KR101639413B1 - The microbial culture medium equipped with auto supply - Google Patents
The microbial culture medium equipped with auto supply Download PDFInfo
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- KR101639413B1 KR101639413B1 KR1020150049210A KR20150049210A KR101639413B1 KR 101639413 B1 KR101639413 B1 KR 101639413B1 KR 1020150049210 A KR1020150049210 A KR 1020150049210A KR 20150049210 A KR20150049210 A KR 20150049210A KR 101639413 B1 KR101639413 B1 KR 101639413B1
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- microorganism
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- pipe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
Description
The present invention relates to a microorganism incubator having an automatic microorganism feeder, and more particularly, to a microorganism incubator having an automatic microorganism feeder capable of supplying a microorganism to be cultivated easily and easily into a culture chamber through the microorganism automatic feeder, And more particularly, to a microorganism incubator having an automatic microbial supply unit capable of cleaning the automatic microbial supply unit in a short period of time in a cleaner and easier manner and shortening the subsequent incubation time.
In recent years, technologies for utilizing microorganisms in industry have been widely developed. As a method of using these microorganisms, microorganisms are cultured and sprayed on the soil to promote the production of crops, to prevent pests and diseases, to promote the growth of livestock, And contributes to obtaining high quality meat quality.
In the aquaculture industry, it can also be used for water purification, organic waste fermentation, and industrial waste purification. These microorganisms are also effective in reducing odor and pollution.
Therefore, in order to easily and stably utilize microorganisms, a microorganism culture apparatus is conventionally used.
The conventional microorganism culturing apparatus is configured to be capable of stirring and air injection as well as feeding of the medium.
A conventional conventional microorganism culture apparatus includes a culture tank in which a mixture (culture liquid) of a culture medium and a seed culture (hereinafter, referred to as "microorganism") is stored, a culture tank disposed in the culture tank, A motor for transmitting power by the stirring means, and an air supply unit installed on a bottom surface of the culture tank.
At the upper part of the culture tank, an inoculation port is formed for inoculating the culture medium and seed culture.
The culture medium inlet is composed of a charging tube having a thread formed on the outer peripheral surface thereof and a cap which is fastened to the charging tube and blocks the upper part of the charging tube.
Conventionally, when the culture medium is introduced into the culture tank through the culture medium inlet, the culture medium is flame-treated through the ignition of alcohol around the inlet of the injection tube so as to block contaminants in the air, Is used.
However, when the culture medium is flame-treated in the flame-treated surroundings as described above, there is a risk of burns, contamination of the periphery of the injection tube, contamination of microorganisms in the culture tank, I had a problem.
In order to solve such a problem, Korean Patent Registration No. 0767569 "incubator" has been proposed in the past.
However, such an incubator requires a lot of time for inoculation because the inoculation port of the seedling must be connected to the inoculation line, the sterilization supply line, and the steam supply line of the seedling inoculation line, and the structure of the medium feeding part is complicated, .
It is an object of the present invention to provide a microorganism incubator having an automatic microorganism feeder capable of feeding microorganisms for culturing to a culture chamber prepared without the risk of pollution and safety accidents.
Another object of the present invention is to provide a microorganism incubator having an automatic microbial supply unit capable of cleaning the automatic microbial supply unit after the microbial supply and before culturing another microbial cell in a clean and simple manner in a short period of time, thereby shortening the next incubation time.
In the meantime, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.
A microorganism incubator having an automatic microbial supply unit according to the present invention includes a culture chamber for supplying a microorganism to the culture medium injected with a culture medium to culture the microbe, And a microorganism automatic supply unit for automatically supplying the microorganisms to the inside of the chamber, wherein the microorganism automatic supply unit comprises a storage container for storing the microorganisms mixed in the culture liquid, A microorganism inlet port for injecting the microorganisms into the storage container, a microorganism level checking means for checking the amount of the microorganisms injected into the storage container, a microorganism supply pipe for supplying the microorganisms stored in the storage container to the culture chamber, And a microorganism supply pipe A may include a first switching valve for supplying the microorganisms with the culture chamber.
Preferably, the automatic microorganism supply unit may be connected to the interior of the storage container and the washing unit for washing and sterilizing the microorganism supply pipe.
Preferably, the cleaning part includes a washing pipe provided on the upper part of the storage container, a steam generator for injecting high temperature and high pressure steam into the storage container through the washing pipe, A spray nozzle provided at one end of the washing tube for spraying the steam and the washing water to the inner circumferential surface of the storage vessel, a steam nozzle provided at the steam generator and the water dispenser, A second opening / closing valve provided in the steam generator for controlling whether the steam is injected or not, and a second opening / closing valve provided in the water supply device for supplying water to the steam generator, A third open / close valve for controlling the microorganisms, a third open / close valve connected to the microorganism supply pipe, May include a fourth switching valve for controlling the opening if the water pipe and the drain pipe for discharging the steam and the wash water.
Preferably, the culture chamber may be provided with a temperature sensor for measuring the internal temperature of the culture chamber, and the storage container may be made of a transparent material so that the amount of the stored microorganism can be visually confirmed.
The present invention has the following excellent effects.
First, the microorganism for culturing can be supplied to the culture chamber prepared with the culture liquid without risk of contamination and safety accidents.
In addition, there is an excellent effect that the automatic microorganism supply portion can be cleaned and cleaned more quickly and quickly after the microorganisms are supplied and before the other microorganisms are cultured, so that the subsequent culture time can be shortened.
1 is a perspective view showing the entire structure of a microorganism incubator having an automatic microorganism feeder according to an embodiment of the present invention.
Fig. 2 is a perspective view showing the entire configuration of the culture chamber shown in Fig. 1; Fig.
FIG. 3 is an enlarged view of the main part of the automatic microorganism supply unit shown in FIG. 1, and FIG. 4 is an enlarged view of the main part shown in FIG.
The term used in the present invention is a general term that is widely used at present. However, in some cases, there is a term selected arbitrarily by the applicant. In this case, the term used in the present invention It is necessary to understand the meaning.
Hereinafter, the technical structure of the present invention will be described in detail with reference to preferred embodiments shown in the accompanying drawings.
FIG. 1 is a perspective view showing the entire structure of a microorganism incubator having an automatic microorganism feeder according to an embodiment of the present invention, FIG. 2 is a perspective view showing the entire configuration of the culture chamber shown in FIG. 1, 3 is an enlarged view of the main part of the automatic microorganism supply unit shown in Fig. 1, and Fig. 4 is an enlarged view of the main part shown in Fig.
1 to 4, a
Hereinafter, the
First, the
In this case, the
A plurality of
Meanwhile, the
In addition, a
The reason why the
The reason why the
The
On the other hand, a
On the other hand, the outer surface of the
At this time, the
A
As a result, the
Meanwhile, the
Hereinafter, the automatic
First, the automatic
In this case, the
The upper portion of the
At this time, the water level confirmation means 123 may use various means such as various water pressure sensors or scales on which the capacity is indicated.
In addition, a
At this time, the
The
The
First, the
At this time, as shown in FIG. 4, the
The
The steam generator 1320 has a function of washing and sterilizing the
The steam generated in the
The reason why the steam is passed through the
On the other hand, a second opening /
The second on-off
The
4, the
As shown in FIG. 4, the
On the other hand, the
The third on-off
1, the
In order to prevent the contaminated water from flowing into the
1, a
At this time, the
As a result, the
First, a mixed solution of a microorganism and a culture medium to be cultured is injected into the
At this time, the culture medium for culturing the microorganisms is filled in the
When the inside of the
When the microorganism supply is completed, the first on-off
As a result, the microorganism incubator having the microorganism automatic feeder according to an embodiment of the present invention can supply the microorganisms for culturing through the above-described technical configurations to the culture chamber prepared without the risk of contamination and safety accident, The microorganism automatic feeder can be cleaned and cleaned in a short period of time before the cultivation of another microorganism, so that the subsequent culture time can be shortened.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications may be made by those skilled in the art.
100: Microorganism culturing with automatic supply of microorganisms
110: culture chamber
110a:
111: Temperature sensor
112:
113: blade
114: drive motor
115: Sword eyeball
116:
117: Culture medium inlet
118: Light source device
119: Stand
120: Automatic microorganism supply unit
121: Storage container
122: Microorganism inlet
123: Water level checking means
124: microorganism supply pipe
125: first opening / closing valve
130: The three governments
131: Cleaning pipe
132: Steam generator
133: Watering machine
134: Spray nozzle
135: Filter
136: second opening / closing valve
137: Third open / close valve
138: Water pipe
139: fourth opening / closing valve
140: Check valve
Claims (5)
A culture chamber 110 for supplying a culture solution into the inside of the incubator, which includes a heating unit 116 and a temperature sensor 111 and is maintained at a proper temperature while being heated, for culturing the microorganisms therein by supplying microorganisms to the culture solution; And an automatic microorganism supply unit (120) for automatically supplying the microorganisms into the culture chamber (110) into which the culture liquid is injected,
The automatic microorganism supply unit 120 includes a storage container 121 for storing the microorganisms mixed in the culture liquid; A microorganism inlet 122 provided at an upper portion of the storage container 121 to inject the microorganisms into the storage container 121; A microorganism supply pipe (124) for supplying the microorganisms stored in the storage container (121) to the culture chamber (110); A first opening and closing valve 125 provided in the microorganism feed pipe 124 for automatically feeding the microorganism into the incubation chamber 110 and a second open and close valve 125 for washing the inside of the storage container 121 and the microorganism feed pipe 124 And a drain pipe 138 is connected to the microorganism supply pipe 124 for supplying steam or washing water for sterilizing and discharging contaminated water that has washed the storage container 121 and the microorganism supply pipe 124, ) Comprises a cleaning part (130) which is opened or closed by a fourth opening / closing valve (139)
The inside of the culture chamber 110 filled with the culture medium in the state where microorganisms are injected into the storage container 121 of the automatic microorganism supply unit 120 is preheated to a temperature suitable for culturing microorganisms by the heating unit 116,
The automatic microorganism supply unit 120 opens the first on-off valve 125 when the culture chamber 110 is preheated to a proper temperature and is sensed by the temperature sensor 111, The microorganism is automatically supplied to the culture chamber 110,
When the supply of the microorganisms from the storage container 121 to the culture chamber 110 is completed, the first opening and closing valve 125 is closed and the steam or the washing water is supplied to the storage container 121 and the microorganism supply pipe 124 are cleaned and the polluted water generated by the cleaning is discharged through the drain pipe 138 by opening the fourth opening and closing valve 139. [ Microbiological incubator.
The automatic microorganism supply unit 120 further includes a microorganism level checking unit 123 provided in the storage vessel 121 for checking the amount of the microorganisms to be injected. Incubator.
The cleaner 130:
A washing pipe 131 provided at an upper portion of the storage container 121;
A steam generator 132 for injecting high-temperature and high-pressure steam into the storage vessel 121 through the washing pipe 131;
A water dispenser 133 connected to the washing pipe 131 and supplying washing water to the inside of the storage container 121;
A spray nozzle 134 provided at one end of the wash tube 131 for spraying the steam and wash water onto the inner circumferential surface of the storage vessel 121;
A filter 135 installed in the steam generator 132 and the water dispenser 133 to prevent contaminants present in the steam and the wash water from flowing into the interior of the storage vessel 121;
A second on-off valve (136) provided on the steam generator (132) for controlling whether the steam is injected or not; And
And a third on-off valve (137) provided on the water supply unit (133) for controlling whether the wash water is supplied or not.
Wherein the storage vessel (121) is made of a transparent material so that the amount of the stored microorganism can be visually confirmed.
Priority Applications (1)
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KR1020150049210A KR101639413B1 (en) | 2015-04-07 | 2015-04-07 | The microbial culture medium equipped with auto supply |
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KR1020150049210A KR101639413B1 (en) | 2015-04-07 | 2015-04-07 | The microbial culture medium equipped with auto supply |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101801886B1 (en) | 2017-05-23 | 2017-11-27 | 김경숙 | Automatic system for supplying the effective microorganism |
KR101885118B1 (en) | 2018-05-09 | 2018-09-10 | 주식회사 이룸 | Compact type apparatus for cultivating concentrated microorganism for improving quality of seawater of farm and cultivation method of concentrated microorganism using the same |
KR102002469B1 (en) * | 2018-06-21 | 2019-07-23 | 주식회사 이시스 | Apparatus for cultivating microbe using non-heating means |
KR102002594B1 (en) * | 2018-05-08 | 2019-10-01 | 주식회사 조인트리 | Cleaning apparatus for water tank |
CN110423674A (en) * | 2019-08-19 | 2019-11-08 | 深圳市新舟生物科技有限公司 | A kind of numerous conveying device of microorganism precipitation expansion |
KR20230021922A (en) * | 2021-08-06 | 2023-02-14 | (주)에코비즈넷 | An apparatus for inserting microorganisms |
Citations (2)
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KR20090096662A (en) * | 2009-07-15 | 2009-09-14 | 수성건설 주식회사 | Aparutus for cultivating microorganism |
KR101176408B1 (en) * | 2011-11-22 | 2012-08-28 | 주식회사 쉴드텍 | Compacted culture apparatus |
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2015
- 2015-04-07 KR KR1020150049210A patent/KR101639413B1/en active IP Right Grant
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20090096662A (en) * | 2009-07-15 | 2009-09-14 | 수성건설 주식회사 | Aparutus for cultivating microorganism |
KR101061424B1 (en) * | 2009-07-15 | 2011-09-01 | 수성건설 주식회사 | Microbial Culture Device |
KR101176408B1 (en) * | 2011-11-22 | 2012-08-28 | 주식회사 쉴드텍 | Compacted culture apparatus |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101801886B1 (en) | 2017-05-23 | 2017-11-27 | 김경숙 | Automatic system for supplying the effective microorganism |
KR102002594B1 (en) * | 2018-05-08 | 2019-10-01 | 주식회사 조인트리 | Cleaning apparatus for water tank |
KR101885118B1 (en) | 2018-05-09 | 2018-09-10 | 주식회사 이룸 | Compact type apparatus for cultivating concentrated microorganism for improving quality of seawater of farm and cultivation method of concentrated microorganism using the same |
KR102002469B1 (en) * | 2018-06-21 | 2019-07-23 | 주식회사 이시스 | Apparatus for cultivating microbe using non-heating means |
CN110423674A (en) * | 2019-08-19 | 2019-11-08 | 深圳市新舟生物科技有限公司 | A kind of numerous conveying device of microorganism precipitation expansion |
CN110423674B (en) * | 2019-08-19 | 2024-06-04 | 广东花猫农业科技有限公司 | Microorganism precipitation and propagation conveying device |
KR20230021922A (en) * | 2021-08-06 | 2023-02-14 | (주)에코비즈넷 | An apparatus for inserting microorganisms |
KR102596474B1 (en) * | 2021-08-06 | 2023-10-30 | (주)에코비즈넷 | An apparatus for inserting microorganisms |
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