KR101716763B1 - Breeding Method for Crickets Comprising Ginsenoside Type Saponin and Polysaccharide from Panax ginseng and their Extracts and Food Composition - Google Patents

Breeding Method for Crickets Comprising Ginsenoside Type Saponin and Polysaccharide from Panax ginseng and their Extracts and Food Composition Download PDF

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KR101716763B1
KR101716763B1 KR1020150066834A KR20150066834A KR101716763B1 KR 101716763 B1 KR101716763 B1 KR 101716763B1 KR 1020150066834 A KR1020150066834 A KR 1020150066834A KR 20150066834 A KR20150066834 A KR 20150066834A KR 101716763 B1 KR101716763 B1 KR 101716763B1
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ginseng
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cricket
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feeding
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이삼구
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/168Steroids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/204Animal extracts

Abstract

The present invention relates to a method of raising a cricket containing ginsenoside-type saponin and a ginseng polysaccharide, and a food composition containing the cricket extract and the extract thereof raised by the method of raising the cricket. More particularly, And a cricket containing ginsenoside-type saponin and ginseng polysaccharide capable of producing crickets excellent in nutrition and function by containing ginsenoside-based saponin and ginseng polysaccharide in crickets by feeding ginseng in accordance with the step And a food composition containing the extract of the cricket raised by the feeding method.
The method for raising crickets containing ginsenoside-based saponin and ginseng polysaccharide according to the present invention comprises preparing a source of rice bran, ginseng and calcium, wherein the ginseng is prepared by pulverizing ginseng of 4-year-old root ginseng to 30 to 40 mesh, Wherein the anchovy powder is prepared by boiling broth and drying the remaining anchovy residue to 30 to 40 mesh, and the egg shell powder is pulverized into 20 to 30 mesh Preparing; Preparing a feed containing rice bran, ginseng and a calcium source prepared in the preparation step, feeding the feed to the cricket three times a day, and in the fifth instar period of the cricket, 3 to 5 parts by weight of the ginseng is added to 100 parts by weight of the rice bran , 5 to 7 parts by weight of a calcium source, and feeding the feed; A second feeding step of preparing and feeding a feed comprising 5 to 10 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran at the sixth instar of the cricket; A third feeding step of preparing and feeding a feed comprising 10 to 15 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran in the period 7 to 9 of the cricket; .

Description

TECHNICAL FIELD [0001] The present invention relates to a method for breeding crickets containing ginsenoside-type saponins and ginseng polysaccharides, and a cricket extract and a food composition containing the extracts of the crickets raised by the method for breeding crickets containing the ginsenoside-type saponin and the ginsenoside polysaccharide. and Food Composition}

The present invention relates to a method of raising a cricket containing ginsenoside-type saponin and a ginseng polysaccharide, and a food composition containing the cricket extract and the extract thereof raised by the method of raising the cricket. More particularly, And a cricket containing ginsenoside-type saponin and ginseng polysaccharide capable of producing crickets excellent in nutrition and function by containing ginsenoside-based saponin and ginseng polysaccharide in crickets by feeding ginseng in accordance with the step And a food composition containing the extract of the cricket raised by the feeding method.

Herbal medicine raw materials such as ginseng, Hwanggi, and myeonmundong have been widely used for the purpose of relieving diseases and healing in general, and ginseng has been used for many years as the most noble herbal medicine ingredient in the Far East. Ginseng is the broadest concept that includes both ginseng, white ginseng, red ginseng, wild ginseng, camphor, ginseng, raw ginseng, and taekguk ginseng. Through the history of long use, ginseng has been listed as one of the most important health functional food in Korea.

Ginseng contains 50-70% of carbohydrates, 10-15% of crude protein, 3-8% of crude saponin and 3-7% of ash, and the major physiologically active ingredients are saponin, phenolic component, polyacetylene component, Alkaloid components, polysaccharides and the like are known. Among them, ginseng saponin, which is called "ginsenoside", is named among the saponins of ginseng among the various saponins of plants. Ginseng saponin is a glycoside composed of glycone and aglycone. Up to now, about 30 species have been isolated and their structure is known.

The ginsenosides are classified into three classes of 37 kinds including diols, triols, and olefins according to their basic structures. Here, the diol system is sedated, and the triol system has an exciting function and an opposite function to each other, but plays a very important role in maintaining the homeostasis according to the state of the body. The diol system includes Rb1, Rb2, Rc, and Rd, and the triol system includes Re, Rf, Rg1, Rg2, and the like.

The R0 represents anti-inflammatory, detoxifying, antithrombin, platelet aggregation inhibiting, anti-hepatitis, and macrophage activation.

In addition, Rb1 may be used for the treatment of central nervous system depression, hypnotic action, analgesic action, mental stabilization action, antipyretic action, serum protein synthesis promoting action, neutral fat dissolving inhibiting action, synthesis promoting action (insulin similar action), cholesterol biosynthesis promoting action, RNA synthesis promoting action and hormone secretion promoting action, and Rb2 represents a central nerve inhibitory action, a DNA / RNA synthesis promoting action, a prasmin activating action, a corticosteroid stimulating hormone secretion promoting action, an anti-diabetic action, , RNA synthesis inhibitory action, serum protein synthesis promoting action, prasmin activating action, and adrenocorticotropic hormone secretion promoting action, and Rd exhibits action promoting adrenocortical hormone secretion.

In addition, Re shows central nerve inhibitory action, DNA / RNA promoting action, plasmin activating action, promoting adrenocorticotropic hormone secretion promoting action, Rf shows analgesic action related to neuronal cells, Rg1 is central nerve excitatory action, , Fatigue recovery, memory, learning function improving action, DNA · RNA synthesis promoting action, and prasmin activating action. Rg2 shows platelet aggregation inhibiting action and plasmin activating action.

Rh1 also exhibits anticancer activity, platelet aggregation inhibition and hepatoprotective actions, and Rh3 exhibits liver-detoxifying activity.

Recently, it has been found that various non-saponin fractions other than the saponin component have various pharmacological activities. Therefore, researches on pharmacological activity focusing on these active fractions have been variously developed.

Non-saponins include essential oils, phytosterols, polyacetylenes, polyphenols, flavonoids, polysaccharides, alkaloids, vitamins, minerals and enzymes. .

The polysaccharide is a pure polysaccharide composed of glucan (polysaccharide composed of glucose) and fructan (polysaccharide composed of fructan) and is a nano-polymer substance having a molecular weight of about 5,000 to 15,000. Promotes the regeneration and differentiation of peripheral blood cells as well as bone marrow cells, thereby promoting hematopoiesis and antimicrobial action and reducing side effects caused by radiation therapy. In addition, activation of macrophages promotes the production of interleukin-12, thereby activating the production of interferon by Th1-lymphocytes, thereby increasing the ability of natural killer cells (NK-Cell) and enhancing the cellular immune response (CTL) . In addition, it has been reported that it is effective for diseases such as diabetes by lowering blood sugar.

On the other hand, insects are mainly regarded as insect pests and have been focused on controlling them. However, studies on insect insects have not been studied yet. In recent years, some insects have been used to industrially use insect bioactive materials or to develop pharmaceuticals while newly recognizing the diversity and characteristics of insects.

Among them, crickets live around the islands and have habit of hiding in grassland or under the gardens of the garden, and the area of males is set. Most of them live on the ground, but they live on water, in the house, and in the house of ants. I put the ovipositor in the ground and spawn, and it dries in the ground in an egg state. It appears once a year from the middle of August to the end of October. It is generally omnivorous and rotten grass as a stock, but it also carnivorous, such as eating a small insect or the like, as well as a fountain. It also eats corpses such as insects and earthworms. In addition, the generation is short and it is evaluated as a useful biomass which is more industrialized in terms of time and economy than other biological resources.

Specifically, crickets are rich in unsaturated fatty acids including omega-3 fatty acids, and contain high levels of protein and chitin / chitosan. They are added to some animal feeds or gradually used as human high-protein foods. Development is necessary.

Korean Patent Publication No. 10-2013-0017746 (2013.02.20.)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a ginseng saponin and a ginseng polysaccharide in crickets by feeding ginseng in accordance with the growth stage of crickets, The present invention relates to a method for raising crickets containing ginsenoside-type saponin and ginseng polysaccharide, which can produce crickets excellent in nutrition and function, and to provide a food composition containing the cricket extract and its extracts raised by the raising method .

In order to achieve the above object, the present invention provides a source of rice bran, ginseng and calcium, wherein the ginseng is prepared by pulverizing ginseng of 4-year-old root ginseng to 30 to 40 mesh, wherein the calcium source is anchovy powder or egg shell powder The anchovy powder is prepared by boiling the broth and drying the remaining anchovy residue to 30 to 40 mesh, and the egg shell powder is prepared by pulverizing the egg shell into 20 to 30 mesh; Preparing a feed containing rice bran, ginseng and a calcium source prepared in the preparation step, feeding the feed to the cricket three times a day, and in the fifth instar period of the cricket, 3 to 5 parts by weight of the ginseng is added to 100 parts by weight of the rice bran , 5 to 7 parts by weight of a calcium source, and feeding the feed; A second feeding step of preparing and feeding a feed comprising 5 to 10 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran at the sixth instar of the cricket; A third feeding step of preparing and feeding a feed comprising 10 to 15 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran in the period 7 to 9 of the cricket; .

The method of breeding crickets containing the ginsenoside-based saponin and the ginseng polysaccharide according to the present invention and the food composition containing the cricket extract and the extract thereof raised by the method of breeding according to the present invention, The effect of adding ginseng and the content of saponin in crickets can be maximized.

In addition, the crickets raised by the breeding method described above contain ginsenoside-based saponin and ginsenoside polysaccharide in the cricket as the ginseng is ingested. As a result, the ginsenoside-containing saponins are more effective than the crickets in enhancing immunity, It has the effect of enhancing the anti-stress, anti-fatigue, memory improvement, skin beauty and cancer cell kill ability by ginseng polysaccharide, influenza virus and bacterial infection inhibiting action, liver function improving action and the like.

In addition, the cricket containing the ginsenoside-based saponin and the ginseng polysaccharide of the present invention promotes growth of the cricket by feeding the nutrient-rich ginseng at the time when the ginseng is important, and particularly, The hatching rate is increased by 50 ~ 70%.

In addition, since crickets with excellent nutrition and function can be mass-produced, it can contribute to increase in income of the production farmers.

Hereinafter, preferred embodiments of the present invention will be described in detail. These examples are for further illustrating the present invention, and the scope of rights of the present invention is not limited to these examples.

The method for raising crickets containing ginsenoside-based saponin and ginseng polysaccharide according to the present invention comprises the steps of preparing a source of rice bran, ginseng, and calcium; Preparing a feed containing the rice bran, ginseng, and calcium source prepared in the preparation step, and feeding the feed to the cricket three times a day, wherein the amount of the ginseng is varied according to the growth stage of the cricket; A second feeding step; A third pay step; .

More specifically, the ginseng is prepared by pulverizing ginseng of 4-year-old root ginseng to 30 to 40 mesh. The reason why the ginseng is used as a 4-year-old root is that the 4-year-old ginseng has a higher saponin content than the 5-year-old or 6-year-old root, as shown in Table 1 below. As shown in Table 2, the saponin content of the bottom portion of the ginseng is higher than that of the other portions of the ginseng, so that the present invention can increase the saponin content of crickets by using 4 years old ginseng.

(Table 1) Saponin content by lotus root (%)

Figure 112015046016311-pat00001

(Table 2) Saponin content per part (mg / g)

Figure 112015046016311-pat00002

The calcium source is composed of at least one of anchovy powder and egg shell powder. The anchovy powder is prepared by boiling the broth and drying the remaining anchovy residue to 30 to 40 mesh. The egg shell powder comprises 20% To 30 mesh.

Next, the feed containing the rice bran, ginseng, and calcium source is prepared and fed to the cricket three times a day. In the fifth period of the cricket, 3 to 5 parts by weight of the ginseng, 5 to 7 parts by weight of a feed is prepared and fed.

The reason why the first salivation step is started at the fifth instar period is that since the effect of the ginseng is slightly expressed before the fifth instar, it is preferable that the insemination is performed from the fifth instar period when the significant effect is exhibited.

In addition, if the amount of the ginseng contained in the feed of the fifth age group is more than 5 parts by weight, the effect of increasing the weight of ginseng is not significantly increased, so that the raw material can be wasted. When the amount is less than 3 parts by weight, the content of saponin in the cricket decreases .

If the amount of the calcium source is more than 7 parts by weight, adverse effects due to excessive calcium intake may occur. If the amount is less than 5 parts by weight, the effect of calcium may be deteriorated.

Next, during the sixth instar of the cricket, a second feeding step of preparing and feeding a feed comprising 5 to 10 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran is performed.

Next, in the 7th to 9th orders of the cricket, a third feeding step in which a feed comprising 10 to 15 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source is prepared and fed to 100 parts by weight of the rice bran. At this time, the 7th to 9th orders are characterized by having the highest content of ginseng in the adult stage.

If the ginseng content exceeds the maximum value, the effect of weight gain may not be increased and the saponin content in the cricket may decrease when the ginseng content is below the minimum value, as in the first feeding step. There is an effect that the effect and the saponin content can be maximized by feeding the ginseng content suitable for each period.

As a result, the crickets raised by the above breeding method have an effect of containing ginsenoside-based saponin and ginseng polysaccharide in the cricket as the ginseng is ingested. Therefore, the cricket of the present invention is more effective than the cricket in general, because it has the ability to enhance immunity by ginsenoside saponins, anticancer, anti-diabetic, blood flow improvement, antistress, anti-fatigue, memory, skin cancer and cancer cell killings by ginseng polysaccharide, And bacterial infection-suppressing action, and liver function-improving action.

In addition, the cricket containing the ginsenoside-based saponin and the ginseng polysaccharide of the present invention promotes growth of the cricket by feeding the nutrient-rich ginseng at the time when the ginseng is important, and particularly, And the hatching rate is improved by 50 to 70%.

As a result, the breeding method can produce crickets with excellent nutrition and function, thereby contributing to an increase in income of the production farmers.

On the other hand, crickets raised by the breeding method were subjected to hot water extraction by adding 10 times as much weight of distilled water as the cricket weight, and the obtained hot water extract solution was concentrated at a ratio of 1/50 to 1/60, followed by lyophilization. And a concentration of 250 to 350 mg / kg to prepare cricket extract containing ginsenoside-based saponin and ginseng polysaccharide.

In addition, the food composition containing the extract may be prepared in various forms including powders, pills, and capsules, and may be easily ingested.

< Example  1> Ginsenoside system  Saponin and Ginseng polysaccharide  Breeding crickets containing

First, rice ginseng, ginseng, and calcium sources were prepared. The ginseng was prepared by pulverizing 4-year-old ginseng root with 30 mesh. The calcium source was prepared by boiling broth, drying the remaining anchovy residue, Prepare an egg shell powder that has been pulverized into 30 mesh shells.

5 parts by weight of the ginseng, 5 parts by weight of the anchovy powder 3, and 3 parts by weight of the ginseng powder were added to 100 parts by weight of the rice bran at the fifth instar period of the cricket, And 3 parts by weight of the egg shell powder are prepared and fed to the first feeding step.

In the sixth period of the cricket, a second feeding step of preparing and feeding a feed comprising 100 parts by weight of the rice husk, 10 parts by weight of the ginseng, 3 parts by weight of the anchovy powder and 3 parts by weight of the egg shell powder is performed .

A third feeding step of preparing and feeding a feed comprising 15 parts by weight of the ginseng, 3 parts by weight of the anchovy powder and 3 parts by weight of the egg shell powder to 100 parts by weight of the rice bran is carried out at the 7th to 9th instar periods of the cricket do.

< Test Example  1> Comparison of spawning and hatching rate

In order to compare the scattering and hatching rates of the crickets raised in Example 1, the crickets of Example 1 and Comparative Example 1 were placed at a temperature of 30 ° C, a humidity of 60% 10 hours of illumination, 14 hours of darkness.

As a result, in Example 1, male mating was very active after adultization, and it was confirmed that active mating was in progress through the number of male cries for mating, and many spawning occurred when a medium for mass scattering after mating was introduced, The number of hatching in the hatching medium having the width x height x 20 cm x 10 cm x 3 cm when the medium was transferred to the incubation chamber after the spawning was counted as shown in Table 3 below.

(Table 3) Number of hatching (horses)

Figure 112015046016311-pat00003

As shown in Table 3, in the hatching medium of width x height x height 20 cm x 10 cm x 3 cm, the hatching number of each hatch was 32,472 in Example 1 and 20,648 in Comparative Example 1, Which was about 57% higher than that of the control group.

Thus, the method of raising crickets containing the ginsenoside-type saponin and the ginseng polysaccharide of the present invention has an effect of contributing to an increase in income of a production farm by enhancing the scattering and hatching rate by 50 to 70%.

< Test Example  2> Ginsenoside system  Saponin and Ginseng polysaccharide  analysis

The crickets raised in Example 1 and the ginsenoside-containing saponins and ginseng polysaccharides of Comparative Example 1, which is a general cricket, were analyzed.

The analytical method of the ginsenoside-based saponin was performed by freezing the cricket in an HPLC method, lyophilizing it in a freeze dryer at a temperature of -30 to -38 ° C, pulverizing it to 30 to 40 mesh to prepare a cricket powder, After adding 10 times 70% methanol based on the weight of the powder, it was extracted three times by reflux in a constant temperature water bath at 80 캜 and dried under reduced pressure. Water was added thereto, diethyl ether was added to remove fat-soluble components, extracted three times with water-saturated n-butanol, and concentrated under reduced pressure. Methanol was used as the final injection solvent. Then, the presence or absence of ginsenoside-based saponin was analyzed using a reversed phase column (C18) and a UV detector (PerkinElmer Series 200 HPLC, 203 nm).

The analytical method of the ginseng polysaccharide was performed by extracting water-soluble extracts three times for 8 hours with 8 times the amount of the cricket powder, lyophilizing the extract, and dissolving the extract in methanol to separate the methanol solubles and the non-solubles. Methanol solubles were excluded because they showed cytotoxicity against mouse normal lymphocytes. The methanol non-soluble matter (yield: 10.1%) was dissolved again in distilled water, and three times the amount of ethanol was added thereto to separate the ethanol-soluble matter (yield: 4.1%) and the ethanol-non-soluble matter (yield: 6.4% Was dialyzed with distilled water using a cellulose dialysis membrane. The dialysis was performed for 1 week at 4 ° C by changing the distilled water every 12 hours. The inside of the dialysis membrane was collected by lyophilization (yield: 3.5%) and the lyophilized product was subjected to Sephacryl S200 column chromatography. The column (2.5x90 cm) was filled with the filler, and the flow rate was adjusted so that the eluate was discharged 1 ml per minute, and 100 eluates were collected in 10 ml increments. Glucose and galactose, which constitute the ginseng polysaccharide, were detected by an anthrone-sulfuric acid reaction, and galacturonic acid, which is an acidic sugar, was measured for uronic acid content Lt; / RTI &gt;

(Table 4) Analysis results of ginsenoside-type saponin and ginseng polysaccharide

Figure 112015046016311-pat00004

(o: detected, -: not detected)

As shown in Table 4, the crickets raised by the method of breeding crickets containing the ginsenoside-based saponin and the ginseng polysaccharide of the present invention contain many ginsenoside-based saponins and ginseng polysaccharides as compared with general crickets appear.

The ginsenoside-based saponin R0 contained in the cricket of the present invention exhibits anti-inflammatory action, detoxifying action, antithrombin action, platelet aggregation inhibitory action, anti-hepatitis action and macrophage activation action.

In addition, Rb1 may be used for the treatment of central nervous system depression, hypnotic action, analgesic action, mental stabilization action, antipyretic action, serum protein synthesis promoting action, neutral fat dissolving inhibiting action, synthesis promoting action (insulin similar action), cholesterol biosynthesis promoting action, RNA synthesis promoting action and hormone secretion promoting action, and Rb2 represents a central nerve inhibitory action, a DNA / RNA synthesis promoting action, a prasmin activating action, a corticosteroid stimulating hormone secretion promoting action, an anti-diabetic action, , RNA synthesis inhibitory action, serum protein synthesis promoting action, prasmin activating action, and adrenocorticotropic hormone secretion promoting action, and Rd exhibits action promoting adrenocortical hormone secretion.

In addition, Re shows central nerve inhibitory action, DNA / RNA promoting action, plasmin activating action, promoting adrenocorticotropic hormone secretion promoting action, Rf shows analgesic action related to neuronal cells, Rg1 is central nerve excitatory action, , Fatigue recovery, memory, learning function improving action, DNA · RNA synthesis promoting action, and prasmin activating action. Rg2 shows platelet aggregation inhibiting action and plasmin activating action.

Rh1 also exhibits anticancer activity, platelet aggregation inhibition and hepatoprotective actions, and Rh3 exhibits liver-detoxifying activity.

In addition, the ginseng polysaccharide contained in the cricket of the present invention is useful as an anti-inflammatory agent for preventing and treating diseases such as cancer cell killing ability, influenza virus and bacterial infection inhibiting action, liver function improving action, triglyceride inhibition, antihyperlipidemic effect, immune enhancement, .

Thus, the cricket of the present invention containing the ginsenoside-based saponin and the ginseng polysaccharide can exhibit various healthful effects as described above.

Claims (4)

Wherein the calcium source is at least one of an anchovy powder and an egg shell powder, wherein the anchovy powder is prepared by mixing at least one of anchovy powder, Boiling the broth and drying the remaining anchovy residue to 30 to 40 mesh, and preparing the egg shell powder by pulverizing the egg shell into 20 to 30 mesh;

Preparing a feed containing rice bran, ginseng and a calcium source prepared in the preparation step, feeding the feed to the cricket three times a day, and in the fifth instar period of the cricket, 3 to 5 parts by weight of the ginseng is added to 100 parts by weight of the rice bran , 5 to 7 parts by weight of a calcium source, and feeding the feed;

A second feeding step of preparing and feeding a feed comprising 5 to 10 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran at the sixth instar of the cricket;

A third feeding step of preparing and feeding a feed comprising 10 to 15 parts by weight of the ginseng and 5 to 7 parts by weight of a calcium source to 100 parts by weight of the rice bran in the period 7 to 9 of the cricket; A method for breeding crickets containing ginsenoside-based saponins and ginseng polysaccharides.
delete A method for producing a plant, which is raised by the method of claim 1,
A cricket containing the ginsenoside-based saponin and ginseng polysaccharide and having a scattering and hatching rate of 50 to 70%
The extract obtained by hot-water extraction with 10 times the weight of distilled water and concentrating the obtained hot-water extract solution at a ratio of 1/50 to 1/60, followed by lyophilization, has an effective concentration of 250 to 350 mg / kg Extract of cricket containing ginsenoside-based saponin and ginseng polysaccharide.
A food composition comprising a cricket extract containing ginsenoside-type saponin and ginseng polysaccharide including the form of a powder, a ring and a capsule containing the extract of claim 3.
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