JP2019154262A - Container lid and culture container - Google Patents

Abstract

To provide a container lid and a culture container which can easily perform culture operation while suppressing contamination of a foreign matter into a container body.SOLUTION: A container lid 10A of a culture container 12 comprises: a cylindrical installing part 34 which is detachable to a port part 20 of a container body 14; and a lid body 36 provided for the installing part 34. The lid body 36 is constituted of an elastic material, and has an opening/closing part 42 in which a slit 40 for allowing an operation tool 100 to insert is formed, the slit 40 is opened by imparting pressing force in an inward direction along its extending direction with respect to the opening/closing part 42 and by elastically deforming the opening/closing part 42, and closed by restoring force of the opening/closing part 42 by removing the pressing force.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a container lid and a culture container used when performing a culture operation of a culture solution stored in a container body using a long operation instrument.

  When culturing bacteria, cells, tissues, etc., culture containers are widely used. Patent Document 1 discloses a culture container provided with a container main body (flask) that contains a culture solution and a lid that can be attached to and detached from the mouth of the container main body.

Japanese Patent No. 3143419

  However, when performing a culture operation (culture liquid stirring operation, culture liquid addition operation, culture liquid recovery operation, etc.) using a culture container such as Patent Document 1 described above, the lid is removed from the container body, and the container It is necessary to insert an operation instrument such as a pipette from the mouth of the main body.

  At this time, the inner hole of the mouth portion is opened to the outside in a state where the operating instrument is inserted. For this reason, the user must prevent foreign matter from entering the container body. That is, the user can shorten the time required for the culturing operation as much as possible, prevent an object (such as a user's hand) from being positioned above the opened mouth, or prevent the operation tool from contacting the inner surface of the container body. There is a need to. That is, the user must pay attention to a plurality of things simultaneously during one culture operation.

  Further, in the above-described culture container, the operating tool cannot be supported with respect to the container body during the culture operation. If it does so, since a user must hold | maintain an operating instrument so that the inner bottom face of a container main body may not be damaged by the front-end | tip of an operating instrument, there exists a possibility that culture | cultivation operation cannot be performed easily.

  The present invention has been made in view of such problems, and an object of the present invention is to provide a container lid and a culture container that can easily perform a culture operation while suppressing the introduction of foreign matter into the container body. And

  In order to achieve the above object, a container lid according to the present invention is a container lid used when performing a culture operation of a culture solution contained in a container body with a long operation instrument, and the mouth of the container body A cylindrical mounting part detachably attached to the part, and a lid body provided on the mounting part so as to cover an inner hole of the mouth part with the mounting part being attached to the mouth part, The lid main body has an opening / closing portion made of an elastic material and formed with a slit through which the operation instrument is inserted, and the slit is formed with respect to the opening / closing portion along the extending direction of the slit. The opening / closing part is released by elastically deforming it by applying a pressing force toward the direction, and is closed by the restoring force of the opening / closing part by removing the pressing force.

  According to such a configuration, the user removes the pressing force after the operating instrument is inserted into the container body in a state where the opening / closing portion is pressed and the slit is opened while the mounting portion is attached to the mouth portion. Thus, the operating tool can be supported by the opening / closing part. Thereby, since the position of the operating instrument with respect to the container main body can be stabilized, the culturing operation can be easily performed. Moreover, when performing culture | cultivation operation, since the inner hole of a mouth part is covered with the lid | cover main body, mixing of the foreign material in a container main body can be suppressed.

  In the container lid, the opening / closing portion includes a pair of lip portions provided on the mounting portion, and the pair of lip portions are close to each other in the axial direction of the mounting portion from the mounting portion. The slit may be formed by an extended end surface of the pair of lip portions.

  According to such a configuration, the slit can be opened relatively large and the configuration of the opening / closing part can be simplified.

  Said container lid WHEREIN: Each said lip | rip part may be extended toward the opposite side to the other end part from the one end part of the said mounting part.

  According to such a structure, since a lip | rip part can be arrange | positioned on the outer side of a mounting part, pressing force can be efficiently given to an opening-and-closing part.

  In the container lid described above, the lid body may include a filter unit that permits the flow of gas while preventing the flow of liquid.

  According to such a structure, the inside of a container main body can be ventilated, suppressing that the culture solution in a container main body leaks outside by a filter part.

  Said container lid WHEREIN: The said filter part may be adjacent to the said opening-and-closing part in the direction orthogonal to the extension direction of the said slit.

  According to such a structure, it can suppress that a filter part becomes obstructive when pressing an opening-and-closing part and opening a slit.

  In the above container lid, a male screw part may be formed on the outer peripheral surface of the mouth part, and a projection hooked on the male screw part may be provided on the inner surface of the mounting part.

  According to such a structure, a container lid can be latched with respect to a container main body by hooking a protrusion on an external thread part.

  The culture container according to the present invention includes a container main body that contains a culture solution and the container lid described above.

  According to such a structure, the culture container which has the same effect as the container lid mentioned above can be obtained.

  According to the present invention, since the operating instrument can be supported by the opening / closing part, the culture operation can be easily performed. Moreover, when performing culture | cultivation operation, since the inner hole of a mouth part is covered with the lid | cover main body, mixing of the foreign material in a container main body can be suppressed.

It is a perspective view of a culture container provided with a container lid concerning one embodiment of the present invention. FIG. 2 is a partially omitted longitudinal sectional view showing a state where the container lid of FIG. 1 is attached to the mouth. It is explanatory drawing which shows the operation | movement which inserts an operating tool into a slit. It is sectional explanatory drawing of culture | cultivation operation. 5A is a longitudinal sectional view of a container lid according to a first modification, FIG. 5B is a longitudinal sectional view showing a state in which the slit of the container lid of FIG. 5A is opened, and FIG. 5C is a second modification. FIG. 5D is a perspective view showing a state in which a slit of the container lid in FIG. 5C is opened.

  Hereinafter, preferred embodiments of a container lid and a culture container according to the present invention will be described with reference to the accompanying drawings.

  As shown in FIG. 1, the culture container 12 is a container for culturing a culture target (bacteria, cells, tissues, etc.), and includes a container body 14, a culture lid 16, and a container lid 10A.

  The container body 14 contains a culture solution C (see FIG. 4) containing a culture target and a culture medium. The container body 14 is configured as a flask, for example. The container body 14 is integrally formed of a transparent material such as glass or plastic.

  The container main body 14 includes a storage portion 18 extending in one direction (arrow X direction) and a mouth portion 20 provided at one end of the storage portion 18 (end in the arrow X1 direction). The accommodating portion 18 is formed in a hexagonal shape in plan view. The accommodating portion 18 is formed with a constant width over a predetermined length from the other end (the end in the arrow X2 direction) toward the one end. One end side of the accommodating portion 18 is formed narrower toward the mouth portion 20.

  The accommodating portion 18 includes a bottom wall portion 22a, an inclined portion 22b, side wall portions 22c and 22d, end wall portions 22e and 22f, and a top wall portion 22g. The bottom wall portion 22a and the top wall portion 22g are formed in a flat plate shape and are arranged in parallel to each other. The inclined portion 22b is inclined toward the top wall portion 22g from one end (the end in the arrow X1 direction) of the bottom wall portion 22a toward the side where the mouth portion 20 is located (see FIG. 4). That is, the inclined part 22b suppresses the culture solution C in the storage part 18 from leaking out of the mouth part 20 to the outside.

  The side wall portions 22c and 22d connect the left and right edges of the bottom wall portion 22a and the top wall portion 22g to each other. The end wall portion 22e connects one end of each of the inclined portion 22b, the top wall portion 22g, and the side wall portions 22c and 22d. The end wall portion 22f connects the other ends of the bottom wall portion 22a, the top wall portion 22g, and the side wall portions 22c and 22d to each other. The width dimension of the end wall part 22e (the dimension along the direction in which the side wall part 22c and the side wall part 22d are aligned) is shorter than the width dimension of the end wall part 22f. The mouth portion 20 is formed in a cylindrical shape and protrudes from the end wall portion 22e in the arrow X1 direction. A male screw portion 24 is formed on the outer peripheral surface of the mouth portion 20.

  The culture lid 16 is configured to be detachable from the mouth portion 20 and includes a cylindrical portion 26 and a disc portion 28 provided at an end portion of the cylindrical portion 26. On the inner surface of the cylindrical portion 26, a female screw portion 30 that is screwed into the male screw portion 24 of the mouth portion 20 is formed. The disk portion 28 is provided with a filter portion 32 that permits the flow of gas while preventing the flow of liquid.

  10 A of container lids are used when culture | cultivation operation of the culture solution C accommodated in the container main body 14 is performed with elongate operating instruments 100 (refer FIG.3 and FIG.4), such as a pipette. Here, the culture operation refers to a stirring operation of the culture solution C, an additional operation of the culture solution C, a recovery operation of the culture solution C, and the like.

  The container lid 10A is a so-called disposable product, and is discarded after a predetermined number of times or a predetermined period of use. Specifically, the container lid 10A may be replaced with a new product every day (used a plurality of times) or may be replaced with a new product every time.

  As shown in FIGS. 1 and 2, the container lid 10A is integrally formed of an elastic material. That is, the container lid 10A is made of a flexible resin material such as silicone rubber. 10 A of container lids are provided with the cylindrical mounting part 34 which can be attached or detached to the opening | mouth part 20 of the container main body 14, and the cover main body 36 provided in the mounting part 34. As shown in FIG.

  The mounting portion 34 is formed in a cylindrical shape. An annular protrusion 38 (see FIG. 2) that is hooked on the male threaded portion 24 of the mouth portion 20 is provided on the inner surface of the mounting portion 34. However, the protrusion 38 is not limited to the example formed in an annular shape. That is, a plurality of protrusions 38 may be provided along the circumferential direction of the mounting portion 34. In addition, an internal thread portion that engages with the external thread portion 24 may be formed on the inner surface of the mounting portion 34 in place of the protrusion 38.

  The lid body 36 is provided in the mounting portion 34 so as to cover the inner hole 21 of the mouth portion 20 in a state where the mounting portion 34 is attached to the mouth portion 20 (see FIG. 2). The lid main body 36 has an opening / closing part 42 in which a slit 40 for inserting the operating instrument 100 is formed.

  The opening / closing part 42 includes a pair of lip parts 44 provided on the mounting part 34. The pair of lip portions 44 extend from the mounting portion 34 so as to be close to each other in the axial direction of the mounting portion 34. Specifically, each lip portion 44 is inclined inward in the radial direction of the mounting portion 34 in the direction of the arrow X1.

  The slit 40 is formed by an extended end surface 45 of the pair of lip portions 44. The slit 40 is opened by applying a pressing force inward to the opening / closing portion 42 along the extending direction of the slit 40 and elastically deforming the opening / closing portion 42, and the pressing force is removed. It is blocked by the restoring force of the opening / closing part 42. In a state where the slit 40 is closed, the extended end surfaces 45 of the pair of lip portions 44 are in contact with each other.

  Next, the effect | action of the culture container 12 which concerns on this embodiment is demonstrated. In the initial state, it is assumed that the culture medium C is stored in the container body 14 and the culture lid 16 is attached to the mouth portion 20.

  When performing the culture operation, the user removes the culture lid 16 from the mouth portion 20 and attaches the mounting portion 34 of the container lid 10 </ b> A to the mouth portion 20. Then, the protrusion 38 of the mounting portion 34 gets over the male screw portion 24 over the male screw portion 24 of the mouth portion 20 in the arrow X2 direction. As a result, as shown in FIG. 2, the surface of the protrusion 38 that faces in the arrow X1 direction comes into contact with the surface of the male screw portion 24 that faces in the arrow X2 direction. The

  Subsequently, as shown in FIG. 3, the user applies a pressing force toward the inside with fingers with respect to the opening / closing part 42 from both sides in the extending direction of the slit 40. That is, the user presses the opening / closing part 42 from the direction of the arrow in FIG. Then, the lip portions 44 are elastically deformed in the direction in which the extended end surfaces 45 are separated from each other, so that the slits 40 are opened.

  In this state, the operating instrument 100 is inserted into the container body 14 from the slit 40. Thereafter, the user removes the pressing force applied to the opening / closing unit 42 in a state where the distal end portion of the operating instrument 100 is positioned at a predetermined location in the housing unit 18.

  Then, as shown in FIG. 4, the lip portion 44 tries to return to the original shape by the restoring force, so that the extended end surface 45 of the lip portion 44 contacts the outer surface of the operating instrument 100. Thereby, the operating instrument 100 is supported by the lip part 44 of the opening / closing part 42. The opening / closing part 42 covers the inner hole 21 of the mouth part 20 in a state where the operating instrument 100 is inserted through the slit 40.

  Thereafter, the user performs a culture operation using the operation tool 100. At this time, the movement of the operation instrument 100 in the direction orthogonal to the insertion direction of the operation instrument 100 with respect to the container body 14 is restricted by the lip portion 44. Therefore, the user can stabilize the operation instrument 100 with respect to the container body 14.

  When the culturing operation is completed, the user holds the operating instrument 100 in a state in which the hole of the slit 40 is widened by applying a pressing force inward to the opening / closing part 42 from both sides in the extending direction of the slit 40. Remove from the body 14. Thereafter, the user removes the pressing force applied to the opening / closing part 42. As a result, the slit 40 is closed by the restoring force of the opening / closing part 42. Then, the container lid 10 </ b> A is removed from the mouth portion 20, and the culture lid 16 is attached to the mouth portion 20.

  Next, effects of the container lid 10A and the culture container 12 according to the present embodiment will be described.

  The container lid 10 </ b> A of the culture container 12 includes a mounting portion 34 and a lid body 36. The lid body 36 is provided in the mounting portion 34 so as to cover the inner hole 21 of the mouth portion 20 in a state where the mounting portion 34 is attached to the mouth portion 20. The lid body 36 includes an opening / closing part 42 that is made of an elastic material and in which a slit 40 for inserting the operating tool 100 is formed.

  The slit 40 is opened by applying a pressing force inward to the opening / closing portion 42 along the extending direction of the slit 40 and elastically deforming the opening / closing portion 42, and the pressing force is removed. It is blocked by the restoring force of the opening / closing part 42.

  Accordingly, the operating instrument 100 is supported by the opening / closing section 42 by removing the pressing force after the user presses the opening / closing section 42 and the slit 40 is opened to insert the operating instrument 100 into the container body 14. can do. Therefore, since the position of the operation instrument 100 with respect to the container body 14 can be stabilized, the culture operation can be easily performed. Further, when the culture operation is performed, since the inner hole 21 of the mouth portion 20 is covered with the lid main body 36, it is possible to suppress the entry of foreign matters into the container main body 14.

  The opening / closing part 42 has a pair of lip parts 44 extending from the mounting part 34. The pair of lip portions 44 are inclined in a direction approaching each other in the axial direction of the mounting portion 34, and the slit 40 is formed by the extended end surfaces 45 of the pair of lip portions 44. Thereby, the slit 40 can be opened relatively large and the configuration of the opening / closing part 42 can be simplified.

  Each lip portion 44 extends from one end portion of the mounting portion 34 toward the side opposite to the other end portion. In this case, since the lip portion 44 can be disposed outside the mounting portion 34, it is possible to efficiently apply a pressing force to the opening / closing portion 42.

  Next, the container lids 10B and 10C according to the first and second modifications will be described. In the description of the container lids 10B and 10C, the same reference numerals are assigned to the same components as those of the container lid 10A described above, and detailed description thereof is omitted. In addition, the container lids 10B and 10C have the same operational effects with respect to the same configuration as the container lid 10A described above.

  As shown in FIG. 5A, the container lid 10B according to the first modification includes a mounting portion 34 and a lid body 36a. The opening / closing part 42a of the lid body 36a has a pair of lip parts 44a. The pair of lip portions 44 a extend from the mounting portion 34 toward the axial direction of the mounting portion 34 in directions close to each other. Specifically, each lip portion 44 a is inclined inward in the radial direction of the mounting portion 34 from one end portion of the mounting portion 34 toward the other end portion. That is, each lip portion 44 a extends toward the inner hole of the mounting portion 34. The slit 40 is formed by an extended end surface 45a of the pair of lip portions 44a.

  In such a container lid 10B, as shown in FIG. 5B, the opening / closing part 42a is elastically deformed by applying a pressing force to the opening / closing part 42a toward the inside of the mounting part 34 from the extending direction of the slit 40. As a result, the slit 40 is opened.

  As shown in FIG. 5C, the container lid 10C according to the second modification includes a mounting portion 34 and a lid body 36b. The lid body 36 b includes an opening / closing part 42 and a filter part 48. The opening / closing part 42 has a pair of lip parts 44 extending from one end of the mounting part 34.

  The filter unit 48 permits the flow of gas while blocking the flow of liquid. The filter part 48 is adjacent to the opening / closing part 42 in a direction orthogonal to the extending direction of the slit 40. In the container lid 10 </ b> C, as shown in FIG. 5D, a pressing force is applied to the opening / closing part 42 inward (along the arrow in FIG. 5D) from both sides in the extending direction of the slit 40 to open / close the part 42. The slit 40 is opened by elastically deforming.

  According to such a container lid 10 </ b> C, the inside of the container body 14 can be ventilated while suppressing the culture solution C in the container body 14 from leaking to the outside by the filter unit 48. That is, oxygen can be supplied to the culture target in the container body 14.

  Therefore, the container lid 10C can also be used as the culture lid 16 described above. That is, the container lid 10C can be attached to the mouth portion 20 of the container body 14 even when the culture operation is not performed. Therefore, the replacement work of the container lid 10C and the culture lid 16 can be reduced, and the number of parts can be reduced.

  Moreover, since the filter part 48 is adjacent to the opening / closing part 42 in a direction orthogonal to the extending direction of the slit 40, the filter part 48 becomes an obstacle when the opening / closing part 42 is pressed to open the slit 40. Can be suppressed.

  The container lid 10C is not limited to the above configuration. The filter part 48 may be arrange | positioned so that the opening-and-closing part 42 may be pinched | interposed from both sides. Moreover, the filter part 48 may extend in an annular shape so as to surround the outer periphery of the opening / closing part 42.

  Of course, the container lid and the culture container according to the present invention are not limited to the above-described embodiments, and various configurations can be adopted without departing from the gist of the present invention.

10A to 10C ... Container lid 12 ... Culture vessel 14 ... Container body 20 ... Mouth part 24 ... Male screw part 36, 36a, 36b ... Lid body 38 ... Projection 40 ... Slit 42, 42a ... Opening / closing part 44, 44a ... Lip part 45, 45a ... Extension end face 100 ... Operating instrument

Claims (7)

A container lid used when performing a culture operation of a culture solution stored in a container body by a long operation instrument,
A cylindrical mounting portion detachably attached to the mouth of the container body;
A lid body provided on the mounting portion so as to cover the inner hole of the mouth portion in a state where the mounting portion is mounted on the mouth portion,
The lid body is made of an elastic material and has an opening / closing part in which a slit for inserting the operation instrument is formed,
The slit is opened by applying a pressing force inward to the opening / closing part along the extending direction of the slit and elastically deforming the opening / closing part, and the pressing force is removed. Is closed by the restoring force of the opening and closing unit,
A container lid characterized by that. The container lid according to claim 1,
The opening / closing part has a pair of lip parts provided in the mounting part,
The pair of lip portions extends from the mounting portion so as to be close to each other in the axial direction of the mounting portion,
The slit is formed by an extended end surface of the pair of lip portions.
A container lid characterized by that. The container lid according to claim 2,
Each of the lip portions extends from one end portion of the mounting portion toward the opposite side to the other end portion,
A container lid characterized by that. The container lid according to any one of claims 1 to 3,
The lid body has a filter part that permits the flow of gas while preventing the flow of liquid,
A container lid characterized by that. The container lid according to claim 4,
The filter part is adjacent to the opening / closing part in a direction orthogonal to the extending direction of the slit,
A container lid characterized by that. In the container lid according to any one of claims 1 to 5,
A male screw part is formed on the outer peripheral surface of the mouth part,
On the inner surface of the mounting portion, a protrusion that is hooked on the male screw portion is provided.
A container lid characterized by that. A container body containing a culture solution;
A container lid according to any one of claims 1 to 6,
A culture vessel characterized by that.

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