KR101124193B1 - Composition for protecting ginseng alternaria leaf blight and method for controlling ginseng alternaria leaf blight using the same - Google Patents

Abstract

The present invention ginseng spot pattern disease using a specific microbial agent and a specific fungicide ( Alternaria panax ) relates to a composition for controlling and controlling the method of mixing or alternating the same, as well as reducing the effect of the fungicide can ensure the safety of the remaining fungicides and has an excellent ginseng dot pattern disease control effect.

Ginseng spot disease, microbial agent, fungicide, mixed use, alternating

Description

Composition for protecting ginseng alternaria leaf blight and method for controlling ginseng alternaria leaf blight using the same}

The present invention provides a composition for controlling ginseng spot pattern disease and a control method using the same, and more particularly, to a composition for controlling ginseng spot pattern disease containing a specific microbial agent and a specific fungicide and a method for controlling the use or alternating spraying thereof.

Ginseng spots caused by Alternaria panax is a disease of the ground that occurs on leaves, stems, and fruits, causing huge damage to the yield of ginseng. In the suburbs of Daejeon in 2007-2008, it began to develop in the leaves in late May and until the beginning of August. However, in 2007, spotted disease began to occur in early July in Okcheon-gun, Chungbuk, and 8.4% of leaflets were reported on August 30 (Kim et al., 2007).

As mentioned above, the incidence of ginseng spot pattern disease varies depending on the region or the sea, but the length of the onset of disease causes a high number of use of pesticides. . Recently, biological control and fungicide mixtures have been used for sustainable agriculture as a method of reducing pesticides (Elad et al., 1993; Duffy, 2000; Budge and Whipps, 2001; Kondoh et al., 2001; Elmer and McGovern, 2004; Fravel et al., 2005). ). Geon et al. (2005) reported a 50% reduction in pesticides by using a combination of fungicides and vinegar solutions, and Kim et al. (1991) mixed antagonists with fungicides to control pepper blight. It has been reported to be excellent. In addition, Shim and Kim (2000) reported that the mixed treatment of antagonists and drugs was extended by about 40 days as a synergistic effect.

However, since the microbial agents used during biological control are more expensive than pesticides, it is difficult to supply farms due to an increase in operating costs by spraying microbial agents unless the frequency of spraying of pesticides is reduced.

The present inventors carried out continuous research to develop a control system for increasing the interval of spraying pesticides while maintaining a certain control effect as well as securing safety according to pesticide residues by using a microbial agent. As a result, it was confirmed that the above object can be achieved by mixing or alternately spraying a specific microbial agent and a specific fungicide, and came to complete the present invention.

Accordingly, it is an object of the present invention to provide a composition for controlling ginseng spot pattern disease comprising a specific microbial agent and a specific fungicide.

Another object of the present invention is to provide a method for controlling ginseng spot pattern bottle mixed or alternating spraying the composition for controlling the ginseng spot pattern bottle.

First, the present invention Bacillus subtilus ( Bacillus subtilis ) at least one microbial agent selected from QST713, Bacillus subtilis DBB1501; and a mixture of azoxystrobin, Boscalid, Fludioxonil and Boscalid , Copper sulfate basic, Difenoconazole, Mancozeb, Metconazole, Polyoxin B, Pyrimethanil, Triplexystrovin It relates to a composition for controlling ginseng spot pattern disease ( Alternaria panax ) comprising at least one fungicide selected from trifloxystrobin.

Second, the present invention relates to a method for controlling ginseng spot pattern bottle repeatedly sprayed on the ginseng (ginseng) 2 to 4 times at intervals of 2 to 4 weeks.

Hereinafter, the present invention will be described in detail.

Ginseng spots began to occur on May 27, 2008, and on the 9th and 23rd of June, the incidence of leaf growth gradually increased to 1.5% and 4%, respectively, to 13.35% on July 8 and 36.04% on July 22. On August 5, the rate of incidence was 100% and the incidence of spot disease increased rapidly due to continuous rainfall. Please refer to Fig.

In a preferred aspect of the present invention, the composition for controlling ginseng spot pattern disease is characterized in that the mixing ratio of the microbial agent and the fungicide is a weight ratio of 5 to 8: 5 to 2, which ensures the safety of the pesticide residue as well as the weight loss effect of the pesticide as a fungicide. It can have excellent control effect of ginseng spot pattern disease.

In a preferred embodiment of the present invention, the mixed spray is Bacillus subtilis subtilis ) QST713, Bacillus subtilis ) mixed with one or more microbial agents selected from DBB1501; and a fungicide such as azoxystrobin, triloxoxystrobin or a mixture thereof.

The mixed spraying is preferably a mixed spraying twice every two weeks by mixing Bacillus subtilis QST713 microbial agent and azoxystrobin fungicide. See B of FIG. 2.

In a preferred embodiment of the present invention, the alternating spray is azoxystrobin, boscalid, a mixture of fludioxosonyl and boscalid, coppersulfate basic, difenoconasol, mancozeb hydrate, poly for Bacillus subtilis QST713 microorganisms. One or more bactericides selected from auxin B, pyrimethanyl, and trixystrobin are mixed and sprayed alternately. The alternating spraying is preferably a mixed spray of Bacillus subtilus QST713 microbial agent and boscalid fungicide, and then mixed three times at intervals of 4 weeks by mixing the Bacillus subtilus QST713 microbial agent and azoxystrobin fungicide. See C of FIG. 2.

In addition, in the case of Bacillus subtilis DBB1501 microbial agent from azoxystrobin, boscalid, a mixture of fludioxosonyl and boscalid, cospersulfate, mancozeb hydrate, polyoxin B, pyrimethanyl, triplexoxystrobin at the time of alternating application One or more selected fungicides are mixed and sprayed alternately. Preferably, a mixed bactericidal agent is mixed with a Bacillus subtilis DBB1501 microbial agent and a fungicide which is a mixed agent of fludioxonil and boscalid, and then Bacillus subtilis DBB1501 microbial agent and triple Loxystrobin disinfectant is mixed and mixed three times at four week intervals. See D of FIG. 2.

The mixed or alternating spray of the microbial agent and the fungicide according to the present invention was found to have an excellent ginseng spot control effect of about 80% to 90%, and to reduce the fungicide of about 75 to 85%.

The present invention can ensure the safety of the pesticide residues as well as reducing the effect of pesticides by mixing and alternating spraying of a specific microbial agent and a specific fungicide and has an excellent control effect of ginseng dot pattern disease. Therefore, excellent prevention effect can be expected at the beginning of the onset, and by adjusting the number of spraying and the interval between spraying can contribute to the establishment of a ginseng spot pattern control system that maintains a constant control effect according to long onset period.

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the present invention is not limited by the following examples, and it is apparent to those skilled in the art that various modifications or changes can be made within the scope of the technical idea of the present invention.

At this time, if there is no other definition in the technical and scientific terms used, it has a meaning generally understood by those of ordinary skill in the art. Repeated descriptions of the same technical constitution and operation as those of the conventional art will be omitted.

[ Example ]

1) Characteristics of microorganisms and bactericides used for treatment

Microbicide used in this test is an eco-friendly organic materials related to the RDA registered pest control Ltd. Shinyoung Agro products in Bacillus subtilis Tiller's (Bacillus subtilis) QST713 (BS QST713 ) Bacillus as wettable powders and Co. Dongbu product sub-blocks Scotland ( Bacillus subtilis ) DBB1501 (BS DBB1501) A bactericidal agent with different granules and mechanisms was selected and used as shown in Table 1 below.

2) Control test of ginseng spot pattern disease

The ginseng spot disease control test was carried out in a random batch method 3 times in the ginseng test packaging of Chungnam National University Anseong, Muju-gun, Jeonbuk. Each microorganism or fungicide alone was first sprayed on May 27 as shown in Table 2, followed by repeated spraying at two week intervals, followed by alternating spraying of different microorganisms or fungicides and repeated spraying at two week intervals.

In addition, as shown in Table 3, the microbial agent and the fungicide are completely mixed and sprayed on the first mixed mixture on May 27, followed by repeated spraying after 4 weeks, and then different from the fungicide used in the first mixed spray on the same microbial agent used for the first mixed spray after 4 weeks. The fungicide was mixed and sprayed alternately. The microbial agent with high preventive effect was sprayed before and after onset of ginseng spot pattern disease, and the fungicide with high permeability was applied at the time of development.

3) Investigation of ginseng spot pattern disease

2) Investigation of the incidence rate of ginseng spot disease on the control test of ginseng spot disease was investigated all 20 leaves immediately before spraying the next pesticide, the final control effect was investigated 10 days after the final treatment, control ([Untreated onset rate-untreated onset rate] / untreated onset rate × 100] was calculated.

4) With microbial agents  Angle of sterilizer In treatment  Investigation of the control effect against

(One) Microbial agents  Control effect of ginseng spots on alternating sprays

The microbial agent was first treated on May 27 and sprayed twice at 14-day intervals, followed by spraying the fungicide twice and spraying the microbial agent twice again to investigate the incidence of ginseng spot pattern disease as shown in Table 4 below.

Bacterial Bacillus subtilis QST713 and Bacillus subtilus DDB1501 were sprayed twice at the beginning of the disease at 14-day intervals. . However, after 14 days of repeated application of microbial agents at 14-day intervals, the incidence of morbidity was severe in both treatment groups, and most individuals died.

On the other hand, after disinfecting boscalid, polyoxine B, two times at 14-day intervals, the morbidity rate after 14 days was 1% to 1.2%, and 4% of the untreated group was 70% to 75%. Next, mancozeb and azoxystrobin were alternately sprayed twice at 14-day intervals, and after 14 days, the incidence of morbidity was 9.00% to 10.96%, and no treatment 36.04%. Were 75.03%, Alt-5 was 73.92%, and Alt-6 was 74.81%. Finally, the incidence of 14 days after application of azoxystrobin and polyoxine B once at 14-day intervals was 10.04% to 12.09%, and slightly increased to 10.58% to 13.00% after 10 days of twice-spraying.

Li et al. (2008) reported that when the Bacillus subtilis QST713 was treated four times at 10-day intervals, the control value was 83.3%, and the control value was similar to that of the fungicide. This difference is thought to be due to the longer spacing interval and the microbial agent is thought to maintain a high control effect at intervals of 7 to 10 days. As described above, the microbial agent had an excellent preventive effect before or during the occurrence of the disease, but the treatment effect was insignificant when the disease occurred.

Therefore, the microbial agent can be used as a preventive agent for ginseng polka dots, but it was confirmed that it is inappropriate to be used as a therapeutic agent when the incidence rate increases. The use of the system was found to be an effective control method.

(2) Control effect of ginseng spot pattern disease when mixed spray with microbial agent and fungicide

In order to examine the control effect of ginseng spot pattern disease by the mixed spray after mixing the microbial agent and the fungicide, after treating three times at intervals of 27 to 28 days, the resultant leaves were shown in Table 5 below.

Bacterial Bacillus subtilus QST713, Bacillus subtilus DBB1501 and fungicide azoxystrobin, iminottadine tris albesylate polyoxine B, triple oxystrobin each mixed in different combinations and sprayed once, then 28 days The later onset leaf ratios were 0.65% to 0.72%, and 4% of the untreated group were all 80% or more. After 28 days of repeated application, the incidence rate of the leaves was 9.58% to 21.35%, and 36.04% of the untreated group.

As can be seen in B of FIG. 2, the incidence of 14 days after three repeated sprayings was 10.11% to 37.27%, and 100% of the untreated group was 89.89% for Com-1, 73.02% for Com-2, and Com-3. 87.44%, Com-4 was 84.50%, Com-5 was 62.73%, and Com-6 was 86.35%. However, after 24 days, the incidence was severe in the Com-2 and Com-5 treatment groups, and most individuals died, and the control value could not be determined. The incidence rate of the remaining treatments increased from 11.77% to 21.58% compared to 10 days ago.

(3) With microbial agents  Mixed after mixing fungicides? Control effect of ginseng spots on alternate spray

Mixed after using microbial and fungicides? In order to examine the control effect on ginseng spot pattern disease during alternating spraying, the mixed leaf spraying was carried out twice at May 27 to 28 days, and then mixed with other fungicides.

Microbial Bacillus subtilus QST713 and fungicides were mixed and sprayed once, and then 28 days later, the morbidity rate was 0.75% to 0.82%, and 4% of the untreated group was about 80%. After 28 days of repeated application, the incidence rate was 9.43% to 15.81% and untreated group 36.04%. After 24 days of alternating and disintegrating other fungicides, the incidence rate was 13.18% to 20.38%, Com-7 81.39%, Com-8 86.82%, Com-9 85.37%, Com-10 85.83 %, Com-11 82.91%, Com-12 79.62%, Com-13 86.26%, as shown in Figure 2 C, a mixture of Bacillus subtilus QST713 and metconazole and triple oxystrobin alternately sprayed All treatments except the treatment showed more than 80% control effect.

In addition, microbial Bacillus subtilis DBB1501 mixed with a disinfectant was mixed and sprayed once, and after 28 days, the morbidity rate was 0.73% to 0.86%, and 4% of the untreated group was 80%. After 28 days of repeated application, the incidence rate was 6.42% to 17.01% and untreated group 36.04%. Thereafter, the incidence of incidence after 12 days of alternate spraying after mixing other fungicides was 12.77% to 25.47%, Com-14 87.23%, Com-15 85.61%, Com-16 82.76%, Com-16 74.53%, Com-18 84.31%, Com-19 82.48%, Com-20 87.01%, as can be seen in the results of FIG. 2D, by mixing Bacillus subtilis DBB1501 with diphenoconazole and tripleoxystrobin All treatments showed more than 80% control effect except the alternative spray treatment.

(4) Comparison of pesticide weight loss when mixed or alternating microorganisms and fungicides

Ginseng spot disease control drugs are to be sprayed every 7 days. Therefore, if ginseng spot pattern disease occurs in mid-May and lasts until August, 12 to 16 pesticides should be sprayed. In this study, microbial agents and fungicides were mixed or alternately sprayed to compare pesticide spraying at intervals of 2 to 4 weeks, and the number of sprays indicated in the pesticide use method was compared with Table 7 below.

When the microbial agent and the disinfectant were sprayed alternately, it could be reduced by 50% by calculating only the 4th treatment with more than 80% control effect.

When mixing and interlocking microbial agents and fungicides, the spraying method according to the combination of microbial agents and fungicides that can control more than 80% can be used as a method for controlling ginseng spot pattern disease.

1 is a view showing the development of ginseng spot pattern disease according to the present invention,

2 is a view showing the ginseng spot pattern control effect according to the composition and control method for ginseng spot pattern control of the present invention.

Claims (9)

Bacillus subtilis DBB1501 microbial agent; and a mixture of Fludioxonil and Boscalid and a fungicide of trifloxystrobin; Alternaria panax control composition . The method of claim 1, Mixing ratio of the microbial agent and bactericide is 5 to 8: ginseng spot pattern control composition, characterized in that the weight ratio of 2 to 2. Ginseng spot pattern control method of spraying the ginseng spot pattern disease control composition according to claim 1 or 2 repeatedly applied to the ginseng (ginseng) 2 to 4 times at intervals of 2 to 4 weeks. delete delete delete delete delete The method of claim 3, The control method is a mixed spray of the Bacillus subtilis DBB1501 microbial agent and a fungicide that is a mixed agent of fludioxosonyl and boscalid, and then mixed with the Bacillus subtilus DBB1501 microbial agent and triple oxystrobin disinfectant spraying alternately Ginseng spot pattern control method.

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