CN100586444C - Method of processing ginseng for medicinal use and composition - Google Patents
Method of processing ginseng for medicinal use and composition Download PDFInfo
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- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
It is expected that the effects of ginseng for medicinal use, i.e., a liver function-improving effect, a hemostatic effect, an antistress effect, an antitumor effect, an aging-retarding effect, an antioxidative effect and so on can be enhanced by a processing method comprising the step of contacting a ginseng plant or its extract with a microorganism or an enzyme and the step of reacting it with an acid or an alkali. The thus processed product is usable as foods, drugs, cosmetics, etc.
Description
Technical field
The present invention relates to the processing method of ginseng for medicinal use and the compositions that obtains with this processing.The application is willing to require priority 2003-339752 number to the spy who filed an application in 30th JIUYUE in 2003, and here cites its content.
Background technology
In recent years, be accompanied by because of life style particularly dietetic life etc. variation (for example, increased the intake of fat food, smoking and drink etc.) reduction of the fat that causes and pressure accumulating in vivo, liver function etc., so-called diseases due to habit disturbances such as diabetes, hyperlipemia, hypertension, gout sharply increase, thereby become social problem.These diseases are along with the development of the state of an illness causes complication, and develop into the disease that heart disease, apoplexy etc. are positioned at cause of the death prostatitis.Therefore, its treatment and prevention are very important.For these diseases, exploitation has the curative of several for example synthetic pharmaceuticals etc.But problems such as side effect are troubling, under the situation that has caused complication, and the multiple medicine of essential further picked-up, thereby the danger that increases the weight of side effect is arranged.Therefore, wait in expectation and have treatment or preventive effect and safe medical material for these diseases or complication.
One of medical material of these requirements can be satisfied, ginseng for medicinal use can be enumerated.Ginseng for medicinal use is continued to use in China from ancient times, widely utilizes as Chinese medicine.Known ginseng for medicinal use has drug effect and preventive effect for arteriosclerosis, hyperlipemia, thrombosis, hypertension, immunologic function reduction, liver function reduction, tumor, hyperglycemia value and pressure etc.Yet these effects can't be enough satisfactory.
As the method that is used to address these problems, thinking has pair ginseng for medicinal use to carry out method for processing.For example there is report to make the method (for example patent documentation 1) of ginseng for medicinal use fermentation with yeast.But the purpose of this method is to improve taste, drug effect improve effect and insufficient.In addition, also have report to make the extract of Radix Ginseng Rubra and the method for glycosylhydrolase effect (for example patent documentation 2), but still not talkatively obtained sufficient drug effect, still leave the leeway of improvement.
Patent documentation 1: the spy opens flat 3-277246 communique
Patent documentation 2: the spy opens the 2002-348245 communique
Summary of the invention
The purpose of this invention is to provide the processing method of the drug effect that is used to improve ginseng for medicinal use and the compositions that obtains with this processing.
In view of the above problems, the inventor etc. study intensively, found that by comprise messenger drug personnel selection participate in microorganism or enzyme contact operation (a) and with the processing of the operation (b) of acid or alkali reaction, the drug effect of significantly improving ginseng for medicinal use and being had, thus finished the present invention.
Promptly, the present invention relates to the processing method of ginseng for medicinal use, it is characterized by, comprise that the messenger drug personnel selection participates in the operation (a) of microorganism or enzyme contact and the operation (b) that the messenger drug personnel selection participates in acid or alkali reaction, after with in the described operation either party ginseng for medicinal use being handled, the described Radix Ginseng of handling is further handled with remaining operation.
The present invention relates to the processing method of ginseng for medicinal use, it is characterized by, can implement operation in the following order and process: the messenger drug personnel selection participates in the operation (a) of microorganism or enzyme contact and the operation (b) that the messenger drug personnel selection participates in acid or alkali reaction.
The present invention relates to the processing method of ginseng for medicinal use, it is characterized by, operation (a) can be to make its operation that contacts with enzyme, and operation (b) can be the operation that makes itself and acid reaction.
The compositions that the present invention relates to obtain with the processing method of ginseng for medicinal use, this processing method comprise to be made its operation (a) that contacts with microorganism or enzyme and makes it and the operation of acid or alkali reaction (b).
The present invention also provides the food that contains above-mentioned composition.
Unessential special operation, the facility of the processing method of ginseng for medicinal use of the present invention.And effect such as the compositions display that is obtained by processing goes out high liver protective effect (liver function improvement effect), arteriosclerosis, prevent to wear out, therefore can be used as food, pharmaceuticals and cosmetics etc. and widely utilize.
Description of drawings
[Fig. 1] is that the acute liver injury model that adopts galactosamine to cause comes the figure of the effect of the active liver protective effect of comparison GPT.
[Fig. 2] is that the acute liver injury model that adopts galactosamine to cause comes the figure of the effect of the active liver protective effect of comparison GOT.
The specific embodiment
The present invention is a purpose with the processing method of the drug effect that is provided for improving ginseng for medicinal use and the compositions that obtains with this processing.As the ginseng for medicinal use that the present invention uses, can enumerate Tienchi-Ginseng (Panax notoginseng (Burk.) F.H.Chen), koryo insam (Panax ginsengC.A.Meyer), panax japonicus (Panax japonicus C.A.Meyer), Radix Panacis Quinquefolii (Panaxquinquefolium L.), Panax vietnamensis Ha et Grushv. (Panax vietnamensis Ha et Grushv.), Panax zingiberensis C. Y. Wu. Et Feng (Panax zingiberensis C.Y.Wu et K.M.Feng), Panax stipuleanatus Tsai et Feng (Panax stipuleanatusH.T.Tsai et K.M.Feng) and Himalaya Radix Ginseng (Panax pseudo-ginseng Wall.subsp.himalaicus Hara) etc.Preferred koryo insam, Tienchi-Ginseng, preferred especially Tienchi-Ginseng.In addition, these ginseng for medicinal use can multiplely be used in combination.
The use position of the ginseng for medicinal use that the present invention uses, the whole positions that can use plant.For example root, rhizome, stem, ramose root, fibrous root, tuber, leaf, flower, fruit, seed, bud, clove etc. can be used alone or as a mixture, root or rhizome can be preferably used.Plant can also pulverize, broken, repair and renovate, severing, roasting is fried in shallow oil and/or processing such as drying and using.
In addition, the ginseng for medicinal use that the present invention uses or by the present invention by the ginseng for medicinal use after operation (a) and/or operation (b) processing, also can be used as the extracting solution that obtains with polar solvent or edible oil extraction plant, its diluent, concentrated solution, extractum, dry itself and the dry thing that obtains uses.Example as the polar solvent that is used to extract can be enumerated water, alcohols such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanols, 1, ethers such as 4-diox, ketones such as acetone, nitriles such as acetonitrile, their mixed liquor etc.Preferred water, methanol, ethanol and their mixed liquor, special preferred water, ethanol and mixed liquor thereof.Example as the edible oil that is used to extract can be enumerated Oleum Brassicae campestris, Oleum helianthi, salad oil, Oleum Glycines, olive oil, Semen Maydis oil, Oleum sesami, safflower oil, Semen Lini oil, perilla oil, Fructus Perillae oil and Oleum Verniciae fordii etc.
As the method for making of ginseng for medicinal use extract, available general known method is carried out.For example can carry out with the following method: add above-mentioned polar solvent behind pulverizing, fragmentation or severing plant or its dry thing and in the material that obtains, under 0 ℃～100 ℃ preferred 20～70 ℃ conditions, place 10 minutes～24 hours preferred 30 minutes～10 hours, remove insoluble matter by operations such as filtration or centrifugalize as required, and concentrate.In addition, also can use non-polar solvens such as ethyl acetate, diethyl ether, hexane to clean as required after, by making with extra care with water extraction.
In processing method of the present invention, can carry out with general known method with the reaction of microorganism.For example, can mix following material and make their reactions: microorganism or in advance on suitable culture medium the plant of cultured microorganism and ginseng for medicinal use, its extract process them with operation (b) after material or its extract is dissolved or hangs material after turbid etc. in water.At this moment, can add suitable carbohydrate (glucose, sucrose etc.), protein (Testa oryzae, wheat bran etc.) etc. as required.Temperature during reaction as long as in the temperature range of microbial activities, just has no particular limits.Preferred 10～50 ℃, more preferably 20～40 ℃.After reaction finishes, can remove insoluble matter by operations such as heat treated and/or filtration or centrifugalize as required, and by making its concentrated or drying obtain desired substance.
As in the processing method of the present invention and the employed microorganism of reaction microorganism, for example can enumerate and belong to aspergillus (Aspergillus japonicus for example, Aspergillus flavus, Aspergilluswentii, Aspergillus niger, Aspergillus niger awamori, Aspergillus oryzae, Aspergillus pulverlentus etc.), Bacillus (Bacillus acidopullalyticus for example, Bacillusamyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus circulans, Bacillus licheniformis, Bacillus polymyxa etc.), Lactobacillus (Lactobacillus brevis for example, Lactobacillus pentoaceticus, Lactobacilluspastorianus, Lactobacillus Lactobacillus casei, Lactobacillus cucumeris, Lactobacillus bulgaricus, Lactobacillus delbrueckii, Lactobacillus jugurti, Lactobacillus helveticus, Lactobacillus plantrum, Lactobacillus acidophilus etc.), the lactobacillus of Rhizopus (for example Rhizopus delemar, Rhizopus oryzae etc.) etc., yeast, microorganisms such as filamentous bacteria.Preferred aspergillus, Bacillus, Lactobacillus, preferred especially aspergillus.In addition, in aspergillus, preferred Aspergillus niger, Aspergillus niger awamori, Aspergillus oryzae, preferred especially Aspergillus oryzae.These microorganisms can be used separately also and can multiplely be used in combination.
Enzyme reaction in the processing method of the present invention can be carried out with general known method.Material after for example processing them or will add the enzyme of necessary amounts in its extract dissolving or the outstanding turbid material after in water at the plant of ginseng for medicinal use, its extract or with operation (b), for example add 0.001～1 weight % with respect to ginseng for medicinal use, preferred 0.05～0.3 weight %, on one side agitation as appropriate more preferably placed for 1 hour～1 preferred 5 hours～5 days of week under 30～50 ℃ the condition at 0 ℃～90 ℃ preferred 20～70 ℃ on one side.Then, make enzyme deactivation, can remove insoluble matter by for example operations such as filtration and/or centrifugalize as required by heat treated, and by it being concentrated or the dry desired substance that obtains.
As the employed enzyme of the enzyme reaction in the processing method of the present invention, for example can enumerate cellulase, hemicellulase, xylanase, Lactose enzyme, pectase, protease, alpha-galactosidase, beta galactosidase, α-Dian Fenmei, beta amylase, hesperidinase and naringinase etc.Preferred Lactose enzyme, xylanase, cellulase, hemicellulase, beta galactosidase and pectase, preferred especially beta galactosidase.These enzymes can merge use with two or more according to reaction temperature.
In the processing method of the present invention can be undertaken by general known method with reaction acid or alkali.For example, with the plant of ginseng for medicinal use, its extract or to process material or the dissolving of its extract after them or be suspended in concentration with operation (a) be in 0.001～50% preferred 0.5～10% the acid or alkali.Then, heat treated 5 minutes～4 hours is preferred 30 minutes～2 hours under 80～150 ℃ of preferred conditions of 100～130 ℃.Then, can remove insoluble matter by neutralization or operations such as filtration and/or centrifugalize as required, remove and desolvate, and by it being concentrated or the dry desired substance that obtains.
There is no particular limitation for employed acid of acid in the processing method of the present invention or alkali reaction or alkali.As acid, for example can enumerate mineral acids such as hydrochloric acid, sulphuric acid, phosphoric acid, nitric acid, acetic acid, citric acid, phosphoric acid, malic acid, succinic acid, fumaric acid, tartaric acid, lactic acid etc. and tablet vinegar (making vinegar and synthetic vinegar etc. of such as grain vinegar, fruit vinegar etc.).Preferred hydrochloric acid, acetic acid, citric acid and tablet vinegar.These acid can only be used a kind of, also can be with two kinds or more kinds of being used in combination.In addition, can exemplify out the alkali of sodium hydroxide, potassium hydroxide, sodium bicarbonate, sodium dihydrogen phosphate etc. as alkali.Preferred sodium hydroxide, potassium hydroxide.These alkali can only use a kind of, also can be with two kinds or more kinds of being used in combination.In addition, in the processing method of the present invention and reaction acid or alkali reaction, with the reaction of acid in, the preferred 1.0＜pH of pH value＜4.5 of acid, more preferably 2.0＜pH＜4.0, further preferred 2.0＜pH＜3.8.In addition, with the reaction of alkali in, the preferred 7.5＜pH of the pH value of alkali＜11.0, more preferably 8.0＜pH＜10.0, further preferred 8.5＜pH＜10.0.
The processing method of ginseng for medicinal use of the present invention comprises that the messenger drug personnel selection participates in the operation (a) of microorganism or enzyme contact and the operation (b) that the messenger drug personnel selection participates in acid or alkali reaction.Its order can be selected arbitrarily, but preferably according to the messenger drug personnel selection participate in microorganism or enzyme contact operation (a) and with the such order of operation (b) of acid or alkali reaction, react successively.In addition, in process separately, the viewpoint from the drug effect effect of improving ginseng for medicinal use preferably makes its reaction that contacts with enzyme in operation (a), preferably makes itself and acid reaction in operation (b).Thus, more preferably, react successively according to making its operation that contacts with enzyme, making the such order of operation of itself and acid reaction.
By the compositions that processing method of the present invention obtains, can further make with extra care the back and use with extraction and/or chromatographic column.Extraction can be carried out with said method.Can followingly carry out with the refining of chromatographic column: for example with composition dissolves of the present invention or be suspended in purify waste water in after, join in the chromatographic column of macroporous resin, clean unwanted material with purifying waste water, follow ethanol elution, can obtain desired substance by concentrating this eluent with 70～90%.(for example in order to decolour) as required, also can further make ethanol elution pass through ion exchange resin column after, the ethanol elution with 70～90%.Can obtain compositions of the present invention by concentrating this eluent.
Macroporous resin used in the present invention for example is meant by polymeric porous polymers such as styrene, diphenyl benzene ethylene or α-Jia Jibingxisuan esters, is meant that the fine continuous hole that has big surface area, is called as pore develops into the material of polymer particle inside.By this structural feature this type of material adsorb organic compound efficiently as can be known, and be used to concentrating of natural goods and tunning etc. refining in.As macroporous resin, can use general known material.For example can use Amberlite XAD-1, Amberlite XAD-2 (more than be ROHM AND HASS corporate system), DIAIONHP20, DIAION HP21, SEPABEADS SP825, SEPABEADS SP850, SEPABEADS SP207, DIAION HP2MG (more than be Mitsubishi chemical Co., Ltd's system) etc.
The ion exchange resin of Shi Yonging in the present invention, essential special material can use general known material.Example as ion exchange resin, storng-acid cation exchange resin, weak-acid cation-exchange resin, strong-base anion-exchange resin, weak-base anion-exchange resin, hybrid resin be can enumerate, storng-acid cation exchange resin, weak-acid cation-exchange resin and hybrid resin enumerated especially.
The refining thing of compositions, its extract or chromatographic column that is obtained by processing method of the present invention has various drug effects (prevention and therapeutic effect).For example have liver protective effect (liver function improvement effect), arteriosclerosis, prevent to wear out, antitumor action, hyperlipidemia, antithrombotic, the effect of bringing high blood pressure down, improve immunologic function, the effect of blood sugar lowering value, analgesic activity, cardiotonic, antiinflammatory action, improve effects such as blood flow of the tip effect, anastalsis, antioxidation etc. and resistance to compression.Thereby can be used for their treatment.The refining thing of compositions of the present invention, its extract and chromatographic column also can be used in combination mutually.And, also the multiple combination of compositions that obtains with method of the present invention can be used.
The refining thing of compositions, its extract or chromatographic column by processing method of the present invention obtains can be used as food, pharmaceuticals, cosmetics or composition for oral cavity (toothpaste) according to purposes and uses.
When using, can cooperate other food materials to the present composition as food.For example, to provide liver protective effect (liver function improvement effect) when being purpose, can cooperate propolis, Radix Curcumae, Herba Silybi mariani, Semen Myristicae, Fructus Lycii, isoflavone, Lac regis apis, lactoferrin and extract thereof, liver to extract extractum etc.When providing antioxidant effect to be purpose, can cooperate astaxanthin, Semen Vitis viniferae (procyanidin), vitamin E, polyphenol, catechuic acid etc.When providing arteriosclerosis to be purpose, can cooperate Radix Glycyrrhizae, Cortex Moutan, the Radix Astragali, Radix Salviae Miltiorrhizae, Semen Vitis viniferae (procyanidin proanthocyanidin), core of Caulis et Folium Lini seed etc. and extract thereof.When providing antithrombotic to be purpose, can cooperate nattokinase, core of Caulis et Folium Lini seed etc. and extract thereof.When being purpose, can cooperate Flos Caryophylli Lagerstroemia indica L., Sarah west Ya Bu Longjia (サ ラ シ ア オ Block ロ Application ガ), Sarah Xi Yalei to carry ball and draw (サ ラ シ ア レ テ イ キ ュ ラ one タ), sweet potato, Ya Gong (ヤ one コ Application), the Fructus Kochiae, Ganoderma and extract, former cyanidin (procyanidin) etc. with the blood sugar lowering value.When providing hypotensive effect to be purpose, can cooperate γ-An Jidingsuan, sardine peptide, loose fish peptide, tuna peptide, propolis, feather Caulis et Folium Brassicae capitatae, Ganoderma, noni fruit (ノ ニ), Semen Vitis viniferae (procyanidin) etc. and extract thereof.These materials that can cooperate can only cooperate a kind of, also can make up two kinds or more kinds of the cooperation.
The food material that can be matched with in the present composition is not limited to above-mentioned material, can suitably cooperate according to its purposes, purpose.In addition, as required, excipient can be cooperated, for example cellulose, crystalline cellulose, lactose, sucrose fatty acid ester, lactose, reduction maltose, alphalysed starch, PINEFIBRE, dextrin, PINEDEX etc. can be suitably cooperated.
As the form of food, for example can use the semifluid food of solid food, butyrous or fruit jam shape, gelatinous food, tablet, powder, scrotiform tablet, can be filled in addition in soft capsule or the hard capsule and use.In this case, when the capsule tunicle is gelatin, for example insoluble in order to prevent tunicle, with respect to the filling content total amount of wafer, can cooperate phosphatidyl ethanolamine and contain its soybean lecithin, yolk lecithin or amino sugar (glucosamine, N-acetylglucosamine, galactosamine, N-acetylgalactosamine, N-n acetylneuraminic acid n etc. have amino saccharide or be the chitosan of the polymer of glucosamine) 0.05～20 quality % and organic acid (the inositol monophosphate derivant of phytic acid etc., citric acid etc.) 0.05～20 quality %.
The compositions that will be obtained by processing method of the present invention, the refining thing of its extract or chromatographic column can be with general known method manufacturing as the soft wafer of filling content.For example, with gelatin soluble in water (this moment can heat.Perhaps can add plasticizer, antiseptic, aminoacid etc. as required.), being dried to moisture by the solution that will obtain is 5～20% more preferably 7～15% to obtain the capsule tunicle.The filling content of the excipient that is added with the present composition, soybean lecithin, phytic acid and adds as required can be filled in this capsule tunicle with forms such as aqueous, suspension, pasty state, Powdered or graininess, perhaps can be formed and make by will fill the content bag with above-mentioned capsule tunicle.
By the refining thing of compositions, its extract or chromatographic column that processing method of the present invention obtains, its use level contained in food can be selected arbitrarily.
In the time of will being used as cosmetics by the refining thing of compositions, its extract or chromatographic column that processing method of the present invention obtains, in the scope of not damaging effect of the present invention, can be matched with in the cosmetic according to its application target, can cooperate preferred 0.00001～5.0 weight % of 0.000005～10.0 weight % in whole cosmetics as solid constituent.And, can also suitably select vitamin C, vitamin E, astaxanthin, Semen Vitis viniferae (procyanidin), collagen, chrondroitin and glucosamine to wait as required and be used.These materials can use a kind of, also can be with two kinds or more kinds of being used in combination.
The compositions that will obtain by processing method of the present invention; when the refining thing of its extract or chromatographic column is used as the shampoo composition; in the scope of not damaging effect of the present invention, can cooperate the ionic surfactant according to its application target; nonionic surfactant; suds booster; thickening agent; powder body; abrasive cleaner; antioxidant; antiseptic; surfactant; wetting agent; UV absorbent; polymer class; inorganic Margarita agent; the antidandruff agent; chelating agen; pH adjusts agent; electrolyte; coloring agent; spice; the beauty treatment of protection scalp or Hair grooming becomes to grade; other the composition that generally is used for cosmetic.These compositions can use a kind of, also can be with two kinds or more kinds of being used in combination.Can select arbitrarily by the refining thing contained use level in shampoo of compositions, its extract or chromatographic column that processing method of the present invention obtains.
The refining thing of compositions, its extract or the chromatographic column that is obtained by processing method of the present invention can cooperate grinding agent, wetting agent, binding agent, foaming agent, other the composition that generally is used for composition for oral cavity (toothpaste) during as the composition of composition for oral cavity (toothpaste etc.).These compositions can use a kind of, also can be with two kinds or more kinds of being used in combination.When the refining thing of compositions, its extract or the chromatographic column that is obtained by processing method of the present invention was used as composition for oral cavity, contained therein use level can be selected arbitrarily.
The refining thing of compositions, its extract or the chromatographic column that will be obtained by processing method of the present invention is during as medicine, can be used to inject, the admissible carrier in pharmacy of the oral administration of non-oral administration, solid or liquid form such as per rectum etc. joins prescription as compositions.These compositions can use a kind of, also can be with two kinds or more kinds of being used in combination.By the refining thing of compositions, its extract or chromatographic column that processing method of the present invention obtains, its use level contained in medicine can be selected arbitrarily.
As the form of the compositions of the present invention that is used for injection, can enumerate admissible sterilized water, non-aqueous solution, suspension or emulsion in pharmacy.As the example of suitable nonaqueous carrier, diluent, solvent or the vehicle that can in above-mentioned, use, can enumerate the organic ester that propylene glycol, Polyethylene Glycol, Fructus Canarii albi wet goods vegetable oil, ethyl oleate etc. can be injected.The compositions that is used for injection like this can also contain co-adjuvants such as antiseptic, wetting agent, emulsifying agent, dispersant.These compositionss can be sterilized in the following way: for example the filter with retain bacteria filters, perhaps before be about to using, sneak into biocide with the form that can be dissolved in the aseptic solid composite in other the medium of the injection of can sterilizing of aquesterilisa or some.
As being used for peroral administration solid preparation, can enumerate wafer, tablet, pill, powder, granule etc.In modulation during this solid preparation, generally the non-activated thinner with for example sucrose of chemical compound of the present invention and at least one, lactose, starch etc. mixes.Can further contain in the said preparation in common preparationization and except non-activated thinner, to append material, for example smoothing preparation (for example magnesium stearate etc.).Under the situation of wafer, tablet and pill, can also comprise buffer agent.Can also further impose the enteric solubility tunicle to tablet and pill.
As being used for peroral administration liquid preparation, can enumerate the normally used non-activated thinner of those skilled in the art, for example contain admissible Emulsion, solution, suspending agent, syrup, elixir etc. in the pharmacy of water.Except described non-activated thinner, can also in compositions, cooperate co-adjuvants such as wetting agent, emulsifying agent, suspending agent, sweeting agent, flavoring agent, flavouring agent.Be used for preferably except chemical compound of the present invention, also containing the excipient of cocoa butter, suppository wax etc. under the preparation situation of per rectum administration.
The dosage of the refining thing of compositions, its extract or the chromatographic column that obtains by processing method of the present invention, because of during the character of the refining thing of compositions, its extract or chromatographic column that given, route of administration, the treatment desired, and other principal elements be affected.General every day about 0.1～100mg/kg, preferred especially about 0.5～50mg/kg.In addition, according to hope, this daily dose can be divided into 2～4 administrations.
Embodiment
Below with embodiment the present invention is described, but they do not limit the present invention.
Embodiment 1
Taking by weighing 100g Tienchi-Ginseng powder and 10g is the enzyme agent Lactose enzyme F " AMANO " (the wild ENZYME in sky Co., Ltd. system) of origin with Aspergillus oryzae, make its dissolving, suspension to wherein adding 2.5L water, put into 50 ℃ calorstat, make its vibration every now and then on one side, Yi Bian make its reaction 5 days.Add acetic acid then and become 1v/v% to acetic acid concentration, heat treated (120 ℃, 40 minutes) makes its reaction, makes enzyme deactivation simultaneously, with the lyophilizing of this reactant liquor former state, promptly gets brown powder 113g.
Embodiment 2
Taking by weighing 100g koryo insam and 10g is the enzyme agent Lactose enzyme F " AMANO " (the wild ENZYME in sky Co., Ltd. system) of origin with Aspergillus oryzae, make its dissolving, suspension to wherein adding 2.5L water, put into 50 ℃ calorstat, Yi Bian make its vibration every now and then, Yi Bian make its reaction 5 days.Add acetic acid then and become 1v/v% to acetic acid concentration, heat treated (120 ℃, 40 minutes) makes its reaction, makes enzyme deactivation simultaneously, with the lyophilizing of this reactant liquor former state, promptly gets brown powder 98g.
Embodiment 3
Taking by weighing 100g Tienchi-Ginseng powder and 5g is the enzyme agent cellulase Y-NC (YAKULT pharmaceutical industries Co., Ltd. system) of origin with Aspergillus niger, to wherein adding 2.5L water, put into 50 ℃ calorstat, Yi Bian make its vibration every now and then, Yi Bian make its reaction 5 days.Then, add 5% hydrochloric acid 10mL, heat treated (120 ℃, 40 minutes) makes its reaction, makes enzyme deactivation simultaneously.With the lyophilizing of this reactant liquor former state, promptly get brown powder 108g.
Embodiment 4
Taking by weighing 100g Tienchi-Ginseng powder and 10g is the enzyme agent filling wood powder HC (the anxious Bioindustry of slope Co., Ltd. system) of origin with Aspergillus niger, to wherein adding 2.5L water, put into 50 ℃ calorstat, Yi Bian make its vibration every now and then, Yi Bian make its reaction 5 days.Add the 5g citric acid then, heat treated (120 ℃, 40 minutes) makes its reaction, makes enzyme deactivation simultaneously.With the lyophilizing of this reactant liquor former state, promptly get brown powder 115g.
Embodiment 5
The powder 5.0g that will obtain in embodiment 1 is dissolved among the 100ml that purifies waste water.This solution is added in the chromatographic column of DIAION HP20 (Mitsubishi chemical Co., Ltd's system) 200CC, washes out unwanted material with purifying waste water of 10 times of amounts of chromatographic column volume.Then use 70% ethanol elution of 5 times of amounts of chromatographic column volume.Reclaim eluent, and then after making it pass through ion exchange resin DIAION SK104 (Mitsubishi chemical Co., Ltd's system), with 70% ethanol elution of chromatographic column volume equimultiple.Concentrate eluant promptly gets pressed powder 400mg through drying under reduced pressure.
(test example 1)
For the compositions that relatively obtains in embodiment 1 and the liver protective effect of untreated Tienchi-Ginseng, the hepatic injury model that brings out with the galactosamine of rat experimentizes.The hepatic injury model that this galactosamine brings out, consider with clinical relevant situation under, known as general immunologic hepatic injury model.In addition, if hepatocyte is undermined, aspartate transaminase in the cell (GOT) and alanine aminotransferase enzymes such as (GPT) will dissociate in blood, and the activity of these enzymes rises rapidly.That is, these GOT in the blood and GPT activity are known as the index of representing liver function impairment, thereby have carried out following experiment.
To Wistar is after preparation that male rat (6 week age) carried out for 1 week is raised, to be used for experiment.Experiment component is following 4 groups: normal group, matched group, Tienchi-Ginseng 3.0g/kg organize and Tienchi-Ginseng processed thing (embodiment 1) 3.3g/kg organizes, and normal group is made as 5, and other groups are made as 1 group 10.Giving medicinal liquid all is that capacity per os with 20mL/kg gives rat, and matched group is the distilled water that per os gives equivalent.For other 3 groups that remove normal group, 1 day 1 time per os gives analyte in 7 days, and vein gave galactosamine 400mg/kg in the end one day, caused hepatic injury.Giving galactosamine after 24 hours, take a blood sample from abdominal aorta, with GPT and the GOT activity in the TRANSAMINASECII TEST WAKO mensuration blood plasma.For check, after having carried out the one-way layout dispersion analysis, carry out the multiple comparisons check of Tukey.It the results are shown among Fig. 1 and Fig. 2.The Tienchi-Ginseng processed thing of embodiment 1 is compared with Tienchi-Ginseng, has obtained the active significant inhibition effect of GPT and GOT (P＜0.01), thereby confirms to have the liver protective effect.(soft capsule Production Example)
The gelatin solution that will contain gelatin 43 weight %, glycerol 17 weight % and water 40 weight % is after dissolving, deaeration under 80 ℃, left standstill about 10 hours, adopt rotary soft capsule filling machine (OVAL 5 types), fill perilla oil 180mg, the soybean lecithin 2mg, the phytic acid 18mg that are dissolved with embodiment 1 powder 150mg.After capsule is filled, temperature be 27 ℃, humidity be 50% or lower condition under, be dried to tunicle moisture and reach 8%.Make soft wafer.
(reference example 1)
Take by weighing 100 gram Tienchi-Ginseng powder,, make its dissolving, suspension to wherein adding 2.5L water.Further add acetic acid and become 1v/v% to acetic acid concentration, heat treated (120 ℃, 40 minutes) makes its reaction, with the lyophilizing of this reactant liquor former state, promptly gets brown powder 101g.
(reference example 2)
Taking by weighing 100g Tienchi-Ginseng powder and 10g is the enzyme agent Lactose enzyme F " AMANO " (the wild ENZYME in sky Co., Ltd. system) of origin with Aspergillus oryzae, make its dissolving, suspension to wherein adding 2.5L water, put into 50 ℃ calorstat, make its vibration every now and then on one side, Yi Bian make its reaction 5 days.Carry out heat treated (105 ℃, 10 minutes) then, make enzyme deactivation,, promptly get brown powder 110g the lyophilizing of this reactant liquor former state.
(test example 2)
In order to study the liver protective effect of the compositions that obtains and unprocessed Tienchi-Ginseng, reference example 1 compositions, reference example 2 compositionss in embodiment 1, the hepatic injury model that hepatic injury model that brings out with the galactosamine of rat and carbon tetrachloride bring out experimentizes.The hepatic injury model that known galactosamine brings out is as the viral hepatitis model, and the hepatic injury model that carbon tetrachloride brings out is very high as the dependency of toxic hepatitis model and clinical effectiveness.
To Wistar is that the rat that grew up to for 7 ages in week was used for the hepatic injury model experiment that galactosamine brings out after preparation that male rat (6 or 7 week age) carried out for 1 week was raised, and the rat that grew up to for 8 ages in week is used for the hepatic injury model experiment that carbon tetrachloride brings out.Experiment component is following 6 groups: normal group, matched group, undressed Tienchi-Ginseng 3.0g/kg organize, reference example 1 compositions 3.0g/kg organizes, reference example 2 compositions 3.3g/kg organize and embodiment 1 compositions 3.3g/kg organizes, normal group is made as 3, and other groups are made as 1 group 10.Giving medicinal liquid all is that capacity per os with 20mL/kg gives rat, and the matched group per os gives the distilled water of equivalent.In the experiment of the hepatic injury model that adopts galactosamine to bring out, for except other 5 groups of normal group, 1 day 1 time per os gives analyte in 4 days, and vein gave galactosamine 400mg/kg in the end one day, caused hepatic injury.Equally, in the experiment of the hepatic injury model that adopts carbon tetrachloride to bring out, for except other 5 groups of normal group, 1 day 1 time per os gives analyte in 4 days, and intraperitoneal gave carbon tetrachloride 0.75ml/kg in the end one day, caused hepatic injury.Giving galactosamine (or carbon tetrachloride) after 24 hours, take a blood sample from abdominal aorta, with the GPT activity in the TRANSAMINASE C II TEST WAKO mensuration blood plasma.For check, carried out the one-way layout dispersion analysis after, the multiple comparisons that carries out Tukey detects.Compare with matched group, confirm not have significant poor markers be designated as *, confirming has significant poor markers to be designated as zero (p＜0.05), confirming has significant especially poor markers to be designated as ◎ (P＜0.01), it the results are shown in the table 1.Embodiment 1 compositions and unprocessed Tienchi-Ginseng and reference example 1 compositions, reference example 2 compositionss are compared, and have confirmed all to have in two kinds of models the active effect that rises of strong inhibition GPT.Can confirm that by above embodiment 1 compositions has strong liver protective effect.
Table 1
| Carbon tetrachloride brings out the hepatic injury model | Galactosamine brings out the hepatic injury model | |
| Undressed Tienchi-Ginseng reference example 1 compositions reference example 2 compositions embodiment 1 compositions | ○ ○ ◎ ◎ | × × × ◎ |
The possibility of industrial application
Can reach the various pharmacological effects that the enhancing ginseng for medicinal use has by the present invention.
Claims (14)
1, the processing method of ginseng for medicinal use is characterized by, and comprises that the messenger drug personnel selection participates in enzyme and participates in acid 80～150 ℃ of operations (b) of reacting down in 0～90 ℃ of operation (a) that contacts down and messenger drug personnel selection,
After with in the described operation either party ginseng for medicinal use being handled, the described Radix Ginseng of handling is further handled with remaining operation.
2, the processing method of ginseng for medicinal use as claimed in claim 1 is characterized by, and processes by such order: the messenger drug personnel selection participates in the operation (a) of enzyme contact and the operation (b) of messenger drug personnel selection participation acid reaction.
3, the processing method of ginseng for medicinal use as claimed in claim 1 is characterized by, and described ginseng for medicinal use is the plant of ginseng for medicinal use or the extract of ginseng for medicinal use.
4, the processing method of ginseng for medicinal use as claimed in claim 3, it is characterized by, described ginseng for medicinal use is the extract of ginseng for medicinal use, and described extract is from the diluent of the extracting solution that obtains, aforementioned extracting solution with the plant of polar solvent or edible oil extraction ginseng for medicinal use, concentrated solution, suspension, extractum, dry aforementioned extracting solution and select the dry thing that obtains.
5, the processing method of ginseng for medicinal use as claimed in claim 1 is characterized by, and the time of the described operation that contacts with enzyme (a) was 1 hour～1 week, and the time of the operation (b) of described and acid reaction is 5 minutes～4 hours.
6, the processing method of ginseng for medicinal use as claimed in claim 1 is characterized by, and ginseng for medicinal use is a Tienchi-Ginseng.
7, a kind of compositions with liver protective effect is characterized by, and obtains with processing method as claimed in claim 1.
8, a kind of compositions with liver protective effect is characterized by, by obtaining with the refining compositions as claimed in claim 7 of at least one method of extraction and chromatographic column.
9, a kind of food is characterized by, and contains the compositions that the described processing method of useful claim 1 obtains.
10, a kind of pharmaceuticals is characterized by, and contain compositions as claimed in claim 7.
11, pharmaceuticals as claimed in claim 10, it is a hepatoprotective.
12, a kind of cosmetics is characterized by, and contain the compositions that the described processing method of useful claim 1 obtains.
13, a kind of soft wafer is characterized by, and contains compositions as claimed in claim 7.
14, the processing method of ginseng for medicinal use as claimed in claim 1 is characterized by, and also comprises operation with ginseng for medicinal use pulverizing, fragmentation and/or severing before in operation (a) or operation (b).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| JP2003339752 | 2003-09-30 | ||
| JP339752/2003 | 2003-09-30 |
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| CN1859921A CN1859921A (en) | 2006-11-08 |
| CN100586444C true CN100586444C (en) | 2010-02-03 |
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| CN200480028083A Expired - Fee Related CN100586444C (en) | 2003-09-30 | 2004-09-29 | Method of processing ginseng for medicinal use and composition |
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| JP (1) | JP4767686B2 (en) |
| CN (1) | CN100586444C (en) |
| HK (1) | HK1096042A1 (en) |
| WO (1) | WO2005030235A1 (en) |
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| CA2676864C (en) * | 2007-02-02 | 2016-07-05 | Pos Pilot Plant Corp. | Extraction of phytochemicals by enzymatic hydrolysis |
| WO2009063987A1 (en) * | 2007-11-15 | 2009-05-22 | Wakunaga Pharmaceutical Co., Ltd. | Novel use of processed ginseng |
| CN102006878A (en) * | 2008-02-19 | 2011-04-06 | 优力竟株式会社 | Leaves extract of panax sp., a process of making the same and uses thereof |
| JP5232726B2 (en) * | 2008-06-04 | 2013-07-10 | 森川健康堂株式会社 | Method for producing royal jelly having blood pressure lowering action |
| CN102149399A (en) | 2008-09-09 | 2011-08-10 | 狮王株式会社 | Method for producing a composition with a high content of sapogenin |
| EP2432891B1 (en) * | 2009-05-19 | 2018-04-04 | Il Hwa Co., Ltd. | Methods for preparing a fermented ginseng concentrate or powder |
| JP5546851B2 (en) | 2009-12-21 | 2014-07-09 | ライオン株式会社 | Anemia ameliorating agent and anemia ameliorating composition |
| KR100992800B1 (en) | 2010-05-14 | 2010-11-08 | 주식회사 지씨에이치앤피 | A process for preparing novel processed ginseng or extract thereof showing increased amount of minor ginsenosides |
| KR100997054B1 (en) * | 2010-06-11 | 2010-11-30 | 홍진석 | Fermentated woods grown ginseng roots and fermentation method for the same |
| CN102091022A (en) * | 2010-09-08 | 2011-06-15 | 成进学 | Nanometer selenium-VE (vitamin E) anti-wrinkle skin-smoothing cream |
| KR101458274B1 (en) * | 2012-10-08 | 2014-11-04 | 주식회사 한국인삼공사 | Cosmetic Composition Comprising Fermented Ginseng Extracts |
| CN103284940B (en) * | 2013-06-28 | 2014-08-06 | 上海生态美日化有限公司 | Saccharomycetes fermented traditional Chinese medicine composition, as well as preparation method and application thereof |
| CN103463152A (en) * | 2013-08-11 | 2013-12-25 | 吉林农业大学 | Method for preparing ginseng pulp through utilizing glucoamylase |
| CN103446208B (en) * | 2013-08-11 | 2018-06-19 | 吉林农业大学 | A kind of method that ginseng pulp is prepared using cellulase |
| CN103446209B (en) * | 2013-08-11 | 2016-04-13 | 吉林农业大学 | A kind of method preparing ginseng pulp based on α-amylase |
| WO2015029134A1 (en) * | 2013-08-27 | 2015-03-05 | 金氏高麗人参株式会社 | Ginsenoside composition |
| EP3854409A1 (en) | 2013-08-30 | 2021-07-28 | Green Cross Wellbeing Corporation | Composition for preventing and treating cancer-related fatigue, containing processed ginseng powder or processed ginseng extract having increased ginsenoside constituent |
| KR101591251B1 (en) * | 2013-09-26 | 2016-02-03 | 샘표식품 주식회사 | Ginseng or mountain ginseng containing improved ginsenoside and aglycon, and method for processing thereof |
| KR102073691B1 (en) * | 2016-01-27 | 2020-02-05 | 샘표식품 주식회사 | Ginseng or mountain ginseng containing improved ginsenoside and aglycon, and method for processing thereof |
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| JPH0683656B2 (en) * | 1985-11-26 | 1994-10-26 | 日東電工株式会社 | Method for producing ginseng cream |
| JPH05161481A (en) * | 1991-12-11 | 1993-06-29 | Nitto Denko Corp | Modified ginseng extract its production and its use |
| JP3238192B2 (en) * | 1992-04-01 | 2001-12-10 | 江崎グリコ株式会社 | Method for concentrating and purifying useful components in animals and plants |
| JPH11169135A (en) * | 1997-12-10 | 1999-06-29 | Nagaoka Jitsugyo Kk | Medicinal ginseng processing sour liquid and its production |
| JP4549625B2 (en) * | 2002-01-05 | 2010-09-22 | 株式會社アモーレパシフィック | Finely emulsified particles containing ginseng saponin metabolites as active ingredients, a method for producing the same, and a cosmetic composition for preventing skin aging containing the same |
-
2004
- 2004-09-29 CN CN200480028083A patent/CN100586444C/en not_active Expired - Fee Related
- 2004-09-29 WO PCT/JP2004/014265 patent/WO2005030235A1/en active Application Filing
- 2004-09-29 JP JP2005514260A patent/JP4767686B2/en not_active Expired - Lifetime
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2007
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Also Published As
| Publication number | Publication date |
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| JP4767686B2 (en) | 2011-09-07 |
| WO2005030235A1 (en) | 2005-04-07 |
| CN1859921A (en) | 2006-11-08 |
| JPWO2005030235A1 (en) | 2007-11-15 |
| HK1096042A1 (en) | 2007-05-25 |
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