KR101848943B1 - 생물학적 입자의 클러스터를 검출하는 방법 - Google Patents
생물학적 입자의 클러스터를 검출하는 방법 Download PDFInfo
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Abstract
Description
도 1은 박테리아의 콜로니를 나타낸 배지의 표면을 도시한다.
도 2는 본 발명의 구현에서 방법의 다양한 단계를 나타내는 도면이다.
도 3은 본 발명의 방법의 제 1 변형에서 이용되는 크로마틱 공초점 미세지형의 원리를 예시한다.
도 4a, 도 4b 및 도 5A, 도 5B, 도 5C, 도 5D 및 도 5E는 박테리아로 시드된 배지에 크로마틱 공초점 미세지형 기술을 적용함으로써 획득되는 실험 결과를 도시한다.
도 6a 내지 도 6d는 슐리렌 사진의 기술에 기초하여 본 발명의 방법의 제 2 변형을 도시한다.
도 7a 내지 도 7c는 옴브로스카피의 기술에 기초하여 본 발명의 방법의 제 3 변형을 도시한다.
Claims (12)
- 표면(11) 상에서 생물학적 입자(12)의 클러스터를 검출하는 방법으로서,
a. 3차원 표현을 얻을 수 있는 미세지형 광 기술을 이용하여 상기 표면(11)의 3차원 지형 표현(20)을 결정하는 단계(E1);
b. 상기 3차원 지형 표현(20)을 전처리하는 동작을 수행하되, 상기 전처리 동작은 차단 주파수를 이용하는 로우패스 공간 필터링을 통해 베이스 표면을 검출하고 삭감하는 단계(E2);
c. 상기 3 차원 지형 표현(20)에서 동일 경사 또는 그레이 레벨을 이용하여 적어도 하나의 윤곽(22)를 결정하는 단계(E3); 및
d. 상기 적어도 하나의 윤곽(22)을 이용하여 생물학적 입자의 클러스터에 잠재적으로 대응하는 폐쇄 영역(23)을 특정하는 단계(E4)를 포함하고,
이 단계들은 전자 데이터 프로세서 수단의 도움으로 구현되며,
상기 3차원 지형 표현(20)은, 상기 표면(11)의 3차원 시트, 또는 2개의 인접한 픽셀 사이의 강도의 변화가 상기 표면(11)의 고도 변화를 나타내는 2차원 이미지인 것을 포함하는 것을 특징으로 하는
방법. - 제 1 항에 있어서,
상기 생물학적 입자는 박테리아, 효모, 또는 진균과 같은 미생물, 및 식물 또는 동물 세포로부터 선택되는 것을 특징으로 하는 방법. - 제 1 항 또는 제 2 항에 있어서,
상기 생물학적 입자는 100 ㎛ 이하인 직경 또는 주 치수를 나타내는 것을 특징으로 하는 방법. - 제 1 항에 있어서,
상기 표면(11)은 배지 및 공기와 같은 주위 매체 사이의 계면, 기능화 기판의 표면, 및 미소공성 막의 표면으로부터 선택되는 것을 특징으로 하는 방법. - 제 1 항에 있어서,
상기 표면의 지형 표현을 결정하는 상기 단계 a는 접촉없이 및 샘플의 준비없이 수행되는 광학 방법에 의해 구현되는 것을 특징으로 하는 방법. - 제 5 항에 있어서,
상기 표면의 지형 표현을 결정하는 상기 단계 a는 크로마틱 공초점 미세지형에 의해 구현되는 것을 특징으로 하는 방법. - 제 5 항에 있어서,
상기 표면의 구조의 지형 표현을 결정하는 상기 단계 a는 슐리렌 사진에 의해 또는 옴브로스카피에 의해 구현되는 것을 특징으로 하는 방법. - 제 1 항에 있어서,
상기 적어도 하나의 윤곽을 결정하는 상기 단계 c는 임계화에 의해 상기 표면의 상기 지형 표현의 국부 경사를 측정함으로써 구현되는 것을 특징으로 하는 방법. - 삭제
- 제 1 항에 있어서,
연속적으로 단계 a 내지 d를 반복하는 단계, 및 단계 c 또는 d에서 식별되며 시간에 따라 변화하는 형상 또는 사이즈인 그 영역을 단지 선택하는 단계를 포함하는 것을 특징으로 하는 방법. - 제 1 항에 있어서,
상기 클러스터에 존재하는 상기 생물학적 입자의 양을 평가하는 추가적인 단계 e를 더 포함하는 것을 특징으로 하는 방법. - 제 2 항에 있어서,
상기 클러스터로 배치되는 미생물의 인 시츄 또는 엑스 시츄 식별의 추가적인 단계를 포함하는 것을 특징으로 하는 방법.
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FR10/01406 | 2010-04-06 | ||
FR1001406A FR2958298B1 (fr) | 2010-04-06 | 2010-04-06 | Procede de detection d'amas de particules biologiques |
PCT/IB2011/051481 WO2011125033A1 (fr) | 2010-04-06 | 2011-04-06 | Procédé de détection d'amas de particules biologiques. |
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KR20130047691A KR20130047691A (ko) | 2013-05-08 |
KR101848943B1 true KR101848943B1 (ko) | 2018-04-13 |
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US (1) | US8855397B2 (ko) |
EP (1) | EP2556164B1 (ko) |
JP (1) | JP5907947B2 (ko) |
KR (1) | KR101848943B1 (ko) |
CN (1) | CN103025888B (ko) |
BR (1) | BR112012025587B1 (ko) |
FR (1) | FR2958298B1 (ko) |
RU (1) | RU2604794C2 (ko) |
WO (1) | WO2011125033A1 (ko) |
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AU2015212758B2 (en) | 2014-01-30 | 2019-11-21 | Bd Kiestra B.V. | A system and method for image acquisition using supervised high quality imaging |
EP3134848B1 (en) | 2014-04-22 | 2019-09-04 | Merck Patent GmbH | Method for detecting micro-colonies growing on a membrane or an agarose medium of a sample and a sterility testing apparatus |
FR3022916B1 (fr) * | 2014-06-30 | 2018-04-06 | Biomerieux | Procede de detection d'une presence ou d'une absence de particules biologiques. |
FR3028616A1 (fr) * | 2014-11-13 | 2016-05-20 | Commissariat Energie Atomique | Procede d'analyse comprenant la determination d'une position d'une particule biologique. |
CA2985854C (en) | 2015-04-23 | 2023-10-17 | Bd Kiestra B.V. | A method and system for automated microbial colony counting from streaked sample on plated media |
EP3286731B1 (en) | 2015-04-23 | 2020-09-16 | BD Kiestra B.V. | Colony contrast gathering |
FR3065556B1 (fr) | 2017-04-19 | 2020-11-06 | Tiempo | Circuit electronique securise par perturbation de son alimentation. |
CN107254505A (zh) * | 2017-07-17 | 2017-10-17 | 中国家用电器研究院 | 一种材料样块防霉性能的评价方法 |
EP3916379A1 (en) | 2020-05-29 | 2021-12-01 | Becton Dickinson France | Method for inspecting a medical container and system used therefor |
CN112525905B (zh) * | 2020-11-23 | 2022-09-30 | 西安建筑科技大学 | 基于背景纹影成像的污染气流强度识别、排放控制方法及系统 |
FR3144664B1 (fr) | 2022-12-28 | 2024-12-13 | Commissariat Energie Atomique | Dispositif d’observation d’un échantillon par imagerie |
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WO2008023024A1 (fr) * | 2006-08-23 | 2008-02-28 | Commissariat A L'energie Atomique | Procede de mesure sans contact d'objets tridimensionnels a deux couches par ombroscopie optique a une seule vue |
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US20060280352A1 (en) * | 2005-06-10 | 2006-12-14 | The Cleveland Clinic Foundation | Image analysis of biological objects |
WO2008023024A1 (fr) * | 2006-08-23 | 2008-02-28 | Commissariat A L'energie Atomique | Procede de mesure sans contact d'objets tridimensionnels a deux couches par ombroscopie optique a une seule vue |
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FR2958298A1 (fr) | 2011-10-07 |
EP2556164B1 (fr) | 2018-05-30 |
WO2011125033A1 (fr) | 2011-10-13 |
KR20130047691A (ko) | 2013-05-08 |
CN103025888A (zh) | 2013-04-03 |
BR112012025587B1 (pt) | 2019-05-28 |
JP5907947B2 (ja) | 2016-04-26 |
JP2013523147A (ja) | 2013-06-17 |
US20130084598A1 (en) | 2013-04-04 |
BR112012025587A2 (pt) | 2017-11-28 |
EP2556164A1 (fr) | 2013-02-13 |
RU2604794C2 (ru) | 2016-12-10 |
RU2012146989A (ru) | 2014-05-20 |
FR2958298B1 (fr) | 2014-10-17 |
CN103025888B (zh) | 2015-09-30 |
US8855397B2 (en) | 2014-10-07 |
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