CN104687016A - Method for preparing black fungus all-component protoplasm by utilizing cellulase - Google Patents
Method for preparing black fungus all-component protoplasm by utilizing cellulase Download PDFInfo
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- CN104687016A CN104687016A CN201510069200.7A CN201510069200A CN104687016A CN 104687016 A CN104687016 A CN 104687016A CN 201510069200 A CN201510069200 A CN 201510069200A CN 104687016 A CN104687016 A CN 104687016A
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Abstract
The invention discloses a method for preparing black fungus all-component protoplasm by utilizing cellulase. The method comprises the following steps: cleaning black fungus, removing photosensitive substances on the surface of the black fungus, drying and adding purified water; and stewing in a high-pressure steaming device, cooling the stewed black fungus, and finely milling fully, standing, and defoaming so as to obtain black fungus stoste; carrying out ultrasonic treatment on the obtained black fungus stoste, and adjusting the pH value; putting the black fungus stoste after adjusting the pH value into a constant-temperature water bath pot, heating, adding cellulase, carrying out water bath at constant temperature for 1 hour, and carrying out centrifugal filtration treatment; regulating the temperature and the pH value of the enzymatic supernate, and carrying out enzyme deactivation treatment. After the black fungus is subjected to enzymolysis by cellulase, the dissolution rate of the cellular content is improved, so that active substances such as polysaccharide are completely released into enzymatic hydrolysate from cells, the problems of complex operation, long operation time, active structure damage and low yield of the traditional black fungus processing technology are solved, effective ingredients in the black fungus are obtained simply and efficiently, and the functional components are protected.
Description
Technical field
The invention belongs to food production field, particularly relate to a kind of method utilizing cellulase to prepare black fungus full constituent magma.
Background technology
Black fungus is mainly distributed in area, north temperate zone, and China is one of country that out and out auricularia auriculajudae resource is the abundantest in the world, and be also auricularia auriculajudae production and consumption big country, the whole world has about 15 kinds, is almost all distributed in China.Black fungus is traditional edible and medical fungi that our people likes, its nutritive value is very high, and containing the protein being equivalent to meat, human body is higher to its absorptivity, and this is general rice, face, greengrocery food can not be by comparison.Black fungus can reduce the blood clotting of people, prevents heart coronary artery disease.Black fungus colloid has great absorption affinity, and energy gut purge moistening lung is the important health food of weaving and coal miner.Black fungus contains polysaccharose substance, has inhibitory action to the cancer cell of animal.
Auricularia polysaccharide is the important active substances of auricularia auriculajudae, its biologically active more and more comes into one's own, at present, to the separation and Extraction of Auricularia polysaccharide and pharmacological research more common, simple processing is the principal mode of current Edible mushroom processing, edible mushroom makes dry product outlet or domestic by simple oven dry, and black fungus converted products is little, only has packed, box-packed black fungus, the block black fungus of compression and few fresh-keeping black edible fungus.
Black fungus have lost a large amount of nutrition and delicate flavour material after making dry product, loses original local flavor.The welcome of the extremely domestic and international consumer of auricularia auriculajudae deep-processed food, but the deep processing of auricularia auriculajudae product is also in the starting stage at present, with low content of technology, added value of product is low, only has and carries out deep processing to black fungus, and it just can be made really to play a role, just can open up markets, could rise in value, preserve value.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of method utilizing cellulase to prepare black fungus full constituent magma, is intended to solve traditional auricularia auriculajudae processing technology trivial operations, and the operating time is long, destroys active structure, the problem that yield is low.
The present invention is achieved in that a kind of method utilizing cellulase to prepare black fungus full constituent magma comprises the following steps:
Step one, get black fungus cleaning, remove surperficial photoactive substance, dry at 90-110 DEG C, after oven dry, add pure water;
Step 2, put into autoclaving equipment boiling 15-20 minute at 100-110 DEG C, cooling is fully refined with colloid mill after being placed on and being fully crushed to even pulpous state in cooking machine, leaves standstill the foam that disappears, obtains black fungus stoste;
Black fungus stoste step 2 obtained before step 3, enzymolysis carries out ultrasonic process 25-35 minute, adjust ph at 55-65 DEG C of temperature;
Step 4, the black fungus stoste regulating pH value is placed in thermostat water bath heats, add cellulase, water bath with thermostatic control 1 hour, carries out centrifugal filtration process;
Step 5, adjustment enzymolysis supernatant temperature and adjusted to ph, carry out the ferment treatment 15-30 minute that goes out, and obtains the auricularia auriculajudae magma of cellulosic sections hydrolysis.
Further, in step one, the amount adding pure water is that every 1g black fungus adds 80ml-120ml.
Further, the adjust ph described in step 3, uses one or both adjusted to ph to 6.0 in citric acid or lactic acid.
Further, in step 4, when black fungus stoste temperature reaches 50 DEG C, the addition of 1%-1.5% adds cellulase by volume.
Further, in step 5, regulate enzymolysis supernatant temperature to 105 DEG C, adjusted to ph is to 3.8-4.0.
The method utilizing cellulase hydrolysis to prepare auricularia auriculajudae magma of the present invention, by auricularia auriculajudae after cellulase degradation, solid auricularia auriculajudae is liquefied, large molecules fibrin becomes small molecule glucose, auricularia auriculajudae cell is thick, wall thickness, and cellulase can destroy cell membrane, the dissolution rate of cellular content can be improved, polysaccharide isoreactivity material is discharged into completely in enzymolysis liquid from cell; The liquefaction auricularia auriculajudae magma that the present invention obtains can be used for the aspects such as food, medicine, health products; be widely used; processing technology is simple, and solve traditional auricularia auriculajudae processing technology trivial operations, the operating time is long; destroy active structure; the problem that yield is low, obtains the active ingredient in auricularia auriculajudae simply efficiently, and protects functional component; advance the process of auricularia auriculajudae process technology innovation, for the comprehensive exploitation of auricularia auriculajudae utilization and improve its economic worth there is practical significance.
Accompanying drawing explanation
Fig. 1 is that the cellulase hydrolysis that utilizes that the embodiment of the present invention provides prepares the method flow diagram of auricularia auriculajudae magma.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, this present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain this present invention, be not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
Fig. 1 shows the method flow utilizing cellulase hydrolysis to prepare auricularia auriculajudae magma of the present invention, and as shown in the figure, the present invention is achieved in that a kind of method utilizing cellulase to prepare black fungus full constituent magma comprises the following steps:
S101, get black fungus cleaning, remove surperficial photoactive substance, dry at 90-110 DEG C, after oven dry, add pure water;
S102, put into autoclaving equipment boiling 15-20 minute at 100-110 DEG C, cooling is fully refined with colloid mill after being placed on and being fully crushed to even pulpous state in cooking machine, leaves standstill the foam that disappears, obtains black fungus stoste;
Black fungus stoste step 2 obtained before S103, enzymolysis carries out ultrasonic process 25-35 minute, adjust ph at 55-65 DEG C of temperature;
S104, the black fungus stoste regulating pH value is placed in thermostat water bath heats, add cellulase, water bath with thermostatic control 1 hour, carries out centrifugal filtration process;
S105, adjustment enzymolysis supernatant temperature and adjusted to ph, carry out the ferment treatment 15-30 minute that goes out, and obtains the auricularia auriculajudae magma of cellulosic sections hydrolysis.
Further, in step S101, the amount adding pure water is that every 1g black fungus adds 80ml-120ml.
Further, the adjust ph described in step S103, uses one or both adjusted to ph to 6.0 in citric acid or lactic acid.
Further, in step S104, when black fungus stoste temperature reaches 50 DEG C, the addition of 1%-1.5% adds cellulase by volume.
Further, in step S105, regulate enzymolysis supernatant temperature to 105 DEG C, adjusted to ph is to 3.8-4.0.
Embodiment 1
Get appropriate Yan Bianqiuer to clean, to remove dust and the photoactive substance on surface, 100 DEG C of oven dry.After oven dry, get 20 Ke Yanbianqiuer, add 2000 milliliters of pure water mixing, put into high-temp steam sterilizing pot in 105 DEG C, boiling 20 minutes.After being fully crushed to even pulpous state in cooking machine, refine 20 minutes with colloid mill after cooling, leave standstill the foam that disappears a night, obtain black fungus stoste.Ultrasonic process is (ultrasonic temperature is 60 DEG C) after 30 minutes, uses citric acid adjusted to ph to 6.0; Black fungus stoste is put into enzymatic vessel, adds cellulase 0.1, be incubated 50 DEG C, be hydrolyzed 2 hours.Adjusted to ph to 3.8, under 100 DEG C of conditions, go out ferment treatment 20 minutes, and (it is 10000 revs/min that centrifuge obtains rotating speed then to carry out centrifugal filtration process, the centrifugal time is 10 minutes), finally obtain the black fungus full constituent magma 1930 milliliters clarified.
Embodiment 2
Get appropriate Changbai Mountain black fungus to clean, to remove dust and the photoactive substance on surface, 100 DEG C of oven dry.After oven dry, get 20 grams of Changbai Mountain black fungus, add 2000 milliliters of pure water mixing, put into high-temp steam sterilizing pot in 105 DEG C, boiling 20 minutes.After being fully crushed to even pulpous state in cooking machine, refine 20 minutes with colloid mill after cooling, leave standstill the foam that disappears a night, obtain black fungus stoste.Ultrasonic process is (ultrasonic temperature is 60 DEG C) after 30 minutes, uses citric acid adjusted to ph to 6.0; Black fungus stoste is put into enzymatic vessel, adds cellulase 0.12 milliliter, be incubated 50 DEG C, be hydrolyzed 1 hour.Adjusted to ph to 4.0, under 100 DEG C of conditions, go out ferment treatment 20 minutes, and (it is 10000 revs/min that centrifuge obtains rotating speed then to carry out centrifugal filtration process, the centrifugal time is 10 minutes), finally obtain the black fungus full constituent magma 1920 milliliters clarified.
Embodiment 3
Get Shulan, appropriate Changbai Mountain black fungus to clean, to remove dust and the photoactive substance on surface, 100 DEG C of oven dry.After oven dry, get 10 grams of Shulan black fungus, add 1000 milliliters of pure water mixing, put into high-temp steam sterilizing pot in 105 DEG C, boiling 20 minutes.After being fully crushed to even pulpous state in cooking machine, refine 20 minutes with colloid mill after cooling, leave standstill the foam that disappears a night, obtain black fungus stoste.Ultrasonic process is (ultrasonic temperature is 60 DEG C) after 30 minutes, uses citric acid adjusted to ph to 6.0; Black fungus stoste is put into enzymatic vessel, adds cellulase 0.08ml, be incubated 50 DEG C, be hydrolyzed 1 hour.Adjusted to ph to 3.8, under 100 DEG C of conditions, go out ferment treatment 20 minutes, then carries out centrifugal filtration process (it is 10000 revs/min that centrifuge obtains rotating speed, and the centrifugal time is 10 minutes), finally obtains the black fungus full constituent magma 950 milliliters clarified.
The method utilizing cellulase hydrolysis to prepare auricularia auriculajudae magma of the present invention, by auricularia auriculajudae after cellulase degradation, solid auricularia auriculajudae is liquefied, large molecules fibrin becomes small molecule glucose, auricularia auriculajudae cell is thick, wall thickness, and cellulase can destroy cell membrane, the dissolution rate of cellular content can be improved, polysaccharide isoreactivity material is discharged into completely in enzymolysis liquid from cell; The liquefaction auricularia auriculajudae magma that the present invention obtains can be used for the aspects such as food, medicine, health products; be widely used; processing technology is simple, and solve traditional auricularia auriculajudae processing technology trivial operations, the operating time is long; destroy active structure; the problem that yield is low, obtains the active ingredient in auricularia auriculajudae simply efficiently, and protects functional component; advance the process of auricularia auriculajudae process technology innovation, for the comprehensive exploitation of auricularia auriculajudae utilization and improve its economic worth there is practical significance.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. utilize cellulase to prepare a method for black fungus full constituent magma, it is characterized in that, this method utilizing cellulase to prepare black fungus full constituent magma comprises the following steps:
Step one, get black fungus cleaning, remove surperficial photoactive substance, dry at 90-110 DEG C, after oven dry, add pure water;
Step 2, put into autoclaving equipment boiling 15-20 minute at 100-110 DEG C, cooling is fully refined with colloid mill after being placed in cooking machine and being crushed to pulpous state, leaves standstill the foam that disappears, obtains black fungus stoste;
Black fungus stoste step 2 obtained before step 3, enzymolysis carries out ultrasonic process 25-35 minute, adjust ph at 55-65 DEG C of temperature;
Step 4, the black fungus stoste regulating pH value is placed in thermostat water bath heats, add cellulase, water bath with thermostatic control 1 hour, carries out centrifugal filtration process;
Step 5, adjustment enzymolysis supernatant temperature and adjusted to ph, carry out the ferment treatment 15-30 minute that goes out, and obtains the auricularia auriculajudae magma of cellulosic sections hydrolysis.
2. utilize cellulase to prepare the method for black fungus full constituent magma as claimed in claim 1, it is characterized in that, in step one, the amount adding pure water is that every 1g black fungus adds 80ml-120ml.
3. utilize cellulase to prepare the method for black fungus full constituent magma as claimed in claim 1, it is characterized in that, the adjust ph described in step 3, use one or both adjusted to ph to 6.0 in citric acid or lactic acid.
4. utilize cellulase to prepare the method for black fungus full constituent magma as claimed in claim 1, it is characterized in that, in step 4, when black fungus stoste temperature reaches 50 DEG C, the addition of 1%-1.5% adds cellulase by volume.
5. utilize cellulase to prepare the method for black fungus full constituent magma as claimed in claim 1, it is characterized in that, in step 5, regulate enzymolysis supernatant temperature to 105 DEG C, adjusted to ph is to 3.8-4.0.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109549959A (en) * | 2018-11-29 | 2019-04-02 | 福建农林大学 | A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic |
| CN111557434A (en) * | 2020-06-19 | 2020-08-21 | 陕西三八妇乐特医食品有限公司 | Method for preparing predigested black fungus nutritional product |
| CN112314822A (en) * | 2020-11-06 | 2021-02-05 | 中国海洋大学 | Black fungus raw juice and preparation method thereof |
| CN112401224A (en) * | 2020-09-08 | 2021-02-26 | 山东省农业科学院农产品研究所 | Edible fungus enzymatic hydrolysate and preparation method thereof |
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| CN103446208A (en) * | 2013-08-11 | 2013-12-18 | 吉林农业大学 | Method for preparing ginseng pulp by using cellulase |
| CN103445159A (en) * | 2013-08-11 | 2013-12-18 | 吉林农业大学 | Enzymatic preparation method of all-component ginseng juice |
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- 2015-02-02 CN CN201510069200.7A patent/CN104687016A/en active Pending
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| KR100545835B1 (en) * | 2002-12-05 | 2006-01-26 | 주식회사 엠디바이오알파 | Process of soy sauce production using waste water of the beancurd |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109549959A (en) * | 2018-11-29 | 2019-04-02 | 福建农林大学 | A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic |
| CN109549959B (en) * | 2018-11-29 | 2021-07-23 | 福建农林大学 | Method for extracting black fungus melanin by using cellulase-ultrasonic wave synergism |
| CN111557434A (en) * | 2020-06-19 | 2020-08-21 | 陕西三八妇乐特医食品有限公司 | Method for preparing predigested black fungus nutritional product |
| CN112401224A (en) * | 2020-09-08 | 2021-02-26 | 山东省农业科学院农产品研究所 | Edible fungus enzymatic hydrolysate and preparation method thereof |
| CN112401224B (en) * | 2020-09-08 | 2022-11-15 | 山东省农业科学院农产品研究所 | Edible fungus enzymatic hydrolysate and preparation method thereof |
| CN112314822A (en) * | 2020-11-06 | 2021-02-05 | 中国海洋大学 | Black fungus raw juice and preparation method thereof |
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