KR101658061B1 - A cosmetic composition comprising of oriental medicinal herbs extracts fermented - Google Patents

Abstract

The present invention relates to a cosmetic composition or food composition for preventing or treating antioxidant or antioxidation, which comprises a fermented extract of oriental herbal medicinal herb complex, herbal composition, killing herb, and herb composition. The present invention also relates to a cosmetic composition for improving wrinkles comprising an oriental medicinal herb complex fermented extract as a active ingredient, According to the present invention, the herbal composition, herbal composition, killing herb, and herb complex herbal composition fermented extract have an antioxidative effect with excellent DPPH radical scavenging activity and NO inhibitory activity, and exhibit remarkably excellent antioxidant effect. In addition, the herbal composition complex fermented extract has an excellent effect of inhibiting the activity of elastase and collagenase and has an effect of improving wrinkles. Therefore, according to the present invention, the herbal extract, herbal medicine, herbal medicine, herbal medicine and herbal medicine combined fermented extract can be used as a functional raw material useful for antioxidation, anti-aging and anti-wrinkle effect.

Description

[0001] The present invention relates to a cosmetic composition comprising an oriental medicinal herbal extracts fermented extract as an active ingredient,

The present invention relates to a cosmetic composition or food composition for preventing or treating antioxidant or antioxidation, which comprises a fermented extract of oriental herbal medicinal herb complex, herbal composition, killing herb, and herb composition.

The present invention also relates to a cosmetic composition for improving wrinkles comprising an oriental medicinal herb complex fermented extract as a active ingredient,

The human body is in close contact with the external environment and is easily sensitized by environmental pollution, harmful substances, stress and irregular lifestyle. Skin exposed to this external environment is exposed to free radicals (free radicals), and the generated free radicals and free radicals attack normal tissue or cell membranes, destroying the cells, and the skin eventually becomes inflamed do. Inflammatory tissue is a major cause of atopic dermatitis and skin aging as well as skin troubles by destroying proteins and genetic material by physiological mechanism of skin. In addition, when the skin is exposed to ultraviolet light, the action of tyrosinase promotes melanin synthesis in the melanosome, thereby accelerating the aging of the skin. In this case, the generated free radicals may cause damage to cells or organelles, It causes oxidation of amino acid and degradation of protein. It also damages the nucleic acid, which can cause mutation or cancer by causing deformation of the nucleotide base, liberation of the nucleotide base, cleavage of the bond, and oxidative degradation of the sugar.

Elastase is known to be the only degradative enzyme that degrades elastin, an insoluble elastic fiber protein in animal connective tissue that maintains skin elasticity. It is also known as an enzyme that breaks down invading microorganisms or removes damaged cells to provide space for cell regeneration Function. Such an elastase is aged or excessively generated by external skin stimulation such as ultraviolet rays to excessively decompose elastin and weaken the collagen interlinkage, resulting in collapse of the collagen and elastin network structure in the dermis tissue, ≪ / RTI > Especially after the age of 40, the elasticity of the skin is rapidly reduced by the action of elastase, because elastase activity becomes active as the age increases, so that the elastin fibers partially disappear or coagulate and collagen fibers decrease. Therefore, it is possible to suppress the generation of wrinkles of the skin by inhibiting the activity of such elastase.

In the cosmetics manufacturing industry, efforts to suppress skin pigmentation abnormalities and skin aging due to appearance of skin damage due to external environment or internal mechanism are becoming a great concern. To this end, extracts of certain substances using natural substances Have been put into practice as a component of cosmetics, and studies on cosmetic compositions using herbal medicine extracts have been actively conducted.

On the other hand, Hwasungcho contains active ingredients in whole leaves, stems and roots, and physiologically active substances including distinctive odors help metabolism, cleanse blood, promote kidney function, excrete inflammation symptoms and toxins And is used in herbal medicine for hypertension, constipation, diabetes, fever, swelling, detoxification, arthritis, inflammatory diseases and skin diseases.

In addition, it is a type of green tea that is roasted by drying green tea. It is antioxidant, anticancer, blood lipid lowering effect in cardiovascular, prevention of atherosclerosis, accelerating blood circulation, Bacterial heterogeneity treatment and so on.

In addition, it is effective for the vomiting due to seawater and sputum due to the waste heat, the fever due to the fever, and the vomiting due to gastric emptying, .

In addition, the herbaceous gardens are used as a medicinal plant in the whole plant, except for roots. They have a fever and analgesic effect, are effective for cataracts, and are used as raw and tonic agents.

However, the antioxidant, antioxidant or wrinkle-reducing effect of herbal extracts of Oriental herb medicine, herbal medicine, killer, and herbal medicine herbal medicine has not been disclosed.

The inventor of the present invention has found that the herbal medicine combined extract of Sophora japonica, Cryptomeria japonica, Killer and Kidok is effective for antioxidation, anti-aging and wrinkle improvement while studying a novel cosmetic composition using the herbal medicine extract. .

It is an object of the present invention to provide a composition for preventing or antioxidizing antioxidant comprising an oriental herbal composition, a herbal composition, a killing herb and a herbal composition complex herbal extract as an active ingredient.

It is an object of the present invention to provide a composition for improving wrinkles containing an oriental herb complex, a herbal composition, a killing herb, and a herbal composition complex herbal extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for antioxidant or antioxidation, which comprises, as an active ingredient, a fermented extract of oriental herb medicine, horse mackerel, killer, and herb complex.

The present invention also provides an antioxidant or antioxidant food composition containing a fermented extract of oriental medicinal herb complex, herbal composition, herbal medicine, killing herb and gravel.

In addition, the present invention provides a cosmetic composition for improving wrinkles comprising an oriental herbal composition, a herbal composition, a killing herb, and a herbal composition complex herbal extract as an active ingredient.

According to the present invention, the herbal composition, herbal composition, killing herb, and herb complex herbal composition fermented extract have an antioxidative effect with excellent DPPH radical scavenging activity and NO inhibitory activity, and exhibit remarkably excellent antioxidant effect. In addition, the herbal composition complex fermented extract has an excellent effect of inhibiting the activity of elastase and collagenase and has an effect of improving wrinkles. Therefore, according to the present invention, the herbal extract, herbal medicine, herbal medicine, herbal medicine and herbal medicine combined fermented extract can be used as a functional raw material useful for antioxidation, anti-aging and anti-wrinkle effect.

1 is a graph showing the DPPH radical scavenging ability of the herbal composition complex fermentation extract according to the present invention.
2 is a graph showing the NO inhibitory activity of the herbal composition complex herbal extract according to the present invention.
FIG. 3 is a graph showing the elastase inhibitory activity of the herbal composition complex herbal extract according to the present invention.
FIG. 4 is a graph showing collagenase inhibitory activity of the herbal composition complex herbal extract according to the present invention. FIG.
FIG. 5 is a graph showing cytotoxicity of the herbal composition complex herbal extract according to the present invention. FIG.
FIG. 6 is a graph showing the moisturizing and holding power of a cosmetic composition containing the herbal composition complex herbal extract according to the present invention.

The present invention relates to an antioxidant or anti-aging cosmetic composition comprising an oriental herb complex, fermented herbal extract, killer herb extract, and herbal extract as an active ingredient.

The present invention also relates to a cosmetic composition for improving wrinkles comprising an oriental medicinal herb complex fermented extract as a active ingredient,

Hereinafter, the present invention will be described in detail.

The herbal composition of the present invention can be obtained by extracting it with a conventional method. Typical extraction methods include, but are not limited to, ultrasonic extraction, filtration, and reflux extraction.

In the present invention, it was obtained by the following method.

First, weeds, stools, kills and stones are washed with water to remove impurities, and then dried and pulverized at room temperature. The crushed greens, ground, kills and soils are extracted by immersion in a solvent. The extraction solvent is not limited thereto, but at least one solvent selected from water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof may be used, and water is preferable. The extracts were filtered, and then freeze-dried to obtain a final herbal medicine extract.

The herbal extract, herbal medicine, herbal medicine, and herbal medicine complex fermented extract of the present invention were obtained by inoculating a useful microorganism into the complex extract obtained as described above, followed by fermentation for 7 days. Useful microorganisms used for fermenting the complex extract include Lysinibacillus fusiformis nov., Sporolactobacillus nakayamae , Lactobacillus plantarum , Lactobacillus casei ), But are not limited thereto.

The herbal composition complex fermentation extract may be prepared by mixing the herbal extracts, carbs, kills, and gravel in a weight ratio of 0.5 to 3: 0.5 to 3: 0.5 to 3: 0.5 to 3, but is not limited thereto.

In one embodiment of the present invention, the various herbal extracts of herbal medicinal herbs combined with herbal medicinal herb extracts exhibited excellent DPPH radical scavenging activity and NO inhibitory activity, . In addition, it was confirmed that the herbal medicinal compound complex fermented extract exhibited a wrinkle-reducing effect by inhibiting the activity of elastase and collagenase. Therefore, according to the present invention, the fermented herbal extracts of Oriental herbaceous plants, herbaceous plants, killing plants and herbaceous plants can be used as functional raw materials useful for antioxidation, anti-aging, or wrinkle-reducing effects.

The cosmetic composition of the present invention may further contain additives commonly used in cosmetic compositions such as antioxidants, stabilizers, solubilizers, vitamins, pigments and antioxidants in addition to the active ingredients Herb, Crude, Cosmetic compositions can be prepared including conventional adjuvants and carriers such as perfumes. For example, the cosmetic composition may further contain an auxiliary component such as glycerin, butylene glycol, polyoxyethylene hardened castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin and the like .

The herbal composition, herbal composition, herbal composition, herbal composition and herbal composition complex herbal extract may be contained in an amount of 0.001 to 50% by weight, more preferably 0.01 to 30% by weight based on the total weight of the cosmetic composition, , The content can be freely selected depending on the application and purpose.

The cosmetic of the present invention may contain, in addition to the above-mentioned essential ingredients, other ingredients usually added to cosmetics, if necessary.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkylether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate , Alkyloyl taurine salts, N-acyl amino acid salts, POE alkyl ether carboxylates, alkylsulfosuccinates, sodium alkylsulfoacetates, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphate esters .

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Any of the above components may be blended within the range not to impair the objects and effects of the present invention, but is preferably 0.01-5% by weight based on the total weight of the composition, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, in addition to the above-mentioned extracts, the components conventionally used in cosmetic compositions, such as stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, suppositories, nutritional lotions, pastes, gels, creams, lotions, But are not limited to, a pack, a cleanser, a gel, a powder, a soap, a surfactant-containing cleansing, an oil, a powdered foundation, an emulsion foundation, a wax foundation and a spray. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritive cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray or a powder.

When the formulation is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracer, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component have.

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Propellants such as butane or dimethyl ether.

When the formulation is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation is a suspension, a liquid diluent such as water, ethanol or propylene glycol as the carrier component; Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Microcrystalline cellulose; Aluminum metahydroxide; Bentonite; Agar or tracant may be used.

When the formulation is an interface-active agent-containing cleansing, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

The present invention also provides an antioxidant or antioxidant food composition comprising a fermented extract of oriental herb complex, herbal medicine, herbal medicine, killer, and herbal composition as an active ingredient.

When the extract of the present invention is used as a food additive, it can be suitably used according to a conventional method such as adding the extract as it is, mixing it with another food or food ingredient, and the like.

The amount of the extract as the active ingredient may be suitably changed according to the purpose of use (prevention, health or therapeutic treatment), and the extract may be contained in an amount of 0.001 to 50% by weight based on the total weight of the food composition By weight, more preferably 0.1 to 30% by weight.

As a specific example, the extract of the present invention is added in an amount of not more than 30% by weight, preferably not more than 10% by weight based on the raw material. However, when it is intended for health and hygiene purposes or for the purpose of controlling health, it can be added in an amount below the above range, and there is no problem in terms of safety. Therefore, the active ingredient can be used in an amount exceeding the above range have.

There is no particular limitation on the kind of the food. Examples of the food to which the extract of the present invention can be added include meat products, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, Drinks, alcoholic beverages, vitamin complexes, and the like, all of which include health foods in a conventional sense.

When the food composition of the present invention is prepared as a beverage, it may contain additional ingredients such as various flavors or natural carbohydrates such as ordinary beverages. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharin and aspartame, and the like. The natural carbohydrate is contained in an amount of 0.01 to 10% by weight, preferably 0.01 to 0.1% by weight based on the total weight of the food composition of the present invention.

In addition to the above, the food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, , Carbonating agents used in carbonated beverages, and the like. In addition, the compositions of the present invention may include flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of the above additives is not limited to a great extent, but may be in the range of 0.01 to 0.1% by weight based on the total weight of the food composition of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example 1: Preparation of herbal extracts of Oriental herb, locust bean, and kangkok herb complex

1-1. Manufacture of herbal medicine complex extracts of Oriental herbs

(Pusan, Korea) were purchased and washed with water to remove impurities, followed by drying and pulverizing at room temperature. 200 g of ground crushed greens, crushed greens, kale and grains were mixed and then subjected to hot water extraction for 2 hours. The extract was filtered through a 0.45 μg filter and freeze-dried to obtain 12 g (4%) of the herbal medicine composite extract. The lyophilized extract was dissolved in tertiary distilled water before use, filtered through a 0.22 μg filter, and used in the following experiment.

1-2. Manufacture of herbal extracts of Oriental herb medicine

The Lysinibacillus fusiformis nov. MR-C2, Sporolactobacillus nakayamae MR-O2, which is a useful microorganism for fermenting the herbal medicine complex extract prepared in 1-1, O2), Lactobacillus plantarum MR-O3 and Lactobacillus casei MR-CD4 were separated and used. In order to ferment the herbal medicine composite extract, the strain was distributed from Myung-Rin Co., Ltd., and subcultured.

The herbal composition complex extract prepared in 1-1 above was diluted to a concentration of 10 mg / mL, and 1% sucrose was added thereto. Then, the useful microorganism, Lysinibacillus fusiformis MR-C2 nov. MR-C2), spokes with Lactobacillus Nakayama MR-O2 (Sporolactobacillus nakayamae MR- O2), Lactobacillus Planta room MR-O3 (Lactobacillus plantarum MR- O3), and Lactobacillus Kasei MR-CD4 (Lactobacillus casei MR -CD4) was inoculated and fermented for 7 days to produce the herbal medicine complex fermented extract of the present invention.

Example 2 Preparation of Cosmetic Products Containing Fermented Extract of Herb Medicinal Herb Extract

The ingredients shown in Table 1 below were mixed to prepare a cosmetic composition of the body lotion formulation containing the oriental herb, tea, kale and herbal combination herbal extracts prepared in Example 1 above.

[Table 1] Ingredients of body lotion containing herbal medicine complex fermented extract

Experimental Example 1. Antioxidative and antioxidative effects of the herbal composition complex fermented extract

Experimental Example 1-1. Identification of DPPH radical scavenging ability of herbal medicinal compound fermentation extract

In order to measure the antioxidant activity of the herbal composition complex fermented extract of the present invention, the DPPH radical scavenging ability of the herbal composition complex herbal extract prepared in Example 1 was evaluated.

Specifically, 100 μl of the herbal medicine complex fermentation extract and 1 ml of 5 × 10 ^-4 M DPPH solution were added to each well of the well microplate, and after stirring for 1 minute, the absorbance at 540 nm was measured using a microplate counter Respectively. The DPPH radical scavenging ability of the herbal medicine complex fermentation extract of the present invention was calculated based on the BHA's DPPH radical scavenging ability as 100% by using BHA as a positive control. The results are shown in Fig.

As shown in FIG. 1, the herbal composition complex fermented extract of the present invention showed a DPPH radical scavenging ability of 72,88% (0.1 mg / mL) as compared with that of the control, confirming that the antioxidant ability was excellent.

Experimental Example 1-2. Identification of NO inhibitory activity of herbal medicinal compound extracts

In order to measure the antioxidant activity of the herbal composition complex fermented extract of the present invention, the NO inhibitory activity of the herbal composition complex herbal extract prepared in Example 1 was evaluated.

Specifically, mouse RAW 264.7 cell line and melanoma cell line (B16F10) were cultured in DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ . LPS (lipopolysaccharide) was added to the culture solution of the mouse RAW 264.7 cell line and melanoma cell line (B16F10), and 100 μl of the cell culture solution and 100 μl of a grease reagent were mixed. After 10 minutes at room temperature After incubation, the NO concentration in the cell culture medium was measured by measuring the absorbance at 570 nm using a Vmax 96-well microplate spectrophotometer. The results are shown in FIG.

As shown in FIG. 2, the herbal medicine composite extract of the present invention has excellent NO inhibiting activity, and thus it can be effectively used as an anti-aging cosmetic composition.

Experimental Example 2. Confirmation of wrinkle improving effect of the herbal pharmacy complex fermentation extract

Experimental Example 2-1. Confirmation of Elastase Inhibitory Activity of Herb Extracts

Elastase is aged or excessively stimulated by external skin stimuli such as ultraviolet rays to excessively decompose elastin and weaken the collagen bonds, resulting in collagen and elastin network structure in the dermis tissue being broken, causing skin sagging and wrinkles The elastase inhibitory activity of the herbal medicine complex fermented extract of the present invention was measured in order to confirm the antioxidant effect of the herbal composition complex fermentation extract of the present invention.

Specifically, 500 μL of the herbal medicine complex fermented extract prepared in Example 1 was added to each test tube in a concentration-dependent manner, and porcine pancreas elastase, which was dissolved in 50 mM Tris-HCl buffer (pH 8.6) (L-Ala) 3-p-nitroanilide, 500 (500 μL / mL) was added to each test tube. mu g / mL) was added. After the reaction at 37 占 폚 for 20 minutes, the amount of p-nitroanilide produced from the substrate was measured (absorbance at 410 nm) to determine the inhibitory activity of the elastase of the herbal medicinal herb complex extract of the present invention Respectively. The inhibitory activity of elastase was expressed by the absorbance reduction rate of the addition of the sample solution and the non-addition of the sample solution. The results are shown in Fig.

As shown in FIG. 3, it was confirmed that the herbal medicinal herb complex fermented extract of the present invention had an excellent activity of inhibiting elastase.

Experimental Example 2-2. Confirming collagenase inhibitory activity of complex herbal extracts

Collagen is the main protein of all connective tissues such as skin, blood vessels, bones, teeth and muscles. Especially, reduction of collagen in skin causes aging. Since the collagenase is an enzyme that degrades collagen, the collagenase inhibitory activity of the herbal medicinal herb complex fermentation extract of the present invention was measured in order to confirm the antioxidant effect of the herbal composition complex fermentation extract of the present invention.

Specifically, the substrate, 4-phenylazo-benzyloxy-carbonyl-Pro-Leu-Gly-Pro-D-Arg (4-phenylazo-benzyloxy- / mL) was dissolved in 0.1 M tris-hydrochloric acid buffer (pH 7.5) to which calcium chloride (CaCl ₂ , 4 mM) was added, and 100 μl of the herbal composition complex fermentation extract prepared in Example 1 was added to each concentration. 150 μl of collagenase (200 μg / ml) was added thereto, and the mixture was allowed to stand at room temperature for 20 minutes. Then, 500 μl of 6% citric acid was added to stop the reaction. 1.5 ml of ethyl acetate was added thereto. Respectively. The inhibitory activity of collagenase was expressed by the absorbance reduction ratio of the sample solution and the non-additive solution. The results are shown in Fig.

As shown in FIG. 4, it was confirmed that the herbal medicine complex fermented extract of the present invention had an excellent activity of inhibiting collagenase.

Experimental Example 3. Cytotoxicity of the herbal extracts

In order to measure the safety of the herbal composition complex fermentation extract of the present invention, the cytotoxicity of the herbal composition complex herbal extract prepared in Example 1 was evaluated.

Specifically, mouse RAW 264.7 cell line and melanoma cell line (B16F10) were cultured in DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ . The mouse RAW 264.7 cell line and the melanoma cell line (B16F10) were collected and dispensed into a 96-well microplate, MTT assay was performed, and the absorbance was measured at 575 nm using a Vmax 96-well microplate spectrophotometer Cell viability was measured. The results are shown in Fig.

As shown in FIG. 5, the herbal medicine complex fermented extract of the present invention showed no cytotoxicity, and thus it was confirmed to be safe for use as a cosmetic composition.

Experimental Example 4. Confirmation of moisture retention ability of cosmetic composition containing herbal composition extract

In order to confirm the moisturizing and holding power of the cosmetic composition containing the herbal composition complex extract of the present invention, the skin cosmetic composition was applied to the skin and then the change in skin moisture content was measured.

First, 21 women who were between 20 and 55 years old were included in the study. Subjects were allowed to sit in a waiting room at room temperature and humidity conditions of 20 ~ 25 ℃ and humidity 40 ~ 60% for 30 minutes to adapt skin surface temperature and humidity to the environment of measuring space. Water intake during rest was limited. The subjects were allowed to rinse the entire test area, and then 2 μl / cm 2 of the cosmetic prepared in Example 2 was rinsed lightly on the test area, and then absorbed into the test area.

The skin moisture content of the subjects was measured immediately after the cosmetic composition was used, 4 hours later, 24 hours later, and 48 hours later. Skin moisture content was measured using a Corneometer CM825 (Couraage-Khhazaka electrinic GmbH, Germany). Measurements were taken three times through the sensor with a corneometry probe in contact with the skin and the mean value was used as the skin moisture content data. The results are shown in Fig.

As shown in FIG. 6, the water content of the cosmetic composition containing the herbal composition complex herbal extract of the present invention showed a statistically significant change at all points in comparison with the area where it was not used.

Experimental Example 5. Sensory Evaluation on Cosmetic Composition Containing a Herb Extract

After the experiment of Experimental Example 6, in order to confirm the effect of maintaining the moisturizing power of the cosmetic composition containing the herbal composition extract of the present invention, the sensory evaluation of the skin moisturizing, skin smoothness, The results are shown in Table 2 below.

At this time, 4 points were evaluated when the effect felt was excellent, and 0 points when the effect was not felt at all.

[Table 2] Sensory evaluation of cosmetic compositions containing herbal medicine complex extracts

As shown in Table 2, all of the cosmetic compositions containing the herbal composition complex extract of the present invention were evaluated to be higher than those of all the subjects in all the items, and skin hydration, skin smoothness and absorbency were particularly excellent.

Hereinafter, a cosmetic composition and a preparation example of a food composition containing the herbal composition complex fermented extract of the present invention as an active ingredient will be described, but the present invention is not limited thereto but is specifically described.

Formulation Example 1. Preparation of cosmetic preparation

Flexible longevity manufacturing

0.1% by weight of the herbal medicine complex fermented extract of Example 1, 5.2% by weight of 1,3-butylene glycol, 1.5% by weight of oleyl alcohol, 3.2% by weight of ethanol, 3.2% by weight of polysorbate 20, By weight, 1.0% by weight of a carboxyl vinyl polymer, 3.5% by weight of glycerin, a small amount of fragrance, a small amount of preservative and a remaining amount of purified water were mixed.

Milk lotion manufacturing

The composition of the present invention was prepared in the same manner as in Example 1 except that 0.1% by weight of the herbal composition complex fermented extract, 5.1% by weight of glycerin, 4.2% by weight of propylene glycol, 3.0% by weight of tocopheryl acetate, 4.6% by weight of liquid paraffin, A mixture of 2.5% by weight of oil, 1.6% by weight of polysorbate 60, 1.6% by weight of sorbitan sesquioleate, 0.6% by weight of propylparaben, 1.5% by weight of carboxyl vinyl polymer, a small amount of fragrance, a small amount of preservative, Milk lotion was prepared by a conventional method.

Nutrition cream manufacturing

0.5% by weight of the herbal composition complex fermented extract of Example 1, 4.0% by weight of glycerin, 3.5% by weight of petroleum jelly, 2.1% by weight of triethanolamine, 5.3% by weight of liquid paraffin, 3.0% by weight of squalane, 2.6% by weight of beeswax, , 3.2% by weight of polysorbate 60, 1.0% by weight of carboxyl vinyl polymer, 3.1% by weight of sorbitan sesquioleate, a small amount of fragrance, a small amount of preservative and remaining amount of purified water were mixed to prepare a nutritional cream by a conventional method .

Massage cream manufacturing

The composition of the herb complex complex fermented extract of Example 1 was prepared by adding 0.5% by weight of glycerin, 4.0% by weight of petroleum jelly, 3.5% by weight of petroleum jelly, 0.5% by weight of triethanolamine, 24.0% by weight of liquid paraffin, 3.0% by weight of squalane, 2.1% , 2.4% by weight of polysorbate 60, 1.0% by weight of a carboxyl vinyl polymer, 2.3% by weight of sorbitan sesquioleate, a small amount of fragrance, a small amount of preservative and a remaining amount of purified water were mixed to prepare a massage cream in a usual manner .

Formulation Example 2. Preparation of food preparation

Health food manufacturing

100 mg of the herbal composition complex fermented extract of Example 1, a proper amount of vitamin A, 70 g of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, 0.2 g of vitamin B12, 10 g of biotin, 1.7 mg of nicotinic acid amide, 50 g of folic acid, 0.5 mg of calcium pantothenate, 1.75 mg of ferrous sulfate, 0.82 mg of zinc oxide, 25.3 mg of magnesium carbonate, 15 mg of potassium phosphate monobasic 90 mg of potassium citrate, 100 mg of calcium carbonate, and 24.8 mg of magnesium chloride were mixed and granules were prepared and a health food was prepared according to a conventional method. At this time, although the composition ratio of the vitamin and mineral mixture is relatively mixed with the ingredient suitable for health food, it may be arbitrarily modified.

Health drink manufacturing

100 mg of vitamin C, 15 g of vitamin C, 100 g of vitamin E (powder), 19.75 g of iron lactate, 3.5 g of zinc oxide, 3.5 g of nicotinic acid amide, 0.2 g of vitamin A , 0.25 g of vitamin B1, 0.3 g of vitamin B2 and a predetermined amount of water were mixed and heated at 85 DEG C for about 1 hour with stirring. The solution was filtered to obtain a sterilized 2 L container, which was sterilized by sealing, A beverage was prepared. At this time, although the composition ratio of the ingredients suitable for the beverage is comparatively mixed, the mixture ratio may be arbitrarily varied according to the demand, the demanded country, the intended use, and the regional or national preference.

Claims (6)

Wherein the complex fermented extract is selected from the group consisting of Lysinibacillus fusiformis nov., Sporolactobacillus nakayamae , Lactobacillus nakayamae , Wherein the composition is fermented with at least one microorganism selected from the group consisting of Lactobacillus plantarum and Lactobacillus casei . The cosmetic composition for skin moisturizing according to claim 1, wherein the complex fermentation extract is extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. delete 2. The method according to claim 1, wherein the complex fermentation extract is extracted by mixing the herbal composition, the stomach, the kelp, and the gravel in a weight ratio of 0.5-3: 0.5-3: 0.5-3: 0.5-3. Cosmetic composition. Wherein the complex fermented extract is selected from the group consisting of Lysinibacillus fusiformis nov., Sporolactobacillus nakayamae , Lactobacillus nakayamae , Wherein the composition is fermented with at least one microorganism selected from the group consisting of Lactobacillus plantarum and Lactobacillus casei .
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