KR101712557B1 - Cosmetic composition including a ceriporia lacerata and green tea and cosmetics using the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising: a mixed extract composed by mixing a Ceriporia lacerata extract and a green tea extract; a fermented product obtained by aging and fermenting the mixed extract; powder produced by lyophilizing the mixed extract and crushing the same; or a fermented product by fermenting the Ceriporia lacerata extract, and to cosmetics composed of a part with respect to the total weight of the cosmetic composition.

Description

Technical Field [0001] The present invention relates to a cosmetic composition using cerolaria lacerata and green tea,

The present invention relates to a mixed extract obtained by mixing a granulocyte lucerata extract and a green tea extract, a fermented product obtained by aging and fermenting the mixed extract, a powder prepared by lyophilizing the mixed extract, A cosmetic comprising a fermented product itself obtained by fermenting a cerata extract, and a cosmetic comprising a part of the above cosmetic composition relative to the total weight.

Environmental pollution caused by rapid industrialization and the life of complex metropolitan cities have a great influence on the patterns of consumer life of modern people. One of the changes of living patterns is a well-being trend to increase life satisfaction.

This well-being trend is a phenomenon that promotes naturalism in addition to organic foods. In the cosmetics industry, it also minimizes the use of artificial ingredients and continues to show naturalistic cosmetics and cosmetics for extracting natural ingredients that protect and improve skin through natural ingredients. , Thereby gaining explosive popularity among consumers.

In accordance with the above trend, the present invention provides a cosmetic composition made of a natural material having superior functionality to consumers and a cosmetic using such a cosmetic composition.

In particular, the present invention provides a cosmetic composition comprising a main ingredient of an extract of Ceriporia lacerata, which is known to have diabetes-preventing function, and cosmetics containing the cosmetic composition in a predetermined amount.

Ceriporia lacerata is a kind of porridge and mushroom, which is a by-product of oak and deer. Traditionally, Ceriporia lacerata has been used for medical purposes in China, Japan and Korea, or has been used as natural bioremediation due to lignin degradation.

As a technique using such a Ceriporia lacerata, the present applicant has been registered with the Korean Intellectual Property Office as a patent application (Application No. 10-2015-0027093) on Feb. 26, 2015 Patent No. 10-1555415, filed on May 15, 2015, entitled Cosmetic Composition Containing Extract of Seripolya Lacerata and Cosmetic Products Using the Same)

The above-mentioned patent discloses an invention for providing a cosmetic composition which is prepared by using Ceriporia lacerata extract alone or by further adding ginseng fruit extract and grape seed extract to the above-mentioned Sepiolaria lacerata extract to be.

The present applicant has accomplished the present invention by further improving the technique disclosed in the above-mentioned conventional patent to improve the technique for providing high-function cosmetics.

Korean Registered Patent No. 10-1522415 (registered on May 15, 2015)

The present invention relates to a method for producing a fermented product, which comprises a mixed extract comprising a mixture of Ceriporia lacerata extract and green tea extract, a fermented product obtained by aging and fermenting the mixed extract, a powder prepared by lyophilizing the mixed extract, It is an object of the present invention to provide a cosmetic comprising a fermented product itself obtained by fermenting an extract of Sellapora lacerata and a cosmetic comprising a part of the above cosmetic composition in its total weight.

In order to achieve the above object,

The present invention relates to a method for preparing a granular starch, which comprises washing a granulia lacerata with drinking water two to three times, drying it with a freeze drier, pulverizing the freeze-dried granulia lacerata with a crusher, The mixture was stored at 15 ~ -25 ° C for 3 ~ 5 days at room temperature, added with 80% methanol (MeOH) in a cryoprotectant. The extract was subjected to primary filtration, centrifugation at 3000 x g for 2 to 4 minutes, extraction of the supernatant, filtration of the filtrate with a 0.1-0.3 μm filter, 1 to 99 wt%

Green tea powder and ethanol (EtOH) were weighed in a homogenizer at a weight ratio of 1:20 to 30, and the extract was filtered while being ground and filtered by a glass filter. The filtrate was concentrated under reduced pressure using a vacuum concentrator to remove ethanol, And 1 to 99 wt% of the total weight of the cosmetic composition of the present invention.

The cosmetic composition and the cosmetic composition containing the same according to the present invention can be used as a cosmetic composition containing three lipolylacerases which are excellent in whitening effect, antioxidant activity, skin irritation degree, moisturizing effect, skin elasticity, , An antioxidative effect for suppressing the production of active oxygen which is a main cause of skin cancer or skin aging caused by ultraviolet irradiation, a cosmetic composition effective for skin care and health using green tea having an effect of preventing and inhibiting skin aging due to ultraviolet rays, And has an effect of providing cosmetics.

Hereinafter, a detailed description will be given of the above technical constitution.

As described above, the cosmetic composition using the ceriplora lacerata and green tea according to the present invention may be prepared by mixing a green tea extract and a green tea extract; Or a mixture of green tea extract and freeze-dried mixture of green tea extract and green tea extract; Or by fermenting a mixture of an extract of Celporia lacerata and a green tea extract; Or by fermenting the extract of Sera lipolactacerata itself.

As described above, Ceriporia lacerata is a kind of porridge and mushroom which is a by-product of oak or rape. It is used for medical treatment purposes in China, Japan and Korea, or has a lignin-decomposing action, And has a whitening effect, an antioxidant activity, a skin irritation degree, a moisturizing effect, a skin elasticity, and a skin regeneration function.

In addition, the green tea contains a large amount of catechin ingredient, so that it can act to converge and soothe tired skin and prevent aging of the skin. In addition, it exhibits an antioxidative effect that suppresses the production of active oxygen, which is a main cause of skin cancer and skin aging caused by ultraviolet irradiation, and thus has action to prevent and inhibit skin aging caused by ultraviolet rays, and has an effect of suppressing erythema . Vitamin A, Vitamin C, and Vitamin E are the other ingredients that are good for skin beauty that exists in green tea.

The vitamin A has a function of keeping skin cells and mucous cells in a healthy state, and vitamin C acts to prevent deposition of melanin pigment and to suppress the formation of spots and freckles and to keep the skin whitened. Vitamin E is a typical antioxidant and is very important for maintaining normal skin condition. It is effective for seborrheic, dry, irritable, acneous skin, and makes blood circulation smooth and maintains normal blood color.

In the case of cosmetics using fermented products, antioxidants that reduce free radicals, which are the main cause of wrinkles and aging, and cell functions of skin, collagen synthesis, whitening, moisturizing, etc., It is possible to further enhance the functionality of the cosmetic composition and the cosmetic composition containing the green tea according to the present invention.

More specifically,

As a first embodiment, a three-lipolylacerase is washed with drinking water two or three times, dried with a freeze dryer, lyophilized three-lipoa lactase is pulverized using a crusher, and then dropped into 80 to 120 mesh sieve The powder was stored frozen at -15 to -25 ° C and stored in a freezer and stored in 80% methanol (MeOH). The powder was added at a ratio of 1:20 to 30, and the mixture was extracted at room temperature for 3 to 5 days , And the extracted extract is subjected to primary filtration and centrifugation at 3000 x g for 2 to 4 minutes. The supernatant is taken out, and 1 to 99% by weight of an extract of Sellapora lacerata, which is filtered with a filter of 0.1 to 0.3 탆,

Green tea powder and ethanol (EtOH) were weighed in a homogenizer at a weight ratio of 1:20 to 30, and the extract was filtered while being ground and filtered by a glass filter. The filtrate was concentrated under reduced pressure using a vacuum concentrator to remove ethanol, 1 to 99 wt.% Of a mixture of Celpolyac Lactacrata and Green tea mixture is used as a cosmetic.

As a second embodiment, a powder prepared by freeze-drying, rather than using the mixed extract of ceriplora lactaclora and green tea as it is,

In 85 to 95 wt% of a protective agent mixed with skim milk and sucrose at a weight ratio of 2.5 to 3.5: 1,

5 to 15 wt% of the mixed extract of Sellapora lacera and green tea is added and lyophilized, followed by pulverization using a crusher, and then powder reduced to 80 to 120 mesh sieve is used as a cosmetic.

As a third embodiment, a fermented mixture of ceriplora lactaclora and green tea is used as a cosmetic,

A fermented product obtained by fermenting the mixed extract of Sellapora lacera and green tea for 5 to 90 days in a sealed state is used as a cosmetic product.

As a fourth embodiment, a fermented product obtained by fermenting the above-mentioned extract of Sera lipolacrata in a sealed state for 5 to 90 days is used as a cosmetic product, using the Sera lipolactarata extract described in Example 1 alone.

Also, the cosmetic according to the present invention comprises a certain amount of the cosmetic composition obtained from the first to fourth embodiments, and more specifically, it is prepared by adding 0.1 to 50 wt% to the total weight of the cosmetic.

At this time, cosmetics can be variously selected from basic cosmetics, makeup cosmetics, hair cosmetics, perfumes, and more specifically, cosmetics, essence, hair tonic, hair liquid, perfume, cream, lotion, mascara and foundation.

The components of the cosmetics are largely divided into Aqueous and Oil phaes. The aqueous component is soluble in water and the oily component is an oil-soluble component. Typical examples of water-based ingredients include glycerin, which is used as a humectant, and oil-based components, such as oil and wax. These are basically used in most cosmetics. As a result, cosmetics are composed of an aqueous component and an oily component properly mixed, and include purified water, oily ingredients, a surfactant, a moisturizer, a perfume, and a functional ingredient.

The purified water accounts for more than 70% of the cosmetics. In the past, it was merely a base for making cosmetics. However, by using water such as volcanic rocks, tree sap, ice water, and floral water, have.

The oil-based raw material is used for preventing evaporation of water to prevent drying, softening the feel of the skin, and improving the feeling of use. Animal oil; Mineral oil; Synthetic oils such as isopropyl myristate, 2-oxydodecyl myristate, and the like; Waxes such as beeswax, candelilla wax and jojoba oil; Cetyl alcohol, and higher alcohol such as lauryl alcohol.

The surfactants are collectively referred to as materials having structural characteristics that have a hydrophilic group and a hydrophilic group in one molecule in chemical structure. Surfactants have ionic properties on one side and they dissolve in water and on the other side they are like hydrocarbons and dissolve in oil.

The moisturizer may be healthy and resilient if it has enough moisture on the skin. By using a moisturizing agent, moisture can be put on our skin or hair to prevent drying. Examples of the moisturizing agent used in cosmetics include polyhydric alcohols such as glycerin, propylene glycol, and polyethylene glycol; Natural moisturizing factors such as sodium lactate, sodiphene PCA, and sodium hyaluronate.

The colorant may be incorporated in cosmetics to express color, have coating power, or reflect ultraviolet light. Some makeup products, such as foundation and eye shadow, are used for color, while some basic cosmetics are used for visual effects. Controversy has arisen as a cause of human harmful factors such as carcinogenicity and contact dermatitis. Representative ingredients include tar pigment, natural pigment, and inorganic pigment.

The polymeric substance refers to a large compound having a large molecular weight and is used in some applications as a thickener, a film forming agent, a moisturizing agent, or a surfactant depending on the application. Typical examples are Quince seed gum, Xanthan gum, and carboxyl vinyl polymer.

The fragrance can be used to increase the value of cosmetics by giving good fragrance to cosmetics and to eliminate the odor peculiar to the raw materials. Nowadays, fragrance therapy called "aromatherapy" is introduced, and by using herbal scent in cosmetics, it is trying to give mental stability and comfort to modern people suffering from stress and various diseases. However, fragrances also vary from person to person and there are many side effects, so proper use ratio should be taken into consideration when mixing in cosmetics.

However, the antidiabetic effect and mechanism by Ceriporia lacerata is not known at present. In this study, we investigated the cytotoxicity (reduction of survival rate, induction of cell death) and mechanism of Dex administration in rat pancreatic beta cell line (INS-1), and at the same time, the extract of Ceriporia lacerata extract, CLE ) To Dex-induced INS-1 cytotoxicity. In addition, we evaluated the effect of Dex on the induction of steroid diabetes and the effect and mechanism of Dex induced diabetes inhibition by CLE in Wistarrat.

Preservatives: When you use cosmetics, cosmetics may be deteriorated by contamination with microorganisms. When cosmetics change, there is a problem with stability, but it can cause skin diseases by microorganisms, which also causes safety problems. In cosmetics, it is preferable to use a mixture of various kinds of preservatives rather than a single preservative in terms of efficacy, so it is preferable to mix them. However, preservatives have a controversial safety that adversely affects the human body. Representative components include parabens, imidazolidinyl urea, phenoxyethanol, and the like.

Hereinafter, the technical composition of the cosmetic composition according to the present invention and the cosmetic composition containing the cosmetic composition will be described in detail with reference to examples.

Three Li Pori Lacerata  Extract and green tea extract By mixture  The resulting mixture of extracts Cosmetics  Composition

Example 1 is intended to use the mixed extract itself as a cosmetic composition, which is a mixture of an extract of Sellapora lacerata and a green tea extract.

for teeth,

The granules were washed three times with drinking water and then dried with a freeze dryer. The lyophilized granules were crushed using a crusher, and the powder obtained by lowering the granules in a 100 mesh sieve was frozen at -20 ° C, The cryoprecipitate was added to 80% methanol (MeOH) at a weight ratio of 1:20. After 4 days of extraction at room temperature, the extracted extract was firstly filtered and centrifuged at 3000 × g for 3 minutes , 30% by weight of an extract of Sellapora lacerata obtained by filtering only the supernatant with a 0.2 탆 filter,

Green tea powder and ethanol (EtOH) were weighed in a homogenizer at a weight ratio of 1:20 to 30, and the extract was filtered while being ground and filtered by a glass filter. The filtrate was concentrated under reduced pressure using a vacuum concentrator to remove ethanol, And 70 wt% of the total weight of the mixture.

A cosmetic composition comprising a fine powder of a mixed extract of Sellapora lacera and green tea

The present Example 2 is intended to use the fine powder obtained by freeze-drying the mixed extract of ceriplora lactaclora and green tea of Example 1 and powdering it as a cosmetic composition.

for teeth,

To 90 wt% of a protective agent mixed with skim milk and sucrose at a weight ratio of 3: 1,

10% by weight of the mixed extract of Sellapora lacera and green tea is added, and the mixture is lyophilized, pulverized using a crusher, and then dropped in a 100 mesh sieve, to give a powder of a mixed extract of Sellapora lacera and green tea.

Three Li Pori Lacerata · Green tea mixed extract With fermented water  Done Cosmetics  Composition

In Example 3, the fermented product itself obtained by aging and fermenting the mixed extract of Sellapora lacera and green tea is used as a cosmetic composition.

for teeth,

The fermented product obtained by fermenting the mixed extract of ceriplora lacera and green tea shown in Example 1 in a sealed state at room temperature for 10 days is used as a cosmetic composition.

Three Li Pori Rock Serrat With fermented water  Done Cosmetics  Composition

Example 4 is intended to use the fermented product itself obtained by fermenting ceriplora lucerata as a cosmetic composition.

for teeth,

A fermented product obtained by fermenting the cerporolia lactase shown in Example 1 in a sealed state at room temperature for 10 days is used as a cosmetic composition.

Example  1 of Cosmetics  Cosmetics manufactured using the composition

2.5 g of lipophilic monostearic acid glycerin; Butyleneglycol 2.0 g; 2.2 g stearic acid; 1.0g wax; Polysorbate 60 1.5 g; 0.6 g of sorbitan stearate; 1.0 g of hydrogenated vegetable oil; Squalane 3.0g; 5.5 g mineral oil; 5.5 g of trioctanoin; Dimethicone 1.0 g; 0.1 g of sodium magnesium silicate; 5.5 g of glycerin; Betaine 3.0 g; 1.0 g of triethanolamine; 4.0 g of sodium hyaruronate; Preservative, fragrance, pigment 0.01g; 45.59 g of purified water was firstly stirred at 4,000 rpm for 8 minutes, then stirred at 5,000 rpm for 4 minutes and then cooled.

After cooling, 15 g of the cosmetic composition of Example 1 was added, and the mixture was stirred for 3 minutes at 4,800 rpm for 10 minutes to cool the nutritional cream.

Example  2 of Cosmetics  Cosmetics manufactured using the composition

The nutritional cream was produced in the same composition and the same mixing ratio as in Example 5, except that the cosmetic composition prepared in Example 2 was used in selecting the cosmetic composition.

Example  3 of Cosmetics  Cosmetics manufactured using the composition

The nutritional cream is produced in the same composition and the same ingredient ratio as in Example 5, but the cosmetic composition prepared in Example 3 is used in selecting the cosmetic composition.

Example  4 of Cosmetics  Cosmetics manufactured using the composition

The nutritional cream is produced in the same composition and the same ingredient ratio as in Example 5, but the cosmetic composition prepared in Example 4 is used in selecting the cosmetic composition.

[ Comparative Example  One]

Comparative Example 1 is a nutrition cream not containing the cosmetic composition according to the present invention,

3.5 wt% of lipophilic monostearic acid glycerin; 4.0 wt% of stearyl alcohol; 3.2 wt% stearic acid; Wax 2.0wt%; Polysorbate 60 2.5 wt%; 1.6 wt% sorbitan stearate; 2.0 wt% of hydrogenated vegetable oil; Squalane 4.0wt%; Mineral oil 5.5wt%; 5.5 wt% of trioctanoin; Dimethicone 1.0wt%; 1.1 wt% sodium magnesium silicate; 6.5 wt% glycerin; Betaine 4.0 wt%; 3.0 wt% of triethanolamine; 5.0 wt% sodium hyaruronate; Preservative, incense, pigment 0.01wt%; 45.59 wt% of purified water was stirred at 4,800 rpm for 10 minutes and cooled to complete the nutritional cream.

[ Test Example  One]

The whitening effect of the cosmetics prepared in Examples 5 to 8 and Comparative Example 1

After diluting the cosmetic samples of Examples 4 to 6 and Comparative Example 1 in 50% dimethyl sulfoxide (DMSO), 50 占 퐇 of each of the cosmetic samples was taken, and then 450 占 퐇 of 0.1M phosphate buffer, tyrosinase (sigma ).

60 μl of 1.5 mM trypsin (Sigma) was added to the mixed solution, vortexed for 10 seconds, left at 40 ° C for 20 minutes, and absorbance was measured at 490 nm relative to the control (Arbutin).

As a result of measuring the whitening activity at 40 캜,

Example 5 was 31.12%, Example 6 was 29.54%, Example 7 was 32.20%, Example 8 was 28.72%, and Comparative Example 1 was 13.15%. As a result, the whitening activity of Example 7 showing the cosmetic using the fermented product obtained by fermenting a mixture of the extract of Celpolia lacerata and green tea was the highest.

[ Test Example  2]

The antioxidant activity of the cosmetics prepared in Examples 5 to 8 and Comparative Example 1

After diluting the cosmetic samples of Examples 4 to 6 and Comparative Example 1 with 50% dimethylsulfoxide (DMSO), 50 占 퐇 of each cosmetic sample was taken, and 10 mM DPPH (1,1-diphenyl-2 picrylhydrazyl) solution was added and vortexed for 10 seconds. Thereafter, 1 mL of distilled water was added, and the solution was left at 40 DEG C for 40 minutes, and the absorbance was measured at 517 nm as compared with the comparative example.

As a result of measuring the antioxidative activity at 40 캜,

Example 5 was 53.15%, Example 6 was 52.25%, Example 7 was 59.25%, Example 8 was 52.55%, and Comparative Example 1 was 12.18%. The antioxidative effect was the highest in Example 7, in which a cosmetic using a fermented product obtained by fermenting a mixture of the extract of Sellapora lacerata and green tea was presented.

[ Test Example  3]

The skin irritation degree of the cosmetics manufactured in Examples 5 to 8 and Comparative Example 1

Skin tests were conducted to examine the degree of skin irritation of the cosmetics manufactured in Examples 5 to 8 and Comparative Example 1.

Fifty adults were selected for skin test. The age range is from 25 to 60 years, with 20 males and 30 females.

The subjects were divided into five groups of 10 persons. The first group contained the cosmetics of Example 5, the second group contained the cosmetics of Example 6, the third group contained the cosmetics of Example 7, and the fourth group contained the cosmetics of Example 8 And the cosmetic sample of Comparative Example 1 was applied to the upper half of the arm, and after 1 hour, the skin reaction was visually inspected for the first time, and after 24 hours, the second visual inspection was performed again. Respectively.

Skin irritation division Average score Example 5 ○ Example 6 ○ Example 7 ○ Example 8 ○ Comparative Example 1 ○

(?: No skin change,?: Mild erythema, x: marked erythema)

[ Test Example  4]

The moisturizing effect of the cosmetics prepared in Examples 5 to 8 and Comparative Example 1

To confirm the moisturizing effect of Examples 5 to 8 and Comparative Example 1, 50 male and female adults were selected. The age range is from 25 to 60 years, with 20 males and 30 females.

The subjects were divided into five groups of 10 persons. The first group contained the cosmetics of Example 5, the second group contained the cosmetics of Example 6, the third group contained the cosmetics of Example 7, and the fourth group contained the cosmetics of Example 8 And the moisturizing effect of the skin was evaluated by the 5-point scaling method using the cosmetics of Comparative Example 1 and the 5th group. The results are shown in Table 2 below.

Moisturizing effect division Average score Example 5 4.5 [(5 points * 5) + (4 points * 5) / 10] Example 6 4.4 [(5 points * 5) + (4 points * 4) + (3 points + 1) / 10] Example 7 4.7 [(5 points * 7) + (4 points * 3) / 10] Example 8 4.0 [(5 points * 2) + (4 points * 6) + (3 points * 2) / 10] Comparative Example 1 2.5 [(3 points * 5) + (2 points * 5) / 10]

(1: very bad, 2: bad, 3: normal, 4: good, 5: very good)

As can be seen from the results of Table 2, the cosmetic according to the present invention is remarkably effective in moisturizing effect as compared with Comparative Example 1 which is a typical cosmetic product. It was confirmed that the cosmetic using the fermented product obtained by fermenting the mixture of the extract of Cerporaria lacera and Green tea (Example 8) exhibited the highest moisturizing effect.

The cosmetic composition and the cosmetic composition containing the same according to the present invention have whitening effect, antioxidant activity, skin irritation degree and moisturizing effect, and also have high quality and high functionality The provision of cosmetics has great potential for industrial applications.

Claims (5)

The granules were washed with drinking water for 2 ~ 3 times, dried with a freeze drier, and lyophilized granules were crushed using a crusher. The powder was dropped into 80 ~ 120mesh sieve, The mixture was added to 80% methanol (MeOH) at a ratio of 1:20 to 30, and the mixture was extracted at room temperature for 3 to 5 days. The extracted extract was dissolved in 1 Centrifuging at 3000 x g for 2 to 4 minutes, filtering the supernatant with a filter of 0.1 to 0.3 mu m to obtain a cosmetic composition comprising a saponin extract,

Wherein the cosmetic composition comprises 1 to 99% by weight of the above-mentioned triglycerolactera extract; Green tea powder and ethanol (EtOH) were weighed in a homogenizer at a weight ratio of 1:20 to 30, and the mixture was subjected to grinding, followed by filtration through a glass filter. The filtrate was concentrated under reduced pressure using a vacuum concentrator to remove ethanol, 1 to 99 wt%; 5 to 15% by weight of the 100% by weight mixture of Seripolya lactacerata and green tea mixture prepared by mixing and sealing for 5 to 90 days in a sealed state,
The powder is added to 85 to 95 wt% of a protective agent mixed with skim milk and sucrose at a weight ratio of 2.5 to 3.5: 1, lyophilized, pulverized with a crusher, and dropped into 80 to 120 mesh sieve. Composition of cosmetic using Lia Laceratta and green tea.
delete delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is prepared by adding 0.1 to 50 wt% of a cosmetic composition comprising cerophora lucerata and green tea according to the total weight of the cosmetic.