KR20060031007A - Cosmetic composition comprising the extract of gastrodia elata blume and the compound isolated therefrom having skin whitening effect - Google Patents

Abstract

The present invention relates to a cosmetic composition for whitening, and more particularly to a cosmetic composition containing Gastrodia elata BLUME extract having a skin whitening effect and a compound separated therefrom.

The composition of the present invention effectively inhibits the production of melanin by tyrosinase activity and melanoma B16, which are enzymes essential for melanin biosynthesis, and thus can be usefully used as a cosmetic composition for preventing skin aging and whitening.

Cheonma, skin whitening, cosmetics

Description

Cosmetic composition comprising the cheonma extract having a skin whitening effect and a compound isolated therefrom {Cosmetic composition comprising the extract of Gastrodia elata BLUME and the compound isolated there from having skin whitening effect}

The present invention relates to a cosmetic composition for skin whitening containing the cheonma extract and a compound isolated therefrom.

Melanogenesis is a defense mechanism against melanocyte pigmentation cells (melanocytes) in the skin cells, which increases the melanogenesis activity, and the large amount of melanin produced by this transfers to the keratinocytes. It is the result of accumulation in the epidermis. Although melanin protects the skin, hyperpigmentation of the skin causes blemishes, freckles, darkening of the skin after skin inflammation, and senile pigment spots. (M. Kubo, and H. Matsuda, Fragrance Journal , 8 , 48, 1995, TP Dooley, RC Gadwood, K. Kilgore, and LM Thomasco, Skin Pharm. , 7 , 188, 1994). Therefore, recently, various whitening cosmetics and medicines for preventing and improving such problems have been developed and marketed.

Specifically, melanin is produced by tyrosine through enzyme and non-enzymatic oxidation in melanocytes (melanocytes) in the basal layer of the skin epidermis, and is transferred to keratinocytes constituting the epidermis. Important enzymes involved in the biosynthesis of melanin include tyrosinase, tyrosinase-related protein 1 and dopachrome tautomerase. Two enzymes besides tyrosinase have been recently developed and attracting attention recently, but the enzymes that play a decisive role in melanin synthesis are tyrosinase (M. Seiji, K. Shimao, MS Birbeck, and TB Fitzpatrick, Ann. NY Acad. Sci. , 100 , 497, 1963). The first stage of biosynthesis of melanin is caused by tyrosinase. Tyrosinase causes tyrosine to be dopa (Dopa), which makes it dopaquinone (Dopa-quinone). The next steps in these two reactions are known to be possible by non-enzymatic reactions, and tyrosinase alone is known to produce melanin (P. Bernard, and JY Berthon, International Journal of Cosmetic Science , 22 , 2219, 2000). .

Recently, a new theory of melanin formation following the expression of signal transduction agents in skin cells has been proposed. That is, when the skin is exposed to ultraviolet light, interlukin-α, which causes inflammation, is expressed from keratinocytes in the epidermis, and this interleukin-alpha is keratinocyte endothelin. Promotes expression. This series of processes increases the formation of melanocytes and activates melanocytes. In the melanosomes formed in this manner, tyrosine forms melanin by tyrosinase (T. Cohen, RM Muller, Y. Tomita, and S. Shibahara, Nucleic Acids Res , 18 , 2807, 1990, K. Tsukamoto, IJ Jackson , K. Urabe, PM Montague, and VJ Hearing, EMBO J. , 11 , 519, 1992).

Ascorbic acid (Japanese Patent Laid-Open Publication No. Hei 4-9320), hydroquinone (Japanese Patent Laid-Open Publication No. Hei 6-192062), Kojisan (Japanese Patent Laid-Open Publication No. 56-7710), Arbutin (Japanese Patent Laid-Open Publication No. 4-9315) And some of the compounds have tyrosinase inhibitory activity and are used as raw materials for whitening cosmetics, but due to poor stability in the prescription system, they are degraded due to poor coloration, odor occurrence, efficacy at the biological level, unclear effect and safety issues. The use is limited by such a situation. And the inhibitory effect of tyrosinase has been demonstrated, but the effect is low in experiments similar to the actual biologic level. Therefore, the inhibitory effect of the melanoma cell culture similar to the biological level is required. Hydroquinone is also regulated as a carcinogenic substance and is prohibited from use. Kojic acid and ascorbic acid are very unstable substances. Browning can occur when stored in small amounts for several weeks at room temperature.

Therefore, in order to develop a whitening cosmetics having relatively low toxicity and nature-friendly, studies have been conducted to search for and use herbal medicines having whitening effects. In other words, in order to solve the problem of raw materials with a whitening effect by suppressing melanin production for a long time, various melanin production can be suppressed from plant extracts having excellent safety, such as herbal medicines, vegetables, fruits and flowers, which are used as herbal medicines. Efforts have been made to find extracts with different effects.

Specifically, whitening cosmetics containing a mulberry extract was disclosed in Korean Patent Publication No. 1991-8660, whitening cosmetics containing Hojang-geun extract in Korean Patent Publication No. 1991-11645, Korean Patent Publication No. No. 1993-28783 discloses a whitening composition containing an extract of lettuce extract, and recently, a whitening cosmetic containing melberin isolated from mulberry is disclosed in Korean Patent Publication No. 1997-47260.

Chun Ma (Gastrodia elata BLUME) is to be dried rhizome of perennial parasitic plants of thousand miles (Gastrodia elata BLUME) belonging to the Orchidaceae (Orchdaceae), the pyeonap columnar-waterproof type, the surface is irregular longitudinal wrinkles and turning bars in fawn have. Produced in Gangwon, Gyeonggi-do, China, Japan, etc., there are natural and artificial cultured herbs. Chunma has been used for centuries in oriental medicine as a traditional herbal medicine, as an antiepileptic agent, as a stabilizing agent for pain and dizziness, hypertension, general paralysis and tetanus. Active ingredients include gastrodin, phenolic compounds, organic acids, sugars, and β-sitosterol (Jung Bo-seop and Shin Mingyo, Pharmacological Dictionary, Younglimsa, pp 138-139, 1998). Vanilly alcohol and gastrodine are known to have antiepileptic effects, and recently, constituents of Chunma have been reported to inhibit glutamate-induced apoptosis in neurons (YS Lee, et al., Arch. Pharm. Res. , 22 , p404, 1999). In addition, the ester fraction of Chunma reduces the decrease of γ-Aminobutylic acid (GABA), decreases the increase in glutamate content, and has an antiepileptic effect in pentylene tetrazole-induced epilepsy. Has been reported (JH Ha, et al., J. Ethnopharmacol , 73 , p329, 2000).

To date, dementia prevention and treatment composition using cheonma extract (Korea Patent Publication No. 10-0360674-0000, 2002/10/29), Composition having anti-cancer and cancer metastasis effect (Korea Patent Publication No. 10-0425978-0000 2004 / 03/24), but also related to the extract of cheonma, such as health food composition for promoting blood circulation (Korea Patent Publication No. 10-0376306-0000, 2003/03/04), but such technology does not disclose skin whitening effect There is no research on the active ingredient.

Accordingly, the present inventors studied herbal extracts to find a plant-natural substance that is effective for skin whitening. As a result, the cheon-ma extract of the present invention and 4-hydroxybenzyl alcohol and gastrodine (Gastrodin) isolated therefrom The present invention was completed by confirming that) inhibits the activity of tyrosinase and inhibits melanin production of melanoma B16.

Accordingly, it is an object of the present invention to provide a cosmetic composition comprising a cheonma extract and a compound separated therefrom having excellent skin whitening effect by inhibiting the activity of the tarosinase enzyme and inhibiting melanin production, and having no safety problems. .

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising a cheonma extract having a skin whitening effect.

The cheonma extract is an extract soluble in water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably in a butanol.

The present invention also provides a cosmetic composition for skin whitening comprising a cheonma nonpolar solvent soluble extract having a skin whitening effect.

The nonpolar solvent soluble extract is an extract soluble in a nonpolar solvent, preferably ethyl acetate, selected from dichloromethane, n-hexane, chloroform and methylene chloride.

The present invention provides a cosmetic composition for skin whitening comprising the compounds isolated from the cheonma extract as an active ingredient.

Specifically, the present invention provides a cosmetic composition for skin whitening comprising 4-hydroxybenzyl alcohol (4-Hydroxybenzyl alcohol) represented by the following Chemical Formula 1 as an active ingredient.

In addition, the present invention provides a cosmetic composition for skin whitening comprising gastrodine (Gastrodin; 4-β-D-Glucopyranosyloxybenzyl alcohol) represented by Formula 2 as an active ingredient.

The extract and the compound is characterized in that it contains 0.001 to 20% by weight, preferably 0.01 to 5% by weight relative to the total weight of the composition.

In addition, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

Hereinafter, the present invention will be described in detail.

The cheonma extract of the present invention is dried in the sun and is about 1 to 20 times its weight, preferably about 2 to 10 times the volume of water, lower alcohol having 1 to 4 carbon atoms or about 1: 0.1 to 1:10 Preferably, a mixed solvent having a mixing ratio of 1: 0.2 to 1: 3, hot water for about 1 to 5 days, preferably 2 to 20 hours, at an extraction temperature of 20 to 100 ° C, preferably 50 to 95 ° C. Extraction method such as extraction, extraction to be deposited at 5 to 50 ° C. for 1 to 15 days, reflux cooling extraction, and ultrasonic extraction, preferably by extraction method by deposition 1 to 5 times, preferably 2 to 3 times After obtaining the extract by filtration under reduced pressure, the filtered and filtered extracts were mixed and concentrated under reduced pressure at 20 to 100 ℃, preferably 50 to 70 ℃ with a rotary vacuum concentrator to remove the solvent to lower water, lower alcohol having 1 to 4 carbon atoms or According to these mixed solvents Extracts of the crude extract can be obtained.

In addition, the non-polar solvent soluble extract may be suspended 1 to 5 times, preferably 2 to 4, by suspending the crude extract in water and adding about 1 to 10 times, preferably about 1 to 5 times the volume of ethyl acetate. Each fraction was separated into ethyl acetate soluble fraction and water soluble fraction, and then each solvent fraction was concentrated under reduced pressure with a rotary vacuum concentrator to remove the solvent to obtain each extract.

In addition, the polar solvent soluble extract can be obtained by adding water to the water-soluble fraction obtained through the extraction process of the non-polar solvent soluble component, the water-soluble fraction can be obtained. You can get it.

For example, according to a preferred embodiment of the present invention, the concentrated solution obtained by adding ethanol was deposited and dispersed in water, and then treated with ethyl ether to the water layer obtained by treating with n-hexane, and then ethyl acetate was added to the water layer. The water layer obtained by treatment was concentrated under reduced pressure by treating n-butanol to obtain an n-butanol layer. It is also possible to further carry out conventional fractionation processes (Harborne JB; Phytochemical methods: A guide to modern techniques of plant analysis. 3rd Ed., P 6-7, 1998).

In addition, the compound separated by the stepwise treatment of the non-polar solvent of the present invention, a mixture having a mixing ratio of chloroform: methanol mixed solvent, preferably chloroform: methanol (10: 3) to the non-polar solvent soluble extract or polar solvent soluble extract After conducting silica gel column chromatography with a solvent and repeating the silica gel column chromatography using a mixed solvent having a chloroform: methanol (9: 1) mixing ratio, more active fractions can be purified, and additionally, silica gel or Separdex column chromatography can be performed to obtain fractions in more purified form, and the purified fractions can be recrystallized or concentrated under reduced pressure to separate the compounds of the present invention in pure form.

The composition of the present invention comprises a crude extract, a non-polar solvent soluble extract or a polar solvent soluble extract obtained according to the above production method. At this time, the composition of the present invention comprises 0.01 to 10% by weight, preferably 0.1 to 5% by weight, most preferably 1 to 3% by weight based on the total weight of the composition.

The cheonma extract obtained by the extraction method and the compounds isolated therefrom exhibited excellent whitening effect because of the excellent inhibition of tyrosinase activity and melanin production by melanoma B16.

The present invention provides a cosmetic composition for skin whitening comprising the cheonma extract obtained according to the preparation method or a compound separated therefrom as an active ingredient.

The composition containing the cheonma extract or the compound of the present invention can be used in a variety of cosmetics and face wash for skin whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α tocopherol, d-α tocopherol, d-α tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α tocopherol acetate, dl-α tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleic acid ester, polyglycerin isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, cetyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-stealo Esters such as monohydroxystearic acid isostearyl; and the like.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

As animal or vegetable fats and oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Examples of nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, and POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactants, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzyl ammonium chloride, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono2-ethylhexaneglyceryl, isopropyl paramethoxy cinnamic acid, diisopropyl diisopropyl cinnamic acid ester mixture, urokane Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hydric Hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably 0.01-3% by weight is blended.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract or compound as an active ingredient, for example, such as stabilizers, solubilizers, vitamins, pigments and flavorings Phosphorus aids and carriers.

The cosmetic composition for skin whitening of the present invention may be prepared in any formulation conventionally prepared in the art, and includes, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples, Experimental Examples, and Preparation Examples.

Reference example. Equipment and solvent

Nuclear magnetic resonance analyzer used in the ¹ H-NMR and ¹³ C-NMR analysis in the present invention ¹ H-NMR is Varian (Varian, GEMINI, 200MHz) and ¹³ C-NMR is Varian (Varian, GEMINI, 50MHz) The structure was analyzed by an NMR instrument, and the solvent used was purchased from Aldrich as pyridine-d ₅ .

Example 1. Preparation of Chunma Crude Extract

1 kg of dried Cheonma (purchased at Kyungdong Market) was added to 6000 ml of 95% ethanol, and the filtrate obtained by filtration after immersion at room temperature for 7 days was concentrated using a reduced pressure concentrator (Rotavapor R-153, BUCHI). 200 g of crude extract was obtained.

Example 2. Preparation of Chunma Butanol Soluble Extract

The crude cheonma extract obtained in Example 1 was suspended in 500 g of water, and then the same amount of n-hexane was added twice. Thereafter, 500 g of ethyl ether was added twice to the water layer, and then the same amount of ethyl acetate was added twice to the resulting water layer. At this time, the resulting water layer was fractionated by treating n-butanol three times 500g three times, and then filtered and concentrated under reduced pressure to obtain 34.8g of cheonma butanol soluble extract powder.

Example 3. Isolation of Active Material from Cheonma

3-1. Separation by Silica Gel Chromatography

34.8 g of cheonma butanol soluble extract dried powder obtained in Example 2 was mixed with a mixed solvent of chloroform: methanol (10: 3) in a column filled with silica gel (Merck silica gel 60, 0.063-0.20 mm) as a developing solvent. Subsequently, the mixture was repeatedly fractionated with a mixed solvent of chloroform: methanol (9: 1) to obtain 300 fractions of 5 fractions, respectively. The fractions were concentrated under reduced pressure and recrystallized to give 2.4 g of 4-hydroxybenzyl zlcohol and 5.6 g of gastrodine (Gastrodin; 4-β-D-Glucopyranosyloxybenzyl alcohol).

3-2. Of 4-hydroxybenzyl alcohol ^One H-NMR analysis

For structural analysis and confirmation of 4-hydroxybenzyl alcohol isolated in Example 3-1, ¹ H-NMR spectrum was measured using a nuclear magnetic resonance spectrometer. Trimethylsilane was used as an internal standard and was represented as δ (ppm). The results were as follows. This is consistent with previously published data (H. Taguchi, at al., Chem. Pharm. Bull. , 29 , 55, 1981; XZ Feng at. Al., Acta Chem. Sin. , 37 , 175, 1979). Seemed.

¹ H-NMR (200 MHz, pyridine-d ₅ ), δ 9.26 (1H, s, 4-OH), 7.10 (2H, d, J = 8.04 Hz, 2,6), 6.70 (2H, d, J = 8.32 Hz, 3,5), 4.98 (1H, d, J = 5.36 Hz, 7-OH), 4.35 (2H, d, J = 5.36 Hz, 7)

3-3. Gastrodine ^One H-NMR analysis

¹ H-NMR spectra were measured using nuclear magnetic resonance spectroscopy for structural analysis and confirmation of gastrodine (Gastrodin; 4-β-D-Glucopyranosyloxybenzyl alcohol) isolated in Example 3-1. Trimethylsilane was used as an internal standard and was represented as δ (ppm). The results were as follows. Of these, a specific peak of sugar is seen around δ 3.3-4.0. Hydrogen at position 1 of the sugar was not observed due to solvent peaks and was dissolved and dissolved in DMSO- d ₆ , and sugars and other -OH groups were found at δ 4.4-5.3. This has already been published (RM Silverstein et. Al., John Wiley & Sons , Lodon, New York, 7 , p110, 1991; H. Taguchi, at al., Chem. Pharm. Bull. , 29 , p55, 1981). Showed agreement with

¹ H-NMR (200 MHz, pyridine-d ₅ ), δ 7.26 (2H, d, J = 8.56 Hz, 2,6), 7.06 (2H, d, J = 8.56 Hz, 3,5), 4.89 (1H, G1), 4.52 (2H, s, 7), 3.88 (1H, dd, J = 11.92, 1.84 Hz, G6), 3.69 (1H, dd, J = 11.92, 5.20 Hz, G6), 3.45, 3.44, 3.41, 3.40 (1H X 4, m, G3, G2, G5, G4)

Experimental Example 1. Inhibitory effect of mushroom tyrosinase activity

Using the cheonma extract prepared in Examples 1 to 3 and the substances separated therefrom as a sample to inhibit the activity of mushroom tyrosinase activity by Vanni et al. (A. Vanni, Annali di Chimica , 80 , p35, 1990) Measured. Specifically, after mixing 1.0 ml of 0.1 M potassium phosphate buffer (pH 6.8), 0.3 ml of 0.3 mg / ml L-tyrosine aqueous solution, 0.1 ml of 1,250 units / ml mushroom tyrosinase (SIGMA T-7755), the sample solution 0.2 ml of each was added to proceed the enzyme reaction at 37 ° C. for 10 minutes, and arbutin (Bioland) was used as a positive control. The absorbance of the reaction solution was measured at 480 nm to determine the effect of inhibiting tyrosinase activity by the following Equation 1, and the results are shown in Table 1 below.

Inhibition of Mushroom Tyrosinase Activity (%) = [(A-B) / A] x 100

A: Absorbance at 480 nm of reaction solution without sample

B: absorbance at 480 nm of reaction solution to which sample was added

Inhibitory effect of mushroom tyrosinase activity sample IC ₅₀ (μg / ml) Arbutin 114 Chunma Crude Extract 450 Chunma Butanol Soluble Extract (Example 2) 46 4-hydroxybenzyl alcohol > 1,000 Gastrodine > 1,000

As a result, it was confirmed that the cheonma butanol soluble extract of the present invention exhibited superior activity of mushroom tyrosinase than arbutin commonly used as a skin lightening agent.

Experimental Example 2. Inhibitory effect of tyrosinase activity

The inhibitory effect of human tyrosinase activity was measured using Vanni et al. (A. Vanni, Annali di Chimica , 80 , 35, 1990) using the cheonma extract and the active material prepared in Examples 1 to 3 as a sample.

Specifically, B16 melanoma cells (ATCC CRL6323, B16 murine melanoma cells) were inoculated in Dulbecco's modified Eagle's Medium (DMEM) medium with 10% bovine serum and 1% antibiotics at a density of 1x10 ⁵ cells and 5% for one day. Incubated at CO ₂ , 37 ° C. Then, to increase the tyrosinase production capacity of the cells, the active ingredients isolated from cheonma were changed into DMEM 10% medium treated with α-MSH (melanocyte stimulating hormone; melanocyte stimulating hormone, 0.34 ug / mL, Sigma). The wells were treated with stars (50 μg / ml and 100 μg / ml) and incubated for 3 days. Well counts according to all concentrations were prepared in triplicate. The medium was removed and the cells were removed by treatment with trypsin, and then placed in a 1.5 ml tube and centrifuged at 12,000 rpm for 5 minutes to discard the supernatant and washed twice with PBS. To the cell precipitate, 1 ml of 80% potassium phosphate buffer (pH6.8) containing 1% Triton X-100 and 2 mM PMSF (Phenylmethylsulfonyl Fluoride) was added and shaken for 20 minutes on ice. It was left for hours. Thereafter, the supernatant was recovered by centrifugation at 4 ° C. for 20 minutes at 12,000 rpm, and then 0.3 ml of the supernatant and 0.3 ml of 1.5 mM tyrosine were reacted at 37 ° C. for 3 hours. The reaction mixture was vigorously shaken and the absorbance was measured at 475 nm to determine the effect of inhibiting tyrosinase activity by the following Equation 2, and the results are shown in Table 2. Protein quantification of the supernatant was analyzed according to the Bio-rad protein quantification protocol, the absorbance of the resulting melanin was calculated by dividing the amount of protein, the value that inhibits 50% of the enzyme activity is expressed as IC ₅₀ .

% Tyrosinase activity inhibition = [(A-B) / A] x 100

A: Absorbance at 475 nm of reaction solution without sample

B: absorbance at 475 nm of the reaction solution to which the sample was added

Tyrosinase activity inhibitory effect sample IC ₅₀ (μg / ml) Arbutin 110 Chunma Crude Extract 480 Chunma Butanol Soluble Extract (Example 2) 95 4-hydroxybenzyl alcohol 50 Gastrodine > 500

As a result of measuring the inhibitory effect of tyrosinase activity in cells, it was confirmed that 4-hydroxybenzyl alcohol isolated from the soluble extract of cheonma butanol and cheonma extract of the present invention showed superior tyrosinase inhibitory activity than arbutin commonly used as a skin whitening agent. It was.

Experimental Example 3. Measurement of cell viability using B16 melanoma cells

Cell viability of B16 melanoma cells was measured using the cheonma extract and the active material prepared in Examples 1 to 3 as a sample. Specifically, B16 melanoma cells (ATCC CRL6323) were inoculated in Dulbecco's modified Eagle's Medium (DMEM) medium containing 10% bovine serum and 1% antibiotic at a density of 1 × 10 ⁵ cells, and 5% CO ₂ at 37 ° C. for one day. Incubated at. Thereafter, the mixture was changed to fresh DMEM 10% medium, and the active ingredients separated from cheonma were treated in wells by concentration and incubated for 3 days. Well counts according to all concentrations were prepared in triplicate. After 3 days, the medium was removed and treated with 1 ml of 0.33 mg / ml MTT ((3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide), Sigma M5655) for 4 hours at 37 ° C. After the reaction, MTT was removed, and then 1 ml of DMSO (dimethylsulfoxide) was added to measure the color development at 575 nm.

Cell survival rate using B16 melanoma cells Experimental substance Concentration (㎍ / mL) Cell survival rate (%) Arbutin 50 96 100 98 Chunma Crude Extract 50 97 100 101 Chunma Butanol Soluble Extract (Example 2) 50 95 100 97 4-hydroxybenzyl alcohol 50 121 100 116 Gastrodine 50 95 100 99

As a result, it was confirmed that the crude extract of the present invention and the soluble extract of cheonma butanol and 4-hydroxybenzyl alcohol and gastrodine separated therefrom showed almost equal or superior cell viability to arbutin commonly used as a skin lightening agent. See Table 3).

Experimental Example 4. Measurement of melanin biosynthesis inhibitory effect using B16 melanoma cells

Melanogenesis inhibition effect in B16 melanoma cells using the cheonma extract and the active material prepared in Examples 1 to 3 as a sample (Maeda and Fukuda, J. Soc. Cosmetic Chem. , 42 , 361, 1991).

B16 melanoma cells (ATCC CRL6323) were seeded in Dulbecco's modified Eagle's Medium (DMEM) with 10% bovine serum and 1% antibiotic at a density of 1 × 10 ⁵ cells and incubated at 5% CO ₂ , 37 ° C. for one day. Thereafter, the cells were changed to DMEM 10% medium in which α-MSH (melanocyte stimulating hormone; melanocyte stimulating hormone, 0.34 ug / mL, Sigma) was treated, and the active ingredients separated from cheonma were incubated for 3 days. . Well counts according to all concentrations were prepared in triplicate. After 3 days, the set to measure the amount of melanin collected the medium in a 1.5 mL tube and the cells were removed by trypsin treatment and the medium was transferred to the collected tube. The supernatant was discarded by centrifugation at 3,000 rpm for 30 minutes, treated with 250 μl of 1N NaOH, and then centrifuged lightly for 10 minutes in boiling water. After sonication for 10 minutes to completely dissolve the melanin absorbance was measured at 475 nm. However, the value that reduces the melanin synthesis by 50% is shown as IC ₅₀ .

Inhibitory Effects of Melanin Biosynthesis Using B16 Melanoma Cells Experimental substance IC ₅₀ (μg / ml) Arbutin 150 Chunma Crude Extract 450 Chunma Butanol Soluble Extract (Example 2) 103 4-hydroxybenzyl alcohol 45 Gastrodine 102

As a result of measuring the inhibition of melanin biosynthesis using B16 melanoma cells, 4-hydroxybenzyl alcohol and gastrodine isolated from the soluble extract of cheonma butanol and cheonma extract of the present invention are superior to melanin biosynthesis than arbutin commonly used as a skin whitening agent. It was confirmed that the inhibitory effect. In particular, unlike the relatively low tyrosinase inhibitory effects, gastrodine exhibited better melanin synthesis inhibition than arbutin, which inhibits melanin synthesis from other pathways such as TRP-1 or TRP-2, rather than direct tyrosinase activity inhibition. By this, it was estimated that the cell had a whitening effect, and the possibility of action on the mechanism related to tyrosinase gene expression, not the activity of tyrosinase, was also confirmed.

It describes a preparation example of a cosmetic composition comprising a plant mixture extract of the present invention, the present invention is not intended to limit this but only to explain in detail.

Preparation Example 1 Flexible Lotion (Skin) in Cosmetics Containing Cheonma Extract

Table 5 shows an example of the preparation of the flexible skin lotion (skin) in the cosmetic containing a sample in which the cheonma butanol soluble extract of Example 2 was dissolved in a 30% 1,3-butylene glycol aqueous solution so as to have a solid content of 1%. The comparative example is a case where the cheonma extract of the present invention is not contained.

Number Raw material Formulation Example 1 Comparative Formulation Example 1 One   30% 1,3-butylene glycol lysate of Example 2 5.0 - 2   Aloe extract 2.0 2.0 3 glycerin 3.0 3.0 4 Butylene Glycol 2.0 2.0 5 Propylene glycol 2.0 2.0 6 Polyoxyethylene (60) Cured Castor Oil 1.0 1.0 7 ethanol 10.0 10.0 8 Triethanolamine 0.1 0.1 9 antiseptic a very small amount a very small amount 10 Pigment a very small amount a very small amount 11 Spices a very small amount a very small amount 12 Purified water Remaining amount Remaining amount

At this time, the formulation example 1 was added to 3, 4, 5, and 9 times in order to dissolve while stirring. After dissolving 6 times by heating at about 60 ° C., 11 was added and stirred, followed by 12 times. Finally, 1, 2, 7, 8, and 10 were added to the dissolving agent, sufficiently stirred, and aged. In Comparative Formula 1, 3, 4, 5, and 9 were added in order to dissolve 12, followed by stirring to dissolve, and then heated to 60 ° C. for dissolution to dissolve 6, and then 11 was added to 12 for stirring. . Finally, 1, 2, 7, 8, and 10 times were added and sufficiently stirred before aging.

Preparation Example 2 Nutritional Longevity (Lotion) in Cosmetics Containing Cheonma Extract

Table 6 shows an example of the preparation of the nutrient lotion (lotion) in the cosmetic containing the sample dissolved in the cheonma butanol soluble extract of Example 2 to 30% 1,3-butylene glycol aqueous solution to 1% solids, wherein The comparative example is a case where the cheonma extract of the present invention is not contained.

Number Raw material Formulation Example 2 Comparative Formulation Example 2 One 30% 1,3-butylene glycol lysate of Example 2 10.0 - 2 Aloe extract 2.0 2.0 3 Beeswax 1.0 1.0 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.5 0.5 6 Liquid paraffin 10.0 10.0 7 Sorbitan stearate 1.0 1.0 8 Lipophilic Monostearic Acid Glycerin 0.5 0.5 9 Stearic acid 1.5 1.5 10 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 11 Propylene glycol 3.0 3.0 12 Carboxy Vinyl Polymer 0.1 0.1 13 Triethanolamine 0.2 0.2 14 antiseptic a very small amount a very small amount 15 Pigment a very small amount a very small amount 16 Spices a very small amount a very small amount 17 Purified water Remaining amount Remaining amount

In this case, Formulation Example 2 is heated to 80 ~ 85 ℃ while stirring and mixing 11, 12, 14, 17 and put into the manufacturing unit to act the emulsifier 3, 4, 5, 6, 7, 8, 9, 10 , 13 was heated to 80 to 85 ℃ to emulsify. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 16 times were added thereto, cooled to 45 ° C., 15 times were added thereto, and 1, 2 times were added to 35 ° C., cooled to 25 ° C., and aged. On the other hand, Comparative Formulation Example 2 is heated to 80 ~ 85 ℃ while stirring and mixing 11, 12, 14, 17 and put into the manufacturing unit and then actuated emulsifier 3, 4, 5, 6, 7, 8, 9, 10 , 13 was heated to 80 to 85 ℃ to emulsify. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 16 times were added thereto, cooled to 45 ° C., 15 times added thereto, cooled to 25 ° C., and aged.

Preparation Example 3 Nutritional Cream in Cosmetics Containing Cheonma Extract

Table 7 shows a preparation example of the nourishing cream in the cosmetic composition containing a sample in which the cheonma butanol soluble extract of Example 2 was dissolved in a 30% 1,3-butylene glycol aqueous solution so as to have a solid content of 1%. Is the case that does not contain the cheonma extract of the present invention.

Number Raw material Formulation Example 3 Comparative Formulation Example 3 One 30% 1,3-butylene glycol lysate of Example 2 10.0 - 2 Aloe extract 2.0 2.0 3 Beeswax 8.0 8.0 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.5 0.5 6 Liquid paraffin 20.0 20.0 7 Sorbitan stearate 1.0 1.0 8 Lipophilic Monostearate Glycerin 0.5 0.5 9 Stearic acid 1.5 1.5 10 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 11 Propylene glycol 6.0 6.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

At this time, the formulation example 3 is heated to 80 ~ 85 ℃ while stirring, mixing 11, 13, 16, and put into the manufacturing unit to act the emulsifier 3, 4, 5, 6, 7, 8, 9, 10, 12 was heated to 80 ~ 85 ℃ to emulsify. After emulsification, cool down to 50 ℃ with stirring using a stirrer, add 15 times, cool to 45 ℃, add 14 times, and cool to 35 ℃, add 1, 2, and then cool to 25 ℃. Aged. On the other hand, Comparative Formulation Example 3 is heated to 80 ~ 85 ℃ while stirring, mixing 11, 13, 16, and put into the manufacturing unit, and then actuated emulsifier 3, 4, 5, 6, 7, 8, 9, 10, 12 was heated to 80 ~ 85 ℃ to emulsify. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 15 times were added thereto, then cooled to 45 ° C., 14 times were added thereto, cooled to 25 ° C., and aged.

Preparation Example 4 Massage cream in cosmetic containing cheonma extract

Table 8 shows a preparation example of a massage cream in a cosmetic composition containing a sample in which the cheonma butanol soluble extract of Example 2 was dissolved in a 30% 1,3-butylene glycol aqueous solution to a solid content of 1%. It does not contain the cheonma extract of the present invention.

Number Raw material Formulation Example 4 Comparative Formulation Example 4 One   30% 1,3-butylene glycol lysate of Example 2 10.0 - 2   Aloe extract 2.0 2.0 3 Beeswax 10.0 10.0 4 Polysorbate 60 1.5 1.5 5 Sorbitan sesquioleate 0.8 0.8 6 Liquid paraffin 40.0 40.0 7 Squalane 5.0 5.0 8 Lipophilic Monostearic Acid Glycerin 1.0 1.0 9 Carboxy Vinyl Polymer 0.2 0.2 10 Butylene glycol 6.0 6.0 11 glycerin 6.0 6.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

At this time, the formulation example 4 is heated to 80 ~ 85 ℃ while mixing, stirring, stirring 9, 10, 11, 13, 17, and then put into the manufacturing unit to act the emulsifier 3, 4, 5, 6, 7, 8, 12 was heated to 80 ~ 85 ℃ to emulsify. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add 15 times, cool to 45 ℃, add 14 times, and cool to 35 ℃, add 1, 2, and then cool to 25 ℃. Aged. On the other hand, Comparative Formulation Example 4 is heated to 80 ~ 85 ℃ while stirring, mixing 9, 10, 11, 13, 17, and put into the manufacturing unit, and then actuated emulsifier 3, 4, 5, 6, 7, 8 , 12 was heated to 80 ~ 85 ℃ to emulsify. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 15 times were added thereto, then cooled to 45 ° C., and 14 times were added thereto, then cooled to 25 ° C. and aged.

Preparation Example 5 Nutritional Essence in Cosmetics Containing Cheonma Extract

Table 9 shows an example of preparing a nutritional essence in the cosmetic composition containing a sample in which the cheonma butanol soluble extract of Example 2 was dissolved in a 30% 1,3-butylene glycol aqueous solution so as to have a solid content of 1%. It does not contain the cheonma extract of the present invention.

Number Raw material Formulation Example 5 Comparative Formulation Example 5 One   30% 1,3-butylene glycol lysate of Example 2 10.0 - 2   Aloe extract 2.0 2.0 3 Cytostolol 13.0 13.0 4 Polyglyceryl 2-oleate 0.2 0.2 5 Ceramide 0.1 0.1 6 Ceteares-4 5.0 5.0 7 cholesterol 0.3 0.3 8 Dicetylphosphate a very small amount a very small amount 9 Concentrated glycerin a very small amount a very small amount 10 Macadamia oil a very small amount a very small amount 11 Carboxy Vinyl Polymer a very small amount a very small amount 12 Xanthan Gum a very small amount a very small amount 13 antiseptic a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Remaining amount Remaining amount

At this time, the formulation example 5 is called a nonionic amphiphilic lipid by homogenizing the raw materials 3, 4, 5, 6 and 7 at a constant temperature. The nonionic amphiphilic lipid and the raw materials 1, 2, 8, 9 and 15 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 10 is gradually added at a constant temperature to homogenize and then again micro Passed through the fluidizer again. Then, 11, 12, 13, and 14 were added to disperse to stabilize and mature. Comparative Formulation Example 5 After homogenizing the raw materials 3, 4, 5, 6, and 7 at a constant temperature to prepare nonionic amphiphilic lipids, the nonionic amphiphilic lipids and raw materials 8, 9, and 15 The mixture was homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 10 was gradually added and homogenized at a constant temperature, and then passed through the microfluidizer again. Then, 11, 12, 13, and 14 were added to disperse to stabilize and mature.

Preparation Example 6 Emulsified Foundation in Cosmetics Containing Cheonma Extract

Table 10 shows an example of the preparation of an emulsified foundation in a cosmetic composition containing a sample in which the cheonma butanol soluble extract of Example 2 was dissolved in a 30% 1,3-butylene glycol aqueous solution so as to have a solid content of 1%. Is when it does not contain the cheonma extract of the present invention.

Number Raw material Formulation Example 6 Comparative Formulation Example 6 One   30% 1,3-butylene glycol lysate of Example 2 10.0 - 2   Aloe extract 2.0 2.0 3 Beeswax 2.0 2.0 4 Pyromethicone 2.0 2.0 5 Liquid paraffin 5.0 5.0 6 Squalane 5.0 5.0 7 Stearic acid 2.0 2.0 8 Lipophilic monostearic acid glycerin 3.0 3.0 9 Macadamia oil 4.0 4.0 10 glycerin 4.0 4.0 11 Propylene glycol 3.0 3.0 12 Butylene glycol 3.0 3.0 13 Triethanolamine 1.0 1.0 14 Aluminum Magnesium Silicate 0.5 0.5 15 Pigment 12.0 12.0 16 antiseptic a very small amount a very small amount 17 Spices a very small amount a very small amount 18 Purified water Remaining amount Remaining amount

At this time, the formulation example 6 is heated to 80 ~ 85 ℃ while stirring and mixing 10, 11, 12, 13, 14, 15, 16, 18, and then put into the manufacturing unit to act the emulsifier 3, 4, 5, 6 , 7, 8, and 9 were heated to 80 to 85 ° C. and then emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 17 times were added thereto, followed by cooling to 35 ° C., 1, 2 times, and then cooled to 25 ° C. and aged. In Comparative Example 6, 10, 11, 12, 13, 14, 15, 16, and 18 were mixed and stirred, and heated to 80 to 85 ° C., put into the manufacturing unit, and then the emulsifier was activated. , 7, 8, 9 was heated to 80 ~ 85 ℃ to emulsify. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 17 times were added thereto, cooled to 25 ° C., and aged.

Clinical Trial Example 1. Whitening Effect on Human Skin Level

Twenty healthy men and women affixed an opaque tape with six holes of 1.5 cm in diameter to the upper forearm of the subject, followed by 1.5 ~ 2 times the ultraviolet (MED; Minimal Erythema Dose) of each subject. UVB) was irradiated to induce pigmentation of the skin, and two months after application of the test substances, the contrast of the skin was measured using a colorimeter. Each test substance was allowed to apply the above Preparation Examples 1 to 3 and Comparative Examples 1 to 3 twice daily over morning and evening.

The determination of the effect was determined by obtaining an L ^* value representing the contrast of the skin. In general, the L ^* value of Korean skin that does not induce artificial pigmentation is in the range of 50-70.

If the test substances are applied, the L ^* value will gradually increase. The difference in skin color (ΔL ^* ) between the start time and the completion time of application was calculated according to Equation 3 below to compare the whitening effect on human skin, and the measurement results are shown in Table 11.

ΔL ^* = L ^* value at the last day of test substance application-L ^* value before test substance application

Test substance Whitening Effect (ΔL ^* ) Formulation Example 1 4.01 Comparative Example 1 1.33 Formulation Example 2 3.98 Comparative Example 2 1.12 Execution Example 3 4.26 Comparative Example 3 0.89

As a result, it was confirmed that the skin whitening effect was excellent in the cosmetic formulations of Formulation Examples 1 to 3 prepared by adding the cheonma extract of the present invention than in Comparative Examples 1 to 3.

As described above, the cosmetic composition containing the cheonma extract of the present invention and 4-hydroxybenzyl alcohol and gastrodine isolated therefrom are tyrosinase activity and melanogenesis by melanoma B16, which are enzymes that are essential for melanin biosynthesis. Since it effectively inhibits, it can be usefully used as a cosmetic composition for preventing skin aging and whitening.

Claims (9)

A cosmetic composition for skin whitening containing Gastrodia elata BLUME extract having a skin whitening effect. The cosmetic composition of claim 1, wherein the cheonma extract is extracted with a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The cosmetic composition according to claim 2, wherein the lower alcohol is butanol. The cosmetic composition according to claim 1, wherein the cheonma extract is extracted with a nonpolar solvent selected from the group consisting of dichloromethane, n-hexane, chloroform and methylene chloride. The cosmetic composition according to claim 4, wherein the nonpolar solvent is ethyl acetate. A cosmetic composition for skin whitening comprising the compound of formula 1 isolated from the cheonma extract of claim 1 as an active ingredient. (Formula 1)

A cosmetic composition for skin whitening comprising the compound of formula 2 isolated from the cheonma extract of claim 1 as an active ingredient. (Formula 2)

The cosmetic composition according to any one of claims 1 to 7, wherein the extract or compound is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition comprises a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, Cosmetic composition, characterized in that the formulation of any one selected from the group consisting of foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.