KR101179609B1 - Method for producing seed pellet of riparian tree, and method of cultivating tree using the same - Google Patents

Abstract

PURPOSE: A method for producing seed pellets of riparian trees and a method for cultivating trees using the same are provided to reduce expense for cultivation and management. CONSTITUTION: A method for producing seed pellets of riparian trees is as follows. Seed pellets of riparian trees are pre-treated by dipping the pellets in a polyethylene glycol or potassium chloride solution. A coating material is manufactured by mixing gypsum, diatomite, dalma ceramic, and Vermiculite. The pre-treated seed pellets of riparian trees is put into the rotary plate of a pelletizer, the coating material and an adhesive are poured into the rotary plate, the seeds are coated, and firstly coated seed pellets are manufactured. The manufactured seed pellets are put into the pelletizer, one or more selected from a group consisting of rheum coreanum extracts and hydrogel are put into the pelletizer, and secondly coated seed pellets are dried.

Description

Method for producing seed pellets and cultivating tree using the same method

The present invention provides a method for producing seed pellets of double coated waterside greenery trees, seed pellets prepared by the above method and seed pellets of the waterside greenery trees using seed pellets of waterside greenery trees, characterized in that the germination. Provide a cultivation method.

Waterside green tree species Seed size is less than 1 mm, unstable germination and hair growth, poor initial growth, different water requirements and require a large amount of water at a specific time, so that vegetation restoration through seeds In the plan, problems such as failure of planting due to competition with weeds and seed loss due to flooding of rivers are occurring.

 In order to restore vegetation, not only herbaceous plants but also planting trees are needed, but the planting of waterside greenery tree species is grown in seedlings and is planted in mainpo or restoring ground when it reaches a certain size for trees over a certain number of years. Growing trees and planting them in the main fabric until the labor of the plant shows the increase of the cost of vegetation restoration due to the increase of the price of the trees and the management cost calculation. Therefore, it is required to develop the cultivation technology that can omit seedling installation and transplanting work. have.

As an alternative method by direct cultivation, the seeds are mixed and sown together to restore them.However, as the herbaceous plants are sown together with the trees, the seeding requirements are high and the seeding density is uneven. After germination, seedling quality is also deteriorated, and it is difficult to restore vegetation by trees reflecting the original ecological type because tree species are suppressed by herbs and weeds.

For this reason, it is necessary to stabilize germination and seedling in pavement for planting tree species by direct wave in vegetation restoration, and shorten the period from seeding to emergence. It is necessary to find a plan and improve the adaptability of the environment through a series of complex cultivation methods, such as promoting early growth of seedlings and preventing stock diseases. In order to solve this problem, many studies have been made to granulate small seed and to increase the appearance rate by shortening the appearance time in the packaging by priming the seed.

In Korean Patent No. 0001087 of the prior literature on the coating method for increasing the activity and growth of the conventional seed, in plant seed pellets, fertilizers, growth regulators, fungicides, insecticides are mixed with peat moss and plant seeds may be inserted. The present invention relates to a method for manufacturing plant seed pellets prepared by molding, drying, pressing and sealing plant seeds after drying in a shape. The plant seed pellets prepared by the above method remove moisture from the pellets to prevent physiological reaction of seeds. After germination of seeds, it provides excellent growth and no cover, which can be applied to plants with low water demand such as marigold, salvia and bulbous plants. Not suitable for waterside green tree species on growth.

In addition, Korean Patent Publication No. 2010-0054704 discloses rice seeds grown in fresh water, which are generated by wind or the like on coated seeds coated with a coating material containing silicic acid and calcined gypsum or silicic acid, iron and calcined gypsum as active ingredients. It does not flow according to the wave (water flow), prevents damage to algae (birds), and has low temperature resistance, growth characteristics of the vertical distribution of rice tissues (leaves, etc.), enhancement of photosynthesis of rice, and plant tissues (leaves, stems, etc.) Silicification has provided a method for producing silicic acid and calcined gypsum or silicic acid, iron and calcined gypsum coated seeds having resistance to disease and doping resistance. It is difficult to apply the seed germination and growth differences between trees to large tree species, and it is necessary to do sowing, not transplanting after germination. It is difficult to apply to waterside green tree species.

As in the above prior document, the coating layer is applied when spraying the electrodeposition agent, phytohormones, phytonutrients, etc. while rotating the wheel drum containing the seed within the range of 10 to 30 rpm without any pretreatment of the seed. It has a thickness of 1 to 6 mm, which is relatively thicker than the seed, so that the seeds are hard to germinate, and there is a problem that the value of the germination may be disproportionated according to the strength of the vitality of the seed object, thereby losing the useful value.

Seed treatment techniques can be roughly divided into priming, coating, pellets, etc. Among them, pellets are small grains, or the size or shape of seeds is not suitable for mechanical sowing, or hand handling. It is a technology to shape the size and shape of seeds using inert materials that do not affect germination of tough seeds.

Priming is used to shorten germination days, improve germination and germination potential, increase seedling production and yield, and not only take into account the type, concentration, treatment duration and temperature of the treatment agent, but also depending on the crop. The effect also appears different.

The main purpose of making pellet seeds is to make sowing easier, but it has the advantages of saving effort and time for sowing and gathering, reducing the scarring of seedlings that may occur when gathering, and saving expensive seeds. Therefore, in the production of seedlings of tree species to be planted in the restoration, the construction of such pellet seeds is a suitable technique that can be applied to the production of seedlings of riparian greenery tree species and restoration of restoration sites.

Pellet seeds should be of sufficient hardness to be sown by machine and should not be affected by the germination, appearance, or seeding of seeds by the coating material. Therefore, the technique of making pellet seeds achieves the desired benefits while minimizing these disadvantages. It is important to explore quality coating materials and to improve the properties of the pellet seeds.

The type of adhesive used for pellet seeds also affects the hardness and seed germination of the pellets depending on the type. The preferred adhesive is water-soluble and easily soluble in water and adheres without affecting the environment such as seeds or water. It must be good.

The purpose of this study is to examine the results obtained by performing empirical tests to reduce the costs required for cultivation and management, to achieve the viability by using pellet seeds, and to make practical use for restoration by seeding and growing seedlings.

The present invention is derived from the above requirements, the present invention achieves the viability for the tree species for cultivating waterfront greenery to reduce the costs required for cultivation and management, seed for the practical use for restoration and seedling growth by direct wave It is to provide a seed pellets by optimizing the pretreatment method and pellet production method of the pellets to produce the seed of the seeds, so that the seeding is easy, the germination rate and the hair growth rate is improved.

In order to solve the above problems, the present invention provides a method for producing a seed pellet of a double coated waterfront greenery trees.

The present invention also provides a seed pellet of waterside greenery trees produced by the above method.

The present invention also provides a method for cultivating waterside greenery trees using seed pellets, which is characterized by seeding and germinating seed pellets of the waterside greenery trees.

The pellet seeds prepared by the method of the present invention not only facilitate sowing, but also improve the survival rate and seedling rate of the seeds, and thus can restore the vegetation through the straight wave of the riparian greenery tree species, thereby reducing the economic burden on the transplant. It is also possible to improve the management of tree species.

1 is a photograph showing a manufacturing process, a schematic diagram and the prepared seed pellets of the seed pellets.
Figure 2 shows the structural formula and content of the anthraquinone compounds contained in rhubarb extract.
Figure 3 shows a picture of the seed pellets of ash and teal.
Figure 4 compares the degree of germination according to the seed pretreatment conditions of (A) ash and (B) teal.
Control: Soak in water
5 is a photograph comparing the degree of germination of the ash seed (bare seed) and pellet seeds. Left: Pellet Seed, Right: Bare Seed
Figure 6 compares the weight of the seedlings after 80 days of growth by pelleting the seed of the pellet according to the seed pretreatment of (A) ash and (B) teal.
Control: Soak in water
Figure 7 is a comparison of the degree of growth after 60 days of growth by seeding the seed (bare seed) and pellet seeds of ash tree in the field.

In order to achieve the object of the present invention,

(a) immersing the waterside greenery tree seed in a polyethylene glycol or potassium chloride (KCl) solution for pretreatment;

(b) mixing the ground gypsum, diatomaceous earth, dalma ceramic and vermiculite to prepare a coating material;

(c) putting the pretreated tree seed of step (a) into a rotating plate of a pellet maker and coating the seed while introducing the prepared coating material and adhesive of step (b) to prepare a seed coated pellet first ; And

(d) putting the seed pellets prepared in step (c) back into the pellet maker and putting the at least one selected from the group consisting of rhubarb extract and hydrogel, and coating the seed pellets prepared by coating It provides a method for producing a seed pellet of waterside greenery trees, characterized in that it comprises the step of drying the seed pellet.

In the method of the present invention, the waterfront greenery tree may be, but not limited to, Fraxinus rhynchophylla Hance or Alnus sibirica Fisch.

In addition, in the method of the present invention, the pre-treatment of step (a) is soaked in seeds -0.8 ~ -1.2 MPa polyethylene glycol solution for 3 to 5 days at 12 ~ 18 ℃ when the tree is ash wood After washing for 10 to 20 minutes in running water and drying for 1 to 3 hours at 30 to 40 ℃, then dried for 1 to 3 days at 10 to 20 ℃ and left for 3 to 5 days at room temperature, preferably seed Was soaked in -1 MPa polyethylene glycol solution at 15 ° C. for 4 days, washed in running water for 15 minutes, dried at 35 ° C. for 2 hours, dried at 15 ° C. for 2 days and left at room temperature for 4 days to pretreatment. can do.

In addition, if the tree is a teal, soak seeds in 120 ~ 180 mM potassium chloride (KCl) solution for 3 to 5 days at 12 ~ 18 ℃ and then washed in running water for 10 to 20 minutes and 1 to 3 at 30 ~ 40 ℃ After drying for a period of time it may be dried for 1 to 3 days at 10-20 ℃ and left for 3 to 5 days at room temperature for pretreatment. Preferably, the seeds are soaked in 150 mM potassium chloride (KCl) solution at 15 ℃ for 4 days After washing for 15 minutes under running water, drying for 2 hours at 35 ℃, it can be dried for 2 days at 15 ℃ and left for 4 days at room temperature can be pretreated.

The pretreatment was performed prior to coating the coating material on the seed surface in order to increase the vitality of the seed and to promote metabolism for the seed to germinate. That is, when the seed surface is crushed or small in size, the surface of the seed pellet is spherically coated so that the coating layer is relatively thick, which impedes the respiration of the seed, so that germination is difficult and metabolism of the embryo is not smooth. The optimum pretreatment condition was found. Under the above conditions, the seeds of ash and teal trees were pretreated and prepared as pellets, and showed good germination rate and germination rate when sowing.

In addition, in the method of the present invention, the coating material of the step (b) is a weight of 5 ~ 7: 0.8 ~ 1.2: 0.8 ~ 1.2: 0.8 ~ 1.2 gypsum, diatomaceous earth, dalma ceramic and vermiculite crushed into 100 ~ 200 mesh It may be prepared by mixing in a ratio, preferably gypsum, diatomaceous earth, dalma ceramic and vermiculite pulverized to 150 mesh may be prepared by mixing in a weight ratio of 6: 1: 1: 1.

The gypsum used as the coating material does not need to apply a separate cover by increasing the weight of the manufactured pellets, heavy seed of the pellets to sink the pellet seeds on the ground surface in the fresh water and to prevent the seed from being lost in the flow of water. Has the advantage. In addition, the diatomaceous earth not only promotes the absorption of inorganic nutrients by enhancing the oxidizing power of the seed root, but also neutralizes the organic acid of the plant root zone to prevent imbalance of nutrient absorption.

In addition, in the method of the present invention, the first coated seed pellets of the step (c) is put into the rotary plate of the pellet maker adjusted to the pre-treated wood seed at a rotational speed of 150 ~ 200 rpm and 50 ~ 70 ° inclination ( The seed coated with the prepared coating material and the adhesive of step b) may be coated to prepare a first coated seed pellet. Preferably, the pretreated wood seed is rotated at a speed of 150 to 200 rpm and a 60 ° inclination. Put the coating material and the adhesive of the prepared pellet maker in the rotating plate of the pellet maker while coating the seed and the primary coated seed pellets can be prepared by drying for 2 to 3 hours at 30 ~ 40 ℃ have.

The adhesive added to prepare the primary coated seed pellets may preferably be an aqueous arabic rubber solution, which is superior to other adhesives in adhesive strength and hardness of the formed pellet seeds compared to other adhesives. The ability to gently absorb moisture was excellent.

In addition, in the method of the present invention, the step (d) is preferably put the dried pellet seed in the rotating plate of the pellet maker adjusted to 150 to 200 rpm and 50 ~ 70 ° inclination rhubarb extract and hydrogel (hydrogel) Double coated pellets prepared by coating the seed pellets may be prepared while adding), and more preferably, a rotating plate of a pellet maker in which dry pellet seeds are adjusted at a rotational speed of 150 to 200 rpm and an inclination of 60 °. To put into the rhubarb extract and hydrogel (hydrogel) while inputting the seed pellets can be prepared double coated pellets prepared by coating.

The rhubarb extract is pulverized 200 ~ 300 g dried rhubarb root and then added 2000-3000 mL of chloroform solution, left for 20 to 28 hours at room temperature, extracted 3 to 5 times, filtered and distilled under reduced pressure to extract 0.2 It may be prepared by diluting to ~ 0.4% by volume, and preferably, 250 g of dried rhubarb roots are pulverized, and 2500 mL of chloroform solution is added thereto, and the mixture is left at room temperature for 24 hours, extracted four times, filtered, and distilled under reduced pressure. The extract can be prepared by diluting to 0.3% by volume. The rhubarb extract contains a rhein, emodin, and physion, which have bactericidal effects, so that the rhubarb extract has a bactericidal effect against plaque disease, which frequently occurs in the seedlings of ash and duck trees. It is done.

In addition, the hydrogel may be preferably a starch grafted cross-linked polyacrylate (SCP), which prevents the pellet-type seeds from absorbing excessive moisture and maintains a certain level of moisture to decompose the ten seeds. It will be a facilitating function.

The present invention also provides seed pellets of waterside greenery trees produced by the above method.

The present invention also provides a method for cultivating waterside greenery trees using seed pellets, which is characterized by seeding and germinating seed pellets of the waterside greenery trees. In the cultivation method of the tree of the present invention, the waterfront greenery tree may be, but not limited to, Fraxinus rhynchophylla Hance or Alnus sibirica Fisch.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

1. Selection of coating material and adhesive for seed pellets

(1) Test Material

Seeds of waterside greenery tree species Ash and Tealwood used for the test were domestic native species (Gangwon Samcheok, Jeonnam Muan) collected in February 2009, and the coating material used for seed pellets can be purchased in Korea. Purchased gypsum, diatomaceous earth, zolite, zirconium silicate, pagodite, bentonite clay, vermiculite (VM), etc., as a homogenizer. After grinding, the powder passed through 150 mesh was used to mix a single coating material and various coating materials. The adhesive used in the pellets was 3% polyvinylalcohol (PVA), 1% CMC (carboxy methylcellulose) and arabic gum ( arabicgum) was prepared using an 8% aqueous solution. The fungicide used in the multi-layer coating used 0.3% of rhubarb extract containing Rhein, emodin, and physion, which have bactericidal effect, and SCPA (starch grafted cross). Pellets were prepared by constructing a multi-layer by applying -linked polyacrylates or alginate at a level of 1.5%.

The rhubarb extract is dried Rheum palmatum ) 250 g of the roots were added 2.5 L of chloroform, and the mixture was left at room temperature for 24 hours, extracted four times, filtered and distilled under reduced pressure to obtain 13.05 g of the extract. The extract was diluted to a level of 0.3%. Prepared.

Starch-grafted cross-linked polyacrylates (SCP) are highly hygroscopic hydrogels derived from nitrile hydrophilic substitution, cyclization and crosslinking with corn starch. It is excellent and not sensitive to pH, so it does not supply pellet moisture or maintains a certain level of moisture even if there is excessive moisture. The preparation of SCPA is carried out by adding 20 g of water-soluble starch to a three-necked flask containing 167 mL of distilled water, attaching a condenser, and heating and dissolving at 95 ° C. under nitrogen for 1 hour to obtain gelatinized water-soluble starch. After the temperature was lowered to 30 ° C., an appropriate amount of acrylonitrile (AN) was added while stirring, and an aqueous CAN solution in which 3 mL of 1N nitric acid was dissolved in 0.338 g Ceric ammonium nitrate (CAN) was added thereto, followed by polymerization at 30 ° C. for 2 hours. It was. After the polymerization was completed, 1N potassium hydroxide was added to make neutral, and then excess methanol was added to precipitate the polymer, filtered through a glass filter, washed with methanol, and dried under reduced pressure at room temperature for 24 hours. Weigh the dry polymer to determine the total polymerization yield of acrylonitrile, and immerse it in DMF (dimethyl formamide-NN, dimethylformamide) to extract, remove and separate sole polyacrylonitrile (PAN). The precipitated copolymer was dried to obtain a graft polymer of pure starch modified polymer. 2 g of SCPA obtained were hydrolyzed with 1N KOH. After hydrolysis it was washed several times with methanol and dried under reduced pressure at 60 ℃ to prepare a hydrogel.

(2) pellet way

The pellet maker used Seed Processing Holland BV (Type 9103.00.00, Netherland), which used the Seed Pelleting Unit to adjust the rotation speed and to adjust the inclination of the rotating plate. The pellet manufacturing method is to adjust the rotation speed of the pellet maker to 150 ~ 200 rpm, the inclination is 60 °, then put the seed on the rotating plate and add a small amount of coating material while spraying the adhesive with a fine nozzle to control the shape and size of the pellet seed Multi-layer pellet preparation was again pelletized in the same manner as the primary pellet preparation, including the coating material and the adhesive. The prepared pellet seeds were sufficiently dried for 2 to 3 hours at a temperature of 30 to 40 ° C., and then sized with a mesh, and then used in this experiment by examining particle sizes with a slide caliper (CD-15C, Mitutoyocorporation, Japan). ). The multilayer seed pellets were applied at a thickness of about 5 times the seed thickness.

(3) Investigation of characteristics of pellet seed

end. Shape Forming Degree

Waterside Greenery Tree Seeds When pellet seeds are made, the appearance is rough (-), pellets are formed, but they are not smooth in shape, rugged or uneven in pellets (+), and they are round, smooth and very good. The degree of shape formation was shown by things (++), and the degree of dehiscence after water absorption was classified into undivided (-), gold-manned (+) and completely cracked (++), and investigated by the lumen. It was.

I. Hardness

The hardness of the prepared waterside greenery tree species pellet seed was expressed by the pressure (kg / cm 2) when it was broken vertically using a fruit hardness tester (FHM-5 type, Japan).

(4) Germination Characteristics of Pellet Seeds of Waterside Greenery Tree Species

end. Germination Rate of Ash and Teal Pellets Seeds

Germination of the teal pellet seeds formed at each treatment was done by putting two layers of paper towels (10 × 10 cm) in a plastic germination box (sigma product, 11 × 11 × 3 cm), putting 50 seeds in each, and 20 mL of distilled water on it. Pour it every time, sowing in 6 repetitions and left in a thermostat at 20 ℃ to check the germination for 10 days every 6 days and then every day from 7 days to 1 day. The germination tax is expressed by the germination rate up to 6 days after sowing by the International Seed Testing Association (IASTA) and the Association of Official Seed Analysists (AOSA) method, and the number of days required to germinate 50% of the final germination rate ( T50) was calculated using the formula of Coolbear et al. (1984).

I. Straight cultivation of pellet seeds

Seed pellets were prepared from ash and teal seed and seed treated with optimized priming conditions, and multi-layer seed pellets containing polymer and natural fungicide (Rhubarb extract) were prepared. It was. Pellet seeds were prepared using a mixture of gypsum + diatomaceous earth + dalmaceramic + vermiculite exhibiting effective pellet shape and hardness, degradability, stable pH and EC values for pellet production. The pellets prepared using a mixture of dalma ceramic + vermiculite were again treated with 0.3% rhubarb extract and SCPA (starch grafted cross-linked polyacrylates) at a level of 1.5% to construct seed pellets composed of two layers. The pellet seed used was 1 grain / pellet, and a diameter of about 2.8 to 3.9 mm was used. One grain was planted on each 104 seedlings by manpower. After seeding, the plants were irrigated at 3 days intervals to help germination and were not irrigated for 80 days after one irrigation to measure the wetting ability and absorbent ability of starch grafted cross-linked polyacrylates. Treatment was placed. Germination and emergence were investigated by the International Seed Testing Association (ISA) and the Association of Official Seed Analysis (AOSA). The growth test was carried out after 30 days and 60 days after germination was confirmed, and the height, number of leaves and leaf area were measured, and biomass was measured after 80 days.

Example  One: rhubarb  Content Analysis of Anthraquinone Compounds in Extracts

The content of the anthraquinone compound of rhubarb extract is shown in Table 1 below. As the anthraquinone, the contents of rhein, emodin and physion were analyzed. The analysis was performed using LC-10AT HPLC system (Shimadzu Instrument Co. LTD, Japan). The column used YMC-Pack ODS-AMA-303 (5 μm, 4.6 × 250 mm ID), and the mobile phase was a mixture of methanol and 0.3% aqueous phosphoric acid (180: 20). It was analyzed by applying. The injection volume of the sample was set to 20 μl, the flow rate was adjusted to 1 ml / min, and the detector was fixed at 254 nm using a SPD-10A UV detector. Used. For quantification, prepare a calibration curve after preparing a standard sample at 10, 50, 100, 500 ppm, and prepare a calibration curve. Substitute the area in y by using the regression curve of the standard material, and find the concentration (x). The content was quantified by making μg / ml.

Rheum palmatum ) Anthraquinone Compound Content in Root Chloroform Extract Sample Anthraquinone Compound (ug / ml) 0.3% Rhubarb Root Chloroform Extract lane Emodin Fish 68.45 ± 1.25 457.26 ± 2.74 47.68 ± 0.89

As a result, it was confirmed that rhubarb extract contained 68.45 ug / ml of rhein, 457.26 ug / ml of emodin, and 47.68 ug / ml of physion. .

Example  2: depending on the coating material Of pellet seeds  Physical and chemical properties

According to the physicochemical properties of the pellet seeds by coating materials (Table 2), zirconium silicate and pagodite used for single use had good pellets, but the surface was very rough and the hardness was 0.24 kg, respectively. Too weak at 0.75 kg and easily broken. Zirconium silicate and pagodite had low EC of pellet leachate, but the pH was around 7.0 and the thermal deterioration of the pellet after moisture absorption was relatively good. Bentonite, diatomaceous earth and gypsum were well formed in the pellets and the surface was smooth, but bentonite, zeolite and diatomaceous earth did not decompose pellets after water absorption, bentonite was very hard at 5.20 kg and diatomite was hard Weak at 0.28 kg. In the case of zeolite, the hardness was also weak, and the pH was too high at 8.6 and EC at 5.1. Gypsum had good pellet formation and surface and very good pellet deterioration after water absorption, but the hardness was too hard at 4.19 kg. Gypsum + diatomaceous earth, gypsum + vermiculite, and gypsum + dharma ceramic showed good thermal deterioration of pellets after pellet formation and water absorption compared to monolithic materials. In the mixed material of gypsum + diatomaceous earth + dalma ceramic + vermiculite, the formation and surface of the pellets were very good, the hardness was 1.58 kg, and the thermal properties of the pellets were very good.

Among the coating materials, pellet seeds made of a single material were generally unsuitable as a coating material because the surface of the pellet was not smooth, the thermal properties were poor, the hardness was too weak or too hard, and the pH and EC of the pellet leachate was unstable. Compared to the single material, the two or more types of mixed materials were more stable in pellet formation and degradability, hardness, pH of the pellet leachate, and EC. Thus, the mixed material was better than the single material as a coating material for pellet seed production. When pellets are prepared by adding 0.3% rhubarb extract and 1.5% SCPA (starch-grafted cross-linked polyacrylates) or alginate to the optimized mixed material, pellets are formed by the addition of polymer. In the case of multi-layer seed pellets, which can be recoated with a disinfectant and a polymer after coating of the mixed material, the pellet shape and hardness, thermal properties, stable pH and EC are similar to those of only the mixed material added. The value of is shown.

Example  3: the seed according to the type of adhesive Pellets  Forming and hardness

The average hardness was 1.36 kg in ARIBA rubber 8% aqueous solution, 1.27 kg in PVA 3% aqueous solution and 1.26 kg in CMC 1%. In particular, the pellet forming ability was excellent.

Example  4: Waterside greenery Tree species  strain Priming  in progress Osmosis ( osmotic Effect by Type and Concentration

The seeds of waterside greenery tree species ash and teal tree used in the test were domestic native species (Gangwon Samcheok, Jeonnam Muan) collected in February 2009, and the priming drugs were Ca (NO ₃ ) ₂ , KNO ₃ , MgSO ₄ , NaNO ₃ , KCl, K ₃ PO ₄ , NH ₄ NO ₃ (manufactured by Sigma) and Polyethylene glycol 6000 (PEG 6000 manufactured by Sigma) were used. The drug concentrations of PEG 6000 were divided into -0.75 Mpa , -1.0 MPa, -1.5 MPa, and -1.75 MPa. The other drugs were 100 mM, 150 mM and 250 mM, respectively. Seed treatments were primed for 4 days in the dark at 15 ℃ and 20 ℃.

 After priming, the seeds were washed in tap water for 15 minutes, dried at 35 ° C. for 2 hours, then further dried at 15 ° C. for 2 days, and allowed to stand at room temperature (18-20 ° C.) for 4 days to achieve moisture equilibrium, and then used as experimental materials.

As a result, the proper priming period was 4 days in both ash and teal, and the germination rate according to the types and concentrations of priming agents showed a large difference between the treatment agents and the concentrations. As for the germination rate after the priming treatment, the ash was the highest at 91 ° C in PEG 6000 concentration of -1.0 MPa at 15 ° C. However, the germination rate of the other trees was lower than that of the unprimed seeds. Low. The germination rate of primed seeds in ash was increased at most temperatures after priming. At 15 ° C and 20 ° C, the germination rate increased from 38.6% and 39.7% before treatment to 56.5% and 64.0%, respectively. The germination rate also increased at most temperatures after priming but, unlike the germination rate, was about 1.2-3.5 times higher at the appropriate temperature range. Optimal priming condition was that PEG 6000 at -1.0 MPa level showed the best germination rate and germination rate, and for Tealwood, KCl 100 mM and PEG -0.75 MPa showed good results. Excellent germination rate and germination rate were shown. In case of ash wood, treatment with PEG 6000 at -1.0 MPa level at 15 ° C for 4 days showed the best priming condition. In case of Alder trees, treatment with KCl 100 mM at 15 ° C for 4 days showed the best priming condition. (FIG. 4). In priming in ash and teal, the germination rate of priming was not improved or decreased compared to non-treatment, except for certain drugs. This is not clear. The accumulation of ions is believed to interfere with metabolism.

Example  5: coating of teal Pellets  Germination Characteristics of Seeds

The germination characteristics of pellet seeds of teal by coating material showed that the germination rate and germination of pellet seeds prepared from monolithic materials such as zirconium silicate, pagodite, bentonite, diatomaceous earth, zeolite, gypsum, etc. The germination rate of the seed was lower than that of the seed from 10% to 10%. In particular, the bentonite had a very low germination rate of 4.7% and germination rate of 2.0%. Germination rates of gypsum + diatomaceous earth and other mixed materials were 31 ~ 38%, which was higher than that of monolithic materials. Germination rate was the same as that of normal seeds. The germination rate and germination rate were similar to those of the original seeds in pellets coated with rhubarb extract (RR) and SCPA (starch grafted cross-linked polyacrylates) added to optimize the coating material for sterilization efficacy and water retention. After priming and pelleting with coating material, pellets coated with rhubarb extract (RR) and starch grafted cross-linked polyacrylates in multiple layers have a germination rate and germination rate more than twice that of the original seeds. The germination rate was similar to that of the original seed. In case of teal, the germination rate and germination rate of pellet seeds made by monolithic material decreased to 1/2 level compared to the original seeds, and the germination rate of the two mixed materials showed higher germination rate than monolithic material, but gypsum + diatomaceous earth + dalma ceramic + It did not reach the level of mixed material of vermiculite. After priming and pelleting with coating material, pellets coated with rhubarb extract (RR) and SCPA (starch grafted cross-linked polyacrylates) in multiple layers showed germination rate and germination rate more than twice that of the original seeds. Table 5, FIG. 5). In addition, the seed pellets of the ash and teal trees developed above were sown and examined for the growth of each tree species. The leaves were grown longer by 2 cm and the number of leaves was more than 2, and the leaf area was also about 13 cm ² wide, indicating that the seed pellets were prepared and sown. In addition, when the seed pellets were applied, the difference in height, leaf number, and leaf area between the individuals was very small. Seed pellets in teal were slightly higher in height than the original seeds, but there was no significant difference. However, on average, more than one seed was grown in seed pellets, and the leaf area was about 7 cm ² wide. In the case of 60 days, this difference gradually decreased but showed a significant difference (Table 6, FIG. 6, FIG. 7).

These results suggest that the developed pellet technology can be effectively used to improve the initial germination rate and initial growth rate of ash and teal as well as to be applied effectively to early growth. Seed pellets may be used to achieve seedlings of riparian greenery species.

Claims (11)

(a) immersing the waterside greenery tree seed in a polyethylene glycol or potassium chloride (KCl) solution for pretreatment;
(b) mixing the ground gypsum, diatomaceous earth, dalma ceramic and vermiculite to prepare a coating material;
(c) putting the pretreated tree seed of step (a) into a rotating plate of a pellet maker and coating the seed while introducing the prepared coating material and adhesive of step (b) to prepare a seed coated pellet first ; And
(d) putting the seed pellets prepared in step (c) back into the pellet maker and putting the at least one selected from the group consisting of rhubarb extract and hydrogel, and coating the seed pellets prepared by coating Method of producing a seed pellet of waterside greenery trees, characterized in that it comprises the step of drying the seed pellets. The method of claim 1, wherein the waterfront greenery is ash tree or teal tree. The method of claim 1 wherein the adhesive is an aqueous gum arabic solution. According to claim 1, The rhubarb extract is pulverized dried rhubarb root 200 ~ 300 g, and then added 2000-3000 mL of chloroform solution and left to stand at room temperature for 20 to 28 hours and then extracted 3 to 5 times and filtered It is an extract obtained by distillation under reduced pressure. The method of claim 1, wherein the hydrogel is starch grafted cross-linked polyacrylate (SCPA). The method of claim 1,
(a) immersing the waterside greenery tree seed in a polyethylene glycol or potassium chloride (KCl) solution for pretreatment;
(b) preparing a coating material by mixing gypsum, diatomaceous earth, dalma ceramic and vermiculite pulverized into 100 to 200 mesh at a weight ratio of 5 to 7: 0.8 to 1.2: 0.8 to 1.2: 0.8 to 1.2;
(c) putting the pretreated tree seed of step (a) into a rotating plate of a pellet maker adjusted to a rotational speed of 150 to 200 rpm and an inclination of 50 to 70 ° and an aqueous coating solution of the rubber and gum arabic prepared in step (b) Coating the seed while putting the prepared primary coated seed pellets; And
(d) the seed pellet prepared in step (c) is placed in a rotating plate of a pellet maker adjusted to a rotation speed of 150 to 200 rpm and an inclination of 50 to 70 °, and rhubarb extract and SCPA (starch grafted cross-linked poly polyacrylate) are added thereto. Method for producing a seed pellet of the waterfront greenery trees, characterized in that it comprises the step of coating the seed pellet prepared by coating the seed pellet, dried for 2 to 3 hours at 30 ~ 40 ℃. (a) Soak the ash seeds in -0.8 ~ -1.2 MPa polyethylene glycol solution at 12 ~ 18 ℃ for 3 ~ 5 days, wash in running water for 10 ~ 20 minutes, and wash at 1 ~ 3 at 30 ~ 40 ℃. Drying for 1 hour and then drying for 1 to 3 days at 10 to 20 ° C., and pretreating by leaving at room temperature for 3 to 5 days;
(b) preparing a coating material by mixing gypsum, diatomaceous earth, dalma ceramic and vermiculite crushed into 100 to 200 mesh at a weight ratio of 5 to 7: 0.8 to 1.2: 0.8 to 1.2: 0.8 to 1.2;
(c) putting the pretreated ash tree of step (a) into a rotating plate of a pellet maker adjusted to a rotational speed of 150 to 200 rpm and a gradient of 50 to 70 ° and arabic rubber prepared in step (b) Coating the seed while introducing an aqueous solution to prepare a first coated seed pellet; And
(d) the seed pellet prepared in step (c) is placed in a rotating plate of a pellet maker adjusted to a rotation speed of 150 to 200 rpm and an inclination of 50 to 70 °, and rhubarb extract and SCPA (starch grafted cross-linked poly polyacrylate) are added thereto. Method for producing a seed pellet of the ash wood, characterized in that it comprises the step of drying the seed coated pellet prepared by coating the seed pellet for 2 to 3 hours at 30 ~ 40 ℃. (a) Soak the teal seed in 120 ~ 180 mM potassium chloride (KCl) solution at 12 ~ 18 ℃ for 3 ~ 5 days, wash in running water for 10 ~ 20 minutes and dry for 1 ~ 3 hours at 30 ~ 40 ℃. Drying for 1 to 3 days at 10-20 ° C., and pre-treating for 3 to 5 days at room temperature;
(b) preparing a coating material by mixing gypsum, diatomaceous earth, dalma ceramic and vermiculite crushed into 100 to 200 mesh at a weight ratio of 5 to 7: 0.8 to 1.2: 0.8 to 1.2: 0.8 to 1.2;
(c) putting the pretreated teal seed of step (a) into a rotating plate of a pellet maker adjusted to a rotational speed of 150 to 200 rpm and a gradient of 50 to 70 ° and arabic rubber prepared in step (b) Coating the seed while introducing an aqueous solution to prepare a first coated seed pellet; And
(d) the seed pellet prepared in step (c) is put into a rotating plate of a pellet maker adjusted to a rotation speed of 150 to 200 rpm and a gradient of 50 to 70 °, and rhubarb extract and SCPA (starch grafted cross-linked polyacrylate) are added thereto. While coating the seed pellet, the method of producing a seed pellet of a teal, characterized in that it comprises the step of drying a double coated seed pellet prepared at 30 ~ 40 ℃ for 2 to 3 hours. Seed pellet produced by the method of any one of claims 1 to 8. A method for cultivating waterside greenery trees using seed pellets, wherein the seed pellets of claim 9 are sown and germinated. The cultivation method according to claim 10, wherein the waterfront greenery tree is ash tree or teal tree.

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