KR101290246B1 - Microbial fermentor multistage jacket installed - Google Patents

Abstract

PURPOSE: A microbial culturing machine with a multistage jacket is provided to control a supply of a heating medium which is supplied to a plurality of unit jackets, thereby culturing without a heat-denatured medium when the quantity of a medium is less than 70%. CONSTITUTION: A microbial culturing machine with a multistage jacket comprises the jacket for controlling temperature at the outside of a reaction tank with a stirring device. The microbial culturing machine comprises the jacket which has multistage unit jackets (30a, 30b, 30c) with an inlet (31) and an outlet (32) for a heating medium and differently controls temperature in a vertical direction of the reaction tank; a first temperature control device (40) which controls temperature by selectively supplying each heating medium to the unit jackets; and a second temperature control device (50) with a screw wing (51) for controlling temperature at a rotary shaft (21) of the stirring device (20). The microbial culturing machine is able to control temperature in a vertical direction according to the quantity of a medium in the reaction tank. [Reference numerals] (70) Control unit; (AA) Y axis; (BB,CC,FF) Steam; (DD,EE,GG) Cooling water

Description

Microbial fermentor multistage jacket installed}

The present invention relates to a microbial incubator equipped with a multi-stage jacket configured to suppress the reduction of heat loss and the occurrence of thermal degeneration of the medium. More specifically, the incubator is provided with a jacket consisting of a multi-stage unit jacket that can be operated separately divided. It is possible to adjust the size (height) of the jacket for temperature control and sterilization according to the culture amount of the incubator while controlling the incubation volume of the incubator. It relates to a microbial incubator with a multi-stage jacket made to maximize culture efficiency by minimizing thermal degeneration of the medium due to overheating.

In general, a fermentor (fermentor) is made to artificially grow bacteria or microorganisms, and before use, it is necessary to sterilize the inside of the reaction tank to prevent the occurrence of various germs, and adjust the pH or temperature according to the use.

As shown in FIG. 1, a conventional incubator includes a reaction tank 1 for accommodating a medium (culture medium) and cultures bacteria or microorganisms, and a stirring blade installed in the reaction tank 1 to stir the medium and a motor for rotating the medium. A device (2), a single type jacket (3) installed on the outside of the reaction tank to adjust the temperature of the reaction tank (1), and the temperature to control the temperature by supplying steam or cooling water to the heating medium to the jacket (3) It consists of an adjusting device (4).

Sterilization of the reaction tank (1) is sterilized by supplying high-temperature steam, this technique is provided with a variety of techniques, such as indirect sterilization microorganism incubator of the conventional (KR) Patent Registration No. 10-0821376.

However, in the case of using the jacket 3 of the single type as shown in FIG. 2, when the medium accommodated and cultured in the reactor 1 does not reach the proper culture capacity of the reactor 1, as shown in FIG. There is a disadvantage of causing thermal denaturation in the medium.

For example, a typical culture volume (Vol%) is 70-80% of the volume of the reactor, and when the temperature is controlled using a single type jacket (3), the amount of medium is 70-80% of the volume of the reactor. When the volume of air layer is small, and the jacket is not wrapped in the upper part, there is no big temperature deviation between the inner wall of the reactor layer and the inner wall of the air layer in contact with the air. And thermal degeneration of the medium does not occur in the inner wall of the air layer.

However, when the amount of the medium is 50% or 30% of the volume of the reaction tank as shown in Fig. 2 (a) or (b), the volume of the air layer is large, and the jacket surrounds the outer side of the reaction tank forming the air layer. In the inner wall, a large temperature deviation occurs between the inner wall of the medium layer (C) in contact with the medium and the inner wall of the air layer (B) in contact with the air, and as a result, the boundary between the medium and the air layer and the inner wall of the air layer (1b) as shown in FIG. 3. Thermal degeneration of the medium occurs, and there is a problem that the thermal efficiency is lowered by transferring heat to the air layer (B) as well as the medium layer (C).

In more detail, when a small amount of medium is used without putting a medium of a medium in a reactor equipped with a single jacket 3, the inner layer of the medium layer C is formed by a heat medium supplied to the jacket 3. A temperature deviation occurs between (1c) and the inner wall 1b of the air layer B, while the inner wall 1b of the air layer B is heated to a higher temperature than the inner wall 1c of the discharge layer C, so that the boundary between the medium and the air layer is There is a problem that thermal degeneration occurs in the medium with the air layer (B) inner wall (1b) due to the frying phenomenon when the medium and the stirring, and also to control the temperature by heating only the inner wall (1c) of the medium layer (C) for cultivation It is heated to the same as the inner wall (1b) of the air layer (B) there is a problem that the thermal efficiency is lowered.

In addition, there is no method to prevent thermal denaturation as described above, so that the culture medium is always put in the appropriate capacity in the reaction tank, so that the elasticity of the culture capacity is low, and the efficiency of the culture is low. In the case of cultivation, a small incubator is purchased separately, or cultivation of a larger amount of medium than that of the medium to be cultured has a problem of causing a decrease in efficiency and a rise in price thereof.

(KR) Registered Patent Publication No. 10-0425779 (KR) Registered Patent Publication No. 10-0821376

The present invention is to solve the problem of the conventional incubator with a single type of jacket, the present invention can be selected regardless of the size of the volume of the reactor, the amount of the medium to be cultured, The present invention is to provide a microorganism incubator with a multi-stage jacket that is configured to suppress the generation of thermally denatured medium and improve thermal efficiency.

That is, a multi-stage jacket is provided having a jacket composed of a plurality of multi-stage unit jackets, each of which can be operated by being partitioned to adjust the heating height of the reactor according to the amount of the medium to control the medium culture capacity. To provide a microbial incubator.

In addition, in the conventional case, there is a problem that the nonuniformity of the temperature of the inside and the outside occurs due to the heating by the heat transmitted from the jacket provided on the outside of the reaction tank, a temperature control device is provided on the stirring shaft to solve this problem To, but to provide a microbial incubator is installed a multi-stage jacket made so that the temperature control device can be applied according to the culture medium capacity.

Microorganism incubator equipped with a multi-stage jacket of the present invention to achieve the above object, consisting of a multi-stage unit jacket is provided with an inlet and an outlet for the heating medium to surround the reaction tank to be divided into a plurality of vertical direction of the reaction tank temperature in the vertical direction of the reaction tank The first temperature control device for controlling the temperature by selectively supplying the heat medium to the unit jacket and the unit jacket, respectively, to control the temperature differently, and the stirring shaft of the stirring device is installed in the form of a screw on the outer periphery of the medium Including a second temperature control device made of a temperature control screw wings that can be moved in the upper or lower direction according to the rotation direction, characterized in that the temperature can be controlled to be partitioned in the vertical direction according to the amount of medium in the reactor.

The temperature control screw blade is made of a heat-transfer material that is heated by receiving electricity, while the means for preventing the medium from rising continuously in the vertical direction by taking the temperature control screw blade is a screw blade for temperature control at random intervals on the stirring shaft Characterized in that it is provided with a rise prevention portion is not installed.

The temperature control device is characterized in that the temperature of the heating medium supplied to the unit jacket is divided into a plurality of temperature can be adjusted according to the height of the reaction tank.

The microbial incubator provided with the multi-stage jacket of the present invention made as described above is provided with a jacket consisting of a multi-stage unit jacket divided into a plurality of horizontally in the reaction tank, it is possible to control the supply of the heat medium supplied to the plurality of unit jackets Even if the amount of the medium accommodated in the reactor is 70% or less, such as 30 to 50% of the volume of the reactor, it is possible to incubate without the generation of a thermally denatured medium, and the thermal efficiency of the reactor by not heating the tank more than necessary. There is an advantage that can prevent the degradation.

In addition, by using a heating device to control the supply temperature of each heat medium supplied to a plurality of unit jackets more easily and can be adjusted differently, the culture temperature can be changed depending on the height of the reaction tank There is an advantage to improve the thermal efficiency at the same time.

1 is a schematic configuration diagram of a culture apparatus equipped with a conventional single type jacket.
Figure 2 is a schematic cross-sectional view of a conventional incubator with a single type of jacket is a schematic view when the medium to be cultured to 50% or 30% of the reactor volume.
3 is a detailed schematic view of the portion A of FIG.
Figure 4 is a partial perspective schematic view of a microbial incubator with a multi-stage jacket showing one embodiment of the present invention.
Figure 5 is a schematic diagram of a microbial incubator having a multi-stage jacket is shown showing an embodiment of the present invention.
Figure 6 is a schematic diagram of a rotating shaft for stirring a second temperature control device is installed microorganism incubator of the present invention multi-stage jacket.
Figure 7 is a partial detailed schematic view of the rotating shaft for agitation is installed a second temperature control device of the microbial incubator with the multi-stage jacket of the present invention.
8 is a schematic configuration diagram of a microbial incubator having a multi-stage jacket showing another embodiment of the present invention.

It is to be understood that the terms and words used in the present specification and claims should not be construed as limited to a conventional or dictionary sense and that the configurations shown in the embodiments and drawings described herein are only the most preferred embodiments of the present invention It is to be understood that the invention is not limited to the specific embodiments disclosed herein, and that various equivalents and modifications may be made thereto without departing from the scope of the present invention.

The present invention relates to an incubator for artificially cultivating (growing) bacteria or microorganisms, and to a microbial incubator equipped with a multi-stage jacket, which allows to arbitrarily select an amount of medium to be cultured without being limited by the volume of the reactor's reactor. .

The microorganism incubator with the multistage jacket of the present invention will be described with reference to the accompanying drawings.

4 and 5,

Microorganism incubator equipped with a multi-stage jacket of the present invention, a jacket for temperature control is formed to surround the reaction tank 10, the stirring device 20 for stirring the medium in the reaction tank 10, and the outer peripheral surface of the reaction tank 10 30 and the first temperature control device 40 for supplying a heat medium to the jacket 30 to transfer heat from the outside of the reaction tank 10 to adjust the temperature, and the stirring shaft of the stirring device 20 The second temperature control device 50 is installed in the (21) to transfer heat from the inside of the reaction tank 10 to adjust the temperature, the heat insulating material 60 surrounding the jacket 30, and the medium of the reaction tank 10 It consists of a control unit 70 for controlling the culture and sterilization.

The reactor 10 is a conventional one, and is provided with an air supply pipe 11 for supplying air (Air) to the inner lower end of the reactor 10, and a drain pipe 12 at the lower end.

The air supply pipe 11 is provided with a check valve (C.V) for preventing the back flow, the steam supply pipe is installed is installed valve (V) to supply steam for sterilization treatment.

Two valves V are installed in the drain pipe 12, and a steam supply pipe in which the valve V is installed to supply steam for sterilization is connected between the valves V. Since the steam supply pipe is connected between the two valves (V) it is possible to discharge the material in the reaction tank 10 at the time of discharging, it is possible to supply steam through the drain pipe 12 in the reaction tank 10 during sterilization treatment.

The reactor 10 is provided with a level gauge (not shown) and a temperature sensor T to check the water level and temperature, the temperature sensor (T) is electrically connected to the control unit 70 of the reactor 10 Provide information. The temperature sensor (T) is preferably a plurality of temperature sensors (Ta, Tb, Tc) is installed in the vertical direction of the reaction tank 10 to measure the temperature according to the height of the reaction tank (10). That is, the controller 70 selectively selects a jacket 30 including a plurality of unit jackets 30a, 30b, and 30c formed in multiple stages, which are formed by using information transmitted from a plurality of temperature sensors Ta, Tb, and Tc. It may be used as, or to adjust the supply temperature of the heat medium, and supplies power to the second temperature control device (50).

The stirring device 20 is to agitate the medium in the reaction tank 10 to facilitate the metabolic activity of the microorganisms or bacteria to be cultured, the rotating shaft 21 for the stirring installed in the central vertical axis (Y axis) of the reaction tank 10 and , A plurality of stirring blades 22 installed to be spaced apart in the vertical direction on the stirring rotating shaft 21, and a motor M for rotating the stirring rotating shaft 21 provided with the stirring blade 21. Although the number of the plurality of stirring blades 22 is not limited, it is usually preferred that two to five are provided at regular intervals in the vertical direction.

A second temperature control device 50 is installed on the stirring shaft 21 as a heat source for controlling temperature, and FIGS. 5 to 8 are attached to the second temperature control device 50. It will be described in detail.

The second temperature control device 50 is configured to adjust the temperature in the reactor 10 together with the first temperature control device 40 or the first temperature control device to supply heat from the outside of the reactor 10 ( Unlike the second 40, the second temperature control device 50 is configured to supply heat from the inside of the reactor (10).

The second temperature control device 50 as the heat source, the first temperature control screw blades 51a which are spaced apart in a screw form on the outer periphery of the rotating shaft 21 for stirring as shown in FIGS. 6 and 7. ), The second temperature control screw blade (51b), the third temperature control screw blade (51c), such as a plurality of temperature control screw blades (51), the first temperature control screw blade (51a), for the second temperature control The screw blade 51b and the third temperature control screw blade 51c are spaced apart from each other and are provided with a plurality of climb prevention parts such as the first rise prevention part 52a and the second rise prevention part 52b which are generated between the parts. It is made of a portion 52, the temperature control screw blade 51 is made of a heat transfer member for generating heat by receiving electricity, the first temperature control screw blade (51a), the second temperature control screw blade (51b) ), The third temperature control screw blades 51c are respectively The electric power can be supplied so as to separately generate heat, and the electric power can be cut off from the control unit 70.

Thus, the second temperature control device 50 is installed on the outer periphery of the rotating shaft 21 for stirring located in the reactor 10 to supply heat from the inside of the reactor 10 to the first temperature using the jacket 30 The temperature of the medium can be adjusted to be uniform with the regulator 40,

As shown in FIG. 7, the second temperature adjusting device 50 is disposed in the rotation direction of the stirring shaft 21 by the temperature adjusting screw blade 51 installed in the form of a screw (spiral) on the outer circumference of the stirring shaft 21. As a result, the medium may be moved upward or downward, and the medium may be prevented from moving upward by riding the temperature adjusting screw blade 51 by the rise prevention part 52.

Therefore, the second temperature control device 50 may adjust the temperature so as to be stable and uniform according to the capacity of the medium in the reactor 10 together with the first temperature control device 40 using the jacket 30 to be sewn.

In addition, the rise preventing portion 52 of the second temperature control device 50 made as shown in Figure 6 is located on the surface of the water when the volume of the medium in the reactor 10 is 30Vol%, 50Vol% as shown in FIG. It is desirable to.

The jacket 30 is installed to surround the outer circumferential surface of the reaction tank 10 and indirectly heats the medium in the reaction tank 10 so that the culture of the medium is well performed. The jacket 30 is perpendicular to the reaction tank 10. Heat medium inlet 31 and outlet 32 surrounding the reaction tank 10 to be divided into a plurality of vertical in the vertical direction of the reaction tank 10 to adjust the temperature by heating in different directions (height) is formed in multiple stages Consisting of unit jackets 30a, 30b, and 30c. That is, the jacket 30 is provided with a partition diaphragm on the inner side, and each of the inlet 31 and the outlet 32 for the heat medium and the unit jacket (30a, 30b, 30c) is formed, and is formed in the multi-stage Unit jackets (30a, 30b, 30c) may be made of a plurality of, such as two, three, four, etc., depending on the size of the reaction tank 10, usually consists of two to five.

The jacket 30 may use a plurality of unit jackets (30a, 30b, 30c) to make the heating temperature different depending on the height of the reactor 10 in the vertical direction and at the same time not to cool and heat. Makes it possible.

The first temperature control device 40 is configured to control the temperature of the reaction tank 10 by supplying a heat medium (usually using water) to the jacket 30, the plurality of unit jackets (30a, 30b, 30c) Each is made to supply and cut off the heat medium.

The first temperature control device 40 is a heat exchanger 41 for heating the heat medium discharged through the plurality of outlets 32 formed in the plurality of unit jackets (30a, 30b, 30c), respectively, and the heat medium pipe It consists of a circulating pump 42 to circulate through the movement, the steam supply pipe with a supply control valve (V) is connected to the pipe of each outlet 32 side to supply steam during sterilization treatment Cooling water supply pipes having a supply control valve (V) are connected to the pipes at the respective inlets 31.

In addition, the pipe for moving the heat medium is provided with a plurality of valves (V) for controlling the movement (supply) of the heat medium and a check valve (CV) for preventing backflow, and a plurality of valves (V) installed for the control. It is preferable that a solenoid valve such as a solenoid valve is usually used to be operable by the control unit 70.

The control unit 70 controls a plurality of valves V provided in the pipes on the inlet 31 side and the pipes on the outlet 32 side to supply the heat medium supplied to the plurality of unit jackets 30a, 30b, 30c. The temperature of the heat medium supplied to each of the unit jackets 30a, 30b, and 30c can be controlled differently by using the cooling water supplied through the cooling water supply pipe connected to the inlet 31 side.

The heat insulating material 60 is installed to surround the jacket 30 consisting of a plurality of unit jackets (30a, 30b, 30c) to prevent the heat loss and at the same time protect the reactor 10 and the jacket (30).

The control unit 70 is electrically connected to a plurality of valves (V) consisting of a stirring device 20, a temperature sensor (T), a solenoid valve such as a solenoid valve, and controls the incubator, the stirring device (20) ) Control the operation of the plurality of unit jackets (30a, 30b, 30c) by operating a plurality of valves (V) provided in the piping of the temperature control device 40 by using the information transmitted to the temperature sensor (T). The temperature of the reaction tank 10 is controlled by controlling whether or not the heating medium supplied to the heating medium is supplied.

The operation state of the microorganism incubator having the multi-stage jacket installed as described above will be described with reference to the accompanying drawings, for example, when the amount of the medium is about 50% of the volume Vol of the reactor 10.

The controller 70 supplies steam to the reactor 10 through the air supply pipe 11 and the drain pipe 12 to increase the primary temperature, and supplies steam to the jacket 30 to increase the secondary temperature. The inside of the reactor 10 is sterilized. At this time, it is possible to control the amount of steam used by controlling the steam supplied to the jacket 30 consisting of a plurality of unit jackets (30a, 30b, 30c) according to the amount of medium to be cultured. That is, when the amount of the medium is about 50% of the volume Vol of the reaction tank 10, steam is supplied only to the unit jackets 30a and 30b located at the lower and middle portions of the reaction tank 10, and the unit jacket located at the top. Sterilization may be sufficient for 30c without supplying steam.

When the inside of the reactor 10 is sterilized, and the medium is accommodated in the reactor 10, the control unit 70 is formed of a plurality of unit jackets (30a, 30b, 30c) using the first temperature control device (40). While supplying the heat medium to the jacket 30, the electricity is supplied to the temperature control screw blade 51 of the second temperature control device 50 to generate heat, thereby controlling the temperature of the reaction vessel 10, and stirring device 20. Drive to ensure that the medium is evenly incubated. At this time, the control unit 70 supplies the heat medium only to the unit jackets 30a and 30b positioned in the lower and middle portions by using the first temperature adjusting device 40, and the lower and middle portions of the second temperature adjusting device 50. By applying electricity to the first and second temperature control screw blades (51a, 51b) located in the, the heat for temperature control can be adjusted to a uniform temperature in a short time by supplying from the outside and the inside of the reactor (10) have.

In addition, by blocking the supply of the heat medium, the unit jacket 30c located in the upper portion generates the temperature difference between the inner wall of the reactor located in the medium and air layers and the temperature of the inner wall of the reactor located in the air layer, as described with reference to FIGS. 2 and 3. By preventing the rise, it is possible to suppress the generation of the thermally denatured medium. In particular, when the temperature of the inner wall of the reactor located in the air layer is rapidly increased due to the occurrence of abnormal phenomenon, the temperature control may be induced by supplying a low temperature heat medium to the unit jacket (30c) located in the inner wall of the reactor. In addition, despite the agitation using the stirring device 20, even if a temperature difference occurs between the medium located at the top and the medium located at the bottom, the temperature of the medium is supplied by differently supplying the temperature of the heat medium supplied to the unit jackets 30a, 30b. The temperature difference can be suppressed.

By such a method, it is possible to suppress the generation of the heat-denatured medium generated in the culture of a small amount of medium compared to the volume of the reaction tank 10, and at the same time increase the thermal efficiency and maximize the culture efficiency, thereby To improve productivity and reliability.

8 is a view illustrating a microbial incubator having a multi-stage jacket installed according to another embodiment of the present invention. In order to increase the efficiency of the microbial incubator having the multi-stage jacket illustrated in FIG. The first temperature control device 40 is further provided with a heating device 43 so as to be heated.

That is, as shown in Fig. 8, the heating devices 43a, 43b, 43c are respectively installed in the pipes on the inlet side to heat the heat medium supplied to the unit jackets 30a, 30b, 30c, respectively, and the heating devices 43a, 43b. , 43c is electrically connected to the control unit 70 and controlled by the control unit 70, and the unit jackets 30a, 30b, and 30c may be heated separately from the heat medium supplied to the unit jackets 30a, 30b, and 30c, respectively. The temperature of the heat medium supplied to the can be adjusted differently.

In the case of the microbial incubator in which the multi-stage jacket of FIG. 5 is installed, the temperature of the heat medium supplied to the unit jackets 30a, 30b, and 30c is differently controlled by supplying the cooling water, but in this case, the heat of the heated heat medium is cooled to increase the amount of heat. There is a disadvantage that loss occurs. However, when controlling the temperature of the heat medium supplied to the unit jackets 30a, 30b, 30c by using the heating apparatus 43a, 43b, 43c as shown in FIG. 8, it is possible to suppress the occurrence of heat loss due to the cooling of the heat medium. There are advantages to it.

10: reactor
20: stirring device
30: Jacket unit Jacket: 30a, 30b, 30c
40: first temperature controller
50: second temperature controller
51, 51a, 51b, 51c: screw blade for temperature control
52, 52a, 52b: climb prevention part
60: insulation
70:

Claims (3)

In the incubator provided with a jacket for temperature control on the outside of the reaction tank equipped with a stirring device for stirring the medium,
Reaction tank 10 is composed of a multi-stage unit jacket (30a, 30b, 30c) is provided with a heat medium inlet 31 and an outlet 32 surrounding the reaction tank 10 to be divided into a plurality in the vertical direction of the reaction tank (10) Jacket 30 and made to adjust the temperature differently in the vertical direction of the;
A first temperature controller 40 for controlling temperature by selectively supplying a heat medium to the unit jackets 30a, 30b and 30c, respectively;
The second temperature control is made of a stirring shaft 20 of the stirring device 20 is formed in the form of a screw on the outer periphery, the temperature control screw wings 51 to move the medium in the upper or lower direction according to the rotation direction Including device 50,
The microorganism incubator is installed in the multi-stage jacket, characterized in that the temperature can be controlled to be partitioned in the vertical direction according to the amount of the medium of the reactor (10). The method of claim 1,
The temperature control screw blade 51 is made of a heat-transfer material that is heated by receiving electricity, while the rotating shaft 21 for stirring as a means for preventing the medium rises continuously in the vertical direction riding the temperature control screw blade 51. The microbial incubator is equipped with a multi-stage jacket, characterized in that the rising prevention portion 52 is formed by being installed at a predetermined interval without the temperature control screw wings (51). The method according to claim 1 or 2,
The temperature control device 40 is a multi-stage jacket characterized in that the temperature of the heat medium supplied to the unit jacket (30a, 30b, 30c) divided into a plurality of temperature can be adjusted according to the height of the reaction tank (10) Installed microbial incubator.

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