JP2012029691A - Lid component for container, container equipped with the lid component, and container set - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an easily closable or openable container lid component, a container equipped with the lid component and a set of the container.SOLUTION: The lid component 200 of the culture vessel 500 comprises a body 300 and a lid part 400. The lid part 400 makes a contact surface contact with the opening part 320 of the body 300 with a first hinge part 420, a second hinge part 430 and an engaging part 460 to close the opening part 320 of the body 300. The lid part 400 has a tongue part 470 installed as to project from the contact surface.

Description

  The present invention relates to an instrument for performing experiments, clinical examinations, and tissue cultures, and relates to a container lid member, a container including the lid member, and a container set.

  Conventionally, experiments and research have been carried out by human work in culture researches such as cell culture, especially immune cells. As a result, a culture vessel that allows easy experimentation and research is required, and various developments have been made on the culture vessel.

  Patent Document 1 discloses an improved assembly of a culture container and a closing member that can be taken out from an incubator for a long time.

  The assembly of the culture vessel and the closing member described in Patent Document 1 is an assembly that is used for growing a cell culture in an incubator, and includes a chamber and a chamber coupled to the chamber. A container including a neck portion having an opening for introducing cells and culture medium therein, and a closing member for covering the opening provided in the neck portion, wherein the closing member can be freely removed from the neck portion An occluding member including means for attaching and means for allowing diffusion of gas into and out of the container and the occluding member to prevent diffusion of gas into and out of the container And a plug detachably attached to the housing.

Japanese Patent Laid-Open No. 07-163333

  In recent years, automation is also required in the culture field due to rapid progress in cell engineering or medical engineering. The automation has a merit that large-scale culture can be performed in an aseptic environment, and is attracting attention as a technique for promoting a disease treatment method.

  However, in the assembly of the culture container and the closing member described in Patent Document 1, although it is possible to perform an operation of manually pulling out the plug from the cap or an operation of inserting the plug into the cap, The operation of removing the plug from the cap and the operation of inserting the plug into the cap are difficult. That is, problems such as positioning of the plug occur, automation becomes complicated, and costs increase.

  Further, not only in automation, but also in the case of human work, since the plug comes off the cap and becomes an independent member, problems such as loss of the plug occur.

An object of the present invention is to provide a container lid member that can easily close or open the container, a container including the lid member, and a container set.
Another object of the present invention is to provide a cover member for a sterile environment container that can be easily closed or opened, a sterile environment container including the cover member, and a sterile environment container set. .

(1)
A lid member for a container according to one aspect is a lid member for a container, and is fitted into the container and engaged with the main body by the connecting portion, and the opening is closed and opened. A lid portion that includes a locking portion that locks the lid portion to the main body portion, and a protruding portion that protrudes from a contact surface that contacts the main body portion.

  The lid member of the container includes a main body portion and a lid portion. The lid portion is brought into contact with the main body portion by the connecting portion and the locking portion, and closes the opening portion of the main body portion. Further, the lid portion has a protruding portion provided protruding from the contact surface.

  In this case, the lid member includes a main body portion and a lid portion. By opening only the lid part with the main body part fitted to the container, the opening part of the main body part is opened, and the internal space and the external space of the container can be easily communicated. Furthermore, since the protruding portion is provided so as to protrude from the contact surface that contacts the main body portion, the locking of the locking portion can be easily released by applying a force to the protruding portion.

(2)
In the lid member of the container, the lid portion may have a hole portion, and the filter member may be disposed in the hole portion.

  In this case, since the hole is provided in the lid and the filter member is provided in the hole, even when the lid is locked to the main body, only clean air is provided between the internal space and the external space of the container. Can be passed. In addition, when the container is a culture container, when exchanging the culture solution in the culture container, the internal space can be removed without releasing the main body by releasing the locking of the lid and the main body and exposing the opening. The culture medium can be exchanged. Moreover, the filter member can be reliably held by providing the filter member on the contact surface side. On the other hand, the filter member can be easily replaced by providing the filter member on the surface side facing the contact surface side.

Further, in the case of a container such as a centrifuge tube, gas can enter and exit through a filter member when sterilization is performed with high-pressure steam generally called an autoclave.
Specifically, when high-pressure steam sterilization of a container such as a centrifuge tube in which the waste liquid has been stored, the internal pressure of the centrifuge tube increases and the container breaks, or high-pressure steam does not enter inside. Since there is a problem, in general, high-pressure steam sterilization is performed after the user performs an act of removing or loosening the lid.

Therefore, there is a risk of contamination in both the act of removing the lid by the user and the act of loosening the lid.
On the other hand, in the lid member of the present invention, the hole portion is provided in the lid portion and the filter member is provided in the hole portion. Therefore, even when the lid portion is locked to the main body portion, the internal space of the container and the outside High-pressure steam can be passed between the space and the danger of user contamination can be prevented.

(3)
In the lid member of the container, the protruding portion may be provided on at least an extension in the direction from the coupling portion to the locking portion.

  In this case, since the protruding portion is provided on the extension in the direction from the connecting portion to the locking portion, applying a force to the protruding portion applies a large force to the locking portion with the connecting portion as the rotation axis. And the locking portion can be easily removed.

(4)
In the lid member of the container, the protruding portion may have a plate shape.

  In this case, since the protruding portion has a plate shape, the locking portion of the lid member can be easily released by the automatic opening device in a state where cells or the like are cultured in the culture container. As a result, the culture medium in the inner space of the culture vessel can be automatically replaced by opening the opening. That is, a large amount of culture containers can be processed in a short time.

(5)
In the lid member of the container, the protruding portion may have a tongue shape.

  In this case, since the protruding portion has a tongue shape, the locking portion of the lid member can be easily released by the automatic opening device in a state where cells or the like are cultured in the container. As a result, the culture medium in the inner space of the container can be automatically replaced by opening the opening. That is, a large amount of culture containers can be processed in a short time.

(6)
In the lid member of the container, the main body portion and the lid portion may be provided integrally.

  In this case, since the main body portion and the lid portion are provided integrally, it is possible to prevent the lid portion from being lost. Moreover, cost reduction can be aimed at by forming integrally.

(7)
In the container lid member, the container may be a culture container or a centrifuge tube, and the opening of the main body may have a dripping prevention part.

  In this case, since the dripping prevention part is provided in the opening part of the container, dripping does not occur around the opening part when the culture solution or the like is discharged from the culture container or the centrifuge tube.

(8)
In the lid member of the container, the dripping prevention part may comprise a standing wall extending from the periphery of the opening of the main body part toward the lid part.

  In this case, since the dripping prevention part is composed of a standing wall extending from the periphery of the opening part to the lid part side, no dripping occurs around the opening part when the culture solution or the like is discharged. In particular, dripping can be prevented by making the tip of the standing wall thinner so that the surface tension of the culture solution does not occur.

(9)
In the lid member of the container, the dripping prevention portion may be provided in a range of about 180 degrees around the circumference of the opening at a position away from the connecting portion around the opening of the main body.

  In this case, the dripping prevention part is provided in a range of approximately 180 degrees on the circumference of the opening at a position away from the coupling part around the opening of the main body. When the drainage processing of the culture solution or the like is performed from the opening, the connecting portion is arranged at least vertically upward from the center of the opening, so that it passes through the dripping prevention portion from the opening of the lid member for a short time. The culture medium and the like can be discharged.

(10)
When the lid is a lid member of the container and the opening is closed, the lid may have a recess that sandwiches the standing wall.

  In this case, when the lid portion closes the opening portion, the lid portion has a recess for sandwiching the standing wall, so that the standing wall can be formed large. As a result, dripping can be prevented.

(11)
A container set according to another aspect is a container set, and includes the lid member according to any one of claims 1 to 6 and a container.

  In this case, the lid member includes a main body portion and a lid portion. By opening only the lid part with the main body part fitted to the container, the opening part of the main body part is opened, and the internal space and the external space of the container can be easily communicated. Furthermore, since the protruding portion is provided so as to protrude from the contact surface that contacts the main body portion, the locking of the locking portion can be easily released by applying a force to the protruding portion.

(12)
When the lid member is attached to the container of the container set, the container has a placement surface for placing on the table, and the connection portion of the lid member is placed on the basis of the center of the opening. It may be arranged on the opposite side.

  In this case, since the connecting portion is disposed at a position away from the center of the opening from the placement surface (vertically upward from the center), when the lid member is opened, the lid portion is placed on the placement surface side. Not arranged. As a result, when exchanging the culture solution in the container from the opening, even if there are miscellaneous bacteria in the exchange equipment (part that does not contact the culture solution of the culture medium exchange nozzle), even if the miscellaneous bacteria falls down, The contact surface side of the lid does not serve as a tray. That is, since the contact surface on the opening side that communicates with the inside of the container does not serve as a tray, contamination of germs and the like into the container can be reduced to the limit. In addition, the said mounting surface means the site | part which can be mounted in a plane or an exclusive holder, etc., and includes arbitrary planes, a curved surface, a support leg, etc.

(13)
In the container set, it is preferable that the dripping prevention part is provided in a range of approximately 180 degrees around the circumference of the opening including at least the placement surface side.

  In this case, since the dripping prevention part is provided in a range of about 180 degrees around the circumference of the opening including at least the placement surface side, the side opposite to the opening side of the placement surface is raised vertically upward, By inclining the container, it is possible to discharge the culture solution and the like in a short time.

(14)
In the container lid member, the container may be a culture container or a centrifuge tube.

  In this case, the inner space and the outer space of the container can be easily communicated with each other in a culture container or a centrifuge tube used in a sterile environment.

(15)
In the container lid member, the container may be a flask or a roller bottle.

  In this case, the container may be a flask or a roller bottle. That is, cell culture can be processed in large quantities by using mass-produced flasks and roller bottles. The container may be another container such as a rotary flask or a stirring bioreactor.

(16)
A container according to another aspect includes a lid portion that is integrally formed with the cylindrical mouth portion of the container and has a connecting portion, and the lid portion includes a locking portion that latches the lid portion to the cylindrical mouth portion of the container, And a projecting portion that projects from the abutting surface that abuts.

  In this case, the lid is integrally formed on the container. By opening only the lid, the tube opening of the container is opened, and the internal space and external space of the container can be easily communicated. Furthermore, since the protruding portion is provided so as to protrude from the contact surface that contacts the tube opening, the locking of the locking portion can be easily released by applying a force to the protruding portion.

(17)
In the container, the container may be a culture container or a centrifuge tube, and the tube opening may have a dripping prevention part.

  In this case, since the dripping prevention part is provided in the tube opening part of the container, dripping does not occur around the tube opening part when the culture solution or the like is discharged from the culture container or the centrifuge tube.

(18)
In the container, the container may have a mounting surface for mounting on the table, and the connecting portion of the lid member may be disposed on the opposite side of the mounting surface with respect to the center of the tube opening.

  In this case, since the connecting portion is disposed at a position away from the center of the tube opening portion (vertically upward from the center) from the placement surface, when the lid member is opened, the lid portion is disposed on the placement surface side. Not arranged. As a result, when exchanging the culture solution in the container from the tube opening, there are miscellaneous bacteria in the exchange equipment (part that does not contact the culture solution of the culture solution exchange nozzle), even if the miscellaneous bacteria fall downward, The contact surface side of the lid does not serve as a tray. That is, since the contact surface on the tube opening side communicating with the inside of the container does not serve as a tray, contamination of germs and the like into the container can be reduced to the utmost limit. In addition, the said mounting surface means the site | part which can be mounted in a plane or an exclusive holder, etc., and includes arbitrary planes, a curved surface, a support leg, etc.

  According to the container lid member, the container including the lid member, and the container set according to the present invention, the container can be easily closed or opened.

It is a typical external view which shows an example of the state which fitted the cover member to the culture container which concerns on this invention. It is a typical top view of a lid member. It is a typical side view of a lid member. It is typical explanatory drawing explaining the AA line cross section of a cover member. FIG. 5 is a schematic explanatory diagram illustrating a closed state of the lid member of FIG. 4. It is typical sectional drawing which shows an example of operation | movement of a cover member. It is typical sectional drawing which shows an example of operation | movement of a cover member. It is typical sectional drawing which shows an example of operation | movement of a cover member. It is a typical sectional view showing other examples of a lid member. It is a typical sectional view showing other examples of a lid member. It is a schematic diagram explaining the effect of a cover member. It is a typical perspective view which shows the other example of a dripping prevention rib. It is a typical sectional view showing other examples of a lid member. It is a schematic plan view which shows the other example of a cover member. It is a typical side view showing other examples of a lid member. It is a typical external view which shows the other example of a culture container set.

Hereinafter, embodiments of the present invention will be described with reference to the drawings. In the following embodiments, a container (culture container) used in a sterile environment is illustrated as a container. The present invention is not limited to a culture container, but may be any other container such as a centrifuge tube that is used in another sterile environment, a container set that is used in a sterile environment, and a container other than a sterile environment. Can also be applied.
(First embodiment)

  FIG. 1 is a schematic external view showing an example of a state in which a lid member is fitted to the culture vessel according to the present invention, FIG. 2 is a schematic plan view of the lid member 200, and FIG. 3 is a schematic view of the lid member 200. FIG. 4 is a schematic explanatory view for explaining a cross section of the lid member 200 along the line AA, and FIG. 5 is a schematic explanation for explaining a closed state in which the lid member 200 of FIG. 4 is rotated in the direction of the arrow R1. FIG.

  As shown in FIG. 1, the culture vessel set 100 according to the present embodiment includes a culture vessel 500 and a lid member 200. The culture vessel 500 is a culture flask. The culture vessel 500 is used with the mounting surface 530 as the lower surface during culture. The culture vessel 500 is provided with a cylindrical portion 520 on one surface of a substantially cubic shape in which a space is formed, and the cylindrical outlet 510 causes the internal space of the culture vessel 500 and the internal space of the cylindrical portion 520 to be It has a connected structure. A spiral male screw is formed on the outer peripheral surface of the end of the cylindrical portion 520 of the culture vessel 500.

  As shown in FIGS. 2 to 5, the lid member 200 mainly includes a main body 300, a lid 400, and a filter 550. In the present embodiment, main body 300 and lid 400 are integrally formed.

  The main body 300 mainly includes a housing 310, and an opening 320, a locking rib 330, and a female screw 340 (see FIG. 4) are formed in the housing 310.

  The lid portion 400 mainly includes a disc lid 410, and a first hinge portion 420, a second hinge portion 430, a hole portion 440, a sealing rib 450, a locking portion 460 and a tongue portion 470 are formed on the disc lid 410. Is done.

Details will be described below. The housing 310 of the main body 300 has a substantially cylindrical shape with an opening 320 provided at the center, and an internal thread 340 formed of a spiral groove is formed on the inner periphery. The female screw 340 is fitted with a male screw formed on the peripheral surface of the end portion of the cylindrical portion 520 of the culture vessel 500. As a result, the culture vessel 500 and the lid member 200 are fastened as shown in FIG.
A locking rib 330 is formed on a part of the outer peripheral portion of the housing 310 of the main body 300.

On the other hand, the lid 400 includes a disc lid 410, and the disc lid 410 is formed so as to be connected to the housing 310 of the main body 300 by the first hinge portion 420 and the second hinge portion 430.
In the present embodiment, two first hinge portions 420 are formed, and one second hinge portion 430 is formed. The first hinge part 420 and the second hinge part 430 are formed so that the thicknesses of the hinges are different from each other.

  In addition, a hole 440 is formed in the center of the disc cover 410, and a sealing rib 450 is formed around the hole 440. The sealing rib 450 is formed so that it can be liquid-tightly fitted along the inner peripheral surface of the opening 320 of the main body 300. A filter 550 is provided inside the sealing rib 450.

  The filter 550 is for performing gas exchange between the internal space of the culture vessel 500 and the external environment of the culture vessel 500 so that cells to be cultured do not suffocate, and includes airborne bacteria present in the external environment. Prevents invasion of fungi or spores. In general, a nonwoven fabric filter or a membrane filter having an opening diameter of 0.45 μm or less, preferably 0.20 μm or less is used.

  A locking portion 460 is provided on the circumferential portion of the disc lid 410. The locking portion 460 is provided to lock with the locking rib 330 of the housing 310 (see FIG. 5). Here, the locking portion 460 has a protrusion structure that is provided with a protrusion on the body portion 300 side of the tongue portion 470 and that is locked or fitted to the locking rib 330 of the body portion 300. Further, the disc cover 410 is provided with a tongue portion 470. As shown in FIG. 1, the tongue 470 is formed so as to face downward when it is attached to a culture vessel 500 having a mounting surface 530 on the bottom surface.

That is, when the lid member 200 is fastened to the culture vessel 500, the female screw of the lid member 200 is provided with respect to the male screw 340 of the culture vessel 500 so that the tongue portion 470 faces downward.
Specifically, when the lid member 200 is fastened to the culture vessel 500 with a predetermined tightening torque, the male screw 340 and the lid member are arranged so that the lid member 200 is completely fastened to the culture vessel 500 when the tongue portion 470 faces downward. The internal threading positions of the 200 female threads are provided to match. Note that only the tightening torque may be controlled so that the tongue 470 faces downward.

  As a result, by turning the tongue 470 downward, when the disc lid 410 is opened and closed, the disc lid 410 exists as a foreign matter-preventing roof above the opening 320, and live bacteria fall into the opening 320. Mixing can be prevented. That is, the first hinge part 420 and the second hinge part 430 are disposed on the opposite side of the placement surface 530 of the culture vessel 500 through the center of the opening 320. In the present embodiment, the first hinge part 420 and the second hinge part 430 are disposed on the opposite side of the mounting surface 530 of the culture vessel 500 through the center of the opening 320. However, the present invention is not limited to this, and it is only necessary to be disposed on the opposite side of the placement surface 530 of the culture vessel 500 with the center of the opening 320 as a reference.

  Next, a case where the lid of the lid member 200 is opened and closed by automation will be described. 6 to 8 are schematic cross-sectional views showing an example of the operation of the lid member 200. FIG.

  First, as shown in FIG. 6, when the lid member 200 is in a closed state, the internal space of the culture vessel 500 and the external space of the culture vessel 500 communicate with each other through the opening 320, the hole 440 and the filter 550. Yes. That is, only clean air can be passed through the internal space by the filter 550.

  Next, as shown in FIGS. 6 and 7, the pin 610 of the automatic machine pushes the tongue 470 in the direction of the arrow R2. As a result, a force is applied to the tongue portion 470 around the first hinge portion 420 and the second hinge portion 430, and the locking portion 460 of the disc cover 410 releases the locking with the locking rib 330.

  Next, as shown in FIG. 8, the pin 610 of the automatic machine moves the tongue 470 in the direction of the arrow R3. Thereby, the disc cover 410 moves upward, and the cover member 200 is opened. As a result, the opening 320 allows communication between the internal space of the culture vessel 500 and the external space of the culture vessel 500. Then, the culture medium exchange nozzle 620 passes through the opening 320 by the automatic machine, the culture medium in the culture vessel 500 is sucked, and a new culture medium is supplied.

  Thereafter, the culture medium exchange nozzle 620 is pulled out from the opening 320, and the pin 610 of the automatic machine pushes the tongue 470 (the surface opposite to the surface in the figure) in the order of FIGS. The lid member 200 can be closed.

(Second Embodiment)
Next, FIGS. 9 and 10 are schematic cross-sectional views showing other examples of the lid member 200, FIG. 11 is a schematic view for explaining the effect of the lid member 200a, and FIG. It is a typical perspective view which shows another example.

  As shown in FIGS. 9 and 10, the main body 300 of the lid member 200 a according to the second embodiment mainly includes a housing 310, and the housing 310 has an opening 320, a locking rib 330, an internal thread. 340, holding rib 350, and dripping prevention rib 360 are formed.

9 is provided to hold the vicinity of the cylindrical portion 520 of the culture vessel 500, and the dripping prevention rib 360 is provided around the opening 320.
The dripping prevention rib 360 has a height L and a width H. The height H is preferably in the range of about 3 mm to about 6 mm. The width H is preferably large enough not to cause surface tension of the culture solution. For example, in the case of the culture solution, the width H is preferably about 3 mm or less.

  The lid portion 400 mainly includes a disc lid 410, and a first hinge portion 420, a second hinge portion 430, a hole portion 440, a sealing rib 450, a concave portion 451, a locking portion 460, and a tongue portion 470 are formed.

  As shown in FIG. 10, when the lid 400 is rotated in the direction of the arrow R <b> 1 with respect to the main body 300 to be in the closed state, the recess 451 is provided so as to double-seal the dripping prevention rib 360. Here, the double seal refers to a state where the liquid dripping prevention ribs 360 are in contact with each other.

  Moreover, as shown in FIG. 11, in the lid member 200a according to the second embodiment, the culture medium can be discharged in the direction of the arrow FL by inclining the mounting surface 530 by an automatic machine. . Therefore, it is possible to perform disposal processing for discharging the culture solution in a short time, and automation can be achieved. Further, since the lid member 200a according to the second embodiment has the dripping prevention rib 360, dripping does not occur.

  Furthermore, as shown in FIG. 12 (a), a dripping prevention rib 360 may be provided around 360 degrees around the opening 320, and as shown in FIG. 12 (b), the opening including the mounting surface 530 is provided. A dripping prevention rib 360h may be provided around the portion 320 around a half circumference of 180 degrees.

(Third embodiment)
Next, FIG. 13 is a schematic cross-sectional view showing another example of the lid member 200.

  As shown in FIG. 13, the lid member 200 b mainly includes a disc lid 410, and a first hinge part 420, a second hinge part 430, a sealing rib 450, a locking part 460 and a tongue part 470 are formed. That is, this is a type in which the hole 440 of the lid member 200 shown in FIG. 2 is not formed. Also, the filter 550 is not provided.

  In this case, it is effective for cells and the like that can be cultured only with the air in the culture vessel 500 and the culture solution. That is, it is not necessary to exchange air and culture medium.

(Fourth embodiment)
Next, FIG. 14 is a schematic plan view showing another example of the lid member 200.

  As shown in FIG. 14, the lid member 200 c is different from the lid members 200, 200 a, and 200 b, and the shape of the tongue portion 470 c is different from the tongue portion 470 and is not symmetrical. In other words, the bellows 470 and 470c may have any shape that allows force to be applied by a human hand during an automatic machine pin 610 or human work. Further, the number of bellows 470 and 470c is not limited to one, and a plurality of bellows may be provided. Furthermore, although the case where 1 set of the latching | locking part 460 and the latching rib 330 was demonstrated, it is not limited to this, You may provide multiple.

(Fifth embodiment)
Next, FIG. 15 is a schematic side view showing still another example of the lid member 200.

  As shown in FIG. 15, the lid member 200 d is provided with ribs 470 d as ribs perpendicular to the disk lid 410 instead of the tongue 470. Thereby, a force is applied to the tongue 470d by the pin 610 of the automatic machine, so that the lid member 200d can be shifted to an open state and a closed state. In the present embodiment, the case where the tongue portion 470d is a rib perpendicular to the disc lid 410 has been described. However, the present invention is not limited to this, or a member having a shape that intersects the disc lid 410, or It may be a member having any other shape.

  Subsequently, FIG. 16 is a schematic external view showing another example of the culture vessel set 100.

  In the culture vessel set 100a shown in FIG. 16, a lid 400 and a filter 550 are provided on a cylindrical portion 520 of the culture vessel 500. That is, the culture vessel set 100a shown in FIG. 16 is provided with the lid portion 400 integrally with the cylindrical portion 520.

  The lid 400 may be provided with the filter 550 and the hole 440, or may not be provided with the filter 550 and the hole 440.

  As described above, in the culture vessel set 100 according to the present embodiment, the lid member 200 includes the main body portion 300 and the lid portion 400. By opening only the lid 400 while the main body 300 is fitted to the culture vessel 500, the opening 320 of the main body 300 is opened, and the internal space and the external space of the culture vessel 500 can be easily communicated. Can do. Further, the tongue 470 is provided on the extension in the direction from the first hinge part 420 and the second hinge part 430 to the locking rib 330, i.e., protruding from the contact surface contacting the main body part 300. By applying a force to the bellows part 470, a large force can be applied to the locking rib 330 with the first hinge part 420 and the second hinge part 430 as rotation axes, and the locking rib 330 can be easily removed.

  Further, since the tongue portion 470 has a tongue shape, the locking rib 330 of the lid member 200 can be easily released by the pin 610 of the automatic machine in a state where cells or the like are cultured in the culture vessel 500. As a result, the culture medium in the inner space of the culture vessel 500 can be automatically replaced by opening the opening 320. That is, a large amount of the culture vessel 500 can be processed in a short time.

  Moreover, since the hole 440 is provided in the lid 400 and the filter 550 is provided in the hole 440, even when the lid 400 is locked to the main body 300, the inner space and the outer space of the culture vessel 500 Only clean air can be passed through. When exchanging the culture solution in the culture vessel 500, the lid 400 and the engaging portion 460 of the main body 300 are released to expose the opening 320, so that the culture vessel can be removed without removing the main body 300. The culture medium exchange in 500 can be performed.

  Further, the first hinge part 420 and the second hinge part 430 are positioned vertically above the center of the opening 320 (on the side opposite to the placement surface 530 of the culture vessel 500 with respect to the center of the opening 320). Therefore, when the lid member 200 is opened, the lid 400 is not disposed vertically below the opening 320. Therefore, when the culture medium in the culture vessel 500 is exchanged from the opening 320, even if there are various bacteria in the culture medium exchange nozzle 620 and the various bacteria fall downward, the contact surface side of the lid 400 is not touched. , Not a saucer. That is, the contact surface on the side of the opening 320 communicating with the inside of the culture vessel 500 does not serve as a tray, so that contamination of germs and the like into the culture vessel 500 can be reduced to the limit.

  Moreover, since the main body 300 and the lid 400 are provided as one body, it is possible to prevent the lid 400 from being lost. Further, the cost can be reduced by forming the main body unit 300 integrally.

  Moreover, in the culture vessel set 100a according to the present embodiment, the lid 400 is integrally formed with the culture vessel 500. By opening only the lid 400, the cylindrical portion 520 of the culture vessel 500 is opened, and the internal space and the external space of the culture vessel 500 can be easily communicated. Further, since the tongue portion 470 is provided so as to protrude from the contact surface that contacts the cylindrical portion 520, the engagement of the locking rib 330 can be easily released by applying a force to the tongue portion 470.

  In addition, it is preferable that the protrusion amount of the bellows parts 470, 470b, 470c, and 470d is 1 millimeter or more and 20 millimeters or less, and more preferably 3 millimeters or more and 15 millimeters or less.

  The protrusion amount of 1 mm or more is a minimum value in consideration of the size of the pin 610 of the automatic machine, and the protrusion amount of 20 mm or less is generally a buffer to an adjacently disposed container. Is a value that takes into account. For example, the culture container 500 has a width of 85 m, a height of 40 mm, a depth of 130 mm, and an outer diameter of the cylinder portion of about 30 mm. Buffering can be prevented.

  Further, when the protruding amount is less than the lower limit (less than 3 millimeters), the protruding amount is too small, and the pin 610 of the automatic machine is not stably in contact with the tongue portion, so that it is difficult to open and close. Further, when the protruding amount is larger than the upper limit value (15 millimeters), there is no problem because there is no buffering and margin for adjacent containers. If it is the said protrusion amount, since there is a margin, the buffer of an adjacent container can be prevented reliably and can be opened and closed by the pin 610 of an automatic machine.

  Further, when the protruding amount is less than the lower limit (less than 3 millimeters), the protruding amount is too small, and the pin 610 of the automatic machine is not stably in contact with the tongue portion, so that it is difficult to open and close. Further, when the protruding amount is larger than the upper limit value (15 millimeters), there is no problem because there is no buffering and margin for adjacent containers. If it is the said protrusion amount, since there is a margin, the buffer of an adjacent container can be prevented reliably and can be opened and closed by the pin 610 of an automatic machine.

  Further, the width of the bellows 470, 470b, 470c, 470d is preferably a width that can be opened and closed by the pin 610 of the automatic machine as described above, and is further 5 mm or more in width and 1.5 times or less of the width of the lid. It is preferable that When the width is less than 5 millimeters, the width is too small, and when opening and closing with the pin 610 of the automatic machine, the tongue portion is deformed and opening and closing becomes difficult. When it is larger than 1.5 times the width of the lid, it is not economical because the size of the production apparatus or automatic machine becomes relatively large. If it is the said width | variety, it will become possible to open and close by the pin 610 of an automatic machine.

In addition, the culture container 500 preferably has an internal volume in the range of 15 ml to 5000 ml. On the other hand, when the internal volume is 15 ml or less, the number of cells to be cultured is small, which is not efficient.
On the other hand, when the internal volume is 5000 ml or more, it is not preferable because the culture vessel 500 is too large and difficult to handle.

  In the present embodiment, the culture vessel 500 corresponds to the vessel, the opening 320 corresponds to the opening, the center of the opening 320 corresponds to the center of the opening, and the placement surface 530 serves as the placement surface. The main body 300 corresponds to the main body, the first hinge part 420 and the second hinge part 430 correspond to the connecting part, the lid part 400 corresponds to the lid part, and the locking rib 330 serves as the locking part. 1, the bellows portions 470, 470c, and 470d correspond to the protruding portions, the filter 550 corresponds to the filter member, the posture of the culture vessel 500 in FIG. Reference numeral 520 corresponds to the tube opening.

  The preferred embodiments of the present invention are as described above, but the present invention is not limited to them. It will be understood that various other embodiments may be made without departing from the spirit and scope of the invention. Furthermore, in this embodiment, although the effect | action and effect by the structure of this invention are described, these effect | actions and effects are examples and do not limit this invention.

DESCRIPTION OF SYMBOLS 100 Culture container set 100a Culture container set 200, 200a, 200b, 200c, 200d Lid member 300 Main body part 310 Housing 320 Opening part 330 Locking rib 340 Female screw 400 Lid part 410 Disc lid 420 First hinge part 430 Second hinge Portion 440 Hole 450 Sealing rib 460 Locking portion 470, 470c, 470d Velcro portion 510 Tubular outlet 520 Tubular portion 550 Filter 610 Automatic machine pin 620 Culture fluid exchange nozzle

Claims (18)

A lid member,
A body portion that fits into the container and has an opening;
A lid portion that is engaged with the main body portion by a connecting portion and that closes or opens the opening portion,
The lid is
A locking portion for locking the lid portion to the main body portion;
A container lid member, comprising: a protruding portion that protrudes from a contact surface that contacts the main body portion.   The container lid member according to claim 1, wherein the lid portion has a hole portion, and a filter member is disposed in the hole portion.   3. The container lid member according to claim 1, wherein the projecting portion is provided on at least an extension in a direction from the coupling portion to the locking portion.   The said protrusion part is plate shape, The cover member of the container of any one of Claim 1 to 3 characterized by the above-mentioned.   4. The container lid member according to claim 1, wherein the protruding portion has a tongue shape. 5.   The container lid member according to claim 1, wherein the main body portion and the lid portion are integrally provided. The container comprises a culture container or a centrifuge tube,
The opening part of the said main-body part has a dripping prevention part, The cover member of the container of any one of Claim 1 to 6 characterized by the above-mentioned.   8. The lid member for a container according to claim 7, wherein the dripping prevention part comprises a standing wall extending from the periphery of the opening of the main body part toward the lid part.   9. The dripping prevention part is provided in a range of about 180 degrees around the circumference of the opening part at a position away from the connection part around the opening part of the main body part. The lid member of the container described.   The container lid member according to claim 8, wherein when the lid portion closes the opening portion, the lid portion has a concave portion that sandwiches the standing wall. A container set,
The lid member according to any one of claims 1 to 10,
A container set including the container. When the lid member is attached to the container,
The container has a mounting surface for mounting on a table;
The container set according to claim 11, wherein the connecting portion of the lid member is disposed on the opposite side of the placement surface with respect to the center of the opening.   The container set according to claim 12, wherein the dripping prevention part is provided in a range of approximately 180 degrees around the circumference of the opening including at least the placement surface side.   The container set according to any one of claims 10 to 13, wherein the container is a culture container or a centrifuge tube.   The container set according to any one of claims 10 to 14, wherein the container is a flask or a roller bottle. Including a lid part integrally formed with the cylindrical mouth part of the container and having a connecting part;
The lid is
A locking portion for locking the lid portion to the tube opening of the container;
And a projecting portion provided so as to project from a contact surface that contacts the tube opening. The container comprises a culture container or a centrifuge tube,
The container according to claim 16, wherein the tube opening portion has a dripping prevention portion. The container has a mounting surface for mounting on a table;
The container according to claim 16 or 17, wherein the connecting portion of the lid member is disposed on the opposite side of the placement surface with respect to the center of the tube opening.