KR100821376B1 - Microorganism-incubator of indirectness sterilization - Google Patents

Abstract

A microorganism incubator is provided to maintain the optimum culturing state of the microorganism by forming a temperature controlling jacket at the outer circumferential side of a reactor and forming a temperature controlling device supplying thermal oil through an inlet and an outlet formed at the temperature controlling jacket and keep the temperature control regularly during microorganism culturing, thereby obtaining an accurate fermentation result, saving energy and preventing safety accident. A microorganism incubator comprises: a reactor(10) which consists of a shaft(12) connected to a motor(11) to rotate and a plurality of agitator(13), each of which is formed in a longitudinal direction of the shaft with spaced apart from the other agitator at regular interval and rotates to allow the microorganism to be metabolized in complete medium; a temperature controlling jacket(20) which is formed at the outer circumferential side of the reactor and has an inlet(21) and an outlet(22) at the upper and lower portions thereof to circulate thermal oil flown inside, thereby sterilizing various germs in the complete medium stored inside the reactor; a temperature controlling device which is interconnected to the inlet and outlet through a pipe and selectively supplies cooling water and stem to the thermal oil to heat-exchange them with the thermal oil; and an air supplying device(50) which flows outside purified air inside the reactor through an air pipe(51) formed from the upper outer circumferential side of the reactor to the inside lower end portion of the reactor, wherein the temperature controlling device comprises a stem heat exchanger, a cooling water heat exchanger, a temperature sensor, a circulation pump, an expansion chamber, a steam trap, and a controlling portion.

Description

Microorganism-incubator of Indirectness Sterilization

1 is a cross-sectional view showing a microbial incubator according to an embodiment of the present invention,

Figure 2 is a schematic diagram showing a temperature control device according to an embodiment of the present invention.

<Explanation of symbols for the main parts of the drawings>

10: reactor 20: jacket for temperature control

30: temperature controller 31: steam heat exchanger

32: cooling water heat exchanger 33: temperature sensor

34: circulation pump 35: expansion chamber

40: control unit 50: air supply device

100: incubator

The present invention relates to an indirect sterilized microorganism incubator, and more particularly, a temperature control jacket is formed on an outer circumferential surface of a reaction tank containing a medium and a microorganism, and the heat medium oil is supplied and exchanged through an inlet / outlet formed in the temperature control jacket. By forming the temperature control device to maintain the components of the medium to maintain the optimum culture state of the microorganisms, thereby obtaining the results of accurate fermentation, it is possible to maintain a constant temperature control during the culture of the microorganisms of steam The draw-in is focused on a certain part, and it can save energy by reducing excessive discharge.In this way, the mechanical load on the incubator is reduced, so it is easy to maintain, and the distortion caused by the negative pressure of the incubator reactor that can occur during sterilization is significantly lowered. Indirect sterilization microorganism incubator with reduced safety accidents .

In general, the incubator should be sterilized inside the reactor to provide certain conditions (cultivation of only specific microorganisms) in order to confirm the metabolic activity of the pure microorganism in the culture of the microorganism. Accordingly, in order to sterilize the inside of the reaction vessel, sterilization with steam connected to the reaction vessel, sterilization for a predetermined time, and aseptic state is then performed.

As a sterilization method for sterilization of the reaction tank, conventionally, most steam is injected into the outer circumferential surface of the tank or steam is directly added to all the lines to be directly injected into the tank for complete sterilization.

And, in order to send the minimum temperature necessary for sterilization to the entire reactor, a large amount of steam must be maintained at a high pressure and temperature through a certain line for a short time.

However, in order to send the minimum temperature necessary for sterilization to the entire reactor, a large amount of steam is maintained at a high pressure and temperature through a constant line for a short time, which not only causes a large strain on the incubator itself, Problems arise in obtaining accurate outputs of operators who want to obtain substantial target products (microorganisms) by bringing many modifications to the components of the culture medium.

In addition, by directly administering the steam to all the inlet line of the reactor, each element is configured around the inlet line by heat transfer, etc., and the steam injected at a high temperature and high pressure by the effect of the condensate discharged in a large amount of air Wet the air filter required for the purification system is shortened the life, there is a problem that a lot of material and repair costs accordingly.

As such, the sterilization of the reactor is not only a complicated and cumbersome process but also a fatal error affecting the most important culture components in the culture. In addition, it is difficult to maintain the correct volume due to the increase of condensate due to the inside of the reactor of the steam, it is difficult to obtain the exact target product by the deformation of the component due to the increase of the volume, as well as due to the heat of the steam coming from the initial inlet stage The problem is that the culture solution inside the site is pressed into the reactor and the components are destroyed, causing problems in the correct fermentation process.

Accordingly, the present invention has been made to solve the above conventional problems,

A temperature control jacket is formed on the outer circumferential surface of the reaction vessel containing the medium and the microorganism, and a temperature control device for supplying and heat-exchanging the heat medium oil through the inlet / outlet formed in the temperature control jacket is formed, thereby maintaining the components of the medium to optimize the microorganism. It is possible to maintain the culture state of, thereby obtaining the result of accurate fermentation.

In addition, the present invention can maintain a constant temperature control when culturing microorganisms, the inflow of steam is focused only on a certain portion can reduce excessive emissions, energy saving, as described above, mechanical maintenance is reduced in the incubator, easy maintenance In addition, the distortion caused by the negative pressure of the incubator reactor that can occur during sterilization is significantly lowered, there is another purpose to reduce the safety accident.

The present invention has the following features to achieve the above object.

The present invention is a microorganism incubator,

A reaction tank comprising a rotating shaft connected to a motor formed on the upper side and rotating, and a stirrer rotating at a predetermined interval in the longitudinal direction of the rotating shaft so as to rotate the microorganisms in a complete medium;

A temperature control jacket formed on an outer circumferential surface of the reaction tank and having an inlet / outlet formed at upper and lower ends thereof so as to circulate heat medium oil introduced therein to sterilize various bacteria in the medium stored in the reactor;

A temperature control device connected to an inlet / outlet of the temperature control jacket to heat-exchange the cooling water and steam to control the temperature inside the reactor;

It characterized in that it comprises an air supply device for the outside of the purified air to enter the inside of the reaction tank through the air pipe formed through the upper outer peripheral surface of the reaction tank to the inner lower end of the reaction tank.

The present invention having such a feature will be more clearly described through the preferred embodiment accordingly.

Hereinafter, preferred embodiments of the present invention will be described in detail with the accompanying drawings.

1 is a cross-sectional view showing a microbial incubator according to an embodiment of the present invention, Figure 2 is a schematic diagram showing a temperature control apparatus according to an embodiment of the present invention.

As shown in FIG. 1, the indirect sterilization microorganism incubator 100 of the present invention includes a reaction tank 10, a temperature control jacket 20, a temperature control device 30, and an air supply device 50. do.

The reactor 10 has a motor 11 is formed on the upper side, is connected to the lower side of the motor 11 is formed with a rotary shaft 12 perpendicular to the inside of the reaction tank 10, the rotary shaft 12 is Rotation is driven by the motor 11 and the end portion penetrates the inside of the reaction vessel 10 and is coupled to the bottom surface of the reaction vessel 10.

Here, a plurality of stirrers 13 are formed at regular intervals in the longitudinal direction of the rotary shaft 12, and the stirrer 13 is simultaneously rotated by the rotation of the rotary shaft 12 to be introduced into the reactor 10. Purified air introduced through the air supply device 50 to the medium (prey of the microorganisms) is mixed evenly or mixed through rotation to allow the microorganisms to be cultured in the sterilized medium to be metabolized. At this time, the medium is formed in a sterile state by heating, the heat directly through the temperature control jacket 20 formed on the outer circumferential surface of the reaction tank 10 to indirectly transfer heat without contacting the medium or microorganisms.

The temperature control jacket 20 is formed to surround the reaction tank 10 in the longitudinal direction on the outer circumferential surface of the reaction tank 10, the water (heat medium oil) is filled in the inside of the temperature control jacket 20 is the reaction tank ( 10) in order to indirectly sterilize the germs in the medium stored in the inside of the medium by spraying hot steam to water (thermal medium oil) to heat the circulated water, and the water is converted into high temperature heat medium oil to heat the inside of the reactor To pass. (The above water and heat medium oil are the same and are used repeatedly below.)

Here, by indirectly transferring heat to sterilize the medium through the temperature control jacket 20, heat is directly contacted with the medium to prevent the components of the medium from being destroyed, whereby stable microorganisms can also be cultured stably. . At this time, in order to completely sterilize the medium introduced into the reactor 10, the inside of the reactor 10 should be maintained at a temperature of 121 ° C. for at least 30 minutes. The reason is that various germs (other than microorganisms to be cultured) which are parasitic in the medium are sterilized by heating at least 120 ° C for at least 30 minutes. To this end, the temperature of steam for heating the cooling water filled in the temperature control jacket 20 is formed at 120 to 150 ° C.

As such, the temperature control jacket 20 is connected to the temperature control device 30 so that water (heat medium oil) flows into the inside, and the lower end of the temperature control jacket 20 so that the temperature control device 30 is connected. The inlet 21 is formed, the outlet 22 is formed on the outer peripheral surface of the upper end of the temperature control jacket 20.

The air supply device 50 is formed to introduce the air purified from the outside into the reaction tank 10, the air pipe 51 is formed so that the air is transported, the air pipe 51 of the reaction tank 10 It penetrates through the upper outer circumferential surface and is formed up to an inner lower end of the reactor 10. At this time, the end of the air pipe 51 is formed in the inner lower end of the reaction tank 10, so that the introduced air flows from the bottom to the top.

Referring to Figure 2, the temperature control device 30 is connected to the inlet / outlet 21, 22 of the temperature control jacket 20, the steam for heat-exchanging the heat medium oil and steam to control the temperature inside the reaction tank 10 It consists of a heat exchanger 31, a cooling water heat exchanger 32, a temperature sensor 33, a circulation pump 34, an expansion chamber 35, and a control part 40.

Here, the steam heat exchanger 31 is a pipe (P3) is connected to one side of the steam heat exchanger 31 so that the external steam is introduced to transfer heat to the heat medium oil formed inside the temperature control jacket 20, In the pipe P3, a valve V2 is formed to control external steam. In addition, a pipe P8 is connected to the outlet 22 so that the heat medium oil discharged through the outlet 22 of the temperature control jacket 20 is transferred, and the pipe P8 is an outer peripheral surface of the pipe P4. Branched to the pipe (P4) is connected to one side of the steam heat exchanger 31 to transfer the heat medium oil discharged through the outlet 22 of the temperature control jacket 20 to the steam heat exchanger (31). . The pipe P7 is connected to another side of the steam heat exchanger 31, and the pipe P7 includes steam traps 36 formed at one end of the pipe P7. The condensate is discharged to the outside by the control of.

In addition, the coolant heat exchanger 32 is connected via a pipe P5 formed on one side of the steam heat exchanger 31, and a pipe P1 for transferring external coolant is connected to one side of the coolant heat exchanger 32. The pipe P1 is branched at a predetermined portion so as to be connected to one side of the coolant heat exchanger 32 and the inlet 21 of the temperature control jacket 20 so that external coolant does not pass through the coolant heat exchanger 32. It is controlled by the valve (V1) to be introduced into the temperature control jacket 20 immediately. Then, after the heat medium oil (water) introduced into the coolant heat exchanger 32 through the pipe P5 and the coolant are exchanged with heat, one end of the pipe P1 is connected to the outer peripheral surface of the pipe P1 to be supplied to the temperature control jacket 20. Pipe (P2) is connected to another side of the cooling water heat exchanger, the pipe (P6) for transporting so that a part is discharged to the outside after the cooling water introduced through the pipe (P1) heat exchange, the cooling water heat exchanger (32) Is connected to one side of, the pipe (P6) is formed with a valve (V3) to adjust the cooling water discharged to the outside.

In addition, the temperature sensor 33 is formed at a portion where the pipe P8 and the pipe P4 are connected to measure the temperature of the heat medium oil discharged through the outlet 22 of the temperature control jacket 20 to the reaction tank 10. Determine the internal temperature of the, and transmits the electrical signal to the control unit 40 the temperature data measured by the temperature sensor 33.

Then, the control unit 40 is to control the respective valves (V1 ~ V3) and the circulation pump 34 so that the temperature is kept constant by determining the inside of the reaction tank (10). Here, the circulation pump 34 is installed at one end of the pipe (P4) to circulate the cooling water and steam transferred from each of the pipes (P1 ~ P8).

The expansion chamber 35 is installed at an upper end of the pipe P4 (opposite side of the pipe P4 connected to the steam heat exchanger 31) to adjust the pressure in the entire pipes P1 to P8. A pressure gauge is installed at one end of the expansion chamber 35 so that the user can visually check it.

Hereinafter, the method of operating the indirect sterilized microorganism incubator 100 for the present invention described above will be described together with the accompanying drawings.

An operation method described below will be described with reference to FIG. 2.

Operation method of temperature control according to the sterilization of the medium introduced into the reaction tank 10 of the incubator 100,

First, after the valve V1 is opened to fill and circulate the water inside the temperature control jacket 20, external water (heat medium oil) fills the inside of the temperature control jacket 20 through the pipe P1. The valve V1 is closed by the signal of the control unit 40 and the circulation pump 34 is operated so that the water is piped (P4) → pipe (P5) → pipe (P2) → pipe (P1) → jacket for temperature control (20). → circulates in the direction of pipe (P8) → pipe (P4).

Then, by opening the valve (V2), the external steam flows into the steam heat exchanger 31 through the pipe (P3), through the pipe (P4) and the pipe (P5) in the steam heat exchanger (31). The water and the steam to be transported is heat exchanged to supply steam until the internal temperature of the reaction tank 10 reaches 121 ° C., and the condensed water generated in the steam heat exchanger 31 is moved through the pipe P7 to form a steam trap ( It is discharged to the outside through 36), when the internal temperature of the reaction tank 10 reaches 121 ℃ to transmit the temperature value of the water measured by the temperature sensor (4 ~ 20mA) to the control unit 40 to maintain the temperature for 30 minutes Control / Adjust

Finally, the internal temperature of the reaction vessel 10 is maintained at 121 ℃ for 30 minutes to close the valve (V2) at the time when the sterilization is finished.

As described above, after sterilizing the medium, the operating method for cooling the inside of the incubator 100 to incubate microorganisms,

In order to culture the microorganisms in the medium, the temperature at which the microorganisms are cultured (25 ° C. to 35 ° C.) must be maintained. To do so, when sterilized, it is necessary to cool the temperature inside the incubator 100.

First, the valve V3 is opened and discharged through the pipe P6 to return the coolant introduced from the outside, and the external coolant is introduced through the pipe P1 connected to the coolant heat exchanger 32, and the coolant heat exchanger is performed. The temperature in the reaction tank 10 is lowered by heat-exchanging the water and the cooling water circulated by the circulation pump 34 in the vessel 32, and the temperature in the reaction tank 10 is the culture temperature (25 ° C to 35 ° C). If lower, the valve (V2) is opened to supply the external steam to increase the temperature.

As such, the valve V3 and the valve V2 are alternately operated by the signal of the controller 40 to control / control the temperature to satisfy the optimum requirement for microorganisms to be cultured.

As described above, the indirect sterilized microbial incubator of the present invention is formed on the outer peripheral surface of the reaction tank containing the medium and microorganisms, the temperature control jacket is formed, and the heat medium oil supply and heat exchange through the inlet / outlet formed in the temperature control jacket By forming the temperature control device, the components of the medium are maintained to maintain the optimum culture state of the microorganisms, thereby resulting in the effect of obtaining the result of accurate fermentation.

In addition, the present invention can maintain a constant temperature control when culturing microorganisms, the inflow of steam is focused only on a certain portion can reduce excessive emissions, energy saving, as described above, mechanical maintenance is reduced in the incubator, easy maintenance In addition, the distortion caused by the negative pressure of the incubator reactor that can occur during sterilization is significantly lowered, thereby reducing the safety accident.

Claims (2)

In a microbial incubator, The rotating shaft 12 is connected to the motor 11 formed on the upper side and rotates, and a plurality of spaced apart at regular intervals in the longitudinal direction of the rotating shaft 12 so that the microorganisms in the medium (complete medium) can metabolize activity A reaction tank 10 composed of a rotating stirrer 13; Is formed on the outer circumferential surface of the reaction tank 10, the inlet / outlet 21, 22 are formed in the upper, lower so as to circulate the heat medium oil introduced therein to sterilize the bacteria in the medium stored in the reactor 10 A temperature control jacket 20; The inlet / outlet ports 21 and 22 of the temperature control jacket 20 are interconnected by using pipes, and selectively supply cooling water and steam to the heat medium oil filled therein to control the temperature inside the reactor 10. A temperature control device 30 for heat exchange with the heat medium oil; An air supply device (50) through which the outside purified air flows into the reaction tank (10) through an air pipe (51) formed through the upper outer circumferential surface of the reaction tank (10) to an inner lower end of the reaction tank (10); Indirect sterilized microbial incubator, characterized in that comprising a. According to claim 1, wherein the temperature control device 30, Pipe (P3) is supplied to the outside steam is controlled by the control of the valve (V2) is connected to one side, the heat medium oil discharged from the outlet 22 of the temperature control jacket 20 is discharged through the pipe (P8) The pipe P4 is connected to one side so that the pipe P4 is connected to the pipe P4 connected to the pipe P8, and a pipe P7 through which condensed water is discharged through steam introduced through the pipe P3 is connected. A steam heat exchanger 31; One side is connected through a pipe (P5) formed on one side of the steam heat exchanger (31), a pipe (P1) supplied by the external cooling water by the control of the valve (V1) is connected to one side, the pipe (P5) Pipe (P2) connected to one end of the pipe (P1) is connected to one side so that the cooling water introduced through the temperature control jacket 20 is connected to one side, and after the heat exchange the cooling water introduced through the pipe (P1), the outside Cooling water heat exchanger 32 is connected to one side of the pipe (P6) discharged by the control of the furnace valve (V3); A temperature sensor 33 formed at a portion at which the pipe P8 and the pipe P4 are connected to measure an internal temperature of the reaction tank 10 through cooling water; A circulation pump 34 installed in the pipe P4 for circulating cooling water and steam; An expansion chamber (35) installed at an upper end of the pipe (P4) to adjust pressure in the entire pipe; A control unit 40 which receives the signal of the measured data to the temperature sensor 33 to operate / control the valves V1 to V3, the steam trap 36, and the circulation pump 34; Indirect sterilized microbial incubator, characterized in that comprising a.

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