KR101595808B1 - 세포-표면 항원의 면역결합제를 동정하는 방법 - Google Patents
세포-표면 항원의 면역결합제를 동정하는 방법 Download PDFInfo
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- KR101595808B1 KR101595808B1 KR1020117014643A KR20117014643A KR101595808B1 KR 101595808 B1 KR101595808 B1 KR 101595808B1 KR 1020117014643 A KR1020117014643 A KR 1020117014643A KR 20117014643 A KR20117014643 A KR 20117014643A KR 101595808 B1 KR101595808 B1 KR 101595808B1
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| MX345395B (es) * | 2008-06-25 | 2017-01-30 | Esbatech Alcon Biomed Res Unit | Humanizacion de anticuerpos de conejos usando una estructura de anticuerpos universal. |
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| TWI386645B (zh) * | 2010-07-19 | 2013-02-21 | Univ Kaohsiung Medical | 可定量任何聚乙二醇分子與其修飾物之抗聚乙二醇表現細胞 |
| ES2723775T3 (es) | 2010-11-08 | 2019-09-02 | Ablynx Nv | Polipéptidos que se unen a CXCR2 |
| US8748587B2 (en) | 2011-06-02 | 2014-06-10 | Novartis Ag | Molecules and methods for modulating TMEM16A activities |
| CN103946236B (zh) * | 2011-11-23 | 2018-02-16 | 弗·哈夫曼-拉罗切有限公司 | 表达cd40l的哺乳动物细胞及其用途 |
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| CN105793705A (zh) * | 2013-09-30 | 2016-07-20 | X博迪公司 | 抗原受体的筛选测试 |
| CN111413298A (zh) * | 2020-05-27 | 2020-07-14 | 深圳市天安至远传感科技有限公司 | 一种光纤表面等离子体共振传感器、制备方法及检测系统 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US20030036092A1 (en) * | 1991-11-15 | 2003-02-20 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US5675063A (en) * | 1995-02-28 | 1997-10-07 | Loyola University Of Chicago | Immortalized rabbit hybridoma fusion partner |
| ATE390933T1 (de) | 1995-04-27 | 2008-04-15 | Amgen Fremont Inc | Aus immunisierten xenomäusen stammende menschliche antikörper gegen il-8 |
| CA2229043C (en) | 1995-08-18 | 2016-06-07 | Morphosys Gesellschaft Fur Proteinoptimierung Mbh | Protein/(poly)peptide libraries |
| EP0938506B1 (en) | 1996-07-16 | 2003-11-05 | Plückthun, Andreas, Prof. Dr. | Immunoglobulin superfamily domains and fragments with increased solubility |
| AU7171098A (en) * | 1997-05-01 | 1998-11-24 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US6060272A (en) * | 1997-05-07 | 2000-05-09 | Human Genome Sciences, Inc. | Human G-protein coupled receptors |
| FR2777007B1 (fr) * | 1998-04-01 | 2000-05-26 | Orstom | Mutants de glycoproteines d'enveloppes de retrovirus et leurs applications biologiques |
| US7157418B1 (en) * | 1998-07-22 | 2007-01-02 | Osprey Pharmaceuticals, Ltd. | Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders |
| US6905668B1 (en) | 1998-09-25 | 2005-06-14 | Tokyo Gas Company Limited | Diagnostic agents for pancreatic exocrine function |
| DE19852800C1 (de) * | 1998-11-16 | 2000-04-13 | Univ Albert Ludwigs Freiburg | Verfahren zur Herstellung von Antikörpern gegen ein Polypeptid, von dem die kodierende Nukleinsäure bekannt ist |
| AU768515B2 (en) * | 1999-01-13 | 2003-12-18 | Igeneon Krebs-Immuntherapie Forschungs- Und Entwicklungs-Ag | Use of antibodies for anticancer vaccination |
| US7417130B2 (en) | 2000-09-08 | 2008-08-26 | University Of Zurich | Collection of repeat proteins comprising repeat modules |
| ES2405944T3 (es) | 2000-11-30 | 2013-06-04 | Medarex, Inc. | Ácidos nucleicos que codifican las secuencias de inmunoglobulina humana reorganizadas a partir de ratones transcromoscómicos transgénicos zadas |
| CN105175535A (zh) | 2002-05-22 | 2015-12-23 | 艾斯巴技术-诺华有限责任公司 | 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法 |
| US20040067496A1 (en) * | 2002-10-07 | 2004-04-08 | Robert Pytela | System for production and screening of monoclonal antibodies |
| US20070122852A1 (en) | 2002-11-13 | 2007-05-31 | Micromet Ag | Method for identifying antigen specific b cells |
| JP4603894B2 (ja) * | 2002-12-03 | 2010-12-22 | ユセベ ファルマ ソシエテ アノニム | 抗体産生細胞を同定するためのアッセイ |
| DE10314412A1 (de) * | 2003-03-28 | 2004-10-14 | Genovac Ag | Genetische Immunisierung mit multiplen Expressionskonstrukten zur Herstellung von monoklonalen Antikörpern |
| CN1787798A (zh) | 2003-05-13 | 2006-06-14 | 日清奥利友集团株式会社 | 油性化妆品 |
| EP1623229A2 (en) * | 2003-05-15 | 2006-02-08 | Cytos Biotechnology AG | Selection of b cells with specificity of interest: method of preparation and use |
| CA2533830A1 (en) * | 2003-08-07 | 2005-02-24 | Epitomics, Inc. | Methods for humanizing rabbit monoclonal antibodies |
| JP2004107355A (ja) | 2003-10-20 | 2004-04-08 | Kokyu Alcohol Kogyo Co Ltd | 油性固形化粧料 |
| CN100451012C (zh) | 2003-11-20 | 2009-01-14 | 三菱瓦斯化学株式会社 | 液态环己烷-三羧酸酐 |
| CA2560074A1 (en) * | 2004-03-18 | 2005-11-03 | Board Of Regents, The University Of Texas System | Combinatorial protein library screening by periplasmic expression |
| AU2006220709B2 (en) * | 2005-03-04 | 2012-09-06 | Biogen Ma Inc. | Methods of humanizing immunoglobulin variable regions through rational modification of complementarity determining residues |
| CA2655511C (en) * | 2005-07-01 | 2017-03-21 | John Schrader | Methods of isolating cells and generating monoclonal antibodies |
| US20070134249A1 (en) * | 2005-12-08 | 2007-06-14 | Genitope Corporation | Combination therapy and antibody panels |
| US8017119B2 (en) | 2005-12-28 | 2011-09-13 | Daiichi Sankyo Company, Ltd. | Antibody against periostin, and a pharmaceutical composition comprising it for preventing or treating a disease in which periostin is involved |
| EP1918302A3 (en) | 2006-05-18 | 2009-11-18 | AvantGen, Inc. | Methods for the identification and the isolation of epitope specific antibodies |
| NZ573557A (en) * | 2006-06-02 | 2010-08-27 | Regeneron Pharma | High affinity antibodies to human il-6 receptor |
| ES2820837T3 (es) | 2006-07-10 | 2021-04-22 | Esbatech Alcon Biomed Res Unit | Anticuerpos scFv que pasan las capas epitelial y/o endotelial |
| CN101675074B (zh) * | 2006-12-20 | 2016-02-24 | Mmr全球公司 | 抗体及其制备和使用方法 |
| JP2010222255A (ja) * | 2007-06-18 | 2010-10-07 | Mitsubishi Tanabe Pharma Corp | 抗体のスクリーニング方法およびそれにより得られる抗体 |
| JP5022818B2 (ja) | 2007-08-10 | 2012-09-12 | 株式会社 資生堂 | 毛髪化粧料 |
| MX345395B (es) | 2008-06-25 | 2017-01-30 | Esbatech Alcon Biomed Res Unit | Humanizacion de anticuerpos de conejos usando una estructura de anticuerpos universal. |
| KR101737466B1 (ko) | 2008-06-25 | 2017-05-18 | 에스바테크 - 어 노바티스 컴파니 엘엘씨 | Ｖｅｇｆ를 억제하는 안정하고 가용성인 항체 |
| WO2009155723A2 (en) | 2008-06-25 | 2009-12-30 | Esbatech, An Alcon Biomedical Research Unit Llc | STABLE AND SOLUBLE ANTIBODIES INHIBITING TNFα |
| CA2744103C (en) | 2009-02-24 | 2018-01-02 | Esbatech, An Alcon Biomedical Research Unit Llc | Methods for identifying immunobinders of cell-surface antigens |
| JP5926896B2 (ja) | 2010-09-17 | 2016-05-25 | 株式会社 資生堂 | 唇用固形化粧料 |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004271336A (ja) * | 2003-03-07 | 2004-09-30 | Hiroo Iwata | 細胞に発現する表面抗原を迅速に同定するための分析方法 |
Non-Patent Citations (2)
| Title |
|---|
| egal, D. M. et al., ‘The Measurement of Specific Cell:Cell Interactions by Dual-Parameter Flow Cytometry’, Cytometry, (1984), Vol. 5, pp 169-181.* |
| Smith, D. D. et al., ‘Optimization of Cellular ELISA for Assay of Surface Antigens on Human Synoviocytes’, Bio Techniques, (1997), 22, pp 952-957* |
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| US9221905B2 (en) | 2015-12-29 |
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| KR20110136783A (ko) | 2011-12-21 |
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