WO2005030235A1

WO2005030235A1 - Method of processing ginseng for medicinal use and composition

Info

Publication number: WO2005030235A1
Application number: PCT/JP2004/014265
Authority: WO
Inventors: Yuusuke Mori; Naoaki Morihara; Takahiro Yamakawa; Toshinobu Morita; Kazuhiko Imamura
Original assignee: Wakunaga Pharmaceutical Co., Ltd.
Priority date: 2003-09-30
Filing date: 2004-09-29
Publication date: 2005-04-07
Also published as: CN100586444C; JP4767686B2; JPWO2005030235A1; HK1096042A1; CN1859921A

Abstract

It is expected that the effects of ginseng for medicinal use, i.e., a liver function-improving effect, a hemostatic effect, an antistress effect, an antitumor effect, an aging-retarding effect, an antioxidative effect and so on can be enhanced by a processing method comprising the step of contacting a ginseng plant or its extract with a microorganism or an enzyme and the step of reacting it with an acid or an alkali. The thus processed product is usable as foods, drugs, cosmetics, etc.

Description

Specification

Ginseng processing method and composition

Technical field

The present invention relates to a method for processing ginseng and a composition obtained by the processing. Priority is claimed on Japanese Patent Application No. 2003-339752, filed on September 30, 2003, the content of which is incorporated herein by reference.

Background art

[0002] In recent years, due to changes in lifestyles, especially dietary habits (eg, increased intake of fatty foods, smoking and drinking alcohol, etc.), fat and stress have accumulated in the body, and liver function has been reduced. The so-called lifestyle-related diseases, such as hyperlipidemia, hypertension, and gout, have rapidly increased and become social problems. These illnesses are accompanied by complications as the disease progresses, and shift to higher-ranking diseases such as heart disease and stroke. For this reason, its treatment and prevention is extremely important. For these diseases, several therapeutic agents have been developed, such as synthetic drugs. However, if there are concerns about side effects and other complications, it is necessary to take more than one drug, which may lead to serious side effects. Therefore, there is a demand for a material which has a therapeutic or preventive effect and is highly safe against these diseases or complications.

[0003] One of the materials that can meet these demands is ginseng. Ginseng has been used in China since ancient times and is widely used as a Chinese medicine. Ginseng is known to have a medicinal and preventive effect on arteriosclerosis, hyperlipidemia, thrombosis, hypertension, decreased immune function, decreased liver function, tumor, hyperglycemia, stress, and the like. However, their effects were not fully satisfactory.

[0004] As a method for solving these problems, processing of ginseng may be considered. For example, a method of fermenting ginseng with yeast (for example, Patent Document 1) has been reported. However, this method was aimed at improving the flavor, and the effect of improving the efficacy was insufficient. A method has also been reported in which red ginseng extract is made to react with sugar hydrolase (for example, Patent Document 2), but it cannot be said that sufficient medicinal effects have been obtained, and there is still room for improvement. Was.

Patent Document 1: Japanese Patent Application Laid-Open No. 3-277246

Patent Document 2: Japanese Patent Application Laid-Open No. 2002-348245

Disclosure of the invention

Problems to be solved by the invention

[0006] An object of the present invention is to provide a processing method for improving the efficacy of ginseng and a composition obtained by the processing.

Means for solving the problem

[0007] The inventors of the present invention have conducted intensive studies based on the above-described problems, and as a result, have been found to include a step (a) of bringing ginseng into contact with a microorganism or an enzyme and a step (b) of reacting the same with an acid or an alkali. The present inventors have found that the effect of ginseng is significantly improved by performing ginseng, and completed the present invention.

[0008] That is, the present invention includes a step (a) of bringing ginseng into contact with a microorganism or an enzyme, and a step (b) of reacting ginseng with an acid or an alkali. The present invention relates to a method for processing ginseng, wherein the processed ginseng is further processed in the remaining steps after the processing.

[0009] Further, in the present invention, a step (a) of bringing ginseng into contact with a microorganism or an enzyme and a step (b) of reacting ginseng with an acid or an alkali are carried out in this order for processing. It relates to a method of processing ginseng.

[0010] The present invention also relates to a method for processing ginseng, wherein the step (a) may be a step of contacting with an enzyme, and the step (b) may be a step of reacting with an acid.

[0011] The present invention also relates to a composition obtained by a method for processing ginseng, comprising a step (a) of contacting with a microorganism or an enzyme and a step (b) of reacting with an acid or an alkali. .

Further, the present invention provides a food containing the above composition.

The invention's effect

[0013] The ginseng processing method of the present invention does not require special operations and facilities. Also, for processing The resulting composition exhibits an extremely high hepatoprotective effect (improving hepatic function), anti-atherosclerosis, anti-aging effects, etc., and thus can be widely used as foods, pharmaceuticals and cosmetics. Monkey

BEST MODE FOR CARRYING OUT THE INVENTION

An object of the present invention is to provide a processing method for improving the efficacy of ginseng and a composition obtained by the processing. The ginseng used in the present invention includes seven ginseng (Panax notoginseng (Burk.) FH and hen), t¾reijin (Panax ginseng and A. Meyer), bamboo ginseng (Panax japonicus CAMeyer), and American ginseng (Panax quinquefolium L.), Vietnamese ginseng (Panax vietnamensis Ha et Grushv.), Ginger-like Nanachi (Panax zingiberensis CYWu et KMFeng), Sanax Fangbe (Panax stipuleanatus HTTsai etK.M.Feng) and Himasha ginseng (Panax pseudo— ginsengWall. subsp. himalaicus Hara) Ginseng and rice ginseng are preferred, and rice ginseng is particularly preferred. These ginseng can also be used in combination of two or more.

[0015] As the site of use of the ginseng used in the present invention, the whole plant can be used. For example, roots, rhizomes, stems, branch roots, whiskers, tuberous roots, leaves, flowers, fruits, seeds, buds, baskets, etc. can be used alone or in combination, and preferably roots or rhizomes can be used.

. In addition, the plant can be used after crushing, crushing, repairing, cutting, roasting, and processing such as Z or drying.

The ginseng used in the present invention or the ginseng processed in the step (a) and step Z or step (b) according to the present invention is obtained by extracting a plant with a polar solvent or edible oil. The obtained extract, its diluted solution, concentrated solution, extract, and dried product obtained by drying it may be used. Examples of polar solvents used for extraction include water; alcohols such as methanol, ethanol, 1-propanol, 2-propanol, and 1-butanol; ethers such as 1,4-dioxane; ketones such as acetone; Tolyls; mixtures of these and the like. Preferred are water, methanol, ethanol and mixtures thereof, particularly preferably water, ethanol and mixtures thereof. Examples of edible oils used for the extraction include rapeseed oil, castor oil, salad oil, soybean oil, olive oil, corn oil, sesame oil, safflower oil, flax oil, perilla oil, perilla oil, and tung oil. [0017] Ginseng extract can be produced by a method generally known in the art, for example, by adding the above-mentioned polar solvent to a plant or its dried product obtained by pulverizing, crushing or cutting the plant. At 100 ° C, preferably 20-70 ° C for 10 minutes to 24 hours, preferably 30 minutes to 10 hours.If necessary, remove insolubles by filtration, centrifugation, etc., and concentrate. Can be performed. Further, if necessary, it can be purified by washing with a non-polar solvent such as ethyl acetate, getyl ether and hexane, and extracting with water.

[0018] The reaction with a microorganism in the processing method of the present invention can be performed by a generally known method. For example, a microorganism or a microorganism previously cultured in an appropriate medium, a ginseng plant, an extract thereof, or a product obtained by processing the same in step (b), or a product obtained by dissolving or suspending the extract in water , Etc. can be mixed and reacted. At this time, carbohydrates (glucose, sucrose, etc.) and proteins (rice bran, bran, etc.) may be added as needed. The temperature during the reaction is not particularly limited as long as it is within the activity temperature range of the microorganism. 10-50 ° C is preferred 20-40 ° C is more preferred. After completion of the reaction, if necessary, the target substance can be obtained by removing the insolubles by heat treatment and operations such as Z or filtration or centrifugation and concentrating or drying.

Examples of the microorganism used for the reaction with the microorganism in the processing method of the present invention include, for example, Ashergillus genus (eg, Aspergillus japonicus, Aspergillus flavus, Aspergillus wentu, Aspergillus niger, Aspergillus niger awamori, Aspergillus oryzae, Aspergillus pulverlentus and the like) ), Notochinoles (f column, Bacillus acidopullalyticus, Bacillus

amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus circulans, Bacillus licheniformis, Bacillus polymyxa, etc.), Fukutonoku Shinoresu genus ([Retsue is, Lactobacillus brevis, Lactobacillus pentoaceticus, Lactobacillus pastorianus, Lactobacillus Lactobacillus casei, Lactobacillus cucumeris, Lactobacillus bulgaricus , Lactobacillus delbruecku, Lactobacillus jugurti ゝ Lactobacillus helveticus,

Lactobacillus plantrum, Lactobacillus acidophilus and the like, and microorganisms such as lactic acid bacteria, yeast, and filamentous fungi belonging to the genus Rhizopus (eg, Rhizopus delemar, Rhizopus oryzae) and the like. Aspergillus, Bacillus and Lactobacillus are preferred. Is particularly preferred. In the genus Aspernoreginoles, Aspergillus niger, Aspergillus niger awamon, and Aspergillus oryzae 3 are preferred, and Aspergillus oryzae is particularly preferred. These microorganisms may be used alone or in combination.

[0020] The enzyme reaction in the processing method of the present invention can be performed by a generally known method.

For example, ginseng plants, their extracts, or those obtained by processing the same in step (b), or those obtained by dissolving or suspending the extracts in water, require the required amount of enzymes, such as ginseng. 0.001 to 1% by weight, preferably 0.05 to 0.3% by weight, and with appropriate stirring 0 to 90 ° C, preferably 20 to 70 ° C, more preferably 30 to 50 ° C Leave for 1 hour and 1 week, preferably 5 hours and 5 days. Thereafter, the enzyme is inactivated by a heat treatment, and if necessary, for example, filtration, Z or centrifugation is performed to remove insolubles, followed by concentration or drying to obtain the desired product.

Examples of the enzymes used in the enzymatic reaction in the processing method of the present invention include, for example, senorylase, hemicenorelase, xylanase, lactase, pectinase, protease, α-galactosidase, j8-galactosidase, α-amylase, β-amylase. Examples include amylase, hesperidinase and naringinase. Preferred are ratatase, xylanase, cellulase, hemicellulase, j8-galactosidase and pectinase, and particularly preferred is | 8-galactosidase. These enzymes can be used in combination of two or more depending on the reaction temperature.

[0022] The reaction with an acid or alkali in the processing method of the present invention can be performed by a generally known method. For example, a ginseng plant, an extract thereof, or a product obtained by processing the same or an extract thereof in step (a) may be used at a concentration of 0.001 to 50%, preferably 0.5 to 10%. Dissolve or suspend in acid or alkali. Thereafter, heat treatment is performed at 80 to 150 ° C, preferably 100 to 130 ° C, for 5 minutes to 14 hours, preferably for 30 minutes to 12 hours. After that, if necessary, the desired product can be obtained by removing insolubles by removing the solvent by operations such as neutralization, filtration and Z or centrifugation, removing the solvent, and concentrating or drying.

[0023] The acid or alkali used for the acid or alkali reaction in the processing method of the present invention is not particularly limited. Examples of the acid include inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, and nitric acid, acetic acid, citric acid, phosphoric acid, malic acid, succinic acid, fumaric acid, tartaric acid, lactic acid, and vinegar (for example, Vinegar and synthetic vinegar such as grain vinegar and fruit vinegar). Preferred are hydrochloric acid, acetic acid, citric acid and vinegar. These acids may be used alone or in combination of two or more. Examples of the alkali include alkalis such as sodium hydroxide, potassium hydroxide, sodium hydrogen carbonate, and sodium dihydrogen phosphate. Preferred are sodium hydroxide and potassium hydroxide. These alkalis may be used alone or in combination of two or more. In the reaction with an acid or an alkali reaction in the processing method of the present invention, the pH of the acid is preferably 1.0 to pH <4.5 in the reaction with an acid, and more preferably 2.0 <pH <4. 0, and more preferably 2.0 <pH 3.8. In addition, in the reaction with anorecali, the pH of anorecali is preferably 7.5 to pH 11.0, more preferably 8.0 <pH <10.0, and further preferably 8.5 <pH <10.0.

The method for processing ginseng of the present invention includes a step (a) of bringing ginseng into contact with a microorganism or an enzyme, and a step (b) of reacting ginseng with an acid or an alkali. The order can be arbitrarily selected, but it is preferred that the step (a) of bringing ginseng into contact with a microorganism or an enzyme and the step (b) of reacting with an acid or an alkali are sequentially reacted in this order. In each step, the reaction with an enzyme is preferred in step (a), and the reaction with an acid is preferred in step (b) from the viewpoint of improving the medicinal effect of ginseng. For this reason, the reaction is more preferably performed sequentially in the order of the step of contacting with the enzyme and the step of reacting with the acid.

[0025] The composition obtained by the processing method of the present invention can be used after further extraction and purification with Z or a column. The extraction can be performed by the above method. For column purification, for example, after dissolving or suspending the composition of the present invention in purified water, it is added to a column of a giant reticulated resin, and unnecessary substances are washed with purified water, followed by 70-90% The desired product can be obtained by eluting with ethanol and concentrating the eluate. If necessary (for example, for decolorization), the ethanol eluate may be passed through an ion exchange resin column, and then eluted with 70-90% ethanol. The composition of the present invention can be obtained by concentrating the eluate.

[0026] The giant reticulated resin used in the present invention is, for example, a porous polymer polymerized with styrene, diphenylstyrene or a_methylacrylic ester, and has a large surface area. It is the one in which fine continuous pores called pores develop into the inside of polymer particles. It is known from this structural feature that it efficiently adsorbs organic substances, and is used for concentration and purification of natural products and fermentation products. As the giant reticulated resin, a generally known resin is used. For example, Amberlite XAD-1 and Amberlite XAD-2 (all manufactured by OM & Haas Co., Ltd.), DIAION HP20, DIAION HP21, Sepabeads SP 825, Seno 《Beads SP850, Seno 《Beads SP207, DIAION HP2MG (Above, manufactured by Mitsubishi Chemical Corporation) and the like.

[0027] The ion exchange resin used in the present invention does not require a special one, and a generally known ion exchange resin can be used. Examples of the ion exchange resin include a strongly acidic cation exchange resin, a weakly acidic cation exchange resin, a strongly basic anion exchange resin, a weakly basic anion exchange resin, and a mixed resin. Fats, weakly acidic cation exchange resins and mixed resins.

[0028] The composition obtained by the processing method of the present invention, an extract thereof, or a purified product of a column has various medicinal effects (preventive and therapeutic effects)! For example, hepatoprotective effect (effect of improving liver function), anti-atherosclerosis, anti-aging, anti-tumor effect, anti-hyperlipidemia, anti-thrombosis, blood pressure lowering effect, immune function improvement, blood sugar lowering effect, analgesic effect, inotropic effect It has an anti-inflammatory effect, a peripheral blood flow improving effect, a hemostatic effect, an antioxidant effect, an anti-stress effect and the like. Therefore, it may be used for their treatment. The composition of the present invention, the extract thereof, and the purified product of the column can be used in combination with each other. Also, a plurality of compositions obtained by the method of the present invention may be used in combination!

[0029] The composition obtained by the processing method of the present invention, an extract thereof, or a purified product of a column can be used as a food, drug, cosmetic or oral composition (dentifrice) according to the intended use.

When used as food, other food materials can be blended with the composition of the present invention. For example, when the purpose is to provide a hepatoprotective effect (improving hepatic function), it is necessary to use pulp polishes, konkon, mariazami, nutmeg, lycopodium, isoflavone, royal jelly, lactoferrin and its extract, liver extract, etc. Can be blended. If it is intended to provide an antioxidant effect, use astaxanthin, grape seeds (proantasia ), Vitamin E, polyphenol, power techin and the like. For the purpose of imparting anti-arteriosclerosis, licorice, peony, oak, ginseng, grape seeds (proanthocyanin), linseed seeds, and the like and extracts thereof can be blended. For the purpose of giving antithrombosis, nutrient kinase, linseed seeds and the like and extracts thereof can be combined. For the purpose of lowering the blood sugar level, vanapa, salsia blolonga, salsia reticularata, white sweet potato, yacon, tonpuri, reishi and its extract, procya-gin and the like can be added. For the purpose of imparting blood pressure lowering effect, use γ-aminobutyric acid, sardine peptide, casopeptide, tuna peptide, propolis, kale, reishi, nose, grape seed (proanthocyanin), etc. and extracts thereof Can be blended. These compounds may be used alone or in combination of two or more.

The food materials that can be blended in the composition of the present invention are not limited to the above-mentioned materials, and can be appropriately blended according to the use and purpose thereof. Excipients can be added as needed.For example, cellulose, crystalline cellulose, lactose, sucrose fatty acid ester, lactose, reduced maltose, pregelatinized starch, pine fiber, dextrin, no index, etc. can be appropriately added. Can be.

[0030] The form of the food can be, for example, a solid food, a creamy or jam-like semi-liquid food, a gel-like food, a tablet, a powder, a caplet, and a soft capsule or a hard capsule. In this case, when the capsule film is gelatin, for example, in order to prevent insolubilization of the film, phosphatidylethanolamine and soybean lecithin and egg yolk lecithin containing the phosphatidylethanolamine are added to the total content of the capsule. Or an amino sugar (a saccharide having an amino group such as darcosamine, Ν-acetyl darcosamine, galactosamine, Ν-acetylgalactosamine, Ν-acetylneuraminic acid, etc., is chitosan which is a polymer of dalcosamine) 0.05 to 20 mass ^_0/0, (inositol phosphate derivatives such as phytic acid, and Kuen acid) organic acid 0.5 05 20 wt%, it may be blended.

[0031] Soft capsules containing the composition obtained by the processing method of the present invention, an extract thereof, or a purified product of a column as a filling content can be produced by a generally known method. For example, gelatin may be dissolved in water (at this time, heat may be added. Preservatives, amino acids and the like can be added. ), The resulting solution is dried to a moisture content of 5-20%, more preferably 7-15% to obtain a capsule shell. The filled contents obtained by adding the composition of the present invention, soybean lecithin, phytic acid and, if necessary, an excipient to the capsule film are added in the form of liquid, suspension, paste, powder or granules. It can be manufactured by filling or encapsulating the filling content with the above capsule film.

The composition of the composition obtained by the processing method of the present invention, the extract thereof, or the purified product of the column can be arbitrarily selected in the amount contained in food.

[0032] When the composition obtained by the processing method of the present invention, an extract thereof or a purified product of a column is used as a cosmetic, the cosmetic is used in accordance with the intended use within a range that does not impair the effects of the present invention. And 0.00000 to 10.0% by weight, preferably 0.00001 to 5.0% by weight, based on the total weight of the cosmetic. Further, if necessary, vitamin C, vitamin E, astaxanthin, grape seed (proanthocyanin), collagen, chondroitin, dalcosamine and the like can be appropriately selected and used. These may be used alone or in combination of two or more.

When a composition obtained by the processing method of the present invention, an extract thereof or a purified product of a column is used as a shampoo component, the ion content may be adjusted within the range not impairing the effects of the present invention in accordance with the intended use. Surfactants, nonionic surfactants, foaming agents, thickeners, powders, scrubbing agents, antioxidants, preservatives, oils, wetting agents, ultraviolet absorbers, polymers, inorganic pallets Ingredients commonly used in cosmetics, such as anti-dandruff agents, chelating agents, pH adjusters, electrolytes, coloring agents, fragrances, cosmetic ingredients for scalp or hair care, etc. can be blended. These components may be used alone or in combination of two or more. The amount of the composition obtained by the processing method of the present invention, the extract thereof, or the purified product of the column contained in the shampoo can be arbitrarily selected.

[0034] When the composition obtained by the processing method of the present invention, its extract or purified column is used as a component of an oral composition (such as a dentifrice), an abrasive, a wetting agent, a binder, Blowing agents and other components generally used in oral compositions (dentifrices) can be blended. These components may be used alone or in combination of two or more. When the composition obtained by the processing method of the present invention, an extract thereof, or a purified column is used as an oral composition, the amount of the composition contained therein can be arbitrarily selected.

When the composition obtained by the processing method of the present invention, its extract or purified column is used as a medicament, injection, parenteral administration such as rectum, oral administration in solid or liquid form, etc. Can be formulated as a composition with a pharmaceutically acceptable carrier. These components may be used alone or in combination of two or more. The amount of the composition, extract or column purified product obtained by the processing method of the present invention contained in the drug can be arbitrarily selected.

[0036] The form of the composition according to the present invention for injection may include pharmaceutically acceptable sterile water, non-aqueous solution, suspension or emulsion. Examples of suitable non-aqueous carriers, diluents, solvents or vehicles that can be used above include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl ethyl oleate. Compositions for such injections may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents. These compositions are mixed with a sterilizing agent, for example by filtration through a bacteria-retaining filter, or just prior to use, in the form of a sterile solid composition that can be dissolved in sterile water or some other sterile injectable medium. By doing so, it can be sterilized.

[0037] Examples of solid preparations for oral administration include capsules, tablets, pills, powders, and granules. In preparing this solid preparation, the compound of the present invention is generally mixed with at least one inert diluent, for example, sucrose, lactose, starch and the like. The formulation may further comprise additional substances other than inert diluents, such as lubricants (eg, magnesium stearate, etc.) in the usual formulation. In the case of capsules, tablets and pills, a buffer may also be included. Tablets and pills are further provided with an enteric coating.

[0038] Liquid preparations for oral administration include inert diluents commonly used by those skilled in the art, such as pharmaceutically acceptable emulsions, solutions, suspensions, syrups and elixirs containing water. Is mentioned. In addition to powerful inert diluents, the compositions can also include adjuvants such as wetting agents, emulsifying, suspending, sweetening, flavoring, and flavoring agents. For rectal administration In the case of pharmaceutical preparations, it is preferable to contain excipients such as cocoa butter and suppository wax in addition to the compound of the present invention.

[0039] The dose of the composition obtained by the processing method of the present invention, its extract or purified product of the column depends on the composition to be administered, the properties of the extract or purified product of the column, the administration route, and the desired treatment period. And other factors. Generally, about 0.1-100 mg / kg per day, especially about 0.5-50 mg / kg, is preferred. If desired, the daily dose can be divided and administered in 2 to 4 times.

Example

Hereinafter, the present invention will be described by way of examples, but these do not limit the present invention. Example 1

[0041] 100 g of Nanatsu ginseng powder and 10 g of an enzyme agent ratatase F "Amano" (manufactured by Amano Enzym Co., Ltd.) derived from Aspergillus oryzae were weighed, and 2.5 L of water was added thereto to dissolve and suspend. The mixture was placed in an incubator at ° C and reacted for 5 days with occasional shaking. Then, acetic acid was added to a concentration of 1νΖν% acetic acid, and the mixture was reacted by heating (120 ° C, 40 minutes). At the same time, the enzyme was inactivated, and the reaction solution was freeze-dried to obtain 113 g of brown powder. Obtained. Example 2

[0042] 100 g of ginseng and 10 g of an enzyme agent ratatase F "Amano" (manufactured by Amano Enzym Co., Ltd.) derived from Aspergillus oryzae, 2.5 L of water are added thereto, and the mixture is dissolved and suspended. And allowed to react for 5 days with occasional shaking. Then, acetic acid is added so that the concentration becomes ΙνΖv% acetic acid, and the mixture is reacted by heating (120 ° C, 40 minutes). At the same time, the enzyme is deactivated. 98 g were obtained.

Example 3

[0043] Measure 100g of Tanashi Ginseng powder and 5g of Cellulase Y-NC (manufactured by Yakult Yakuhin Kogyo Co., Ltd.) from Aspergillus niger, add 2.5L of water thereto, and incubate at 50 ° C. The reaction was continued for 5 days with occasional shaking. Thereafter, 10 mL of 5% hydrochloric acid was added, and the mixture was heated (120 ° C., 40 minutes) to cause a reaction, and at the same time, the enzyme was inactivated. This reaction solution was freeze-dried to obtain 108 g of a brown powder. Example 4

[0044] 10 g of cell mouth Shin HC (manufactured by Hankyu Bio-Industry Co., Ltd.), an enzyme agent derived from lOOg and Aspergillus niger, is measured, 2.5 L of water is added thereto, and the incubator is heated to 50 ° C. The mixture was put in one and allowed to react for 5 days with occasional shaking. Thereafter, 5 g of citrate was added, and the mixture was heated (120 ° C., 40 minutes) to react, and at the same time, the enzyme was inactivated. This reaction solution was freeze-dried to obtain 115 g of a brown powder.

Example 5

[0045] 5. Og of the powder obtained in Example 1 is dissolved in 100 mL of purified water. This solution is added to a column of Diaion HP20 (manufactured by Mitsubishi Iridaku Co., Ltd.) 200CC, and unnecessary substances are washed away with 10 times the column volume of purified water. Then elute with 5 times the column volume of 70% ethanol. The eluate is collected and passed through ion-exchange resin “Diaion SK104” (manufactured by Mitsubishi Iridaku Co., Ltd.), and then eluted with 70% of the same column volume of ethanol. The eluate was concentrated and dried under reduced pressure to obtain 400 mg of a solid powder.

(Test Example 1)

In order to compare the hepatoprotective effect of the composition obtained in Example 1 with untreated ginseng, an experiment was performed using a galactosamine-induced liver injury model in rats. This galactosamine-induced liver injury model is known as a general immunological liver injury model in consideration of clinical correlation. When hepatocytes are damaged, enzymes such as aspartate aminotransferase (GOT) and peranine aminotransferase (GPT) in the cells are released into the blood, and the activity of these enzymes rapidly increases. That is, since these GOT and GPT activities in blood are known as indicators of liver dysfunction, the following experiments were performed.

Wistar male rats (6 weeks old) were used for the experiment after pre-breeding for 1 week. The experimental groups consisted of four groups: a normal group, a control group, and seven ginseng fields. 3.OgZkg-administered group and a group treated with seven ginseng fields (Example 1). Each group consisted of 10 animals. All administration solutions were orally administered to rats in a volume of 20 mLZkg, and the control group was orally administered the same amount of distilled water. Except for the normal group, the test substance was orally administered to the three groups once a day for 7 days, and 400 mg Zkg of galactosamine was intravenously administered on the last day for liver injury. Was caused. Blood was collected from the abdominal aorta 24 hours after the administration of extra-gala samin, and the GPT and GOT activities in the plasma were measured using a transaminase CII test kit. The test was performed by one-way ANOVA followed by Tukey's multiple test. Figures 1 and 2 show the results. The treated product of Example 7 was significantly reduced in GPT and GOT activities (p <0.01) as compared to that of the field, and was found to have a hepatoprotective effect. Was.

(Example of soft capsule production)

A gelatin solution consisting of 43% by weight of gelatin, 17% by weight of glycerin and 40% by weight of water is dissolved and degassed at 80 ° C, and then left standing for about 10 hours to be filled with a rotary soft capsule filling machine (Oval 5 type). ), 180 mg of perilla oil in which 150 mg of the powder of Example 1 was dissolved, 2 mg of soybean lecithin, and 18 mg of phytic acid were filled. After filling the capsules, the capsules were dried at a temperature of 27 ° C and a humidity of 50% or less to a film moisture of 8%. Soft capsules were produced.

(Reference Example 1)

100 g of the field ginseng powder was weighed out, and 2.5 L of water was added thereto to dissolve and suspend. Further, acetic acid was added to a concentration of lv Zv% acetic acid, and the mixture was reacted by heating (120 ° C., 40 minutes), and the reaction solution was freeze-dried to obtain 101 g of a brown powder.

[0049] (Reference Example 2)

Weigh 10 g of Latase F “Amano” (manufactured by Amano Enzym Co., Ltd.), 100 g of Asagillus oryzae, and dissolve and suspend 2.5 g of water in the incubator at 50 ° C. And allowed to react for 5 days with occasional shaking. Thereafter, the enzyme was inactivated by heat treatment with calo (105 ° C, 10 minutes), and the reaction solution was freeze-dried to obtain 110 g of a brown powder.

(Test Example 2)

In order to examine the hepatoprotective effect of the composition obtained in Example 1 and the raw rice ginseng, Reference Example 1 composition and Reference Example 2 composition, a rat galactosamine-induced liver injury model and tetrasalt An experiment was performed using a dani carbon-induced liver injury model. It is known that the extra-gala samin-induced liver injury model is a viral hepatitis model, and the Shishi-dani carbon-induced liver injury model is an toxic hepatitis model, which has high correlation with clinical results.

7-week-old male Wistar rats (6 or 7 weeks old) Was used for a galactosamine-induced liver injury model, and a rat aged 8 weeks was used for a Shishi-dani-induced liver injury model experiment. The experimental group was a normal group, a control group, a raw ginseng 3.OgZ kg administration group, Reference Example 1 composition 3.OgZkg administration group, Reference Example 2 composition 3.3gZkg administration group and Example 1 composition 3. There were 6 groups of 3gZkg administration group, 3 groups for normal group and 10 groups for other groups. All administration solutions were orally administered to rats in a volume of 20 mLZkg, and the control group was orally administered the same amount of distilled water. In an experiment using a galactosamine-induced liver injury model, the test substance was orally administered once daily for 4 days to 5 groups except the normal group, and 400 mg Zkg of galactosamine was intravenously administered on the last day to induce liver injury. Was. Similarly, in an experiment using the Shisho-Dani-Carbon-induced liver injury model, the test substance was orally administered once daily for 5 days to five groups except the normal group, and on the last day, 0.75 mL Was administered intraperitoneally to induce liver damage. Twenty-four hours after the administration of extra-gala samin (or tetrashidani carbon), blood was collected from the abdominal aorta and the GPT activity in the plasma was measured using a transaminase CII test. The test was performed by one-way ANOVA followed by Tukey's multiple test. X when no significant difference is observed compared to control group, X when significant difference is observed (p <0.05), and (§) (p <0 01) and the results are shown in Table 1. Compared with the raw rice ginseng, the composition of Reference Example 1, and the composition of Reference Example 2, the composition of Example 1 showed a strong GPT activity increase inhibitory effect in both models. From the above, it was confirmed that the composition of Example 1 had a strong hepatoprotective effect.

[Table 1]

Industrial applicability

[0052] According to the present invention, various pharmacological effects having medicinal ginseng can be enhanced. Brief Description of Drawings

FIG. 1 is a graph comparing the effects of hepatoprotection on GPT activity in an acute liver injury model induced by galactosamine.

FIG. 2 is a graph comparing the effects of hepatoprotective action on GOT activity in an acute liver injury model induced by galactosamine.

Claims

The scope of the claims

[I] a step of contacting ginseng with a microorganism or an enzyme, and a step of reacting ginseng with an acid or alkali (b),

A method for processing ginseng, comprising treating ginseng in one of the above steps and then treating the treated ginseng in the remaining steps.

[2] The method for processing a ginseng according to claim 1, wherein the step (a) of contacting the ginseng with a microorganism or an enzyme and the step (b) of reacting the ginseng with an acid or an alkali are processed in this order.

[3] The method for processing ginseng according to claim 1, wherein the step (a) is a step of contacting the ginseng with an enzyme, and the step (b) is a step of reacting the ginseng with an acid.

[4] The method for processing ginseng according to claim 1, wherein the ginseng is seven ginseng.

[5] A composition obtained by the processing method according to claim 1.

[6] A composition obtained by purifying the composition according to claim 5 by at least one of extraction and column.

[7] A food containing the composition according to claim 5.

[8] A medicament comprising the composition according to claim 5.

[9] The medicament according to claim 8, which is a hepatoprotective agent.

[10] A cosmetic, comprising the composition according to claim 5.

[II] A soft capsule containing the composition according to claim 5.

[12] The composition according to claim 5, wherein the composition has a hepatoprotective effect, anti-atherosclerosis, anti-aging, anti-tumor effect, anti-hyperlipidemia, anti-thrombosis, blood pressure lowering effect, immune function improvement, blood glucose lowering effect, analgesia. A composition exhibiting at least one selected from the group consisting of action, cardiotonic action, anti-inflammatory action, peripheral blood flow improving action, hemostatic action, antioxidant action, and anti-stress.

[13] A therapeutic method using the composition according to claim 5.