KR101839826B1 - Corneocyte mimetics containing natural moisturizing factor, preparation method of the same, and cosmetic composition for moisturizing containing the same - Google Patents

Abstract

The present invention relates to a hydrogel particle on which a natural moisturizing factor is supported; And a lipid coating layer surrounding the hydrogel particles, and a process for producing the same. The keratinocyte cell line carrying the natural moisturizing factor of the present invention contains a large amount of binding water for a long time and exhibits excellent water retention ability and moisturizing effect and provides a moisturizing cosmetic composition containing it as a moisturizing ingredient.

Description

TECHNICAL FIELD The present invention relates to a keratinocyte having a natural moisturizing factor, a method for producing the keratinocyte, and a cosmetic composition containing the natural keratinocyte and a moisturizing cosmetic composition containing the natural moisturizing factor.

The present invention relates to a keratinocyte cell body carrying a natural moisturizing factor, a method for producing the same, and a cosmetic composition for moisturizing containing the same.

These days, as well as the changes in the environment and life patterns, such as anthropogenic temperature control of heating and cooling, various stresses caused by social life, skin stress caused by environmental pollution, frequent washing according to makeup habit, The moisture of the stratum corneum is decreased, and the skin becomes dry, the surface becomes rough, the skin becomes loose, the luster is lost and the skin looks dull, and the need for a skin moisturizer is increasing.

The skin is largely divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside. It has a function to protect the internal organs of the body from changes in temperature and humidity, ultraviolet rays, and other physical and chemical external stimuli. In particular, the epidermis plays an important role in preventing water evaporation inside the human body. The epidermis consists of stratum corneum, granular layer, superficial layer and basal layer in order from the outside. Cells of stratum corneum act like bricks, and intercellular lipids between keratinocytes act as mortar to constitute skin barrier. In addition, healthy keratinocytes contain a high concentration of natural moisturizing factor (NMF), which helps to retain moisture in the skin. For example, substances such as amino acids are water-soluble, Thereby suppressing drying.

Recently, it has been claimed that the differentiation of the stratum corneum is important for preventing the drying of the skin moisture. Therefore, the differentiation process developed from the keratinocytes of the basal layer to the keratinocytes of the outermost stratum corneum is normally performed, . In other words, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes rigid and supple, allowing it to function as a barrier. Physiological tendency of the skin to become dry as the age of the human is increased can be interpreted as a longer time for the removal of the stratum corneum, a lowering of the lipid synthesis ability of the epidermal cells, and a decrease in the amount of the moisturizing factor and lipid in the stratum corneum . Therefore, by promoting the differentiation of keratinocytes, a new approach to enhance skin barrier function and protection from external environment has been made possible by inducing reinforcement of skin barrier.

Until recently, most of the materials used as moisturizing agents were humectants which absorb moisture and occlusive moisturizers which prevent evaporation of water. Consequently, a method of increasing moisture retention in the stratum corneum has been performed. The humectant includes substances such as glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol and 2-pyrrolidone-5-carboxylate. However, when it is applied to the skin, There is a drawback to this. Lipid components such as ceramides, essential fatty acids and lipid complexes have been used as closed moisturizers, but they have difficulties in maintaining the stability of emulsified formulations and are not suitable for producing transparent gel-like products. In addition, studies on effective moisturizing agents that can simultaneously impart humectant and closed humectant functions are insignificant.

In order to solve the above-mentioned problems, the present invention relates to a method for improving moisture retention by supporting a high concentration of natural moisturizing factor similar to keratinocytes, and at the same time, it is surrounded by a lipid lamellar structure to prevent moisture evaporation, It is an object of the present invention to provide a keratinocyte carrying a visible natural moisturizing factor, a method for producing the same, and a cosmetic composition for moisturizing containing the same.

In order to achieve the above object, the present invention provides a hydrogel particle on which a natural moisturizing factor is supported; And a lipid coating layer surrounding the hydrogel particles.

(A) dissolving a lipid in an organic solvent;

(b) dispersing a natural moisturizing factor in an organic solvent in which the lipid is dissolved;

(c) dispersing the hydrogel particles prepared from the water-soluble polymer and the water-soluble crosslinking agent in the dispersion obtained in the step (b); And

(d) introducing water into the dispersion obtained in the step (c) and distillation under reduced pressure.

The present invention also provides a cosmetic composition comprising the keratinocyte as an active ingredient.

The keratinocyte cell line carrying the natural moisturizing factor of the present invention contains a large amount of binding water for a long time and exhibits excellent water retention ability and moisturizing effect and provides a moisturizing cosmetic composition containing it as a moisturizing ingredient.

1 is an optical microscope image (a) and a polarization microscope image (b) of a keratinocyte carrying a natural moisturizing factor according to an embodiment of the present invention.
FIG. 2 is a graph showing moisture content of a keratinocyte carrying a natural moisturizing factor according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail in order to facilitate the present invention by those skilled in the art.

The present invention relates to a hydrogel particle on which a natural moisturizing factor is supported; And a lipid coating layer surrounding the hydrogel particles.

A method for producing a keratinocyte of the present invention comprises: (a) dissolving a lipid in an organic solvent; (b) dispersing a natural moisturizing factor in an organic solvent in which the lipid is dissolved; (c) dispersing the hydrogel particles prepared from the water-soluble polymer and the water-soluble crosslinking agent in the dispersion obtained in the step (b); And (d) adding water to the dispersion obtained in the step (c) and distillation under reduced pressure.

Hereinafter, a method for producing a keratinocyte will be described in detail.

First, the step of (a) dissolving the lipid in the organic solvent is carried out.

In the step (a), the organic solvent may be an organic solvent; And C ₁ to C ₆ alcohols, said primary organic solvent being a non-polar solvent having a boiling point of from 40 to 110 ° C and being selected from the group consisting of hydrocarbons, halogen hydrocarbons and aromatic hydrocarbons, Methylene chloride, chloroform, hexane, heptane, isooctane, cyclohexane, benzene, toluene, xylene and dimethyl ether. The alcohol serves as an auxiliary surfactant which assists dissolution of the lipid and stably disperses the hydrogel hydrated in the main organic solvent. The alcohol may be a C ₁ to C ₆ alcohol, and, as a non-limiting example, methanol, ethanol, propanol may be used. The content of the alcohol is preferably 20 to 70% of the main organic solvent content. If the alcohol content is less than 20%, the lipid does not dissolve or phase separation occurs between the hydrated hydrogel and the organic solvent, and when the alcohol content exceeds 70%, particles are not formed and lipid agglomeration occurs.

In the step (a), the lipid may be at least one selected from the group consisting of soybean lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycol, and hydrogenated phosphatidylcholine. And preferably soybean lecithin, but is not limited thereto.

The content of the lipid is preferably 2 to 10 times the dry weight of the hydrogel. If the amount of lipid is less than 2 times, the amount of uncoated hydrogel is too much, and when the amount of lipid is 10 times or more, most of the liposome is formed.

Then, (b) the step of dispersing the natural moisturizing factor in the organic solvent in which the lipid is dissolved is proceeded.

The natural moisturizing factor (NMF) mixture is not limited to any mixture prepared in a composition ratio similar to that of a natural moisturizing factor present in the stratum corneum of human skin. And preferably at least one selected from the group consisting of amino acids, sodium pyrrolidone carboxylate, sodium lactate and urea, and more preferably amino acids. In this connection, examples of preferable composition ratios are shown in Table 1 below.

 Composition ratio of natural moisturizing factor Raw material name content(%) Sodium pyrrolidone carboxylate 12 Sodium lactate 5 Urea 7 Serine 18.2 Glycine 9.1 Arginine 3.2 Glutamic acid 2.3 Tyrosine 0.5 Alanine 6.6 Purified water To 100

The amino acid may be at least one selected from the group consisting of serine, glycine, arginine, glutamic acid, tyrosine and alanine, but is not limited thereto.

The content of the natural moisturizing factor is preferably 5 to 30% by weight based on the keratinocyte. When the natural moisturizing factor is less than 5% by weight, the moisturizing effect is insufficient. When the natural moisturizing factor is more than 30% by weight, unpleasant raw material odor peculiar to natural moisturizing factor is generated and hindering the formation of the structure, It goes crazy.

And (c) dispersing the hydrogel particles prepared from the water-soluble polymer and the water-soluble crosslinking agent in the dispersion obtained in the step (b).

In the step (c), the hydrogel particles are prepared by cross-linking the water-soluble polymer with a water-soluble crosslinking agent as microparticles or nanoparticles. The W / O emulsion is formed by mixing the oily phase in which the surfactant is dissolved and the water-soluble polymer and the water-soluble crosslinking agent-dissolved water phase to form a W / O emulsion and the final product is washed and washed several times to remove the surfactant.

The water-soluble polymer may be at least one selected from the group consisting of hyaluronic acid and salts thereof, polyvinyl alcohol, polyvinylpyrrolidone, maleic anhydride / vinyl ether copolymer, gelatin, alginate, hydroxyethyl methacrylate, cariguanan, hydroxyethylcellulose, Hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, carboxyvinyl copolymer, polyethylene oxide, polyethylene glycol, polyacrylamide, polyhydroxyethylmethacrylate, polydioxolane, polyacrylic acid, polyacrylic acid , Polyacrylamide, and polyvinyl chloride, but is not limited thereto.

The water-soluble crosslinking agent may be at least one selected from the group consisting of ethylene glycol, glycerin, polyoxyethylene glycol, calcium chloride, bisacrylamide, diarylphthalate, diaryl adipate, 1,4-butanediol diglycidyl ether, polyethylene glycol diglycidyl ether, Propylene glycol diglycidyl ether, butylene glycol diglycidyl ether, triglycerin diglycidyl ether, triaryl amine, and glyoxal, but not always limited thereto .

Finally, (d) water is added to the dispersion obtained in the step (c), and the step of performing vacuum distillation is performed. In the step (d), it is preferable to slowly introduce water, and the hydrogel swells due to the water to be added. When the organic solvent is removed by distillation under reduced pressure, lipids are spontaneously aligned on the surface of the hydrogel particles and coated thereon.

The keratinocyte of the present invention has a large amount of bound water carried by a natural moisturizing factor and simulates the structural characteristics of keratinocytes, which are surrounded by cornified envelope and intercellular lipids and reduce water loss will be. For this purpose, a lipid vesicle having a hydrogel as a core was prepared, and a natural moisturizing factor was carried therein. Hydrogels and natural moisturizing factors captured a large amount of binding water, and a lipid bilayer, which was arranged in a lamellar structure, To prevent the outflow of excellent moisturizing effect.

The present invention also provides a cosmetic composition comprising the keratinocyte as an active ingredient.

The content of the active ingredient is not particularly limited, but is preferably 0.1 to 20% by weight based on the total weight of the composition. When the active ingredient satisfies the above content, it can exhibit excellent efficacy without side effects.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing But the present invention is not limited thereto, and may be manufactured by any one or more formulations selected from the group consisting of a foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

In addition to the keratinocyte matrix prepared as described above, the cosmetic composition may further contain a component included in a functional additive and a general cosmetic composition. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The cosmetic composition of the present invention may further contain, in addition to the above-described functional additive, components contained in a general cosmetic composition as required. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

The present invention will be described in more detail through the following examples. However, the examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.

[Example 1] Production of keratinocyte having natural moisturizing factor

The keratinocyte having a natural moisturizing factor supported thereon was prepared by the manufacturing method according to the present invention. Example 1 was prepared with the composition shown in Table 2 below.

First, soybean lecithin was added to the organic solvent to dissolve it completely, and then the natural moisturizing factor was added to disperse it in the form of w / o emulsion. In order to make the weight of the natural moisturizing factor excluding purified water 63.9%, the natural moisturizing factor 25% was mixed with the purified water to adjust the solid content of 16%.

Thereafter, hyaluronic acid hydrogel particles were prepared. The hyaluronic acid hydrogel particles were prepared by dissolving 4.5 g of the surfactant A-P135 in 105 g of dodecane through a stirrer, adding 1.35 g of hyaluronic acid (number average molecular weight 1,500, 000) to 45 g of a 0.1 N aqueous sodium hydroxide solution, 1.3 g of butylene glycol diglycidyl ether was dissolved via a stirrer. The above-mentioned hyaluronic acid and a 0.1 N aqueous sodium hydroxide solution in which the water-soluble crosslinking agent was dissolved were slowly added to the dodecane in which the surfactant was dissolved, and the mixture was stirred and mixed at a speed of 7000 rpm for 10 minutes to prepare a w / o emulsion. And the mixture was heated to 60 ° C to conduct an initial crosslinking reaction while stirring to maintain the w / o emulsion. Acetic acid was added to neutralize the aqueous phase of the w / o emulsion while the temperature of the reactor was kept at room temperature while continuously stirring, and the crosslinking reaction was carried out while stirring the w / o emulsion at room temperature for 2 days. The crosslinked hyaluronic acid hydrogel nanoparticles were obtained in the aqueous solution of the w / o emulsion and washed and vacuum-dried at 90 DEG C for 24 hours.

The powdery hyaluronic acid hydrogel particles thus obtained were dispersed in a w / o emulsion in which the natural moisturizing factor was dispersed, and distilled water was added while stirring with a mixer. Subsequently, all of residual organic solvent was removed using a rotary evaporator to complete a keratinocyte.

[Comparative Example 1]

Comparative Example 1 was prepared in the same manner as in Example 1, except that a natural moisturizing factor was not included and a glycerin aqueous solution was included as in the composition ratio shown in Table 2 below.

[Comparative Example 2]

In Comparative Example 2, the hydrogel core vesicle was prepared by a known film hydration method. First, 5 g of soybean lecithin was placed in a round flask, and 15 g of chloroform was added to dissolve it. Then, the mixture was distilled under reduced pressure through a rotary evaporator to form a lipid bilayer on the bottom of the flask. Thereafter, hyaluronic acid hydrogel was dispersed in distilled water in which a natural moisturizing factor was dissolved and sufficiently swollen. Then, the resultant was put in a flask in which the lipid bilayer membrane was formed, and heated to a temperature of 50 ° C or higher.

[Comparative Example 3]

In Comparative Example 3, natural moisturizing factors were simply mixed in purified water to compare the evaporation behavior of natural moisturizing factors. The content of the natural moisturizing factor was 19%, so that the comparison of the water evaporation behavior according to the solid content (lipid, hydrogel, natural moisturizing factor) of Example 1 facilitates comparison of the final residual amount.

Component (% by weight) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Soybean lecithin 5 5 5 - Hyaluronic acid hydrogel particles One One One - Natural moisturizing factor (63.9%) 10 - 10 19 Glycerin aqueous solution (63.9%) - 10 - - ethanol 7 7 - - Methylene chloride 15 15 - - chloroform - - 15 - Purified water to 100 to 100 to 100 to 100

Preparation Example 1 Preparation of a cosmetic composition containing a keratinocyte

The following formulations are O / W formulations and gel formulations, in units of weight%.

division Component (content:% by weight) Example 2 Comparative Example 4 Oil component Stearic acid 2.5 2.5 Cetearyl alcohol 0.5 0.5 Glyceryl stearate / PEG-100 stearate 1.5 1.5 Cetyl octanoate 4.0 4.0 Isopropyl palmitate 4.0 4.0 2-octyldodecanol 4.0 4.0 Dimethicone 1.0 1.0 Cyclomethicone 3.0 3.0 Paraoxybenzoic acid ester Suitable amount Suitable amount Purified water To 100 To 100 Water component 1 Butylene glycol 5.0 5.0 Disodium EDTA Suitable amount Suitable amount TEA Suitable amount Suitable amount Imidazolidinyl urea Suitable amount Suitable amount Carbomer 1% aqueous solution 12 12 Water component 2 The keratinocyte model prepared in Example 1 5 -

division Component (content:% by weight) Example 3 Comparative Example 5 Water component 1 Purified water To 100 To 100 Butylene glycol 5.0 5.0 Disodium EDTA Suitable amount Suitable amount Trehalose One One PEG-75 One One Potassium hydroxide Suitable amount Suitable amount Water component 2 Carbomer 1% aqueous solution 20 20 Xanthan gum 0.1 0.1 Water component 3 The keratinocyte model prepared in Example 1 5.0 - Alcohol ingredient Ethanol 95% 3.0 3.0 Polyoxyethylene hydrogenated castor oil 0.3 0.3 Paraoxybenzoic acid ester Suitable amount Suitable amount

[Manufacturing method of Example 2 and Comparative example 4]

(1) The oil components were heated at 70 to 75 DEG C and mixed uniformly.

(2) Water component 1 was uniformly dissolved and mixed by heating at 70 to 75 ° C.

(3) The mixture of (1) above was added to the mixture of the above (2) under stirring at a temperature of 70 to 75 ° C to prepare an emulsion. After cooling to 50 ° C or lower, And dispersed uniformly.

[Manufacturing method of Example 3 and Comparative Example 5]

(1) Water component 1 was uniformly mixed.

(2) The alcohol components were uniformly mixed.

(3) Water component 2 was gradually added to the mixture of the above (1) with stirring, and after incrementing, the alcohol component was added continuously to prepare a gel formulation. Water component 3 was added and stirred to disperse uniformly.

[Test Example 1] Comparison of the carrying efficiency of natural moisturizing factors in a particle

50 ml of each of Example 1 and Comparative Example 2 was centrifuged at 5000 rpm for 10 minutes to analyze the amount of serine in the supernatant which was not supported on the particles by HPLC to predict the carrying efficiency of the natural moisturizing factor. As can be seen from Table 5, it was confirmed that the keratinocyte produced by the production method of the present invention had a 3 times higher loading efficiency of natural moisturizing factor than the conventional liposome adsorption method.

division Loading efficiency Example 1 12.6 ± 2.8% Comparative Example 2 2.8 ± 0.9%

[Test Example 2] Measurement of moisture evaporation behavior

The moisture evaporation behavior in the coating film formed by applying the example 1 and the comparative examples 1 to 3 was measured by measuring the weight change in real time immediately after application. Experiments were carried out under the condition of 25 ± 2 ℃ / 38 ± 1% by controlling the influence of temperature, humidity and wind by installing a desiccator (dehumidifier) inside the constant temperature and humidity chamber.

The experiment was carried out using artificial leather as a substitute for the skin, and 35 μl of the formulation on a 5 × ⁵ cm ² area was applied on a glass plate until the formulation was completely transparent under Shear Stress.

Immediately after application, we measured the weight change of the formulation film in 1 hour by LabX Light Balance 1.5 program connected with electronic scale (XS204V, Capacity: 220g, Readability: 0.1mg) Respectively. At least three replicate measurements were made on the same sample.

The moisture evaporation behavior of Example 1 and Comparative Examples 1 to 3 is shown in Fig. The analysis of the evaporation pattern was divided into an initial section in which the free water evaporated in the coated film and a linear change occurred, and a latter section showing an exponential change in evaporation of the bound water in the coated film. There are two indices that can be derived from the evaporation pattern: the residual amount after 1 hour, the final residual amount and the evaporation rate of the bound water, that is, the slope of the curve section.

These two indicators can be used to determine how much water each test group is constantly holding. Two indicators derived from Figure 2 are set forth in Table 6 below. As shown in Table 6, the final amount of residual was increased in the order of Comparative Example 3 <Comparative Example 1 <Comparative Example 2 <Example 1. This seems to be due to the fact that the final residual amount is directly related to the final water retention in the sample groups with the same solid content.

The slope of the curve section is represented by a value in the predicted equation (F = Y0 + a * exp ^{- bx} ) and is an index related to the evaporation rate of bound water. Example 1 <Comparative Example 2 <Comparative Example 1 < Respectively. That is, in Example 1, the evaporation rate of bound water is the slowest, and consequently, the final moisture content is large. In Comparative Example 1, which is a hydrogel core bezique carrying glycerin, which is a typical moisturizing ingredient in place of the natural moisturizing factor, glycerin shows dissolving power to lipid, so that the structure is hard to be maintained, and it is considered that it is difficult to effectively carry moisture into the structure. Comparative Example 2 is a hydrogel core vesicle prepared by a film hydration method. As shown in Test Example 1, it is considered that it is difficult to effectively support a natural moisturizing factor. Finally, Comparative Example 3, which is a natural moisturizing factor, is difficult to form a film that blocks evaporation of water, so that moisture evaporation is the fastest.

Index derived from moisture evaporation pattern division Final residual amount Curve slope Example 1 30.2 34.2 Comparative Example 1 15.4 36.1 Comparative Example 2 17 35.9 Comparative Example 3 14 60.9

[Test Example 3] Test for improving skin moisturizing power in human body

The effect of improving the skin moisturizing ability of the oil-in-water emulsion and the solubilized gel formulation prepared in Examples 2 and 3 and Comparative Examples 4 and 5 was measured.

Forty adult males and females, each representing a dry skin condition, were divided into four groups, and the cosmetic compositions of Examples 2 and 3 and Comparative Examples 4 and 5 were applied twice daily to each face for 4 weeks in each group. (24 ° C, relative humidity 40%) after 1 week, 2 weeks, and 4 weeks after application and before application and after 2 weeks (total 6 weeks) The skin moisture value was measured with a coneometer, a moisture meter for measuring the amount of water present in the skin by measuring the conductivity value. The results are shown in Table 7 below.

The results in Table 7 are the percentages (%) of the increase in measured values after treatment for a certain period of time based on the coneometer values measured immediately before the start of the test. As a result, it was confirmed that the water increase rates of Examples 2 and 3 were significantly higher than those of Comparative Examples 4 and 5.

Moisture growth rate according to application of cosmetic composition containing keratinocyte division Moisture growth rate (%) One week Two weeks 4th week 6th week Example 2 48 52 50 43 Example 3 27 30 32 32 Comparative Example 4 30 32 31 22 Comparative Example 5 19 24 20 15

Claims (15)

A cosmetic composition for use as a keratinocyte,
Wherein the composition comprises a keratinocyte as an active ingredient,
A hydrogel particle carrying a natural moisturizing factor; And
And a lipid coating layer surrounding the hydrogel particles,
The hydrogel particles are prepared by crosslinking a water-soluble polymer with a water-soluble crosslinking agent,
The water-soluble polymer may be at least one selected from the group consisting of hyaluronic acid and salts thereof, polyvinyl alcohol, polyvinylpyrrolidone, maleic anhydride / vinyl ether copolymer, gelatin, alginate, hydroxyethyl methacrylate, cariguanan, hydroxyethylcellulose, Hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, carboxyvinyl copolymer, polyethylene oxide, polyethylene glycol, polyacrylamide, polyhydroxyethylmethacrylate, polydioxolane, polyacrylic acid, polyacrylic acid , Polyacrylamide, and polyvinyl chloride, and at least one selected from the group consisting of
The water-soluble crosslinking agent may be at least one selected from the group consisting of ethylene glycol, glycerin, polyoxyethylene glycol, calcium chloride, bisacrylamide, diarylphthalate, diaryl adipate, 1,4-butanediol diglycidyl ether, polyethylene glycol diglycidyl ether, Wherein the cosmetic composition is at least one selected from the group consisting of propylene glycol diglycidyl ether, butylene glycol diglycidyl ether, triglycerine diglycidyl ether, triarylamine, and glyoxal. The method according to claim 1,
The lipid may be at least one selected from the group consisting of soybean lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycol, and hydrogenated phosphatidylcholine. Use cosmetic composition. The method according to claim 1,
Wherein the content of the lipid is 2 to 10 times the dry weight of the hydrogel. The method according to claim 1,
Wherein the natural moisturizing factor comprises at least one member selected from the group consisting of amino acid, sodium pyrrolidone carboxylate, sodium lactate and urea. 5. The method of claim 4,
Wherein the amino acid comprises at least one selected from the group consisting of serine, glycine, arginine, glutamic acid, tyrosine and alanine. The method according to claim 1,
Wherein the content of the natural moisturizing factor is 5 to 30% by weight based on the keratinocyte. (a) dissolving a lipid in an organic solvent;
(b) dispersing a natural moisturizing factor in an organic solvent in which the lipid is dissolved;
(c) dispersing the hydrogel particles prepared from the water-soluble polymer and the water-soluble crosslinking agent in the dispersion obtained in the step (b); And
(d) adding water to the dispersion obtained in the step (c) and distillation under reduced pressure. 8. The method of claim 7,
The lipid may be at least one selected from the group consisting of soybean lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycol, and hydrogenated phosphatidylcholine. A method of manufacturing a carcass. 8. The method of claim 7,
Wherein the content of the lipid is 2 to 10 times the dry weight of the hydrogel. 8. The method of claim 7,
Wherein the natural moisturizing factor comprises at least one member selected from the group consisting of amino acid, sodium pyrrolidone carboxylate, sodium lactate and urea. 11. The method of claim 10,
Wherein the amino acid comprises at least one selected from the group consisting of serine, glycine, arginine, glutamic acid, tyrosine and alanine. 8. The method of claim 7,
Wherein the content of the natural moisturizing factor is 5 to 30% by weight based on the keratinocyte. 8. The method of claim 7,
The water-soluble polymer may be at least one selected from the group consisting of hyaluronic acid and salts thereof, polyvinyl alcohol, polyvinylpyrrolidone, maleic anhydride / vinyl ether copolymer, gelatin, alginate, hydroxyethyl methacrylate, cariguanan, hydroxyethylcellulose, Hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, carboxyvinyl copolymer, polyethylene oxide, polyethylene glycol, polyacrylamide, polyhydroxyethylmethacrylate, polydioxolane, polyacrylic acid, polyacrylic acid , Polyacrylamide, and polyvinyl chloride. &Lt; RTI ID = 0.0 &gt; 11. &lt; / RTI &gt; 8. The method of claim 7,
The water-soluble crosslinking agent may be at least one selected from the group consisting of ethylene glycol, glycerin, polyoxyethylene glycol, calcium chloride, bisacrylamide, diarylphthalate, diaryl adipate, 1,4-butanediol diglycidyl ether, polyethylene glycol diglycidyl ether, Wherein the at least one compound is at least one selected from the group consisting of propylene glycol diglycidyl ether, butylene glycol diglycidyl ether, triglycerine diglycidyl ether, triarylamine and glyoxal. delete

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