KR101728125B1 - Method for manufacturing cosmetic composition containing porifera spicule - Google Patents

Abstract

The present invention relates to a production method of a cosmetic composition, containing Porifera spicule, wherein the production method comprises the steps of: a) preparing sponge Porifera powder by drying and crushing Porifera; b) mixing the Porifera powder with distilled water; c) reacting the mixture and enzyme in an enzyme reactor; d) centrifuging the reacted mixture and washing the same; e) heating the mixture of step d) in an electric furnace to obtain Porifera spicule ; and f) coating the Porifera spicule with a skin effective material.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing a cosmetic composition containing a sea surface skeleton,

The present invention relates to a method for producing a cosmetic composition containing a sea surface bone fragment, and more particularly, to a method for producing a cosmetic composition containing a sea surface skeleton purified using distilled water and an enzyme.

Porifera is a simple walkie-pharynx with about 1,000 species of lower animals without muscle, nervous system, digestive system and excretory system differentiation. The upstairs is made up of sympathetic cells, a unique organ of the sponges. It causes the water to flow, and when the seawater is introduced into the stomach through several small pores in the body wall (the cortex), it breathes and feeds the food. .

The fibrous skeleton of sponges is a form in which a small fragment (spicule) supports the body. The bone fragments are composed of calcareous or siliceous, and are divided into major and minor fragments.

The fragments of sponges have absorption ability by capillary phenomenon and are used for various purposes such as bathing, medical use, cosmetic use. Korean Patent Publication No. 2002-0041846 relates to a method for producing an antibiotic-containing sea surface bone fragments for treating osteomyelitis, Korean Patent Registration No. 10-0937389 discloses a method for producing a sponge bone comprising a step of extracting a micro needle from a spongy animal using a hydrogen peroxide solution, A method of manufacturing a skin care cosmetic composition using bone fragments is described.

Conventionally, a chemical purification process using an acid or a base has been used to utilize the sea surface fragment. However, when a chemical purification process is used, loss of trace metal elements contained in the bone fragments is significant, and irritation may be caused to the skin.

The present invention aims at purifying cancellous bone fragments by using enzymes without using a chemical purification process and utilizing them as a cosmetic composition.

The present invention relates to a method for producing a cosmetic composition containing a sea surface skeleton, comprising the steps of: a) preparing a sea surface powder by drying and crushing a sea surface animal; b) mixing the sea surface powder with distilled water; c) reacting the mixture and the enzyme with an enzyme reactor; d) centrifuging and washing the reacted mixture; e) heating the mixture of step d) in an electric furnace to obtain a sea level skeleton; And f) coating the sponge fragment with a skin-effective material.

Preferably, the enzyme is at least one selected from the group consisting of Protamex and Alcalase.

Preferably, step b) above is mixed with 3 to 5 ml of distilled water per g of sponge powder.

Preferably, the enzyme of step c) is reacted in an amount three to ten times that of the distilled water used.

Preferably, the sponge fragment has a size of 100 to 250 mu m.

The present invention also relates to a cosmetic comprising the cosmetic composition prepared by the process for producing a cosmetic composition containing the sea surface bone fragment.

Preferably, the cosmetic composition comprises 0.3 to 13% by weight of the total weight of the cosmetic.

The cosmetic composition containing the spongelike bone material produced according to the present invention is purified by using purified water and an enzyme without using chemical agents, so that there is no loss of trace metal elements and little skin irritation.

The cosmetic composition according to the present invention can directly, effectively and continuously deliver the active ingredient deep into the skin due to the spongy bone fragments. In other words, the continuous effect can be obtained by slowly penetrating the coated active substance into the skin due to the caudal bone fragments securing the capillary.

In addition, there are various effects such as promotion of skin cell regeneration, increase in moisturizing effect, antioxidative effect, and improvement of anti-inflammation by promoting metabolism by giving proper stimulation to skin with a sea level bone piece. Specifically, when the sea surface bone piece penetrates into the skin, a weak inflammatory reaction may occur, resulting in a physical effect of decolorization, and the absorbed micro-sized sea level bone piece absorbs the active ingredient into the skin to promote blood circulation, collagen formation, , Sedative and anti-inflammatory effects such as moisturizing, elasticity and various skin cell regeneration effects can be achieved.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a view showing that a central capillary tube of a sea surface bone piece according to the present invention is secured.
2 is a view showing a sea surface bone fragment purified according to the present invention.
Fig. 3 is a schematic view showing the action and effect when the cosmetic according to the present invention is applied to the skin.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The present invention relates to a method for producing a cosmetic composition containing a sea surface bone fragment. Specifically, the method comprises the steps of: a) preparing sponge powder by drying and crushing the sponge animal; b) mixing the sea surface powder with distilled water; c) reacting the mixture and the enzyme with an enzyme reactor; d) centrifuging and washing the reacted mixture; e) heating the mixture of step d) in an electric furnace to obtain a sea level skeleton; And f) coating the sponge fragment with a skin-effective material.

The first step is to prepare the sea surface powder by drying and crushing the sponge animal (step a). The sponge animal used in the present invention can be any spongy animal, but preferably a freshwater spongy animal can be used. The sponge animal is dried to have a water content of less than 10%, preferably less than 8%, and then ground with a homogenizer to prepare sponge powder. The sponge powder is preferably of a size such that it passes through a 50 to 60 mesh sieve net.

The second step is to mix the sea surface powder prepared in step a) with distilled water (step b). It is preferable to mix the sea surface powder and the distilled water with 3 to 5 ml of distilled water per 1 g of the sea surface powder. The stirring is preferably carried out to facilitate mixing, and the stirring temperature is preferably 0 ° C to 5 ° C and preferably 1 to 3 hours.

Next, the mixture of step b) and the enzyme are reacted with an enzyme reactor (step c). Specifically, the mixture is placed in a sealed pouch, and then enzyme is added to react with an enzyme reactor. The enzyme used in the present invention may be a protease, and preferably one or more selected from the group consisting of Protamex and Alcalase. ProTamex and Alkalase can be used alone, but they can be used in combination. In the case of mixing protamex and an alcalase, the mixture can be mixed at a weight ratio of 3: 1 to 1: 3, but it is preferable to mix the mixture at a weight ratio of 1: 1. The amount of enzyme used in the present invention may be 3 to 10 times, and more preferably 4 to 8 times, the distilled water used in step b). The reaction of the enzyme reactor can be carried out at a temperature of 40 to 50 DEG C for 20 to 30 hours under a pressure of 40 MPa to 80 MPa.

Next, the step (c) is a step (step d) of centrifuging and washing the enzyme-reacted mixture. After removing the enzyme-reacted mixture from the sealed pouch in step c), centrifuge the liquid mixture at about 15,000 rpm. After centrifugation, the supernatant is removed, washed with a washing solution and the solvent is removed. After washing with distilled water, the process of precipitation and removal of the supernatant may be carried out two to four times. The washing solution may be chloroform, methanol or a mixture thereof. The mixed solution may be mixed with chloroform and methanol at a weight ratio of 9: 1 to 1: 9, preferably 5: 5 to 7: 3.

Next, the step (d) is a step (step e) of heating the mixture in an electric furnace to obtain a sea level skeleton. The temperature of the electric furnace can be heated by gradually raising the temperature, thereby securing pores of the cancellous bone and removing residual organic matter. First, it is gradually heated at 100 ° C to 150 ° C for 20 minutes to 40 minutes, at 300 ° C to 400 ° C for 20 minutes to 40 minutes, and at 800 ° C to 900 ° C for 40 minutes to 1 hour. If heated sequentially, it is preferable to maintain the structural stability of the obtained fragments, and if rapidly heated, the moisture of the organic matter remaining in the porous body may expand, resulting in breakage or rupture of the fine needle structure.

Finally, it is a step (step f) of coating the sponge fragment obtained in step e) with a skin-effective substance. The coating step may be carried out by air-mixing while spraying the skin-active substance onto the sea surface bone fragments, but includes all the coating methods to achieve the object of the present invention. A skin-effective substance means any substance that can be contained in cosmetics, and means a substance that gives a useful effect to the skin. That is, the skin-active substance of the present invention is selected from the group consisting of skin conditioners, whitening agents, moisturizers, skin conditioners, acidity regulators, bactericides, antioxidants, stabilizers, solubilizers, chelating agents, vitamins, Or more species.

The size of the sea surface bone piece coated with the skin-effective substance may be 100 to 250 탆. When the size of the sea surface fragment is less than 100 탆, the capillary size is small and the efficiency of the coating material is low due to the small amount of the coating material. When the size exceeds 250 탆, the size of the sea surface fragment is large, have.

The present invention also relates to a cosmetic comprising the cosmetic composition comprising the sponge bone fragment. The cosmetic composition according to the present invention may be contained in an amount of 0.3 to 13% by weight based on the total weight of the cosmetic. When the cosmetic composition is contained in an amount of less than 0.3% by weight, the effect obtained by the sea surface bone fragment is insignificant. When the cosmetic composition is contained in an amount exceeding 13% by weight, skin irritation is serious and the economical efficiency is low.

The cosmetics according to the present invention can be prepared in any formulations conventionally produced in the art and can be used in various forms such as softening lotion, nutritional lotion, massage cream, nutritional cream, gel, pack, essence, lipstick, makeup base, foundation, Lotions, ointments, creams, patches, aerosols, sprays, powders, eye shadow and body cleansers.

Hereinafter, the present invention will be described in detail with reference to the drawings so that those skilled in the art can easily carry out the present invention.

1 (a) to 1 (c) are views showing that a central capillary tube of a sea surface bone piece according to the present invention is secured. The capillary of the sea surface skeleton contains the active ingredient and is slowly administered (released) after application to the skin, so that it can be seen that it is useful as a cosmetic product. As can be seen from FIG. 1, the sponge fragments according to the present invention show large and clear capillaries despite the absence of chemical purification.

Fig. 2 (a) is a view showing a tablet conveying skeleton piece, and Fig. 2 (b) is a view showing a sea surface skeleton purified according to the present invention. As can be clearly seen from FIG. 2, it can be seen that when purified according to the method of the present invention, highly purified sea surface bone fragment purified with few impurities can be obtained.

Fig. 3 is a schematic view showing the action and effect when the cosmetic according to the present invention is applied to the skin. When applied to the skin, the sea tangle fragments contained in the cosmetic penetrate into the skin, release useful substances slowly in the skin to activate the cells, promote collagen synthesis, and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1: Sponges The fragment  Containing Cosmetics  Preparation of composition

The sea surface collected from fresh water was dried and pulverized to a water content of 8%, and separated into 60 mesh sieve to prepare sponge powder. To 50 g of the sea surface powder prepared, 200 mL of distilled water was added, and the mixture was stirred at 4 캜 for about 2 hours. Next, the stirred mixture was placed in a sealed pouch, and 0.6 L of protease and 0.6 L of alkarazine were added. The conditions of the enzyme reactor were adjusted to 50 캜 and 60 MPa, and the enzyme reaction was carried out for about 24 hours. The mixture was centrifuged at 15,000 rpm after the enzymatic reaction of the sealed pouch. After centrifugation, the supernatant was removed, washed with 200 mL of a mixture of chloroform and methanol at a weight ratio of 7: 3, and then the solvent was removed. The process of washing with water and then precipitating was repeated 3 times. After that, the powder was placed in an electric furnace, and the temperature was successively elevated at 110 DEG C for 30 minutes, at 390 DEG C for 35 minutes, and at 800 DEG C for 55 minutes. Acetylhexapeptide-8 / palmitoylpentapeptide was spray-sprayed onto the sea tangle fragments obtained by the above process and air-mixed and coated.

Comparative Manufacturing Example  1: chemically purified sponges Skeletal  Produce

The sea surface collected from fresh water was dried to a moisture content of 8% and put into hydrogen peroxide. The solvent was then removed and dried to produce the powder form. Acetylhexapeptide-8 / palmitoylpentapeptide was spray-sprayed onto the sea tangle fragments obtained by the above process and air-mixed and coated.

Example  And Comparative Example  Produce

Example 1 including Preparation Example 1 containing sea surface fragments according to the present invention, Comparative Example 1 including the sea surface fragments chemically purified and Comparative Example 2 including untreated sea surface fragments are shown in the following Table 1 .

Example 1 Comparative Example 1 Comparative Example 2 Production Example 1 1.2 - - Comparative Preparation Example 1 - 1.2 - Undefined sea surface - - 1.2 glycerin 5 5 5 Cetyl hexanoate 3 3 3 Plant extract 2 2 2 Butylene glycol 2 2 2 Dipropylene glycol 4 4 4 Triethanolamine 0.05 0.05 0.05 Dimethicone One One One EDTA-2Na 0.02 0.02 0.02 Emulsifying stabilizer 2 2 2 Meadow Foam Seed Oil 2 2 2 Polysorbate 80 One One One Purified water to 100 to 100 to 100 Sum 100 100 100

Experimental Example  : Skin application and skin improvement sensory evaluation

Thirty women in their 30s to 50s were randomly divided into three groups, and sensory evaluations were carried out on skin applicability and skin improvement effect. The evaluation criteria are shown in Table 2, and the results are shown in Tables 3 and 4.

Sensory evaluation standard score Very good 7 good 6 Somewhat good 5 usually 4 Somewhat bad 3 Poor 2 Very bad One

Skin application sensory evaluation Example 1 Comparative Example 1 Comparative Example 2 Skin-washability 5.7 4.9 4.5 Skin absorbency 5.9 5.1 4.3 Skin irritation 5.1 4.5 3.6

Skin improvement effect Sensory evaluation Example 1 Comparative Example 1 Comparative Example 2 Exfoliation effect 6.0 5.1 4.7 Skin tone improvement effect 5.7 4.9 4.1

As can be seen from the results of Table 3 and Table 4, Example 1 according to the present invention showed excellent skin-spreading and absorbability and low skin irritation. In addition, the effect of eliminating the dead skin and improving the skin tone are excellent.

Claims (7)

A method for producing a cosmetic composition containing sea level skeleton,
a) preparing sponge powder by drying and crushing the sponge animal;
b) mixing the sea surface powder with distilled water;
c) reacting the mixture and the enzyme with an enzyme reactor;
d) centrifuging and washing the reacted mixture;
e) heating the mixture of step d) in an electric furnace to obtain a sea level skeleton; And
f) coating said sponge fragment with a skin effective material,
Wherein the enzyme is at least one selected from the group consisting of Protamex and Alcalase. The method according to claim 1,
Wherein said step (b) is a step of mixing with 3 to 5 ml of distilled water per g of sponge powder. The method according to claim 1,
Wherein the enzymes of step c) are reacted in an amount of 3 to 10 times the distilled water used. The method according to claim 1,
Wherein the sponge skeleton has a size of 100 to 250 占 퐉. A cosmetic comprising a cosmetic composition produced by the process according to any one of claims 1 to 4. 6. The method of claim 5,
Wherein the cosmetic composition comprises 0.3 to 13% by weight of the total weight of the cosmetic. delete

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