CN103446208B - A kind of method that ginseng pulp is prepared using cellulase - Google Patents
A kind of method that ginseng pulp is prepared using cellulase Download PDFInfo
- Publication number
- CN103446208B CN103446208B CN201310355912.6A CN201310355912A CN103446208B CN 103446208 B CN103446208 B CN 103446208B CN 201310355912 A CN201310355912 A CN 201310355912A CN 103446208 B CN103446208 B CN 103446208B
- Authority
- CN
- China
- Prior art keywords
- ginseng
- pulp
- cellulase
- value
- hydrolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods that ginseng pulp is prepared using cellulase hydrolysis, this is included the following steps using the method that cellulase hydrolysis prepares ginseng pulp：Ginseng is taken to be cut into blocky or sheet, adds in pure water；It is rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjusts pH value, uses autoclaving equipment boiling ginseng pulp；Ginseng pulp pH value is adjusted before enzymolysis, is heated in 50 DEG C of constant temperature water baths；In the cellulase to ginseng juice for adding 800~2500 g tons, digest 1 hour, then carry out centrifugal filtration processing；Enzymolysis supernatant temperature is adjusted, carries out destroy the enzyme treatment 15~30 minutes, obtains the ginseng pulp of cellulosic sections hydrolysis.The present invention is widely used, and simple production process solves the problems, such as that the conventional ginseng processing technology production cycle is long, production equipment is costly.In addition, the efficient active ingredient obtained in ginseng of the present invention, ingredient the effect of effective protection ginseng, and promote the process of processing of Panax ginseng technological innovation.
Description
Technical field
Preparing technical field more particularly to a kind of side that ginseng pulp is prepared using cellulase the invention belongs to ginseng pulp
Method.
Background technology
Ginseng is the common medicinal material in Asia, and also generally using American ginseng, many herbal medicine pavings and supermarket can find in northern Central America
Various ginseng medicine materical crude slice and extract health product, for restoring, enhancing muscle power more afterwards, adjust hormone, reduction blood glucose and control
Blood pressure, control liver index and liver function health care etc., Ministry of Public Health's approval ginseng can be used for the production and processing of field of food within 2012, because
The research of the processing method of this ginseng has broader prospect.
Panaxan is one of main active constituent in ginseng, and at present, the separation and extraction technology of panaxan is compared
Common, people, which are mainly processed into dried food and nuts to the utilization of ginseng or extract its active ingredient, is used as medicine utilization, and processing of Panax ginseng is still
It is so that several original roughing type products such as sun-dried ginseng, red ginseng, dali ginseng circulate on the market.
But the conventional ginseng processing technology production cycle is long, production equipment is costly, affects and promotes processing of Panax ginseng technology
The process of innovation.
Invention content
The embodiment of the present invention is designed to provide a kind of method that ginseng pulp is prepared using cellulase, it is intended to solve to pass
Uniting, the processing of Panax ginseng technique productions period is long, production equipment is costly, affects asking for the process that promotes processing of Panax ginseng technological innovation
Topic.
The embodiment of the present invention is achieved in that a kind of method that ginseng pulp is prepared using cellulase hydrolysis, this utilizes fibre
The method that dimension element enzymolysis prepares ginseng pulp includes the following steps：
Step 1 takes ginseng to be cut into blocky or sheet, adds in pure water；
Step 2 is rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjusts pH value, is used
Autoclaving equipment boiling ginseng pulp；
Step 3 is adjusted ginseng pulp pH value before digesting, is heated in 50 DEG C of constant temperature water baths；Add the fiber of 800~2500 g tons
In plain enzyme to ginseng juice, digest 1 hour, then carry out centrifugal filtration processing；
Step 4 adjusts enzymolysis supernatant temperature, carries out destroy the enzyme treatment 15~30 minutes, obtains cellulosic sections hydrolysis
Ginseng pulp.
Further, in step 1, ginseng can join class Chinese herbal medicine and by east for northeasterner's ginseng, Korean ginseng, American Ginseng
Red ginseng, suncured white ginseng root, the sun-dried ginseng product that Radix Ginseng, Korean ginseng, American Ginseng ginseng class Chinese herbal medicine are prepared into.
Further, in step 1, it is 1: 8~1: 12 to add in pure water to solid-liquid ratio.
Further, in step 2, ginseng pulp is fully refined using colloid mill and adjusts pH value to 3.2~3.8, autoclaving
105~115 DEG C of temperature, digestion time 10~15 minutes.
Further, in step 3, ginseng pulp PH is adjusted to 6.5 before enzymolysis.
Further, in step 4, the temperature for digesting supernatant is 100 DEG C.
The method provided by the invention that ginseng pulp is prepared using cellulase hydrolysis, by ginseng after cellulase degradation,
From solid state toward liquefaction, macromolecular cellulose becomes small molecule glucose, and cellulase can destroy cell wall, passes through
After enzymatic treatment, the dissolution rate of cellular content can be improved, the functional components such as ginsenoside, glucide is made to be completely discharged to enzyme
It solves in liquid.It is many that the liquefaction ginseng pulp that the present invention obtains can be applied to food, drug, health products, cosmetics and wine brewing processing etc.
Aspect is widely used, simple production process, solves that the conventional ginseng processing technology production cycle is long, production equipment is costly
The effect of problem, the efficient active ingredient obtained in ginseng, effective protection ginseng ingredient, and promote processing of Panax ginseng skill
The process of art innovation.In addition, simple production process of the present invention, production cost is relatively low, convenient for industrialized production.
Description of the drawings
Fig. 1 is the flow chart of the method provided in an embodiment of the present invention that ginseng pulp is prepared using cellulase hydrolysis.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Fig. 1 shows the method flow provided by the invention that ginseng pulp is prepared using cellulase hydrolysis.For convenience of description,
Only the parts related to the present invention are shown.
The method that ginseng pulp is prepared using cellulase hydrolysis of the present invention, this prepares the side of ginseng pulp using cellulase hydrolysis
Method includes the following steps：
Step 1 takes ginseng to be cut into blocky or sheet, adds in pure water；
Step 2 is rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjusts pH value, is used
Autoclaving equipment boiling ginseng pulp；
Step 3 is adjusted ginseng pulp pH value before digesting, is heated in 50 DEG C of constant temperature water baths；Add the fiber of 800~2500 g tons
In plain enzyme to ginseng juice, digest 1 hour, then carry out centrifugal filtration processing；
Step 4 adjusts enzymolysis supernatant temperature, carries out destroy the enzyme treatment 15~30 minutes, obtains cellulosic sections hydrolysis
Ginseng pulp.
As a prioritization scheme of the embodiment of the present invention, in step 1, ginseng can be northeasterner's ginseng, Korean ginseng, west
American ginseng joins class Chinese herbal medicine and the red ginseng being prepared by northeast ginseng, Korean ginseng, American Ginseng ginseng class Chinese herbal medicine, suncured white ginseng root, sun-dried
Join product.
As a prioritization scheme of the embodiment of the present invention, in step 1, it is 1: 8~1 to add in pure water to solid-liquid ratio:
12。
As a prioritization scheme of the embodiment of the present invention, in step 2, ginseng pulp is fully refined using colloid mill and is adjusted
PH value is to 3.2~3.8,105~115 DEG C of autoclaving temperature, digestion time 10~15 minutes.
As a prioritization scheme of the embodiment of the present invention, in step 3, ginseng pulp PH is adjusted to 6.5 before enzymolysis.
As a prioritization scheme of the embodiment of the present invention, in step 4, the temperature for digesting supernatant is 100 DEG C.
Below in conjunction with the accompanying drawings and specific embodiment is further described the application principle of the present invention.
As shown in Figure 1, the method for preparing ginseng pulp using cellulase hydrolysis of the embodiment of the present invention includes the following steps：
S101：Fresh ginseng is taken to be cut into blocky or sheet, it is 1: 8~1: 12 to add in pure water to solid-liquid ratio；
S102：Rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjust pH value to 3.2~
3.8, use autoclaving equipment boiling ginseng pulp, 105~115 DEG C of boiling temperature, digestion time 10~15 minutes；
S103：Ginseng pulp PH is adjusted to 6.5 before enzymolysis, is heated in 50 DEG C of constant temperature water baths；Add 800~2500 g tons
In cellulase to ginseng juice, digest 1 hour, then carry out centrifugal filtration processing；
S104：Supernatant is digested under the conditions of 100 DEG C, carries out destroy the enzyme treatment 15~30 minutes, obtains cellulosic sections water
The ginseng pulp of solution.
The present invention the specific steps are：
Fresh ginseng is taken to be cut into blocky or sheet, ginseng can join class Chinese herbal medicine for northeasterner's ginseng, Korean ginseng, American Ginseng,
And red ginseng, suncured white ginseng root, the sun-dried ginseng product being prepared by northeast ginseng, Korean ginseng, American Ginseng ginseng class Chinese herbal medicine, it adds in pure
Water is to solid-liquid ratio for 1: 8~1: 12 (if fresh ginseng, solid-liquid ratio 1: 8~1: 10；If trepang, solid-liquid ratio 1: 10~1:
12) it, is rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjusts pH value to 3.2~3.8, is made
With autoclaving equipment boiling ginseng pulp, 105~115 DEG C of boiling temperature, digestion time 10~15 minutes.By ginseng pulp before enzymolysis
PH is adjusted to 6.5, is heated in 50 DEG C of constant temperature water baths；In the cellulase to ginseng pulp for adding 800~2500 g tons, enzymolysis 1 is small
When, centrifugal filtration processing is then carried out, enzymolysis supernatant carries out destroy the enzyme treatment 15~30 minutes under the conditions of 100 DEG C, obtains fine
The ginseng pulp of the plain partial hydrolysis of dimension.
The specific embodiment of the present invention：
Embodiment 1
1 kilogram of fresh long RADIX GINSENG ALBA in northeast is taken to be cut into block or piece, adds in 10 liters of pure water, is twisted with cooking machine or pulverizer
It is broken, ginseng pulp is then fully refined using colloid mill and adjusts pH value to 3.5, using autoclaving equipment boiling ginseng pulp, is steamed
110 DEG C of temperature is boiled, digestion time 10 minutes obtains ginseng pulp, takes the long RADIX GINSENG ALBA's slurry in northeast, is adjusted with certain density citric acid
PH value is heated to 50 DEG C of holding constant temperature to 6.5, with water-bath, adds cellulase by 2500 g ton of additive amount, hydrolyzes 1 hour,
Then centrifugal filtration processing is carried out, enzymolysis supernatant carries out destroy the enzyme treatment 15 minutes under the conditions of 100 DEG C, obtains cellulose portion
Divide 9800 milliliters of the ginseng pulp of hydrolysis；
Embodiment 2
300 grams of fresh American Ginsengs is taken to be cut into block or piece, add in 10 liters of pure water, are rubbed with cooking machine or pulverizer, then
Ginseng pulp is fully refined using colloid mill and adjusts pH value to 3.5, uses autoclaving equipment boiling ginseng pulp, boiling temperature
110 DEG C, digestion time 10 minutes obtains full constituent ginseng juice, American Ginseng is taken to starch, with certain density citric acid adjustment pH value extremely
6.5,50 DEG C of holding constant temperature are heated to water-bath, cellulase is added by 1500 g ton of additive amount, hydrolyzes 1 hour, Ran Houjin
Row centrifugal filtration is handled, and enzymolysis supernatant carries out destroy the enzyme treatment 15 minutes under the conditions of 100 DEG C, obtains cellulosic sections hydrolysis
9850 milliliters of ginseng pulp；
Embodiment 3
1 kilogram of fresh Korean ginseng is taken to be cut into block or piece, adds in 10 liters of pure water, is rubbed with cooking machine or pulverizer, then
Ginseng pulp is fully refined using colloid mill and adjusts pH value to 3.5, uses autoclaving equipment boiling ginseng pulp, boiling temperature
110 DEG C, digestion time 10 minutes obtains full constituent ginseng juice, takes Korean ginseng full constituent ginseng juice chaos PID, and use is certain density
Citric acid adjusts pH value to 6.5, is heated to 50 DEG C of holding constant temperature with water-bath, cellulase is added by 1800 g ton of additive amount,
Hydrolysis 1 hour, then carries out centrifugal filtration processing, and enzymolysis supernatant carries out destroy the enzyme treatment 15 minutes under the conditions of 100 DEG C, obtains
Obtain 9700 milliliters of the ginseng pulp of cellulosic sections hydrolysis.
The present invention is widely used, simple production process, solves conventional ginseng processing technology production cycle length, production equipment
The effect of the problem of costly, the efficient active ingredient obtained in ginseng, effective protection ginseng ingredient, and promote people
Participate in the process of work technological innovation.In addition, simple production process of the present invention, production cost is relatively low, convenient for industrialized production.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (1)
- A kind of 1. method that ginseng pulp is prepared using cellulase hydrolysis, which is characterized in that this prepares ginseng using cellulase hydrolysis The method of slurry includes the following steps：Step 1 takes ginseng to be cut into blocky or sheet, adds in pure water；Step 2 is rubbed with cooking machine or pulverizer, ginseng pulp is then fully refined using colloid mill and adjusts pH value, uses high pressure Digesting apparatus boiling ginseng pulp；Step 3, mediator's pH value of the ginseng pulp before enzymolysis are heated in 50 DEG C of constant temperature water baths；Add the cellulase of 800~2500 g tons Into ginseng juice, digest 1 hour, then carry out centrifugal filtration processing；Step 4 adjusts enzymolysis supernatant temperature, carries out destroy the enzyme treatment 15~30 minutes, obtains the ginseng of cellulosic sections hydrolysis Slurry；In step 1, ginseng for northeast ginseng, Korean ginseng, American Ginseng join class Chinese herbal medicine and by northeast ginseng, Korean ginseng, Red ginseng, suncured white ginseng root, the sun-dried ginseng product that American Ginseng ginseng class Chinese herbal medicine is prepared into；In step 1, it is 1: 8~1: 12 to add in pure water to solid-liquid ratio；In step 2, ginseng pulp is fully refined using colloid mill and adjusts pH value to 3.2~3.8, autoclaving temperature 105~ 115 DEG C, digestion time 10~15 minutes；In step 3, ginseng pulp pH is adjusted to 6.5 before enzymolysis；In step 4, the temperature for digesting supernatant is 100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310355912.6A CN103446208B (en) | 2013-08-11 | 2013-08-11 | A kind of method that ginseng pulp is prepared using cellulase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310355912.6A CN103446208B (en) | 2013-08-11 | 2013-08-11 | A kind of method that ginseng pulp is prepared using cellulase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103446208A CN103446208A (en) | 2013-12-18 |
| CN103446208B true CN103446208B (en) | 2018-06-19 |
Family
ID=49729322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310355912.6A Active CN103446208B (en) | 2013-08-11 | 2013-08-11 | A kind of method that ginseng pulp is prepared using cellulase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103446208B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104687016A (en) * | 2015-02-02 | 2015-06-10 | 吉林农业大学 | Method for preparing black fungus all-component protoplasm by utilizing cellulase |
| CN106309613B (en) * | 2015-07-01 | 2020-07-21 | 莱博药妆技术（上海）股份有限公司 | Chinese medicine with anti-inflammatory and anti-allergic effects and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325693A (en) * | 2000-05-29 | 2001-12-12 | 厦门中卫多糖研究所 | Process for preparing ginseng extract powder |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1186637A (en) * | 1998-01-14 | 1998-07-08 | 马辉 | Health genseng drink and its preparing method |
| JP4767686B2 (en) * | 2003-09-30 | 2011-09-07 | 湧永製薬株式会社 | Medicine for liver protection |
-
2013
- 2013-08-11 CN CN201310355912.6A patent/CN103446208B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325693A (en) * | 2000-05-29 | 2001-12-12 | 厦门中卫多糖研究所 | Process for preparing ginseng extract powder |
Non-Patent Citations (1)
| Title |
|---|
| 正交试验优选人参中人参皂苷Rg1的酶解工艺研究;赵啸虎等;《中草药》;20100831;第41卷(第8期);第1279-1282页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103446208A (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719880B (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN103540493B (en) | A kind of compound method of ganoderma lucidum tea wine | |
| CN103445159B (en) | A kind of enzymatic-process preparation method of full constituent ginseng juice | |
| CN105482957A (en) | Rhizoma polygonati extract, rhizoma polygonati wine thereof and rhizoma polygonati wine preparation method | |
| CN106036341A (en) | Method for increasing effective components in noni powder | |
| CN104774228A (en) | Method for preparing persimmon tannin from persimmon | |
| CN103504441B (en) | Food health electuary of a kind of improving water flood and preparation method thereof | |
| CN104788511A (en) | Method for preparing persimmon tannins with high micromolecular tannin content from persimmons | |
| CN104140471A (en) | Method for preparing yam high purity polysaccharide from yam food processing leftovers | |
| CN104031734A (en) | Method for preparing sesame oil by enzymolysis and supercritical fluid extraction | |
| CN103446208B (en) | A kind of method that ginseng pulp is prepared using cellulase | |
| CN107927513A (en) | A kind of preparation method of instant burdock solid beverage | |
| CN104263589A (en) | Manufacturing method of maca fermented wine | |
| CN103621929A (en) | Method for preparing sugar-free and smell-free pumpkin powder | |
| CN114568709A (en) | Extraction method for improving water-soluble dietary fiber content of square bamboo shoots | |
| CN103864956B (en) | A kind of method extracting pectin from eggplant fruit | |
| CN105942099A (en) | Red-date fermented beverage and production method thereof | |
| CN109170885A (en) | A method of extracting ginseng effective component | |
| CN103445157B (en) | Clarification method of ultrafiltration enzyme in preparation of ginseng juice | |
| CN104509921A (en) | Fructus momordicae beverage preparation method | |
| CN103947993B (en) | A kind of preparation method of nutrient condiments and goods | |
| CN103445160A (en) | Method for preparing ginseng pulp through enzymolysis of pectinase and ginseng pulp | |
| CN102823844A (en) | Kelp jam rich in dietary fiber | |
| CN103497873B (en) | A kind of active Gastrodia elata original plasm wine and preparation method thereof | |
| CN104189071A (en) | Preparation method of flavored motherwort paste |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Junmei Inventor after: Liu Fangming Inventor after: Li Zhuowei Inventor after: Wu Jie Inventor after: Fan Chunyan Inventor after: Hu Yaohui Inventor after: Zhang Jingchen Inventor after: Wang Yuhua Inventor after: Pu Chunhong Inventor after: Yu Hansong Inventor after: Dai Weichang Inventor after: Fang Xin Inventor after: Yu Xiaoming Inventor before: Hu Yaohui Inventor before: Liu Fangming Inventor before: Li Zhuowei Inventor before: Wu Jie Inventor before: Fan Chunyan Inventor before: Zhang Jingchen Inventor before: Wang Yuhua Inventor before: Liu Junmei Inventor before: Pu Chunhong Inventor before: Yu Hansong Inventor before: Dai Weichang Inventor before: Fang Xin Inventor before: Yu Xiaoming |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |