KR100599460B1 - Food composition comprising of extracts of cricket having protective effects against hepatotoxicity - Google Patents

Abstract

The present invention relates to a food comprising a cricket extract having a preventive effect on hepatotoxicity caused by a toxic compound such as dioxin, the extract is dried adult 500g of subtropical crickets of the genus Gryllus bimaculatus The hot water extract in 4,000 to 6,000 mL of distilled water, and the obtained hot water extract is concentrated at a ratio of 1/50 to 1/55, and then the extract obtained by lyophilization comprises at least 200 mg / kg to an effective concentration It provides a food comprising a cricket extract having a preventive effect on liver toxicity.

Cricket, extract, hepatotoxicity, dioxin, TCDD

Description

Food composition comprising of extracts of cricket having protective effects against hepatotoxicity

1 is a graph showing the results of experiments in the administration of the cricket extract in weight loss due to hepatotoxicity induced by dioxin according to the present invention.

Figure 2 is a graph showing the results of experiments in the administration of the cricket extract in blood biochemical changes due to hepatotoxicity induced by dioxin according to the present invention.

Figure 3 is a graph showing the results of experiments in the administration of the cricket extract in the change in relative organ weight due to hepatotoxicity induced by dioxin according to the present invention.

Figure 4 in the embodiments of the present invention stained liver tissue of rats of the G1 group (group orally administered to rats only saline containing no cricket extract without TCDD treatment) with hematoxylin / eosin staining reagent and 100 times 4 (A) shows the liver defibrillation and FIG. 4 (B) shows the central artery part.

Figure 5 in the embodiments of the present invention the liver tissue of the G2 group (oral administration of physiological saline not containing the cricket extract, and then injected with TCDD) stained with hematoxylin / eosin staining reagent 5 (A) is an enlarged photograph at 100 times magnification, and FIG. 5 (B) is an enlarged photograph at 200 magnifications.

Figure 6 in the embodiments of the present invention the liver tissue of the G4 group (Cricket extract 100mg / kg orally administered to the rat, then injected with TCDD) mice stained with hematoxylin / eosin staining reagent and 100 6 (A) shows the liver defibrillation and FIG. 6 (B) shows the central artery part.

Figure 7 in the embodiments of the present invention the liver tissue of rats of the G5 group (Cricket extract 200mg / kg orally administered to the rat, then injected with TCDD) mice stained with hematoxylin / eosin staining reagent 100 6 (A) shows the liver defibrillation and FIG. 6 (B) shows the central artery part.

The present invention relates to a food comprising a cricket extract having a preventive effect on hepatotoxicity. More specifically, the present invention relates to a food comprising a cricket extract having a protective effect against hepatotoxicity caused by toxic compounds such as dioxins.

Dioxins began to be a problem in Korea in the early 1990s when dioxin was contained as an impurity in the herbicide known as the defoliant in the Vietnam War, and various health disorders appeared to veterans and their descendants. In recent years, the waste incinerator has attracted the attention of citizens due to the excessive leakage of dioxins, and in April 1997, fish and shellfish in Masan Bay were detected with dioxin, 310 times higher than the specified value.

Dioxin (dioxin) refers to a compound having several chlorine attached to an aromatic compound having three rings, a polychlorinated dibenzo-p-dioxin (PCDD) having two oxygen atoms in the middle ring and a furan having one oxygen atom. All compounds are called polychlorinated dibenzo furan (PCDF). Depending on the number and position of the substituted chlorine, there are 75 isoforms in PCDDs and 135 isoforms in PCDFs, so there are 210 isomers in total dioxins. PCDDs are almost semi-permanent in our environment and contain various chemicals such as herbicides, pesticides, toxic fumes from waste incinerators, and polluted water from paper mills. By threatening the future of humanity.

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is one of the homologs of PCDDs and is known to cause the strongest toxicity in the body among the dioxins known to date (Pohjanvirta R & Tuomisto J (1994); Short-term toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in laboratory animals; effects, mechanism, and animal models.Pharmacol. Rev. 46; 483-549). TCDD is partly excreted in urine and bile, but it is fat-soluble and accumulates mainly in adipose tissue in the body, and has a chemically stable property, so it is hardly decomposed in nature (about 11 years of half-life in the body). It is even more dangerous because humans ingest things. Toxicity by TCDD is known to cause cancer in various organs, including skin, lungs, etc. in rodents, and accumulates more in the liver than fatty tissue at high concentrations (30 μg / kg or more). In particular, it has been reported to cause problems as a potent carcinogen in the rodent liver (Pitot HC, Goldsworthy T, Campbell HA & Poland A (1980); Quantitative evaluation of the promotion by 2,3,7,8-tetrachlorodibenzo-p-). dioxin of hepatocarcinogenesis from diethylnitrosamine.Cancer Res. 40; 3616-3620).

This suggests that TCDD induces hepatotoxicity by affecting cytochrome P-450 enzyme system, which is important for liver metabolism, and proliferation of liver cells and damage of oxidative DNA.

On the other hand, insects were mainly recognized as pests and focused on controlling them, but studies on useful insects are still insufficient. Recently, some insects have been used to industrially use the bioactive substances of insects or to develop medicines while newly recognizing the diversity and characteristics of insects.

Cricket extract is rich in unsaturated fatty acids including omega-3 fatty acids and contains high concentrations of protein and chitin / chitosan. Therefore, when the cricket extract is added to animal feed, the polyunsaturated fatty acid is increased in poultry, a small increase in EPA and Ahn MY, Ryu KS, Park BY, Kim DW, Kim I & Kim SH (2000); Effect of cricket on the chicken and its egg.Korean J. Poult.Sci. 27; 197-202 In addition, we reported the effects of cricket extract on the liver protection and swimming ability in acute liver injury induced by carbon tetrachloride (Ahn MY, Lee YW, Ryu KS, Lee HS, Kim I, Kim JW, Lee YK). , Kim ES & Kim YS (2002); Protective effects of water / methanol extracts of cricket on the acute hepatic damages in the ICR-mice induced by administration of CCl 4.Korean J. Food Sci.Technol. 34 (4); 684-; 687).

An object of the present invention is to provide a food comprising a cricket extract having a preventive effect on hepatotoxicity caused by toxic compounds such as dioxins.

Food containing a cricket extract having a preventive effect on hepatotoxicity according to the present invention, the dried adult 500g of subtropical crickets of the genus Gryllus bimaculatus hot water extracted in distilled water 4,000 to 6,000ml, The hot water extract is concentrated at a ratio of 1/50 to 1/55, and the extract obtained by lyophilization comprises at least 200 mg / kg as an effective concentration.

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Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Food comprising a cricket extract having a preventive effect on hepatotoxicity according to the present invention, the dried adult 500g of subtropical crickets of the genus Gryllus bimaculatus hot water extracted in distilled water 4,000 to 6,000ml, The hot water extract is concentrated at a ratio of 1/50 to 1/55, and the extract obtained by lyophilization is characterized in that it comprises at least 200 mg / kg to an effective concentration. Crickets are insects belonging to Gryllidae, which are commonly found around our living environment, and in the present invention, the subtropical crickets, especially the scientific name Gryllus bimaculatus, are used.

Although it is known as an emotional insect because of the sound of rubbing wings at summer night, the king cricket is a pest that eats fruit trees and various seedlings, but it is effective when baked and used in children's games. In Ahimagen and Oita, Japan, adults were used for diarrhea and dysentery. In typhoids, the adult juices were pressed by drinking them, and it was known that there was a custom to use extract as an antipyretic. However, in general, it is not commercially available, and crickets are currently sold in the United States at Zoomed as feed for reptiles and fish. In the present invention, it was found that the hot water extract of cricket can prevent hepatotoxicity caused by toxic substances, and it is characterized as a complete use invention by determining the effective concentration effective for hepatotoxicity through biochemical experiments. In the production of the cricket extract, if the distilled water for hot water extraction for less than 500g of the dried adult crickets, there may be a problem that the extraction of the active substance does not occur sufficiently, in the case of exceeding 6,000ml, heating, etc. It takes a lot of time, effort and energy to extract the hot water for economical, there may be a problem that productivity is lowered. Further, in order to simplify storage, handling, etc., the obtained hot water extract is concentrated at a ratio of 1/50 to 1/55, and then lyophilized.

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The effective concentration of cricket extract in the food is experimentally confirmed in the following Examples and Experimental Examples, and is a concentration suitable and effective to prevent hepatotoxicity caused by toxic compounds per body weight of the subject. If less, there may be a problem that the desired effect of preventing hepatotoxicity is not sufficient.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Example

500 g of dried adult crickets were extracted with hot water in 5,000 ml of distilled water, and the obtained hot water extract was concentrated at a ratio of 1/50, and then lyophilized to obtain a cricket extract.

Experimental Example

Storage and Handling of Test Substances

Test substance (cricket extract of the above example) was stored in an airtight container.

Hepatotoxic substances

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was used as a hepatotoxic agent. The hepatotoxic agents were stored in brown reagent bottles and refrigerated.

The test system purchased 40 Sprague-Dawley (SD) rats (Sampacobio Korea, South Korea) 6 weeks of age (190 to 200g) and raised for 7 days, and then raised them. 35 rats of age (209-223 g) were selected and used. SD rats are widely used in general toxicity and pharmacological tests, and a lot of basic data are available for reference.

Test group composition

The test group was configured as shown in Table 1 below.

division group TCDD Dose (µg / kg) Extract dose (mg / kg) Number of individuals G1 Negative Control 0 0 7 G2 TCDD-only group 60 0 7 G3 TCDD + extract low dose group 60 50 7 G4 TCDD + extract medium dose group 60 100 7 G5 TCDD + extract high dose group 60 200 7

Route of administration

The clinical route of application of cricket extract (Example) was oral, so the route of administration was oral, and TCDD was administered intraperitoneally.

Dosing method

Cricket extract (Example) was administered orally once daily for 1 day from the day after group separation using sonde, and TCDD was intraperitoneally using a 23-gauge syringe the day after the cricket extract was finished. Administered into.

Test Methods

Trial period: 28 days (14 days for cricket extract administration + 14 days for observation after TCDD administration)

The administration was orally administered to each dose for 14 days from the day after the group separation, saline in the negative control group (G1) and positive control group (G2), and cricket extract in the administration group (G3 to G5).

TCDD was administered with saline and cricket extracts for 14 days, followed by dissolving TCDD (2 mg) in a trace amount of dimethyl sulfoxide (DMSO) (50 μl) and a small amount of acetone (450 μl), followed by corn oil ( 4.5 ml) was used as a stock solution (400 µg / ml), and the working solution was diluted with corn oil so that the final concentration of TCDD was 60 µg / ml and then intraperitoneally administered. After the administration of TCDD, no cricket extract was administered for 14 days, only observations were made.

General symptoms observation

General symptoms were observed once a day during the cricket extract administration period, and confirmed whether death occurred twice a day during the observation period.

Weight measurement

Body weights of the test animals used for the test were measured twice a week after the start of cricket extract administration and immediately before necropsy during the preliminary group separation. Fasting was performed for about 18 hours immediately before autopsy.

Blood biochemical test

Blood biochemical tests showed that the animals obtained by fasting for 18 hours at necropsy were pre-bleeded from the abdominal aorta under ether anesthesia for 30 minutes at room temperature to coagulate, and then alanine the serum obtained by centrifugation (3,000 rpm, 15 minutes). transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), glucose, creatinine, total protein, albumin, uric acid, blood The urea nitrogen (BUN), calcium, and inorganic phosphate values were measured using an automatic analyzer (Hitachi-747, Hitachi Medical Co., Ltd., Japan).

Long-term weight measurement

After completion of the test, all test animals were treated with liver, heart, spleen, kidney (left and right), testicles (left and right), epididymis (left and right), prostate, lung, thymus, adrenal gland (left and right) and thyroid gland ( Left, right), submandibular glands (left, right) and brain organ weights were measured.

Histopathology

Livers were harvested and fixed in 10% neutral formalin. After sufficient fixation, the liver tissue was embedded in a paraffin embeder (Leica EG 116, Germany) and made into 4 µl sections using a rotary microtome (Leica 820, Sweden) and stained with hematoxylin and Eosin under an optical microscope. Inspected.

Statistical processing of data

The experimental results for weight change, organ weight, and serum biochemical test of the control group and the experimental group were expressed as mean ± standard deviation, and statistical significance was determined using Student's t-test method.

Results and Discussion

General symptoms observation

None of the animals died in all groups during the experimental period. No clinical symptoms were observed in all groups during the cricket extract, and during the observation period TCDD and TCDD were observed for the roughness and loss of body weight, especially in the TCDD alone group. Changes in appearance were noticeable.

Weight change

During the cricket extract administration, the body weight of all groups increased continuously, but during the 14-day observation period with TCDD, all groups except negative control group (G1) showed a continuous decrease in body weight after 7 days of TCDD administration. On the 14th day after TCDD administration, significant weight loss was observed in the TCDD alone group (G2; 317.78 ± 21.53g) compared to the body weight of the negative control group (361.74 ± 23.92g) (P <0.01), and cricket extract 50mg (G3). (Low dose group; 321.42 ± 35.59g and medium dose group: 325.30 ± 25.20g) in 100 mg (G4; medium dose group) (P <0.05). However, the cricket extract 200 mg (G5; high-dose group) showed a tendency to suppress this weight loss somewhat. This is shown in Figure 1 and Table 2.

division group G1 G2 G3 G4 G5 Days One 214.99 ± 4.53 214.39 ± 3.80 215.42 ± 4.68 214.25 ± 5.12 214.00 ± 4.98 3 230.79 ± 6.30 231.82 ± 6.69 235.38 ± 9.01 230.78 ± 8.80 231.95 ± 6.25 7 261.73 ± 9.08 263.35 ± 8.52 269.76 ± 10.85 260.40 ± 10.25 261.96 ± 12.09 10 283.12 ± 11.47 281.01 ± 7.90 286.81 ± 12.58 283.11 ± 6.77 280.94 ± 13.07 14 305.24 ± 15.68 319.85 ± 36.22 316.31 ± 15.11 306.38 ± 16.20 323.35 ± 33.41 17 321.24 ± 15.86 324.39 ± 12.23 336.56 ± 17.77 324.25 ± 19.34 328.38 ± 14.97 21 333.48 ± 18.54 330.82 ± 11.14 225.12 ± 21.77 327.92 ± 16.42 338.91 ± 20.88 24 347.52 ± 22.7 322.95 ± 14.26 * 326.11 ± 28.97 323.18 ± 17.78 * 337.42 ± 27.10 28 361.74 ± 23.92 317.78 ± 21.53 ** 321.42 ± 35.59 * 325.30 ± 25.20 * 339.28 ± 34.76  * Distinctly different from control (p <0.05) ** apparently different from control (p <0.01)

Blood biochemical test

AST activity of G2 group (744.46 ± 314.83IU / L) was significantly increased compared to G1 (P <0.01), but G3 group (257.74 ± 145.23IU / L) and G4 group (195.84 ± 145.23). IU / L) and G5 group (289.60 ± 110.72IU / L) significantly decreased compared to G2 group (P <0.01). In addition, the cricket extract was administered in G3 group (46.55 ± 13.73IU / L), G4 group (37.93 ± 6.24IU / L) and G5 group (39.75 ± 6.35IU / L) in ALT activity. L) showed a tendency to decrease significantly (G3 and G5 group; P <0.05, G4 group; P <0.01). This is shown in Figure 2 and Table 3.

G1 group G2 group G3 group G4 group G5 group enzyme AST (IU / L) 168.77 ± 15.01 744.46 ± 314.83 ** 257.74 ± 145.23 ## 195.84 ± 44.15 ## 289.6 ± 110.72 * ## ALT (IU / L) 57.63 ± 10.13 75.63 ± 26.59 46.55 ± 13.73 # 37.93 ± 6.24 ** ## 39.75 ± 6.35 ** # ALP (IU / L) 116.86 ± 15.77 104.43 ± 21.35 106.86 ± 36.05 94.43 ± 29.31 81.57 ± 13.94 ** # Glucose (mg / dl) 143.29 ± 27.45 123.71 ± 18.76 123.00 ± 12.69 128.86 ± 18.62 120.86 ± 8.801 Cholesterol (mg / ㎗) 64.57 ± 8.00 93.86 ± 20.75 ** 110.86 ± 32.78 ** 81.86 ± 16.15 * 89.43 ± 33.93 Triglycerides (mg / dl) 45.14 ± 16.38 50.29 ± 7.32 83.14 ± 10.95 ** ## 58.86 ± 10.01 74.57 ± 40.94 HDL (mg / dl) 44.83 ± 2.88 150.31 ± 248.68 66.63 ± 15.46 * 54.73 ± 8.98 * 54.11 ± 15.64 LDL (mg / dl) 10.11 ± 3.77 17.14 ± 5.19 * 19.81 ± 11.20 11.23 ± 2.83 # 12.20 ± 9.36 Creatinine (mg / dl) 0.52 ± 0.01 0.59 ± 0.05 * 0.55 ± 0.04 0.56 ± 0.03 ** 0.52 ± 0.03 # Total protein (g / ㎗) 6.32 ± 0.31 6.79 ± 0.42 * 6.78 ± 0.41 * 6.99 ± 0.20 ** 6.67 ± 0.21 * Albumin (g / ㎗) 3.94 ± 0.13 4.29 ± 0.15 ** 4.22 ± 0.23 * 4.38 ± 0.16 ** 4.19 ± 0.12 ** Uric acid (mg / dl) 0.55 ± 0.22 0.32 ± 0.33 0.35 ± 0.33 0.16 ± 0.12 ** 0.28 ± 0.33 BUN (mg / ㎗) 16.00 ± 1.73 25.014 ± 3.34 ** 24.29 ± 3.55 ** 23.29 ± 3.90 ** 22.43 ± 3.41 ** Calcium (mg / ㎗) 10.96 ± 0.32 13.73 ± 1.11 ** 13.53 ± 2.05 * 12.50 ± 0.77 ** # 13.44 ± 0.84 ** Inorganic phosphorus (mg / dl) 7.53 ± 0.52 8.22 ± 0.83 7.57 ± 1.17 7.33 ± 0.42 # 7.12 ± 0.81 #  * Clearly different from control group (p <0.05) ** Clearly different from control group (p <0.01) # Clearly different from group G2 (p <0.05) ## Clearly different from group G2 (p <0.01)

Change in relative organ weight

Changes in the relative organ weight of the liver were negative in the G2 group (4.56 ± 0.37%), G3 group (5.01 ± 0.36%), G4 group 4.44 ± 0.17%) and G5 group (4.36 ± 0.76%). 0.14%) showed a tendency to increase significantly (P <0.01). However, this increase in organ weight tended to decrease somewhat in the G5 group. This is shown in Figure 3 and Table 4.

G1 group G2 group G3 group G4 group G5 group Agency liver 2.75 ± 0.14 4.56 ± 0.37 ** 5.01 ± 0.36 ** 4.74 ± 0.17 ** 4.36 ± 0.76 ** spleen 0.23 ± 0.03 0.25 ± 0.03 0.25 ± 0.05 0.25 ± 0.05 0.26 ± 0.04 Height (left) 0.37 ± 0.03 0.38 ± 0.03 0.41 ± 0.05 0.40 ± 0.04 0.40 ± 0.03 Xinjiang (right) 0.36 ± 0.04 0.39 ± 0.05 0.43 ± 0.05 * 0.39 ± 0.03 0.40 ± 0.03 * Testicle (left) 0.47 ± 0.08 0.59 ± 0.08 * 0.56 ± 0.04 * 0.57 ± 0.06 * 0.53 ± 0.05 Testicle (right) 0.49 ± 0.07 0.57 ± 0.07 0.55 ± 0.04 0.59 ± 0.07 * 0.53 ± 0.04 Epididymis (left) 0.14 ± 0.04 0.16 ± 0.03 0.16 ± 0.04 0.16 ± 0.02 0.15 ± 0.02 Epididymis (right) 0.16 ± 0.03 0.17 ± 0.04 0.16 ± 0.02 0.15 ± 0.02 0.16 ± 0.02 prostate 0.17 ± 0.06 0.15 ± 0.06 0.09 ± 0.04 * # 0.12 ± 0.07 0.12 ± 0.04 Heart 0.34 ± 0.03 0.35 ± 0.03 0.38 ± 0.06 0.37 ± 0.10 0.34 ± 0.01 lungs 0.49 ± 0.08 0.51 ± 0.06 0.57 ± 0.07 * 0.45 ± 0.09 0.52 ± 0.04 Thymus 0.19 ± 0.02 0.07 ± 0.05 ** 0.04 ± 0.01 ** 0.04 ± 0.01 ** 0.06 ± 0.05 ** Adrenal gland (left) 0.008 ± 0 0.010 ± 0 0.012 ± 0.01 0.008 ± 0 0.008 ± 0 Adrenal gland (right) 0.008 ± 0 0.009 ± 0 0.011 ± 0.01 0.009 ± 0 0.009 ± 0 Thyroid gland (left) 0.003 ± 0 0.007 ± 0.01 0.004 ± 0 0.003 ± 0 0.004 ± 0 Thyroid gland (right) 0.002 ± 0 0.002 ± 0 0.004 ± 0 0.003 ± 0 0.004 ± 0 * # Mandibular gland 0.22 ± 0.05 0.18 ± 0.03 0.18 ± 0.03 0.17 ± 0.03 0.17 ± 0.03 brain 0.60 ± 0.04 0.70 ± 0.08 * 0.69 ± 0.07 * 0.65 ± 0.06 0.64 ± 0.06  * Distinctly different from control group (p <0.05) ** apparently different from control group (p <0.01) # apparently different from G2 group (p <0.05)

Histopathology

In histopathological examination of the liver, G2 group was observed around the portal triad, and inflammatory cell infiltrations were observed. Infiltrate into focal phases. Severe vacuolations were also observed throughout the lobules. However, these lesions showed a tendency to be inhibited by the administration of cricket extracts, and especially in the G5 group administered with high concentration of cricket extracts, the infiltration and phobia of inflammatory cells tended to be markedly reduced.

TCDD causes hepatic hypertrophy in all animal species used to date, due to hypertrophy of SER caused by TCDD affecting the cytochrome P-450 enzyme system It is known to be due to hyperplasia and hypertrophy of parenchymal cells. In addition, when TCDD damages the liver, the permeability of the cell membrane due to the destruction or inflammation of the liver cells is enhanced, and intracellular substances including enzymes such as ALT and AST are leaked into the blood to increase blood concentration and activity of the enzymes. In the experiments of the present invention, the administration of TCDD also resulted in such an increase in hepatic hypertrophy and thus an increase in relative organ weight, and an increase in blood enzyme activity. This tendency is improved by administration of the cricket extract according to the present invention. Indicated. In addition, the histologic examination showed a tendency of inhibiting hepatocyte damage due to the administration of cricket extract in the G3 group to the G5 group compared to the G2 group, especially in the G5 group. Figure 4 in the embodiments of the present invention stained liver tissue of rats of the G1 group (group orally administered to rats only saline containing no cricket extract without TCDD treatment) with hematoxylin / eosin staining reagent and 100 times 4 (A) shows the liver defibrillation and FIG. 4 (B) shows the central artery part. It was confirmed that no damage was observed.

Figure 5 in the embodiments of the present invention the liver tissue of the G2 group (oral administration of physiological saline not containing the cricket extract, and then injected with TCDD) stained with hematoxylin / eosin staining reagent 5 (A) is an enlarged photograph of 100 times, and FIG. 5 (B) is an enlarged photograph of 200 times. In FIG. 5, it was confirmed that severe phobias (black arrows) and inflammatory cell infiltration (blue arrows) were observed.

Figure 6 in the embodiments of the present invention the liver tissue of the G4 group (Cricket extract 100mg / kg orally administered to the rat, then injected with TCDD) mice stained with hematoxylin / eosin staining reagent and 100 6 (A) shows the liver defibrillation and FIG. 6 (B) shows the central artery part. A slight degree of phobia and inflammatory cell infiltration were observed.

Figure 7 in the embodiments of the present invention the liver tissue of rats of the G5 group (Cricket extract 200mg / kg orally administered to the rat, then injected with TCDD) mice stained with hematoxylin / eosin staining reagent 100 7 (A) shows liver defibrillation and FIG. 7 (B) shows a central artery part. Severe phobia and inflammatory cell infiltration were normalized by cricket extract.

Therefore, according to the present invention, the cricket extract can effectively prevent the hepatotoxicity of TCDD, a representative environmental toxic substance, through various actions such as inhibition of weight loss, improvement of enzyme levels in blood, inhibition of hepatic hypertrophy and consequent hepatocyte protection. In particular, the effective concentration was confirmed to be represented by more than 200mg / ㎏.

Therefore, according to the present invention, there is an effect of providing a food comprising a cricket extract having a prophylactic effect against hepatotoxicity caused by a toxic compound such as dioxins.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (3)

500 g of dried adults of subtropical crickets of the genus Gryllus bimaculatus were hydrothermally extracted in 4,000 to 6,000 ml of distilled water, and the obtained hot water extract was concentrated at a ratio of 1/50 to 1/55, and then lyophilized. Food comprising a cricket extract having a preventive effect on hepatotoxicity, characterized in that the extract obtained by making an effective concentration comprising at least 200 mg / kg. delete delete

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