CN103445157B - Clarification method of ultrafiltration enzyme in preparation of ginseng juice - Google Patents

Clarification method of ultrafiltration enzyme in preparation of ginseng juice Download PDF

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CN103445157B
CN103445157B CN201310355889.0A CN201310355889A CN103445157B CN 103445157 B CN103445157 B CN 103445157B CN 201310355889 A CN201310355889 A CN 201310355889A CN 103445157 B CN103445157 B CN 103445157B
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ginseng
pulp
juice
enzyme
ultrafiltration
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CN103445157A (en
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胡耀辉
刘芳茗
王玉华
刘俊梅
朴春红
于寒松
代伟长
房鑫
张静晨
于晓明
李琢伟
吴杰
范春艳
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Jilin Agricultural University
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Jilin Agricultural University
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Abstract

The invention discloses a clarification method of ultrafiltration enzyme in the preparation of ginseng juice. The clarification method comprises the following steps: cutting clean ginseng into slices with the thickness of 5mm, adding purified water to a material-liquid ratio of 1:8-1:12, mincing by using a pulverizer, and then finely grinding ginseng pulp by using a colloid mill; adjusting pH value by adopting citric acid and lactic acid; carrying out high-temperature and high-pressure treatment; cooling, placing the ginseng pulp into an enzymolysis tank, adjusting pH value by using sodium bicarbonate, preserving heat and carrying out hydrolysis; and carrying out enzyme deactivation treatment at 100 DEG C, carrying out centrifugal treatment and filtering the ginseng pulp to obtain clarified ginseng juice. After the ginseng pulp prepared by the clarification method is treated by the ultrafiltration enzyme, macromolecular substances such as cellulose, pectin and protein are partially hydrolyzed to be in a micromolecular state, so that the ginseng juice is obviously clarified, the yield of the ginseng juice is increased, and the flavor of the ginseng juice is improved; meanwhile, the utilization rate of the ginseng can be effectively increased, the production process is easy, the operation is convenient, the cost is low, and the clarification method is suitable for large-scale industrial production.

Description

A kind of ultrafiltration enzyme is preparing the defecation method in ginseng juice
Technical field
The invention belongs to liquid ginseng product preparation field, particularly relate to a kind of ultrafiltration enzyme and preparing the defecation method in ginseng juice.
Background technology
Why very rare the good reputation that ginseng has since ancient times " kings of hundred grass " is, very famous and precious, main relevant with its medical value.Just think in medical book Shennong's Herbal very early, effect that ginseng has " tonifying five zang organs, peace spirit, determine soul, only palpitation with fear, except perverse trend, the happy intelligence development of improving eyesight ", " take to make light of one's life by commiting suicide for a long time and prolong life ".Li Shizhen (1518-1593 A.D.) is also very praised highly ginseng in Compendium of Material Medica, thinks that it " can control all chronic diseases marked by deficiency of vital energy of male woman ".For thousands of years, ginseng is all listed in " top grade ".According to the regulation of " People's Republic of China's the law of food safety " and " new resource food management method ", it is new resource food that the Ministry of Public Health in 2012 ratifies ginseng (artificial growth), Leaf-feeding insects is: root and rhizome, and ginseng changes food resource into from medicinal.At present, ginseng can be widely used in the fields such as food, medicine, cosmetics and wine brewing.
In the production of liquid ginseng product, clarification operation is the key determining its presentation quality, as dealt with improperly, not only affects product appearance, and directly has influence on quality and the stability of liquid ginseng product.Ultrafiltration enzyme is a kind of complex enzyme, and ginseng pulp not only can be made to clarify, and the compositions such as protein wherein, pectin, cellulose can also be decomposed further, realize the efficiency utilization of ginseng.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of ultrafiltration enzyme preparing the defecation method in ginseng juice, the method being intended to solve the liquid ginseng product of existing production lacks scientific and reasonable design, causes the quality of liquid ginseng product and the problem of poor stability of preparation.
The embodiment of the present invention is achieved in that a kind of ultrafiltration enzyme is preparing the defecation method in ginseng juice, and this ultrafiltration enzyme comprises the following steps preparing the defecation method in ginseng juice:
Step one, get the sheet that clean ginseng is cut into 5 mm of thickness, adding pure water to solid-liquid ratio is 1: 8 ~ 1: 12, rubs with pulverizer, then uses colloid mill fine grinding ginseng pulp;
Step 2, employing citric acid, lactic acid adjusted to ph;
Step 3, high temperature high pressure process;
Step 4, cooling, put into enzymatic vessel by ginseng pulp, regulates pH with sodium acid carbonate, insulation, hydrolysis;
Step 5,100 DEG C of ferment treatment that go out, carry out centrifugal treating and filter the ginseng juice that ginseng pulp obtains clarification.
Further, fresh ginseng when adding pure water if ginseng is cut into slices, solid-liquid ratio is 1: 8 ~ 1: 10; If trepang, solid-liquid ratio is 1: 10 ~ 1: 12.
Further, the pulverizer rubbing ginseng employing can adopt cooking machine to replace.
Further, can adopt in citric acid, lactic acid in step 2 during adjusted to ph any one, also can use simultaneously; Adjusted to ph to 3.2 ~ 3.8 in step 2.
Further, in step 3, the temperature of high temperature high pressure process is 105 ~ 115 DEG C, and the processing time is 10 ~ 15 minutes.
Further, be cooled to 50 ~ 60 DEG C in step 4, adopt sodium acid carbonate to regulate pH to 3.8 ~ 4.2 before enzymolysis, the addition adding ultrafiltration enzyme carries out according to 80.0 ~ 100.0 milliliters/ton, and holding temperature is 50 ~ 60 DEG C, and hydrolysis time is 2 hours.
Further, the time of the ferment treatment that goes out in step 5 is 15 ~ 30 minutes, and the rotating speed of the centrifuge of centrifugal filtration ginseng pulp is 10000 revs/min, and the centrifugal time is 10 minutes.
Further, described enzyme preparation is ultrafiltration enzyme.
Further, northeast ginseng, Korean ginseng and American Ginseng and the red ginseng be prepared into by these ginsengs, suncured white ginseng root, sun-dried ginseng product all can adopt ultrafiltration enzyme to clarify the liquid ginseng product of preparation.
The invention provides a kind of ultrafiltration enzyme and prepare the defecation method in ginseng juice, ginseng pulp prepared by the method is after ultrafiltration enzyme process, the macromolecular substances partial hydrolysis such as cellulose, pectin, protein become Small molecular state, ginseng juice is obviously clarified, improves the productive rate of ginseng juice and improve the local flavor of ginseng juice.The present invention can effectively improve ginseng utilization rate, and production technology is simple, and easy to operate, cost is low, is suitable for large-scale industrial production.
Accompanying drawing explanation
Fig. 1 is that the ultrafiltration enzyme that the embodiment of the present invention provides is preparing the defecation method flow chart in ginseng juice.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Fig. 1 shows ultrafiltration enzyme provided by the invention in the flow process preparing the defecation method in ginseng juice.For convenience of explanation, illustrate only part related to the present invention.
The ultrafiltration enzyme that the embodiment of the present invention provides comprises the following steps preparing the defecation method in ginseng juice:
Step one, get the sheet that clean ginseng is cut into 5 mm of thickness, adding pure water to solid-liquid ratio is 1: 8 ~ 1: 12, rubs with pulverizer, then uses colloid mill fine grinding ginseng pulp;
Step 2, employing citric acid, lactic acid adjusted to ph;
Step 3, high temperature high pressure process;
Step 4, cooling, put into enzymatic vessel by ginseng pulp, regulates pH with sodium acid carbonate, insulation, hydrolysis;
Step 5,100 DEG C of ferment treatment that go out, carry out centrifugal treating and filter the ginseng juice that ginseng pulp obtains clarification.
As a prioritization scheme of the embodiment of the present invention, fresh ginseng when adding pure water if ginseng is cut into slices, solid-liquid ratio is 1: 8 ~ 1: 10; If trepang, solid-liquid ratio is 1: 10 ~ 1: 12.
As a prioritization scheme of the embodiment of the present invention, the pulverizer rubbing ginseng employing can adopt cooking machine to replace.
As a prioritization scheme of the embodiment of the present invention, can adopt in citric acid, lactic acid in step 2 during adjusted to ph any one, also can use simultaneously; Adjusted to ph to 3.2 ~ 3.8 in step 2.
As a prioritization scheme of the embodiment of the present invention, in step 3, the temperature of high temperature high pressure process is 105 ~ 115 DEG C, and the processing time is 10 ~ 15 minutes.
As a prioritization scheme of the embodiment of the present invention, be cooled to 50 ~ 60 DEG C in step 4, adopt sodium acid carbonate to regulate pH to 3.8 ~ 4.2 before enzymolysis, the addition adding ultrafiltration enzyme carries out according to 80.0 ~ 100.0 milliliters/ton, holding temperature is 50 ~ 60 DEG C, and hydrolysis time is 2 hours.
As a prioritization scheme of the embodiment of the present invention, the time of the ferment treatment that goes out in step 5 is 15 ~ 30 minutes, and the rotating speed of the centrifuge of centrifugal filtration ginseng pulp is 10000 revs/min, and the centrifugal time is 10 minutes.
As a prioritization scheme of the embodiment of the present invention, described enzyme preparation is ultrafiltration enzyme.
As a prioritization scheme of the embodiment of the present invention, northeast ginseng, Korean ginseng and American Ginseng and the red ginseng be prepared into by these ginsengs, suncured white ginseng root, sun-dried ginseng product all can adopt ultrafiltration enzyme to clarify the liquid ginseng product of preparation.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The ultrafiltration enzyme that the embodiment of the present invention provides comprises the following steps preparing the defecation method in ginseng juice:
S101: get the sheet that clean ginseng is cut into 5 mm of thickness, adding pure water to solid-liquid ratio is 1: 8 ~ 1: 12, rubs with pulverizer, then uses colloid mill fine grinding ginseng pulp;
S102: adopt citric acid, lactic acid adjusted to ph;
S103: high temperature high pressure process;
S104: cooling, puts into enzymatic vessel by ginseng pulp, regulates pH with sodium acid carbonate, insulation, hydrolysis;
The S105:100 DEG C of ferment treatment that goes out, carries out centrifugal treating and filters the ginseng juice that ginseng pulp obtains clarification.
Below in conjunction with specific embodiment, the detailed description of the invention that ultrafiltration enzyme is preparing the defecation method in ginseng juice is described in detail.
Embodiment 1
Get clean fresh northeast ginseng 100 grams, be cut into the sheet of 5 millimeter thickness, add pure water 1000 milliliters, use the abundant comminuting matter of food processor to even pulpous state, by citric acid adjusted to ph to 3.5, use high temperature and high pressure method process ginseng pulp, temperature 110 DEG C, 10 minutes time.Be cooled to 50 ~ 60 DEG C, ginseng pulp is put into enzymatic vessel, regulate ginseng pulp pH to 4.0 with sodium bicarbonate solution, heating water bath, add ultrafiltration enzyme 0.105 milliliter when 55 DEG C, thermostatic effect 2 hours.After enzymolysis completes, ginseng pulp is under 100 DEG C of conditions, and carry out going out ferment treatment 15 minutes, then carries out centrifugal filtration process (rotating speed of centrifuge is 10000 revs/min, and the centrifugal time is 10 minutes), final 930 milliliters, the ginseng juice obtaining clarification.
Embodiment 2
Get clean fresh Korean ginseng 100 grams, be cut into the sheet of 5 millimeter thickness, add pure water 1000 milliliters, use the abundant comminuting matter of food processor to even pulpous state, by citric acid adjusted to ph to 3.5, use high temperature and high pressure method process ginseng pulp, temperature 110 DEG C, 15 minutes time.Be cooled to 50 ~ 60 DEG C, ginseng pulp is put into enzymatic vessel, regulate ginseng pulp pH to 4.0 with sodium bicarbonate solution, heating water bath, add ultrafiltration enzyme 0.1 milliliter when 55 DEG C, thermostatic effect 2 hours.After enzymolysis completes, ginseng pulp is under 100 DEG C of conditions, and carry out going out ferment treatment 15 minutes, then carries out centrifugal filtration process (rotating speed of centrifuge is 10000 revs/min, and the centrifugal time is 10 minutes), final 915 milliliters, the ginseng juice obtaining clarification.
Embodiment 3
Get clean fresh American Ginseng 100 grams, be cut into the sheet of 5 millimeter thickness, add pure water 1000 milliliters, use the abundant comminuting matter of food processor to even pulpous state, by citric acid adjusted to ph to 3.5, use high temperature and high pressure method process ginseng pulp, temperature 110 DEG C, 10 minutes time.Be cooled to 50 ~ 60 DEG C, ginseng pulp is put into enzymatic vessel, regulate ginseng pulp pH to 4.0 with sodium bicarbonate solution, heating water bath, add ultrafiltration enzyme 0.095 milliliter when 55 DEG C, thermostatic effect 2 hours.After enzymolysis completes, ginseng pulp is under 100 DEG C of conditions, and carry out going out ferment treatment 15 minutes, then carries out centrifugal filtration process (rotating speed of centrifuge is 10000 revs/min, and the centrifugal time is 10 minutes), final 908 milliliters, the ginseng juice obtaining clarification.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (1)

1. ultrafiltration enzyme is preparing the defecation method in ginseng juice, it is characterized in that, this ultrafiltration enzyme comprises the following steps preparing the defecation method in ginseng juice:
Step one, get clean ginseng and be cut into sheet, adding pure water to solid-liquid ratio is 1: 8 ~ 1: 12, rubs with pulverizer or cooking machine, then uses colloid mill fine grinding ginseng pulp;
Step 2, employing citric acid and/or lactic acid adjusted to ph;
Step 3, high temperature high pressure process;
Step 4, cooling, put into enzymatic vessel by ginseng pulp, regulates pH with sodium acid carbonate, insulation, hydrolysis;
Step 5,100 DEG C of ferment treatment that go out, carry out centrifugal treating and filter the ginseng juice that ginseng pulp obtains clarification;
Fresh ginseng when adding pure water if ginseng is cut into slices, solid-liquid ratio is 1: 8 ~ 1: 10; If trepang, solid-liquid ratio is 1: 10 ~ 1: 12;
Adjusted to ph to 3.2 ~ 3.8 in step 2;
In step 3, the temperature of high temperature high pressure process is 105 ~ 115 DEG C, and the processing time is 10 ~ 15 minutes;
Be cooled to 50 ~ 60 DEG C in step 4, adopt sodium acid carbonate to regulate pH to 3.8 ~ 4.2 before enzymolysis, the addition adding ultrafiltration enzyme carries out according to 80.0 ~ 100.0 milliliters/ton, and holding temperature is 50 ~ 60 DEG C, and hydrolysis time is 2 hours;
Go out in step 5 time of ferment treatment is 15 ~ 30 minutes, and the rotating speed of the centrifuge of centrifugal filtration ginseng pulp is 10000 revs/min, and the centrifugal time is 10 minutes;
Red ginseng, suncured white ginseng root, sun-dried ginseng product that described ginseng is selected from northeast ginseng, Korean ginseng and American Ginseng and is prepared into by these ginsengs.
CN201310355889.0A 2013-08-11 2013-08-11 Clarification method of ultrafiltration enzyme in preparation of ginseng juice Active CN103445157B (en)

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CN104721245A (en) * 2015-03-28 2015-06-24 珲春绿源参业生物科技有限公司 Method for making ginseng extraction component by multi-enzyme enzymolysis
CN109757639A (en) * 2019-03-20 2019-05-17 战铁楠 A kind of ginseng ternary peptide beverage and preparation method thereof
CN109757641A (en) * 2019-03-20 2019-05-17 战铁楠 A kind of ginseng small-molecular peptides beverage and preparation method thereof

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CN1186637A (en) * 1998-01-14 1998-07-08 马辉 Health genseng drink and its preparing method
CN1325693A (en) * 2000-05-29 2001-12-12 厦门中卫多糖研究所 Process for preparing ginseng extract powder
KR100496418B1 (en) * 2002-09-11 2005-06-28 한국파낙스제조(주) Manufacturing method of black rootlets of ginseng
CN100362947C (en) * 2003-04-04 2008-01-23 梅河口市人参有限公司 Ginseng American ginseng beverage and its production process
CN101278954A (en) * 2007-04-04 2008-10-08 赵方林 Ginseng or American ginseng product with increased content of ginseng saponin Rg3 and method of preparing the same
CN102600222B (en) * 2012-04-13 2013-08-07 吉林农业大学 Method for increasing content of rare saponin in ginseng raw material by explosion of steam
CN102987482A (en) * 2012-11-29 2013-03-27 新疆天海绿洲农业科技股份有限公司 Concentrated red date juice and preparation method thereof

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