KR101458274B1 - Cosmetic Composition Comprising Fermented Ginseng Extracts - Google Patents

Abstract

The present invention relates to a composition for preventing skin aging and improving skin moisturization, and more particularly, to a composition for inhibiting skin aging and improving skin moisturization, and more particularly, a ginseng fermented extract containing lipid components such as phytosphingosine and ceramide, obtained by fermenting ginseng as a Pichia pastifer, Has an antioxidative activity due to its antioxidative activity, and has an effect of preventing skin aging. The skin fibroblast activity improves skin moisture retention and reduces moisture evaporation. Therefore, the ginseng fermented extract of the present invention is a composition for preventing skin aging and improving moistur As shown in FIG.

Description

[0001] Cosmetic Composition Comprising Fermented Ginseng Extracts [

The present invention relates to a cosmetic composition for preventing skin aging and improving skin moisturization comprising an extract of fermented ginseng as an active ingredient.

In the case of skin aging, it is divided into two types, intrinsic aging and photoaging (EBDoris, Cosmetics & Toiletries., 111, pp31-37, 1996) Although it is difficult to artificially control it due to the influence of element, it is natural to appear with age. However, photoaging is a phenomenon caused by external environmental factors, and it can be said that artificial control is relatively easy. Exogenous aging is characterized by the formation of excess reactive oxygen species by ultraviolet rays in the skin exposed to ultraviolet rays by the sunlight and the reduction of antioxidant such as vitamin E, vitamin E, C, glutathione and ubiquinol in the body, It is an unbalanced story. As a result, lipid peroxidation, protein oxidation, proteolytic enzyme activation, chain cleavage and abnormal cross-linking of collagen and elastin, hyaluronic acid chain cleavage, promotion of melanin production, DNA oxidation, (Oikarinen, A., Photodermatol, Photoimmunol. Photomed., 7 (1), pp3-4, 1990; Yamauchi, M., J. Invest. Dermatol., 97, pp 938-941, 1991).

Ginseng (Panax Ginseng) is a herbal medicine as well as in the oriental medicine and Acanthopanax of ginseng have been widely used as remedies (Araliaceae) (Panax), is classified into fresh ginseng, white ginseng and red ginseng, depending on its state. Fresh ginseng is the root of ginseng that is not dried after harvest. White ginseng is the roots of dried ginseng after removing the root roots of fresh ginseng and peeling it off. Red ginseng is produced by steam-drying ginseng roots. Saponin of ginseng promotes the secretion of catecholamine in the adrenal gland and inhibits the lipid peroxidation process. It has antioxidant activity, and has a protective effect against radiation, a chromosome abnormality and micro-nuclear defense effect by alkylating agent, a mutation reduction in cultured cells, And the effect of reducing cell deformation has been reported.

However, since ginseng and red ginseng have little effect on skin even when extracting active ingredients through modern extraction techniques such as hydrothermal, supercritical, or ultrasonic waves, various bio-conversion technology There is a need to develop a technique capable of effectively extracting the information.

It is an object of the present invention to provide a cosmetic composition containing a ginseng fermented extract for skin skin fibroblast activity, skin moisture retention, skin aging prevention and skin moisturizing improvement effect through an effect of suppressing moisture evaporation power.

In order to achieve the above object, the present invention when blood ginseng extract teeth Perry (Pichia The present invention provides a cosmetic composition for preventing skin aging and improving skin moisturization comprising an extract of fermented ginseng produced by fermenting with a ciferrii strain as an active ingredient.

The type of the ginseng used in the present invention, ginseng (Panax Ginseng , Panax quinquefolium , Panax notoginseng and Panax Japonicus ). Among them, red ginseng is preferable, but the present invention is not limited thereto.

The ginseng extract is prepared by mixing 5 to 20 parts by volume of water with respect to the dry weight of ginseng, anhydrous or lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform or 1,3- , An organic solvent such as glycerol, propylene glycol, methyl acetate, nucleic acid, benzene, and petroleum ether as an extraction solvent, and heating the mixture at 50 to 100 ° C for 1 to 5 hours in an extractor equipped with a reflux condenser. The ginseng extract is preferably an ethanol-soluble extract obtained by using ethanol as an extraction solvent, but is not limited thereto.

Then, after filtration with a filter cloth, the residue is further extracted one or more times by the same method, and the extracts are combined, concentrated under reduced pressure, and then lyophilized or spray-dried to obtain dry extract of ginseng.

The ginseng extract may be subjected to a sterilization process for smooth fermentation by yeast. The ginseng extract may be added to yeast cells and cultured to isolate the extract to obtain a ginseng fermented extract. The yeast used in the fermentation of the present invention may be selected from the group consisting of Pichia sp. Or more and may be used for fermentation. In particular, Pichia cepi is preferable.

Preferably, the ginseng extract is added to the culture medium for the culture of Pichia csperii in an amount of 1 to 20 wt% based on the total weight of the culture medium, but is not limited thereto.

In a preferred embodiment of the present invention, in order to ferment the ginseng extract, P. tehnsperi is inoculated (about 10 ⁵ to 10 ⁷ cells / ml) to a basic medium (for example, YM medium) containing ginseng extract It is preferable to incubate for 30 to 120 hours while maintaining the pH of 5.5 to 6.5, the temperature of 25 to 35 ° C, and the aeration amount of 0.05 to 0.5 VVM, but the present invention is not limited thereto. Then, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast cells, followed by concentration under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization to obtain a ginseng fermentation extract.

The ginseng fermentation extract obtained as described above is preferably blended in an amount of 0.0001 to 99.0% by weight based on the total cosmetic composition, but is not limited thereto. If the content of the ginseng fermentation extract is less than 0.0001% by weight, the effect on skin aging and moisturizing is not exhibited. If it exceeds 99.0% by weight, the synergistic effect of the effect is inferior to the application amount, .

The cosmetic composition to which the cosmetic composition of the present invention can be applied is not particularly limited, and any conventional formulation can be applied. For example, the composition may be formulated into ointments for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. Herein, the ointment for external use is prepared so as to contain 50.0 to 97.0% by weight of petrolatum and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate in addition to the ginseng fermentation extract of the present invention, and the softening water is prepared by adding propylene glycol, glycerin, 1.0 to 10.0% by weight of polyhydric alcohols, and 0.05 to 2.0% by weight of a surfactant such as polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, and the like. The nutritional lotion and nutritional cream may contain, in addition to the ginseng fermentation extract, 5.0 to 20.0% by weight of oils such as squalane, vaseline and octyldodecanol, and 3.0 to 15.0% by weight of wax components such as cetanol, stearyl alcohol and beeswax, May contain 5.0 to 30.0% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like. The massage cream may further contain 30.0 to 70.0% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like, and the pack may comprise a peel off pack containing 5.0 to 20.0% by weight of polyvinyl alcohol Or a wash-off pack containing 5.0 to 30.0% by weight of pigment such as kaolin, talc, zinc oxide, titanium dioxide or the like in a general emulsified cosmetic.

Meanwhile, the skin aging prevention and moisturizing composition containing the fermented ginseng extract of the present invention may contain conventional ingredients to be formulated in ordinary skin formulations such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, Coloring matters, preservatives, fragrances and the like can be applied and compounded as necessary.

The present inventors prepared ginseng fermented extract by removing ginseng fermented by extracting ginseng extract with ethanol as described above and then fermenting the extract with P. fischeri. The anti-aging and skin moisturizing effects of the ginseng fermentation extract were investigated. As a result, the ginseng fermentation extract, which can contain lipid components such as phytosphingosine and ceramide by fermenting ginseng with P. fischeri ferment, (See Table 1), and it was found that the skin fibroblast activity improves the skin moisture retention and the moisture evaporation reduction is excellent (see Table 2 and Table 3). Therefore, the ginseng fermented extract of the present invention can be effectively used as an effective ingredient of a cosmetic composition for preventing skin aging and improving skin moisturization.

In addition, the present invention provides a pharmaceutical composition for preventing skin aging and improving skin moisturization, which contains, as an active ingredient, ginseng fermented extract prepared by fermenting ginseng extract with a Pichia pasta strain.

When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition may further comprise a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one usually used at the time of formulation, and includes lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995). The pharmaceutical composition may be administered orally or parenterally, preferably parenterally, more preferably topical application by application.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on such factors as formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition is in the range of 0.001 to 100 mg / kg on an adult basis. When the composition is an external preparation, it is preferably applied once to five times a day in an amount of 1.0 to 3.0 ml on an adult basis and continued for at least one month. However, the dosage is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be formulated into a unit dosage form by using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in oils or aqueous media, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

The present invention also provides a composition for health food for preventing skin aging and improving skin moisturization, which contains ginseng fermented extract prepared by fermenting ginseng extract with a P. articherii strain as an active ingredient.

When the composition of the present invention is prepared as a composition for a health food, it may contain components that are conventionally added in food production, and may include, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents . Examples of the above-mentioned carbohydrates include conventional saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose, sucrose and oligosaccharides, and polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol, erythritol And the like. Natural flavoring agents such as tau martin, stevia extract, and synthetic flavoring agents may be used as flavoring agents. For example, when the health food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, natural extract, etc. in addition to the ginseng fermentation extract of the present invention.

The ginseng fermented extract of the present invention has an excellent antioxidative and cell proliferative activity and has an excellent moisturizing effect by improving moisture retention, and thus is useful as an active ingredient of a pharmaceutical or health food composition for preventing skin aging and improving skin moisturization Can be used.

The ginseng fermented extract according to the present invention promotes the activity of fibroblasts of the skin to improve the retention of water in the skin, has no cytotoxicity, and has an excellent effect of improving skin moisturizing, and thus can be effectively used for skin aging and moisturizing.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1> Pichia  Preparation of red ginseng extract fermented with yeast

After drying 1 kg of 6-year old red ginseng, it was washed three times with 2.5 ℓ of ethanol at room temperature for 2 days, and then the residue was removed by filtration. Then, it was concentrated under reduced pressure to obtain 120 g of red ginseng extract. In addition, Pichia ciferrii were cultured in YM optimal medium (red ginseng extract 100 g / l, KNO ₃ 3 g / l, (NH ₄ ) ₂ SO ₄ 0.5 g / l, MgSO ₄揃 7H ₂ O 0.3 g / , CSL 3 g / l, LS-300 1 g / l) and incubated at 25 ° C for 4 days at 250 rpm. After incubation at 4 ° C for 2 days, the cells were recovered by centrifugation. Then, after acylation, the extract was extracted with Caprylic / Capric Triglyceride at 60 ° C for 3 hours and filtered to obtain a Pichia pasty fermented red ginseng extract.

< Comparative Example  1> Preparation of red ginseng extract

After drying 1 kg of 6-year old red ginseng, it was washed three times with 2.5 ℓ of ethanol at room temperature for 2 days, and then the residue was removed by filtration. Then, it was concentrated under reduced pressure to obtain 120 g of red ginseng extract.

< Comparative Example  2> Pichia  Production of genus yeast extract

Blood teeth when ferry strain YGM optimal medium (glycerol, _{100 g / ℓ, KNO 3 3} g / ℓ, (NH 4) 2 SO 4 0.5 g / ℓ, MgSO 4 · 7H 2 O 0.3 g / ℓ, NaCl 0.5 g / l, CSL 3 g / l, LS-300 1 g / l) and incubated at 25 ° C for 4 days at 250 rpm. After incubation at 4 ° C for 2 days, the cells were recovered by centrifugation. Then, after acylation, the extract was extracted with caprylic / capric triglyceride at 60 ° C for 3 hours and filtered to obtain a Pichia pasta fermented extract.

< Experimental Example  1> Pichia Seperry  Identification of Antioxidant Activity of Fermented Red Ginseng Extract

In order to examine the antioxidative effects of the extracts prepared in Example 1 and Comparative Examples 1 and 2, a free radical scavenging activity test was performed. The free radical scavenging activity test was carried out by DPPH (1, 1-dipheny1-2-picrylhydrazy1, Sigma) (Blois, MS Nature 181, 1190, 1958). DPPH shows a maximum absorption at 517 nm in the presence of radicals as a relatively stable free radical, and lost its absorption when eliminated. DPPH was dissolved in methyl alcohol at a concentration of 0.15 mM. First, the extracts prepared in Example 1 and Comparative Examples 1 and 2 were prepared at each concentration (0.1, 1, 10, 100, 1000, 10,000 / / ml) and 2 ml each was added thereto. For 10 minutes, and the absorbance was measured at 517 nm. At this time, purified water was used as a control group. Thereafter, the free radical scavenging rate was calculated using the absorbance in accordance with the following equation (1), and it was calculated by repeating the test five times.

[Equation 1]

Free radical scavenging rate (%) = {100- (absorbance of blank in test group / absorbance in control group)} 100

As a result, it was found that red ginseng extract fermented with Pichia csperi isolate had the most excellent free radical scavenging ability and thus had excellent anti-aging effect on skin (Table 1).

extract Concentration (/ / ml) Free radical scavenging rate (%) Example 1 0.1 5.7 One 10.4 10 37.5 100 54.4 1,000 66.9 10,000 80.4 Comparative Example 1 0.1 0.7 One 1.2 10 10.1 100 15.5 1,000 20.1 10,000 22.4 Comparative Example 2 0.1 2.7 One 3.3 10 22.1 100 27.5 1,000 38.1 10,000 48.7

< Experimental Example  2> Pichia Seperry  Identification of cell proliferation activity of fermented red ginseng extract

To measure the cell proliferation activity and cytotoxicity of the extract prepared in Example 1 and Comparative Examples 1 and 2, human fibroblasts were cultured and MTT reduction method (3, 4,5-dimethylthiazol-2-yl ) -2,5-diphenyltetrazolium bromide reduction method), and the formazan product was extracted with isopropyl alcohol and the absorbance at 570 mn was measured and compared. As a positive control, a medium containing 10% fetal bovine serum (FBS) was used. As a negative control, FBS-free medium was used. As an experimental control group, a culture without an extract was used Respectively. The viability of the cells was calculated numerically using the following equation (2), and the results were calculated by repeating the experiment five times.

&Quot; (2) &quot;

Cell proliferative capacity (%) = absorbance in each sample group / absorbance in the control group

As a result, it was confirmed that the extract of red ginseng fermented as Pichia peperii strain showed the most excellent cell proliferation ability and thus it was excellent in anti-aging effect, and was also a safe substance without cytotoxicity (Table 2).

extract Concentration (/ / ml) Cell proliferation activity (%) Example 1 0.1 111.9 One 120.6 10 123.6 100 138.4 1,000 152.1 Comparative Example 1 0.1 97.2 One 96.6 10 98.6 100 102.4 1,000 103.1 Comparative Example 2 0.1 99.9 One 100.6 10 102.6 100 115.4 1,000 112.1

< Experimental Example  3> Pichia Seperry  Of fermented red ginseng extract Moisture holding ability  Confirm

In order to evaluate the skin moisturizing power of the essence prepared in Example 2, the essence described in Table 1 was applied to the skin in a predetermined amount, and then the moisture content of the stratum corneum was measured and evaluated. As a measuring device, a skin moisture content measuring device (skin surface hygrometer, model name: SKICON-200, Japan IBS) was used. Specifically, in a constant temperature and humidity chamber having a temperature of 25 ° C and a relative humidity of 45%, the essence of Example 2 was applied to a predetermined amount (2 mg / cm 2) of the arms of 20 subjects respectively and rubbed well. The moisture increase rate (%) at 1 hour and 3 hours after the measurement was measured to evaluate the moisturizing effect.

As a result, the essence containing the extract of Fermented Red Ginseng of the present invention showed significantly higher moisture retention after 1 hour and 3 hours than the essence containing the extract of Comparative Examples 1 and 2, (Table 3).

division Moisture holding power (A.U.) Example 1 Comparative Example 1 Comparative Example 2 Before application 0 0 0 After 1 hour of application 225 175 184 After 3 hours of application 198 161 167

< Manufacturing example  1> Cosmetics  Preparation of composition

<1-1> Preparation of essence

The extracts prepared in Example 1 and Comparative Examples 1 and 2 were used to prepare essences according to the composition (% by weight) shown in Table 1 below.

Furtherance Example 1 Comparative Example 1 Comparative Example 2 extract 3 3 3 Triethanolamine 0.25 0.25 0.25 Carboxyvinyl polymer 0.22 0.22 0.22 glycerin 4 4 4 Butylene glycol 2 2 2 Paraoxybenzoic acid ester 0.2 0.2 0.2 Wax 0.5 0.5 0.5 Cetostearyl alcohol One One One Glyceryl monostearate One One One Squalane 4 4 4 Purified water Suitable amount Suitable amount Suitable amount

<1-2> Production of softening longevity

The softened lotion containing the ginseng fermented extract of the present invention as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) The red ginseng fermented extract of Example 1 1.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<1-3> Preparation of nutritional cream

The nutritional cream containing the ginseng fermented extract of the present invention as an active ingredient was prepared in accordance with the composition shown in Table 6 below.

Raw material Content (parts by weight) The red ginseng fermented extract of Example 1 1.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

<1-4> Production of Lotion

Lotions containing the ginseng fermented extract of the present invention as an active ingredient were prepared as shown in the composition of Table 7 below.

Raw material content( Weight portion ) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 FAGE -100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethylparaben 0.1 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethylparaben 0.1 Propylparaben 0.1 1,3-butylene glycol 4 Methylparaben 0.1 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 Allantoin 0.1 The red ginseng fermented extract of Example 1 1.0 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 pt 41891 0.1 p-H20 48.3

< Manufacturing example  2> Preparation of pharmaceutical composition

<2-1> Preparation of ointment for external skin

Skin ointments containing the ginseng fermented extract of the present invention as an active ingredient were prepared according to the composition shown in Table 8 below.

Raw material content( Weight portion ) Purified water 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 The red ginseng fermented extract of Example 1 1.0 Caprylic capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Wax 4.0

<2-2> External application Patchy  Produce

The skin external patches containing the ginseng fermented extract of the present invention as an active ingredient were prepared according to the composition shown in Table 9 below.

Raw material Content (parts by weight) The red ginseng fermented extract of Example 1 1.0 Hexylene glycol 20.0 Diethylamine 0.7 Polyacrylic acid 1.0 Sodium sulphate 0.1 Polyoxyethylene lauryl ether (E.0 = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether 1.0 Viscous paraffin oil 2.5 Caprylic acid ester / capric acid ester (cetiol LC) 2.5 Polyethylene glycol 400 3.0 Deionized water 70

<2-3> Sanje  Produce

2 g of fermented red ginseng extract of Example 1

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<2-4> Preparation of tablets

100 mg of fermented red ginseng extract of Example 1

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 2-5 > Preparation of capsules

100 mg of fermented red ginseng extract of Example 1

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

&Lt; 2-6 >

1 g of fermented red ginseng extract of Example 1

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

<2-7> Preparation of granules

150 mg fermented red ginseng extract of Example 1

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and the mixture was dried at 60 캜 to form granules, which were then filled in a capsule.

< Manufacturing example  3> Manufacture of health food

<3-1> Production of flour food

0.5 to 5.0 parts by weight of the ginseng fermentation extract of the present invention was added to wheat flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture.

<3-2> soup  And juicy ( gravies )

0.1 to 5.0 parts by weight of the ginseng fermentation extract of the present invention was added to soups and juices to prepare health promotion meat products, noodle soups and juices.

<3-3> Dairy products ( dairy products )

5 to 10 parts by weight of the ginseng fermented extract of the present invention was added to milk and various dairy products such as butter and ice cream were prepared using the milk.

<3-4> Health drink  Produce

The ginseng fermented extract of the present invention (5 g) was homogenously mixed with a supplementary ingredient such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% And then packaged in small containers such as glass bottles and plastic bottles to produce beverages

Claims (9)

A cosmetic composition for prevention of skin aging and skin moisturization, which contains, as an active ingredient, ginseng fermented extract prepared by fermenting ginseng extract with a strain of Pichia ciferrii. The method according to claim 1,
Wherein the ginseng is at least one selected from the group consisting of red ginseng, ginseng ginseng, Chinese ginseng, and jelly ginseng. The method according to claim 1,
The ginseng extract may be selected from the group consisting of water, anhydrous or lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol as an extraction solvent, A cosmetic composition for moisturizing improvement. The method according to claim 1,
The ginseng extract is extracted with ethanol as an extraction solvent, and is an ethanol-soluble extract, which is a cosmetic composition for preventing skin aging and skin moisturization. The method according to claim 1,
Wherein the content of the ginseng fermentation extract is 0.0001 to 99.0% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the cosmetic composition is any one selected from the group consisting of external ointment for skin, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion and oil gel. The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta agent and a cataplasma agent. . delete A health food composition for preventing skin aging and improving skin moisturization, which contains ginseng fermented extract prepared by fermenting Ginseng Extract with Pichia peperii as an active ingredient.