KR101266528B1 - Moisturizing and/or whitening cosmetic composition comprising polysaccharide extracted from Lentinus edodes mycelium culture product - Google Patents

Abstract

The present invention relates to a cosmetic composition containing shiitake mushroom mycelium culture as an active ingredient. The cosmetic composition containing the shiitake mushroom mycelium culture according to the present invention as an active ingredient is very excellent in moisturizing effect, and also excellent in whitening effect than the arbutin used in the conventional whitening cosmetic composition, and thus may be useful for beauty health.

Description

Moisturizing and / or whitening cosmetic composition comprising polysaccharide extracted from Lentinus edodes mycelium culture product} which contains a polysaccharide extracted from shiitake mycelium culture as an active ingredient

The present invention provides a cosmetic composition for moisturizing and / or whitening containing a polysaccharide extracted from shiitake mycelium culture as an active ingredient.

Skin aging can be divided into two types. One is intrinsic aging, which is an unavoidable aging phenomenon as the years go by. The second is photoaging, which refers to the aging observed in the skin of the face, back of the hand, back of the neck, etc., which has been exposed to sunlight for a long time. Light aging is a skin aging phenomenon that can be prevented by avoiding exposure to ultraviolet light. Endogenous aging is mainly observed in skin that is not exposed to sunlight.

Clinical features are relatively mild and include fine lines, dry skin, and reduced elasticity. However, the clinical characteristics of light aging are severe compared to endogenous aging and are observed early. Compared with endogenous aging, thick and deep wrinkles occur, and fine wrinkles also occur a lot.

Irregular pigmentation occurs in the skin exposed to sunlight and pigment diseases such as solar lentigo increase. The skin is very rough, dry and less elastic, which in the worst case sags the skin.

Wrinkles are caused by the distribution of muscles and the movement of muscles on the face. If you always look with a mixture of irritation on the face, the thick wrinkles of the inner neck (gawa) occurs on the forehead, and the face that is always smiling tends to have a lot of fine lines around the eyes.

Genetic predisposition also affects wrinkles on the face. There are many differences in the appearance of wrinkles by race. Wrinkles in western people and wrinkles in Koreans tend to show a lot of difference. Koreans tend to have thicker wrinkles than Westerners, with thicker wrinkles around the forehead, eyes, and mouth. Whites, on the other hand, tend to have fine lines on their foreheads and cheeks rather than thick wrinkles. In Korea, there are people who look older because they have more wrinkles than their age. There are also people who do not have many wrinkles even when they are old people. It is not known if there are any genes associated with wrinkles, but it is clearly genetically affected.

Furthermore, ultraviolet rays contained in sunlight cause wrinkles on the skin. Skin that has been exposed to ultraviolet light for a long time, such as the face, has been reported to produce thicker and more wrinkles.

In addition, in women, wrinkles occur more frequently after menopause and incidence of wrinkles is reduced in women taking estrogen after menopause than in women who do not take it. As a result, it has been reported that the reduction of estrogen after menopause worsens wrinkles of skin, and other hormones besides estrogen have also been reported to play an important role (Health Industrial Technology Trends, 29 ~ 30, 2003).

Recently, to treat skin aging such as wrinkles, retinoic acid (vitamin A), alpha-hydroxy acids (alpha-hydroxy acids), antioxidants, and colorants are applied to the skin topically. have. Surgical methods include laser dermabrasion, trichloroacetic acid, chemical dermabrasion using phenol, and botium toxin injection. Functional wrinkle improvement raw materials specified by the Cosmetics Act in Korea include retinol, retinyl palmitate, adenosine and polyethoxysyltedinamide.

However, the above-mentioned materials are synthetic compounds, and in order to satisfy the requirements of modern people seeking well-being, the use of natural products has been prominent in the cosmetic field in recent years, and it has excellent moisturizing ability, aging improvement function, and antioxidant effect, and especially skin aging improvement. Many studies have been made on cosmetic compositions using natural products that are reported to be excellent in terms of effects.

Shiitake mushroom (Lentinus edodes) is a fungus that grows from dead or dead branches of hardwoods such as oaks (auburn tree, oak tree, oyster oak, prunus oak), seobo, chestnut tree, etc. As a white or white woody mushroom that grows or grows in the stomach, it has been treated as one of the three major edible mushrooms in Korea.

Shiitake mushrooms were ranked first among the top 10 foods that raise cholesterol (HDL) and lower bad cholesterol (LDL), and were published by the American Heart Association in January 2007. Shiitake mushrooms contain anti-cancer and anti-tumor polysaccharides. Contains lentinan (Lentinan) has been developed as a drug that currently suppresses the increase of immunity and proliferation of cancer cells.

In addition, shiitake mushroom has an ingredient called ritadenine (Eritadenine), which removes cholesterol from the blood and lowers blood pressure, thereby preventing hypertension, and the spores of shiitake are infected with influenza virus (flu) as an interferon-inducing substance. It has been reported to be effective in preventing and soluble lignin glycoprotein complexes have been reported to be effective against HIV (HIV).

In addition, it is known to be effective in the treatment of tonic, diuretic, hypertension, nephritis, nervous breakdown, insomnia, asthma, gastric ulcer, etc. In Korea Patent Publication No. 195-0016763 Shiitake mushroom mycelium extract prepared using shiitake fungus An HIV activity inhibitor containing an active ingredient has been disclosed, and Korean Patent Laid-Open Publication No. 2001-0089497 discloses a therapeutic agent for treating tumors, bacterial infections or viral infections using shiitake mushroom mycelium extract having an effect of enhancing γδ T cell activity. It is starting.

Accordingly, the present inventors while studying to develop a cosmetic composition using a natural product having no side effects and excellent moisturizing power and / or whitening effect, confirming that the polysaccharide extracted from shiitake mushroom mycelium culture is effective for skin improvement, and moisturizing And / or the present invention was completed by revealing that it can be usefully used as a cosmetic composition for whitening.

An object of the present invention is to provide a moisturizing cosmetic composition containing a polysaccharide extracted from shiitake mushroom mycelium culture as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for whitening containing a polysaccharide extracted from shiitake mycelium culture as an active ingredient.

In order to achieve the above object, the present invention provides a moisturizing cosmetic composition containing a polysaccharide extracted from shiitake mycelium culture as an active ingredient.

The present invention also provides a cosmetic composition for whitening containing the polysaccharide extracted from shiitake mycelium culture as an active ingredient.

The cosmetic composition containing the polysaccharide extracted from shiitake mushroom mycelium culture according to the present invention as an active ingredient is very excellent in moisturizing effect, and also excellent in whitening effect than arbutin used in conventional whitening cosmetics, which is useful for beauty health. Can be.

Hereinafter, the present invention will be described in detail.

The present invention provides a moisturizing cosmetic composition containing a polysaccharide extracted from shiitake mycelium culture as an active ingredient.

In addition, the polysaccharide extracted from the shiitake mushroom mycelium culture according to the present invention, after culturing shiitake mushroom strains, the culture filtrate and mycelium are separated, the culture filtrate is concentrated, and then the polysaccharide is extracted using alcohol, Pure polysaccharides can be obtained by dissolving polysaccharides in water to remove insolubles.

Specifically, inoculated with shiitake mushroom seedlings in agar medium, incubated in a flask containing a liquid medium by removing the cultured strain pieces, and incubated, the stirring speed and dissolved oxygen amount in the same medium to the growth rate of the mycelia. Increase and incubate permanter for 48-96 hours. In addition, after completion of the culture, the polysaccharide is recovered by using an alcohol in the filtrate, the polysaccharide is dissolved in water to remove the insolubles to obtain the shiitake mushroom polysaccharide from which impurities are removed.

Furthermore, the polysaccharide extracted from the shiitake mycelium culture according to the present invention may be added in an amount of 15 to 30 parts by weight, preferably 20 to 25 parts by weight, but is not limited thereto.

As a result of evaluating the cream prepared from the polysaccharide extracted from shiitake mycelium culture according to the present invention in 50 adult females, 68% of the testers responded that the skin condition was very good, and 20% of the testers When the skin condition was improved, it was confirmed that the skin condition was improved and the moisturizing effect was excellent (see Table 2).

The present invention also provides a cosmetic composition for whitening containing the polysaccharide extracted from shiitake mycelium culture as an active ingredient.

Further, the polysaccharide extracted from the shiitake mycelium culture may be added in an amount of 30 to 50 parts by weight, preferably 40 to 50 parts by weight, but is not limited thereto.

Polysaccharide extracted from shiitake mushroom mycelium culture according to the present invention was shown to have a superior effect of inhibiting tyrosinase than the lettuce extract known to have excellent antioxidant activity among the conventional cosmetic compositions for whitening and natural products (see Table 4). As a result of evaluating 50 adult female creams prepared using shiitake mycelium cultures, it was confirmed that the whitening effect was excellent (see Table 5).

The cosmetic composition may include, but is not limited to, lotions, ointments, gels, creams, patches or sprays. In preparing the moisturizing and / or whitening cosmetic composition containing the polysaccharide extracted from the shiitake mushroom mycelium culture of the present invention, the polysaccharide extracted from the shiitake mushroom mycelium culture of the present invention is included in the composition for external application for skin. It may be added in 3 to 30 parts by weight, preferably 5 or 20 parts by weight.

In the cosmetic composition of the present invention, in addition to the polysaccharide extracted from the shiitake mycelium culture of the present invention, fatty substances, organic solvents, solubilizers, thickening agents and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents (foaming agents) ), Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipids It may contain adjuvants commonly used in the field of dermatology, such as vesicles or any other ingredients conventionally used in cosmetic compositions.

The ingredients may also be introduced in amounts generally used in the field of dermatology.

Hereinafter, the present invention will be described by production examples and examples.

However, the following Preparation Examples and Examples are merely to illustrate the present invention, and the content of the present invention is not limited to the following Preparation Examples and Examples.

< Manufacturing example  1> Culture of Shiitake Strains

Shiitake mushroom strain (HKSY00025) was used to separate the tissue from the fruiting body purchased in Daejeon Doma market.

In order to liquid culture the strains, the seed was inoculated in PDA (MERK Co., Ltd.) agar medium and incubated for 10-20 days at 20-30 ℃.

When the diameter of the strain was incubated to about 50-60 mm, the tissue was separated from the PDA plate by using Corkborough to remove 10 pieces of 5 mm in diameter, 4 g of glucose, 4 g of yeast extract, and 10 g / l of malt extract). Inoculated into a flask containing the YMG liquid medium consisting of, and incubated for 5-10 days at 25-30 ℃.

The seed culture incubated in the liquid medium using the same YMG medium as the liquid medium, and incubation at 25 ℃ for 3 to 10 days at a stirring speed of 150 rpm, the initial amount of oxygen 0.5 vvm, the stirring speed and dissolved oxygen amount Was increased according to the growth rate of the mycelium and cultured for permanter for 48 to 96 hours.

After completion of the culture, since the viscosity of the culture was difficult to separate the cells, the filter paper was collected from the filter paper with a large mesh size using a filter paper with a small mesh size (10 to 35 mesh), and then the filtrate was collected. At this time, whatmann-113 was used as a filter paper.

The collected filtrate was recovered from polysaccharides using ethanol, and then the polysaccharide was dissolved in water to remove insolubles, thereby obtaining shiitake mushroom polysaccharides from which impurities were removed.

< Example  1> Moisturizing skin using shiitake mycelium culture Cosmetics  Preparation of the composition

Shiitake mushroom culture solution prepared in Preparation Example 1 was mixed with the cosmetic composition raw materials of Table 1 to prepare a cosmetic composition exhibiting skin improvement and moisturizing effect.

Raw material name Weight (mg) Shiitake mycelium culture 20 Wax 4.5 Polysorbate-60 2.0 Sorbitan sesquinolate 0.5 Liquid paraffin 7.0 Squalene 10.0 Sorbitan stearate 1.5 PEG-400 Stearate 1.5 Lipophilic monostearic acid glycerin 0.5 Stearic acid 1.5 BG (butylene glycol) 3.0 PG (propylene glycol) 3.0 TEA (triethanolamine) 0.2 Preservative a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

< Comparative example  1> Skin improvement and moisturizing effect measurement

Except not using shiitake mycelium culture in Example 1 to prepare a cream containing shiitake mushroom mycelium culture using the same amount of raw materials, 20 ~ 50 having dry, normal, complex skin After washing the face in half for women, the skin of Example 1 was coated on one side and the cream of Comparative Example 1 was applied on the other side for 20 days. The results are shown in Table 2 below.

division Skin condition improvement Survey item Number of people %
Example 1 very good 34 68 good 10 20 is average 4 8 so so 2 4
Comparative Example 1 very good 0 0 good 10 20 is average 18 36 so so 22 44

As shown in Table 2, when the cream without the shiitake mycelium culture of Comparative Example 1 was applied, 20% of the test subjects felt that the skin condition was improved, while the shiitake mycelium culture according to the present invention was applied. When applying the cream prepared using, 68% of the testers responded that the skin condition was very good, and 20% of the testers responded that the skin condition improved.

Therefore, shiitake mushroom mycelium culture of the present invention can be usefully used as a cosmetic composition because it is confirmed that the skin condition improvement and moisturizing effect is excellent.

< Experimental Example  1> Tyrosinase  Measurement of inhibitory effect

In order to measure the inhibitory effect of tyrosinase activity, an enzyme related to the whitening activity of shiitake mushroom mycelium culture prepared in Preparation Example 1, it was measured using mushroom tyrosinase. As a control, Arbutin, the main ingredient of the extract of Morus alba and the conventional whitening cosmetic composition, was used.

15 µl of shiitake mushroom mycelium culture, lettuce extract and arbutin were put into 96 well plates, respectively, and 150 µl of 50 mM phosphate buffer solution (pH 6.5) and 25 µl of 1.5 mM L-tyrosine solution were added. 10 μl of mushroom tyrosinase (Sigma-Aldrich Co., Ltd.) was added to the mixture and reacted at 37 ° C. for 20 minutes, followed by 490 using a microplate reader (VERSA max / Molecular Devices, USA). Absorbance was measured at nm.

The inhibition rate for tyrosinase is calculated by Equation 1 below, and the IC ₅₀ value represents a concentration that inhibits tyrosinase enzyme activity by 50%, and the results are shown in Table 3 below.

In Equation 1,

A: Absorbance before reaction of the well into which the sample was placed

B: Absorbance after the reaction of the well into which the sample was placed

C: Absorbance before reaction of the well without sample

D: Absorbance after reaction of the well without sample

sample Inhibitory effect of mushroom tyrosinase (IC ₅₀ ) Arbutin 0.35 mg / ml Eucalyptus extract 1.0 mg / ml Production Example 1
Shiitake mushroom strain culture 0.18 mg / ml

As shown in Table 3, the shiitake mushroom mycelium culture according to the present invention has an IC ₅₀ value of 0.18 mg / ml than that of arbutin (IC ₅₀ = 0.35 mg / ml), which is much used in conventional cosmetic compositions for whitening. The effect was shown to be two times better, and the tyrosinase inhibitory effect was found to be about 6 times better at IC ₅₀ value of 0.18 mg / ml than the extract of natural extract, IC ₅₀ = 1.0 mg / ml.

Therefore, the shiitake mushroom mycelium culture according to the present invention has a superior effect of inhibiting tyrosinase in the conventional whitening cosmetic composition and natural products, which is known to have excellent antioxidant activity, and thus can be usefully used in the cosmetic composition for whitening.

< Example  2> Skin whitening using shiitake mycelium culture Cosmetics  Preparation of the composition

Shiitake mushroom culture solution prepared in Preparation Example 1 was mixed with the cosmetic composition raw materials of Table 4 to prepare a cosmetic composition exhibiting skin improvement and moisturizing effect.

Raw material name Weight (mg) Shiitake mycelium culture 40.0 Stearyl alcohol 7.0 Stearic acid 2.0 Stearic Acid Cholesterol 2.5 Squalene 4.5 2-octyldodecyl alcohol 6.0 Polyoxyethylene (25 mol)
Octyldodecyl alcohol 2.5 Glyceryl
Monostearic acid ester 2.0 PG (Propylene Recall) 4.0 Purified water Proper amount

< Comparative example  2> skin whitening effect measurement

Except not using shiitake mycelium culture in Example 2 to prepare a cream containing shiitake mushroom mycelium culture using the same amount of raw material, 20 ~ 50 having dry, normal, complex skin After washing the face in half for women, the skin of Example 2 was applied to one side and the cream of Comparative Example 2 was applied to the other side for 20 days once a day. The results are shown in Table 5 below.

division Skin condition improvement Survey item Number of people %
Example 2 very good 36 72 good 8 16 is average 5 10 so so One 5
Comparative Example 2 very good 0 0 good 15 30 is average 17 34 so so 18 36

As shown in Table 5, when the cream without the shiitake mycelium culture of Comparative Example 2 was applied, 30% of the testers responded that the whitening effect was good, whereas the shiitake mycelium culture according to the present invention was used. When applying the cream prepared using, 72% of the testers answered that the whitening effect was very good, and 16% of the testers answered that the whitening effect was good.

Therefore, the shiitake mushroom mycelium culture of the present invention can be usefully used as a cosmetic composition because it is confirmed that the skin condition improvement and moisturizing effect as well as the whitening effect is excellent.

< Formulation example  1> Cosmetics  Preparation of the composition

The present inventors prepared a cosmetic composition containing the polysaccharide extracted from the shiitake mushroom mycelium culture as an active ingredient as follows.

1. Preparation of Cream

Cetostearyl alcohol 2.8 parts by weight

2.6 parts by weight of beeswax

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

Shiitake mushroom mycelium culture polysaccharide 4.6 parts by weight

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

p-H2O 46.05 parts by weight

2. Preparation of Lotion

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

Shiitake mushroom mycelium culture polysaccharide 3.5 parts by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

p-H20 48.3 parts by weight

Claims (8)

delete delete delete delete A whitening cosmetic composition containing the polysaccharide extracted from shiitake mycelium culture as an active ingredient.
The method according to claim 5, wherein the polysaccharide extracted from the shiitake mycelium culture, after culturing shiitake mushroom strains, the culture filtrate and mycelium are separated, the culture filtrate is concentrated, and then the polysaccharide is extracted using alcohol, A whitening cosmetic composition, characterized in that the polysaccharide is dissolved in water to obtain an insoluble matter.
The cosmetic composition for whitening according to claim 5, wherein the shiitake mushroom mycelium culture is added in an amount of 30 to 60 parts by weight.
The whitening cosmetic composition according to claim 7, wherein the shiitake mycelium culture is added in an amount of 40 to 50 parts by weight.