KR101125094B1 - Cosmetic composition containing Rhapontin for protecting UV - Google Patents

Abstract

The present invention relates to a sunscreen cosmetic composition containing rapontin. More specifically, the rapontin absorbs ultraviolet rays, blocks ultraviolet rays affecting the skin, and has an effect such as anti-oxidation and anti-inflammatory, as well as almost no skin irritation. The blocking effect, of course, is characterized by helping to recover the skin damaged by ultraviolet rays.

Lapontin, UV, Cosmetics

Description

Cosmetic composition for sunscreen containing rapontin {Cosmetic composition containing Rhapontin for protecting UV}

The present invention relates to a sunscreen cosmetic composition containing rapontin. More specifically, the rapontin absorbs ultraviolet rays, blocks ultraviolet rays affecting the skin, and has an effect such as anti-oxidation and anti-inflammatory, as well as almost no skin irritation. The blocking effect, of course, is characterized by helping to recover the skin damaged by ultraviolet rays.

In general, the ultraviolet rays contained in the sunlight are divided into long wavelength ultraviolet rays (UVA, 320 to 400 nm), medium wavelength ultraviolet rays (UVB, 280 to 320 nm), and short wavelength ultraviolet rays (UVC, 200 to 280 nm) according to their wavelength ranges. Ultraviolet rays are known to cause various side effects on the skin. Ultraviolet rays not only age skin, but also cause pigmentation such as blemishes and freckles, blotch, and skin cancer. Moreover, UV rays reach 80% of the earth's surface even in shade or cloudy days, and pass through almost all glass, including automotive and house glass. In addition, in the case of clothes, synthetic fibers have little blocking effect and only 70% of natural fibers such as cotton products are blocked.

UVC in ultraviolet light is very harmful to human body, but until now it is absorbed by the ozone layer in the air and hardly reaches the surface, and UV rays affecting skin are divided into UVA and UVB. UVB is sunburn (erythema, blistering), skin cancer, pigment It promotes deposition and degeneration of skin proteins, keratinization of skin cells, UVA causes pigmentation and phototoxicity, photoallergy, penetrates into the dermis of the skin, destroys collagen and elastin, causes skin aging and causes spots and blemishes. Various kinds of sunscreens have been developed to protect the skin from ultraviolet rays harmful to the skin.

Conventional sunscreens have used organic materials such as benzophenone derivatives, paraamino benzoic acid derivatives, cinnamic acid derivatives and triazine derivatives as ultraviolet absorbers, and inorganic pigments such as titanium dioxide and zinc oxide have been used as ultraviolet scattering agents. The organic material, which is a UV absorber, may cause side effects such as allergy and irritation of the skin, troubles, and the like, and inorganic pigments may be opaque when the amount of the pigment is used in a large amount and may not be good when applied to the skin.

Therefore, in the present invention, to overcome the shortcomings of the existing sunscreen has an effective ultraviolet absorbing effect, and to examine the efficacy of separating the material that protects the skin from the ultraviolet rays from natural products.

The present inventors found that rapontin effectively absorbs ultraviolet rays, has antioxidative and anti-inflammatory effects, and is a safe substance that hardly exhibits skin irritation. The present invention has been completed by finding out that it is effective in the recovery of.

Accordingly, it is an object of the present invention to provide a cosmetic composition for sunscreen containing rapontin.

Another object of the present invention is to provide a cosmetic composition for repairing damaged skin containing rapontin.

Still another object of the present invention is to provide a cosmetic composition for anti-inflammatory prevention and treatment containing rapontin.

Still another object of the present invention is to provide an antioxidant cosmetic composition containing rapontin.

In order to solve the above problems, the present invention may include the following means.

The cosmetic composition according to the present invention is characterized in that the sunscreen containing rapontin as an active ingredient.

Cosmetic composition according to another embodiment of the present invention is for the recovery of damaged skin containing rapontin as an active ingredient.

Cosmetic composition according to another embodiment of the present invention is a cosmetic composition for anti-inflammatory prevention and treatment containing rapontin as an active ingredient.

The cosmetic composition according to another embodiment of the present invention is for antioxidant containing rapontin as an active ingredient.

The lapontin is characterized in that it is derived from sewage, the lapontin is characterized in that 0.0001 to 10% by weight relative to the total weight of the cosmetic composition.

The cosmetic composition according to the present invention contains rapontin to promote the sunscreen effect, to restore damaged skin, and to promote the effect of a functional cosmetic composition for anti-inflammatory prevention and treatment and antioxidant.

Hereinafter, the present invention will be described in detail.

Rapontin (3,3 ', 5-trihydroxy-4'-methoxystilbene 3-β-D-glucopyranoside, CAS No. 155-58-8) used in the present invention is represented by As a hydroxystilbene derivative, it is known to have weak female hormone activity.

The lapontin used in the present invention may be commercially available, and may be used for the lapontin extracted from the sewage extract.

First, the sewage extract is extracted by immersing the polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, most preferably by adding ethanol at room temperature.

The sewage is washed thoroughly with water and dried in the shade. Dry sewage is placed in an extraction container and extracted for a predetermined time at an appropriate temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The deposition temperature is 20 ~ 70 ℃, more preferably 20 ~ 40 ℃, most preferably maintained at about 30 ℃ is most effective in the prevention of decay, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After the sewage extract is obtained, solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

Prepare a mixed solution in which an organic solvent, for example, ethyl acetate is mixed in an amount of 20 to 500% (v / v) in water, add it to the dried material, stir at room temperature for 2 hours, and then stand to separate layers. In order to obtain only the crystallized product formed in the boundary layer or the organic solvent layer after layer separation, the water layer is removed and then an organic solvent is further added. The above process is repeated twice, sufficiently washed and filtered, and then the filtrate is concentrated under reduced pressure to obtain rapontin.

In the composition of the present invention, the rapontin may be contained in 0.0001 to 10% by weight based on the total weight of the composition.

Cosmetics prepared with a cosmetic composition containing the rappontine of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to the cosmetics containing the lapontin of the present invention, it can be prepared in the form of adjuvants that can be applied topically or systemically as commonly used in the field of dermatology by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to rapontin, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatology field, such as any other ingredient. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, etc. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Sewage Extract

500 g of sewage was crushed finely in a crusher, immersed in 2 L of ethanol, and warmed in a 30 ° C. water bath for at least 10 hours. The above process was repeated three times, and the obtained extract was cooled to room temperature and filtered through a filter paper to obtain a filtrate. This was concentrated under reduced pressure to produce sewage extract.

Preparation Example 2 Lapontin Purification

To 200 g of the dried material obtained in Preparation Example 1, 500 ml of water and 500 ml of ethyl acetate were added thereto, stirred at room temperature for 2 hours, and left to stand to separate layers. In order to obtain only the crystallized product formed in the boundary layer or the ethyl acetate layer after separation of the layers, the water layer was removed and then 500 ml of ethyl acetate was further added. The above process was repeated twice and sufficiently washed and filtered, and the filtrate was concentrated under reduced pressure to obtain about 15 g of lapontin.

Experimental Example 1 UV Absorption Effect

Rapontin of Preparation Example 2 was diluted to 50 ethanol to 100 ㎍ / ㎖ was measured by using a UV-Visible Spectrophotometer (Molecular Devices, USA) 50% ethanol as a control solution at a wavelength of 200 ~ 450nm, The results are shown in a graph that can confirm that the lapontin has the ultraviolet absorption ability of the present invention of FIG.

As can be seen from the experimental results, it was found that rappontin has a strong ultraviolet absorption ability in the ultraviolet wavelength range, particularly in the UVB region (280 nm to 320 nm).

Experimental Example 2 Antioxidant Activity Experiment

In order to confirm the antioxidant activity of rapontin, it was generally measured using a DPPH radical, a method of measuring antioxidant activity.

100 μg of rappontin and 0.2 mM DPPH solution, which is an antioxidant measurement solution, were mixed at 100 μl and reacted at 37 ° C. for 30 minutes, and then absorbance was measured at 520 nm using an ELISA reader.

Experiments with vitamin C (100 µg / ml), known as an antioxidant, were used to compare the relative levels of rapontin activity. As a result of the experiment, it was found that rapontin had a strong antioxidant effect as shown in Table 1.

% Antioxidant activity = (1-treated absorbance / untreated absorbance) × 100

Sample Name Vitamin c Lafontin Antioxidant activity (%) 66.2% 65.2%

Experimental Example 3 Cytoprotective Effect from UVB

In order to protect the skin cells of rappontin from being killed by UVB, 1 × 10 ⁴ human normal fibroblasts were added to each well of a 96-well microplate. Inoculate the cells to incubate for 24 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium. After incubation, the medium was removed and washed twice with PBS to remove any remaining medium. Thereafter, the rappontine prepared above was replaced with PBS adjusted to a final concentration of 200 μg / ml, and the cells were irradiated with UVB lamps (Sankyo Denki, UVB lamp 280-360 nm) for 5 minutes. After removing PBS containing rapontin, washed once with PBS to remove the remaining extracts, and incubated for 24 hours before MTT (3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium : 5 mg / ml) 10 µl of solution was added thereto, and the reaction solution was removed after standing for 4 hours. 100 μl of dimethyl sulfoxide solution was added to each well to dissolve the stained cells, and the absorbance at 570 nm was measured with a microplate reader.

By performing the above procedure, PBS was added from Equation 2 to compare the absorbance of the control group irradiated with ultraviolet rays and the absorbance when irradiated with ultraviolet rays by adding PBS containing the extract to obtain a cell protective effect of rapontin from the ultraviolet rays. The results are shown in Table 2.

Cytoprotective effect (%) = 100-{(absorbance at extract treatment-absorbance of control) / absorbance of control} X 100

Sample Name Control group Lafontin Survival rate (%) 43.6% 144.5%

According to Table 2, it was found that rapontin has an excellent effect of protecting cells from damage from UVB.

Preparation Example 3 Examples and Comparative Examples

To prepare a cosmetic containing lapontin (Example) of the above preparation, and to compare with this to prepare a cosmetic composition (comparative example) lacking the lapontin in the composition ratio of Table 3.

division Content (% by weight) ingredient Example Comparative Example 1 Lafontin 0.1 - Cetearyl Octanoate 5.0 5.0 Isopropyl Palmitate 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 Polysorbate 60 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 Spices, preservatives dose dose Purified water Balance Balance Sum 100 100

Experimental Example 4 Ultraviolet Ray Blocking Effect

     The UV blocking effect was confirmed in the human skin of Examples and Comparative Examples prepared in Table 3. Ten subjects were included in the study, and the area to be measured was coated with no coating as a control group, and the Examples and Comparative Examples were applied to each other to irradiate UVB to measure skin erythema.

Subject #One #2 # 3 #4 # 5 # 6 # 7 #8 # 9 # 10 Control group + ++ ++ + + + + + + ++ Example ± ± ± ± - + - - - ± Comparative example + ++ ++ + + + + + + +

※ Criterion ++: Very clear erythema (or erythema and blister formation) +: Clear erythema ±: Mild erythema-: No erythema

As a result of Table 4, when the application of the Example it was possible to confirm a distinct UV blocking effect compared to the control and the comparative example.

Experimental Example 5 Skin Stimulation Test

     Using the product of Examples and Comparative Examples prepared in Table 3, the skin test was performed on 20 subjects to test the skin irritation. The results are shown in Table 5. As can be seen in Table 5, almost no stimulus appeared in both the comparative example and the Example.

Number of people Example Comparative example +++ 0 0 ++ 0 0 + 0 0 ± 2 3 - 18 17

※ Criteria +++: Strong Trouble ++: Weak Trouble +: Weak Trouble ±: Normal-: Negative

Experimental Example 6 Anti-inflammatory Experiment

In order to know the anti-inflammatory effect on the Example 5 ICR mice were divided into a total of five groups experiment. Using the right ear of the ICR mouse as a test site, the ear is washed with ethanol before application of the sample, and 20 μl of the sample is continuously applied once a day for 4 days, and 1 mg of arachidonic acid is applied to the right ear 1 hour after the last application. After 1 hour, the degree of edema of the ear was repeatedly measured three times on both ears with a micrometer. The anti-inflammatory effect was determined based on the arachidonic acid treatment group as shown in Equation 3 below, and the results are shown in Table 6 below. The value is shown by the average value of each group.

Inhibition Rate (%) = (A-B) / A x 100

A: Change of control ear (thickness after sample-thickness of ear before sample treatment)

B: Change of Ear in Sample Treatment Group (Thickness of Ear after Sample Treatment-Ear Thickness Before Sample Treatment)

Ear thickness (㎛) Inhibition rate of inflammation (%) Before sample processing After sample processing Control group 255 553 - Example 251 316 78.2 Comparative example 254 548 1.3

 From the results shown in Table 6, it was found that the Example has a high anti-inflammatory effect.

Formulation Example 1 Flexible Lotion

     Formulation examples of the flexible lotion containing rapontin was prepared as shown in Table 7.

Raw material Content (% by weight) Lafontin 0.10 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

Formulation Example 2 Nutrition Cream

Formulation example of the nourishing cream containing lapontin was prepared as shown in Table 8.

Raw material Content (% by weight) Lafontin 0.2 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavoring agent Quantity Purified water Balance

1 is a graph that can be confirmed that having the ultraviolet absorption ability of the rappontine used in the present invention.

Claims (7)

Cosmetic composition for sunscreen containing rapontin as an active ingredient. delete Cosmetic composition for anti-inflammatory prevention and treatment containing lapontin as an active ingredient. An antioxidant cosmetic composition containing rapontin as an active ingredient. The cosmetic composition according to any one of claims 1, 3, and 4, wherein the rapontin is derived from sewage. The cosmetic composition according to claim 5, wherein the rapontin is 0.0001 to 10% by weight based on the total weight of the cosmetic composition. The method of claim 5, wherein the cosmetic composition, Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Essence, Nutrition Essence, Pack, Soap, Shampoo, Cleansing Foam, Cleansing Lotion Cosmetic composition, characterized in that formed in any one formulation selected from the group consisting of cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.

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