KR100616200B1 - a microorganism culture tank - Google Patents

Abstract

The present invention relates to a culture tank for microbial solid culture and vacuum drying, so that the solid culture of the microorganism and the drying of the culture completed is all done in one tank. The present invention for this purpose is an apparatus for culturing microorganisms, comprising a culture tank having a chamber for solid culture by inoculating the microorganisms in the medium, the circumferential surface of the culture tank is cultured with hot water introduced from the hot water supply device A jacket for heating the inside of the tank is provided, and one side of the culture tank is connected to a vacuum suction tube, and a vacuum pump for applying a vacuum to the culture tank so that the moisture contained in the solid cultured culture evaporates to dry at a low temperature. It provides a microbial solid culture and vacuum drying culture tank characterized in that it is installed.

Microorganism, solid culture, vacuum drying, culture tank, vacuum pump

Description

Microbial solid culture and vacuum drying culture tank {a microorganism culture tank}

1 is a view showing a microbial solid culture and vacuum drying culture tank according to the present invention.

         <Description of the symbols for the main parts of the drawings>

10: culture tank 14: stirrer

16: jacket 18: hot water tank

20: steam injection device 22: nozzle

24: cold water supply device 26: spawn supply device

28: vacuum suction pipe 30: vacuum pump

32: cooling system

The present invention relates to a tank for culturing microorganisms, and more particularly, to a culture tank for microorganism solid culture and vacuum drying, wherein the solid culture of the microorganism and the drying of the culture completed are all performed in one culture tank.

As is well known, a method for culturing microorganisms is typically a liquid culture method and a solid culture method.

The latter solid culture method refers to the cultivation of microorganisms on a substrate containing water, although the medium in the culture tank is free to move freely, and compared to the liquid culture method, the following effects can be achieved. In the following, the advantages of the solid culture method will be described in comparison with the liquid culture method.

First, the medium used in the liquid culture method has a large volume of the medium itself because water occupies most of the medium, but as mentioned in the solid culture method, the volume of the medium in the medium may be small, so that the volume of the medium may be reduced. There is an advantage in that it is possible to reduce the space for the production and to obtain a production yield compared to the required space.

In addition, in order to obtain a product by liquid culture of microorganisms, most of the process requires a filtration step, and a large amount of liquid by-products (waste) generated in this step must be treated separately. The condensed product can be obtained without the advantage of simplifying the process.

Finally, liquid culture requires excessive energy and equipment cost due to complex equipment and separation process, while solid culture has advantages in terms of energy and cost by relatively simple equipment and easy separation process.

On the other hand, the microorganisms generated through the solid culture method is transferred to a drying apparatus, which is a separate post-treatment process, and then removes the water remaining in the product. Thus, the apparatus and drying apparatus for solid culture are respectively If it is provided separately will cause the following problems.

First, when the cultivation and drying is performed in each independent manufacturing apparatus, there is a problem that high facility investment cost and facility cleaning is cumbersome.

Next, microorganisms may be exposed to contaminants such as various germs or dusts during the transfer from the solid culture apparatus to the drying apparatus, which may not be able to obtain high-quality microorganisms to be sought and cause a problem in yield of large quantities of microorganisms.

Therefore, the present invention for solving the problems as described above is a microbial solid culture and vacuum that can reduce the investment cost of the microbial culture by making the solid culture of the microorganism, and drying the culture of the completed culture in one culture tank In providing a drying device.

In another aspect, the present invention is to provide a microbial solid culture and vacuum drying apparatus that can produce microorganisms of higher quality by preventing the microorganisms from being exposed to contaminants such as various bacteria or dust.

In another aspect, the present invention is to provide a microbial solid culture and vacuum drying apparatus that can improve the overall production yield.

The present invention for achieving the objects as described above, in the apparatus for culturing microorganisms, comprising a culture tank having a medium, and a chamber for solid culture by inoculating the microorganisms in the medium, the circumferential surface of the culture tank The jacket is provided with a jacket for heating the inside of the culture tank with hot water introduced from the hot water tank, one side of the culture tank is connected to the vacuum suction tube, the moisture contained in the solid culture culture is evaporated and dried at a low temperature The present invention provides a culture tank for microbial solid culture and vacuum drying, which has a technical point of installing a vacuum pump to apply a vacuum to the tank.

In another aspect, the present invention is a steam spraying device for sterilizing the medium by spraying steam through a nozzle installed in the culture tank, a cold water supply device for supplying cold water to the jacket to quench the medium, and discharge the microorganism through the nozzle Microbial solid cultivation and vacuum, characterized in that the seed feeding device to inoculate the medium, and installed in the culture tank and rotated by the power of the drive motor and agitator to agitate the microorganisms introduced from the seed feeding device with the medium Present a drying culture tank.

That is, the present invention will be characterized in that both the solid culture and the vacuum drying of the microorganisms are made in one device, preferably one culture tank, without having separate facilities for culturing and drying the microorganisms.

On the other hand, the solid culture to be referred to in the present invention refers to the culture of the microorganisms on the substrate containing no moisture, but free to move to the medium in the culture tank.

In addition, vacuum drying is a method of evaporating and drying moisture by indirectly heating a raw material by hot water and steam by using the principle that the break point of water is reduced under reduced pressure. For example, water boils at 100 ° C under 1 atmosphere, but the boiling temperature varies with pressure. That is, the lower the pressure, the weaker the pressure to press the water to boil at a lower temperature, and the higher the pressure, the stronger the pressure to press the water, so that it does not boil unless it reaches a high temperature. In other words, the vacuum drying method uses the property that the boiling temperature is lowered when the pressure is low. That is, by using the above-mentioned principle, the culture culture in the culture tank is completed in a vacuum pump, preferably a water-sealed vacuum pump to run a vacuum in the range of 10 ~ 199 torr in the culture tank is contained in the culture The water boils and evaporates at approximately 50-60 ° C. instead of 100 ° C. due to low pressure, so that the culture can be effectively dried without deterioration due to high temperatures. In addition, the water evaporated at the temperature point is discharged to the outside through the vacuum pump through the cooling process of the refrigerant system. And the device for heating the water is heated through the accessories such as hot water tank and jacket, a detailed description thereof will be described later.

Hereinafter, a detailed description of a preferred embodiment of the present invention will be described with reference to the accompanying drawings.

1 is a view showing the configuration of a microbial solid culture and vacuum drying apparatus to be implemented in the present invention.

Referring to FIG. 1, the microbial solid culture and vacuum drying apparatus includes one culture tank, a hot water tank, a steam spraying device, a cold water supply device, a seed supply device, a stirrer, a vacuum pump, and a cooling system.

The culture tank 10 is a tank for solid culture of microorganisms and vacuum drying of the culture completed, the chamber 12 is provided with a chamber 12 for receiving a proper amount of medium. Here, the medium is used after the raw material is selected or washed, and subjected to a pretreatment process such as steaming, roasting and crushing. In addition, the bottom surface of the culture tank 10 is provided with a stirrer 14, the stirrer 14 is rotated in accordance with the rotation of the shaft 15 is coupled to the axis of the drive motor 13 and the culture tank ( 10) The medium added into the mixture is stirred.

The hot water tank 18 supplies hot water to the culture tank 10, preferably the jacket 16 installed on the circumferential surface of the culture tank 10 to approximate the medium contained in the tank 10. It is first warmed to a temperature of about 70 ~ 80 ℃. In addition, the hot water tank 18 performs a function of heating the microorganisms to an appropriate temperature in the drying process of the cultured microorganisms.

The steam spraying device 20 sprays high temperature steam (about 110 to 120 ° C.) with the medium through the nozzle 22 installed in the culture tank 10 to sterilize the medium. At this time, the nozzle 22 performs a function of discharging the microorganisms stored in the seed feeder 26 to be described later in addition to steam as a medium.

The cold water supply device 24 supplies cold water instead of hot water into the jacket 14 to rapidly freeze the medium in the culture tank 10 to -30 ° C and perform sterilization. At this time, in order to supply the cold water into the jacket 14, it is obvious that the operation of returning the hot water into the hot water tank 18 must be preceded. On the other hand, when the cold water supply device 24 is operating gas in a high energy state in the culture tank 10 is liquefied to generate a predetermined vacuum in the culture tank 10, in this case nitrogen supply device ( 27) by aseptically supplying an appropriate amount of nitrogen into the culture tank 10, it is possible to suppress the generation of vacuum.

The seed feeding device 26 discharges the microorganism which is the seed through the nozzle 22 for spraying steam into the culture tank 10 to inoculate the medium. At this time, the seed feeding device 26 is preferably such that the microorganisms are discharged when the temperature in the culture tank 10 is maintained at a temperature of about 30 ℃ suitable for the growth of microorganisms. In addition, the method of maintaining the temperature in the culture tank 10 to about 30 ℃ is preferably to be adjusted through the above-described hot water tank 18 and the cold water supply device (24). In addition, the temperature in the culture tank 10 is automatically detected by the temperature sensing and control device 29 is installed in the culture tank (10).

On the other hand, after the inoculation of the microorganisms in the medium by the operation of the seed feeder 26 to operate the above-described stirrer 14 to stir the medium and the microorganisms. That is, the stirrer 14 is used not only for stirring the medium but also for stirring between the medium and the microorganism.

After the inoculation of the microorganisms into the medium by the operation of the above apparatus, after about 7 to 15 days, the sample obtained through the separate sampling port is analyzed to determine the solid culture completion point of the microorganism. In addition, as described above, the temperature and oxygen concentration must be maintained at all times in the culture tank 10 even during the period in which the microorganisms are cultivated, and the temperature is controlled through the hot water tank 18 and the cold water supply device 24 mentioned above. To this end, oxygen is controlled through an oxygen supply device 25 provided separately in the culture tank.

On the other hand, when the solid culture of the microorganisms through the above process is completed again by operating the hot water tank 18 to perform a drying operation to evaporate the moisture contained in the culture completed culture. At this time, the culture is transported into a separate drying tank without performing a drying operation in the culture tank 10 through the vacuum pump 30 and the hot water tank 18 mentioned above are connected to the culture tank 10. The drying operation is performed. In other words, the solid culture of the microorganisms in one culture tank 10 and drying of the culture completed.

In addition, in performing the drying operation in the culture tank 10, it is included in the culture to dry the culture in a state of lowering the pressure by applying a vacuum in the culture tank 10 through the vacuum pump 30 This is to prevent the deterioration of the culture in the process of heating to a high temperature by allowing the water to boil and evaporate at a low temperature, for example, 50 ~ 60 ℃.

That is, after the completion of the solid culture of the microorganisms in the culture tank 10, when performing a drying operation to evaporate the water contained in the culture, a state of continuously stirring the culture completed through the stirrer 14 first When the vacuum pump 30 is operated in the culture tank 10 through the vacuum suction pipe 28 takes a vacuum in the range of 10 ~ 199 torr and maintains a pressure lower than atmospheric pressure. At the same time, when the culture tank 10 is heated by supplying hot water stored in the above-described hot water tank 18 to the jacket 16, the moisture contained in the culture is 100 ° C. due to the low pressure applied to the culture tank 10. Instead of boiling at, it is boiled at 50-60 ℃ and evaporated to heat and dry the culture. In addition, the evaporated water, that is, the steam is sucked into the vacuum pump 30 through the vacuum suction pipe 28, and then discharged to the outside. 32) it is condensed in liquid state and discharged to the outside.

On the other hand, when the drying is completed as described above, the culture is discharged to the outside through the discharge port 34 is installed on the bottom surface of the culture tank (10).

Hereinafter, the operating state of the microorganism solid culture and vacuum drying apparatus of the present invention having the above configuration will be described with reference to FIG.

First, after sorting or washing the raw material, the medium subjected to the pretreatment process such as steaming, roasting, and crushing is introduced into the culture tank 10, the stirrer 14 is operated to perform the medium agitation.

Thereafter, the hot water tank 18 is operated to supply hot water to the jacket 16 installed on the circumferential surface of the culture tank 10 to warm the medium to about 60 to 70 ° C., and then the steam spraying device 20 It is operated to spray the medium having a temperature of about 110 ~ 120 ℃ to the medium through a nozzle 22 installed in the culture tank 10 to perform the sterilization of the medium.

Thereafter, the hot water supplied into the jacket 16 is returned to the hot water tank 18, and then the cold water supply device 24 is operated to supply cold water into the jacket 16 to rapidly freeze the medium at −30 ° C. Sterilize the medium completely.

Thereafter, the spawn feeder 26 is operated by maintaining the temperature in the culture tank 10 through the hot water tank 18 and the cold water supply device 24 at a temperature of about 30 ° C. suitable for the growth of microorganisms. When the microorganism is supplied into the culture tank 10 through the nozzle 22, the microorganism is inoculated into the medium.

Then, after stirring the medium and the microorganism with the stirrer 14, and after about 7 to 15 days, the sample is collected by the sampling port, and then the completion point of the culture is determined.

When the culture of the microorganism is completed through the above processes, the hot water tank 18 and the vacuum pump 30 are operated to perform drying of the culture completed in the culture tank 10. That is, when the vacuum pump 30 is operated, a vacuum in the culture tank 10 is applied to the culture tank 10 through the vacuum suction pipe 28 to maintain a pressure lower than atmospheric pressure. In this state, when the hot water tank 18 is operated to supply hot water into the jacket 16 of the culture tank 10 to heat the culture tank 10, the culture water is evaporated by the moisture contained in the culture. It is heated and dried in the culture tank (10). At this time, the water is boiled and evaporated at 50 to 60 ° C. instead of 100 ° C. due to the low pressure in the culture tank 10, and thus the risk of deterioration in the process of heating the culture to a high temperature can be excluded. In addition, as the moisture is evaporated at a low temperature, it is possible to reduce energy waste for heating the hot water tank 18.

Thereafter, the water evaporated through the above process, that is, the steam is sucked into the vacuum pump 30 through the vacuum suction pipe 28 and then discharged to the outside, wherein the steam passes through the vacuum suction pipe 28. In the process, it is condensed in a liquid state and discharged to the outside according to the operation of the cooling system 32 including the condenser.

On the other hand, the cultured through the drying operation as described above is discharged to the outside through the discharge port 34 installed on the bottom surface of the culture tank (10).

 As described above, the present invention achieves many effects as follows.

First, the solid culture of the microorganisms, and drying the culture of the solid culture is completed in one culture tank has the effect of reducing the equipment investment cost according to the microbial culture.

In addition, the microbial culture and drying are carried out in a closed culture tank without a separate transfer process, so that the microorganisms are not exposed to contaminants such as various germs or dusts, and thus, microorganisms are produced with the effect of producing higher quality microorganisms. Accordingly has a synergistic effect which can improve the overall production yield.

Claims (3)

In the culture tank for solid culture and vacuum drying of microorganisms so that the solid culture of the microorganisms and drying of the culture completed is all in one culture tank, A culture tank (10) having a medium and a chamber (12) for solid culture by inoculating the medium with microorganisms; The circumferential surface of the culture tank 10 is provided with a jacket 16 for heating the inside of the culture tank with hot water introduced from the hot water tank 18; One side of the culture tank (10) is connected to the vacuum suction tube 28, the vacuum that is applied to the culture tank 10 so that the moisture contained in the solid culture culture evaporated and dried at a low temperature during the heating process Microbial solid culture and vacuum drying culture tank, characterized in that the pump 30 is installed to include. According to claim 1, wherein the culture tank (10); A steam spraying apparatus 20 for spraying steam through a nozzle 22 installed in the culture tank 10 to sterilize the medium; A cold water supply device 24 for supplying cold water to the jacket 16 to quench the medium; A seed feeder 26 for discharging the microorganisms through the nozzle 22 to inoculate the medium; Microbial solid culture, characterized in that installed in the culture tank (10) and includes a stirrer (14) for rotating with the power of the drive motor 13 and the microorganisms introduced from the spawn supply device 26 and the medium and agitate And a culture tank for vacuum drying. The microorganism solid culture and vacuum drying culture according to claim 1 or 2, wherein the vacuum suction pipe 28 includes a refrigerant system 32 for condensing vapor sucked into the vacuum pump in a liquid state. Tank.

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