggcgtgagc gccaccaagc tgaacgacct gtgcttcagc 1140
aacgtgtacg ccgacagctt cgtggtgaag ggcgacgacg tgcggcagat cgcccccggc 1200
cagaccggcg tgatcgccga ctacaactac aagctgcccg acgacttcat gggctgcgtg 1260
ctggcctgga acacccggaa catcgacgcc accagcaccg gcaactacaa ctacaagtac 1320
cggtacctgc ggcacggcaa gctgcggccc ttcgagcggg acatcagcaa cgtgcccttc 1380
agccccgacg gcaagccctg cacccccccc gccctgaact gctactggcc cctgaacgac 1440
tacggcttct acaccactac cggcatcggc taccagccct accgggtggt ggtgctgagc 1500
ttcgagctgc tgaacgcccc cgccaccgtg tgcggcccca agctgagcac cgacctgatc 1560
aagaaccagt gcgtgaactt caacttcaac ggcctgaccg gcaccggcgt gctgaccccc 1620
agcagcaagc ggttccagcc cttccagcag ttcggccggg acgtgagcga cttcaccgac 1680
agcgtgcggg accccaagac cagcgagatc ctggacatca gcccctgcgc cttcggcggc 1740
gtgagcgtga tcacccccgg taccaacgcc agcagcgagg tggccgtgct gtaccaggac 1800
gtgaactgca ccgacgtgag caccgccatc cacgccgacc agctgacccc cgcctggcgg 1860
atctacagca ccggcaacaa cgtgttccag acccaggccg gctgcctgat cggcgccgag 1920
cacgtggaca ccagctacga gtgcgacatc cccatcggcg ccggcatctg cgccagctac 1980
cacaccgtga gcctgctgcg gagcaccagc cagaagagca tcgtggccta caccatgagc 2040
ctgggcgccg acagcagcat cgcctacagc aacaacacca tcgccatccc caccaacttc 2100
agcatcagca tcaccaccga ggtgatgccc gtgagcatgg ccaagaccag cgtggactgc 2160
aacatgtaca tctgcggcga cagcaccgag tgcgccaacc tgctgctgca gtacggcagc 2220
ttctgcaccc agctgaaccg ggccctgagc ggcatcgccg ccgagcagga ccggaacacc 2280
cgggaggtgt tcgcccaggt gaagcagatg tacaagaccc ccaccctgaa gtacttcggc 2340
ggcttcaact tcagccagat cctgcccgac cccctgaagc ccaccaagcg gagcttcatc 2400
gaggacctgc tgttcaacaa ggtgaccctg gccgacgccg gcttcatgaa gcagtacggc 2460
gagtgcctgg gcgacatcaa cgcccgggac ctgatctgcg cccagaagtt caacggcctg 2520
accgtgctgc cccccctgct gaccgacgac atgatcgccg cctacaccgc cgccctggtg 2580
agcggcaccg ccaccgccgg ctggaccttc ggcgccggcg ccgccctgca gatccccttc 2640
gccatgcaga tggcctaccg gttcaacggc atcggcgtga cccagaacgt gctgtacgag 2700
aaccagaagc agatcgccaa ccagttcaac aaggccatca gccagatcca ggagagcctg 2760
accaccacca gcaccgccct gggcaagctg caggacgtgg tgaaccagaa cgcccaggcc 2820
ctgaacaccc tggtgaagca gctgagcagc aacttcggcg ccatcagcag cgtgctgaac 2880
gacatcctga gccggctgga caaggtggag gccgaggtgc agatcgaccg gctgatcacc 2940
ggccggctgc agagcctgca gacctacgtg acccagcagc tgatccgggc cgccgagatc 3000
cgggccagcg ccaacctggc cgccaccaag atgagcgagt gcgtgctggg ccagagcaag 3060
cgggtggact tctgcggcaa gggctaccac ctgatgagct tcccccaggc cgccccccac 3120
ggcgtggtgt tcctgcacgt gacctacgtg cccagccagg agcggaactt caccaccgcc 3180
cccgccatct gccacgaggg caaggcctac ttcccccggg agggcgtgtt cgtgttcaac 3240
ggcaccagct ggttcatcac ccagcggaac ttcttcagcc cccagatcat caccaccgac 3300
aacaccttcg tgagcggcaa ctgcgacgtg gtgatcggca tcatcaacaa caccgtgtac 3360
gaccccctgc agcccgagct ggacagcttc aaggaggagc tggacaagta cttcaagaac 3420
cacaccagcc ccgacgtgga cctgggcgac atcagcggca tcaacgccag cgtggtgaac 3480
atccagaagg agatcgaccg gctgaacgag gtggccaaga acctgaacga gagcctgatc 3540
gacctgcagg agctgggcaa gtacgagcag tacatcaagt ggcccctgga agttctgttc 3600
caggggcccg gaggaggaag tggaggagga agtggctata ttccggaagc gccgcgcgat 3660
ggccaggcgt atgtgcgcaa agatggcgaa tgggtgctgc tgagcacctt tctgggccat 3720
caccatcacc atcactggtc ccaccctcag ttcgagaagt ga 3762
<210> 8
<211> 116
<212> PRT
<213> Vicugna pacos
<400> 8
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Glu
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ser Thr Leu Asn
20 25 30
Val Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
Ala Gln Ile Thr Leu Asp Gly Ser Pro Glu Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Thr Lys Asp Gly Ala Gln Ser Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Phe Cys Lys
85 90 95
Leu Glu Asn Gly Gly Phe Phe Tyr Tyr Trp Gly Gln Gly Thr Gln Val
100 105 110
Thr Val Ser Thr
115
<210> 9
<211> 348
<212> DNA
<213> Vicugna pacos
<400> 9
caggtgcagc tgcaggagtc ggggggaggc ttggtgcagc ctggggagtc tctgagactc 60
tcctgtgcag cctctggaag tatttctacg ttaaatgtca tgggctggta ccgccaggct 120
ccagggaagc agcgcgagtt ggtcgcacag attactcttg atggtagccc tgagtatgca 180
gactccgtga agggccgatt caccatcacc aaggacggcg cccagagcac gttgtatctg 240
caaatgaaca acttgaaacc tgaggacacg gccgtctatt tctgtaaact cgaaaacggc 300
ggattctttt actactgggg ccaggggacc caggtcacgg tctccaca 348
<210> 10
<211> 116
<212> PRT
<213> Vicugna pacos
<400> 10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ser Thr Leu Asn
20 25 30
Val Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
Ala Arg Ile Thr Leu Asp Gly Arg Pro Glu Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Thr Lys Asp Gly Ala Gln Ser Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Phe Cys Lys
85 90 95
Leu Glu Asn Gly Gly Phe Phe Tyr Tyr Trp Gly Gln Gly Thr Gln Val
100 105 110
Thr Val Ser Ser
115
<210> 11
<211> 348
<212> DNA
<213> Vicugna pacos
<400> 11
caggtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggggggtc tctgagactc 60
tcctgtgcag cctctggaag tatttctacg ttaaatgtca tgggctggta ccgccaggct 120
ccagggaagc agcgcgagtt ggtcgcacgg attactcttg atggtagacc tgagtatgca 180
gactccgtga agggccgatt caccatcacc aaggacggcg cccagagcac gttgtatctg 240
caaatgaaca acttgaaacc tgaggacacg gccgtctatt tctgtaaact cgaaaacggc 300
ggattctttt actactgggg ccaggggacc caggtcaccg tctcctcg 348
<210> 12
<211> 116
<212> PRT
<213> Vicugna pacos
<400> 12
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ser Thr Leu Asn
20 25 30
Val Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val
35 40 45
Ala Gln Ile Thr Leu Asp Gly Arg Pro Glu Tyr Ala Asp Ser Val Lys
50 55 60
Gly Arg Phe Thr Ile Thr Lys Asp Gly Ala Gln Ser Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Phe Cys Lys
85 90 95
Leu Glu Asn Gly Gly Phe Phe Tyr Tyr Trp Gly Gln Gly Thr Gln Val
100 105 110
Thr Val Ser Ser
115
<210> 13
<211> 348
<212> DNA
<213> Vicugna pacos
<400> 13
caggtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggggggtc tctgagactc 60
tcctgtgcag cctctggaag tatttctacg ttaaatgtca tgggctggta ccgccaggct 120
ccagggaagc agcgcgagtt ggtcgcacag attactcttg atggtagacc tgagtatgca 180
gactccgtga agggccgatt caccatcacc aaggacggcg cccagagcac gttgtatctg 240
caaatgaaca acttgaaacc tgaggacacg gccgtctatt tctgtaaact cgaaaacggc 300
ggattctttt actactgggg ccaggggacc caggtcaccg tctcctcg 348
<210> 14
<211> 126
<212> PRT
<213> Vicugna pacos
<400> 14
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ile Leu Ser Arg Tyr
20 25 30
Arg Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val
35 40 45
Ala Ala Val Ser Trp Ser Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser Cys
85 90 95
Ala Ala Asp Val Gln Asp Tyr Met Gly Tyr Ser Lys Met Tyr Gln Asp
100 105 110
Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120 125
<210> 15
<211> 378
<212> DNA
<213> Vicugna pacos
<400> 15
caggtgcagc tggtggagtc ggggggagga ttggtacagg ctgggggctc tctgagactc 60
tcctgtgcag cctctggacg cattttgagt agatatcgca tgggctggtt ccgccaggct 120
ccagggaagg agcgtgagtt tgtagcagcc gttagttgga gtgatggtag cacatactat 180
gcagactcag tgaagggccg attcaccatc tccagagaca acgccaagaa cacggtgtat 240
ctgcagatga acagcctgaa gcctgaggac acggccgttt attcctgtgc agcagatgtg 300
caagactata tgggttactc caagatgtac caagactatg actactgggg ccaggggacc 360
caggtcaccg tctcctcg 378
<210> 16
<211> 1876
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 60
cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 120
atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 180
tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 240
cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 300
tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 360
cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 420
ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 480
aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 540
gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 600
tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc 660
attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg 720
cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt atgtatcata 780
cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc tccacaggtg 840
tccactccca ggtccaactg cacctcggtt ctatcgattg aattccacca tgggatggtc 900
atgtatcatc ctttttctag tagcaactgc aaccggtgta cattctgcta gccgcggccg 960
cggtggtggt ggttctgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc 1020
agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac 1080
cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga 1140
ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa 1200
gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca 1260
ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc 1320
ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac 1380
cctgccccca tcccgggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa 1440
aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa 1500
ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct atagcaagct 1560
caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga 1620
ggctctgcac aaccactaca cgcagaagag cctctccctg tccccgggta aatgagtgcg 1680
acggccggca agcccccgct ccccgggctc tcgcggtcgt acgaggaaag cttggccgcc 1740
atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 1800
tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 1860
tgtatcttat catgta 1876
<210> 17
<211> 1281
<212> PRT
<213> SARS-CoV-2
<400> 17
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr Trp Ser His Pro Gln Phe Glu
1265 1270 1275 1280
Lys
<210> 18
<211> 3846
<212> DNA
<213> SARS-CoV-2
<400> 18
atgttcgtgt tcctggtgct gctgcctctg gtgagcagcc agtgcgtgaa tctgaccacc 60
agaacccagc tgcctcctgc ctacaccaat agcttcacca gaggagttta ttatcccgat 120
aaggtgttca gaagtagtgt attacatagt acccaggacc tgttcctacc tttcttcagt 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcaccaa gagattcgac 240
aatcctgtgc tgcctttcaa tgacggcgtg tacttcgcca gcaccgagaa gagcaatatc 300
atcagaggct ggatcttcgg caccaccttg gattccaaga ctcagagcct gctgattgta 360
aacaacgcta caaatgtggt gatcaaggtg tgcgagttcc agttctgcaa tgaccctttc 420
ctgggtgttt attatcataa gaacaacaag agctggatgg agagcgagtt ccgcgtatat 480
tcgtcggcta ataattgcac cttcgagtac gtgagccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaatctgaga gagttcgtgt tcaagaatat cgacggctac 600
ttcaagatct acagcaagca cacacccatt aatctggtga gagacctgcc tcagggcttc 660
agcgccctgg agcctctggt ggacctgcct atcggcatca atatcaccag attccagacc 720
ctgctggccc tgcacagatc atatcttaca ccaggcgatt cgtcaagcgg ttggaccgct 780
ggagctgcgg catattacgt gggctacctg cagcctagaa ccttcctgct gaagtacaat 840
gagaatggta cgataaccga cgcagttgat tgtgccctgg accctctgag cgagaccaag 900
tgcaccctga agagcttcac cgtggagaag ggcatctacc agaccagcaa tttcagagtg 960
cagcctaccg agagcatcgt gagattccct aatatcacca atctgtgccc tttcggcgag 1020
gtgttcaatg ccaccagatt cgccagcgtg tacgcatgga accgcaagcg gataagcaat 1080
tgcgtggccg actacagcgt gctgtacaat agcgccagct tcagcacctt caaatgttat 1140
ggtgtttcgc caacaaagct gaatgacctg tgcttcacca atgtgtacgc cgacagcttc 1200
gtgatcagag gcgacgaggt gagacagatc gcgccagggc agaccggcaa gatcgccgac 1260
tacaattaca agctgcctga cgacttcacc ggctgcgtga tcgcgtggaa ctctaacaat 1320
ctagattcga aagttggagg caattacaat tacctgtaca gactgttcag aaagagcaat 1380
ctgaagcctt tcgagagaga catcagcacc gagatctacc aggccggcag cacaccgtgt 1440
aatggcgtgg agggcttcaa ttgctacttc cctctgcaga gctacggctt ccagcctacc 1500
aatggcgtgg gctaccagcc ttacagagtg gtggtgctga gcttcgagct gctgcacgct 1560
cccgctaccg tgtgcggccc taagaagagc accaatctgg tgaagaataa gtgcgtgaat 1620
ttcaatttca atggtctaac tggaacgggc gtgctgaccg agagcaataa gaagtttctt 1680
ccctttcaac aattcggcag agacatcgcc gacaccacag atgctgtaag agaccctcag 1740
accctggaga tcctggacat cactccgtgt agcttcggcg gcgtgagcgt gatcacaccg 1800
ggtaccaata ccagcaatca ggtggccgtg ctgtaccagg acgtgaattg caccgaggtg 1860
cctgtggcca tccacgccga ccagctgact cccacttgga gggtatattc cacgggaagc 1920
aatgtgttcc agaccagagc cggctgcctg atcggcgccg agcacgtgaa taatagctac 1980
gagtgcgaca tccctatcgg cgccggcatc tgcgccagct accagaccca gaccaatagc 2040
cctagaagag ccagaagcgt ggccagccag agcatcatcg cctacaccat gagcctgggc 2100
gccgagaata gcgtggccta cagcaataat agcatcgcca tccctaccaa tttcaccatc 2160
agcgtgacca ccgaaatatt accagtctcc atgaccaaga ccagcgtgga ctgcaccatg 2220
tacatctgcg gcgacagcac cgagtgcagc aatctgctgc tgcagtacgg cagcttctgc 2280
acccagctga atagagccct gaccggcatc gccgtggagc aggacaagaa tacccaggag 2340
gtgttcgccc aggtgaagca gatctacaag actccgccga tcaaggactt cggcggcttc 2400
aatttcagcc aaatactccc agatccaagc aagcctagca agaggagctt catcgaggac 2460
ctgctgttca ataaggtgac cctggccgac gccggcttca tcaagcagta cggcgactgc 2520
ctaggtgata ttgcggcaag agacctgatc tgcgcccaga agtttaacgg tttgacagta 2580
ctacctcctc tgctgaccga cgagatgata gcacaatata cgtcggcatt gctcgctggc 2640
acgatcacat cgggctggac tttcggcgcc ggagcagcgt tgcaaatccc tttcgccatg 2700
cagatggcct acagattcaa tggcatcggc gtgacccaga atgtgctgta cgagaatcag 2760
aagctgatcg ccaatcagtt caatagcgcc atcggcaaga tccaggacag cctgagcagc 2820
accgccagcg ccctgggcaa gctgcaggac gtggtgaatc agaatgccca ggccctgaat 2880
accctggtga agcagctgag cagcaatttc ggcgccatca gtagtgtact caacgatatc 2940
ctgagcagac tggacaaggt ggaggccgag gtgcaaattg atcgtcttat tactggcaga 3000
ctgcagagcc tgcagaccta cgtgacccag cagctgatca gagccgccga gatcagagcc 3060
agcgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagag caagagagtg 3120
gacttctgcg gcaagggcta ccacctgatg agcttccctc agagcgctcc acatggcgtg 3180
gtgttcctgc acgtgaccta cgtgcctgcc caggagaaga atttcaccac cgcacccgca 3240
atctgccacg acggcaaggc ccacttccct agagagggcg tgttcgtgag caatggcacc 3300
cactggttcg tgacccagag aaatttctac gagcctcaga tcatcaccac cgacaatacc 3360
ttcgtgagcg gcaattgcga cgtggtgatc gggatagtca ataatactgt ctacgaccct 3420
ctgcagcctg agctggacag cttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccctgacg tggacctcgg tgatatttcg ggaatcaatg ccagcgtggt gaatatccag 3540
aaggaaattg atcggctcaa cgaagtggcc aagaatctga atgagagcct gatcgacctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggcctt ggtacatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgcat gacctcctgt 3720
tgttcctgtt tgaaagggtg ttgttcgtgt gggtcctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ggagccaccc tcagttcgag 3840
aagtga 3846
<210> 19
<211> 3813
<212> DNA
<213> SARS-CoV-2
<400> 19
atgttcgtgt tcctggtgct gctgcccctg gtgagcagcc aatgcgtgaa cctgaccaca 60
agaacacagc tgccccccgc ctacaccaac agcttcacaa gaggcgtgta ctaccccgac 120
aaggtgttca gaagcagcgt gctgcacagc acccaagacc tgttcctgcc cttcttcagc 180
aacgtgacct ggttccacgt gatcagcggc accaacggca ccaagagatt cgacaacccc 240
gtgctgccct tcaacgacgg cgtgtacttc gctagcatcg agaagagcaa catcatcaga 300
ggctggatct tcggcaccac cctggacagc aaaacacaga gcctgctgat cgtgaacaac 360
gccaccaacg tggtgatcaa ggtgtgcgag tttcagttct gcaacgaccc cttcttcgac 420
cacaagaaca acaagagctg gatggagagc gagttcagag tgtacagcag cgccaacaac 480
tgcaccttcg agtacgtgag ccaacccttc ctgatggacc tggagggcaa gcaaggcaac 540
ttcaagaacc tgagagagtt cgtgttcaag aacatcgacg gctacttcaa gatctacagc 600
aagcacaccc ccatcatcgt gagagagccc gaggacctgc cccaaggctt cagcgccctg 660
gagcccctgg tggacctgcc catcggcatc aacatcacaa gatttcagac cctgctggcc 720
ctgcacagat cctacctcac ccctggcgac agcagcagcg ggtggacagc tggcgctgcc 780
gcctactacg tgggctacct gcagcctaga accttcctgc tgaagtacaa cgagaacggc 840
accatcaccg acgccgtgga ctgcgccctg gaccccctga gcgagaccaa gtgcaccctg 900
aagagcttca ccgtggagaa gggcatctat cagacaagca acttcagagt gcagcccacc 960
gagagcatcg tgagattccc caacatcacc aacctgtgcc ccttcgacga ggtgttcaac 1020
gccacaagat tcgctagcgt gtacgcctgg aaccggaaga gaatcagcaa ctgcgtggcc 1080
gactacagcg tgctgtacaa cctggccccc ttcttcacct tcaagtgcta cggcgtgagc 1140
cccaccaagc tgaacgacct gtgcttcacc aacgtgtacg ccgacagctt cgtgatcaga 1200
ggcgacgagg tgagacagat cgcccccggg cagaccggca acatcgccga ctacaactac 1260
aagctgcccg acgacttcac cggctgcgtg atcgcctgga acagcaacaa gctggacagc 1320
aaggtgtccg gcaactacaa ctacctgtac agactgttca gaaagagcaa cctgaagccc 1380
ttcgagagag acatcagcac cgagatctac caagccggca acaagccctg caacggcgtg 1440
gccggcttca actgctactt ccccctgaga agctacagct tcagacccac ctacggcgtg 1500
ggccatcagc cctacagagt ggtcgtgctg agcttcgagc tgctgcacgc ccccgccacc 1560
gtgtgcggcc ccaagaagag caccaacctg gtgaagaaca agtgcgtgaa cttcaacttc 1620
aacggcctga agggcaccgg cgtgctgacc gagagcaaca agaagttcct gccctttcag 1680
cagttcggca gagacatcgc cgacaccacc gacgccgtga gagaccctca gaccctggag 1740
atcctggaca tcaccccctg cagcttcggc ggcgtgagcg tgatcacccc cggcaccaac 1800
acaagcaacc aagtggccgt gctgtaccaa ggcgtgaact gcaccgaggt gcccgtggcc 1860
atccacgccg atcagctgac ccccacctgg agagtgtaca gcaccggcag caacgtgttt 1920
cagacaagag ccggctgcct gatcggcgcc gagtacgtga acaacagcta cgagtgcgac 1980
atccccatcg gcgccggcat ctgcgctagc tatcagacac agaccaagag ccacggcagt 2040
gctagcagcg tggctagcca aagcatcatc gcctacacca tgagcctggg cgccgagaac 2100
agcgtggcct acagcaacaa cagcatcgcc atccccacca acttcaccat cagcgtgacc 2160
accgagatcc tgcccgtcag catgaccaag acaagcgtgg actgcaccat gtacatctgc 2220
ggcgacagca ccgagtgcag caacctgctc ctgcagtacg gcagcttctg cacacagctg 2280
aagagagccc tgaccggcat cgccgtggag caagacaaga acacccaaga ggtgttcgcc 2340
caagtgaagc agatctacaa gacccccccc atcaagtact tcggcggctt caacttcagc 2400
caaattctgc ctgaccctag caagcctagc aagagaagct tcatcgagga cctgctgttc 2460
aacaaggtga ccctggccga cgccggcttc atcaagcagt acggcgactg cctgggcgac 2520
atcgccgcta gagacctgat ctgcgctcag aagttcaagg gcctgaccgt gctgcccccc 2580
ctgctgaccg acgagatgat cgctcagtat acaagcgccc tgctcgccgg gacaatcacc 2640
tccggctgga cctttggcgc cggcgctgcc ctgcagatcc ccttcgccat gcagatggcc 2700
tacagattca acggcatcgg cgtgacacag aacgtgctgt acgagaatca gaagctgatc 2760
gccaatcagt tcaacagcgc catcggcaag atccaagaca gcctgagcag caccgctagc 2820
gccctgggca agctgcaaga cgtggtgaac cacaacgccc aagccctgaa caccctggtg 2880
aagcagctga gcagcaagtt cggcgccatc agcagcgtgc tgaacgacat cttcagcaga 2940
ctggaccctc cagaggccga ggtgcagatc gacagactga tcaccggcag actgcagagc 3000
ctgcagacct acgtgaccca gcagctgatc agagccgccg agatcagagc cagcgccaat 3060
ctggccgcca ccaagatgag cgagtgcgtg ctgggccaga gcaagagagt ggacttctgc 3120
ggcaagggct accacctgat gagcttccct cagagcgctc cacatggcgt ggtgttcctg 3180
cacgtgacct acgtgcctgc ccaggagaag aatttcacca ccgcacccgc aatctgccac 3240
gacggcaagg cccacttccc tagagagggc gtgttcgtga gcaatggcac ccactggttc 3300
gtgacccaga gaaatttcta cgagcctcag atcatcacca ccgacaatac cttcgtgagc 3360
ggcaattgcg acgtggtgat cgggatagtc aataatactg tctacgaccc tctgcagcct 3420
gagctggaca gcttcaagga ggagctggac aagtacttca agaatcacac cagccctgac 3480
gtggacctcg gtgatatttc gggaatcaat gccagcgtgg tgaatatcca gaaggaaatt 3540
gatcggctca acgaagtggc caagaatctg aatgagagcc tgatcgacct gcaggagctg 3600
ggcaagtacg agcagtacat caagtggcct tggtacatct ggctgggctt catcgccggc 3660
ctgatcgcca tcgtgatggt gaccatcatg ctgtgctgca tgacctcctg ttgttcctgt 3720
ttgaaagggt gttgttcgtg tgggtcctgc tgcaagttcg acgaggacga cagcgagcct 3780
gtgctgaagg gcgtgaagct gcactacacc tga 3813
<210> 20
<211> 3813
<212> DNA
<213> SARS-CoV-2
<400> 20
atgttcgtgt tcctggtatt gctgccgctg gtgagctctc agtgcgtgaa ccttatcacc 60
agaacccaga gctacaccaa cagcttcacc cggggtgttt actaccccga caaagtgttc 120
cggagctctg ttctgcatag cacccaagac ctgttcctgc ctttcttctc taacgtgacc 180
tggttccacg ccatccacgt gtctggaaca aacggaacca aaagatttga caaccccgtc 240
ctgcctttta atgacggagt gtatttcgcc tccacagaaa agagcaacat catcagaggc 300
tggatctttg gcaccactct ggattccaag acccagagcc tgctgatcgt gaacaacgcc 360
acaaacgtcg tgatcaaggt ctgcgagttc cagttctgta acgatccttt tctggacgtg 420
tactaccaca agaacaacaa gagctggatg gaatctgagt ttcgggtgta cagcagcgct 480
aataattgca ccttcgagta cgtttcccag ccattcctga tggacctgga gggcaagcag 540
ggaaacttca agaacctcag agagttcgtg ttcaaaaaca tcgacggcta cttcaagatc 600
tacagcaagc acaccccaat caacctgggc agagacctgc ctcagggctt tagcgccctg 660
gaaccactgg tggatctccc tatcggcatc aacatcacac ggtttcagac cctgctggcc 720
ctccacagaa gctatctgac gcccggcgac agtagctccg gatggaccgc gggcgccgcc 780
gcttactacg tgggctatct gcagcctaga acattcctgt tgaagtacaa cgagaacggg 840
accatcacag atgccgtgga ctgcgccctg gaccctctga gcgagacaaa gtgcaccctg 900
aagagcttca ccgtggaaaa gggcatctac caaaccagca acttcagagt gcagcctaca 960
gagagcattg tcagattccc caacatcacc aatctgtgcc catttgatga ggtgttcaac 1020
gccacccggt tcgccagcgt gtacgcctgg aatagaaaga gaatctccaa ttgcgtggct 1080
gactacagcg tgctgtacaa cttcgctccc ttcttcgcct tcaagtgcta cggcgtctcc 1140
cctacgaagc tgaacgacct ctgtttcaca aatgtgtacg ccgatagctt cgtgatccgg 1200
ggcaatgagg ttagccagat cgcacccggc cagactggca acatcgcgga ttacaactac 1260
aaactgcccg atgacttcac aggctgcgtg atcgcctgga acagcaacaa gctggacagc 1320
aaggtgggag gtaactacaa ctacttgtac cggctgtttc ggaagtctaa ccttaaacct 1380
tttgagagag acatctctac cgagatctac caagccggaa ataaaccttg caacggcgtg 1440
gccggattca actgctactt tcctctgaga agctacggct tcagacctac gtacggcgtt 1500
ggccaccaac cttaccgcgt ggtggttctg agctttgaac tgctgcacgc ccctgccacc 1560
gtgtgcggcc caaagaaaag taccaaccta gtcaagaaca aatgcgtgaa cttcaatttc 1620
aacggcctga ccggcacagg cgtgctgact gagagcaaca agaaattcct gcctttccaa 1680
cagttcggca gagatattgc tgacaccacc gacgccgtgc gggaccccca gaccctggaa 1740
atcctggata tcaccccttg ttcttttgga ggcgtgagcg tgatcactcc tggaaccaac 1800
acgtccaatc aggtggccgt gctgtatcag ggcgtgaact gcaccgaggt gcccgtggcc 1860
atccacgccg accagctgac ccctacatgg cgggtgtaca gcacaggaag caatgtgttc 1920
cagaccagag ccggctgtct gataggagct gaatacgtga acaattctta cgagtgtgac 1980
attcccatcg gcgccggcat ctgtgcctcc taccagaccc agacaaagag ccaccggaga 2040
gccagaagcg tcgccagcca gtccatcatc gcttatacca tgagcctcgg cgctgaaaac 2100
tccgtggcct acagcaacaa cagcatcgcc atccccacca actttacaat cagcgtcaca 2160
accgaaatcc tgcccgtgag catgacgaag acctctgtgg actgtaccat gtacatctgc 2220
ggcgacagca ccgagtgctc caatctgctg ctgcagtacg gctctttttg cacacagctg 2280
aagagagcac tgaccggaat tgctgtggaa caggacaaga acacccagga ggtgttcgcc 2340
caggtgaagc agatctataa gacacctcca atcaagtact tcggcggctt caactttagc 2400
cagatcctgc ccgaccccag caagccttct aaacgcagct tcattgagga cctgctgttt 2460
aacaaggtga ccctggccga cgctggtttc atcaagcagt acggcgattg cctgggcgac 2520
atcgcggctc gggacctgat ctgcgcccag aagttcaacg gcctgacagt gctgcctcct 2580
ctgctcacag atgagatgat cgcccagtac accagcgccc tgctcgccgg tacaatcaca 2640
tccggctgga ccttcggcgc tggcgctgcc ctgcaaatcc ctttcgcaat gcagatggcc 2700
tacagattca atggaatcgg cgtcacccag aacgtgctgt acgagaacca gaagctgatc 2760
gccaatcaat tcaacagcgc catcggcaag atccaggatt ccctgagctc taccgccagc 2820
gccctgggca agctgcagga cgtggtgaac cacaacgccc aggccctgaa cacactggtg 2880
aaacagctgt cttctaaatt cggcgccatt tcatccgtgc ttaatgacat cctgtctaga 2940
ctggacaaag tggaagccga agtccagatc gacaggctga ttacaggaag actgcaaagc 3000
ctacagacct acgtgaccca gcaactgatc agagccgctg agatcagagc ctctgccaac 3060
ctggcagcca ccaagatgag cgagtgcgtg ctgggacagt ctaagagggt ggatttctgc 3120
ggaaagggtt atcacctgat gagcttcccc caaagcgccc ctcacggcgt ggtgttcctg 3180
catgtgactt acgtgccagc tcaggagaag aacttcacaa ccgcccctgc catctgccac 3240
gacggcaagg cccatttccc tagagaaggc gttttcgtga gcaatggcac ccactggttc 3300
gtgacccaga ggaacttcta cgagccccag atcatcacca ccgataatac tttcgtgagt 3360
ggcaattgtg acgtggtgat cggcatcgtg aacaacaccg tgtacgaccc tctgcagcct 3420
gagctggata gctttaagga ggaactggat aagtacttca aaaaccacac aagcccggac 3480
gtggacctgg gcgacatcag cggcataaac gccagcgtgg tgaacatcca gaaagaaatc 3540
gacagactga acgaagtggc caagaacctg aatgagagcc tgatcgatct gcaggagctg 3600
ggaaagtacg agcagtacat caagtggcct tggtacatct ggctgggatt catcgccggc 3660
ctgatcgcta tcgtgatggt gactattatg ctgtgttgca tgaccagttg ttgtagctgc 3720
ctgaagggct gctgcagctg tggcagctgc tgtaaattcg acgaggatga tagtgaacct 3780
gtgctgaagg gcgtgaagtt gcactacacc tga 3813
<210> 21
<211> 1255
<212> PRT
<213> SARS-CoV-1
<400> 21
Met Phe Ile Phe Leu Leu Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu
1 5 10 15
Asp Arg Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Thr Gln
20 25 30
His Thr Ser Ser Met Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg
35 40 45
Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser
50 55 60
Asn Val Thr Gly Phe His Thr Ile Asn His Thr Phe Gly Asn Pro Val
65 70 75 80
Ile Pro Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu Lys Ser Asn
85 90 95
Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln
100 105 110
Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys
115 120 125
Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Met
130 135 140
Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr
145 150 155 160
Phe Glu Tyr Ile Ser Asp Ala Phe Ser Leu Asp Val Ser Glu Lys Ser
165 170 175
Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly
180 185 190
Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp
195 200 205
Leu Pro Ser Gly Phe Asn Thr Leu Lys Pro Ile Phe Lys Leu Pro Leu
210 215 220
Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Ser Pro
225 230 235 240
Ala Gln Asp Ile Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr
245 250 255
Leu Lys Pro Thr Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile
260 265 270
Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys
275 280 285
Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn
290 295 300
Phe Arg Val Val Pro Ser Gly Asp Val Val Arg Phe Pro Asn Ile Thr
305 310 315 320
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Lys Phe Pro Ser
325 330 335
Val Tyr Ala Trp Glu Arg Lys Lys Ile Ser Asn Cys Val Ala Asp Tyr
340 345 350
Ser Val Leu Tyr Asn Ser Thr Phe Phe Ser Thr Phe Lys Cys Tyr Gly
355 360 365
Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala
370 375 380
Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly
385 390 395 400
Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe
405 410 415
Met Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Ser
420 425 430
Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Tyr Leu Arg His Gly Lys Leu
435 440 445
Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly
450 455 460
Lys Pro Cys Thr Pro Pro Ala Leu Asn Cys Tyr Trp Pro Leu Asn Asp
465 470 475 480
Tyr Gly Phe Tyr Thr Thr Thr Gly Ile Gly Tyr Gln Pro Tyr Arg Val
485 490 495
Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly
500 505 510
Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn
515 520 525
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg
530 535 540
Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Ser Asp Phe Thr Asp
545 550 555 560
Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys
565 570 575
Ala Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Ala Ser Ser
580 585 590
Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Ser Thr
595 600 605
Ala Ile His Ala Asp Gln Leu Thr Pro Ala Trp Arg Ile Tyr Ser Thr
610 615 620
Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu
625 630 635 640
His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile
645 650 655
Cys Ala Ser Tyr His Thr Val Ser Leu Leu Arg Ser Thr Ser Gln Lys
660 665 670
Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala
675 680 685
Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile
690 695 700
Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys
705 710 715 720
Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu
725 730 735
Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile
740 745 750
Ala Ala Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys
755 760 765
Gln Met Tyr Lys Thr Pro Thr Leu Lys Tyr Phe Gly Gly Phe Asn Phe
770 775 780
Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile
785 790 795 800
Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met
805 810 815
Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile
820 825 830
Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr
835 840 845
Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala
850 855 860
Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe
865 870 875 880
Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn
885 890 895
Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala
900 905 910
Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly
915 920 925
Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu
930 935 940
Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn
945 950 955 960
Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp
965 970 975
Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln
980 985 990
Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala
995 1000 1005
Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe
1010 1015 1020
Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ala Ala Pro His
1025 1030 1035 1040
Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ser Gln Glu Arg Asn
1045 1050 1055
Phe Thr Thr Ala Pro Ala Ile Cys His Glu Gly Lys Ala Tyr Phe Pro
1060 1065 1070
Arg Glu Gly Val Phe Val Phe Asn Gly Thr Ser Trp Phe Ile Thr Gln
1075 1080 1085
Arg Asn Phe Phe Ser Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val
1090 1095 1100
Ser Gly Asn Cys Asp Val Val Ile Gly Ile Ile Asn Asn Thr Val Tyr
1105 1110 1115 1120
Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys
1125 1130 1135
Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser
1140 1145 1150
Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu
1155 1160 1165
Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu
1170 1175 1180
Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Val Trp Leu
1185 1190 1195 1200
Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Leu Leu
1205 1210 1215
Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Ala Cys Ser Cys
1220 1225 1230
Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu Lys
1235 1240 1245
Gly Val Lys Leu His Tyr Thr
1250 1255
<210> 22
<211> 3768
<212> DNA
<213> SARS-CoV-1
<400> 22
atgttcatct tcctgctgtt cctgaccctg accagcggca gcgacctgga ccggtgcacc 60
accttcgacg acgtgcaggc ccccaactac acccagcaca ccagcagcat gcggggcgtg 120
tactaccccg acgagatctt ccggagcgac accctgtacc tgacccagga cctgttcctg 180
cccttctaca gcaacgtgac cggcttccac accatcaacc acaccttcgg caaccccgtg 240
atccccttca aggacggcat ctacttcgcc gccaccgaga agagcaacgt ggtgcggggc 300
tgggtgttcg gcagcaccat gaacaacaag agccagagcg tgatcatcat caacaacagc 360
accaacgtgg tgatccgggc ctgcaacttc gagctgtgcg acaacccctt cttcgccgtg 420
agcaagccca tgggcaccca gacccacacc atgatcttcg acaacgcctt caactgcacc 480
ttcgagtaca tcagcgacgc cttcagcctg gacgtgagcg agaagagcgg caacttcaag 540
cacctgcggg agttcgtgtt caagaacaag gacggcttcc tgtacgtgta caagggctac 600
cagcccatcg acgtggtgcg ggacctgccc agcggcttca acaccctgaa gcccatcttc 660
aagctgcccc tgggcatcaa catcaccaac ttccgggcca tcctgaccgc cttcagcccc 720
gcccaggaca tctggggcac cagcgccgcc gcctacttcg tgggctacct gaagcccacc 780
accttcatgc tgaagtacga cgagaacggc accatcaccg acgccgtgga ctgcagccag 840
aaccccctgg ccgagctgaa gtgcagtgtg aagagcttcg agatcgacaa gggcatctac 900
cagaccagca acttccgggt ggtgcccagc ggcgacgtgg tgcggttccc caacatcacc 960
aacctgtgcc ccttcggcga ggtgttcaac gccaccaagt tccccagcgt gtacgcctgg 1020
gagcggaaga agatcagcaa ctgcgtggcc gactacagcg tgctgtacaa cagcaccttc 1080
ttcagcacct tcaagtgcta cggcgtgagc gccaccaagc tgaacgacct gtgcttcagc 1140
aacgtgtacg ccgacagctt cgtggtgaag ggcgacgacg tgcggcagat cgcccccggc 1200
cagaccggcg tgatcgccga ctacaactac aagctgcccg acgacttcat gggctgcgtg 1260
ctggcctgga acacccggaa catcgacgcc accagcaccg gcaactacaa ctacaagtac 1320
cggtacctgc ggcacggcaa gctgcggccc ttcgagcggg acatcagcaa cgtgcccttc 1380
agccccgacg gcaagccctg cacccccccc gccctgaact gctactggcc cctgaacgac 1440
tacggcttct acaccactac cggcatcggc taccagccct accgggtggt ggtgctgagc 1500
ttcgagctgc tgaacgcccc cgccaccgtg tgcggcccca agctgagcac cgacctgatc 1560
aagaaccagt gcgtgaactt caacttcaac ggcctgaccg gcaccggcgt gctgaccccc 1620
agcagcaagc ggttccagcc cttccagcag ttcggccggg acgtgagcga cttcaccgac 1680
agcgtgcggg accccaagac cagcgagatc ctggacatca gcccctgcgc cttcggcggc 1740
gtgagcgtga tcacccccgg taccaacgcc agcagcgagg tggccgtgct gtaccaggac 1800
gtgaactgca ccgacgtgag caccgccatc cacgccgacc agctgacccc cgcctggcgg 1860
atctacagca ccggcaacaa cgtgttccag acccaggccg gctgcctgat cggcgccgag 1920
cacgtggaca ccagctacga gtgcgacatc cccatcggcg ccggcatctg cgccagctac 1980
cacaccgtga gcctgctgcg gagcaccagc cagaagagca tcgtggccta caccatgagc 2040
ctgggcgccg acagcagcat cgcctacagc aacaacacca tcgccatccc caccaacttc 2100
agcatcagca tcaccaccga ggtgatgccc gtgagcatgg ccaagaccag cgtggactgc 2160
aacatgtaca tctgcggcga cagcaccgaa tgcgccaacc tgctgctgca gtacggcagc 2220
ttctgcaccc aactgaaccg ggccctgagc ggcatcgccg ccgagcagga ccggaacacc 2280
cgggaggtgt tcgcccaggt gaagcagatg tacaagaccc ccaccctgaa gtacttcggc 2340
ggcttcaact tcagccagat cctgcccgac cccctgaagc ccaccaagcg gagcttcatc 2400
gaggacctgc tgttcaacaa ggtgaccctg gccgacgccg gcttcatgaa gcagtacggc 2460
gagtgcctgg gcgacatcaa cgcccgggac ctgatctgcg cccagaagtt caacggcctg 2520
accgtgctgc cccccctgct gaccgacgac atgatcgccg cctacaccgc cgccctggtg 2580
agcggcaccg ccaccgccgg ctggaccttc ggcgccggcg ccgccctgca gatccccttc 2640
gccatgcaga tggcctaccg gttcaacggc atcggcgtga cccagaacgt gctgtacgag 2700
aaccagaagc agatcgccaa ccagttcaac aaggccatca gccagatcca ggagagcctg 2760
accaccacca gcaccgccct gggcaagctg caggacgtgg tgaaccagaa cgcccaggcc 2820
ctgaacaccc tggtgaagca gctgagcagc aacttcggcg ccatcagcag cgtgctgaac 2880
gacatcctga gccggctgga caaggtggag gccgaggtgc agatcgaccg gctgatcacc 2940
ggccggctgc agagcctgca gacctacgtg acccagcagc tgatccgggc cgccgagatc 3000
cgggccagcg ccaacctggc cgccaccaag atgagcgagt gcgtgctggg ccagagcaag 3060
cgggtggact tctgcggcaa gggctaccac ctgatgagct tcccccaggc cgccccccac 3120
ggcgtggtgt tcctgcacgt gacctacgtg cccagccagg agcggaactt caccaccgcc 3180
cccgccatct gccacgaggg caaggcctac ttcccccggg agggcgtgtt cgtgttcaac 3240
ggcaccagct ggttcatcac ccagcggaac ttcttcagcc cccagatcat caccaccgac 3300
aacaccttcg tgagcggcaa ctgcgacgtg gtgatcggca tcatcaacaa caccgtgtac 3360
gaccccctgc agcccgagct ggacagcttc aaggaggagc tggacaagta cttcaagaac 3420
cacaccagcc ccgacgtgga cctgggcgac atcagcggca tcaacgccag cgtggtgaac 3480
atccagaagg agatcgaccg gctgaacgag gtggccaaga acctgaacga gagcctgatc 3540
gacctgcagg agctgggcaa gtacgagcag tacatcaagt ggccctggta cgtgtggctg 3600
ggcttcatcg ccggcctgat cgccatcgtg atggtgacca tcctgctgtg ctgcatgacc 3660
agctgctgca gctgcctgaa gggcgcctgc agctgcggca gctgctgcaa gttcgacgag 3720
gacgacagcg agcccgtgct gaagggcgtg aagctgcact acacctga 3768
<210> 23
<211> 1265
<212> PRT
<213> Pangolin CoV GD
<400> 23
Met Leu Phe Phe Phe Phe Leu His Phe Ala Leu Val Asn Ser Gln Cys
1 5 10 15
Val Asn Leu Thr Gly Arg Ala Ala Ile Gln Pro Ser Phe Thr Asn Ser
20 25 30
Ser Gln Arg Gly Val Tyr Tyr Pro Asp Thr Ile Phe Arg Ser Asn Thr
35 40 45
Leu Val Leu Ser Gln Gly Tyr Phe Leu Pro Phe Tyr Ser Asn Val Ser
50 55 60
Trp Tyr Tyr Ala Leu Thr Lys Thr Asn Ser Ala Glu Lys Arg Val Asp
65 70 75 80
Asn Pro Val Leu Asp Phe Lys Asp Gly Ile Tyr Phe Ala Ala Thr Glu
85 90 95
Lys Ser Asn Ile Val Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Asn
100 105 110
Thr Ser Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Ile Ile
115 120 125
Lys Val Cys Asn Phe Gln Phe Cys Tyr Asp Pro Tyr Leu Ser Gly Tyr
130 135 140
Tyr His Asn Asn Lys Thr Trp Ser Thr Arg Glu Phe Ala Val Tyr Ser
145 150 155 160
Ser Tyr Ala Asn Cys Thr Phe Glu Tyr Val Ser Lys Ser Phe Met Leu
165 170 175
Asp Ile Ala Gly Lys Ser Gly Leu Phe Asp Thr Leu Arg Glu Phe Val
180 185 190
Phe Arg Asn Val Asp Gly Tyr Phe Lys Ile Tyr Ser Lys Tyr Thr Pro
195 200 205
Val Asn Val Asn Ser Asn Leu Pro Ile Gly Phe Ser Ala Leu Glu Pro
210 215 220
Leu Val Glu Ile Pro Ala Gly Ile Asn Ile Thr Lys Phe Arg Thr Leu
225 230 235 240
Leu Thr Ile His Arg Gly Asp Pro Met Pro Asn Asn Gly Trp Thr Val
245 250 255
Phe Ser Ala Ala Tyr Tyr Val Gly Tyr Leu Ala Pro Arg Thr Phe Met
260 265 270
Leu Asn Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala
275 280 285
Leu Asp Pro Leu Ser Glu Ala Lys Cys Thr Leu Lys Ser Leu Thr Val
290 295 300
Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu
305 310 315 320
Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu
325 330 335
Val Phe Asn Ala Thr Thr Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys
340 345 350
Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Thr
355 360 365
Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn
370 375 380
Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Val Arg Gly
385 390 395 400
Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Arg Ile Ala Asp
405 410 415
Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp
420 425 430
Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu
435 440 445
Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile
450 455 460
Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu
465 470 475 480
Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe His Pro Thr
485 490 495
Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu
500 505 510
Leu Leu Asn Ala Pro Ala Thr Val Cys Gly Pro Lys Gln Ser Thr Asn
515 520 525
Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly
530 535 540
Thr Gly Val Leu Thr Glu Ser Ser Lys Lys Phe Leu Pro Phe Gln Gln
545 550 555 560
Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln
565 570 575
Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser
580 585 590
Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr
595 600 605
Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln
610 615 620
Leu Thr Pro Thr Trp Ser Val Tyr Ser Thr Gly Ser Asn Val Phe Gln
625 630 635 640
Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr
645 650 655
Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr
660 665 670
Gln Thr Asn Ser Arg Ser Val Ser Ser Gln Ala Ile Ile Ala Tyr Thr
675 680 685
Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ala Asn Asn Ser Ile
690 695 700
Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro
705 710 715 720
Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly
725 730 735
Asp Ser Ile Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys
740 745 750
Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys
755 760 765
Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro
770 775 780
Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp
785 790 795 800
Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn
805 810 815
Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys
820 825 830
Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn
835 840 845
Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln
850 855 860
Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe
865 870 875 880
Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr
885 890 895
Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln
900 905 910
Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp
915 920 925
Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val
930 935 940
Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser
945 950 955 960
Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu
965 970 975
Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg
980 985 990
Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala
995 1000 1005
Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys
1010 1015 1020
Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His
1025 1030 1035 1040
Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His
1045 1050 1055
Val Thr Tyr Val Pro Ser Gln Glu Lys Asn Phe Thr Thr Thr Pro Ala
1060 1065 1070
Ile Cys His Glu Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val
1075 1080 1085
Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro
1090 1095 1100
Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Ser Cys Asp Val
1105 1110 1115 1120
Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu
1125 1130 1135
Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr
1140 1145 1150
Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val
1155 1160 1165
Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn
1170 1175 1180
Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln
1185 1190 1195 1200
Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu
1205 1210 1215
Ile Ala Ile Ile Met Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys
1220 1225 1230
Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe
1235 1240 1245
Asp Glu Asp Asp Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr
1250 1255 1260
Thr
1265
<210> 24
<211> 3798
<212> DNA
<213> Pangolin CoV GD
<400> 24
atgctgttct tcttcttcct gcacttcgcc ctggtgaact cccagtgcgt gaacctgacc 60
ggccgggccg ctatccagcc ctctttcacc aactcctccc agagaggcgt gtactacccc 120
gacaccatct tccgctccaa caccctggtg ctgagccagg gctacttcct gcccttctat 180
agcaatgtga gctggtacta cgccctgacc aagaccaaca gcgctgaaaa gcgcgtggac 240
aaccccgtgc tggacttcaa agacggcatt tacttcgctg ccaccgaaaa atccaacatc 300
gtgagaggct ggatcttcgg aaccaccctg gacaacacct cccagagcct gctgattgtg 360
aacaatgcca ccaacgtgat tatcaaggtg tgcaatttcc agttttgcta cgacccctat 420
ctgagcggct actaccacaa caacaaaacc tggtccacca gagaatttgc cgtgtatagc 480
agctacgcca actgcacctt cgagtacgtg tccaagagct tcatgctgga catcgccggc 540
aagagcggcc tgttcgacac actgagagag tttgtgttca gaaacgtgga cggctacttc 600
aaaatctaca gcaagtacac ccccgtgaac gtgaacagca acctgcccat cggatttagc 660
gccctggagc ccctggtgga gatccccgct ggcatcaaca tcaccaaatt ccgcaccctg 720
ctgaccatcc acagaggcga ccccatgccc aacaacggct ggaccgtgtt ctccgccgcc 780
tactatgtgg gctacctggc ccccagaacc ttcatgctga actacaacga aaacggcacc 840
atcaccgacg ccgtggactg cgccctggac ccactgtctg aggctaagtg taccctgaag 900
agcctgacag tggaaaaagg catctaccag acctccaact tcagagtgca gcctacagaa 960
agcattgtga gatttcccaa catcaccaac ctgtgcccct ttggcgaggt gttcaacgcc 1020
accaccttcg ccagcgtgta cgcctggaac agaaaaagaa tctcaaactg cgtggccgac 1080
tacagcgtgc tgtacaacag cacctccttc tccaccttca agtgctatgg cgtgtccccc 1140
accaagctga acgatctgtg ttttaccaac gtgtacgccg actccttcgt ggtgagaggc 1200
gacgaggtgc gccagatcgc ccccggacag accggaagga tcgccgacta taactacaag 1260
ctgcccgacg acttcaccgg ctgcgtgatc gcctggaact ccaacaatct ggactctaag 1320
gtgggcggca actacaacta cctgtacaga ctgttcagaa aaagcaacct gaagcccttc 1380
gaaagagaca tctccacaga gatctaccag gccggctcca ccccctgcaa cggcgtggaa 1440
ggcttcaact gctacttccc cctgcagagc tacggcttcc accccaccaa cggcgtgggc 1500
taccagccct acagagtggt ggtgctgtcc ttcgagctgc tgaacgcccc cgccacagtg 1560
tgcggcccta agcagtccac caacctggtg aaaaacaaat gcgtgaactt caatttcaac 1620
ggactgaccg ggaccggcgt gctgaccgag agctccaaaa agtttctgcc cttccagcag 1680
ttcggcagag acatcgccga caccacagac gccgtgagag acccccagac cctggagatt 1740
ctggacatta caccctgctc cttcggcggc gtgtccgtga ttacccccgg caccaacacc 1800
agcaaccagg tggccgtgct gtaccaggat gtgaactgca ccgaggtgcc tgtggccatc 1860
cacgccgacc agctgacccc cacctggagt gtgtacagca ccggcagcaa cgtgttccag 1920
accagagccg gatgcctgat cggcgccgag cacgtgaaca acagctacga gtgcgacatt 1980
cccatcggcg ccggcatctg cgccagctac cagacccaga caaacagcag aagcgtgagc 2040
agccaggcca tcatcgccta caccatgagc ctgggcgccg agaacagcgt ggcctacgcc 2100
aacaattcca ttgccatccc caccaacttc accatcagcg tgaccacaga aatcctgccc 2160
gtgtccatga ccaagaccag cgtggactgc accatgtata tctgcggcga ttccattgag 2220
tgctccaacc tgctgctgca gtacggcagc ttctgcaccc agctgaaccg cgccctgact 2280
ggcatcgccg tggagcagga caagaacacc caggaggtgt tcgctcaggt gaagcagatc 2340
tacaaaaccc cccccattaa ggacttcggc ggcttcaact tcagccagat cctgcccgat 2400
ccctccaagc ccagcaagag aagcttcatc gaagatctgc tgttcaataa ggtgaccctg 2460
gccgacgccg gcttcatcaa acagtatgga gactgcctgg gagacatcgc cgccagagac 2520
ctgatctgcg cccagaaatt caacggcctg accgtgctgc cccccctgct gacagacgag 2580
atgatcgctc agtacaccag cgccctgctg gccggcacaa tcacctccgg atggactttt 2640
ggcgccggcg ccgctctgca gatccccttt gccatgcaga tggcctaccg gttcaacggc 2700
atcggagtga cccagaacgt gctgtacgaa aatcagaagc tgatcgccaa ccagttcaac 2760
tccgctatcg gcaagatcca ggacagcctg agcagcaccg ccagcgccct gggaaagctg 2820
caggatgtgg tgaaccagaa cgcccaggcc ctgaacaccc tggtgaaaca gctgagcagc 2880
aacttcggag ccatcagcag cgtgctgaac gacatcctgt ccagactgga caaagtggag 2940
gccgaggtgc agatcgacag actgatcacc ggcagactgc agagcctgca gacctacgtg 3000
acccagcagc tgatcagagc cgccgagatt agagccagcg ccaacctggc cgccaccaag 3060
atgagcgaat gcgtgctggg acagagcaag agagtggact tctgcggaaa gggatatcac 3120
ctgatgagct tcccacagag cgccccccac ggagtggtgt tcctgcacgt gacctacgtg 3180
cctagccagg agaagaattt caccacaaca cctgccatct gccacgaggg caaggcccac 3240
ttcccccgcg aaggcgtgtt tgtgagcaac ggcacccact ggttcgtgac ccagagaaac 3300
ttctacgagc cccagatcat caccaccgac aataccttcg tgagcggaag ctgtgacgtg 3360
gtgatcggaa tcgtgaacaa taccgtgtac gaccccctgc agcccgaact ggacagcttc 3420
aaggaagagc tggacaaata cttcaagaac cacaccagcc cagacgtgga tctgggagat 3480
atcagcggca tcaacgccag cgtggtgaac atccagaagg agatcgacag actgaacgaa 3540
gtggccaaaa acctgaacga gagcctgatc gacctgcagg agctgggaaa gtatgaacag 3600
tacattaagt ggccctggta catctggctg ggcttcattg ccggcctgat cgccatcatt 3660
atggtgacca tcatgctgtg ctgcatgacc agctgctgta gctgcctgaa gggatgctgc 3720
tcctgcggca gctgctgcaa gttcgacgaa gacgactctg agcctgtgct gaagggggtg 3780
aagctgcact acacctga 3798
<210> 25
<211> 1267
<212> PRT
<213> Pangolin CoV GX
<400> 25
Met Phe Val Phe Leu Phe Val Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gly Ile Pro Pro Gly Tyr Thr Asn Ser Ser
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Ile Leu
35 40 45
His Leu Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe Asn Thr Ile Asn Tyr Gln Gly Gly Phe Lys Lys Phe Asp Asn Pro
65 70 75 80
Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser
85 90 95
Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ala Arg Thr
100 105 110
Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val
115 120 125
Cys Glu Phe Gln Phe Cys Thr Asp Pro Phe Leu Gly Val Tyr Tyr His
130 135 140
Asn Asn Asn Lys Thr Trp Val Glu Asn Glu Phe Arg Val Tyr Ser Ser
145 150 155 160
Ala Asn Asn Cys Thr Phe Glu Tyr Ile Ser Gln Pro Phe Leu Met Asp
165 170 175
Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe
180 185 190
Lys Asn Val Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile
195 200 205
Asp Leu Val Arg Asp Leu Pro Arg Gly Phe Ala Ala Leu Glu Pro Leu
210 215 220
Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu
225 230 235 240
Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asn Leu Glu Ser Gly Trp
245 250 255
Thr Thr Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Gln Arg Thr
260 265 270
Phe Leu Leu Ser Tyr Asn Gln Asn Gly Thr Ile Thr Asp Ala Val Asp
275 280 285
Cys Ser Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Leu
290 295 300
Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro
305 310 315 320
Thr Ile Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe
325 330 335
Gly Glu Val Phe Asn Ala Ser Lys Phe Ala Ser Val Tyr Ala Trp Asn
340 345 350
Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn
355 360 365
Ser Thr Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys
370 375 380
Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Val
385 390 395 400
Lys Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Val Ile
405 410 415
Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile
420 425 430
Ala Trp Asn Ser Val Lys Gln Asp Ala Leu Thr Gly Gly Asn Tyr Gly
435 440 445
Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Lys Leu Lys Pro Phe Glu Arg
450 455 460
Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly
465 470 475 480
Gln Val Gly Leu Asn Cys Tyr Tyr Pro Leu Glu Arg Tyr Gly Phe His
485 490 495
Pro Thr Thr Gly Val Asn Tyr Gln Pro Phe Arg Val Val Val Leu Ser
500 505 510
Phe Glu Leu Leu Asn Gly Pro Ala Thr Val Cys Gly Pro Lys Leu Ser
515 520 525
Thr Thr Leu Val Lys Asp Lys Cys Val Asn Phe Asn Phe Asn Gly Leu
530 535 540
Thr Gly Thr Gly Val Leu Thr Thr Ser Lys Lys Gln Phe Leu Pro Phe
545 550 555 560
Gln Gln Phe Gly Arg Asp Ile Ser Asp Thr Thr Asp Ala Val Arg Asp
565 570 575
Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly
580 585 590
Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val
595 600 605
Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Met Ala Ile His Ala
610 615 620
Glu Gln Leu Thr Pro Ala Trp Arg Val Tyr Ser Ala Gly Ala Asn Val
625 630 635 640
Phe Gln Thr Arg Ala Gly Cys Leu Val Gly Ala Glu His Val Asn Asn
645 650 655
Ser Tyr Glu Cys Asp Ile Pro Val Gly Ala Gly Ile Cys Ala Ser Tyr
660 665 670
His Ser Met Ser Ser Leu Arg Ser Val Asn Gln Arg Ser Ile Ile Ala
675 680 685
Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn
690 695 700
Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile
705 710 715 720
Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile
725 730 735
Cys Gly Asp Ser Ile Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser
740 745 750
Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln
755 760 765
Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys
770 775 780
Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu
785 790 795 800
Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu
805 810 815
Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly
820 825 830
Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys
835 840 845
Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile
850 855 860
Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp
865 870 875 880
Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met
885 890 895
Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu
900 905 910
Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile
915 920 925
Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp
930 935 940
Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu
945 950 955 960
Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser
965 970 975
Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr
980 985 990
Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg
995 1000 1005
Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser
1010 1015 1020
Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly
1025 1030 1035 1040
Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe
1045 1050 1055
Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala
1060 1065 1070
Pro Ala Ile Cys His Glu Gly Lys Ala His Phe Pro Arg Glu Gly Val
1075 1080 1085
Phe Val Ser Asn Gly Thr His Trp Phe Ile Thr Gln Arg Asn Phe Tyr
1090 1095 1100
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Ser Cys
1105 1110 1115 1120
Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln
1125 1130 1135
Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn
1140 1145 1150
His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala
1155 1160 1165
Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala
1170 1175 1180
Lys Asn Leu Asn Glu Ser Pro Ile Asp Leu Gln Glu Leu Gly Lys Tyr
1185 1190 1195 1200
Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala
1205 1210 1215
Gly Leu Ile Ala Ile Ile Met Val Thr Ile Met Leu Cys Cys Met Thr
1220 1225 1230
Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys
1235 1240 1245
Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu Lys Gly Val Lys Leu
1250 1255 1260
His Tyr Thr
1265
<210> 26
<211> 3804
<212> DNA
<213> Pangolin CoV GX
<400> 26
atgtttgtgt tcctattcgt cctacctctt gtgtcatcac aatgcgtgaa ccttacaaca 60
agaacaggaa tccctcctgg atacacaaac tcatcaacaa gaggagtgta ctaccctgac 120
aaggtgttta gatcatcaat ccttcacctt acacaagatc tctttctacc gttcttctcg 180
aacgtgacat ggtttaacac aatcaactac caaggaggat ttaagaagtt cgacaaccct 240
gtgcttcctt tcaatgacgg agtgtacttt gcatcaacag agaagtctaa tatcatcaga 300
ggatggatct ttggaacaac acttgatgca agaacacaat cacttcttat tgtcaataat 360
gctacgaacg tggtgatcaa agtgtgcgaa tttcaattct gtactgatcc tttcctaggc 420
gtttactacc acaacaacaa caagacctgg gtggagaatg agtttcgtgt atatagctcg 480
gcgaacaact gcacatttga atacatctca caacctttct taatggatct tgaaggaaag 540
cagggtaact ttaaaaacct tcgtgaattt gtgtttaaga atgtcgatgg atactttaag 600
atatattcaa agcatactcc aatcgacttg gttcgggatc ttcctagagg atttgcagca 660
cttgaacctc ttgtggatct tcctatcgga atcaacatca caagatttca aacacttctt 720
gcacttcaca gatcatacct tacacctgga aaccttgaat ctggctggac cacgggcgca 780
gcagcatact acgtgggata ccttcaacaa agaacatttc ttctttcata caaccagaat 840
ggaacgatta cagatgcggt cgactgttca cttgatcctc tttcagaaac aaagtgtact 900
cttaaatcac ttacagtgga gaagggtatt taccaaacat caaactttag agtgcaacct 960
acaatctcaa tcgtgagatt tcctaacatc acaaaccttt gccctttcgg tgaagtcttt 1020
aacgcatcaa agttcgcgtc agtgtacgca tggaacagaa agcgcatttc aaactgcgtg 1080
gcagattact cagtgcttta caactcaaca tcattcagca cctttaaatg ctacggagtg 1140
tcacctacaa agttaaatga tctttgcttt acaaacgtgt acgcagattc atttgtggtg 1200
aaaggagatg aagtgagaca aatcgcacct ggacaaacag gagtgatcgc agattacaac 1260
tacaaacttc ctgatgattt cactgggtgc gtgatcgcat ggaactcagt gaaacaagat 1320
gccctgactg gtggcaacta tggttattta tatcgcctct ttcggaagag taagctcaaa 1380
cctttcgagc gggacataag caccgagatc taccaagcag gatcaacacc ttgcaacgga 1440
caagtgggac ttaactgcta ctaccctctt gaaagatacg gatttcaccc tacaacagga 1500
gtgaactacc aacctttccg tgtcgtggtg ctttcatttg aacttcttaa cggacctgca 1560
acagtgtgcg gacctaaact ttcaacaacg ctcgttaagg acaagtgcgt gaactttaac 1620
ttcaatggtt tgacgggtac tggagtgctt acaacatcaa agaagcagtt tctcccattc 1680
cagcaatttg gacgcgatat ctcagatacg acggacgccg ttcgagaccc tcaaacactt 1740
gaaatccttg atatcacacc ttgctcattt ggaggagtgt cagtgatcac acctggaaca 1800
aacacatcaa accaagtggc agtgctttac caagatgtga actgcacaga agtgcctatg 1860
gcaatccacg cagaacaact tacacctgca tggcgcgtat attcggctgg tgcaaacgtg 1920
tttcaaacaa gagcaggatg ccttgtggga gcagaacacg tgaacaactc atacgaatgc 1980
gatatccctg tgggagcagg aatctgcgca tcataccact caatgtcatc acttagatca 2040
gtgaaccaaa gatcaatcat cgcatacaca atgtcacttg gagcagaaaa ctcagtggca 2100
tactcaaaca actcaatcgc aatccctaca aactttacaa tctcagtgac aacagagatt 2160
ctcccagtgt caatgacaaa gacttccgtg gattgcacaa tgtacatctg cggagattca 2220
atcgaatgct caaaccttct tcttcaatac ggatcattct gtactcaact taacagagcc 2280
ctaacgggca tagctgtgga acaagataag aatacgcaag aagtgtttgc acaagtgaaa 2340
caaatctaca agactccgcc tatcaaagat ttcggcggtt tcaatttcag tcagatatta 2400
ccagacccta gcaaaccgag caagcgctca tttatcgaag atctgctctt taataaggtg 2460
acacttgcag atgcaggatt tatcaaacaa tacggagatt gcctcggcga catagccgcc 2520
agagatctta tctgcgcaca gaagtttaac ggcctgaccg tattgcctcc tcttcttaca 2580
gatgaaatga tcgcacaata cacatcagca cttcttgcag ggacgataac tagcggatgg 2640
actttcggtg cgggcgcagc acttcaaatc cctttcgcga tgcaaatggc atacagattt 2700
aacggaatcg gagtgacaca gaatgtactt tacgagaatc agaaactcat tgcaaaccaa 2760
tttaactcag caatcggaaa gattcaggat tcactttcat caacagcatc agcacttgga 2820
aagctccagg atgtggtgaa ccagaatgcg caagcactta acacgttggt taagcagcta 2880
tcatcaaact ttggagcaat ctcatcagtg cttaacgata tcctttcaag acttgataaa 2940
gtggaagcag aagtgcagat tgaccgtcta attacgggaa gacttcaatc acttcaaaca 3000
tacgttaccc agcagttaat aagagcagca gaaatcagag catcagcaaa ccttgcagca 3060
acaaagatgt cggaatgcgt gcttggacaa tcaaagaggg tagatttctg tggcaagggc 3120
taccatctta tgtcatttcc tcaatcagca cctcacggag tggtgtttct tcacgtgaca 3180
tacgtgcctg cacaagagaa gaatttcaca acagcacctg caatctgcca cgaaggaaag 3240
gctcatttcc cgcgagaagg agtgtttgtg tcaaacggaa cacactggtt tatcacacaa 3300
agaaacttct atgagcctca aatcatcaca acagataaca catttgtgtc aggatcatgc 3360
gatgtggtga tcggaatagt taacaacaca gtatatgacc ctcttcaacc tgaacttgat 3420
tcatttaaag aagaacttga taaatacttt aaaaaccaca catcacctga tgtggatttg 3480
ggagatattt cagggataaa cgcatcagtg gtgaacatcc agaaggaaat cgaccgactc 3540
aatgaggtgg caaagaatct aaacgaaagc ccgattgacc tccaggagct tggaaagtat 3600
gagcaataca tcaaatggcc ttggtacatc tggcttggat ttatcgcagg acttatcgca 3660
atcatcatgg tgacaatcat gctttgctgc atgacatcat gttgttcgtg tctcaaggga 3720
tgttgttctt gtggtagttg ctgcaaattt gatgaagatg attcagaacc tgtgcttaaa 3780
ggagtgaaac ttcactacac atga 3804
<210> 27
<211> 1255
<212> PRT
<213> Bat CoV
<400> 27
Met Phe Ile Phe Leu Phe Phe Leu Thr Leu Thr Ser Gly Ser Asp Leu
1 5 10 15
Glu Ser Cys Thr Thr Phe Asp Asp Val Gln Ala Pro Asn Tyr Pro Gln
20 25 30
His Ser Ser Ser Arg Arg Gly Val Tyr Tyr Pro Asp Glu Ile Phe Arg
35 40 45
Ser Asp Thr Leu Tyr Leu Thr Gln Asp Leu Phe Leu Pro Phe Tyr Ser
50 55 60
Asn Val Thr Gly Phe His Thr Ile Asn His Arg Phe Asp Asn Pro Val
65 70 75 80
Ile Pro Phe Lys Asp Gly Val Tyr Phe Ala Ala Thr Glu Lys Ser Asn
85 90 95
Val Val Arg Gly Trp Val Phe Gly Ser Thr Met Asn Asn Lys Ser Gln
100 105 110
Ser Val Ile Ile Ile Asn Asn Ser Thr Asn Val Val Ile Arg Ala Cys
115 120 125
Asn Phe Glu Leu Cys Asp Asn Pro Phe Phe Ala Val Ser Lys Pro Thr
130 135 140
Gly Thr Gln Thr His Thr Met Ile Phe Asp Asn Ala Phe Asn Cys Thr
145 150 155 160
Phe Glu Tyr Ile Ser Asp Ser Phe Ser Leu Asp Val Ala Glu Lys Ser
165 170 175
Gly Asn Phe Lys His Leu Arg Glu Phe Val Phe Lys Asn Lys Asp Gly
180 185 190
Phe Leu Tyr Val Tyr Lys Gly Tyr Gln Pro Ile Asp Val Val Arg Asp
195 200 205
Leu Pro Ser Gly Phe Asn Ile Leu Lys Pro Ile Phe Lys Leu Pro Leu
210 215 220
Gly Ile Asn Ile Thr Asn Phe Arg Ala Ile Leu Thr Ala Phe Leu Pro
225 230 235 240
Ala Gln Asp Thr Trp Gly Thr Ser Ala Ala Ala Tyr Phe Val Gly Tyr
245 250 255
Leu Lys Pro Ala Thr Phe Met Leu Lys Tyr Asp Glu Asn Gly Thr Ile
260 265 270
Thr Asp Ala Val Asp Cys Ser Gln Asn Pro Leu Ala Glu Leu Lys Cys
275 280 285
Ser Val Lys Ser Phe Glu Ile Asp Lys Gly Ile Tyr Gln Thr Ser Asn
290 295 300
Phe Arg Val Ala Pro Ser Lys Glu Val Val Arg Phe Pro Asn Ile Thr
305 310 315 320
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Thr Phe Pro Ser
325 330 335
Val Tyr Ala Trp Glu Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr
340 345 350
Ser Val Leu Tyr Asn Ser Thr Ser Phe Ser Thr Phe Lys Cys Tyr Gly
355 360 365
Val Ser Ala Thr Lys Leu Asn Asp Leu Cys Phe Ser Asn Val Tyr Ala
370 375 380
Asp Ser Phe Val Val Lys Gly Asp Asp Val Arg Gln Ile Ala Pro Gly
385 390 395 400
Gln Thr Gly Val Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe
405 410 415
Thr Gly Cys Val Leu Ala Trp Asn Thr Arg Asn Ile Asp Ala Thr Gln
420 425 430
Thr Gly Asn Tyr Asn Tyr Lys Tyr Arg Ser Leu Arg His Gly Lys Leu
435 440 445
Arg Pro Phe Glu Arg Asp Ile Ser Asn Val Pro Phe Ser Pro Asp Gly
450 455 460
Lys Pro Cys Thr Pro Pro Ala Phe Asn Cys Tyr Trp Pro Leu Asn Asp
465 470 475 480
Tyr Gly Phe Tyr Ile Thr Asn Gly Ile Gly Tyr Gln Pro Tyr Arg Val
485 490 495
Val Val Leu Ser Phe Glu Leu Leu Asn Ala Pro Ala Thr Val Cys Gly
500 505 510
Pro Lys Leu Ser Thr Asp Leu Ile Lys Asn Gln Cys Val Asn Phe Asn
515 520 525
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Pro Ser Ser Lys Arg
530 535 540
Phe Gln Pro Phe Gln Gln Phe Gly Arg Asp Val Leu Asp Phe Thr Asp
545 550 555 560
Ser Val Arg Asp Pro Lys Thr Ser Glu Ile Leu Asp Ile Ser Pro Cys
565 570 575
Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Ser
580 585 590
Glu Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Asp Val Pro Val
595 600 605
Ala Ile His Ala Asp Gln Leu Thr Pro Ser Trp Arg Val Tyr Ser Thr
610 615 620
Gly Asn Asn Val Phe Gln Thr Gln Ala Gly Cys Leu Ile Gly Ala Glu
625 630 635 640
His Val Asp Thr Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile
645 650 655
Cys Ala Ser Tyr His Thr Val Ser Ser Leu Arg Ser Thr Ser Gln Lys
660 665 670
Ser Ile Val Ala Tyr Thr Met Ser Leu Gly Ala Asp Ser Ser Ile Ala
675 680 685
Tyr Ser Asn Asn Thr Ile Ala Ile Pro Thr Asn Phe Ser Ile Ser Ile
690 695 700
Thr Thr Glu Val Met Pro Val Ser Met Ala Lys Thr Ser Val Asp Cys
705 710 715 720
Asn Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ala Asn Leu Leu Leu
725 730 735
Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Ser Gly Ile
740 745 750
Ala Val Glu Gln Asp Arg Asn Thr Arg Glu Val Phe Ala Gln Val Lys
755 760 765
Gln Met Tyr Lys Thr Pro Thr Leu Lys Asp Phe Gly Gly Phe Asn Phe
770 775 780
Ser Gln Ile Leu Pro Asp Pro Leu Lys Pro Thr Lys Arg Ser Phe Ile
785 790 795 800
Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Met
805 810 815
Lys Gln Tyr Gly Glu Cys Leu Gly Asp Ile Asn Ala Arg Asp Leu Ile
820 825 830
Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr
835 840 845
Asp Asp Met Ile Ala Ala Tyr Thr Ala Ala Leu Val Ser Gly Thr Ala
850 855 860
Thr Ala Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe
865 870 875 880
Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn
885 890 895
Val Leu Tyr Glu Asn Gln Lys Gln Ile Ala Asn Gln Phe Asn Lys Ala
900 905 910
Ile Ser Gln Ile Gln Glu Ser Leu Thr Thr Thr Ser Thr Ala Leu Gly
915 920 925
Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu
930 935 940
Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn
945 950 955 960
Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp
965 970 975
Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln
980 985 990
Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala
995 1000 1005
Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe
1010 1015 1020
Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ala Ala Pro His
1025 1030 1035 1040
Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ser Gln Glu Arg Asn
1045 1050 1055
Phe Thr Thr Ala Pro Ala Ile Cys His Glu Gly Lys Ala Tyr Phe Pro
1060 1065 1070
Arg Glu Gly Val Phe Val Phe Asn Gly Thr Ser Trp Phe Ile Thr Gln
1075 1080 1085
Arg Asn Phe Phe Ser Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val
1090 1095 1100
Ser Gly Ser Cys Asp Val Val Ile Gly Ile Ile Asn Asn Thr Val Tyr
1105 1110 1115 1120
Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys
1125 1130 1135
Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser
1140 1145 1150
Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu
1155 1160 1165
Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu
1170 1175 1180
Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Val Trp Leu
1185 1190 1195 1200
Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Leu Leu
1205 1210 1215
Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Ala Cys Ser Cys
1220 1225 1230
Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu Lys
1235 1240 1245
Gly Val Lys Leu His Tyr Thr
1250 1255
<210> 28
<211> 3768
<212> DNA
<213> Bat CoV
<400> 28
atgttcatct ttctgttctt cctcactctg actagcggtt ccgatctgga gagctgcact 60
acctttgacg acgtccaagc tcccaattac ccccaacact ccagctcccg tcgtggtgtc 120
tattaccccg acgaaatctt ccgctccgac actctgtatc tgactcaaga cctcttcctc 180
cctttttaca gcaacgtcac tggcttccac accatcaatc accgctttga caaccccgtg 240
atccccttca aggacggcgt gtacttcgct gccaccgaaa aatccaacgt cgtgcgcggc 300
tgggtcttcg gctccaccat gaacaacaag tcccaatccg tcattatcat caacaactcc 360
actaacgtcg tcatccgcgc ttgtaacttc gagctgtgcg ataacccctt cttcgctgtc 420
agcaaaccta ctggtaccca aactcatacc atgatcttcg acaacgcttt taactgtact 480
ttcgagtata tcagcgacag cttctctctg gacgtggccg agaagagcgg taattttaag 540
catctgcgcg agttcgtctt taagaataag gatggctttc tgtacgtgta caagggttat 600
caacccatcg acgtcgtccg tgacctcccc agcggcttca atattctcaa acccatcttt 660
aagctgcctc tgggcatcaa catcaccaac ttccgtgcca tcctcactgc ctttctgccc 720
gctcaagata cttggggcac ctccgccgct gcttacttcg tgggttatct gaaacccgcc 780
acttttatgc tgaagtacga tgagaatggt actatcactg acgccgtgga ctgttcccag 840
aaccccctcg ctgagctcaa gtgcagcgtc aagtccttcg agatcgacaa gggcatctac 900
caaacctcca atttccgtgt cgctccttcc aaagaggtgg tgcgcttccc caacatcact 960
aacctctgtc cctttggcga agtcttcaat gccaccacct ttccttccgt ctacgcttgg 1020
gagcgcaagc gtatttccaa ctgcgtcgct gactactccg tgctgtacaa tagcacttcc 1080
ttcagcactt ttaagtgtta cggcgtgtcc gctactaagc tcaacgatct gtgcttttcc 1140
aacgtgtacg ccgactcctt cgtggtgaag ggtgatgacg tccgtcagat cgctcccggc 1200
caaactggtg tgattgctga ctacaattac aagctgcccg acgatttcac cggctgtgtc 1260
ctcgcttgga acacccgtaa catcgacgct acccagaccg gcaactacaa ctacaagtat 1320
cgctctctgc gccatggtaa actccgtccc ttcgagcgcg atattagcaa cgtccctttt 1380
agccccgatg gtaagccttg cacccctccc gctttcaact gctattggcc tctgaacgac 1440
tacggtttct atatcactaa cggcatcggt taccagccct accgtgtcgt cgtgctgagc 1500
tttgaactgc tgaatgcccc cgctaccgtg tgcggcccta agctctccac cgatctgatc 1560
aagaaccagt gcgtgaattt taactttaat ggtctgactg gcaccggcgt gctgactcct 1620
agctccaagc gcttccagcc ctttcagcaa ttcggccgtg acgtgctgga cttcactgac 1680
tccgtgcgcg accccaagac ttccgaaatt ctggacatct ccccttgctc ctttggcggc 1740
gtctccgtca ttactcccgg taccaatact agcagcgaag tggctgtgct gtaccaagac 1800
gtgaactgca ctgacgtgcc cgtcgctatt cacgccgacc aactcactcc cagctggcgt 1860
gtgtatagca ctggcaacaa cgtcttccag acccaagctg gctgtctgat tggcgctgaa 1920
catgtcgaca cttcctacga gtgcgacatc cccatcggcg ctggtatctg tgcctcctat 1980
cacaccgtct ccagcctccg ctccaccagc cagaagtcca tcgtggctta cactatgtcc 2040
ctcggcgctg actccagcat cgcctactcc aacaacacta tcgccatccc caccaacttc 2100
tccatttcca tcactaccga agtcatgccc gtgagcatgg ccaagacctc cgtggactgt 2160
aacatgtata tctgtggtga ctccaccgag tgcgctaatc tgctgctgca gtatggttcc 2220
ttctgtaccc agctcaaccg cgccctcagc ggcattgctg tcgaacaaga ccgcaatacc 2280
cgtgaagtgt tcgcccaagt gaaacagatg tacaagaccc ctaccctcaa ggactttggc 2340
ggcttcaact tctcccagat tctgcccgac cctctgaagc ctaccaagcg ttccttcatc 2400
gaggatctgc tgttcaataa ggtcactctg gctgacgctg gctttatgaa gcagtatggc 2460
gagtgtctgg gcgacattaa cgcccgcgat ctcatttgcg ctcagaagtt caacggtctc 2520
accgtgctcc ctcctctgct caccgatgac atgatcgccg cttacactgc tgctctggtg 2580
agcggtactg ctaccgctgg ctggactttc ggcgctggtg ctgctctgca aatccctttt 2640
gccatgcaga tggcctaccg cttcaacggt atcggcgtca cccagaacgt cctctacgag 2700
aatcagaagc agattgctaa ccaatttaac aaagctattt cccaaatcca agagtccctc 2760
actaccacta gcactgccct cggcaagctc caagacgtgg tgaaccagaa cgcccaagcc 2820
ctcaacactc tggtgaagca actgagcagc aatttcggcg ccattagcag cgtgctcaat 2880
gacattctgt cccgcctcga caaagtcgag gccgaggtgc agattgaccg tctgatcact 2940
ggtcgtctgc aatccctcca gacctacgtc actcagcagc tgatccgtgc cgctgagatt 3000
cgcgcctccg ccaatctggc cgctactaaa atgagcgagt gcgtcctcgg ccaatccaag 3060
cgcgtcgact tctgtggcaa gggctatcat ctgatgagct ttcctcaagc tgccccccat 3120
ggcgtggtgt ttctgcacgt gacctatgtc ccctcccaag aacgcaactt cactaccgct 3180
cccgctatct gtcacgaggg taaggcttat tttccccgtg agggcgtgtt cgtgttcaat 3240
ggcaccagct ggttcatcac ccaacgtaac ttcttctccc ctcagattat caccaccgac 3300
aatacttttg tctccggcag ctgcgatgtc gtgatcggca tcatcaataa tactgtctat 3360
gatcctctgc agcccgagct cgattccttc aaggaggagc tcgataaata cttcaagaac 3420
cacacctccc ccgacgtcga cctcggtgat attagcggca tcaacgctag cgtggtgaac 3480
atccagaaag agatcgaccg cctcaacgag gtggccaaaa atctgaatga gtctctgatc 3540
gatctgcaag agctcggtaa gtacgagcag tacatcaagt ggccttggta cgtgtggctg 3600
ggcttcatcg ccggactgat cgccatcgtg atggtgacca ttctgctgtg ctgcatgacc 3660
agctgctgca gctgtctgaa gggcgcttgc agctgcggca gctgctgcaa gttcgacgag 3720
gacgacagcg agcccgtgct gaagggcgtg aagctgcact acacatga 3768
<210> 29
<211> 1278
<212> PRT
<213> Bat CoV
<400> 29
Met Phe Leu Leu Thr Thr Lys Arg Thr Met Phe Val Phe Leu Val Leu
1 5 10 15
Leu Pro Leu Val Ser Ser Gln Cys Val Asn Leu Thr Thr Arg Thr Gln
20 25 30
Leu Pro Pro Ala Tyr Thr Asn Ser Ser Thr Arg Gly Val Tyr Tyr Pro
35 40 45
Asp Lys Val Phe Arg Ser Ser Val Leu His Leu Thr Gln Asp Leu Phe
50 55 60
Leu Pro Phe Phe Ser Asn Val Thr Trp Phe His Ala Ile His Val Ser
65 70 75 80
Gly Thr Asn Gly Ile Lys Arg Phe Asp Asn Pro Val Leu Pro Phe Asn
85 90 95
Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly
100 105 110
Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile
115 120 125
Val Asn Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu Phe Gln Phe
130 135 140
Cys Asn Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn Asn Lys Ser
145 150 155 160
Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr
165 170 175
Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu Gly Lys Gln
180 185 190
Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn Ile Asp Gly
195 200 205
Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu Val Arg Asp
210 215 220
Leu Pro Pro Gly Phe Ser Ala Leu Glu Pro Leu Val Asp Leu Pro Ile
225 230 235 240
Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser
245 250 255
Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala
260 265 270
Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr
275 280 285
Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro
290 295 300
Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly
305 310 315 320
Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Asp Ser Ile Val
325 330 335
Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn
340 345 350
Ala Thr Thr Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser
355 360 365
Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Thr Ser Phe Ser
370 375 380
Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys
385 390 395 400
Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Thr Gly Asp Glu Val
405 410 415
Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr
420 425 430
Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Lys
435 440 445
His Ile Asp Ala Lys Glu Gly Gly Asn Phe Asn Tyr Leu Tyr Arg Leu
450 455 460
Phe Arg Lys Ala Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu
465 470 475 480
Ile Tyr Gln Ala Gly Ser Lys Pro Cys Asn Gly Gln Thr Gly Leu Asn
485 490 495
Cys Tyr Tyr Pro Leu Tyr Arg Tyr Gly Phe Tyr Pro Thr Asp Gly Val
500 505 510
Gly His Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu Asn
515 520 525
Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys
530 535 540
Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val
545 550 555 560
Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg
565 570 575
Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu
580 585 590
Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr
595 600 605
Pro Gly Thr Asn Ala Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val
610 615 620
Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro
625 630 635 640
Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala
645 650 655
Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp
660 665 670
Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn
675 680 685
Ser Arg Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu
690 695 700
Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro
705 710 715 720
Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met
725 730 735
Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr
740 745 750
Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu
755 760 765
Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln
770 775 780
Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys
785 790 795 800
Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys
805 810 815
Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr
820 825 830
Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp
835 840 845
Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr
850 855 860
Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser
865 870 875 880
Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly
885 890 895
Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn
900 905 910
Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile
915 920 925
Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser
930 935 940
Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn
945 950 955 960
Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly
965 970 975
Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val
980 985 990
Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser
995 1000 1005
Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg
1010 1015 1020
Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly
1025 1030 1035 1040
Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser
1045 1050 1055
Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr
1060 1065 1070
Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His
1075 1080 1085
Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly
1090 1095 1100
Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile
1105 1110 1115 1120
Thr Thr Asp Asn Thr Phe Val Ser Gly Ser Cys Asp Val Val Ile Gly
1125 1130 1135
Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser
1140 1145 1150
Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp
1155 1160 1165
Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile
1170 1175 1180
Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu
1185 1190 1195 1200
Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys
1205 1210 1215
Trp Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile
1220 1225 1230
Ile Met Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys
1235 1240 1245
Leu Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp
1250 1255 1260
Asp Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270 1275
<210> 30
<211> 3837
<212> DNA
<213> Bat CoV
<400> 30
atgttcctgc tgaccaccaa gcgcaccatg ttcgtgttcc tggtgctgct gcccctggtg 60
agcagccagt gcgtgaacct gaccacccgc acccagctgc cccccgccta caccaacagc 120
agcacccgcg gcgtgtacta ccccgacaag gtgttccgca gcagcgtgct gcacctgacc 180
caggacctgt tcctgccctt cttcagcaac gtgacctggt tccacgccat ccacgtgagc 240
ggcaccaacg gcatcaagcg cttcgacaac cccgtgctgc ccttcaacga cggcgtgtac 300
ttcgccagca ccgagaagag caacatcatc cgcggctgga tcttcggcac caccctggac 360
agcaagaccc agagcctgct gatcgtgaac aacgccacca acgtggtgat caaggtgtgc 420
gagttccagt tctgcaacga ccccttcctg ggcgtgtact accacaagaa caacaagagc 480
tggatggaga gcgagttccg cgtgtacagc agcgccaaca actgcacctt cgagtacgtg 540
agccagccct tcctgatgga cctggagggc aagcagggca acttcaagaa cctgcgcgag 600
ttcgtgttca agaacatcga cggctacttc aagatctaca gcaagcacac ccccatcaac 660
ctggtgcgcg acctgccccc cggcttcagc gccctggagc ccctggtgga cctgcccatc 720
ggcatcaaca tcacccgctt ccagaccctg ctggccctgc accgcagcta cctgaccccc 780
ggcgacagca gcagcggctg gaccgccggc gccgccgcct actacgtggg ctacctgcag 840
ccccgcacct tcctgctgaa gtacaacgag aacggcacca tcaccgacgc cgtggactgc 900
gccctggacc ccctgagcga gaccaagtgc accctgaaga gcttcaccgt ggagaagggc 960
atctaccaga ccagcaactt ccgcgtgcag cccaccgaca gcatcgtgcg cttccccaac 1020
atcaccaacc tgtgcccctt cggcgaggtg ttcaacgcca ccaccttcgc cagcgtgtac 1080
gcctggaacc gcaagcgcat cagcaactgc gtggccgact acagcgtgct gtacaacagc 1140
accagcttca gcaccttcaa gtgctacggc gtgagcccca ccaagctgaa cgacctgtgc 1200
ttcaccaacg tgtacgccga cagcttcgtg atcaccggcg acgaggtgcg ccagatcgcc 1260
cccggccaga ccggcaagat cgccgactac aactacaagc tgcccgacga cttcaccggc 1320
tgcgtgatcg cctggaacag caagcacatc gacgccaagg agggcggcaa cttcaactac 1380
ctgtaccgcc tgttccgcaa ggccaacctg aagcccttcg agcgcgacat cagcaccgag 1440
atctaccagg ccggcagcaa gccctgcaac ggccagaccg gcctgaactg ctactacccc 1500
ctgtaccgct acggcttcta ccccaccgac ggcgtgggcc accagcccta ccgcgtggtg 1560
gtgctgagct tcgagctgct gaacgccccc gccaccgtgt gcggccccaa gaagagcacc 1620
aacctggtga agaacaagtg cgtgaacttc aacttcaacg gcctgaccgg caccggcgtg 1680
ctgaccgaga gcaacaagaa gttcctgccc ttccagcagt tcggccgcga catcgccgac 1740
accaccgacg ccgtgcgcga cccccagacc ctggagatcc tggacatcac cccctgcagc 1800
ttcggcggcg tgagcgtgat cacccccggc accaacgcca gcaaccaggt ggccgtgctg 1860
taccaggacg tgaactgcac cgaggtgccc gtggccatcc acgccgacca gctgaccccc 1920
acctggcgcg tgtacagcac cggcagcaac gtgttccaga cccgcgccgg ctgcctgatc 1980
ggcgccgagc acgtgaacaa cagctacgag tgcgacatcc ccatcggcgc cggcatctgc 2040
gccagctacc agacccagac caacagccgc agcgtggcca gccagagcat catcgcctac 2100
accatgagcc tgggcgccga gaacagcgtg gcctacagca acaacagcat cgccatcccc 2160
accaacttca ccatcagcgt gaccaccgag atcctgcccg tgagcatgac caagaccagc 2220
gtggactgca ccatgtacat ctgcggcgac agcaccgagt gcagcaacct gctgctgcag 2280
tacggcagct tctgcaccca gctgaaccgc gccctgaccg gcatcgccgt ggagcaggac 2340
aagaacaccc aggaggtgtt cgcccaggtg aagcagatct acaagacccc ccccatcaag 2400
gacttcggcg gcttcaactt cagccagatc ctgcccgacc ccagcaagcc cagcaagcgc 2460
agcttcatcg aggacctgct gttcaacaag gtgaccctgg ccgacgccgg cttcatcaag 2520
cagtacggcg actgcctggg cgacatcgcc gcccgcgacc tgatctgcgc ccagaagttc 2580
aacggcctga ccgtgctgcc ccccctgctg accgacgaga tgatcgccca gtacaccagc 2640
gccctgctgg ccggcaccat caccagcggc tggaccttcg gcgccggcgc cgccctgcag 2700
atccccttcg ccatgcagat ggcctaccgc ttcaacggca tcggcgtgac ccagaacgtg 2760
ctgtacgaga accagaagct gatcgccaac cagttcaaca gcgccatcgg caagatccag 2820
gacagcctga gcagcaccgc cagcgccctg ggcaagctgc aggacgtggt gaaccagaac 2880
gcccaggccc tgaacaccct ggtgaagcag ctgagcagca acttcggcgc catcagcagc 2940
gtgctgaacg acatcctgag ccgcctggac aaggtggagg ccgaggtgca gatcgaccgc 3000
ctgatcaccg gccgcctgca gagcctgcag acctacgtga cccagcagct gatccgcgcc 3060
gccgagatcc gcgccagcgc caacctggcc gccaccaaga tgagcgagtg cgtgctgggc 3120
cagagcaagc gcgtggactt ctgcggcaag ggctaccacc tgatgagctt cccccagagc 3180
gccccccacg gcgtggtgtt cctgcacgtg acctacgtgc ccgcccagga gaagaacttc 3240
accaccgccc ccgccatctg ccacgacggc aaggcccact tcccccgcga gggcgtgttc 3300
gtgagcaacg gcacccactg gttcgtgacc cagcgcaact tctacgagcc ccagatcatc 3360
accaccgaca acaccttcgt gagcggcagc tgcgacgtgg tgatcggcat cgtgaacaac 3420
accgtgtacg accccctgca gcccgagctg gacagcttca aggaggagct ggacaagtac 3480
ttcaagaacc acaccagccc cgacgtggac ctgggcgaca tcagcggcat caacgccagc 3540
gtggtgaaca tccagaagga gatcgaccgc ctgaacgagg tggccaagaa cctgaacgag 3600
agcctgatcg acctgcagga gctgggcaag tacgagcagt acatcaagtg gccctggtac 3660
atctggctgg gcttcatcgc cggcctgatc gccatcatca tggtgaccat catgctgtgc 3720
tgcatgacca gctgctgcag ctgcctgaag ggctgctgca gctgcggcag ctgctgcaag 3780
ttcgacgagg acgacagcga gcccgtgctg aagggcgtga agctgcacta cacctga 3837
<210> 31
<211> 107
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
atgggatggt catgtatcat cctttttcta gtagcaactg caacctgtgt acattcagcg 60
gccgcggagg tggaggtagt gctagccatc accatcacca tcactaa 107
<210> 32
<211> 232
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 32
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala
1 5 10 15
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro
20 25 30
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
35 40 45
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val
50 55 60
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
65 70 75 80
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln
85 90 95
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
100 105 110
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
115 120 125
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr
130 135 140
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
145 150 155 160
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
165 170 175
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
180 185 190
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe
195 200 205
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys
210 215 220
Ser Leu Ser Leu Ser Pro Gly Lys
225 230

Claims (10)

1.4种抗体中的任意一种或任意组合；
所述4种抗体为：TH-34抗体、TH-41抗体、TH-44抗体和TH-40抗体；
所述TH-34抗体包括框架区FR和互补决定区CDR；所述TH-34抗体中的CDR1、CDR2和CDR3依次为序列表的序列8中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-34抗体中的FR1、FR2、FR3和FR4依次为序列表的序列8中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基；
所述TH-41抗体包括框架区FR和互补决定区CDR；所述TH-41抗体中的CDR1、CDR2和CDR3依次为序列表的序列10中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-41抗体中的FR1、FR2、FR3和FR4依次为序列表的序列10中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基；
所述TH-44抗体包括框架区FR和互补决定区CDR；所述TH-44抗体中的CDR1、CDR2和CDR3依次为序列表的序列12中第26-33位、第51-57位、第96-105位氨基酸残基；所述TH-44抗体中的FR1、FR2、FR3和FR4依次为序列表的序列12中第1-25位、第34-50位、第58-95位、第105-116位氨基酸残基；
所述TH-40抗体包括框架区FR和互补决定区CDR；所述TH-40抗体中的CDR1、CDR2和CDR3依次为序列表的序列14中第26-33位、第51-58位、第97-115位氨基酸残基；所述TH-40抗体中的FR1、FR2、FR3和FR4依次为序列表的序列14中第1-25位、第34-50位、第59-96位、第116-126位氨基酸残基。
2.4种抗体中的任意一种或任意组合；
所述4种抗体为：TH-34-Fc抗体、TH-41-Fc抗体、TH-44-Fc抗体和TH-40-Fc抗体；
所述TH-34-Fc抗体自N端至C端包括如下区段：TH-34抗体、Fc；
所述TH-41-Fc抗体自N端至C端包括如下区段：TH-41抗体、Fc；
所述TH-44-Fc抗体自N端至C端包括如下区段：TH-44抗体、Fc；
所述TH-40-Fc抗体自N端至C端包括如下区段：TH-40抗体、Fc；
TH-34抗体为权利要求1中所述的TH-34抗体；TH-41抗体为权利要求1中所述的TH-41抗体；TH-44抗体为权利要求1中所述的TH-44抗体；TH-40抗体为权利要求1中所述的TH-40抗体。
3.4种基因中的任意一种或任意组合；
所述4种基因为：编码TH-34抗体的基因、编码TH-41抗体的基因、编码TH-44抗体的基因、编码TH-40抗体的基因；
TH-34抗体为权利要求1中所述的TH-34抗体；TH-41抗体为权利要求1中所述的TH-41抗体；TH-44抗体为权利要求1中所述的TH-44抗体；TH-40抗体为权利要求1中所述的TH-40抗体。
4.4种基因中的任意一种或任意组合；
所述4种基因为：编码TH-34-Fc抗体的基因、编码TH-41-Fc抗体的基因、编码TH-44-Fc抗体的基因、编码TH-40-Fc抗体的基因；
TH-34-Fc抗体为权利要求2所述的TH-34-Fc抗体；TH-41-Fc抗体为权利要求2所述的TH-41-Fc抗体；TH-44-Fc抗体为权利要求2所述的TH-44-Fc抗体；TH-40-Fc抗体为权利要求2所述的TH-40-Fc抗体。
5.权利要求1或2所述抗体或抗体组合在制备用于抑制冠状病毒的药物中的应用。
6.一种用于抑制冠状病毒的药物，其活性成分为权利要求1或2所述抗体或抗体组合。
7.权利要求1或2所述抗体或抗体组合在制备用于中和冠状病毒的药物中的应用。
8.一种用于中和冠状病毒的药物，其活性成分为权利要求1或2所述抗体或抗体组合。
9.权利要求1或2所述抗体或抗体组合在制备用于预防和/或治疗冠状病毒引起的疾病的药物中的应用。
10.一种用于预防和/或治疗冠状病毒引起的疾病的药物，其活性成分为权利要求1或2所述抗体或抗体组合。
CN202210724658.1A 2022-06-24 2022-06-24 广谱中和SARS-CoV-2以及其他沙贝病毒的抗体及其应用 Pending CN117327173A (zh)

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