WO2013119072A1 - Microorganism cultivator for inducing activation of anaerobic digestion of organic waste - Google Patents

Microorganism cultivator for inducing activation of anaerobic digestion of organic waste Download PDF

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Publication number
WO2013119072A1
WO2013119072A1 PCT/KR2013/001023 KR2013001023W WO2013119072A1 WO 2013119072 A1 WO2013119072 A1 WO 2013119072A1 KR 2013001023 W KR2013001023 W KR 2013001023W WO 2013119072 A1 WO2013119072 A1 WO 2013119072A1
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WIPO (PCT)
Prior art keywords
culture
culture solution
gas
culture vessel
anaerobic digestion
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PCT/KR2013/001023
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French (fr)
Korean (ko)
Inventor
배성수
김태룡
Original Assignee
에스케이케미칼 주식회사
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Publication of WO2013119072A1 publication Critical patent/WO2013119072A1/en

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/107—Apparatus for enzymology or microbiology with means for collecting fermentation gases, e.g. methane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/02—Temperature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • the present invention relates to a microorganism incubator for inducing anaerobic digestion of organic wastes, and more particularly, to a microorganism incubator for inducing anaerobic digestion of organic wastes to activate methane fermentation microorganisms of a culture solution containing organic contaminants. .
  • anaerobic digestion processes such as single phase, double digestion or mesophilic digestion are commonly used for stabilization.
  • Digestion gas produced through the existing anaerobic digestion process is separated into carbon dioxide and methane, methane is reused as the energy source of the boiler, and carbon dioxide is compressed and stored.
  • the present invention has been made in order to solve the above problems, to supply the culture solution containing the organic contaminants (solution to activate the anaerobic microorganisms) by activating anaerobic microorganisms to the anaerobic digestion process, It is an object of the present invention to provide a microorganism incubator for inducing anaerobic digestion activity of organic waste, which can be uniformly stirred with a culture solution at the same time as supplying a carbon dioxide gas substrate for propagation.
  • the present invention provides a hollow culture vessel having a culture solution supply pipe into which the culture solution is introduced from the outside; Hollow first and second gas supply heads spaced vertically apart from each other in the center of the culture vessel and receiving a carbon dioxide gas substrate from a gas supply blower; A plurality of first injection nozzles connected to the outer circumferential surface of the first gas supply head and disposed downwardly at an angle toward the inner wall surface of the culture vessel; It is connected to the outer circumferential surface to the second gas supply head, a plurality of second injection nozzles are disposed upward at a predetermined angle toward the center portion of the culture vessel; and comprises a gas substrate of the first injection nozzle of the culture vessel Induction of anaerobic digestion of organic waste, characterized in that the injection downward to the inner wall surface and the gas substrate of the second injection nozzle is injected upward to the center of the culture vessel, the gas substrate is injected into the culture solution and the culture solution is stirred. It provides a microbial incubator.
  • the culture vessel has a heating tube for adjusting the temperature of the culture solution introduced into the culture vessel, the heating tube is located in a submerged form in the culture solution.
  • the heating tube is configured to be connected to a cold medium supply means for supplying a refrigerant or fruit for heat exchange with the culture solution
  • the cold medium supply means is connected to the heating tube from the outside of the culture vessel to store and store the refrigerant or fruit It is installed between the fruit tank, the coolant tank and the heating tube, and is installed in the coolant circulation pump for circulating and supplying the refrigerant or the fruit of the coolant tank to the heating tube according to the signal of the controller, and is installed in the coolant tank to bring the fruit to a constant temperature.
  • It consists of a culture heater for raising the temperature.
  • a gas preheating heater is configured between the gas supply head and the gas supply blower to preheat the carbon dioxide gas substrate supplied to the gas supply head.
  • the culture vessel has a sterilization heater for sterilizing the inside of the culture vessel at a high temperature after discharging the culture solution at the bottom, and a washing water spray nozzle for washing the interior of the culture vessel at the top.
  • the culture solution supply pipe is connected to the culture solution supply pump for supplying the culture solution supplied from the outside, the bottom of the culture vessel culture for discharging the culture solution in which the microorganisms to be cultured to the outside
  • the solution discharge line is connected.
  • Microorganism incubator for inducing anaerobic digestion activity of organic wastes by directly introducing a culture solution for culturing methane fermentation microorganisms (that is, anaerobic microorganisms) can be easily cultured microorganisms in the field.
  • the microbial incubator of the present invention can activate the anaerobic digestion of organic wastes by recycling the culture solution in which the anaerobic microorganisms have been grown and supplying them to the anaerobic digestion tank of the anaerobic digestion process for the treatment of organic wastes.
  • the low methane production yield during the anaerobic digestion process can reduce the methane production yield, or the methane fermentation period is long, the anaerobic digestion efficiency can be improved.
  • methane gas separated from the digestion gas during the anaerobic digestion process is used for energy reproduction such as thermal power and electricity, and carbon dioxide gas can be reused as a gas substrate of the microbial incubator of the present invention, thereby improving the energy utilization rate of the entire anaerobic digestion process. You can increase it.
  • FIG. 1 is a schematic configuration diagram showing a microbial incubator for inducing anaerobic digestion activity of an organic waste according to an embodiment of the present invention
  • Figure 2 is a schematic view showing a part of the microorganism incubator for inducing anaerobic digestion of organic waste according to an embodiment of the present invention
  • FIG. 3 is a plan view partially showing a microorganism incubator for inducing anaerobic digestion activity of an organic waste according to an embodiment of the present invention
  • Figure 4 is an enlarged cross-sectional view of the culture vessel lower portion of FIG.
  • FIG. 5 is a flowchart illustrating an anaerobic digestion process using a microbial incubator for inducing anaerobic digestion activity of organic wastes according to the present invention.
  • the microbial incubator 100 for inducing anaerobic digestion activity of the organic wastes is to culture in the culture vessel 20, the culture vessel 20 is made of the culture of anaerobic microorganisms While supplying a carbon dioxide gas substrate into a supply unit 1 and a culture vessel 20 for supplying a culture solution (a solution for culturing and proliferating / activating anaerobic microorganisms, for example, organic waste, etc.) and an organic medium and an inorganic medium. It comprises a stirring part (2) for stirring the culture solution, and a washing sterilization part (5) for preventing contamination.
  • the anaerobic digestion activity induction microorganism incubator 100 is installed in the culture vessel 20, the temperature control unit for controlling the temperature conditions of the culture solution to form the optimal temperature conditions for the culture of microorganisms in the culture solution (3) And a gas control unit 4 for controlling the temperature of the carbon dioxide gas substrate supplied into the culture vessel 20 to an appropriate temperature.
  • the culture vessel 20 is a hollow cylindrical water tank, which is supported by a base frame (not shown) and installed in a vertical direction, and is provided with a heat insulating material 25 made of polyurethane or the like on an outer surface thereof, and an upper portion thereof.
  • the medium is provided with a medium inlet 21 for injecting the strain and the medium.
  • the discharge inlet 21 is provided with a cover 22 to open and close the discharge inlet 21 when necessary.
  • the culture vessel 20 is a level gauge 23 made of a transparent tube in the axial direction of the culture vessel 20 can be visually confirmed the water level inside the culture vessel 20, through this culture vessel The level of 20 can be detected and adjusted automatically.
  • a temperature indicator (not shown) is installed on one side of the culture vessel 20 to check the temperature of the culture solution, and a culture solution discharge tube 24 for discharging the culture solution completed on the bottom of the culture vessel 20. ) Is installed connected.
  • the supply unit 1 is installed to be connected to the culture vessel 20, the culture solution supply pipe 30 for injecting the culture solution into the culture vessel 20, and the culture solution supplied from the culture solution supply pipe 30 It comprises a culture solution supply pump 31 for supplying to, and may include a valve and a pressure gauge to open and close the culture solution supply pipe (30).
  • the culture solution supply pump 31 is fixedly installed on the base frame and is connected to the culture solution supply pipe 30 to supply the culture solution to the culture vessel 20 via the culture solution supply pipe 30.
  • the culture solution supply pump 31 is connected to the aeration tank of the wastewater treatment plant located outside the microorganism incubator 100 and the like, and raises the aeration solution of the wastewater treatment plant and the like through the culture solution supply pipe 30. ) Will be supplied internally.
  • the stirring unit 2 is installed between the gas supply blower 40 and the gas supply blower 40 and the culture vessel 20 fixedly installed on one side of the base frame to provide a carbon dioxide gas substrate in the culture vessel 20.
  • First and second gas supply heads 60 and 61 which are spaced apart from each other vertically and spaced in the gas supply pipe 41 and the culture vessel 20 to supply and connected to the gas supply pipe 41, respectively.
  • first and second injection nozzles 62 and 63 connected to the gas supply heads 60 and 61.
  • the gas supply blower 40 is for supplying a carbon dioxide gas substrate to the culture vessel 20 through the gas supply pipe 41 and the gas branch pipes 43 and 44, and is installed on the gas supply pipe 41.
  • the foreign matter contained in the carbon dioxide gas is filtered by the gas filtration filter 42 to supply the optimum clean gas required in the culture conditions to the culture solution of the culture vessel 20.
  • the gas supply pipe 41 is provided with a solenoid valve 47 for opening and closing the gas supply pipe 41 with the gas filtration filter 42 back and forth.
  • the gas supply pipe 41 is installed vertically along the axial direction of the culture vessel 20, and the first and second gas distributors 43 and 44 connected to the culture vessel 20 at the lower end thereof. Branch is composed.
  • the gas branch pipes 43 and 44 are branched from the lower end of the 41 gas supply pipe () to penetrate the culture vessel 20, respectively, so that one end is inserted into the culture vessel 20, and the culture vessel 20 is provided.
  • Gas supply heads 60 and 61 having injection nozzles 62 and 63 disposed in an annular shape are respectively connected to one end inserted into each other.
  • the gas supply head (60, 61) is an annular plate-shaped pipe of the inside, it is connected and supported by the gas distributor (43, 44) inside the culture vessel 20 Located at the center of the culture vessel 20 at a certain distance from the wall surface, one or more injection nozzles (62, 63) is provided on the outer peripheral surface.
  • the injection nozzles 62 and 63 are spaced apart at regular intervals along the outer circumferential surfaces of the gas supply heads 60 and 61, and disposed in the gas supply heads 60 and 61 positioned parallel to the center of the culture vessel 20. It is installed to be inclined at a certain angle with respect.
  • the injection nozzles 62 and 63 are for controlling the injection direction of the carbon dioxide gas discharged from the gas supply heads 60 and 61. As illustrated in FIG. 4, the injection nozzles 62 and 63 are installed in the first gas supply head 60. In the case of the first injection nozzle 62 is installed downward at an angle toward the inner wall surface of the culture vessel 20, in the case of the second injection nozzle 63 installed in the second gas supply head 61 By being installed upward at a predetermined angle toward the center of the (20), it is possible to circulate and circulate the culture solution in the culture vessel 20 to be uniformly stirred.
  • the first and second injection nozzles 62 and 63 are mounted downwardly and upwardly at a predetermined angle with respect to the gas supply heads 60 and 61 so as to face each other, so that the carbon dioxide gas is injected from the injection nozzles 62 and 63.
  • the culture solution is agitated by controlling the spraying direction.
  • the carbon dioxide gas injected from the first injection nozzle 62 is downward toward the inner wall surface side of the culture vessel 20, and the carbon dioxide gas injected from the second injection nozzle 63 is upwardly directed toward the center portion of the culture vessel 20. Circulate the culture solution.
  • the stirring unit 2 sprays and supplies the carbon dioxide gas substrate to the culture vessel 20 through the injection nozzles 62 and 63 of the gas supply heads 60 and 61, and agitates the culture solution to produce the carbon dioxide gas substrate. Allow to mix evenly in the culture solution.
  • the carbon dioxide gas substrate is supplied to the injection nozzles 62 and 63 of the gas supply heads 60 and 61 through the gas supply pipe 41 and the gas branch pipes 43 and 44, and the gas branch pipes 43 and On the 44, a solenoid valve 45 for opening and closing the gas distributors 43 and 44 is provided.
  • a gas preheating heater 46 for controlling pre-heating and controlling the carbon dioxide gas substrate supplied to the culture vessel 20 through the gas filtration filter 42 at a predetermined temperature is installed.
  • the temperature control unit 3 and the heating tube 50 is installed in the form of a coil along the inner wall surface in the culture vessel 20 in order to provide the optimum temperature conditions for the culture of anaerobic microorganisms, the heating tube 50 ) Is a cooling medium supply means for supplying a refrigerant or a fruit to the fruit, and a controller 70 for controlling the operation of the electric component.
  • the heating tube 50 is to meet the effective culture conditions by maintaining the culture solution at an appropriate temperature for the cultivation of anaerobic microorganisms without changing the physical properties, the surface temperature is warmed to about 60 °C or less, the fruit inside Alternatively, the refrigerant is circulated to raise the culture solution of the culture vessel 20.
  • the cooling medium supply means connected to the heating tube 50 is a low temperature heating device for low temperature culture of the culture solution by supplying a refrigerant or a fruit to the heating tube 50, the heating tube (outside the culture vessel 20) 50 is connected between the coolant tank 53 for storing and storing the refrigerant or fruit for heat exchange with the culture solution, and is installed between the coolant tank 53 and the heating tube 50 to Cooling medium circulation pump 55 for circulating and supplying the refrigerant or the fruit to the heating pipe 50, and the low temperature culture heater 54 for heating the fruit to a predetermined temperature is installed in the cold medium tank 53 It is composed.
  • the heating tube 50 and the coolant supply means are components for maintaining low temperature culture conditions of the culture solution, and the coolant circulation pump 55 circulates the coolant or fruit according to the signal of the controller 40. do.
  • the controller 70 controls and operates each electric field component constituting the microorganism incubator 100 such as the sterilization heater 51 in addition to the cooling medium supply means, and appropriately cultures the culture solution to form an optimal culture condition. By controlling the temperature of the refrigerant and the fruit to be heated and cooled so that the culture solution is maintained at the optimum culture temperature through the heating tube (50).
  • the gas control unit 4 is for preheating and maintaining the carbon dioxide gas substrate supplied to the stirring unit 2 at an appropriate temperature to maintain the carbon dioxide gas flow rate for optimum temperature conditions of microbial culture, the gas preheating It may be configured to include a heater 46.
  • the washing sterilization unit 5 is installed in the bottom of the culture vessel 20, and after discharging the culture solution, the sterilization heater 51 for sterilizing the interior of the culture vessel 20 at a high temperature, and cleans the interior of the culture vessel 20 cleanly. It comprises a washing water spray nozzle 71, and a control solenoid valve 72 for controlling the supply of the washing water supplied to the washing water spray nozzle 71 through the washing water circulation pump (73).
  • the sterilization heater 51 is warmed to a surface temperature of about 100 °C or more, the washing water spray nozzle 71 is built in the upper part of the culture vessel 20 for the protection and extension of life of various electric and instrumentation equipment.
  • the solenoid valve 72 is configured in the pipe between the wash water circulation pump 73 and the washing water spray nozzle (71).
  • the culture solution containing the microorganism to be cultured is supplied to the culture vessel 20 via the culture solution supply pipe 30 according to the driving of the culture solution supply pump 31.
  • the inflow amount of the culture solution introduced into the culture vessel 20 can be arbitrarily confirmed through the level gauge 23 installed on the culture vessel 20 side.
  • the culture solution supply pump 31 is driven and controlled by the controller 70 which receives the level signal of the culture solution in the culture vessel 20 from the level gauge 23.
  • the controller 70 automatically stops the driving of the culture solution supply pump 31 when it is confirmed that the appropriate amount of the culture solution is filled in the culture container 20 through the level gauge 23, and the culture heater 54 is stopped.
  • the driving power is applied to heat the culture solution to a constant temperature for optimal culture.
  • the heating tube 50 is immersed in the culture solution of the culture vessel 20 to directly transfer the heat generated from the surface to the culture solution to raise the culture solution to an appropriate temperature.
  • the heating tube 50 according to the control operation of the controller 70 the culture solution at a constant temperature (about 55 ⁇ 5 °C) in the state that the surface temperature does not exceed a predetermined condition (usually 20 ⁇ 60 °C ⁇ 10 °C) Will be maintained.
  • Strains and media for culturing the microorganisms contained in the culture solution are supplied into the culture vessel 20 through the medium inlet 21 of the culture vessel 20.
  • the culture solution or the mixed solution of the culture solution and the medium is mixed and stirred with the gas substrate in the process of supplying the carbon dioxide gas substrate to the microorganism to be cultured to increase the mass transfer efficiency between the microorganism and the substrate, thereby enhancing the activation of the microorganism.
  • the carbon dioxide gas supplied through the gas supply pipe 41 is preheated to an appropriate temperature by the operation of the gas preheating heater 46 according to the signal of the controller 70. That is, the driving power is applied to the gas preheating heater 46 according to the signal of the controller 70 to drive the gas preheating heater 46, and thus the gas supply heads 60 and 61 through the gas supply pipe 41.
  • the carbon dioxide gas supplied to is preheated to an appropriate temperature and supplied.
  • the carbon dioxide gas injected through the injection nozzles 62 and 63 of the gas supply heads 60 and 61 is injected and supplied to the culture solution, and the culture solution is circulated and stirred as shown in FIG. 4.
  • the gas injected from the injection nozzles 62 and 63 passes between the edges of the first gas supply head 60 and the inner wall surface of the culture vessel 20 on both left and right sides of the culture vessel 20. It is injected downward and is circulated to rise toward the center portion of the first gas supply head 60 past the edge of the second gas supply head 61.
  • the culture solution and the medium are evenly mixed, and the carbon dioxide gas substrate injected into the culture solution can be evenly delivered to the microorganisms in the culture solution.
  • the culture solution in the culture vessel 20 is supplied with carbon dioxide gas, and agitation by carbon dioxide gas is performed without installing a separate stirrer.
  • the culture solution discharge tube 24 of the bottom of the culture vessel 20 is opened to discharge the culture solution, and the solenoid valve 72 is operated to operate the culture vessel through the washing water spray nozzle 71 ( 20) After washing the culture vessel 20 by autoclaving the washing water, the sterilization heater 51 is operated to sterilize the culture vessel 20.
  • the sterilization heater 51 is rapidly heated under a sterilization condition of about 90 ⁇ 125 ⁇ 5 °C to high temperature sterilization inside the culture vessel (20).
  • the anaerobic digestion process of the culture solution containing organic pollutants includes the following process.
  • the culture solution of the second anaerobic digester is directly introduced into the culture vessel 20 of the microorganism incubator 100 by the operation of the culture solution supply pump 31, the culture solution introduced into the culture vessel 20 as described above.
  • the medium and carbon dioxide gas substrate are mixed.
  • the microorganisms in the culture solution are grown in the dissolved carbon dioxide gas dissolved in the culture solution.
  • the culture solution in which the anaerobic microorganisms are proliferated in the microbial incubator 100 is discharged from the culture vessel 20 and returned to the second anaerobic digester, and the anaerobic microorganism is cultured using the organic contaminants of the culture solution as nutrients.
  • organic waste sludge is purified by producing digestive gas by decomposing and removing organic contaminants in the culture solution.
  • the microorganism incubator 100 of the present invention is more active in methane-producing bacteria than anaerobic hydrogen sulfide-producing bacteria during anaerobic digestion, thereby increasing methane production yield and shortening digestion time. That is, the microorganism incubator 100 can be improved by preventing the methane production yield is lowered during the anaerobic digestion to lower the methane production yield, or the methane fermentation period is lowered to lower the anaerobic digestion efficiency.
  • the microorganism incubator for inducing anaerobic digestion activity of organic waste referring to Figure 5, when the anaerobic digestion of the culture solution (or organic waste sludge) passes through the acid fermentation tank (or first anaerobic digestion tank) methane fermentation tank ( Alternatively, by being applied at the stage before the second anaerobic digestion tank), methane producing bacteria (ie, anaerobic microorganisms) useful for the methane fermentation tank are proliferated and injected, thereby enabling methane fermentation of organic waste.
  • methane producing bacteria ie, anaerobic microorganisms
  • the microbial incubator for inducing anaerobic digestion activity of organic waste improves the anaerobic digestion process used for treating and energyizing organic waste such as food waste and wastewater, livestock manure and wastewater, and sewage and wastewater sludge. It can contribute to the increase, in particular, the anaerobic microbial cultivation step in the pre-methane fermentation step of the anaerobic digestion process of the organic waste solubilized using the microbial incubator 100 to promote the anaerobic microorganisms for methane production of the culture solution It can increase the speed of digestion and increase the amount of digested gas.
  • the microbial incubator for induction of anaerobic digestion activity of the organic waste of the present invention is operated at a high temperature to perform sterilization and cultivation at the same time, to increase the biochemical reaction rate by inducing high temperature digestion in the anaerobic digestion process to increase the anaerobic digestion efficiency
  • first gas supply head 61 second gas supply head
  • washing water circulation pump 100: microorganism incubator

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Abstract

The present invention relates to a microorganism cultivator for inducing the activation of anaerobic digestion of organic waste and, more particularly, to a microorganism cultivator for inducing the activation of anaerobic digestion of organic waste wherein methane fermented microorganisms are activated in a culture solution containing organic contaminants. To this end, the present invention comprises: a hollow culture container having a culture solution supplying tube through which a culture solution enters from the outside; hollow first and second gas supply heads disposed vertically apart at a central portion within the culture container, and receiving carbon dioxide gas from a gas supply fan; a plurality of first spray nozzles connected and installed on the outer peripheral surface of the first gas supply head, and disposed at a certain angle downward toward the inner wall surface of the culture container; and a plurality of second spray nozzles connected and installed on the outer peripheral surface of the second gas supply head, and disposed at a certain angle upward toward the central portion of the culture container, wherein the gas from the first spray nozzles is sprayed downward toward the inner wall surface of the culture container, the gas from the second spray nozzles is sprayed upward toward the central portion of the culture container, and the gas is sprayed on the culture solution while the culture solution is stirred.

Description

유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기Microbial incubator for inducing anaerobic digestion activity of organic waste
본 발명은 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기에 관한 것으로, 더욱 상세하게는 유기성 오염물질을 함유한 배양용액의 메탄발효 미생물을 활성화하기 위한 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기에 관한 것이다.The present invention relates to a microorganism incubator for inducing anaerobic digestion of organic wastes, and more particularly, to a microorganism incubator for inducing anaerobic digestion of organic wastes to activate methane fermentation microorganisms of a culture solution containing organic contaminants. .

일반적으로 하수 및 폐수 슬러지 등의 유기성 폐수 또는 유기성 폐기물의 경우, 안정화를 위해 단상, 이상 소화 또는 중온 소화 등의 혐기소화 공정을 보편적으로 사용하고 있다.In general, in the case of organic wastewater or organic waste such as sewage and wastewater sludge, anaerobic digestion processes such as single phase, double digestion or mesophilic digestion are commonly used for stabilization.
그러나, 상기의 단상, 이상 소화 또는 중온 소화의 경우 20~30일 이상의 긴 체류기간이 요구되는데 비해 유기물 감량이 평균 50% 미만에 불과하여 효율적이지 못하며, 또한 소화가스의 메탄 함량이 50% 수준에 머무르고 있다.However, in the case of single-phase, abnormal digestion or mesophilic digestion, a long residence time of 20-30 days or more is required, and the organic matter loss is only less than 50% on average, and the methane content of the digestive gas is 50%. Staying.
유기성 폐기물의 혐기소화시 수리학적 체류시간이 늘어나는 요인 중 하나는, 메탄생성을 위한 혐기성 미생물이 안정되기까지의 지연기가 길고 혐기성 미생물의 활성 효율이 낮기 때문이다.One of the factors that increase the hydraulic retention time during anaerobic digestion of organic waste is the long delay time for stabilization of anaerobic microorganisms for methane production and the low efficiency of anaerobic microorganisms.
기존의 혐기소화 공정을 통해 생성된 소화가스는 이산화탄소와 메탄으로 분리되며, 메탄은 보일러의 에너지원으로 재사용되고, 이산화탄소는 압축저장하고 있다. Digestion gas produced through the existing anaerobic digestion process is separated into carbon dioxide and methane, methane is reused as the energy source of the boiler, and carbon dioxide is compressed and stored.
한편, 한국등록특허 제10-10477849호에서는 유기성 폐기물의 혐기소화 효율을 증대하기 위해 소화가스를 혐기소화조로 재순환하여 유기성 폐기물의 교반에 이용하고 있다.Meanwhile, in Korean Patent No. 10-10477849, in order to increase the anaerobic digestion efficiency of organic waste, digested gas is recycled to the anaerobic digestion tank and used for stirring the organic waste.
그러나, 상기와 같이 소화가스를 유기성 폐기물의 교반에 이용하게 되면 소화가스 내 황화수소 성분이 소화효율 저해요인으로 작용하여 혐기소화 효율이 저감되는 문제가 있다.However, when the digestion gas is used to stir the organic waste as described above, there is a problem that the hydrogen sulfide component in the digestion gas acts as a factor for inhibiting the digestion efficiency, thereby reducing the anaerobic digestion efficiency.

본 발명은 상기와 같은 점을 해결하기 위해 안출한 것으로서, 유기성 오염물질을 함유한 배양용액(혐기성 미생물을 활성화하고자 하는 용액)을 혐기성 미생물을 활성화하여 혐기소화 공정으로 공급하기 위한 것으로, 혐기성 미생물의 증식을 위한 이산화탄소 가스 기질의 공급과 동시에 배양용액의 균일한 교반이 이루어질 수 있는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 제공하는데 그 목적이 있다.The present invention has been made in order to solve the above problems, to supply the culture solution containing the organic contaminants (solution to activate the anaerobic microorganisms) by activating anaerobic microorganisms to the anaerobic digestion process, It is an object of the present invention to provide a microorganism incubator for inducing anaerobic digestion activity of organic waste, which can be uniformly stirred with a culture solution at the same time as supplying a carbon dioxide gas substrate for propagation.

상기한 목적을 달성하기 위하여 본 발명은, 외부로부터 배양용액이 유입되는 배양용액 공급관을 가지는 중공형의 배양통; 상기 배양통 내 중앙부에 상하방향으로 이격되어 위치되고, 가스공급송풍기에서 이산화탄소 가스 기질을 공급받게 되는 중공형의 제1 및 제2 가스공급헤드; 상기 제1 가스공급헤드에 외주면에 연결 설치되되, 배양통의 내벽면을 향해 일정각도로 하향 배치되는 복수의 제1 분사노즐; 상기 제2 가스공급헤드에 외주면에 연결 설치되되, 배양통의 중앙부를 향해 일정각도로 상향 배치되는 복수의 제2 분사노즐;을 포함하여 구성되며, 상기 제1 분사노즐의 가스 기질이 배양통의 내벽면 측으로 하향 분사되고 상기 제2 분사노즐의 가스 기질이 배양통의 중앙부 측으로 상향 분사되어, 배양용액에 가스 기질이 분사됨과 동시에 배양용액이 교반되도록 된 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 제공한다.In order to achieve the above object, the present invention provides a hollow culture vessel having a culture solution supply pipe into which the culture solution is introduced from the outside; Hollow first and second gas supply heads spaced vertically apart from each other in the center of the culture vessel and receiving a carbon dioxide gas substrate from a gas supply blower; A plurality of first injection nozzles connected to the outer circumferential surface of the first gas supply head and disposed downwardly at an angle toward the inner wall surface of the culture vessel; It is connected to the outer circumferential surface to the second gas supply head, a plurality of second injection nozzles are disposed upward at a predetermined angle toward the center portion of the culture vessel; and comprises a gas substrate of the first injection nozzle of the culture vessel Induction of anaerobic digestion of organic waste, characterized in that the injection downward to the inner wall surface and the gas substrate of the second injection nozzle is injected upward to the center of the culture vessel, the gas substrate is injected into the culture solution and the culture solution is stirred. It provides a microbial incubator.
바람직하게, 상기 배양통에는 배양통에 유입된 배양용액의 온도를 조절하기 위한 히팅관이 내설되고, 상기 히팅관은 배양용액 내에 잠긴 형태로 위치하게 된다.Preferably, the culture vessel has a heating tube for adjusting the temperature of the culture solution introduced into the culture vessel, the heating tube is located in a submerged form in the culture solution.
상기 히팅관에는 배양용액과의 열교환을 위한 냉매 또는 열매를 공급하는 냉열매공급수단이 연결 구성되고, 상기 냉열매공급수단은 배양통의 외측에서 히팅관에 연결되어 냉매 또는 열매를 저장 보관하는 냉열매탱크와, 냉열매탱크과 히팅관 사이에 설치되어 냉열매탱크의 냉매 또는 열매를 컨트롤러의 신호에 따라 히팅관으로 순환 공급시키기 위한 냉열매순환펌프, 및 냉열매탱크에 내설되어 열매를 일정 온도로 승온시키기 위한 배양히터로 구성된다.The heating tube is configured to be connected to a cold medium supply means for supplying a refrigerant or fruit for heat exchange with the culture solution, the cold medium supply means is connected to the heating tube from the outside of the culture vessel to store and store the refrigerant or fruit It is installed between the fruit tank, the coolant tank and the heating tube, and is installed in the coolant circulation pump for circulating and supplying the refrigerant or the fruit of the coolant tank to the heating tube according to the signal of the controller, and is installed in the coolant tank to bring the fruit to a constant temperature. It consists of a culture heater for raising the temperature.
또한 바람직하게, 상기 가스공급헤드와 가스공급송풍기 사이에는 가스공급헤드로 공급되는 이산화탄소 가스 기질을 예열하기 위한 가스예열히터가 구성된다.Also preferably, a gas preheating heater is configured between the gas supply head and the gas supply blower to preheat the carbon dioxide gas substrate supplied to the gas supply head.
또한 바람직하게, 상기 배양통에는 저부에 배양용액 배출 후 배양통 내부를 고온으로 살균하기 위한 살균히터가 내설되고, 상부에 배양통 내부를 세척하기 위한 세척수분사노즐이 내설된다.Also preferably, the culture vessel has a sterilization heater for sterilizing the inside of the culture vessel at a high temperature after discharging the culture solution at the bottom, and a washing water spray nozzle for washing the interior of the culture vessel at the top.
또한 바람직하게, 상기 배양용액 공급관에는 외부로부터 공급되는 배양용액을 공급하기 위한 배양용액 공급펌프가 연결 설치되고, 상기 배양통의 저부에는 배양하고자 한 미생물이 증식된 배양용액을 외부로 배출하기 위한 배양용액 배출관이 연결 설치된다.Also preferably, the culture solution supply pipe is connected to the culture solution supply pump for supplying the culture solution supplied from the outside, the bottom of the culture vessel culture for discharging the culture solution in which the microorganisms to be cultured to the outside The solution discharge line is connected.

본 발명에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기는, 외부로부터 메탄발효 미생물(즉, 혐기성 미생물)을 배양하고자 하는 배양용액을 직접 유입시켜 현장에서 미생물을 간편하게 배양할 수 있다.Microorganism incubator for inducing anaerobic digestion activity of organic wastes according to the present invention, by directly introducing a culture solution for culturing methane fermentation microorganisms (that is, anaerobic microorganisms) can be easily cultured microorganisms in the field.
구체적으로, 본 발명의 미생물 배양기는, 혐기성 미생물을 증식시킨 배양용액을 재순화시켜 유기성 폐기물의 처리를 위한 혐기소화 공정의 혐기소화조에 공급함으로써 유기성 폐기물의 혐기소화를 활성시킬 수 있다.Specifically, the microbial incubator of the present invention can activate the anaerobic digestion of organic wastes by recycling the culture solution in which the anaerobic microorganisms have been grown and supplying them to the anaerobic digestion tank of the anaerobic digestion process for the treatment of organic wastes.
즉, 유기성 폐기물의 혐기소화시 황화수소생성균보다 메탄생성균(혐기성 미생물)의 활성을 증진시켜 메탄생성균을 우점화시킴으로써 메탄생산 수율을 증가시킬 수 있다.That is, when anaerobic digestion of organic wastes can increase the methane production yield by increasing the activity of methane producing bacteria (anaerobic microorganisms) rather than hydrogen sulfide producing bacteria.
이에 혐기소화 공정시 메탄생성균의 활성이 낮아 메탄생산 수율이 떨어지거나, 메탄발효기간이 길어져 혐기소화 효율이 낮아지는 것을 개선할 수 있다. Therefore, the low methane production yield during the anaerobic digestion process can reduce the methane production yield, or the methane fermentation period is long, the anaerobic digestion efficiency can be improved.
그리고, 혐기소화 공정시 소화가스로부터 분리된 메탄가스는 화력, 전기 등 에너지 재생산에 이용되며, 이산화탄소 가스는 본 발명의 미생물 배양기의 가스 기질로 재사용될 수 있고, 이로써 전체 혐기소화 공정의 에너지 이용률을 증대시킬 수 있다.In addition, methane gas separated from the digestion gas during the anaerobic digestion process is used for energy reproduction such as thermal power and electricity, and carbon dioxide gas can be reused as a gas substrate of the microbial incubator of the present invention, thereby improving the energy utilization rate of the entire anaerobic digestion process. You can increase it.

도 1은 본 발명의 실시예에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 도시한 개략적인 구성도1 is a schematic configuration diagram showing a microbial incubator for inducing anaerobic digestion activity of an organic waste according to an embodiment of the present invention
도 2는 본 발명의 실시예에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 부분 도시한 구성도Figure 2 is a schematic view showing a part of the microorganism incubator for inducing anaerobic digestion of organic waste according to an embodiment of the present invention
도 3은 본 발명의 실시예에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 부분 도시한 평면도3 is a plan view partially showing a microorganism incubator for inducing anaerobic digestion activity of an organic waste according to an embodiment of the present invention
도 4는 도 2의 배양통 하부를 확대 도시한 단면도Figure 4 is an enlarged cross-sectional view of the culture vessel lower portion of FIG.
도 5는 본 발명에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기를 이용한 혐기소화 공정을 도시한 순서도5 is a flowchart illustrating an anaerobic digestion process using a microbial incubator for inducing anaerobic digestion activity of organic wastes according to the present invention.

이하, 첨부된 도면을 참조로 하여 본 발명의 바람직한 실시예를 상세하게 설명한다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.
도 1 내지 3에 도시된 바와 같이, 본 발명에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기(100)는 혐기성 미생물의 배양이 이루어지는 배양통(20)과, 배양통(20) 내에 배양시키고자 하는 배양용액(혐기성 미생물을 배양 및 증식/활성화하고자 하는 용액임, 예컨대 유기성 폐기물 등) 및 유기 배지와 무기 배지 등을 공급하기 위한 공급부(1), 배양통(20) 내에 이산화탄소 가스 기질를 공급함과 동시에 배양용액을 교반시키기 위한 교반부(2), 및 오염방지를 위한 세척살균부(5)를 포함하여 구성된다.As shown in Figures 1 to 3, the microbial incubator 100 for inducing anaerobic digestion activity of the organic wastes according to the present invention is to culture in the culture vessel 20, the culture vessel 20 is made of the culture of anaerobic microorganisms While supplying a carbon dioxide gas substrate into a supply unit 1 and a culture vessel 20 for supplying a culture solution (a solution for culturing and proliferating / activating anaerobic microorganisms, for example, organic waste, etc.) and an organic medium and an inorganic medium. It comprises a stirring part (2) for stirring the culture solution, and a washing sterilization part (5) for preventing contamination.
또한, 상기 혐기소화 활성 유도용 미생물 배양기(100)는 배양통(20) 내에 설치되어 배양용액 내의 미생물 배양을 위한 최적의 온도조건을 형성하기 위해 배양용액의 온도조건을 제어하는 온도제어부(3)와, 배양통(20) 내로 공급되는 이산화탄소 가스 기질의 온도를 적정 온도로 제어하기 위한 가스제어부(4)를 포함한다.In addition, the anaerobic digestion activity induction microorganism incubator 100 is installed in the culture vessel 20, the temperature control unit for controlling the temperature conditions of the culture solution to form the optimal temperature conditions for the culture of microorganisms in the culture solution (3) And a gas control unit 4 for controlling the temperature of the carbon dioxide gas substrate supplied into the culture vessel 20 to an appropriate temperature.
상기 배양통(20)은 중공형의 원통 수조로, 베이스프레임(미도시)에 지지되어 수직방향으로 세워지게 설치되며, 외측면에 전체적으로 폴리우레탄 등으로 된 단열재(25)가 부착 설치되고, 상부에는 균주 및 배지 등을 투입하기 위한 배지투입구(21)가 구비된다. 상기 배지투입구(21)에는 덮개(22)가 설치되어 필요시 배지투입구(21)를 개폐시킬 수 있도록 되어 있다.The culture vessel 20 is a hollow cylindrical water tank, which is supported by a base frame (not shown) and installed in a vertical direction, and is provided with a heat insulating material 25 made of polyurethane or the like on an outer surface thereof, and an upper portion thereof. The medium is provided with a medium inlet 21 for injecting the strain and the medium. The discharge inlet 21 is provided with a cover 22 to open and close the discharge inlet 21 when necessary.
그리고, 상기 배양통(20)은 투명관으로 된 레벨게이지(23)가 배양통(20)의 축선방향으로 외설되어 있어 배양통(20) 내부의 수위를 눈으로 확인할 수 있고, 이를 통해 배양통(20)의 수위를 자동 감지하여 조절할 수 있다.In addition, the culture vessel 20 is a level gauge 23 made of a transparent tube in the axial direction of the culture vessel 20 can be visually confirmed the water level inside the culture vessel 20, through this culture vessel The level of 20 can be detected and adjusted automatically.
또한, 상기 배양통(20)의 일측에는 배양용액의 온도를 확인하기 위한 온도표시계(미도시)가 설치되며, 배양통(20)의 저면에는 배양 완료된 배양용액을 배출하기 위한 배양용액 배출관(24)이 연결 설치된다.In addition, a temperature indicator (not shown) is installed on one side of the culture vessel 20 to check the temperature of the culture solution, and a culture solution discharge tube 24 for discharging the culture solution completed on the bottom of the culture vessel 20. ) Is installed connected.
상기 공급부(1)는 배양통(20) 상부로 연결되게 설치되어 배양통(20)에 배양용액을 투입하기 위한 배양용액 공급관(30)과, 외부로부터 공급되는 배양용액을 배양용액 공급관(30)에 공급하기 위한 배양용액 공급펌프(31)를 포함하여 이루어지고, 상기 배양용액 공급관(30)을 개폐하는 밸브 및 압력계 등을 포함할 수 있다.The supply unit 1 is installed to be connected to the culture vessel 20, the culture solution supply pipe 30 for injecting the culture solution into the culture vessel 20, and the culture solution supplied from the culture solution supply pipe 30 It comprises a culture solution supply pump 31 for supplying to, and may include a valve and a pressure gauge to open and close the culture solution supply pipe (30).
상기 배양용액 공급펌프(31)는 베이스프레임 상에 고정되게 설치되고, 배양용액 공급관(30)과 연결되게 설치되어 배양용액 공급관(30)을 거쳐 배양용액을 배양통(20)으로 공급한다.The culture solution supply pump 31 is fixedly installed on the base frame and is connected to the culture solution supply pipe 30 to supply the culture solution to the culture vessel 20 via the culture solution supply pipe 30.
상기 배양용액 공급펌프(31)는 미생물 배양기(100)의 외부에 위치한 폐수처리장의 폭기조 등과 배관을 통해 연결되며, 폐수처리장의 폭기액 등을 끌어올려 배양용액 공급관(30)을 통해 배양통(20) 내부로 공급하게 된다.The culture solution supply pump 31 is connected to the aeration tank of the wastewater treatment plant located outside the microorganism incubator 100 and the like, and raises the aeration solution of the wastewater treatment plant and the like through the culture solution supply pipe 30. ) Will be supplied internally.
상기 교반부(2)는 베이스프레임의 일측에 고정 설치되는 가스공급송풍기(40)와, 가스공급송풍기(40)와 배양통(20) 사이에 연결 설치되어 배양통(20) 내에 이산화탄소 가스 기질을 공급하는 가스공급배관(41), 배양통(20)에 상하로 일정 간격을 두고 이격되게 내장되고 상기 가스공급배관(41)에 연결되는 제1 및 제2 가스공급헤드(60,61), 각 가스공급헤드(60,61)에 연결 설치되는 제1 및 제2 분사노즐(62,63)을 포함하여 이루어진다.The stirring unit 2 is installed between the gas supply blower 40 and the gas supply blower 40 and the culture vessel 20 fixedly installed on one side of the base frame to provide a carbon dioxide gas substrate in the culture vessel 20. First and second gas supply heads 60 and 61 which are spaced apart from each other vertically and spaced in the gas supply pipe 41 and the culture vessel 20 to supply and connected to the gas supply pipe 41, respectively. And first and second injection nozzles 62 and 63 connected to the gas supply heads 60 and 61.
상기 가스공급송풍기(40)는 가스공급배관(41) 및 가스분기관(43,44)을 통해 배양통(20)으로 이산화탄소 가스 기질을 공급하기 위한 것으로, 가스공급배관(41) 상에 설치되어 있는 가스여과필터(42)에 의해 이산화탄소 가스에 함유되어 있는 이물질을 걸러내어 배양 조건에서 요구되는 최적의 청정가스를 배양통(20)의 배양용액으로 공급하게 된다.The gas supply blower 40 is for supplying a carbon dioxide gas substrate to the culture vessel 20 through the gas supply pipe 41 and the gas branch pipes 43 and 44, and is installed on the gas supply pipe 41. The foreign matter contained in the carbon dioxide gas is filtered by the gas filtration filter 42 to supply the optimum clean gas required in the culture conditions to the culture solution of the culture vessel 20.
상기 가스공급배관(41)에는 가스여과필터(42)를 전후로 하여 가스공급배관(41)을 개폐하기 위한 솔레노이드 밸브(47)가 설치된다.The gas supply pipe 41 is provided with a solenoid valve 47 for opening and closing the gas supply pipe 41 with the gas filtration filter 42 back and forth.
상기 가스공급배관(41)은 배양통(20)의 축선방향을 따라 수직으로 세워지게 설치되며, 그 하단부에 배양통(20)에 연결되는 제1 및 제2 가스분기관(43,44)이 분기 구성된다.The gas supply pipe 41 is installed vertically along the axial direction of the culture vessel 20, and the first and second gas distributors 43 and 44 connected to the culture vessel 20 at the lower end thereof. Branch is composed.
상기 가스분기관(43,44)은 41가스공급배관()의 하단부에서 분기되어 각각 배양통(20)을 관통하여 일단이 배양통(20)의 내부에 삽입되게 구성되며, 배양통(20)에 삽입된 일단에 각각 환형으로 배치되는 분사노즐(62,63)을 갖는 가스공급헤드(60,61)가 연결 설치된다.The gas branch pipes 43 and 44 are branched from the lower end of the 41 gas supply pipe () to penetrate the culture vessel 20, respectively, so that one end is inserted into the culture vessel 20, and the culture vessel 20 is provided. Gas supply heads 60 and 61 having injection nozzles 62 and 63 disposed in an annular shape are respectively connected to one end inserted into each other.
도 2 및 도 4에 도시된 바와 같이, 상기 가스공급헤드(60,61)는 내부가 빈 환상의 판형 파이프로, 가스분기관(43,44)에 의해 연결 지지되어 배양통(20)의 내벽면과 일정거리를 두고 배양통(20)의 중앙에 위치하게 되며, 그 외주면에 하나 이상의 분사노즐(62,63)이 구비된다.As shown in Figures 2 and 4, the gas supply head (60, 61) is an annular plate-shaped pipe of the inside, it is connected and supported by the gas distributor (43, 44) inside the culture vessel 20 Located at the center of the culture vessel 20 at a certain distance from the wall surface, one or more injection nozzles (62, 63) is provided on the outer peripheral surface.
상기 분사노즐(62,63)은 가스공급헤드(60,61)의 외주면을 따라 일정 간격으로 이격되어 배치되고, 배양통(20)의 중앙부에 평행하게 위치하고 있는 가스공급헤드(60,61)에 대해 일정 각도로 경사지게 설치된다.The injection nozzles 62 and 63 are spaced apart at regular intervals along the outer circumferential surfaces of the gas supply heads 60 and 61, and disposed in the gas supply heads 60 and 61 positioned parallel to the center of the culture vessel 20. It is installed to be inclined at a certain angle with respect.
상기 분사노즐(62,63)은 가스공급헤드(60,61)에서 배출되는 이산화탄소 가스의 분사방향을 제어하기 위한 것으로, 도 4에 도시된 바와 같이, 제1 가스공급헤드(60)에 설치되어 있는 제1 분사노즐(62)의 경우 배양통(20)의 내벽면을 향해 일정각도로 하향 설치되고, 제2 가스공급헤드(61)에 설치되어 있는 제2 분사노즐(63)의 경우 배양통(20)의 중앙부를 향해 일정각도로 상향 설치됨으로써, 배양통(20) 내 배양용액을 순환 및 회류시켜 균일하게 교반시킬 수 있다.The injection nozzles 62 and 63 are for controlling the injection direction of the carbon dioxide gas discharged from the gas supply heads 60 and 61. As illustrated in FIG. 4, the injection nozzles 62 and 63 are installed in the first gas supply head 60. In the case of the first injection nozzle 62 is installed downward at an angle toward the inner wall surface of the culture vessel 20, in the case of the second injection nozzle 63 installed in the second gas supply head 61 By being installed upward at a predetermined angle toward the center of the (20), it is possible to circulate and circulate the culture solution in the culture vessel 20 to be uniformly stirred.
상기 제1 및 제2 분사노즐(62,63)은 가스공급헤드(60,61)에 대해 일정각도로 하향 및 상향되게 장착되어 서로 대향되게 구성됨으로써 분사노즐(62,63)에서 분사되는 이산화탄소 가스의 분사방향을 제어함에 의해 배양용액을 교반시킨다.The first and second injection nozzles 62 and 63 are mounted downwardly and upwardly at a predetermined angle with respect to the gas supply heads 60 and 61 so as to face each other, so that the carbon dioxide gas is injected from the injection nozzles 62 and 63. The culture solution is agitated by controlling the spraying direction.
즉, 제1 분사노즐(62)에서 분사되는 이산화탄소 가스는 배양통(20)의 내벽면 측으로 하향되고, 제2 분사노즐(63)에서 분사되는 이산화탄소 가스는 배양통(20)의 중앙부 측으로 상향되어 배양용액을 순환시킨다.That is, the carbon dioxide gas injected from the first injection nozzle 62 is downward toward the inner wall surface side of the culture vessel 20, and the carbon dioxide gas injected from the second injection nozzle 63 is upwardly directed toward the center portion of the culture vessel 20. Circulate the culture solution.
이와 같이 상기 교반부(2)는 가스공급헤드(60,61)의 분사노즐(62,63)을 통해 이산화탄소 가스 기질을 배양통(20)에 분사 공급하는 동시에 배양용액을 교반시켜 이산화탄소 가스 기질이 배양용액 내 고르게 혼합될 수 있도록 한다.As described above, the stirring unit 2 sprays and supplies the carbon dioxide gas substrate to the culture vessel 20 through the injection nozzles 62 and 63 of the gas supply heads 60 and 61, and agitates the culture solution to produce the carbon dioxide gas substrate. Allow to mix evenly in the culture solution.
이때, 이산화탄소 가스 기질은 가스공급배관(41) 및 가스분기관(43,44)을 거쳐 가스공급헤드(60,61)의 분사노즐(62,63)로 공급되며, 상기 가스분기관(43,44) 상에는 가스분기관(43,44)의 개폐를 위한 솔레노이드 밸브(45)가 설치되어 있다.At this time, the carbon dioxide gas substrate is supplied to the injection nozzles 62 and 63 of the gas supply heads 60 and 61 through the gas supply pipe 41 and the gas branch pipes 43 and 44, and the gas branch pipes 43 and On the 44, a solenoid valve 45 for opening and closing the gas distributors 43 and 44 is provided.
그리고, 상기 가스공급배관(41) 상에는 가스여과필터(42)를 거쳐 배양통(20)으로 공급되는 이산화탄소 가스 기질을 일정온도로 미리 예열시켜 조절하기 위한 온도조절용 가스예열히터(46)가 설치된다.On the gas supply pipe 41, a gas preheating heater 46 for controlling pre-heating and controlling the carbon dioxide gas substrate supplied to the culture vessel 20 through the gas filtration filter 42 at a predetermined temperature is installed. .
한편, 상기 온도제어부(3)는 혐기성 미생물의 배양을 위한 최적의 온도조건을 제공하기 위해 배양통(20) 내에 내벽면을 따라 코일 형태로 설치되는 히팅관(50)과, 이 히팅관(50)으로 냉매 또는 열매를 공급하기 위한 냉열매공급수단, 및 전장 구성요소의 작동 제어를 위한 컨트롤러(70)를 포함하여 이루어진다.On the other hand, the temperature control unit 3 and the heating tube 50 is installed in the form of a coil along the inner wall surface in the culture vessel 20 in order to provide the optimum temperature conditions for the culture of anaerobic microorganisms, the heating tube 50 ) Is a cooling medium supply means for supplying a refrigerant or a fruit to the fruit, and a controller 70 for controlling the operation of the electric component.
상기 히팅관(50)은 배양용액을 물성변화 없이 혐기성 미생물의 배양을 위한 적정온도로 가열 유지하여 효과적인 배양조건을 충족시키기 위한 것으로, 그 표면온도가 약 60℃ 이하가 되도록 가온되고, 내부에 열매 또는 냉매가 순환 이동되어 배양통(20)의 배양용액을 승온시키게 된다.The heating tube 50 is to meet the effective culture conditions by maintaining the culture solution at an appropriate temperature for the cultivation of anaerobic microorganisms without changing the physical properties, the surface temperature is warmed to about 60 ℃ or less, the fruit inside Alternatively, the refrigerant is circulated to raise the culture solution of the culture vessel 20.
상기 히팅관(50)과 연결 구성되는 냉열매공급수단은 히팅관(50)으로 냉매 또는 열매를 공급하여 배양용액을 저온 배양시키기 위한 저온 히팅장치로서, 배양통(20)의 외측에서 히팅관(50)에 연결되어 배양용액과의 열교환을 위한 냉매 또는 열매를 저장 보관하는 냉열매탱크(53)와, 냉열매탱크(53)과 히팅관(50) 사이에 설치되어 냉열매탱크(53)의 냉매 또는 열매를 펌핑하여 히팅관(50)으로 순환 공급시키기 위한 냉열매순환펌프(55), 및 냉열매탱크(53)에 내설되어 열매를 일정 온도로 승온시키기 위한 저온용 배양히터(54)로 구성된다.The cooling medium supply means connected to the heating tube 50 is a low temperature heating device for low temperature culture of the culture solution by supplying a refrigerant or a fruit to the heating tube 50, the heating tube (outside the culture vessel 20) 50 is connected between the coolant tank 53 for storing and storing the refrigerant or fruit for heat exchange with the culture solution, and is installed between the coolant tank 53 and the heating tube 50 to Cooling medium circulation pump 55 for circulating and supplying the refrigerant or the fruit to the heating pipe 50, and the low temperature culture heater 54 for heating the fruit to a predetermined temperature is installed in the cold medium tank 53 It is composed.
여기서 상기 히팅관(50) 및 냉열매공급수단은 배양용액의 저온 배양조건을 유지하기 위한 구성요소이며, 냉열매순환펌프(55)는 상기 컨트롤러(40)의 신호에 따라 냉매 또는 열매를 순환시키게 된다.Here, the heating tube 50 and the coolant supply means are components for maintaining low temperature culture conditions of the culture solution, and the coolant circulation pump 55 circulates the coolant or fruit according to the signal of the controller 40. do.
상기 컨트롤러(70)는 냉열매공급수단 외에 살균히터(51) 등 미생물 배양기(100)를 구성하는 각 전장 구성요소를 제어하여 작동시키는 역할을 하며, 최적의 배양조건을 형성하기 위해 배양용액을 적절하게 승온 및 냉각시킬 수 있게 냉매 및 열매의 온도를 조절함으로써 히팅관(50)을 통해 배양용액을 최적의 배양온도로 유지시킨다.The controller 70 controls and operates each electric field component constituting the microorganism incubator 100 such as the sterilization heater 51 in addition to the cooling medium supply means, and appropriately cultures the culture solution to form an optimal culture condition. By controlling the temperature of the refrigerant and the fruit to be heated and cooled so that the culture solution is maintained at the optimum culture temperature through the heating tube (50).
한편, 상기 가스제어부(4)는 미생물 배양의 최적 온도조건을 위해 상기 교반부(2)로 공급되는 이산화탄소 가스 기질을 적절한 온도로 예열 및 유지하고 이산화탄소 가스 유량을 유지하기 위한 것으로, 전술한 가스예열히터(46)를 포함하여 구성될 수 있다.On the other hand, the gas control unit 4 is for preheating and maintaining the carbon dioxide gas substrate supplied to the stirring unit 2 at an appropriate temperature to maintain the carbon dioxide gas flow rate for optimum temperature conditions of microbial culture, the gas preheating It may be configured to include a heater 46.
상기 세척살균부(5)는 배양통(20) 저부에 내설되어 배양용액 배출 후 배양통(20) 내부를 고온으로 살균하기 위한 살균히터(51)와, 배양통(20) 내부를 청결하게 세척하는 세척수분사노즐(71), 및 세척수순환펌프(73)를 통해 세척수분사노즐(71)로 공급되는 세척수의 공급을 제어하기 위한 제어용 솔레노이드 밸브(72)를 포함하여 이루어진다.The washing sterilization unit 5 is installed in the bottom of the culture vessel 20, and after discharging the culture solution, the sterilization heater 51 for sterilizing the interior of the culture vessel 20 at a high temperature, and cleans the interior of the culture vessel 20 cleanly. It comprises a washing water spray nozzle 71, and a control solenoid valve 72 for controlling the supply of the washing water supplied to the washing water spray nozzle 71 through the washing water circulation pump (73).
상기 살균히터(51)는 표면온도가 약 100℃ 이상으로 가온되며, 상기 세척수분사노즐(71)은 각종 전장 및 계장기기의 보호와 수명연장을 위해 배양통(20) 상부에 내설된다.The sterilization heater 51 is warmed to a surface temperature of about 100 ℃ or more, the washing water spray nozzle 71 is built in the upper part of the culture vessel 20 for the protection and extension of life of various electric and instrumentation equipment.
상기 솔레노이드 밸브(72)는 세척수순환펌프(73)와 세척수분사노즐(71) 사이 배관에 설치 구성된다.The solenoid valve 72 is configured in the pipe between the wash water circulation pump 73 and the washing water spray nozzle (71).
상기와 같이 구성되는 본 발명의 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기(100)의 작동상태를 설명하면 다음과 같다.Referring to the operating state of the microorganism incubator 100 for inducing anaerobic digestion activity of the organic waste of the present invention configured as described above are as follows.
배양하고자 하는 미생물이 함유되어 있는 배양용액은 배양용액 공급펌프(31)의 구동에 따라 배양용액 공급관(30)을 거쳐 배양통(20)으로 공급된다.The culture solution containing the microorganism to be cultured is supplied to the culture vessel 20 via the culture solution supply pipe 30 according to the driving of the culture solution supply pump 31.
상기 배양통(20)으로 유입되는 배양용액의 유입량은 배양통(20) 측면에 설치된 레벨게이지(23)를 통해 임의로 확인할 수 있다.The inflow amount of the culture solution introduced into the culture vessel 20 can be arbitrarily confirmed through the level gauge 23 installed on the culture vessel 20 side.
상기 배양용액 공급펌프(31)는 레벨게이지(23)로부터 배양통(20) 내 배양용액의 수위 신호를 수신한 컨트롤러(70)에 의해 구동 제어된다.The culture solution supply pump 31 is driven and controlled by the controller 70 which receives the level signal of the culture solution in the culture vessel 20 from the level gauge 23.
상기 컨트롤러(70)는 레벨게이지(23)를 통해 적정량의 배양용액이 배양통(20)에 채워졌음이 확인되면 배양용액 공급펌프(31)의 구동을 자동으로 정지시키고, 배양히터(54)에 구동전원을 인가시켜 배양용액을 최적 배양을 위한 일정온도로 가열시킨다.The controller 70 automatically stops the driving of the culture solution supply pump 31 when it is confirmed that the appropriate amount of the culture solution is filled in the culture container 20 through the level gauge 23, and the culture heater 54 is stopped. The driving power is applied to heat the culture solution to a constant temperature for optimal culture.
히팅관(50)은 상기 배양통(20)의 배양용액 내에 잠기게 되어 표면에서 발생되는 열을 바로 배양용액에 전달하여 배양용액을 적정온도로 높일 수 있다.The heating tube 50 is immersed in the culture solution of the culture vessel 20 to directly transfer the heat generated from the surface to the culture solution to raise the culture solution to an appropriate temperature.
상기 히팅관(50)은 컨트롤러(70)의 제어작동에 따라 표면온도가 일정조건(통상기준 20~60℃±10℃)을 넘지 않는 상태에서 배양용액을 일정온도(약 55±5℃)로 유지시키게 된다.The heating tube 50 according to the control operation of the controller 70 the culture solution at a constant temperature (about 55 ± 5 ℃) in the state that the surface temperature does not exceed a predetermined condition (usually 20 ~ 60 ℃ ± 10 ℃) Will be maintained.
배양용액 내 함유되어 있는 미생물의 배양을 위한 균주 및 배지는 배양통(20) 상부의 배지투입구(21)를 통해 배양통(20) 내에 공급된다.Strains and media for culturing the microorganisms contained in the culture solution are supplied into the culture vessel 20 through the medium inlet 21 of the culture vessel 20.
배양용액 또는 배양용액과 배지의 혼합액은 배양하고자 하는 미생물에 이산화탄소 가스 기질이 공급되는 과정에서 가스 기질과 혼합 및 교반되어 미생물-기질 사이의 물질전달 효율이 증가되어 미생물의 활성화가 증진된다.The culture solution or the mixed solution of the culture solution and the medium is mixed and stirred with the gas substrate in the process of supplying the carbon dioxide gas substrate to the microorganism to be cultured to increase the mass transfer efficiency between the microorganism and the substrate, thereby enhancing the activation of the microorganism.
즉, 가스공급송풍기(40)가 작동되면 가스공급배관(41)을 통해 이산화탄소 가스가 공급되어 제1 및 제2 가스분기관(43,44)으로 보내지게 되고, 각 가스분기관(43,44)에 연결 설치된 가스공급헤드(60,61)로 전달 공급되어, 최종적으로 분사노즐(62,63)을 통해 배양용액에 분사되는 과정을 통해 배양용액 및 가스 기질의 혼합이 이루어지게 된다.That is, when the gas supply blower 40 is operated, carbon dioxide gas is supplied through the gas supply pipe 41 to be sent to the first and second gas branch pipes 43 and 44, and each gas branch pipe 43 and 44. ) Is supplied to the gas supply heads 60 and 61 connected to each other, and finally, the culture solution and the gas substrate are mixed through the injection nozzles 62 and 63 to the culture solution.
상기 가스공급배관(41)을 통해 공급되는 이산화탄소 가스는 컨트롤러(70)의 신호에 따른 가스예열히터(46)의 작동에 의해 적정온도로 예열되어 공급된다. 즉, 컨트롤러(70)의 신호에 따라 가스예열히터(46)에 구동전원이 인가되어 가스예열히터(46)를 구동시키게 되고, 이에 가스공급배관(41)을 통해 가스공급헤드(60,61)로 공급되는 이산화탄소 가스는 적정온도로 예열되어 공급되게 된다.The carbon dioxide gas supplied through the gas supply pipe 41 is preheated to an appropriate temperature by the operation of the gas preheating heater 46 according to the signal of the controller 70. That is, the driving power is applied to the gas preheating heater 46 according to the signal of the controller 70 to drive the gas preheating heater 46, and thus the gas supply heads 60 and 61 through the gas supply pipe 41. The carbon dioxide gas supplied to is preheated to an appropriate temperature and supplied.
상기 가스공급헤드(60,61)의 분사노즐(62,63)을 통해 분사되는 이산화탄소 가스는 배양용액으로 분사 공급됨과 동시에 도 4와 같이 배양용액을 순환 교반시키게 된다.The carbon dioxide gas injected through the injection nozzles 62 and 63 of the gas supply heads 60 and 61 is injected and supplied to the culture solution, and the culture solution is circulated and stirred as shown in FIG. 4.
도 4에 도시된 바와 같이, 상기 분사노즐(62,63)에서 분사되는 가스는 배양통(20)의 좌우 양측에서 제1 가스공급헤드(60)의 가장자리와 배양통(20)의 내벽면 사이로 하향 분사되어 제2 가스공급헤드(61)의 가장자리를 지나 제1 가스공급헤드(60)의 중앙부 측으로 상승하는 순환운동을 하게 된다. 이 과정에서 배양용액과 배지가 고르게 혼합되며, 배양용액에 분사되는 이산화탄소 가스 기질이 배양용액 내 미생물에 고르게 전달될 수 있게 된다.As shown in FIG. 4, the gas injected from the injection nozzles 62 and 63 passes between the edges of the first gas supply head 60 and the inner wall surface of the culture vessel 20 on both left and right sides of the culture vessel 20. It is injected downward and is circulated to rise toward the center portion of the first gas supply head 60 past the edge of the second gas supply head 61. In this process, the culture solution and the medium are evenly mixed, and the carbon dioxide gas substrate injected into the culture solution can be evenly delivered to the microorganisms in the culture solution.
이와 같이 상기 배양통(20) 내 배양용액은 이산화탄소 가스가 공급됨과 동시에, 별도의 교반기를 설치하지 않고서도, 이산화탄소 가스에 의한 교반이 이루어지게 된다.As described above, the culture solution in the culture vessel 20 is supplied with carbon dioxide gas, and agitation by carbon dioxide gas is performed without installing a separate stirrer.
배양용액 내 미생물의 배양이 완료되면 배양통(20) 저면의 배양용액 배출관(24)을 개방시켜 배양용액을 배출하고, 솔레노이드 밸브(72)를 작동시켜 세척수분사노즐(71)을 통해 배양통(20) 내부에 세척수를 고압분사하여 배양통(20)을 자동 세척한 후, 살균히터(51)를 가동하여 배양통(20)을 살균한다.When the culture of the microorganisms in the culture solution is completed, the culture solution discharge tube 24 of the bottom of the culture vessel 20 is opened to discharge the culture solution, and the solenoid valve 72 is operated to operate the culture vessel through the washing water spray nozzle 71 ( 20) After washing the culture vessel 20 by autoclaving the washing water, the sterilization heater 51 is operated to sterilize the culture vessel 20.
상기 살균히터(51)는 약 90~125±5℃의 살균조건으로 급속 가열되어 배양통(20) 내부를 고온 살균하게 된다.The sterilization heater 51 is rapidly heated under a sterilization condition of about 90 ~ 125 ± 5 ℃ to high temperature sterilization inside the culture vessel (20).
한편, 본 발명의 미생물 배양기(100)를 도 5와 같이 이상 이단 소화장치에 적용하는 경우 유기성 오염물질을 함유한 배양용액의 혐기소화 공정은 다음과 같은 과정을 포함하여 이루어진다.On the other hand, when the microbial incubator 100 of the present invention is applied to an abnormal two-stage digestion apparatus as shown in FIG. 5, the anaerobic digestion process of the culture solution containing organic pollutants includes the following process.
배양용액 공급펌프(31)의 작동으로 제2혐기소화조의 배양용액이 직접 미생물 배양기(100)의 배양통(20)으로 유입된 후, 전술한 바와 같이 배양통(20)에 유입된 배양용액과 배지 및 이산화탄소 가스 기질을 혼합시키게 된다.After the culture solution of the second anaerobic digester is directly introduced into the culture vessel 20 of the microorganism incubator 100 by the operation of the culture solution supply pump 31, the culture solution introduced into the culture vessel 20 as described above. The medium and carbon dioxide gas substrate are mixed.
상기 배양용액 내 미생물은 배양용액에 혼합되어 녹아드는 용존 이산화탄소 가스를 통해 증식하게 된다.The microorganisms in the culture solution are grown in the dissolved carbon dioxide gas dissolved in the culture solution.
상기 미생물 배양기(100)에서 혐기성 미생물을 증식한 배양용액은 배양통(20)에서 배출되어 상기 제2혐기소화조로 순환 복귀하게 되고, 상기 혐기성 미생물은 배양용액의 유기성 오염물질을 영양 성분으로 하여 배양되어 배양용액 내 유기성 오염물질을 분해 및 제거하여 소화가스를 생산함으로써 유기성 폐기물 슬러지를 정화시키게 된다.The culture solution in which the anaerobic microorganisms are proliferated in the microbial incubator 100 is discharged from the culture vessel 20 and returned to the second anaerobic digester, and the anaerobic microorganism is cultured using the organic contaminants of the culture solution as nutrients. Thus, organic waste sludge is purified by producing digestive gas by decomposing and removing organic contaminants in the culture solution.
이와 같이 본 발명의 미생물 배양기(100)는 혐기소화시 황화수소생성균보다 메탄생성균이 활성 증진되어 먹이 경쟁시 우점화됨으로써 결과적으로 메탄생산수율을 증가시키고 소화시간을 단축시킬 수 있게 된다. 즉, 미생물 배양기(100)는 혐기소화시 메탄생성균의 활성이 낮아 메탄생산수율이 저하되거나, 메탄발효기간이 길어져 혐기소화 효율이 낮아짐을 방지하여 개선시킬 수 있다.As described above, the microorganism incubator 100 of the present invention is more active in methane-producing bacteria than anaerobic hydrogen sulfide-producing bacteria during anaerobic digestion, thereby increasing methane production yield and shortening digestion time. That is, the microorganism incubator 100 can be improved by preventing the methane production yield is lowered during the anaerobic digestion to lower the methane production yield, or the methane fermentation period is lowered to lower the anaerobic digestion efficiency.
즉, 본 발명에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기는, 도 5를 참조하면, 혐기소화 시 배양용액(혹은 유기성 폐기물 슬러지)이 산발효조(혹은 제1혐기소화조)를 지나 메탄발효조(혹은 제2혐기소화조)로 유입되기 전 단계에 적용됨으로써 상기 메탄발효조에 유용한 메탄생성균(즉, 혐기성 미생물)을 증식 주입시키게 되며 이에 유기성 폐기물의 메탄발효를 활성화시킬 수 있게 된다.That is, the microorganism incubator for inducing anaerobic digestion activity of organic waste according to the present invention, referring to Figure 5, when the anaerobic digestion of the culture solution (or organic waste sludge) passes through the acid fermentation tank (or first anaerobic digestion tank) methane fermentation tank ( Alternatively, by being applied at the stage before the second anaerobic digestion tank), methane producing bacteria (ie, anaerobic microorganisms) useful for the methane fermentation tank are proliferated and injected, thereby enabling methane fermentation of organic waste.
이와 같이 본 발명에 따른 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기는 음식물 쓰레기와 폐수, 축산 분뇨와 폐수, 및 하수와 폐수 슬러지 등 유기성 폐기물의 처리 및 에너지화에 사용되는 혐기소화 공정을 개선하여 효율성 증대에 기여할 수 있으며, 특히 상기 미생물 배양기(100)를 이용하여 가용화 전처리된 유기성 폐기물의 혐기소화 공정 중 메탄발효 전 단계에 혐기성 미생물 배양 단계에 적용되어 메탄 생성을 위한 혐기성 미생물을 증진시켜 배양용액의 소화속도를 증가시키고, 소화가스량을 증대시킬 수 있다.As described above, the microbial incubator for inducing anaerobic digestion activity of organic waste according to the present invention improves the anaerobic digestion process used for treating and energyizing organic waste such as food waste and wastewater, livestock manure and wastewater, and sewage and wastewater sludge. It can contribute to the increase, in particular, the anaerobic microbial cultivation step in the pre-methane fermentation step of the anaerobic digestion process of the organic waste solubilized using the microbial incubator 100 to promote the anaerobic microorganisms for methane production of the culture solution It can increase the speed of digestion and increase the amount of digested gas.
또한, 본 발명의 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기는 고온으로 운전되어 살균과 배양이 동시에 이루어지고, 혐기소화 공정에서 고온 소화를 유도하여 생화학반응속도를 증가시켜 혐기소화 효율을 증가시킬 수 있으며, 배양용액 내 특정 미생물을 무오염으로 배양하여 용이하게 활성화시킬 수 있는 이점이 있다.In addition, the microbial incubator for induction of anaerobic digestion activity of the organic waste of the present invention is operated at a high temperature to perform sterilization and cultivation at the same time, to increase the biochemical reaction rate by inducing high temperature digestion in the anaerobic digestion process to increase the anaerobic digestion efficiency In addition, there is an advantage that can be easily activated by culturing specific microorganisms in a culture solution with no pollution.

[부호의 설명][Description of the code]
20 : 배양통, 21 : 배지투입구20: culture vessel, 21: medium inlet
22 : 덮개, 23 : 레벨게이지22: cover, 23: level gauge
24 : 배양용액 배출관, 25 : 단열재24: culture solution discharge pipe, 25: heat insulating material
30 : 배양용액 공급관, 31 : 배양용액 공급펌프30: culture solution supply pipe, 31: culture solution supply pump
40 : 가스공급송풍기, 41 : 가스공급배관40: gas supply blower, 41: gas supply piping
42 : 가스여과필터, 43 : 제2 가스분기관42: gas filtration filter, 43: second gas branch pipe
44 : 제1 가스분기관, 45,47,72 : 솔레노이드 밸브44: first gas distributor, 45, 47, 72: solenoid valve
46 : 가스예열히터, 50 : 히팅관46: gas preheater, 50: heating tube
51 : 살균히터, 53 : 냉열매탱크51: sterilization heater, 53: coolant tank
54 : 배양히터, 55 : 냉열매순환펌프54: culture heater, 55: cold refrigerant circulation pump
60 : 제1 가스공급헤드61 : 제2 가스공급헤드60: first gas supply head 61: second gas supply head
62 : 제1 분사노즐, 63 : 제2 분사노즐62: first injection nozzle, 63: second injection nozzle
70 : 컨트롤러, 71 : 세척수분사노즐70 controller, 71 washing water spray nozzle
73 : 세척수순환펌프, 100 : 미생물 배양기73: washing water circulation pump, 100: microorganism incubator

Claims (6)

  1. 외부로부터 배양용액이 유입되는 배양용액 공급관(30)을 가지는 중공형의 배양통(20);
    상기 배양통(20) 내 중앙부에 상하방향으로 이격되어 위치되고, 가스공급송풍기(40)에서 이산화탄소 가스 기질을 공급받게 되는 중공형의 제1 및 제2 가스공급헤드(60,61);
    상기 제1 가스공급헤드(60)에 외주면에 연결 설치되되, 배양통(20)의 내벽면을 향해 일정각도로 하향 배치되는 복수의 제1 분사노즐(62);
    상기 제2 가스공급헤드(61)에 외주면에 연결 설치되되, 배양통(20)의 중앙부를 향해 일정각도로 상향 배치되는 복수의 제2 분사노즐(63);을 포함하여 구성되며,
    상기 제1 분사노즐의 가스 기질이 배양통의 내벽면 측으로 하향 분사되고 상기 제2 분사노즐의 가스 기질이 배양통의 중앙부 측으로 상향 분사되어, 배양용액에 가스 기질이 분사됨과 동시에 배양용액이 교반되도록 된 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    A hollow culture vessel 20 having a culture solution supply pipe 30 through which the culture solution is introduced from the outside;
    Hollow first and second gas supply heads 60 and 61 spaced apart from each other in a vertical direction in the center of the culture vessel 20 to receive a carbon dioxide gas substrate from a gas supply blower 40;
    A plurality of first injection nozzles 62 connected to an outer circumferential surface of the first gas supply head 60 and disposed downwardly at an angle toward the inner wall surface of the culture vessel 20;
    A plurality of second injection nozzles 63 connected to the outer circumferential surface of the second gas supply head 61 and disposed upwardly at a predetermined angle toward the center of the culture vessel 20;
    The gas substrate of the first injection nozzle is injected downward to the inner wall side of the culture vessel and the gas substrate of the second injection nozzle is injected upward to the center portion of the culture vessel, so that the gas substrate is injected into the culture solution and the culture solution is stirred. Microorganism incubator for induction of anaerobic digestion activity of organic waste, characterized in that.

  2. 청구항 1에 있어서,
    상기 배양통(20)에는 배양통(20)에 유입된 배양용액의 온도를 조절하기 위한 히팅관(20)이 내설되고, 상기 히팅관(50)은 배양용액 내에 잠긴 형태로 위치하게 되는 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    The method according to claim 1,
    The culture tube 20 has a heating tube 20 for adjusting the temperature of the culture solution introduced into the culture tube 20, the heating tube 50 is characterized in that it is located in a submerged form in the culture solution. A microorganism incubator for inducing anaerobic digestion activity of organic waste.

  3. 청구항 1에 있어서,
    상기 가스공급헤드(60,61)와 가스공급송풍기(40) 사이에는 가스공급헤드(60,61)로 공급되는 이산화탄소 가스 기질을 예열하기 위한 가스예열히터(46)가 구성되는 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    The method according to claim 1,
    The gas preheating heater 46 is configured between the gas supply heads 60 and 61 and the gas supply blower 40 to preheat the carbon dioxide gas substrate supplied to the gas supply heads 60 and 61. Microbial incubator for inducing anaerobic digestion of waste.

  4. 청구항 1 또는 2에 있어서,
    상기 히팅관(50)에는 배양용액과의 열교환을 위한 냉매 또는 열매를 공급하는 냉열매공급수단이 연결 구성되고,
    상기 냉열매공급수단은 배양통(20)의 외측에서 히팅관(50)에 연결되어 냉매 또는 열매를 저장 보관하는 냉열매탱크(53)와, 냉열매탱크(53)과 히팅관(50) 사이에 설치되어 냉열매탱크(53)의 냉매 또는 열매를 컨트롤러(70)의 신호에 따라 히팅관(50)으로 순환 공급시키기 위한 냉열매순환펌프(55), 및 냉열매탱크(53)에 내설되고 열매를 일정 온도로 승온시키기 위한 배양히터(54)로 구성된 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    The method according to claim 1 or 2,
    The heating pipe 50 is configured to connect the cooling medium supply means for supplying a refrigerant or fruit for heat exchange with the culture solution,
    The coolant supply means is connected to the heating tube 50 on the outside of the culture vessel 20, between the coolant tank 53 for storing and storing the refrigerant or fruit, between the coolant tank 53 and the heating tube 50 Installed in the coolant circulation pump 55 for circulating and supplying the refrigerant or the fruit of the coolant tank 53 to the heating pipe 50 according to the signal of the controller 70, and is installed in the coolant tank 53. Microorganism incubator for inducing anaerobic digestion activity of organic waste, characterized in that consisting of a culture heater (54) for raising the fruit to a certain temperature.

  5. 청구항 1에 있어서,
    상기 배양통(20)에는 저부에 배양용액 배출 후 배양통(20) 내부를 고온으로 살균하기 위한 살균히터(51)가 내설되고, 상부에 배양통(20) 내부를 세척하기 위한 세척수분사노즐(71)이 내설되는 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    The method according to claim 1,
    The culture vessel 20 has a sterilization heater 51 for sterilizing the inside of the culture vessel 20 at a high temperature after discharging the culture solution at the bottom, and the washing water spray nozzle for washing the interior of the culture vessel 20 at the top ( 71) is a microbial incubator for inducing anaerobic digestion activity of organic waste, characterized in that the internal.

  6. 청구항 1에 있어서,
    상기 배양용액 공급관(30)에는 외부로부터 공급되는 배양용액을 공급하기 위한 배양용액 공급펌프(31)가 연결 설치되고, 상기 배양통(20)의 저부에는 배양하고자 한 미생물이 증식된 배양용액을 외부로 배출하기 위한 배양용액 배출관(24)이 설치되는 것을 특징으로 하는 유기성 폐기물의 혐기소화 활성 유도용 미생물 배양기.

    The method according to claim 1,
    The culture solution supply pipe 30 is connected to the culture solution supply pump 31 for supplying the culture solution supplied from the outside, the bottom of the culture vessel 20 is a culture solution in which the microorganisms to be cultured are grown outside A microbial incubator for inducing anaerobic digestion activity of organic waste, characterized in that the culture solution discharge pipe 24 for discharging to the discharge.

PCT/KR2013/001023 2012-02-08 2013-02-08 Microorganism cultivator for inducing activation of anaerobic digestion of organic waste WO2013119072A1 (en)

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