KR101207317B1 - A Composition For Promote Eyebrow And Eyelash And Manufacturing Method Thereof And The Cosmetic Composition Comprising Thereof - Google Patents

Abstract

The present invention relates to a composition for promoting eyebrow and eyelash growth, including the extract of Jinju green medicinal herb extract, tissue cultured wild ginseng culture root extract, and tissue cultured Geumnyeonryeon extract, a preparation method thereof, and a cosmetic comprising the same.
The composition for promoting eyebrow eyebrow and eyelash growth, the preparation method thereof, and the cosmetic comprising the same have an effect of continuously increasing the absorption effect of an immunoactive substance on the eyebrows and eyelashes to maintain hair follicle activity. In addition, the sebum produced by using the antioxidant activity of the composition to remove the accumulation of lipid peroxide continuously to promote the growth of eyebrows and eyelashes, and has the effect of completing a dark and clear eye, and eye irritation by using a natural extract There is little effect.

Description

Composition for promoting eyebrow and eyelash growth, a method for manufacturing the same and a cosmetic comprising the same {A Composition For Promote Eyebrow And Eyelash And Manufacturing Method Thereof And The Cosmetic Composition Comprising Thereof}

The present invention relates to a composition for promoting eyebrow and eyelash growth, a method for manufacturing the same and a cosmetic comprising the same, and more specifically, an eyebrow including an extract of Kumju green milk powder, tissue cultured wild ginseng culture root extract and tissue cultured gold sesame extract And it relates to a composition for promoting eyelash growth, a manufacturing method and a cosmetic comprising the same.

With the increasing interest in beauty in the modern society, the desire for the function of cosmetics is increasing, and high functional products are continuously appearing accordingly.

Makeup can change the image of the face. In particular, eye makeup is the most convenient and effective part of the face that can change the impression. In eye makeup, the thicker and richer the eyebrows and eyelashes make the face look clearer. Therefore, various functional products for eyebrows and eyelashes are being developed.

Eyebrows and eyelashes differ markedly from other parts of hair, such as the hair of the scalp, in terms of cycles of growth and rest. In the case of hair, the growth phase lasts about 3 to 4 years, the resting period lasts for 3 to 4 months, the growth phase accounts for 85 to 93% of the entire cycle, and the resting phase accounts for 7 to 15% of the entire cycle. The growth rate is about 0.4 mm / day. On the other hand, in the case of eyebrows and eyelashes, the growth phase is 6-10 weeks, the resting period is maintained for 4-9 months, the growth phase accounts for 10-20% of the entire cycle, and the resting period is 80-90 It accounts for%, the growth rate of eyebrows and eyelashes is about 80 ~ 280 ㎛ / day, a significant difference from the hair growth rate.

As such, the eyebrows and eyelashes have different growth phases, resting periods and growth rates than the hairs, and thus, when a composition such as minoxidil and propecia has been used for the growth of eyebrows and eyelashes, which has been used for the conventional hair promotion, Has disadvantages that are not outstanding.

In order to solve such a problem, Korean Patent Publication No. 2010-0026335 discloses an eyebrow or eyelash growth promoting composition containing 4-pyrrolidine 2,6-diaminopyrimidine 1-oxide, but it is natural It is not a composition using an extract and may cause eye irritation.

Therefore, in recent years, there has been a method of preparing an extract containing the natural plant itself in the cosmetic composition, but the process of sterilizing the plant itself, the size of the plant added to the composition, the cultivation environment, etc. are different, so that the uniformity of the plant extract quality There is a downside to falling.

The present invention is to solve the above problems, an object of the present invention is to promote the growth of the eyebrows and eyelashes, harmless to the human body, the composition for promoting eyebrow and eyelash growth to minimize the irritation to sensitive eye parts, its It provides a manufacturing method and a cosmetic comprising the same.

In order to solve the object as described above, the present invention is a composition for promoting eyebrow and eyelash growth, eyebrow and eyelash growth promoting comprising a extract of Jinju green milk crude extract, tissue cultured mountain ginseng culture root extract and tissue cultured Geumnyeon Ryun extract To provide a composition.

In addition, the present invention provides a composition for promoting eyebrow and eyelash growth that may further include adding a plant complex extract.

In addition,

a) mixing a solvent with each of the Jinju lychee milk herbal mixture, tissue cultured wild ginseng culture root, and tissue cultured geumsunyeon;

b) aging and filtering the respective mixtures obtained in step a), and then preparing each extract to obtain the filtrate; And

c) provides a method for preparing a composition for promoting eyebrow and eyelash growth, comprising a step of preparing a composition comprising the extract of Jinju lychee milk extract obtained in step b), the cultured wild ginseng cultured root extract, and the tissue cultured gold sesame extract.

The composition for promoting eyebrow and eyelash growth of the present invention, a preparation method thereof, and a cosmetic including the same have an effect of continuously increasing the absorption effect of an immunoactive substance on the eyebrows and eyelashes to maintain hair follicle activity. In addition, the sebum produced by using the antioxidant activity of the composition to remove the accumulation of lipid peroxide continuously to promote the growth of eyebrows and eyelashes, and has the effect of completing a dark and clear eye, and eye irritation by using a natural extract There is little effect.

The present invention relates to a composition for promoting eyebrow and eyelash growth, comprising a extract of Geumju-kungwoon crude herb extract, tissue cultured mountain ginseng culture root extract, and tissue-cultured Geumnyeon-ryeon extract. To 80% by weight, Tissue cultured wild ginseng culture root extract 9 to 80% by weight and Tissue cultured Geumnyeon Ryun extract Provides a composition comprising .

Here, the composition for promoting eyebrow and eyelash growth may further include a plant complex extract, and preferably further comprises 0.1 to 30% by weight of the plant complex extract based on the total weight of the composition.

As defined in the present invention, "Jinzhou green walnut milk herbal extract" means the extract of the medicinal herb extract of Jinzhou green walnut milk, which is listed as having the effect of making hair on the outer surface of Dongbogam, mantyeong, copper leaf, aroma, nasturtium, windproof, It refers to five or more mixtures selected from the group consisting of Bokbunja, Saenghwanghwang, Yeongneunghyang, Manganese, Clove, Kwonbaek, Chuckhwa and Gazapi.

In this case, the extract of Jinzhou green walnut milk herbal medicine is 1 to 20% by weight, 1 to 20% by weight, 1 to 20% by weight, aroma 1 to 20% by weight, windproof 1 to 20% by weight relative to the total weight of the extract 20% by weight, 1-20% by weight bokbunja, 1-20% by weight of raw yellow sulfur, 1-20% by weight of Youngneung, 1-20% by weight, clove 1-20%, 1-40% by weight, 1-40% by weight 20 wt% and 1-20 wt% of Gazapi are preferably mixed and included, but are not limited thereto.

The extract of Jinju green biloba milk is preferably 9 to 80% by weight relative to the total weight of the composition for promoting eyebrow and eyelash growth, when included in the content to alleviate the skin irritation, grow by supplying nutrition to the hair root, hair follicles to the effect of acceleration.

The tissue cultured wild ginseng culture root extract can be used if it is prepared by a method commonly used in the art, and drying the tissue cultured wild ginseng culture root; Aging by adding a solvent to the dried ginseng culture root; And filtering the matured extract. Preferably, the method may further include a solvent removing step after the filtering step.

At this time, as a method of manufacturing cultured wild ginseng root,

After sterilization of wild ginseng, cut into sections of 2∼3㎜ and add 2, 4-D (2,4-dichlorophenoxy aceticacid; IBA), Pichloram, and NAA (naphthalemeacetic acid) in the amount of 1.0∼10.0mg / L Inoculating one MS (Murashige-Skoog) medium and inducing callus;

The induced callus was propagated in MS medium to which 2,4-D (2,4-dichlorophenoxy aceticacid) was added 0.1 to 5.0 mg / L, and then subcultured at intervals of 2 to 4 weeks, and either IBA or NAA was 1.0. Transferring to MS medium added in an amount of ˜5.0 mg / L to form a root muscle;

Propagating the formed root muscle in MS medium;

The proliferated intestinal root was inoculated into an air flotation balloon-type bioreactor, and BSAA (benzo [b] selenienyl acetic acid) 1.0-10.0 mg / L, IBA 1.0-10.0 mg / Culturing by adding any one of L and NAA 1.0-10.0 mg / L;

It is preferable to use a method for mass propagation of tissue cultured wild ginseng culture root, including the step of scaling up the cultured involuntary root with a larger capacity bioreactor.

The ginseng root extract of the tissue culture is preferably containing 9 to 80% by weight relative to the total weight of the composition for promoting eyebrow and eyelash growth, when included in the content to reduce skin irritation and promote cell growth while immune active ingredients It has the effect of enhancing hair follicle activity .

Geumsunyeon is also a rare egg plant in the Himalayan region of Indochina, relaxation, Vietnam, and Japan. Expensive gold sesame, which is used as a Chinese herb, has various pharmacological effects such as anticancer, high blood pressure, diabetes, etc., and as the demand increases, the presence of natural products is severely reduced, which is important in Taiwan's herbal market.

Geumsunyeon extract of the tissue culture can be used if it is prepared by a method commonly used in the art, as a culture culture method of Geumsunyeon, inoculated in the Hyphenex medium containing coconut juice and activated carbon, 30 ~ 70 It is preferable to use a tissue cultured gold herb obtained by mass-promoting the gold herb via a method comprising culturing the gold sesame under the condition of m ⁻² s ⁻¹ light intensity sustained density and continuous immersion culture system.

Geumsunyeon extract of the tissue culture is preferably containing 9 to 80% by weight relative to the total weight of the composition for promoting eyebrow and eyelash growth, and when included in the content has the effect of forming a protective film of hair follicles by maintaining a continuous moisturizing effect There is .

In addition, the eyebrow and eyelash growth promoting composition of the present invention may further comprise a plant complex extract, the plant complex extract is preferably one or two mixtures selected from the group consisting of iris extract, green tea extract, iris When the extract and the green tea extract are mixed, the preferred weight ratio of the two is 1: 9-9: 1.

Plant complex extract including iris extract, green tea extract is to maintain the activity of hair follicles by increasing the absorption effect of the immune active material to the eyebrows and eyelashes when included 0.1 to 30% by weight relative to the total weight of the eyebrow and eyelash growth promoting composition Simultaneous removal of sebum produced at the same time as lipid peroxide by using the antioxidant activity of the complex extract promotes eyebrow and eyelash growth.

The extract of Jinzhou green walnut milk herbal extract, tissue cultured wild ginseng culture root extract, tissue cultured Geumnyeon Ryun extract and plant complex extract is water containing purified water; Lower alcohols having 1 to 4 carbon atoms; Hexane; 1,3-butylene glycol; Propylene glycol; Pentylene glycol; glycerin; Acetic acid; ether; And it may be a component extracted using one or two or more mixtures selected from the group consisting of chloroform as a solvent.

When making a cosmetic comprising the composition for promoting eyebrow and eyelash growth, it is preferable to include 0.1 to 50% by weight of the composition for promoting eyelashes and eyelashes and 50 to 99.9% by weight of the cosmetic excipient with respect to the total weight of the cosmetic composition.

Here, when the composition for promoting eyebrow and eyelash growth is less than 0.1% by weight, the effect of promoting the growth of the eyebrows and eyelashes is insignificant, and when it contains more than 50% by weight, the inherent aroma according to the inherent flavor of the herbal medicine is dark There is difficulty in applying the product.

The cosmetic excipient may be used as long as it is a conventional ingredient for manufacturing cosmetics.

The cosmetics may be formulated into one or more selected from the group consisting of liquids, sprays, gels, pastes and emulsions.

In addition, the cosmetics, mascara, eyeliner, eye shadow, eyebrow, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nutrition lotion, nutrition cream, essence, nutrition essence, pack selected from the group consisting of 1 It may be formulated with more than one species, and preferably as an eye makeup cosmetic that can be used for eyebrows and eyelashes, it may be formulated with one or more selected from the group consisting of mascara, eyeliner eyeshadow, and eyebrow.

In addition, a) mixing a solvent to each of the kumju green milk crude herbal mixture, tissue cultured wild ginseng culture root and tissue cultured geumsunyeon;

b) aging and filtering the respective mixtures obtained in step a), and then preparing each extract to obtain the filtrate; And

c) provides a method for preparing a composition for promoting eyebrow and eyelash growth, comprising a step of preparing a composition comprising the extract of Jinju lychee milk extract obtained in step b), the cultured wild ginseng cultured root extract, and the tissue cultured gold sesame extract.

In step a), the solvent may be mixed with one kind of the herbal mixture of Jinju green walnut milk, the cultured wild ginseng culture root, and the cultured geumsunyeon, or the solvent may be mixed with two or more mixtures.

Here, after the b) extract preparation step, it may further comprise the step of removing the extraction solvent at 50 to 60 ℃.

At this time, the total weight of the composition may include 9 to 80% by weight of the extract of green wine medicinal herb extract, 9 to 80% by weight of the ginseng culture root extract cultured tissue and 9 to 80% by weight of the extract of tissue culture gold.

In addition, the step C) may further comprise 0.1 to 30% by weight of the plant complex extract based on the total weight of the composition.

The plant complex extract is preferably a mixture of one or two selected from the group consisting of iris extract, green tea extract.

The solvent is water, including purified water; Lower alcohols having 1 to 4 carbon atoms; Hexane; 1,3-butylene glycol; Propylene glycol; Pentylene glycol; glycerin; Acetic acid; ether; And one or two or more mixtures selected from the group consisting of chloroform, but is not limited thereto.

Subsequently, 0.1 to 50% by weight of the composition for promoting eyebrow and eyelash growth and the cosmetic excipient 50 to 99.9% by weight based on the total weight of the cosmetic may be mixed to prepare a cosmetic.

Hereinafter, the present invention will be described in detail with Examples and Experimental Examples.

However, Examples and Experimental Examples are only for illustrating the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

Preparation Example 1 Preparation of Geumju Lungwoon Extract

In order to prepare the extract of Jinju green biloba milk herb, the components of Mansi-shaped, guritdae, aroma, nasturtium, windproof, bokbunja, raw jihwang, Yeongreung incense, forget-me-not, cloves and white wine after drying and cutting, , Nasturtium, windproof, bokbunja, raw ground yellow, Yeongneung, manganese, clove and baekbaek were added in the same amount to 3 kg of 70% ethanol aqueous solution to the same amount, and then stirred and extracted for 3 days and then filter paper (Whatman No .5, whatman international Ltd, UK). The filtrate was concentrated under reduced pressure to obtain a Jinju green bran oil extract powder.

Preparation Example 2 Preparation of Tissue Cultured Wild Ginseng Cultured Root Extract

Tissue cultured wild ginseng root of the present invention is tissue cultured by the tissue culture method described in Korean Patent No. 0353636 and mass propagated, 30 kg of 70% ethanol and 1,3 in 1 kg of dried tissue cultured wild ginseng culture root. -3 kg of butylene glycol or 3 kg of propylene glycol were mixed. Then, after aging sufficiently, the resultant was filtered with a filter paper (Whatman No. 5, whatman international Ltd, UK) to obtain an extract, and an extract obtained by removing ethanol at 50-60 ° C. was obtained.

Preparation Example 3 Preparation of Tissue Culture Geumnyeon Ryun Extract

Geumsunyeon cultured in the tissue of the present invention is characterized by being obtained by the tissue culture method described in Korean Patent Application No. 10-2007-0089698. 30 kg of 70% ethanol and 3 kg of 1,3-butylene glycol or 3 kg of propylene glycol were mixed with 1 kg of dried tissue cultured gold wire. Then, after aging sufficiently, the resultant was filtered with a filter paper (Whatman No. 5, whatman international Ltd, UK) to obtain an extract, and an extract obtained by removing ethanol at 50-60 ° C. was obtained.

Preparation Example 4 Preparation of Plant Complex Extract Containing Iris and Green Tea

The plant complex mixture of irises and green tea was dried and chopped, and 3 liters of irises and green tea were added to 3 L of 70% ethanol aqueous solution at a ratio of 1: 1 to 1 kg, followed by stirring extraction for 3 days, followed by filter paper (Whatman No. 5, whatman international Ltd, UK). The filtrate was concentrated under reduced pressure to obtain a composite extract.

Example 1 Preparation of a Composition Having Eyebrow and Eyelash Growth Promoting Effects

45.0% by weight of the extract of Jinju lychee milk of Preparation Example 1, 30.0% by weight of the tissue culture wild ginseng culture root extract of Preparation Example 2, 25.0% by weight of the tissue culture Geumnyeon extract of Preparation Example 3 to prepare a composition for promoting eyebrow and eyelash growth It is shown in [Table 1].

(Unit: wt%) Composition water content Jinju Green Milk Milk Herb Extract 45.0 Wild Ginseng Culture Root Extract 30.0 Geumnyeonryeon Extract 25.0 Sum 100.0

Experimental Example 1: Eyelash growth promoting effect test

In order to examine the effects of the eyelash and eyelash growth promoting composition obtained in Example 1, the adult women were tested once a day before bedtime, after washing the face to test the growth effect of the eyelashes.

By treating the concentration of the composition prepared in Example 1 at a concentration of 0.5, 1.0, 2.5, 5% as shown in Table 2, the effect of the eyelash and eyelash growth promoting composition on the growth effect of the eyelashes. In order to observe the growth effect of eyelashes over time, eyelash lengths were measured using an image analyzer (Image analyzer, DP701 controller, Olympus) on day 0 and day 42 and are shown in Table 2.

sample Eyelash Length (mm) Control 6.9 ± 1.1 Compositions Prepared By Example 1 0.5% 7.2 ± 1.5 1.0% 7.5 ± 1.1 2.5% 8.2 ± 1.7 5.0% 8.8 ± 1.4

As a result, about 1.9 mm growth was promoted over time by the composition prepared in Example 1, showing a concentration-dependent growth rate of the extract. Therefore, it was confirmed that the growth effect of eyebrow and eyelash growth by the extract of Jinzhou green walnut milk herbal extract, tissue cultured wild ginseng culture root extract, tissue cultured gold sesame extract contained in the Geumju green walnut milk prescription contained in the consent of the present invention.

Example 2 Preparation of a Composition Having Eyebrow and Eyelash Growth Promoting Effects

30.0% by weight of the extract of Jinju green milk powder of Preparation Example 1, 30.0% by weight of the tissue culture wild ginseng culture root extract of Preparation Example 2, 25.0 wt% of the tissue culture Geumnyeon lotus extract of Preparation Example 3, and the iris extract of Preparation Example 4, green tea extract 15.0 wt% of the plant complex extract was mixed to prepare a composition for promoting eyebrow and eyelash growth, and is shown in [Table 3].

(Unit: wt%) Composition water content Jinju Green Milk Milk Herb Extract 30.0 Wild Ginseng Culture Root Extract 30.0 Geumnyeonryeon Extract 25.0 Plant complex extract 15.0 Sum 100.0

Experimental Example 2: Eyelash growth promoting effect test

In order to examine the effect of the eyelash and eyelash growth promoting composition obtained in Example 2, the adult women were tested once a day before bedtime, after washing the face to test the growth effect of the eyelashes.

By treating the concentration of the composition prepared in Example 2 to a concentration of 0.5, 1.0, 2.5, 5% as shown in Table 2 below to determine the effect of the eyelash and eyelash growth promoting composition on the growth effect of the eyelashes. In order to observe the growth effect of eyelashes over time, eyelash lengths were measured using an image analyzer (Image analyzer, DP701 controller, Olympus) on day 0 and day 42 and are shown in Table 4.

sample Eyelash Length (mm) Control 7.0 ± 0.9 Compositions Prepared By Example 1 0.5% 7.3 ± 1.2 1.0% 7.4 ± 1.7 2.5% 8.1 ± 1.4 5.0% 9.3 ± 1.5

As a result, about 2.5 mm growth was promoted over time by the composition prepared in Example 2, showing a concentration-dependent growth rate of the extract. Therefore, the composition for promoting eyebrow and eyelash growth by the plant complex extract including Jinzhou green buckwheat herbal medicine extract, tissue cultured wild ginseng culture root extract, tissue cultured gold sesame extract and iris extract, green tea extract is the plant complex extract By further comprising, it was confirmed that the eyebrow and eyelash growth promoting effect can be further enhanced.

Hereinafter, a formulation example of a composition including the extracts of the present invention will be described, but the present invention is not intended to be limited thereto but only to be described in detail.

Formulation Example 1 Preparation of Eyeliner

To the composition ratio shown in Table 5, to prepare an eyeliner comprising a composition for promoting eyebrow and eyelash growth of Example 2.

(Unit: wt%) Cosmetic composition for promoting eyebrow and eyelash growth according to Example 2 2.5 Butylene Glycol 5.0 glycerin 5.0 Triethanolamine 0.5 Carbomer 0.2 Tocopheryl acetate 0.1 Methyl paraoxybenzoate 0.3 Pigment Suitable amount ethanol Suitable amount Purified water Balance system 100.0

Formulation Example 2: Preparation of Mascara

To the composition ratio shown in Table 6, to prepare a mascara comprising a composition for promoting eyebrow and eyelash growth of Example 2.

(Unit: wt%) Cosmetic composition for promoting eyebrow and eyelash growth according to Example 2 2.5 Cetyl alcohol 3.5 Beads wax 8.0 Carnauba Wax 3.0 Candelilla wax 1.0 Sorbitan olivate 1.0 kaoline 2.0 Acrylate Copolymer 15.0 FV 1.0 Triethanolamine 3.0 Xanthan gum 0.3 Methyl paraoxybenzoate 0.3 Pigment Suitable amount Purified water Balance system 100.0

As such, the composition according to the embodiment of the present invention is harmless to the human body, and may provide an excellent effect of promoting the growth of eyebrows and eyelashes.

Claims (13)

As a composition for promoting eyebrow and eyelash growth, comprising: jinju green milk crude herbal extract, tissue cultured wild ginseng culture root extract, and tissue cultured geumnyeon extract,
The herbal extract of Jinju green milk milk is characterized in that the mixture of five or more selected from the group consisting of mansiform, guridae, acupuncture, nasturtium, windproof, bokbunja, raw jihwang, Yeongneung, mango, cloves, baekbaek, Chuckhwa and Gazapi Composition for promoting eyebrow and eyelash growth. The method according to claim 1, characterized in that it comprises 9 to 80% by weight of the extract of Jinju green walnut milk herbal extract, 9 to 80% by weight of the cultured wild ginseng root extract and 9 to 80% by weight of the tissue culture gold extract Composition for promoting eyebrow and eyelash growth. delete The method of claim 2, wherein the composition further comprises 0.1 to 30% by weight of the plant complex extract based on the total weight of the composition,
The plant complex extract is a composition for promoting eyebrow and eyelash growth, characterized in that the mixture of one or two selected from the group consisting of iris extract and green tea extract. delete Promoting eyebrow and eyelash growth, comprising 0.1 to 50% by weight of the composition for promoting eyebrow and eyelash growth according to any one of claims 1 to 2 and 4, and 50 to 99.9% by weight of cosmetic excipients For cosmetics. The cosmetic for promoting eyebrow and eyelash growth according to claim 6, wherein the cosmetic is formulated into at least one selected from the group consisting of liquid, spray, gel, paste and emulsion. The method of claim 6, wherein the cosmetic composition is mascara, eyeliner, eye shadow, eyebrow, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nutrition lotion, nutrition cream, essence, nutrition essence, pack Cosmetics for promoting eyebrow and eyelash growth, characterized in that it is formulated into one or more selected from. a) mixing a solvent with each of the Jinju lychee milk herbal mixture, tissue cultured wild ginseng culture root, and tissue cultured geumsunyeon;
b) aging and filtering the respective mixtures obtained in step a), and then preparing each extract to obtain the filtrate; And
c) a composition manufacturing step comprising the extract of Jinju green walnut milk herbal extract obtained in step b), tissue cultured wild ginseng culture root extract, and tissue cultured geumnyeonyeon extract;
The Geumju eunwook crude herbal mixture is an eyebrow and characterized in that the mixture of five or more selected from the group consisting of mansiform, guridae, acupuncture, nasturtium, windproof, bokbunja, raw jihwang, Yeongneung incense, forget-me-not, clove, baekbaek, Chuckhwa and Gazapi Method for producing a composition for promoting eyelash growth. 10. The method of claim 9, further comprising removing the extraction solvent at 50 to 60 ° C. after the b) extract preparation step. 11. 10. The method according to claim 9, wherein the total weight of the composition of the extract of the extract of green medicinal herb extract, 9 to 80% by weight of the cultured wild ginseng root extract and 9 to 80% by weight of the cultured Geumnyeon Ryun extract Method for producing a composition for promoting eyebrow and eyelash growth. The method according to claim 11, eyebrow and eyelash growth further comprises 0.1 to 30% by weight of the plant complex extract which is one or a mixture of two selected from the group consisting of iris extract and green tea extract in step C) Method for producing a composition for promotion. The method of claim 9, wherein the solvent comprises water including purified water; Lower alcohols having 1 to 4 carbon atoms; Hexane; 1,3-butylene glycol; Propylene glycol; Pentylene glycol; glycerin; Acetic acid; ether; And a mixture of one or two or more selected from the group consisting of chloroform.