JP2019154264A - Container lid and culture container - Google Patents

Abstract

To provide a container lid and a culture container which can easily perform culture operation while suppressing contamination of a foreign matter into a container body.SOLUTION: A container lid 10 of a culture container is used in performing culture operation of culture solution C which is housed into a container body by a long-sized operation tool 100. The container lid 10 comprises: a cylindrical installing part 34 which is detachable from a port part 20 of the container body; and an opening/closing part 36 having an inner hole 39 which is provided for the installing part 34 and used for allowing an operation tool 100 to insert. The diameter of the inner hole 39 is reduced by rotating the opening/closing part 36 in one side of a circumferential direction with respect to the installing part 34 to cause deformation, and enlarged by rotating the opening/closing part 36 in the other side of a circumferential direction with respect to the installing part 34 to return twist.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a container lid and a culture container used when performing a culture operation of a culture solution stored in a container body using a long operation instrument.

  When culturing bacteria, cells, tissues, etc., culture containers are widely used. Patent Document 1 discloses a culture container provided with a container main body (flask) that contains a culture solution and a lid that can be attached to and detached from the mouth of the container main body.

Japanese Patent No. 3143419

  However, when performing a culture operation (culture liquid stirring operation, culture liquid addition operation, culture liquid recovery operation, etc.) using a culture container such as Patent Document 1 described above, the lid is removed from the container body, and the container It is necessary to insert an operation instrument such as a pipette from the mouth of the main body.

  At this time, the inner hole of the mouth portion is opened to the outside in a state where the operating instrument is inserted. For this reason, the user must prevent foreign matter from entering the container body. That is, the user can shorten the time required for the culturing operation as much as possible, prevent an object (such as a user's hand) from being positioned above the opened mouth, or prevent the operation tool from contacting the inner surface of the container body. There is a need to. That is, the user must pay attention to a plurality of things simultaneously during one culture operation.

  Further, in the above-described culture container, the operating tool cannot be supported with respect to the container body during the culture operation. If it does so, since a user must hold | maintain an operating instrument so that the inner bottom face of a container main body may not be damaged by the front-end | tip of an operating instrument, there exists a possibility that culture | cultivation operation cannot be performed easily.

  The present invention has been made in view of such problems, and an object of the present invention is to provide a container lid and a culture container that can easily perform a culture operation while suppressing the introduction of foreign matter into the container body. And

  In order to achieve the above object, a container lid according to the present invention is a container lid used when performing a culture operation of a culture solution contained in a container body with a long operation instrument, and the mouth of the container body A cylindrical mounting part that is detachable from the mounting part, and a cylindrical opening / closing part that is provided in the mounting part and has an inner hole through which the operating instrument is inserted, wherein the inner hole connects the opening / closing part. The diameter is reduced by rotating to the one side in the circumferential direction with respect to the mounting portion to reduce the diameter, and the diameter is increased by rotating the opening / closing portion to the other side in the circumferential direction with respect to the mounting portion to return the twist. It is characterized by doing.

  According to such a configuration, the user attaches the mounting part to the mouth part and twists and deforms the opening / closing part in a state where the operating instrument is inserted into the container body through the inner hole of the opening / closing part. By reducing the diameter of the hole, the opening / closing part can be brought into contact with the operating instrument. Therefore, the operating tool can be supported by the opening / closing part. Thereby, since the position of the operating instrument with respect to the container main body can be stabilized, the culturing operation can be easily performed. Moreover, when performing culture | cultivation operation, since the inner hole of an opening | mouth part can be covered with an opening-and-closing part, mixing of the foreign material in a container main body can be suppressed.

  Said container lid WHEREIN: The said opening / closing part may be comprised so that the shape torsionally deformed can be maintained so that the opening degree of the said inner hole can be adjusted.

  According to such a structure, the opening degree of the inner hole of the opening / closing part can be easily changed according to the thickness of the operating instrument.

  The container lid may include an operation portion that is provided in the opening / closing portion and is engaged with the mounting portion in a state where the inner hole is closed.

  According to such a configuration, the opening / closing part can be easily held in a state where the inner hole of the opening / closing part is closed.

  In the above container lid, the cylindrical operation part is connected to one end of the opening / closing part, the mounting part is connected to the other end of the opening / closing part, and the operation part is connected to the inside of the opening / closing part. You may fit to the said mounting part in the state by which the hole was obstruct | occluded.

  According to such a configuration, the operation unit can be locked to the mounting unit with a simple configuration.

  In the container lid, the opening / closing part may be provided with a deformation promoting part for promoting the torsional deformation.

  According to such a configuration, the opening / closing part can be easily twisted and deformed.

  A culture container according to the present invention includes a container body that contains a culture solution and the container lid described above.

  According to such a structure, the culture container which has the same effect as the container lid mentioned above can be obtained.

  According to the present invention, since the operating instrument can be supported by the opening / closing part, the culture operation can be easily performed. Moreover, when performing culture | cultivation operation, since the inner hole of an opening | mouth part can be covered with an opening-and-closing part, mixing of the foreign material in a container main body can be suppressed.

It is a perspective view of a culture container provided with a container lid concerning one embodiment of the present invention. FIG. 2 is a partially omitted longitudinal sectional view showing a state where the container lid of FIG. 1 is attached to the mouth. 3A is a side view of the container lid in a state where the inner hole of the opening / closing portion is fully opened, FIG. 3B is a plan view of the container lid of FIG. 3A viewed from the direction of arrow X1, and FIG. 3C is the opening / closing portion of FIG. 3D is a side view of the container lid in a state in which the opening degree of the inner hole is reduced, FIG. 3D is a plan view of the container lid of FIG. 3C viewed from the direction of arrow X1, and FIG. FIG. 3F is a plan view of the container lid of FIG. 3E viewed from the direction of arrow X1. It is a partially omitted cross-sectional view showing a state in which an operating instrument is inserted through the inner hole of the opening / closing part. It is sectional explanatory drawing of culture | cultivation operation. It is a side view of the container lid concerning a modification.

  Hereinafter, preferred embodiments of a container lid and a culture container according to the present invention will be described with reference to the accompanying drawings.

  As shown in FIG. 1, the culture container 12 is a container for culturing a culture target (bacteria, cells, tissues, etc.), and includes a container body 14, a culture lid 16, and a container lid 10.

  The container main body 14 contains a culture solution C (see FIG. 5) containing a culture target and a culture medium. The container body 14 is configured as a flask, for example. The container body 14 is integrally formed of a transparent material such as glass or plastic.

  The container main body 14 includes a storage portion 18 extending in one direction (arrow X direction) and a mouth portion 20 provided at one end of the storage portion 18 (end in the arrow X1 direction). The accommodating portion 18 is formed in a hexagonal shape in plan view. The accommodating portion 18 is formed with a constant width over a predetermined length from the other end (the end in the arrow X2 direction) toward the one end. One end side of the accommodating portion 18 is formed narrower toward the mouth portion 20.

  The accommodating portion 18 includes a bottom wall portion 22a, an inclined portion 22b, side wall portions 22c and 22d, end wall portions 22e and 22f, and a top wall portion 22g. The bottom wall portion 22a and the top wall portion 22g are formed in a flat plate shape and are arranged in parallel to each other. The inclined portion 22b is inclined from the one end (the end in the arrow X1 direction) of the bottom wall portion 22a toward the top wall portion 22g toward the side where the mouth portion 20 is located (see FIG. 5). That is, the inclined part 22b suppresses the culture solution C in the storage part 18 from leaking out of the mouth part 20 to the outside.

  The side wall portions 22c and 22d connect the left and right edges of the bottom wall portion 22a and the top wall portion 22g to each other. The end wall portion 22e connects one end of each of the inclined portion 22b, the top wall portion 22g, and the side wall portions 22c and 22d. The end wall portion 22f connects the other ends of the bottom wall portion 22a, the top wall portion 22g, and the side wall portions 22c and 22d to each other. The width dimension of the end wall part 22e (the dimension along the direction in which the side wall part 22c and the side wall part 22d are aligned) is shorter than the width dimension of the end wall part 22f. The mouth portion 20 is formed in a cylindrical shape and protrudes from the end wall portion 22e in the arrow X1 direction. A male screw portion 24 is formed on the outer peripheral surface of the mouth portion 20.

  The culture lid 16 is configured to be detachable from the mouth portion 20 and includes a cylindrical portion 26 and a disc portion 28 provided at an end portion of the cylindrical portion 26. On the inner surface of the cylindrical portion 26, a female screw portion 30 that is screwed into the male screw portion 24 of the mouth portion 20 is formed. The disk portion 28 is provided with a filter portion 32 that permits the flow of gas while preventing the flow of liquid.

  The container lid 10 is used when a culture operation of the culture solution C stored in the container main body 14 is performed by a long operation instrument 100 (see FIGS. 4 and 5) such as a pipette. Here, the culture operation refers to a stirring operation of the culture solution C, an additional operation of the culture solution C, a recovery operation of the culture solution C, and the like.

  The container lid 10 is a so-called disposable product, and is discarded after a predetermined number of times or a predetermined period of use. Specifically, the container lid 10 may be replaced with a new product every day (used a plurality of times) or may be replaced with a new product every time.

  As shown in FIGS. 1 and 2, the container lid 10 includes a cylindrical mounting part 34 that can be attached to and detached from the mouth part 20 of the container main body 14, an opening / closing part 36, and an operation part 37.

  The mounting portion 34 is formed in a cylindrical shape. The mounting portion 34 is made of a resin material. However, the constituent material of the mounting portion 34 can be arbitrarily set. An annular protrusion 38 (see FIG. 2) that is hooked on the male threaded portion 24 of the mouth portion 20 is provided on the inner surface of the mounting portion 34. However, the protrusion 38 is not limited to the example formed in an annular shape. That is, a plurality of protrusions 38 may be provided along the circumferential direction of the mounting portion 34. Further, an internal thread portion that is screwed into the external thread portion 24 may be formed on the inner surface of the mounting portion 34.

  The opening / closing part 36 is formed in a cylindrical shape and has an inner hole 39 provided in the mounting part 34 for allowing the operating instrument 100 to be inserted therethrough. One end portion (end portion in the arrow X1 direction) of the opening / closing portion 36 is fixed to the operation portion 37, and the other end portion (end portion in the arrow X2 direction) of the opening / closing portion 36 is fixed to the mounting portion 34. The opening / closing part 36 is made of, for example, a flexible rubber material such as silicone rubber.

  As shown in FIGS. 3A to 3F, the inner hole 39 of the opening / closing portion 36 is twisted by rotating the opening / closing portion 36 toward one side in the circumferential direction with respect to the mounting portion 34 (in the direction of arrow R1 in FIGS. 3B and 3D). The diameter is reduced by deforming. Further, the inner hole 39 of the opening / closing part 36 is expanded in diameter by rotating the opening / closing part 36 to the other side in the circumferential direction with respect to the mounting part 34 (in the direction of the arrow R2 in FIGS. 3D and 3F).

  The opening / closing part 36 is configured to maintain a twisted and deformed shape so that the opening degree of the inner hole 39 can be adjusted. When the opening / closing part 36 is made of silicone rubber, it is preferable that the opening / closing part 36 has a thickness of 0.2 mm or more and 1.0 mm or less because the opening / closing part 36 is easily maintained in a twisted state. However, the constituent material, thickness, and the like of the opening / closing part 36 can be arbitrarily set.

  As shown in FIGS. 1 and 2, the operation unit 37 is made of a hard resin material such as plastic. The operation unit 37 includes a cylindrical operation unit main body 40 and a fixing unit 42 provided on the operation unit main body 40. The outer diameter of the operation unit main body 40 is large enough for the user to operate with fingers.

  The inner diameter of the end portion in the arrow X2 direction of the operation portion main body 40 is substantially the same as the outer diameter of the end portion of the mounting portion 34 in the arrow X1 direction. That is, as shown in FIG. 3E, the end portion of the mounting portion 34 in the arrow X1 direction is fitted to the opening portion in the arrow X2 direction of the operation portion main body 40 in a state where the inner hole 39 of the opening / closing portion 36 is closed ( Insert). In other words, the operation portion main body 40 is locked to the mounting portion 34 in a state where the inner hole 39 of the opening / closing portion 36 is closed. In FIG. 2, the fixing portion 42 protrudes radially inward from the inner surface of the operation portion main body 40 and extends in the circumferential direction. One end of the opening / closing part 36 is fixed to the fixing part 42.

  Next, the effect | action of the culture container 12 which concerns on this embodiment is demonstrated. In the initial state, it is assumed that the culture medium C is stored in the container body 14 and the culture lid 16 is attached to the mouth portion 20. Further, it is assumed that the inner hole 39 of the opening / closing part 36 is fully opened.

  When performing a culture operation, the user removes the culture lid 16 from the mouth portion 20 and attaches the mounting portion 34 of the container lid 10 to the mouth portion 20. Then, the protrusion 38 of the mounting portion 34 gets over the male screw portion 24 over the male screw portion 24 of the mouth portion 20 in the arrow X2 direction. As a result, as shown in FIG. 2, the surface of the protrusion 38 that faces in the direction of arrow X <b> 1 abuts on the surface of the male screw portion 24 that faces in the direction of arrow X <b> 2. The

  Subsequently, the user inserts the operating instrument 100 into the container body 14 through the inner hole 39 of the opening / closing part 36. After that, as shown in FIG. 4, the user rotates the operation unit 37 to one side in the circumferential direction with the distal end portion of the operating instrument 100 positioned at a predetermined location in the storage unit 18, and opens and closes the opening / closing unit 36. Torsionally deform.

  Then, since the inner hole 39 of the opening / closing part 36 is reduced in diameter, the inner surface of the opening / closing part 36 is in contact with the outer surface of the operating instrument 100. Thereby, the operating instrument 100 is supported by the opening / closing part 36. In addition, the opening / closing part 36 covers the inner hole 21 of the mouth part 20 in a state where the operating instrument 100 is inserted into the inner hole 39.

  Thereafter, as shown in FIG. 5, the user performs a culture operation using the operation instrument 100. At this time, the movement of the operation instrument 100 in the direction orthogonal to the insertion direction of the operation instrument 100 with respect to the container body 14 is restricted by the opening / closing part 36. Therefore, the user can stabilize the operation instrument 100 with respect to the container body 14.

  When the culturing operation is completed, the user rotates the operation portion 37 to the other side in the circumferential direction to return the twist of the opening / closing portion 36 to expand the inner hole 39 of the opening / closing portion 36, thereby allowing the user to place the operation instrument 100 in the container. Remove from the body 14.

  Thereafter, for example, when continuously performing the culture operation, the user temporarily turns the inner hole 39 of the opening / closing portion 36 by rotating the operation portion 37 to one side in the circumferential direction to twist and deform the opening / closing portion 36. Block. Thereby, it can suppress that a foreign material mixes in the container main body 14 before performing next culture | cultivation operation. On the other hand, when the culture operation is not performed subsequently, the user removes the container lid 10 from the mouth portion 20 and attaches the culture lid 16 to the mouth portion 20.

  Next, effects of the container lid 10 and the culture container 12 according to the present embodiment will be described.

  The container lid 10 of the culture container 12 includes a mounting part 34 and a cylindrical opening / closing part 36. The opening / closing part 36 has an inner hole 39 provided in the mounting part 34 for allowing the operating instrument 100 to be inserted therethrough. The inner hole 39 is reduced in diameter by rotating the opening / closing portion 36 toward one side in the circumferential direction with respect to the mounting portion 34 and twisting and deforming, and rotates the opening / closing portion 36 toward the other side in the circumferential direction with respect to the mounting portion 34. To increase the diameter.

  Accordingly, the opening / closing portion 36 is twisted and deformed in a state where the user attaches the opening / closing portion 36 to the mouth portion 20 and inserts the operating instrument 100 into the container body 14 through the inner hole 39 of the opening / closing portion 36. By reducing the diameter of the inner hole 21 of the 36, the opening / closing part 36 can be brought into contact with the operating instrument 100. Therefore, the operating instrument 100 can be supported by the opening / closing part 36. Thereby, since the position of the operating instrument 100 with respect to the container main body 14 can be stabilized, culture | cultivation operation can be performed easily. Moreover, when performing culture | cultivation operation, since the inner hole 21 of the opening | mouth part 20 can be covered with the opening-and-closing part 36, mixing of the foreign material in the container main body 14 can be suppressed.

  The opening / closing part 36 is configured to maintain a twisted and deformed shape so that the opening degree of the inner hole 39 can be adjusted. Thereby, the opening degree of the inner hole 39 of the opening / closing part 36 can be easily changed according to the thickness of the operating instrument 100.

  The container lid 10 is provided in the opening / closing part 36 and has an operation part 37 that is engaged with the mounting part 34 in a state where the inner hole 39 is closed. Thereby, the opening / closing part 36 can be easily held in a state where the inner hole 39 of the opening / closing part 36 is closed.

  A cylindrical operation part 37 is connected to one end of the opening / closing part 36, and a mounting part 34 is connected to the other end of the opening / closing part 36. The operating portion 37 is fitted to the mounting portion 34 in a state where the inner hole 39 of the opening / closing portion 36 is closed. Therefore, the operation part 37 can be locked to the mounting part 34 with a simple configuration.

  The container lid 10 is not limited to the configuration described above. For example, as shown in FIG. 6, the opening / closing part 36 may be provided with a fold 44 as a deformation promoting part for promoting torsional deformation. In this case, the opening / closing part 36 can be easily twisted and deformed.

  Of course, the container lid and the culture container according to the present invention are not limited to the above-described embodiments, and various configurations can be adopted without departing from the gist of the present invention.

DESCRIPTION OF SYMBOLS 10 ... Container lid 12 ... Culture container 14 ... Container main body 20 ... Mouth part 21, 39 ... Inner hole 24 ... Male screw part 34 ... Mounting part 36 ... Opening / closing part 37 ... Operation part 38 ... Protrusion 44 ... Fold (deformation promotion part) 100 ... Operating equipment

Claims (6)

A container lid used when performing a culture operation of a culture solution stored in a container body by a long operation instrument,
A cylindrical mounting portion detachably attached to the mouth of the container body;
A cylindrical opening and closing portion provided in the mounting portion and having an inner hole for allowing the operation instrument to pass therethrough,
The inner hole is reduced in diameter by rotating the opening / closing portion to one side in the circumferential direction with respect to the mounting portion to cause torsional deformation, and the opening / closing portion is rotated to the other side in the circumferential direction with respect to the mounting portion. Let the twist expand and expand the diameter,
A container lid characterized by that. The container lid according to claim 1,
The opening / closing part is configured to maintain the twisted and deformed shape so that the opening of the inner hole can be adjusted.
A container lid characterized by that. The container lid according to claim 2,
Provided in the opening / closing part, and provided with an operation part for locking to the mounting part in a state where the inner hole is closed,
A container lid characterized by that. The container lid according to claim 3,
The cylindrical operation part is connected to one end of the opening / closing part,
The mounting part is connected to the other end of the opening / closing part,
The operation portion is fitted to the mounting portion in a state where an inner hole of the opening / closing portion is closed.
A container lid characterized by that. In the container lid according to any one of claims 1 to 4,
The opening / closing part is provided with a deformation promoting part for promoting the torsional deformation,
A container lid characterized by that. A container body containing a culture solution;
A container lid according to any one of claims 1 to 5,
A culture vessel characterized by that.

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