KR20130091535A - Microbe culture apparatus for inducing anaerobic digestion activity of organic wastes - Google Patents

Abstract

PURPOSE: A microbe culture apparatus for inducing anaerobic digestion activity of organic wastes is provided to facilitate cultivation of microorganism in the field by directly in-flowing a cultivation solution, in which methane fermentation microorganism is to be cultured, from outside. CONSTITUTION: A microbe culture apparatus for inducing anaerobic digestion activity of organic wastes comprises a culture barrel (20) of a hollow type having a cultivation solution supply pipe through which the cultivation solution is in-flowed from outside; a first and second gas supply heads (60,61) of a hallow type that are disposed in directions of up and down and spaced apart from each other in a center part of the culture barrel, wherein a carbon dioxide gas substrate is supplied from a gas supply fan (40) to the first and second gas supply heads; a plurality of first spray nozzles that are connectively installed on an outer surface of the first gas supply head and disposed downwardly at a predetermined angle facing an inner wall of the culture barrel; and a plurality of second spray nozzles that are connectively installed on an outer surface of the second gas supply head and disposed upwardly at a predetermined angle facing the center part of the culture barrel. When the gas substrate of the first spray nozzles is sprayed downwardly toward the inner wall of the culture barrel and the gas substrate of the second spray nozzles is upwardly sprayed toward the center part of the culture barrel, the cultivation solution is stirred while the gas substrate is sprayed to the cultivation solution.

Description

[0001] The present invention relates to a microbial culture apparatus for inducing anaerobic digestion of organic wastes,

The present invention relates to a microorganism incubator for inducing anaerobic digestion activity of organic wastes, and more particularly, to a microorganism incubator for inducing anaerobic digestion activity of organic wastes for activating methane fermentation microorganisms in a culture solution containing organic pollutants .

Generally, in the case of organic wastewater or organic waste such as sewage and wastewater sludge, an anaerobic digestion process such as single phase, abnormal digestion or mesophase digestion is commonly used for stabilization.

However, in the case of the above single phase, abnormal digestion or mesophilic digestion, a long residence time of 20 to 30 days or more is required. On the other hand, the organic matter weight loss is less than 50% on average and the methane content of the digestion gas is 50% I'm staying.

One of the factors that increase the hydraulic retention time in the anaerobic digestion of organic wastes is that the anaerobic microorganisms for methane production are long retarded and the efficiency of anaerobic microorganisms is low.

Exhaust gas generated through the conventional anaerobic digestion process is separated into carbon dioxide and methane, methane is reused as energy source of boiler, and carbon dioxide is compressed and stored.

In Korean Patent No. 10-10477849, digested gas is recycled to the anaerobic digestion tank for agitation of organic waste in order to increase anaerobic digestion efficiency of organic waste.

However, if the digested gas is used for agitation of the organic waste as described above, the hydrogen sulfide component in the extinguishing gas acts as a factor for inhibiting digestion efficiency, thereby reducing the anaerobic digestion efficiency.

Disclosure of the Invention The present invention has been made in order to solve the above problems, and an object of the present invention is to provide a culture solution (solution for activating anaerobic microorganism) containing organic pollutants to be supplied to an anaerobic digestion process by activating anaerobic microorganisms, It is an object of the present invention to provide a microorganism incubator for inducing anaerobic digestion activity of an organic waste, in which uniformly stirring the culture solution can be performed simultaneously with the supply of a carbon dioxide gas substrate for proliferation.

In order to accomplish the above object, the present invention provides a hollow culture vessel having a culture solution supply tube into which a culture solution flows from the outside; Hollow first and second gas supply heads which are vertically spaced apart from the center of the culture vessel and receive a carbon dioxide gas substrate from the gas supply blower; A plurality of first injection nozzles connected to the outer circumferential surface of the first gas supply head, the first nozzles being disposed at a predetermined angle to the inner wall surface of the culture cylinder; And a plurality of second injection nozzles connected to the outer circumferential surface of the second gas supply head and disposed upward at a predetermined angle toward the center of the culture cylinder, And the gas substrate of the second injection nozzle is injected upward toward the center of the culture tube to spray the gas substrate to the culture solution and simultaneously stir the culture solution. A microorganism culture apparatus for a microorganism.

Preferably, the culture tube contains a heating tube for controlling the temperature of the culture solution flowing into the culture tube, and the heating tube is positioned in a locked state in the culture solution.

The heating tube is connected to a cold medium supplying means for supplying a coolant or a heat for heat exchange with a culture solution, and the cold medium supplying means is connected to a heating tube outside the culture medium to cool / A cold storage circulation pump installed between the cold storage tank and the heating tube for circulating and supplying the refrigerant or the heat of the cold storage tank to the heating tube in accordance with the signal of the controller, And a culture heater for raising the temperature.

Also preferably, a gas preheating heater is provided between the gas supply head and the gas supply blower to preheat the carbon dioxide gas substrate supplied to the gas supply head.

Also, preferably, a sterilizing heater for sterilizing the inside of the culture container at a high temperature after the culture solution is drained at the bottom of the culture container, and a washing water spray nozzle for washing the inside of the culture container is installed at the top.

Preferably, a culture solution supply pump for supplying a culture solution supplied from the outside is connected to the culture solution supply pipe, and a culture solution for growing the microorganism to be cultured is discharged to the outside at the bottom of the culture bottle. A solution discharge pipe is connected.

The microorganism incubator for inducing the anaerobic digestion activity of the organic waste according to the present invention can directly inflow a culture solution for culturing methane fermenting microorganisms (that is, anaerobic microorganisms) from the outside to easily cultivate the microorganisms in the field.

Specifically, the microorganism incubator of the present invention can activate anaerobic digestion of organic waste by re-purifying the culture solution in which the anaerobic microorganism has been propagated and supplying the anaerobic digestion process to the anaerobic digestion process for the treatment of organic waste.

That is, the anaerobic digestion of organic wastes can increase methane production yield by promoting the activity of methanogenic bacteria (anaerobic microorganisms) rather than hydrogen sulfide producing bacteria, and igniting methanogenic bacteria.

Therefore, the activity of methanogenic bacteria is low during the anaerobic digestion process, so that the yield of methane production is lowered, and the methane fermentation period is prolonged, thereby reducing the anaerobic digestion efficiency.

The methane gas separated from the digestion gas during the anaerobic digestion process is used for regenerating energy such as thermal power and electricity, and the carbon dioxide gas can be reused as a gas substrate of the microbial incubator of the present invention. Thus, the energy utilization rate of the entire anaerobic digestion process Can be increased.

1 is a schematic diagram showing a microorganism incubator for inducing anaerobic digestion activity of an organic waste according to an embodiment of the present invention;
2 is a partial schematic view of a microorganism incubator for inducing anaerobic digestion activity of organic wastes according to an embodiment of the present invention
3 is a partial plan view of a microorganism incubator for inducing anaerobic digestion activity of organic wastes according to an embodiment of the present invention
Fig. 4 is a cross-sectional view of the lower portion of the culture container of Fig. 2,
5 is a flowchart showing an anaerobic digestion process using a microorganism incubator for inducing anaerobic digestion activity of organic wastes according to the present invention

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 to 3, the microorganism incubator 100 for inducing anaerobic digestion activity of the organic waste according to the present invention comprises a culture tank 20 in which anaerobic microorganisms are cultured, (1) for supplying a culture solution (a solution for culturing and proliferating / activating anaerobic microorganisms such as organic wastes, etc.), an organic medium and an inorganic medium, and the like, and supplying a carbon dioxide gas substrate An agitating part 2 for agitating the culture solution, and a cleaning sterilizing part 5 for preventing contamination.

The microorganism incubator 100 for inducing anaerobic digestion activity may further include a temperature control unit 3 installed in the culture vessel 20 for controlling a temperature condition of the culture solution to form an optimum temperature condition for culturing microorganisms in the culture solution, And a gas control unit 4 for controlling the temperature of the carbon dioxide gas substrate supplied into the culture cylinder 20 to an appropriate temperature.

The culture tube 20 is a hollow cylindrical water tank, which is supported by a base frame (not shown) so as to stand upright in a vertical direction. A heat insulating material 25 made of polyurethane or the like is adhered to the entire outer surface, A culture medium inlet 21 for supplying a strain and a medium is provided. A lid 22 is provided at the discharge port 21 so that the discharge port 21 can be opened or closed when necessary.

The leveling gauge 23 made of a transparent tube is pierced in the axial direction of the cultivation bucket 20 so that the level of the inside of the culture bucket 20 can be visually confirmed. It is possible to automatically detect and adjust the water level of the water tank 20.

A temperature indicator (not shown) for confirming the temperature of the culture solution is provided at one side of the culture tube 20 and a culture solution discharge tube 24 for discharging the cultured solution at the bottom of the culture tube 20 ).

The supplying unit 1 is connected to the upper part of the culture tank 20 and includes a culture solution supply pipe 30 for supplying the culture solution to the culture tank 20, a culture solution supply pipe 30 for supplying the culture solution supplied from the outside, And a valve and a pressure gauge for opening and closing the culture solution supply pipe 30, and the like.

The culture solution supply pump 31 is installed on the base frame and is connected to the culture solution supply pipe 30 and supplies the culture solution to the culture solution bottle 20 via the culture solution supply pipe 30.

The culture solution supply pump 31 is connected to the aeration tank of the wastewater treatment plant located outside the microorganism incubator 100 through a pipe to raise the aeration solution in the wastewater treatment plant, ).

The agitating unit 2 includes a gas supply blower 40 fixed to one side of the base frame and a gas supply blower 40 connected between the gas supply blower 40 and the cultivation cistern 20 to supply a carbon dioxide gas substrate The gas supply piping 41 and the first and second gas supply heads 60 and 61 are vertically spaced apart from each other at a predetermined interval and connected to the gas supply pipe 41, And first and second injection nozzles (62, 63) connected to the gas supply heads (60, 61).

The gas supply blower 40 is for supplying a carbon dioxide gas substrate to the culture container 20 through the gas supply pipe 41 and the gas branch pipes 43 and 44 and is provided on the gas supply pipe 41 Which is contained in the carbon dioxide gas is filtered by the gas filtration filter 42 to supply the optimum clean gas required under the culture conditions to the culture solution in the culture tank 20. [

The gas supply pipe 41 is provided with a solenoid valve 47 for opening and closing the gas supply pipe 41 with the gas filtration filter 42 therebetween.

The gas supply pipe 41 is vertically installed along the axial direction of the culture bottle 20 and has first and second gas branch pipes 43 and 44 connected to the culture bottle 20 at a lower end thereof Branch.

The gas branch pipes 43 and 44 are branched from the lower end of the gas supply pipe 41 and are respectively inserted through the culture container 20 and one end thereof inserted into the culture container 20, And gas supply heads 60 and 61 having injection nozzles 62 and 63 arranged annularly at one end thereof inserted into the gas supply heads 60 and 61, respectively.

As shown in FIGS. 2 and 4, the gas supply heads 60 and 61 are plate-shaped pipes having an inner hollow shape and are connected and supported by gas branch pipes 43 and 44, And is located at the center of the culture bin 20 with a certain distance from the wall surface, and one or more injection nozzles 62 and 63 are provided on the outer peripheral surface thereof.

The injection nozzles 62 and 63 are spaced apart from each other along the outer circumferential surface of the gas supply heads 60 and 61 and are arranged in parallel to the gas supply heads 60 and 61 At a predetermined angle.

The injection nozzles 62 and 63 are provided for controlling the injection direction of the carbon dioxide gas discharged from the gas supply heads 60 and 61 and are provided in the first gas supply head 60 And the second injection nozzle 63 provided at the second gas supply head 61 is installed at a lower position toward the inner wall surface of the culture cylinder 20 in the case of the first injection nozzle 62 provided in the culture tank 20. [ The culture solution in the culture tube 20 can be circulated and stirred to be uniformly stirred by being installed upward at a predetermined angle toward the center of the culture tube 20.

The first and second injection nozzles 62 and 63 are mounted on the gas supply heads 60 and 61 so as to be downwardly and upwardly opposed to each other at an angle to be opposed to each other, And the culture solution is stirred by controlling the spraying direction of the culture solution.

That is, the carbon dioxide gas injected from the first injection nozzle 62 is directed downward toward the inner wall surface of the culture cylinder 20, and the carbon dioxide gas injected from the second injection nozzle 63 is upwardly directed toward the center of the culture cylinder 20 The culture solution is circulated.

The agitator 2 injects the carbon dioxide gas into the culture vessel 20 through the injection nozzles 62 and 63 of the gas supply heads 60 and 61 and simultaneously stirs the culture solution, So that they can be mixed evenly in the culture solution.

At this time, the carbon dioxide gas substrate is supplied to the injection nozzles 62 and 63 of the gas supply heads 60 and 61 through the gas supply pipe 41 and the gas branch pipes 43 and 44, 44, a solenoid valve 45 for opening and closing the gas branch pipes 43, 44 is provided.

A temperature control gas preheater 46 is provided on the gas supply pipe 41 to preheat the carbon dioxide gas supplied to the culture container 20 through the gas filtering filter 42 to a predetermined temperature .

The temperature control unit 3 includes a heating tube 50 installed in the culture vessel 20 along the inner wall surface in the form of a coil to provide an optimal temperature condition for culturing the anaerobic microorganism, And a controller 70 for controlling the operation of the electric field component.

The heating tube 50 is heated to maintain the culture solution at an appropriate temperature for culturing the anaerobic microorganism without changing physical properties to satisfy effective culture conditions. The heating tube 50 is heated to have a surface temperature of about 60 캜 or less, Or the refrigerant circulates and the temperature of the culture solution in the culture tank 20 is raised.

The cold water supplying means connected to the heating pipe 50 is a low temperature heating device for supplying a refrigerant or a heat to the heating pipe 50 to cultivate the culture solution at a low temperature, 50 for storing the refrigerant or the fruit for heat exchange with the culture solution and a cold storage tank 53 provided between the cold storage tank 53 and the heating tube 50 for storing the refrigerant or the heat for the heat exchange with the cold storage tank 53 A circulation pump 55 for circulating a coolant or a pumped material to the heating pipe 50 and a low temperature culture heater 54 for heating the fruit to a predetermined temperature, .

Here, the heating pipe 50 and the cold liquor supply unit are components for maintaining the low temperature culture condition of the culture solution, and the cold liquor circulation pump 55 circulates the refrigerant or the liquor according to the signal of the controller 40 do.

The controller 70 serves to control and operate the electric field components constituting the microorganism incubator 100 such as the sterilizing heater 51 in addition to the cold and hot water supplying means and to operate the culture solution appropriately The temperature of the refrigerant and the fruit is controlled so that the temperature of the culture solution can be raised and cooled.

Meanwhile, the gas control unit 4 is for preheating and maintaining the carbon dioxide gas substrate supplied to the agitating unit 2 to an appropriate temperature for maintaining the optimum temperature condition of the microbial culture, and to maintain the carbon dioxide gas flow rate. And a heater 46 as shown in FIG.

The washing and sterilizing unit 5 includes a sterilizing heater 51 disposed at the bottom of the culture tank 20 for sterilizing the inside of the culture tank 20 at a high temperature after discharging the culture solution, And a control solenoid valve 72 for controlling the supply of the wash water supplied to the wash water spray nozzle 71 through the wash water circulation pump 73.

The sterilizing heater 51 is heated to a surface temperature of about 100 ° C or more. The washing water spraying nozzle 71 is placed in the upper part of the culture tank 20 to protect various electric and instrumentation devices and extend their service life.

The solenoid valve 72 is installed in a pipe between the washing water circulation pump 73 and the washing water spray nozzle 71.

Hereinafter, the operation of the microorganism incubator 100 for inducing anaerobic digestion activity of the organic waste of the present invention will be described.

The culture solution containing the microorganism to be cultivated is supplied to the culture tank 20 through the culture solution supply pipe 30 according to the driving of the culture solution supply pump 31.

The inflow amount of the culture solution flowing into the cultivation trough 20 can be arbitrarily confirmed through the level gauge 23 provided on the side surface of the culture trough 20.

The culture solution supply pump 31 is driven and controlled by the controller 70 which receives the level signal of the culture solution in the culture tank 20 from the level gauge 23.

The controller 70 automatically stops the driving of the culture solution supply pump 31 when it is confirmed that a proper amount of the culture solution is filled in the culture bottle 20 through the level gauge 23, The driving power is applied to heat the culture solution to a constant temperature for optimal cultivation.

The heating tube 50 is immersed in the culture solution in the culture tube 20, and heat generated on the surface is directly transferred to the culture solution to raise the temperature of the culture solution to an appropriate temperature.

The heating tube 50 is heated to a predetermined temperature (about 55 ± 5 ° C) under the condition that the surface temperature does not exceed a certain condition (normally 20 to 60 ° C ± 10 ° C) in accordance with the control operation of the controller 70 .

The strain and the culture medium for culturing the microorganisms contained in the culture solution are supplied into the culture cuvette 20 through the culture medium inlet 20 at the upper part of the culture cuvette 20.

The culture solution or the mixture of the culture solution and the medium is mixed with the gas substrate in the process of supplying the carbon dioxide gas substrate to the microorganism to be cultured, and the efficiency of mass transfer between the microorganisms and the substrate is increased to enhance the activation of the microorganism.

That is, when the gas supply blower 40 is operated, carbon dioxide gas is supplied through the gas supply pipe 41 to be sent to the first and second gas branch pipes 43 and 44, And then finally injected into the culture solution through the injection nozzles 62 and 63 to mix the culture solution and the gas substrate.

The carbon dioxide gas supplied through the gas supply pipe 41 is preheated and supplied to an appropriate temperature by the operation of the gas preheating heater 46 according to the signal from the controller 70. That is, the driving power is applied to the gas preheating heater 46 in accordance with the signal of the controller 70 to drive the gas preheating heater 46, and the gas supply heads 60 and 61 are driven through the gas supply pipe 41, The carbon dioxide gas to be supplied to the reactor is preheated and supplied to an appropriate temperature.

The carbon dioxide gas injected through the injection nozzles 62 and 63 of the gas supply heads 60 and 61 is injected and supplied into the culture solution, and the culture solution is circulated and agitated as shown in FIG.

4, the gas injected from the injection nozzles 62 and 63 flows between the edge of the first gas supply head 60 and the inner wall surface of the culture container 20 on both left and right sides of the culture container 20 The gas is supplied to the first gas supply head 60 through the edge of the second gas supply head 61 and moves upward toward the center of the first gas supply head 60. In this process, the culture solution and the culture medium are evenly mixed, and the carbon dioxide gas substrate injected into the culture solution can be uniformly transferred to the microorganisms in the culture solution.

Thus, the culture solution in the cultivation cylinder 20 is supplied with the carbon dioxide gas and stirred by the carbon dioxide gas without providing a separate stirrer.

When the culture of the microorganism in the culture solution is completed, the culture solution discharge pipe 24 at the bottom of the culture bottle 20 is opened to discharge the culture solution, and the solenoid valve 72 is operated to discharge the culture solution through the wash water injection nozzle 71 20, the culture tank 20 is automatically cleaned by injecting washing water under high pressure, and then the sterilizing heater 51 is operated to sterilize the culture tank 20. [

The sterilization heater 51 is rapidly heated under sterilization conditions of about 90 to 125 5 C to sterilize the inside of the culture cans 20.

Meanwhile, when the microorganism incubator 100 of the present invention is applied to an abnormal two-stage extinguishing apparatus as shown in FIG. 5, the anaerobic digestion process of the culture solution containing organic pollutants includes the following process.

The culture solution of the second anaerobic digestion tank is directly introduced into the culture tank 20 of the microorganism culture apparatus 100 by the operation of the culture solution supply pump 31 and then the culture solution introduced into the culture tank 20 The medium and the carbon dioxide gas substrate are mixed.

The microorganisms in the culture solution are mixed with the culture solution and dissolved by the dissolved carbon dioxide gas.

The culture solution in which the anaerobic microorganism is propagated in the microorganism incubator 100 is discharged from the culture tank 20 and circulated back to the second anaerobic digestion tank. The anaerobic microorganism is cultivated using the organic contaminants of the culture solution as nutrients Thereby decomposing and removing organic pollutants in the culture solution to produce digested gas, thereby purifying the organic waste sludge.

As described above, the microorganism incubator 100 according to the present invention promotes the activity of methanogenic bacteria more than the hydrogen sulfide-producing microorganisms during anaerobic digestion, and is ignited when the food is competing. As a result, the yield of methane production can be increased and the digestion time can be shortened. That is, the microorganism incubator 100 can prevent the lowering of the yield of methane production due to the low activity of methanogens during anaerobic digestion and the lowering of the anaerobic digestion efficiency due to the longer methane fermentation period.

5, the culture solution (or the organic waste sludge) during anaerobic digestion passes through an acid fermentation tank (or a first anaerobic digestion tank), and a methane fermentation tank Or the second anaerobic digestion tank), the methane-producing bacteria (that is, anaerobic microorganisms) useful for the methane fermentation tank are injected and the methane fermentation of the organic waste can be activated.

As described above, the microorganism incubator for inducing the anaerobic digestion activity of organic wastes according to the present invention improves the anaerobic digestion process used for treating and energizing organic wastes such as food waste, wastewater, livestock manure and wastewater, and sewage and wastewater sludge, In particular, in the anaerobic digestion process of the organic wastes pretreated by using the microorganism incubator 100, the anaerobic microorganism is applied to the anaerobic microorganism culture stage before the methane fermentation stage to enhance anaerobic microorganisms for methane production, The digestion rate can be increased and the digestion gas amount can be increased.

In addition, the microorganism incubator for inducing anaerobic digestion activity of organic wastes of the present invention is operated at a high temperature to perform sterilization and cultivation at the same time, induces high temperature digestion in the anaerobic digestion process, thereby increasing the biochemical reaction rate to increase anaerobic digestion efficiency And there is an advantage that the specific microorganism in the culture solution can be easily activated by culturing it as uncontaminated.

20: culture tube 21:
22: cover 23: level gauge
24: Culture solution discharge pipe 25: Insulation
30: culture solution supply pipe 31: culture solution supply pump
40: gas supply blower 41: gas supply pipe
42: gas filtration filter 43: second gas distribution tube
44: first gas distributor 45, 47, 72: solenoid valve
46: gas preheating heater 50: heating pipe
51: Sterilization heater 53: Cold storage tank
54: incubator heater 55: cold and cold circulation pump
60: first gas supply head 61: second gas supply head
62: first injection nozzle 63: second injection nozzle
70: controller 71: wash water spray nozzle
73: wash water circulation pump 100: microorganism incubator

Claims (6)

A hollow culture container 20 having a culture solution supply pipe 30 into which a culture solution flows from the outside;
First and second hollow gas supply heads (60, 61) spaced apart from each other in the vertical direction in the culture chamber (20) and receiving a carbon dioxide gas substrate from the gas supply blower (40);
A plurality of first injection nozzles (62) connected to the outer circumferential surface of the first gas supply head (60), the first spray nozzles (62) being disposed at a predetermined angle to the inner wall surface of the culture tank (20);
And a plurality of second injection nozzles (63) connected to the outer circumferential surface of the second gas supply head (61) and disposed upward at a predetermined angle toward a central portion of the cultivation cylinder (20)
The gas substrate of the first injection nozzle is sprayed downward to the inner wall surface side of the culture cylinder and the gas substrate of the second injection nozzle is injected upward to the central portion side of the culture cylinder so that the gas substrate is injected into the culture solution and the culture solution is stirred Wherein the anaerobic digestion activity of the organic waste is determined by the microorganism.
The method according to claim 1,
The culture tube 20 has a heating tube 20 for adjusting the temperature of the culture solution introduced into the culture tube 20, the heating tube 50 is characterized in that it is located in a submerged form in the culture solution. A microorganism incubator for inducing anaerobic digestion activity of organic waste.
The method according to claim 1,
Characterized in that a gas preheater (46) for preheating a carbon dioxide gas substrate supplied to the gas supply heads (60, 61) is formed between the gas supply heads (60, 61) and the gas supply blower (40) Microbiological incubator for inducing anaerobic digestion activity of wastes.
The method according to claim 1 or 2,
The heating pipe (50) is connected to a coolant supplying means for supplying a coolant or a heat for heat exchange with a culture solution,
The hot and cold supply means includes a cold storage tank 53 connected to the heating tube 50 at the outer side of the culture cylinder 20 for storing and storing the refrigerant or the fruit, (55) for circulating the coolant or the heat of the cold storage tank (53) to the heating pipe (50) in accordance with a signal from the controller (70), and a cold storage tank And a culture heater (54) for heating the fruit to a predetermined temperature. The microorganism incubator for inducing anaerobic digestion activity of organic wastes.
The method according to claim 1,
A sterilizing heater 51 for sterilizing the inside of the cultivation cistern 20 at a high temperature after the culture solution is drained at the bottom of the culture cistern 20 and a washing water spray nozzle for washing the inside of the culture cistern 20 71) is introduced into the anaerobic digestion reactor.
The method according to claim 1,
A culture solution supply pump 31 for supplying a culture solution supplied from the outside is connected to the culture solution supply pipe 30. A culture solution in which the microorganisms to be cultured are grown, And a culture solution discharge pipe (24) for discharging the organic solution to the anaerobic digestion activity of the microorganism.

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