KR101485245B1 - Liquid phase nutritional supplements for apple cultivation and process for preparing the same - Google Patents

Abstract

The present invention relates to a liquid nutrient for apple cultivation containing an apple and a starfish component and a method for preparing the same. More specifically, the present invention relates to a liquid nutrient for apple cultivation which can contain nutrition ingredients containing apple and starfish, And a manufacturing method thereof.
The nutrient according to the present invention can prevent the growth promotion and physiological disorder of apple trees by supplying proper nutrients to apple trees, and can produce apple of excellent quality. Also, since the nutrient according to the present invention is manufactured by using papasia, apples and starfish with low value of commodities as main materials, the cost and environment can be expected to be improved by recycling the resources that are discarded or discarded.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid nutrition supplements for apple cultivation,

The present invention relates to a liquid nutrient for apple cultivation containing an apple and a starfish component and a method for preparing the same. More specifically, the present invention relates to a liquid nutrient for apple cultivation which can contain nutrition ingredients containing apple and starfish, And a manufacturing method thereof.

Apples belong to the family Rosaceae and are 25 kinds of apple trees. The annual production of the apples is 32 million tons per year. The varieties are divided into early, early and late-life varieties according to harvesting time. It is an alkaline food rich in minerals such as vitamin C, potassium, and sodium with fewer proteins and fats. It is used as raw food for processed foods such as jam, juice, cider, and alcohol.

In the cultivation of such apples, attempts have been made to increase the cultivation efficiency or to produce apples of higher quality. For example, Korean Patent No. 10-1014330 discloses a method of using a fertilizer or a nutrient using a mineral component Korean Patent No. 10-0565901 discloses a method of cultivating an apple rich in calcium by using an ionized calcium preparation prepared from a shell as a raw material. In Korean Patent No. 10-1235745, And a method of growing vanadium-containing functional apple by applying colloidal vanadium. The methods of the prior art are a method of providing nutrients necessary for the production of apples through fertilizer, which can increase the quality of apples or produce apples containing functional ingredients.

The inventors of the present invention have developed a method for increasing the quality of apples, which is capable of cultivating apples more effectively in addition to the above-mentioned prior arts, and at the same time, contributing to solving environmental problems by using materials that are abandoned or have poor utilization And to develop a method for expecting cost reduction.

In fruit-growing farms where apples are cultivated, there are cases where it is difficult to treat them because the amount of pods that are opened after the pods and apples that are low in commerciality are generated in large quantities. However, these apples contain almost all the nutritional ingredients, so it would be very effective if applied to plant nutrients. We expected that apple trees would have a good effect because they have all the ingredients needed to produce apples.

And starfish, which is an outlaw of the sea and troublesome to fishermen, also contains various nutrients, especially large amounts of calcium and amino acids, as living organisms.

As a result of efforts to develop a nutrient that can effectively grow apples using abandoned apples and starfish, it has been found that the extracts and fermentation processes under specific conditions can be used for the growth of apple trees from apple and starfish, The present invention has been accomplished by confirming that it is possible to produce high quality apple having excellent content of calcium, vitamin, sugar content and the like as well as promoting the growth when the apple is cultivated by using it.

Accordingly, it is an object of the present invention to provide a nutrient for apple cultivation which can produce apples of excellent quality, and in particular, to provide a nutrient for cultivation of apples that can utilize an organism having low value in industry, which is in a state of being thrown away or discarded.

In order to accomplish the above object, the present invention provides a method for preparing a starch, comprising mixing 500 parts by weight of apple extract, 150-250 parts by weight of starfish powder and 5-20 parts by weight of glacial acetic acid; Stirring the mixture of apple extract, starch powder and glacial acetic acid at 40 to 60 DEG C for 12 hours to 3 days; Mixing the yeast culture solution with a mixture of apple extract, starfish powder and glacial acetic acid, and mixing 3 to 10 parts by weight of yeast culture solution based on 500 parts by weight of the apple extract; And fermenting the mixture of the apple extract, starfish powder, glacial acetic acid and yeast culture with stirring at 40 to 60 캜 for 1 to 5 days, thereby producing a liquid nutritional product for apple cultivation.

In the present invention, the apple extract is prepared by mixing 100 parts by weight of apple, 5 to 10 parts by weight of licorice and 0.5 to 5 parts by weight of sulfur, extracting at 200 to 300 DEG C for 12 hours to 2 days, and filtering.

The present invention also provides a liquid nutrient for apple cultivation produced according to the above-described method.

The nutrient according to the present invention can prevent the growth promotion and physiological disorder of apple trees by supplying proper nutrients to apple trees, and can produce apple of excellent quality.

In addition, since the nutrient according to the present invention is produced by using as a main ingredient a poultry, an apple with a low value of a commodity, and a starfish, the cost and environment can be expected to be improved by recycling waste materials.

1 is a process diagram showing a process for producing a liquid nutrient according to the present invention.
FIG. 2 is a photograph taken by inoculating and culturing a liquid nutrient according to the present invention on a solid medium. The growth of fermenting microorganisms such as yeast can be confirmed.
FIG. 3 is a graph comparing the leaf length, leaf width and leaf thickness of apples with respect to apples grown using the liquid nutrient according to the present invention and untreated control. FIG.
FIG. 4 is a graph showing the comparison of apical length, exotherm, and diameter of apples in the apple cultivation and non-cultivation control using the liquid nutrient according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is characterized in that a liquid nutrient for apple cultivation is produced by using an apple and a starfish. Liquid nutrients are produced in a liquid form containing a lot of water, unlike compost type nutrients that are near solid form.

FIG. 1 is a process diagram showing a process for producing a liquid nutrient according to the present invention. First, an apple extract is prepared.

In the present invention, apples are used for the cultivation of apples. Apples, of course, contain essential nutrients necessary for the elimination of apples. Therefore, turning this nutrient into an easily absorbable form of apple tree will be the best nutrient source. For this purpose, apples are prepared in the form of extracts and used to make nutrients.

In view of the economical aspect and recycling of the discarded material, it is preferable to use an apple which has been broken down after the tearing and which is broken and has a low commerciality. The same effect can be expected when using ordinary apple sold on the market.

In the present invention, the apple extract is preferably prepared by mixing 100 parts by weight of apple, 5 to 10 parts by weight of licorice and 0.5 to 5 parts by weight of sulfur, heating extraction at 200 to 300 DEG C for 12 hours to 2 days, and filtering.

Since the apple contains a large amount of water, the useful ingredient can be extracted by heating without adding water separately. The addition of licorice to enhance the sugar content of the apple extract is desirable for proper growth and activity of microorganisms such as yeast during the fermentation process. Sulfur is added for sterilization, detoxification, growth promotion and so on. The extraction temperature and the extraction time are preferably in the above range in consideration of extraction efficiency and physical properties of the extract.

Next, apple extract, starfish powder and glacial acetic acid are mixed. Specifically, 500 parts by weight of apple extract, 150-250 parts by weight of starfish powder and 5-20 parts by weight of glacial acetic acid are mixed.

Starfish contains a large amount of calcium and amino acids needed for apple growth. In the present invention, the calcium phosphate is extracted from such a starfish, and the protein is converted into a form that is easily absorbed by the amino acid through amino acid. For this purpose, it is preferable to use the starfish in powder form. If the dried starfish is pulverized into 50 to 200 mesh, the starfish powder suitable for the present invention can be produced.

In the present invention, glacial acetic acid enables effective dissolution of calcium from starfish. It is preferable that the true amount of glacial acetic acid is 5 to 20 parts by weight based on 500 parts by weight of the apple extract. If the added amount of glacial acetic acid is too low, the calcium elution efficiency is lowered, and if it is too much, the pH of the mixture becomes too low, which may cause problems in the yeast fermentation process.

Thereafter, the nutrient is produced through two steps. The first step is the step of extracting and aging calcium by stirring the apple extract, starfish powder, and glacial acetic acid, and the second step is fermentation by adding the yeast culture solution thereto.

The temperature at the first stage of stirring is preferably 40 to 60 ° C, more preferably 48 to 52 ° C. The stirring time is preferably 12 hours to 3 days, and it is most appropriate to carry out the stirring for about one day.

In the second step, yeast culture solution is mixed into a mixture of starfish powder, apple extract, and glacial acetic acid. In consideration of the fermentation efficiency and the fermentation broth, it is preferable to mix 3 to 10 parts by weight of yeast culture solution based on 500 parts by weight of the apple extract .

In the present invention, yeast culture broth can be prepared by inoculating and culturing yeast in a liquid medium. As the yeast, Saccharomyces cerevisiae and the like can be used. In addition to the medium such as YPD and YM, the medium for the yeast growth can be used as the medium. For culturing conditions, it is preferable to incubate at 28 to 32 DEG C for 1 to 3 days.

In the second stage, when fermenting a mixture of starfish powder, apple extract, glacial acetic acid and yeast culture, the stirring temperature is preferably 40 to 60 ° C, and the time is preferably 1 to 5 days. More preferably, fermentation is carried out while stirring at 48 to 52 ° C for 2 to 4 days, and it is most appropriate to carry out fermentation at 50 ° C for 3 days. In this process, the fermentation by yeast is carried out, and protein of starfish is amino acidified, and the apple tree is converted into a form that is easy to absorb.

The nutrient produced through the second step can be used immediately, and in some cases, a postnatal step may be added. This step is a step to induce the growth and further fermentation of microorganisms such as yeast. For example, some bran (residue left after removing wheat from flour) and sugar (such as soil sugar) are dissolved in water and mixed well in the fermentation mixture Method can be used. In this case, it is preferable to use 100 g to 1 kg of ground sugar per 500 L of the fermented mixture.

The liquid nutrient of the present invention is preferably foliar application about 1 to 6 times between May and September. For example, in May, July, August, and September, you can foliage once a month. The liquid nutrient of the present invention is preferably diluted to 1/100 to 1 / 2,000 by using water, and the amount of the nutrient to be used is preferably 100 to 2,000 liters per 500 square meters based on 1/1000 dilution.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

[Example]

1. Preparation of apple extract

In the present embodiment, papusha was opened and opened and low quality apples which were difficult to commercialize or sell were mainly used.

An apple extract was prepared by adding 1,000 ㎏ of apple residue or phage, about 80 ㎏ of licorice, and about 20 ㎏ of sulfur to an extraction tank, extracting it at 250 캜 for 24 hours, and then filtering it.

2. Manufacture of liquid nutrients

200 kg of dried starfish powder (50 to 100 mesh) and 10 kg of glacial acetic acid were mixed with 500 kg of the apple extract prepared above, and the mixture was kept at 50 캜 with stirring for 24 hours.

A mixture of starch powder, apple extract, glacial acetic acid and yeast culture was prepared by mixing 5 kg of yeast culture solution based on 500 kg of apple extract and mixing starch powder, apple extract and glacial acetic acid, Followed by stirring at 50 ° C for 72 hours for fermentation.

[Test Example]

1. Nutrient application

The apples were cultivated in the apple cultivation area of about 15,000 pyeong located in Jangsu-gun, Jeollabuk-do. At this time, the apple variety was Hongro or Fuji.

In May, July, August, and September, the liquid nutrient of the above example was diluted to 1 / 1,000 (using water) and foliar application was made.

2. Nutritional Analysis

The composition analysis of the liquid nutritive agent prepared in the above Example was commissioned to the First Analysis Center, and the results are shown in Table 1.

ingredient content Isoleucine 2,342 (mg / kg) Leucine 3,540 (mg / kg) Lysine 3,797 (mg / kg) Methionine 1,812 (mg / kg) Cysteine 1,704 (mg / kg) Phenylalanine 2,095 (mg / kg) Tyrosine 1,912 (mg / kg) Threonine 2,612 (mg / kg) Tryptophane 1,082 (mg / kg) Valine 2,101 (mg / kg) Histidine 962.5 (mg / kg) Arginine 3,835 (mg / kg) Glutamic acid 5,970 (mg / kg) Glysine 2,722 (mg / kg) Proline 2,935 (mg / kg) Serine 1,932 (mg / kg) Taurine - Crude protein 4.64 (%) Calcium (Calsium) 7.8 (%) Magnesium 0.92 (%) Manganese 0.91 (mg / kg) Zinc 1.73 (mg / kg) Phosphorous 275.6 (mg / kg)

In addition, when the nutrient of the above example was inoculated into the solid medium and cultured, the growth of the microorganism expected to be yeast could be confirmed as shown in FIG.

3. Analysis of apple cultivation effect

The apple leaves and apples in the case of cultivation (control) and the control (control) as described above were compared and compared.

As a result, as shown in Tables 2 and 3, and FIGS. 3 and 4, it was confirmed that the growth of apple leaves was increased due to fertilizer fertilization, and the quality (size, color, vitamin content, etc.) of apples was improved. In particular, the content of vitamins in apples was considerably increased (see Table 4), indicating that the color and sugar content of the fruits were excellent.

Fuji apple Control Test section Leaf 82.98 mm 87.83 mm (6% increase) Leaf width 53.05 mm 55.00 mm (4% increase) Leaf thickness 0.36 mm 0.38 mm (8% increase)

Fuji apple Control Test section Cuff length 24.63 mm 23.28 mm (5% reduction) Exaggeration 72.12 mm 79.32 mm (10% increase) Hyperbolic 82.51 mm 87.78 mm (6% increase)

Item Control Test section Calories (cal) 55.9 56.3 moisture(%) 82.2 81.4 protein(%) 0.19 0.1 Lipid (%) 0.07 0.05 Carbohydrate (%) 15.74 15.9 Dietary Fiber(%) 0.59 0.54 Sodium (mg) 4.2 8.7 Calcium (mg) 2.6 3.54 Potassium (mg) 100.1 248.3 Phosphorus (mg) 8.6 7.4 Iron (mg) 0.27 0.46 Magnesium (mg) 1.52 2.07 Vitamin A (IU) 2.43 41.1 (17 times stronger) Vitamin B1 (mg) 0.03 0.13 (4.2 times strengthening) Vitamin B6 (mg) 0.05 0.17 (3.4 times stronger) Vitamin C (mg) 6.8 12.8 (strengthened 1.9 times) Vitamin E (mg) 0 0

Claims (3)

500 parts by weight of apple extract, 150-250 parts by weight of starfish powder and 5-20 parts by weight of glacial acetic acid;
Stirring the mixture of apple extract, starch powder and glacial acetic acid at 40 to 60 DEG C for 12 hours to 3 days;
Mixing the yeast culture solution with a mixture of apple extract, starfish powder and glacial acetic acid, and mixing 3 to 10 parts by weight of yeast culture solution based on 500 parts by weight of the apple extract;
Fermenting a mixture of apple extract, starfish powder, glacial acetic acid and yeast culture with stirring at 40 to 60 DEG C for 1 to 5 days; And
Bran and sugar in water to prepare a fermentation mixture, and then growing the mixture.
The method according to claim 1,
Wherein the apple extract is prepared by mixing 100 parts by weight of apple, 5 to 10 parts by weight of licorice and 0.5 to 5 parts by weight of sulfur, and then extracting the mixture at 200 to 300 DEG C for 12 hours to 2 days and filtering the mixture. ≪ / RTI >
A liquid nutritional product for apple cultivation produced according to the method of claim 1 or 2.

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