KR101444385B1 - Microorganism cultivation device with medium inserting part - Google Patents

Abstract

The present invention is characterized in that after sterilizing the feed section by simply injecting steam into the feed section of the medium and discharging the steam to the outside, the feed section of the medium is connected to the discharge section of the feed tube, A microorganism culture apparatus comprising a culture tank having a sterilization medium input section for allowing a user to quickly perform a sterilization operation and a culture tank provided with a culture medium input part on one side of the culture tank, A vertical communicating tube having a threaded portion formed on an outer circumferential surface thereof; A first opening / closing valve fastened to the upper portion of the vertical communicating pipe; A vertical connection pipe connected to an upper portion of the first opening / closing valve and having a screw portion formed on an outer circumferential surface of the upper portion; A second opening / closing valve fastened to the upper portion of the vertical connection pipe; An inlet pipe connected to an upper portion of the second opening / closing valve; A charging pipe cap for blocking the upper portion of the charging pipe; A steam supply pipe connected to an upper portion of the vertical connection pipe and having a third open / close valve; A steam discharge pipe connected to a lower portion of the vertical connection pipe and having a fourth open / close valve; .

Description

Technical Field [0001] The present invention relates to a microorganism cultivation apparatus having a sterilization medium input part,

The present invention relates to a microorganism culture apparatus having a sterilization medium input unit, more particularly, to a sterilization apparatus for a microorganism culture apparatus having a sterilization medium input unit and a method of sterilizing the medium input unit by simply injecting steam into the medium insertion unit, To a microorganism culturing apparatus having a sterilizing medium feeding section for rapidly feeding a medium by pouring a liquid medium into the microorganism culturing apparatus.

In recent years, technologies for utilizing microorganisms in industry have been widely developed. As a method of using these microorganisms, microorganisms are cultured and sprayed on the soil to promote the production of crops, to prevent pests and diseases, to promote the growth of livestock, And contributes to obtaining high quality meat quality.

In the aquaculture industry, it can also be used for water purification, organic waste fermentation, and industrial waste purification. These microorganisms are also effective in reducing odor and pollution.

Therefore, in order to utilize microorganisms easily and stably, conventionally, a microorganism culture apparatus is used. The conventional microorganism culturing apparatus is configured to be capable of stirring and air injection as well as feeding of the medium.

A conventional conventional microorganism culture apparatus includes a culture tank in which a mixture (culture liquid) of a culture medium and a seed culture (hereinafter, referred to as "microorganism") is stored, a culture tank disposed in the culture tank, A motor for transmitting power by the stirring means, and an air supply unit installed on a bottom surface of the culture tank.

At the upper part of the culture tank, an inoculation port (hereinafter referred to as "medium feed port") is provided for inoculating the medium and seeds. The culture medium inlet is composed of a charging tube having a thread formed on the outer peripheral surface thereof and a cap which is fastened to the charging tube and blocks the upper part of the charging tube.

Conventionally, when the culture medium is introduced into the culture tank through the culture medium inlet, the culture medium is flame-treated through the ignition of alcohol around the inlet of the injection tube so as to block contaminants in the air, Is used.

However, when the culture medium is flame-treated in the flame-treated surroundings as described above, there is a risk of burns, contamination of the periphery of the injection tube, contamination of microorganisms in the culture tank, I had a problem.

In order to solve such a problem, Korean Patent Registration No. 0767569 "incubator" has been proposed in the past.

However, such an incubator requires a lot of time for inoculation because the inoculation port of the seedling must be connected to the inoculation line, the sterilization supply line, and the steam supply line of the seedling inoculation line, and the structure of the medium feeding part is complicated, .

Korean Patent No. 0767569 "incubator" (2007.10.09)

Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art,

It is an object of the present invention to sterilize the medium feeding section by simply injecting steam into the medium feeding section and discharging the steam to the outside and then connecting the medium feeding section and the discharging section of the discharging cylinder immediately after pouring the liquid medium, The sterilizing structure of the medium feeding section can be simplified to reduce the manufacturing cost of the medium feeding section and the steam feeding pipe for discharging the steam to the atmosphere into the medium feeding section can be used to sterilize the medium feeding section And a sterilization medium feeding part for rapidly discharging steam to the outside to cool the feeding part of the microorganism.

Another object of the present invention is to provide a microorganism culturing apparatus which allows a culture medium to be easily put upside down.

Another object of the present invention is to provide a microorganism culturing apparatus having a sterilization medium feeding section which allows the culture medium to be introduced into a culture tank only through a process of inverting the culture cylinder without opening the discharge port lid of the culture cylinder.

Another object of the present invention is to provide a sterilization apparatus for sterilizing a sterilization medium having a sterilization medium feeding section for allowing the height of the perforated circular plate to be adjusted according to the height of the outlet of the sterilization apparatus, And to provide a microorganism culture apparatus.

It is a further object of the present invention to provide a method of manufacturing a microorganism by connecting a discharging portion of a discharging container with a discharging portion connecting the discharging portion connecting tube with the discharging portion connecting portion, sterilizing the discharging portion connecting tube with steam, And a sterilization medium feeding part for feeding the medium in a simpler and quicker manner.

In order to achieve the above object, a microorganism culture apparatus having a sterilization medium input section according to the present invention includes a culture tank provided with a culture medium input section on one side of the microorganism culture apparatus, A vertical communicating tube formed on the upper plate of the tank so as to protrude upward and having an outer peripheral surface formed with a threaded portion; A first opening / closing valve fastened to the upper portion of the vertical communicating pipe; A vertical connection pipe connected to an upper portion of the first opening / closing valve and having a screw portion formed on an outer circumferential surface of the upper portion; A second opening / closing valve fastened to the upper portion of the vertical connection pipe; An inlet pipe connected to an upper portion of the second opening / closing valve; A charging pipe cap for blocking the upper portion of the charging pipe; A steam supply pipe connected through the outer surface of the vertical connection pipe and having a third opening / closing valve; A steam discharge pipe connected to the outer surface of the vertical connection pipe through the fourth opening / closing valve; .

The inlet pipe of the device for culturing a microorganism having a sterilization medium inlet portion according to the present invention is characterized in that the inlet pipe comprises an expansion portion formed in the upper portion of the injection pipe and a peripheral portion formed in the periphery of the inside of the expansion portion, And a screw portion formed on an outer peripheral surface of the expanded portion, wherein the injection tube stopper is fastened and fixed to the screw portion.

Further, in the "microorganism culture apparatus with a sterilization medium input section" according to the present invention, the culture medium input section is provided inside the expansion section of the injection tube, and a plurality of vertical through holes are formed around the center, A perforated circular plate having a discharge recess groove formed therein and having a pointed perforation needle protruding therefrom; And further comprising:

Further, in the "apparatus for culturing a microorganism having a sterilization medium input section" according to the present invention, the expanded section further includes a tab section formed on the inner circumferential surface thereof, and the perforated circular plate further includes a thread part formed on an outer circumferential surface of the perforated circular plate, .

Further, in the "apparatus for culturing a microorganism having sterile medium input section" according to the present invention, the inlet tube further comprises a flange protruding outward from an upper end of the inlet tube, A connection pipe flange formed on one end portion of the flange in close contact with the upper surface of the flange of the flange and having a connection lid for closing the discharge port of the discharge pipe and being bent, And a flange clamp connecting the flange and the connection flange.

The method of the present invention as described above is characterized in that after sterilizing the medium input part by simply injecting steam into the medium input part and discharging the steam to the outside, immediately connecting the medium input part and the discharge part of the discharge cylinder, The present invention relates to a sterilizing apparatus for sterilizing a sterilization medium in a sterilization apparatus for sterilization of a sterilization chamber of a sterilization apparatus, The steam used for the steam is directly discharged to the outside, so that the feeding portion is cooled quickly.

In addition, the present invention has an effect that the medium can be simply put in the upside-down position.

In addition, the present invention has an effect that the culture medium can be introduced into the culture tank only through the process of inverting the culture medium cylinder without opening the discharge port lid of the culture medium cylinder.

In addition, the present invention has an effect that the height of the perforated circular plate is adjusted according to the height of the discharge port of the discharge tube, so that the discharge port lid of the discharge cylinder can be drilled irrespective of the discharge port height of the discharge cylinder.

In addition, the present invention relates to a method for preparing a culture medium by connecting a discharging portion of a discharging container with a discharging portion connecting tube through a discharging tube connecting portion, sterilizing the discharging tube connecting tube together with the discharging portion connecting portion, It is possible to simplify and promptly input.

1 is a cross-sectional view of a microorganism culturing apparatus equipped with an admission unit of the first embodiment according to the present invention.
2 is an enlarged view of part A of Fig.
FIG. 3 is a perspective exploded view of the main part of the feeding section of the first embodiment of the present invention. FIG.
FIG. 4 is a cross-sectional view showing a culture medium introducing portion of the second embodiment according to the present invention. FIG.
FIG. 5 is a perspective exploded view of the main part of the culture medium input part of the second embodiment according to the present invention. FIG.
FIG. 6 is a cross-sectional view of a microorganism culture apparatus equipped with an admission unit of a third embodiment according to the present invention. FIG.
7 is an enlarged view of a portion B in Fig.
FIG. 8 is a perspective view of a main part of a culture medium introducing portion of a third embodiment according to the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood, however, that the invention can be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

FIG. 1 is a cross-sectional view of a microorganism culture apparatus equipped with a culture medium input unit according to a first embodiment of the present invention, FIG. 2 is an enlarged view of part A of FIG. 1, Fig.

As shown in the figure, the microorganism culturing apparatus equipped with the culture medium input unit of the first embodiment according to the present invention comprises a culture tank 1 filled with a mixture (culture liquid) of a culture medium and a seed culture (hereinafter referred to as "microorganism" .

The culture medium input part 2 of the first embodiment according to the present invention is provided on one side of the upper part of the culture tank 1 and serves to feed the culture medium and microorganisms.

The culture medium input unit 2 of the first embodiment according to the present invention has a vertical communication pipe 21 protruded upward from one side of the upper part of the culture tank 1, A vertical connection pipe 23 connected to an upper portion of the first opening and closing valve 22 and a second opening and closing valve 22 mounted on an upper portion of the vertical connection pipe 23, An inlet pipe 25 connected to an upper portion of the second opening and closing valve 24, a closing pipe cap 26 closing an upper portion of the inlet pipe 25, And a steam discharge pipe 28 connected to the outer surface of the vertical connection pipe 23 and capable of opening and closing the steam supply pipe 27.

The vertical communicating tube 21 is vertically formed to protrude upward from the upper plate of the culture tank 1 and is formed to communicate with the culture tank 1. The vertical communicating tube 21 is made of the same material as the culture tank 1 and fixed to the culture tank 1 through welding.

The vertical communication pipe (21) is formed on the outer circumferential surface and includes a threaded portion for connecting the first opening / closing valve (22). The screw portion may be formed only on the upper outer circumferential surface of the vertical communicating pipe 21 so as to be connected to the first opening / closing valve 22.

The first open / close valve 22 is fastened to the screw portion formed on the outer circumferential surface of the vertical communicating tube 21, and a tab portion for fastening the screw portion of the vertical communicating tube 21 is formed in the lower portion of the inner flow path (Not shown) that opens and closes the flow path by operating the knob N exposed to the outside of the inner flow path, and a tap portion to which the vertical connection pipe 23 is fastened is formed on the upper portion of the inner flow path. .

However, since various kinds of the first opening / closing valve 22 are already known, detailed description of the structure and operating relationship thereof will be omitted.

The vertical connection pipe 23 is connected to the upper portion of the first on-off valve 22, and a threaded portion is formed on an outer circumferential surface of the lower connection pipe 22 to be fastened to a tab portion formed on the flow path of the first on- do.

And a screw portion for connecting the second on-off valve 24 is formed on an outer circumferential surface of the upper portion of the vertical connection pipe 23. The vertical connection pipe 23 is preferably made of a metal material or a synthetic resin material so as to maintain durability to withstand the pressure of steam.

The second open / close valve (24) is fastened to the screw portion formed on the outer circumferential surface of the vertical connection pipe (23), and a screw portion formed on the upper portion of the vertical connection pipe (23) And an opening / closing unit for opening and closing the flow path by operating the knob N exposed to the outside is formed at the middle of the internal flow path, (Not shown).

However, since various kinds of the first opening / closing valve 22 are already known, the detailed description of the structure and operating relationship thereof is omitted.

The inlet pipe 25 is connected to the upper portion of the second open / close valve 24, and has a discharging portion 30 of the discharging cylinder 20 filled with a liquid medium to insert the liquid medium .

The injection pipe 25 having such a function is formed with a threaded portion to be fastened to the tab portion formed on the upper portion of the flow path of the first on-off valve 22 on the outer peripheral surface of the lower portion.

The inlet pipe 25 is formed in the upper portion of the inlet pipe 25 and the lower portion of the outlet pipe 30 formed in the lower portion of the inside of the pipe pipe 251, A horizontal close contact surface 252 to which the peripheral end portion is closely contacted and a threaded portion 253 formed on the outer peripheral surface of the expanded portion 251.

The horizontal contact surface 252 is formed in such a manner that when the filled liquid medium of the discharge tube 20 is supplied, the peripheral end portion of the discharge portion 30 of the discharge tube 20 is closely attached, To the inside of the injection pipe (25).

In addition, the inlet pipe cap 26 serves to block the upper portion of the inlet pipe 25 so as to prevent foreign contaminants from penetrating into the inlet pipe 25 at all times. The inlet pipe cap 26 which functions as described above is fastened and fixed to a screw portion 253 formed on the outer peripheral surface of the expanded portion 251.

The inlet tube cap 26 is formed by a circular plate which blocks the upper part of the injection tube 25 and a fastening tube 25 which is formed to protrude downward from the circumference of the circular plate and is fastened to a threaded portion 253 formed on the outer circumferential surface of the tube- . Therefore, the tightening pipe can be rotated to separate or tighten the charging pipe cap 26 from the expansion pipe 251.

The steam supply pipe 27 is connected to the outer surface of the vertical connection pipe 23 to sterilize the inside of the feed input unit 2 by injecting steam from the outside into the feed input unit 2 It plays a role.

The steam supply pipe 27 is connected to the steam generator 10 and is provided with a third open / close valve 271 which can be operated from the outside. The steam generator 10 boils water to generate steam. The steam generator 10 is already known, and a description of the structure and operation of the steam generator 10 will be omitted. The steam supply pipe 27 is preferably made of metal or synthetic resin so as to maintain durability against the pressure of steam.

The third on-off valve 271 serves to interrupt the supply of steam through the operation of the knob N. Since the third on-off valve 271 is known in various types, The description is omitted.

The steam discharge pipe 28 is connected through the outer surface of the vertical connection pipe 23 to discharge the steam filled in the discharge portion 2 to the outside so that the inside of the discharge portion 2 can be promptly discharged Cooling.

The steam discharge pipe 28 has a discharge end portion exposed to the atmosphere, and a fourth open / close valve 281, which can be operated from the outside, is installed in the middle. The fourth on-off valve 281 interrupts the supply of steam through the operation of the knob N. Since various kinds of the fourth on-off valve 281 are known, .

Accordingly, the fourth on-off valve 281 is configured to sterilize the inside of the medium feeding unit 2 by injecting steam into the medium feeding unit 2, and then the fourth on-off valve 281 is immediately opened, And discharges the steam supplied into the unit 2 to the atmosphere, and then closes the fourth opening / closing valve 281, thereby quickly cooling the culture medium input unit 2. [

Hereinafter, a process of feeding a culture medium filled in the culture container through the culture medium input unit according to the first embodiment of the present invention will be described.

As shown in FIGS. 1 to 3, when a liquid medium filled in the discharge tube 20 is introduced through the medium feeding section 2 of the first embodiment according to the present invention, The steam generator 10 connected to the steam supply pipe 27 before the filled medium of the discharge tube 20 is put in a state in which the valves 22 and 24 and the third and fourth opening and closing valves 271 and 281 are closed .

Next, when the third on-off valve 271 is opened and the steam is filled in the discharge inlet portion 2, the third on-off valve 271 is closed. Then, the inside of the vertical connection pipe 23 is sterilized by waiting for the time required for sterilization. In the process of sterilizing the inside of the vertical connection pipe 23, the steam heat is conducted to the first opening / closing valve 22, the second opening / closing valve 24, and the inlet pipe 25, 22, the second open / close valve 24, and the inlet pipe 25 are sterilized together.

When the sterilization of the inside of the vertical connection pipe 23 is completed, the fourth opening / closing valve 281 is opened to discharge the steam filled in the vertical connection pipe 23 into the atmosphere through the steam discharge pipe 28 do. Then, the fourth on-off valve 281 is closed immediately after the discharge of steam is finished.

The discharge portion 30 of the discharge tube 20 is immediately inserted into the expansion portion 251 of the discharge tube 25 after the discharge portion lid 40 of the discharge tube 20 filled with the next liquid medium is opened, The end of the discharge portion 30 of the discharge tube 20 is brought into close contact with the horizontal close contact surface 252 formed in the inner lower portion of the expansion portion 251 while the discharge tube 20 is turned upside down.

And then the first on-off valve 22 and the second on-off valve 24 are opened to feed the liquid culture medium filled in the culture container 20 into the culture tank 1.

When the feeding of the medium filled in the discharge tube 20 is completed through the above process, the first opening / closing valve 22 and the second opening / closing valve 24 are closed again and the discharge tube 20 is connected to the inlet pipe 25 The process of inserting the medium is completed through the process of separating the medium into the medium.

The discharge unit 30 of the discharge tube 20 is connected to the inlet pipe 25 after the steam feed unit 2 is sterilized by only feeding the steam into the steam feed unit 2 and discharging the steam to the outside, And the liquid medium is inserted through the process of inserting the liquid medium into the expanded part 251 of the liquid medium. In addition, as described above, the sterilization structure of the medium feeding section 2 can be simplified, and the production cost of the medium feeding section 2 can be reduced.

Meanwhile, a steam discharge pipe 28 for discharging steam to the atmosphere is installed in the discharge portion 2 to discharge the steam used for sterilizing the discharge portion 2 immediately to the outside so that the discharge portion 2 can be promptly And cooling can be performed, thereby further reducing the feeding time of the culture medium.

FIG. 4 is a cross-sectional view showing a feeding part of the second embodiment according to the present invention, and FIG. 5 is a perspective exploded view of the feeding part of the feeding part of the second embodiment according to the present invention.

As shown in the figure, it is presumed that the medium feeding section 2 of the second embodiment according to the present invention includes all of the medium feeding section 2 of the first embodiment described above.

The culture medium input part 2 of the second embodiment according to the present invention is inserted into the expansion part 251 formed in the upper part of the injection pipe 25 to discharge the discharge part lid 40 And a perforated circular plate 29 for opening the perforated circular plate 29.

The perforated circular plate 29 includes a plurality of vertical through holes 291 formed around the center and communicating with the inside of the injection pipe 25 and a plurality of vertical through holes 291 formed to protrude from the center of the upper surface, 292a and a sharp-pointed perforation needle 292.

Therefore, when a liquid medium filled in the discharge tube 20 is introduced through the medium feeding section 2 of the second embodiment according to the present invention, first, as described in the first embodiment, 2 is sterilized by supplying and discharging steam to the inside of the medium input unit 2.

The surface of the discharging portion lid 40 of the discharging portion 20 is disinfected and the discharging portion 30 of the discharging portion 20 is covered with the discharging portion lid 40 of the discharging portion 20 And the discharge tube 20 is upside down while being inserted into the expansion portion 251 of the injection tube 25. [ The discharging portion lid 40 is inserted into the opening 292 of the perforated circular plate 29 while the discharging portion 30 of the discharging container 20 is inserted into the expanding portion 251, So that a through hole is formed in the discharge port lid 40. The perimeter of the flat portion of the discharging portion lid 40 of the discharge tube 20 is closely attached to the perimeter of the perforated circular plate 29 to prevent penetration of contaminants from the outside.

Next, the first on-off valve 22 and the second on-off valve 24 are opened to feed the liquid medium filled in the culture container 20 into the culture tank 1. Then, when the feeding of the medium filled in the discharge tube 20 is completed, the first opening / closing valve 22 and the second opening / closing valve 24 are closed again, and the discharge tube 20 is separated into the inlet tube 25 To complete the input of the medium.

Therefore, there is an advantage that the culture medium can be simply and promptly put into the culture tank 1 through the process of inverting the culture medium container 20 without opening the discharge port cover 40 of the culture medium container 20.

A tab portion 254 is further formed on the inner circumferential surface of the expanded portion 251 and a threaded portion 293 is formed on an outer circumferential surface of the perforated circular plate 29 to be fastened to the tab portion 254. Therefore, the height of the perforated circular plate 29 is adjusted by rotating the perforated circular plate 29 so that the outlet port of the discharge cylinder 20 can be guided through the perforated circular plate 29 regardless of the height of the outlet port of the discharge cylinder 20, It is also possible to appropriately puncture the pawl 40.

6 is an enlarged view of a portion B of FIG. 6, and FIG. 8 is a cross-sectional view of the microorganism culturing apparatus of FIG. 6 according to the third embodiment of the present invention. It is a lambship perspective.

As shown in the figure, it is presumed that the medium feeding section 2 according to the third embodiment of the present invention includes all of the medium feeding section 2 of the first embodiment except for the feeding tube 25.

Therefore, the inlet pipe 25 of the medium feeding section 2 of the third embodiment according to the present invention is connected to the upper part of the second opening / closing valve 24 and is constructed as an intact pipe. The inlet pipe 25 further includes a flange 255 protruding outward from the upper end of the inlet pipe 25.

In addition, in the third embodiment of the present invention, the connection flange 311 is formed around one end so as to be in close contact with the upper surface of the flange 255 of the inlet pipe 25, A discharge pipe connecting pipe 31 provided with a connection lid 312 for closing the discharge part 30 of the discharge tube 20 and a flange 255 surrounding the flange 255 and the connection flange 311, And a flange clamp (C) connecting the connection flange (311).

A flexible tube made of a metal material or a synthetic resin is provided between the connection flange 311 and the connection lid 312 at both ends so that the flexible tube can be bent.

Since the flange clamp C is wrapped around the connection flange 311 between the flange 255 of the injection pipe 25 and the discharge pipe connection pipe 31 and is known in various types, And a description of the clamping relationship will be omitted.

Therefore, when the liquid medium filled in the discharge tube 20 is introduced through the medium feeding section 2 of the third embodiment according to the present invention as described above, first, the end of the discharge tube connecting tube 31 And the connecting flange 311 formed at the end of the discharging cylinder connecting pipe 31 is superimposed on and brought into close contact with the flange 255 of the charging pipe 25 . The connection flange 311 of the discharge tube connecting pipe 31 and the flange 255 of the inlet pipe 25 are wrapped with a flange clamp C to connect them.

The steam supply pipe 27 and the steam supply pipe 27 are opened before the filled medium of the discharge tube 20 is put in the closed state of the first and second open / close valves 22, 24 and the third and fourth open / close valves 271, And operates the connected steam generator 10.

Then, the third on-off valve 271 is opened and the second on-off valve 24 is opened so as to communicate with the inlet pipe 25 and the discharge pipe connecting pipe 31. Then, Closing valve (271) is closed as soon as it is filled in the inside of the discharge pipe (31). Then, the inside of the culture medium input section 2 and the inside of the culture medium tube 31 are sterilized for the time required for sterilization.

Next, the fourth on-off valve 281 is opened to discharge the steam filled in the discharge inlet 2 and the discharge cylinder connection pipe 31 into the atmosphere through the steam discharge pipe 28. Then, the fourth on-off valve 281 is closed immediately after the discharge of steam is finished.

The liquid culture medium filled in the culture cylinder 20 is put into the culture tank 1 through the process of inverting the culture cylinder 20 with the first open valve 22 being opened.

When the feeding of the medium filled in the discharge tube 20 is completed through the above process, the first opening / closing valve 22 and the second opening / closing valve 24 are closed again, and the flange clamp C is separated So as to complete the feeding of the culture medium through the process of separating the culture tube connecting tube 31 into the inlet tube 25.

Therefore, the discharging portion 30 of the discharging container 20 and the discharging portion connecting portion 2 are connected to each other through the discharging container connecting pipe 31, and the discharging container connecting pipe 31, together with the discharging portion 2, It is possible to insert the culture medium more easily through the process of sterilizing the culture medium after sterilizing the culture medium and sterilizing the culture medium by inverting the culture medium.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It is clear that the present invention can be suitably modified and applied in the same manner. Therefore, the above description does not limit the scope of the present invention, which is defined by the limitations of the following claims.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the present invention.

1: Culture tank
2:
21: Vertical communicator
22: first opening / closing valve
23: vertical connector
24: second opening / closing valve
25: input tube
251:
252: Horizontal close contact surface 253:
254: tab portion 255: flange
26:
27: steam supply pipe
271: Third open / close valve
28: Steam outlet pipe
281: the fourth opening / closing valve
29: Perforated circular plate
291: Vertical hole 292: Perforated needle
292a: discharge recess groove 293: thread portion
31: Discharge tube connector
311: connecting flange 312: connecting lid
C: Flange clamp
N: Knob
10: Steam generator
20: Dispenser
30:
40: outlet cover

Claims (5)

1. A microorganism culturing apparatus comprising a culture tank (1) provided with a culture medium input portion (2) at an upper side,
The culture medium feeding section (2)
A vertical communicating tube 21 formed to protrude upward from the upper plate of the culture tank 1 and having a threaded portion formed on an outer circumferential surface thereof;
A first opening / closing valve (22) fastened to the upper portion of the vertical communicating tube (21);
A vertical connection pipe (23) connected to an upper portion of the first opening / closing valve (22) and having a screw portion formed on an outer circumferential surface of the upper portion;
A second opening / closing valve (24) fastened to the upper portion of the vertical connection pipe (23);
An inlet pipe 25 connected to an upper portion of the second on-off valve 24;
A feed pipe cap 26 for blocking the upper portion of the feed pipe 25;
A steam supply pipe 27 connected to the outer surface of the vertical connection pipe 23 through a third opening / closing valve 271;
A steam discharge pipe 28 connected through the outer surface of the vertical connection pipe 23 and having a fourth opening / closing valve 281;
Wherein the microorganism culturing apparatus has a sterilizing medium input section.
The method according to claim 1,
The inlet pipe 25 is formed in the upper portion of the inlet pipe 25 and the lower portion of the outlet pipe 30 formed in the lower portion of the inside of the pipe pipe 251, And a screw portion 253 formed on an outer peripheral surface of the expanded portion 251,
Wherein the inlet pipe cap (26) is fastened and fixed to the screw portion (253).
3. The method of claim 2,
The culture medium feeding section (2)
A plurality of vertical through holes 291 are formed in the periphery of the center of the inlet tube 25 and a discharge recess 292a is formed in the center of the upper surface of the inlet tube 25 in a direction perpendicular to the periphery thereof, A perforated circular plate (29) in which a perforation needle (292) protrudes;
Wherein the microorganism culturing apparatus further comprises a sterilizing medium input section.
The method of claim 3,
The bulging portion 251 further includes a tab portion 254 formed on an inner peripheral surface thereof,
Wherein the perforated circular plate (29) further comprises a threaded portion (293) formed on an outer circumferential surface of the perforated circular plate (29) and fastened to the tab portion (254).
The method according to claim 1,
The charging pipe 25 further includes a flange 255 protruding outward from the upper end of the charging pipe 25,
The discharge inlet part 2 is formed with a connection flange 311 around one end so as to be in close contact with the upper surface of the flange 255 of the discharge pipe 25, A discharge pipe connecting pipe 31 having a connecting lid 312 for closing the connecting flange 31 and covering the flange 255 and the connecting flange 311, 311) for connecting the microorganism culturing apparatus (1) to the microorganism culturing apparatus.

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