CN105367678A - Lycium barbarum polysaccharide extraction method - Google Patents
Lycium barbarum polysaccharide extraction method Download PDFInfo
- Publication number
- CN105367678A CN105367678A CN201510876198.4A CN201510876198A CN105367678A CN 105367678 A CN105367678 A CN 105367678A CN 201510876198 A CN201510876198 A CN 201510876198A CN 105367678 A CN105367678 A CN 105367678A
- Authority
- CN
- China
- Prior art keywords
- lycium barbarum
- barbarum polysaccharide
- polysaccharide
- lbp
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000008518 lycium barbarum polysaccharide Substances 0.000 title claims abstract description 35
- 238000000605 extraction Methods 0.000 title abstract description 10
- 244000241838 Lycium barbarum Species 0.000 claims abstract description 18
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 8
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 8
- 239000005017 polysaccharide Substances 0.000 claims abstract description 8
- 238000003809 water extraction Methods 0.000 claims abstract description 8
- 150000004676 glycans Chemical class 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 6
- 229940088598 enzyme Drugs 0.000 claims description 10
- 235000015468 Lycium chinense Nutrition 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000010792 warming Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims description 2
- 229940106157 cellulase Drugs 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000470 constituent Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 208000004930 Fatty Liver Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010047513 Vision blurred Diseases 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- -1 semi-lactosi Chemical compound 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a lycium barbarum polysaccharide (LBP) extraction method, and belongs to the technical field of food processing. The LBP is water-soluble polysaccharide extracted from the lycium barbarum. The method disclosed by the present invention combing traditional water extraction and modern enzyme engineering extraction of the LBP can effectively improve the extraction rate of LBP and can retain active ingredients of the LBP as many as possible. The content of the present invention comprises: 1. manufacturing of lycium barbarum dried particles; 2. enzyme extraction of the LBP; and 3. water extraction of the LBP.
Description
Technical field
The present invention relates to a kind of extraction working method of lycium barbarum polysaccharide, belong to food processing technology field.
Background technology
Matrimony vine is China's tradition rare traditional Chinese medicine, have " red treasured " laudatory title, is rich in vegetable polysaccharides, protein, vitamins and other nutritious components.
Lycium barbarum polysaccharide is a kind of water-soluble polysaccharide extracting from matrimony vine and obtain.Clearly this polysaccharide system protein-polysaccharide, be made up of pectinose, glucose, semi-lactosi, seminose, wood sugar, rhamnosyl 6 kinds of monosaccharide components. show after deliberation, the main active ingredient that lycium barbarum polysaccharide is wolfberry fruit immunity moderation, delay senility, the symptoms such as the elderly's fatiguability, poor appetite and blurred vision can be improved, and there is reducing blood-fat, anti-fatty liveranti-fatty liver, the anti-ageing effect of waiting for a long time.
Lycium barbarum polysaccharide can be used as multi-medicament, raw materials of food processing, and lycium barbarum polysaccharide extracts and is more common in alcohol extracting and ultrasonic extraction, but these methods easily destroy the effective constituent of lycium barbarum polysaccharide in various degree.Enzyme engineering technology is in recent years for a biotechnology of active ingredient of natural plant extraction.Select appropriate enzyme, more leniently plant tissue can be decomposed, accelerate the release of effective constituent, thus improve its extraction yield.
The present invention extracts lycium barbarum polysaccharide in conjunction with traditional water extraction and modern enzyme engineering, effectively can improve the extraction yield of lycium barbarum polysaccharide, and retain the effective constituent of lycium barbarum polysaccharide as far as possible.
Summary of the invention
Tea lycium barbarum polysaccharide can be used as multi-medicament, raw materials of food processing, lycium barbarum polysaccharide extracts and is more common in alcohol extracting and ultrasonic extraction, the present invention extracts lycium barbarum polysaccharide in conjunction with traditional water extraction and modern enzyme engineering, effectively can improve the extraction yield of lycium barbarum polysaccharide, and retain the effective constituent of lycium barbarum polysaccharide as far as possible.Summary of the invention comprises: 1. the making of matrimony vine dried particles; 2. enzyme carries lycium barbarum polysaccharide; 3. water extraction lycium barbarum polysaccharide.
Embodiment
1. matrimony vine selection and preliminary working: 1) get lycium barbarum, screening cleaning; 2) dry, cleaned matrimony vine is put into fixation machine stir-fry, dry, between dry temperature 150-200 DEG C, time 3-5 minute, is dried to moisture less than 10%; 3) pulverize, dried wolfberry fruit powder is broken into 10 order particles.
2. enzyme carries lycium barbarum polysaccharide: 1) wolfberry fruit powder and water are mixed by 1:15; 2) 40 DEG C are warming up to; 3) cellulase of 0.01% concentration is added, enzymolysis 30 minutes; 4) 85 DEG C of enzymes that go out are warming up to.
3. water extraction lycium barbarum polysaccharide: 1) by enzymolysis tea polysaccharide lysate obtained above, to add water mixing according to 1:2; 2) mixed solution is heated to 60 DEG C 80 minutes; 3) filter, 150 mesh filter screens filter, and are divided into filtered liquid and filter residue; 4) filtered liquid is centrifugal, dry, can lycium barbarum polysaccharide be obtained.
Claims (4)
1. an extracting method for lycium barbarum polysaccharide, is characterized in that: extract and a kind of water-soluble polysaccharide of obtaining from matrimony vine; Summary of the invention comprises: summary of the invention comprises: 1. the making of matrimony vine dried particles; 2. enzyme carries lycium barbarum polysaccharide; 3. water extraction lycium barbarum polysaccharide.
2. the method according to claims 1, is characterized in that: the making of described matrimony vine dried particles refers to: 1) get lycium barbarum, screening cleaning; 2) dry, cleaned matrimony vine is put into fixation machine stir-fry, dry, between dry temperature 150-200 DEG C, time 3-5 minute, is dried to moisture less than 10%; 3) pulverize, dried wolfberry fruit powder is broken into 10 order particles.
3. the method according to claims 1, is characterized in that: described enzyme is carried lycium barbarum polysaccharide and referred to: 1) wolfberry fruit powder and water are mixed by 1:15; 2) 40 DEG C are warming up to; 3) cellulase of 0.01% concentration is added, enzymolysis 30 minutes; 4) 85 DEG C of enzymes that go out are warming up to.
4. the method according to claims 1, is characterized in that: described water extraction lycium barbarum polysaccharide refers to: 1) by enzymolysis tea polysaccharide lysate obtained above, to add water mixing according to 1:2; 2) mixed solution is heated to 60 DEG C 80 minutes; 3) filter, 150 mesh filter screens filter, and are divided into filtered liquid and filter residue; 4) filtered liquid is centrifugal, dry, can lycium barbarum polysaccharide be obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510876198.4A CN105367678A (en) | 2015-12-03 | 2015-12-03 | Lycium barbarum polysaccharide extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510876198.4A CN105367678A (en) | 2015-12-03 | 2015-12-03 | Lycium barbarum polysaccharide extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105367678A true CN105367678A (en) | 2016-03-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510876198.4A Pending CN105367678A (en) | 2015-12-03 | 2015-12-03 | Lycium barbarum polysaccharide extraction method |
Country Status (1)
| Country | Link |
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| CN (1) | CN105367678A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329399A (en) * | 2018-05-15 | 2018-07-27 | 重庆市洲仨科技发展有限公司 | A kind of extraction process of polysaccharides |
| CN109503728A (en) * | 2018-11-26 | 2019-03-22 | 江西省科学院应用化学研究所 | A kind of Gynura procumbens (Lour.) Merr leaf Thick many candies extracting method |
| CN111944072A (en) * | 2020-08-20 | 2020-11-17 | 宋现伟 | A kind of wolfberry polysaccharide extraction method, wolfberry polysaccharide tablet and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103012612A (en) * | 2012-12-14 | 2013-04-03 | 天津大学 | Extraction method of wolfberry polysaccharide |
| CN103333268A (en) * | 2013-07-23 | 2013-10-02 | 草本创想生物科技（成都）有限公司 | Method for preparing lycium ruthenicum polysaccharide |
| CN104231097A (en) * | 2014-08-26 | 2014-12-24 | 陕西盛迈石油有限公司 | Preparation method of lyceum barbarum polysaccharide |
| CN104311690A (en) * | 2014-11-11 | 2015-01-28 | 济南凯因生物科技有限公司 | Extraction method of lycium barbarum polysaccharides |
-
2015
- 2015-12-03 CN CN201510876198.4A patent/CN105367678A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103012612A (en) * | 2012-12-14 | 2013-04-03 | 天津大学 | Extraction method of wolfberry polysaccharide |
| CN103333268A (en) * | 2013-07-23 | 2013-10-02 | 草本创想生物科技（成都）有限公司 | Method for preparing lycium ruthenicum polysaccharide |
| CN104231097A (en) * | 2014-08-26 | 2014-12-24 | 陕西盛迈石油有限公司 | Preparation method of lyceum barbarum polysaccharide |
| CN104311690A (en) * | 2014-11-11 | 2015-01-28 | 济南凯因生物科技有限公司 | Extraction method of lycium barbarum polysaccharides |
Non-Patent Citations (1)
| Title |
|---|
| 吴素萍等: ""酶法提取枸杞多糖的研究"", 《食品科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329399A (en) * | 2018-05-15 | 2018-07-27 | 重庆市洲仨科技发展有限公司 | A kind of extraction process of polysaccharides |
| CN109503728A (en) * | 2018-11-26 | 2019-03-22 | 江西省科学院应用化学研究所 | A kind of Gynura procumbens (Lour.) Merr leaf Thick many candies extracting method |
| CN111944072A (en) * | 2020-08-20 | 2020-11-17 | 宋现伟 | A kind of wolfberry polysaccharide extraction method, wolfberry polysaccharide tablet and preparation method thereof |
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Application publication date: 20160302 |
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