KR101151555B1 - Composition comprising the extract of Acanthopanax koreanum Nakai or the novel compound derived therefrom for preventing baldness and improving hair growth - Google Patents

Abstract

The present invention relates to a composition having a hair loss prevention and hair growth promoting effect containing an extract of the islands of Aeolian Islands or a novel compound isolated therefrom. As the compound acankoreoside J promotes the growth and proliferation of dermal papilla cells, which play an important role in hair growth, and induces the growth phase of hair follicles, it is used as an external skin pharmaceutical composition and cosmetic composition for preventing hair loss and improving hair growth. Can be used.

Isle of Oak Tree, acankoreoside J, hair follicle, hair loss, dermal papilla cells

Description

Composition comprising the extract of Acanthopanax koreanum Nakai or the novel compound derived therefrom for preventing baldness and improving hair growth}

The present invention relates to a composition for preventing hair loss and improving hair growth, which contains an extract of S. aureum or a novel compound isolated therefrom as an active ingredient.

[Reference 1] Elliott K, et al . , Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses., J Invest Dermatol , 113 , pp. 873-877, 1999

[2] Kaufman KD., Androgens and alopecia., Mol Cell Endocrinol , 198 , pp.89-95, 2002

Botchkarev VA., Molecular mechanisms of chemotherapy-induced hair loss., J Investig Dermatol Symp. Proc , 8 , pp. 72-75, 2003

[4] Botchkarev VA., Stress and the hair follicle: Exploring the connections., Am J Pathol , 162 , pp. 709-712, 2003

[Reference 5] Han JH, et al ., Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle., J Dermatol Sci , 34 , pp.91-98, 2004

[Reference 6] Kaufman KD, et al . , Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia., Expert Opin Investig Drugs , 8 , pp. 403-415, 1999

Document 7 Botchkarev VA, et al ., Molecular control of epithelial- mesenchymal interactions during hair follicle cycling., J Investig Dermatol Symp Proc , 8 , pp. 46-55, 2003

[Reference 8] Ozeki M, et al ., In vivo promoted growth of mice hair follicles by the controlled release of growth factors., Biomaterials , 24 , pp.2387-2394, 2003

9 Jg, Stimulation of human hair growth by the recombinant human KGF-2. Biotechnol. Lett , 27 , pp. 749-752, 2005

Document 10 Kamiya T, et al ., Hair follicle elongation in organ culture of skin from newborn and adult mice., Dermatol Sci , 17 , pp.54-60, 1998

[Reference 11] Hibino T, et al ., Role of TGF-β2 in the human hair cycle., J. Dermatol . Sci , 35 , pp.9-18, 2004

Document 12 Moore KA, et al ., Stem cells and their niches. Science , 311 , pp. 1880-1885, 2006

Document 13 Jahoda CA, et al . , Induction of hair growth by implantation of cultured dermal papilla cells., Nature , 311 , pp.560-562, 1984

Document 14 Han DR, et al ., Studies on morphological and chemotaxonomy and seco-triterpene glycoside component of korean Acanthopanax spp., Bull . D .. S. Pharm . Sci . Inst , 11 , pp.1-66, 1994

[Ref. 15] Park Ho-ki, Cutting and Reproduction Method of Prickly Pear, Korean Journal of Medicinal Crop Science, 2 , pp.133-139, 1994

[Reference 16] Kim YH, et al ., Studies on the constituents of Acanthopanax koreanum Nakai (1)., Kor. J. Pharmacogn , 16 , pp. 151-154, 1985

Reference 17 Hahn DR, et al ., A study on the chemical constituents of Acanthopanax koreanum and its pharmacobiological activities., Yakhak Hoeji , 29 , pp.357-361, 1985

Reference 18 Chung BS, et al ., Studies on the constituents of Acanthopanax koreanum Nakai., Kor. J. Pharmacogn , 17 , pp. 62-66, 1986

[Reference 19] Kim YH, et al ., Studies on the chemical constituents of Acanthopanax koreanum , Arch. Pharm . Res , 11 , pp. 159-162, 1988

[Reference 20] Kim YH, et al ., Pimaradine diterpenes from A canthopanax koreanum . J. Nat . Prod , 51 , pp.1080-1082, 1988

[Reference 21] Kim YH, et al ., Diterpene glycoside form Acanthopanax koreanum ., Kor. J. Pharmacogn , 21 , pp. 49-51, 1990

[Reference 22] Choi HS, et al ., Lupane glycosides from the leaves of Acanthopanax koreanum , Chem. Pharm . Bull , 56 , pp. 1613-1616, 2008

Reference 23 Perry LM, et al ., Medicinal Plants of East and Southeast Asia. MIT Press, Cambrige, p. 41, 1980

[24] Filsell W, et al ., Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines a differentiated phenotype., J. Cell Sci , 107 , pp.1761-1772, 1994

[25] Philpott MP, et al ., Cyclical changes in rat vibrissa follicles maintained in in vitro . J Invest Dermatol . 115 , pp. 1152-1155, 2000

Many studies have been conducted to treat hair loss in recent decades, but the cause of hair loss is still unknown. In view of the known hair loss factors, the suppression or reduction of the function of dermal papilla related to hair cycle regulation (Elliott K, et al. , Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses., J Invest Dermatol , 113 , pp.873-877, 1999), aberrant hair cycles due to male hormone action, hair cycles due to decreased blood flow to the scalp Abnormal changes in (Kaufman KD., Androgens and alopecia., Mol) Cell Endocrinol , 198 , pp.89-95, 2002), anticancer agents (Botchkarev VA., Molecular mechanisms of chemotherapy-induced hair loss., J Investig Dermatol Symp Proc , 8 , pp.72-75, 2003), mental stress, physical Botchkarev VA., Stress and the hair follicle: Exploring the connections., Am J Pathol , 162 , pp. 709-712, 2003.

Minoxidil and finasteride are well known as currently approved by the Food and Drug Administration (FDA) for promoting hair growth. Minoxidil was initially developed as a vasodilator for the treatment of hypertension, but it was developed as a hair restorer as hirsutism was reported as a side effect. The mechanism of action of minoxidil on the hair growth effect has not been clarified so far, but the effect of increased nutrient supply through vasodilation, potassium channel opening effect and apoptosis inhibition of dermal papilla cells Etc. are thought to induce hair growth (Han JH, et. al ., Effect of minoxodil on proliferation and apoptosis in dermal papilla cells of human hair follicle., J Dermatol Sci , 34 , pp. 91-98, 2004). Finasteride, developed by Merck, is a substance that inhibits the activity of 5α-reductase, an enzyme that acts on male hormone metabolism, and was developed as a treatment for prostatic hyperplasia, but it is known that it promotes hair growth. Developed as a hair restorer (Kaufman KD, et al . , Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia., Expert Opin Investig Drugs , 8 , pp. 403-415, 1999). Recently, various research institutes are actively researching a number of regulatory factors involved in hair growth and hair loss mechanisms, and in particular, hair growth effects due to signaling by various factors and their receptors related to the hair cycle of growth, degenerative and resting phases. Is constantly being reported. For example EGF and EGFR (Botchkarev VA, et al ., Molecular control of epithelial- mesenchymal interactions during hair follicle cycling., J Investig Dermatol Symp Proc , 8 , pp. 46-55, 2003), VEGF and VEGFR (Ozeki M, et al ., In vivo promoted growth of mice hair follicles by the controlled release of growth factors., Biomaterials , 24 , pp.2387-2394, 2003), HGF / SF and c-MET (Botchkarev VA, et. al ., Molecular control of epithelial-mesenchymal interactions during hair follicle cycling. J Investig Dermatol Symp Proc , 8 , pp. 46-55, 2003), FGF family and FGFR (Jang JH, Stimulation of human hair growth by the recombinant human KGF-2. Biotechnol . Lett , 27 , pp. 749-752, 2005), IGF and IGF-IR (Kamiya T, et al ., Hair follicle elongation in organ culture of skin from newborn and adult mice., Dermatol Sci , 17 , pp.54-60, 1998), TGF-β and TGF-βR (Hibino T, et al ., Role of TGF-β2 in the human hair cycle., J. Dermatol . Sci , 35 , pp.9-18, 2004) and others have been reported to affect the hair cycle by regulating the activity of dermal papilla. In addition, the Wnt pathway and Bmp signaling have been found to be crucial for the proliferation or differentiation of hair follicle stem cells in the bulb region (Moore KA, et. al ., Stem cells and their niches. Science , 311 , pp. 1880-1885, 2006).

Hair growth is caused by a complex mechanism due to the action of several genes, growth factors and their receptors, and systemic hormones. In particular, hair follicle cells composed of epithelial cells and hair papilla cells composed of mesenchymal cells act as pivotal factors for hair formation and growth. Jahoda (Jahoda CA, et al . , Induction of hair growth by implantation of cultured dermal papilla cells., Nature , 311 , pp.560-562, 1984), etc., suggests that hair growth may be transient when hair papilla cells are removed from rat vibrissa follicles. The cells moved from the dermis roots to form hair papilla cells and hair growth continued again. If the lower third of the hair follicles were removed, the dermal papilla cells were formed from the dermis roots and the matrix cells from the outer root roots. Was formed and hair growth continued, but when the lower third of the hair follicles were removed, hair growth stopped. However, after removing more than one-third of the lower part of the hair follicles and transplanting the isolated dermal papilla cells, the growth of the hair continues, and it is thought that the dermal papilla cells play an important role in the hair growth.

Acanthopanax koreanum Nakai) is a deciduous shrub belonging to the araliaceae family and is a Korean specialty plant. There are three varieties of 10 kinds of plants in Korea, including the island of Ogalpi trees (Han DR, et. al ., Studies on morphological and chemotaxonomy and seco-triterpene glycoside component of korean Acanthopanax spp., Bull . D .. S. Pharm . Sci . Inst , 11 , pp. 1-66, 1994). In particular, island oakpi trees are distributed all over Jeju Island below 500 m above sea level. Recently, they are easily cultivated on land such as Cheonan and are easy to reproduce in real life or in cuttings (Park Ho-ki, cutting method of prickly ginseng, Journal of Medicinal Crop Science, 2 , pp. 133-139, 1994). To date, the roots and stems of the island oak tree are lignan glycosides, siringaresinol diglycoside (Kim YH, et. al ., Studies on the constituents of Acanthopanax koreanum Nakai (1)., Kor . J. Pharmacogn , 16 , pp. 151-154, 1985), acanthoside D, syringoside (Hahn DR, et. al ., A study on the chemical constituents of Acanthopanax koreanum and its pharmacobiological activities., Yakhak Hoeji , 29 , pp.357-361, 1985), eleutheroside B and E (Chung BS, et al ., Studies on the constituents of Acanthopanax koreanum Nakai., Kor . J. Pharmacogn , 17 , pp. 62-66, 1986), falcarindol, methyl n-hexacosanoate, coniferin (Kim YH, et. al ., Studies on the chemical constituents of Acanthopanax koreanum , Arch . Pharm . Res , 11 , pp. 159-162, 1988), pimaradine diterpene (Kim YH, et. al ., Pimaradine diterpenes from A canthopanax koreanum . J. Nat . Prod , 51 , pp. 1080-1082, 1988) and sumogaside (Kim YH, et. al ., Diterpene glycoside form Acanthopanax koreanum ., Kor . J. Pharmacogn , 21 , pp. 49-51, 1990), lupane glycosides (Choi HS, et. al ., Lupane glycosides from the leaves of Acanthopanax koreanum , Chem . Pharm . Bull , 56 , pp. 1613-1616, 2008). Island oak bark is known to be effective in tonic, rheumatism, diabetes and liver disease (Perry LM, et. al ., Medicinal Plants of East and Southeast Asia. MIT Press, Cambrige, p.41, 1980), there are few studies and reports on the hair growth and hair growth efficacy of S. aureus extract.

Therefore, the present inventors have continuously studied the hair loss prevention and hair growth improvement effect by the extract of S. agakorpi extract or a novel compound isolated therefrom, which plays an important role in the growth effect of vibrissa follicles and hair growth of rats. By confirming the growth proliferation effect of the dermal papilla cells (Dermal papilla cells) to complete the present invention.

In order to achieve the above object, the present invention provides a skin external pharmaceutical composition for preventing hair loss and improving hair growth, containing an acankoreoside J of the following structural formula (I) isolated from an extract of S. aureus.

(Ⅰ)

(Ⅰ)

In addition, the present invention provides a cosmetic composition for preventing hair loss and improving hair growth, which contains an extract of S. augkorpi tree or a novel compound isolated from the acankoreoside J as an active ingredient.

The extract as defined herein is characterized in that it is obtained from the stem, root or leaf, preferably the stem or leaf of the island of the islands.

The extract as defined herein includes extracts available in water, spirits, C ₁ to C ₄ lower alcohols or mixed solvents thereof, preferably water, alcohols, methanol or mixed solvents thereof, including purified water.

The external skin pharmaceutical composition includes a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation.

In addition, the cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer cream, hair massage cream, hair wax, Hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drier, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer Formulations of risers, hair mousses or hairsprays.

Hereinafter, the method of obtaining the extract and the novel compound of the present invention will be described in detail.

The extract of the present invention, after sectioning each of the stems, roots and leaves of the island of Dalpipi by air drying, is about 0.01 to 10 times (v / w), preferably about 0.1 to 3 times (v / w) of the dried sample weight w) water, alcohol, lower alcohols of C ₁ to C ₄ or a mixed solvent thereof, preferably water, alcohol or a mixed solvent thereof, containing a quantity of purified water, and about 0.5 to 24 hours at a temperature of 90 ° C. or higher, Preferably 1 to 12 hours cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heating extraction, and the like, preferably by hot water extraction method is repeated 1 to 5 times, preferably 2 to 3 times and extracted and filtered The extract of the present invention can be obtained through a manufacturing process of concentration under reduced pressure and lyophilization.

In addition, after drying and cutting off the leaves of the leaves of S. aureus, about 0.5 to 50 times (v / w) of methanol is added to the dried sample weight to 1 to 0 ° C to 100 ° C, preferably 30 ° C to 50 ° C. Cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heating extraction for 10 to 10 hours, preferably 1 to 5 times, preferably 2 to 3 times repeated extraction and filtration by hot water extraction method, concentrated under reduced pressure A first step of obtaining methanol extract; Distilled water and hexane (Hexane) was added to the methanol extract, and the mixture was shaken and fractionated into a hexane layer and an aqueous layer. Then, ethyl acetate was added to the aqueous layer, shaken, divided into an ethyl acetate layer and an aqueous layer, and the ethyl acetate layer and the aqueous layer were concentrated under reduced pressure, respectively. A second step of obtaining an ethyl acetate fraction and a water soluble fraction; The water soluble fraction was loaded onto a Diaion HP-20 column, followed by a solvent gradient (100% water, 90% methanol, 75% methanol, 50% methanol, 20% methanol, 100% methanol) with water and methanol as mobile phases. The compound of the present invention can be separated and purified through the third step of preparing 50% methanol fraction obtained by eluting with silica gel and YMC RP-18 column chromatography.

The composition for preventing hair loss and improving hair growth of the present invention comprises 0.1 to 50% by weight of the extract or compound based on the total weight of the composition.

However, the composition is not limited thereto, and may vary depending on the condition and progress of the patient.

The skin external pharmaceutical composition containing an extract of the island of the present invention or a novel compound separated therefrom as an active ingredient is a skin external preparation for preventing hair loss and improving hair growth, such as creams, gels, patches, sprays, ointments, warning agents, lotions, It may be prepared and used as a pharmaceutical composition in the form of an external preparation for skin, such as linen, pasta, or cataplasma, but is not limited thereto.

Preferred dosages of the extracts or compounds of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract or compound of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 10 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

In addition, it may be variously used in hair cosmetics having the effect of preventing hair loss and activating hair growth, containing an extract of the island of the island of the present invention or a novel compound separated therefrom as an active ingredient. Examples of products to which the composition can be added include, for example, hair tonics, hair conditioners, hair essences, hair lotions, hair nutrition lotions, hair shampoos, hair rinses, hair treatments, hair creams, hair nutrition creams, hair moisturizers. Cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair colorant, hair wave agent, hair bleach, hair gel, hair glaze, Cosmetics such as hair dressers, hair lacquers, hair moisturizers, hair mousses or hair sprays and the like.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. And salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be incorporated into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

As animal or vegetable fats and oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE / POP (Polyoxyethylene / Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

Examples of cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium, behenyltrimethylammonium bromide, and chlorides. Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, and N-alpha- hardened fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01 to 5% weight percentage with respect to a total weight, More Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract or compound as an active ingredient, for example, such as stabilizers, solubilizers, vitamins, pigments and flavorings Phosphorus aids and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Since the island extract of the present invention, or a new compound acankoreoside J isolated from it shows the growth proliferation effect of dermal papilla cells that play an important role in hair growth, hair loss prevention and hair growth pharmaceutical composition for improving hair growth And cosmetic compositions.

Hereinafter, the present invention will be described in detail by reference examples, examples and experimental examples.

However, the following Reference Examples, Examples, and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Reference Examples, Examples, and Experimental Examples.

Example  1. Preparation of Pleurotus eryngii Extract

1-1. Preparation of Stem Extract

Island taken from the island senticosus tree plantations senticosus wood (Acanthopanax The stems of koreanum ) were naturally dried and then sliced into 100 g of 1 L 100% alcohol, 50% alcohol and distilled water, respectively, and extracted twice over 3 hours at a temperature of 90 ° C. or higher. The extract was filtered with filter paper (Whatman), concentrated under reduced pressure (Eyela, Rotary Evaporator N-1000) and freeze-dried (Eyela, Freeze Dryer FDU-540) to obtain 8.7 g of 100% alcohol extract (AS1) from the stem. : 8.7%), 50% alcohol extract (AS2) 11.5 g (yield: 11.5%) of the stem and 18.5 g (yield: 18.5%) of the water extract (AS3) of the stem were obtained and used as a sample of the following experimental example.

1-2. Preparation of Citrus Root Extract

Island taken from the island senticosus tree plantations senticosus wood (Acanthopanax Koreanum ) 100 g of alcoholic extract (AR1) 8.3 g (yield: 8.3%), 50% of the roots were subjected to the same process as in Example 1-1, except that the roots of the Koreanum were naturally dried and then sliced . 12.5 g (yield: 12.5%) of alcohol extract (AR2) and 16.5 g (yield: 16.5%) of water extract (AR3) of root were obtained, and used as a sample of the following experimental example.

1-3. Preparation of Scorpion Leaf Extract

Island taken from the island senticosus tree plantations senticosus wood (Acanthopanax Koreanum ) 100 g of alcohol extract (AL1) 8.2 g (yield: 8.2%), 50% of the leaves were subjected to the same process as in Example 1-1, except that the leaves of the Koreanum were dried and then sliced . 13.5 g (yield: 13.5%) of alcohol extract (AL2) and 26.2 g (yield: 26.2%) of water extract (AL3) of the leaves were obtained and used as samples in the following experimental example.

Example  2. New Compound acankoreoside  Separation of J

2-1. From the leaves of the island AK -10 Separation Purification

Island taken from the island senticosus tree plantations senticosus wood (Acanthopanax 3.5 kg of the leaves of koreanum ) were dried and chopped , and then extracted with hot water three times for 5 hours with 9 L of 100% methanol at 60 ° C., and the extract was filtered under reduced pressure to give about 550 g of methanol extract. 1 L of distilled water and 1.5 L of hexane were added to 540 g of the obtained methanol extract, followed by shaking. The mixture was partitioned into a hexane layer and an aqueous layer. The hexane layer was concentrated under reduced pressure to obtain 54 g of a hexane fraction. 1.5 L of ethyl acetate was added to 1.5 L of aqueous layer, shaken, and divided into an ethyl acetate layer and an aqueous layer. The ethyl acetate layer and the aqueous layer were concentrated under reduced pressure, respectively, to obtain 91.3 g of ethyl acetate fraction and 403.7 g of a water-soluble fraction. The obtained water-soluble fraction was loaded onto a Diaion HP-20 column, and then a solvent gradient (100% water, 90% methanol, 75% methanol, 50% methanol, 20% methanol, 100% methanol) with water and methanol as mobile phases. Eluted. Silica gel and YMC RP-18 column chromatography were repeatedly performed on 30.0 g of the obtained 50% methanol fraction to obtain 70 mg of white amorphous powder (AK-10).

2-2. AK -10 structure identification

As a result of measuring the MS spectrum (AGILENT, 1200 SERIES LC-MSD Trap spectrometer) of AK-10 obtained in Example 2-1, it showed a molecular ion peak at m / z 1034.5, HR- By measuring the MS spectrum, the molecular formula could be estimated as C ₅₀ H ₈₂ O ₂₂ . ^{Through the 1} H-NMR, ¹³ C-NMR, DEPT and HMBC spectra, AK-10 is the first new substance to be separated from nature and identified as a novel compound that is a triterpenoid glycoside of the lupane type. And named it acankoreoside J. In Table 1 acankoreoside J [3 α, 20,29 -trihydroxylupan-23,28-dioic acid 28- O - {α -L-rhamnopyranosyl- (1 → 4) - β -D-glucopyranosyl- (1 → 6) - β -D-glucopyranosyl} ester] exhibited the following physical data of the.

No. δH δC DEPT 135 HMBC (H-C) One 1.40 34.23 CH ₂ 2 1.53 26.10 CH ₂ 3 3.67 (m) * 73.37 C1, C5, C24, C1a 4 - 52.13 - 5 1.85 46.93 CH C4, C6, C24, C24, C25 6 22.28 CH ₂ 7 1.31, 1.57 35.52 CH ₂ C5, C9 8 - 43.04 - 9 1.52 52.03 CH 10 38.15 11 22.48 CH ₂ 12 2.20 29.88 CH _-2 13 2.34 39.50 C14 14 - 44.58 15 1.17, 1.62 31.24 CH ₂ C17 16 1.46, 2.35 33.18 CH ₂ C28 17 - 60.12 18 1.74 49.51 C17, C19,20, C28 19 2.37 45.72 CH C18, C20, C30 21 2.1 28.89 CH ₂ C20 22 1.39, 1.81 37.50 C17 23 184.29 - 24 1.09, 3H, s 18.45 CH ₃ C3, C4, C5, C23 25 0.90, 3H, s 17.44 CH ₃ C1, C5, C9, C10 26 0.96, 3H, s 17.29 CH ₃ C7, C8, C14 27 1.07, 3H, s 15.62 CH ₃ C8, 14 28 177.02 - 29 1.04, 3H, s 19.66 CH _-3 C19, C20, C30 30 3.37 (m) * 71.23 C19, C20, C29 1a 3.56 (m) * 73.62 CH ₂ 2a 3.67 62.35 CH ₂ Glc One 5.43, 1H, d, 8.5 95.44 CH C28, C3, C5 2 3.33 (m) * 73.98 CH C1, C2 3 3.43 (m) * 78.33 CH 4 3.42 (m) * 71.14 CH 5 3.43 (m) * 78.21 CH 6 3.78, 1 H, d, 5.0 69.66 CH ₂ C4, C5 4.101H, 1H, d, 10.5 Glc ' One' 4.39, 1H, d, 8.0 104.61 CH C6, C5 ' 2' 3.24, 1H, t, 8.0 75.43 CH C1, C3 3 ' 3.46 (m) 76.81 CH C2, C4 4' 3.54, 1H, t 79.68 CH C3 ', C5' 5 ' 3.29 (m) * 76.99 CH 6 ' 3.66, 3.82 62.02 CH ₂ C4 ', C5' Rha One 4.84, 1 H, d, 9.0 103.03 CH C2 ", C5" 2 3.64 (m) * 72.27 CH 3 3.83 (m) * 72.55 CH C2, C4 4 3.40 (m) * 73.84 CH C5 ", C6" 5 3.96, 1H, dd, 5.5, 8.5 70.78 CH C4 ", C6" 6 1.27, 3H, d, 6.0 18.00 CH ₃ C4 ", C5"

Reference Example 1. Statistical Analysis

All results were expressed as mean ± standard deviation. Statistical significance test was performed by student's t test, and the significance was recognized when p-value was less than 0.05. For statistical processing, SPSS 12.0K for Windows (Release 12.0.1. SPSS Inc. USA) was used.

Experimental Example 1. Measurement of the proliferation effect of dermal papilla cells

Immortalized dermal papilla cells (rat vibrissa immortalized dermal papilla cell) in order to confirm whether or not the extract of the island of the islands obtained in the above example or the new compound acankoreoside J isolated therefrom has a proliferative effect on dermal papilla cells that play a very important role in hair growth (Han JH, et al ., Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle., J Dermatol Sci , 34 ,). pp. 91-98, 2004).

Rat vibrissa immortalized dermal papilla cells; Filsell W, et al ., Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines a differentiated phenotype., J. Cell Sci , 107 , pp. 1761-1772, 1994) with 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated FBS (heat DMEM (Hyclone Inc, USA) medium containing -inactivated fetal bovine serum; Gibco Inc, NY, USA) was incubated in a 37 ° C., 5% CO ₂ incubator and passaged once every 3 days.

The cultured dermal papilla cells (1.0 × 10 ⁴ cells / ml) were put in a 96-well plate and incubated for 24 hours, then exchanged with serum-free DMEM medium for 24 hours, and then the islands obtained in Example 1 above. Agalophyllum extract AS1, AS2, AS3, AR1, AR2, AR3, AL1, AL2 and AL3 were treated at concentrations of 0.1 and 1 ug / ml, respectively, and the pure compound AK-10 isolated and purified in Example 2 was 0.1 And a concentration of 1 uM. In addition, the minoxidil sulfate (minoxidil sulfate; Sigma, MO, USA) as a positive control was treated at a concentration of 1 μM. After incubation for 4 days, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. The supernatant was removed, 200 μl of DMSO was added to dissolve the precipitate, and the absorbance was measured at 540 nm using a microplate reader (Amersham Pharmacia Biotech, NY, USA). The average absorbance value for each sample group was obtained, and the degree of proliferation was investigated by comparing with the absorbance values of the control group.

Experimental results, as shown in Table 2, Table 3, Figure 1 and Figure 2, the extract of the islands of the present invention AS2 (1 ug / ml), AL1 (0.1 ug / ml) and a novel compound AK-10 (1 uM) was significantly increased to 124.3 ± 10.3%, 117.7 ± 7.1% and 121.3 ± 6.5% ( P > 0.05) compared with the control effect (100%). 1 ug / ml), AL1 (0.1 ug / ml) and new compound AK-10 (1 uM) proliferation effect of dermal papilla cells (minoxidil sulfate (MS), a well-known hair growth promoting drug It was confirmed that the proliferation effect of (115.8 ± 10.2%) is more excellent.

Growth rate
(%) AS1 AS2 AS3 AR1 AR2 AR3 AL1 AL2 AL3 MS
(1 uM) 0.1ug / ml 104.8 ± 10.8 113.6 ± 12.0 104.7 ± 6.9 103.7 ± 11.4 97.7 ± 7.4 111.3 ± 12.08 117.7 ± 7.1 * 115.0 ± 8.4 116.4 ± 7.4 115.8 ± 10.2 1ug / ml 108.4 ± 8.6 124.3 ± 10.3 * 106.8 ± 4.9 111.2 ± 13.0 99.1 ± 4.8 113.4 ± 7.8 94.6 ± 9.3 115.5 ± 11.9 112.7 ± 9.5 Comparison with no control group (100%)

Proliferation rate (%) acankoreoside J (AK-10) M.S. 0.1 uM 97.2 ± 5.5 1 uM 121.3 ± 6.5 * 115.8 ± 10.2 Comparison with no control group (100%)

Experimental Example  2. Mustache hair follicles ( Vibrissa follicles ) Growth effect measurement

2-1. Rat  Mustache hair follicles ( Rat vibrissa follicles Isolation and Cultivation

In order to measure the hair growth efficacy of AK-10, a novel compound of Example 2, the following experiment was performed using the method described in the literature (Philpott MP, et. al ., Cyclical changes in rat vibrissa follicles maintained in in vitro . J Invest Dermatol . 115 , pp. 1152-1155, 2000).

Three-week-old male Wistar rats (Japan SLC, Hamamatsu, Janpan) were purchased from a central laboratory animal, anesthetized with ethyl ether and cervical slaughtered. Rat left and right mystacial pads were isolated to isolate E / P buffer [(EBSS, Earle's balanced salts solution, Sigma MO) containing 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA). , USA) + (PBS, phosphate-buffered saline, Sigma MO, USA)]. The vibrissa follicles were carefully separated while observing under a dissecting microscope. Until the hair follicles were separated, the separated hair follicles were placed in a Petri dish containing E / P buffer and incubated at 37 ° C. in a 5% CO ₂ thermostat for about 1 hour. In each well of a 24-well plate 2 mM L-glutamine (L-glutamine, Gibco Inc, NY, USA), 10 μg / ml insulin (insulin, Sigma MO, USA), 50 nM hydrocortisone (Sigma MO, USA) and 500 μl of William E medium (William E medium, Gibco Inc, NY, USA) containing 100 units / ml penicillin-100 μg / ml streptomycin and put one follicle into one well at 37 ° C. , 5% CO ₂ incubator. 10-12 hair follicles were used in one experimental group, and the medium was changed every 3 days during the culture. AK-10, a novel compound of Example 2, was treated at concentrations of 0.1, 1, and 10 uM, and minoxidil sulfate (minoxidil sulfate, MS, Sigma, USA), a positive control, was treated at a concentration of 1 μM.

2-2. Mustache hair follicles ( Rat vibrissa follicles ) Growth measurement

The morphology of the cultured mustache follicle (vibrissa follicle) was photographed using a microscope (Olympus, Japan). Hair follicle length was measured on day 0, 7, 14 and 21 using an image analyzer (DP controller; Olympus, Japan). The average value of the hair follicle length change was calculated and compared with the average length of the control group.

2-3. Experiment result

As a result, the growth of the three-week-old rat growth period mustache follicles (vibrissa follicles) was isolated and cultured for three weeks to determine the hair follicle efficacy of the novel compound AK-10 of Example 2 by measuring the hair follicle length. As shown in FIG. 3, a comparison of the length difference (%) of hair follicles between the AK-10 treated group and the control group showed that when AK-10 was treated at a concentration of 0.1 uM, the hair fibers were observed on the 21st day. The growth effect was significantly increased to 118.1 ± 12.0% ( P > 0.05) than the control group (100%), and the positive control group, 1 uM minoxidil sulphate (MS), showed 112.9 ± 10.2% growth effect.

Hereinafter, the preparation examples of the composition comprising the extract or the compound of the present invention, but the present invention is not intended to limit it, only intended to explain in detail.

Formulation example  1. Preparation of Hair Tonic

Resorcinol 0.1%

Menthol 0.05%

Panthenol 0.2%

Salicylic Acid 0.1%

Tocopheryl Acetate 0.1%

Pyridoxine hydrochloride 0.1%

Castor Oil 5.0%

AS2 (50% spirit extract of island stems) 10.0%

Pigment amount

Spices

Ethanol

Purified water level

Total 100.0%

Formulation example  2. Of hair conditioner  Produce

Cetanol 3.5%

Self-emulsifying glycerin monostearate 1.5%

Propylene Glycol 2.5%

Stearylmethylbenzyl Ammonium Chloride (25%) 7.0%

0.3% methyl paraoxybenzoate

AL1 (100% spirit extract of island ivy leaf) 2.5%

Pigment amount

Spices

Purified water level

Total 100.0%

Formulation example  3. Hair lotion  Produce

Resorcinol 2.0%

Menthol 2.0%

Panthenol 0.5%

Pyroctonolamine 0.1%

AL2 (50% alcohol extract of the leaves of the island of Oak) 5.0%

Fragrance, pigment 0.5%

Purified water level

Total 100.0%

Formulation example  4. Hair soap  Produce

AL3 (water extract of Scorpio alba leaves) 0.1%

Titanium dioxide 0.2%

Polyethylene Glycol 0.8%

Glycerin 0.5%

Ethylenediaminetetraacetic Acid 0.05%

Sodium 1.0%

Pigment amount

Soap flavor

Cosmetic soap base (13% moisture, parts by weight)

Total 100.0%

Formulation example  5. Hydrophilic Ointment

number Raw material Content (% by weight) One   AK-10 (Example 2) 0.5 2   White vaseline 250 3   Stearyl alcohol 220 4   Ethyl (or methyl) p-oxybenzoate 0.25 5   Propylene glycol 120 6   Sodium lauryl sulfate 15 7   Propyl p-oxybenzoate 0.15

1 is a diagram showing the dermal papilla cell proliferation effect of the extract of S. aureus bark obtained in Example 1 (bar:% of control, broken line:% of positive control minoxidil sulfate),

Figure 2 is a diagram showing the effect of proliferation of dermal papilla cells of the novel compound (AK-10) isolated and purified in Example 2 (bar:% of control, broken line:% of positive control minoxidil sulfate),

3 is a diagram showing the growth effect of vibrissa follicles of the novel compound (AK-10) isolated and purified in Example 2 (% of control).

Claims (7)

Papillary alopecia, alopecia areata, alopecia areata, traumatic alopecia, traumatic alopecia, scarring alopecia containing an extract extracted from an isolaris bark tree with a mixed solvent of water and alcohol or an acankoreoside J of the following structural formula (I) as an active ingredient Or external dermatological pharmaceutical composition for preventing and improving alopecia areata

(Ⅰ) According to claim 1, wherein the extract is an external skin pharmaceutical composition obtained from the stem, root or leaves of the island. delete The external skin pharmaceutical composition according to claim 1, wherein the external skin pharmaceutical composition is a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation. delete Resting alopecia, alopecia areata, traumatic alopecia, traumatic alopecia, traumatic alopecia, scarring or nasal scar, which contain an extract extracted from an isolaris bark tree with a mixed solvent of water and alcohol or the isolated novel compound acankoreoside J of claim 1 as an active ingredient. Cosmetic composition for preventing and preventing hair loss. The method of claim 6, wherein the cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer cream, hair massage Cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave, hair bleach, hair gel, hair glaze, hair dresser, A cosmetic composition in the form of a hair lacquer, a hair moisturizer, a hair mousse or a hairspray.

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