KR101065605B1 - Method for soil remedy - Google Patents

Abstract

The present invention relates to a soil healing agent for the environmentally-healing of soils, particularly vinyl house soils, which cause obstacles due to series, a method for producing the same, and a soil healing method. The present invention, the first organic matter is degraded within 30 days by microorganisms in the soil; Clostridium inoculated with the first organic material; Second organic matter degraded within 90 days by microorganisms in the soil; And it provides a soil healing agent comprising a yeast inoculated in the second organic material, a method for producing the same and a soil healing method using the soil healing agent. According to the present invention, the pathogenic microorganisms are eliminated and the physicochemical properties of the soil are improved.

Description

Manufacturing method of soil remediator {Method for soil remedy}

The present invention relates to a method for producing a soil medicament, and more particularly, it is usefully used to improve the properties of soils with disorders due to grinding, particularly nematodes (microworms), micro-insects, micro-worms, pathogenicity Preparation of soil-healing agents that kill or inactivate fungi, pathogenic bacteria, and their eggs, spores, or weeds, and then restore organic soil physicochemical properties by functioning as organic fertilizer or soil modifier. It is about a method.

In general, farmers have used a large amount of chemical fertilizers and pesticides in order to increase the yield and to minimize the damage of plant pathogens, and to increase the yield of crop cultivation using vinyl houses and greenhouses for high-income crop cultivation. The physicochemical properties of various salt concentrations, pesticide residues, soil acidification, and soil breakdown have become extremely deteriorated and have reached the point where it is difficult to grow almost high quality crops. Therefore, the government has recently regulated the use of chemical fertilizers and pesticides.

In recent years, pollution-free crops grown without using pesticides have been spotlighted among consumers, and crop cultivation by natural methods or organic cultivation is being activated.

In addition, by the applicant's prior application technology, it was possible to function as a fertilizer in an environmentally friendly way using natural organic matter, to restore the healthy soil conditions and to remove existing pathogenic microorganisms.

Techniques for healing such soils are disclosed in the following documents, etc. previously filed by the applicant.

That is, in Document 1, the organic material having a soil control function to cultivate and mix microorganisms directly as part of the main material, and additionally mix organic acid granules with controlled elution time to promote decomposition in the soil and ensure the effect of soil disinfection. A fertilizer and a method for producing the same are disclosed in Document 2 below, which is a soft soil control fertilizer which is fermented in soil and controls pathogenic microorganisms in the soil and then converted into compost. And a fertilizer comprising at least 2% of yeast and Clostridium cultures.

However, in order to combat pathogenic microorganisms according to the above-described conventional techniques, there is a problem that economic burden such as preparing time-limited organic acid granules or glycosylating materials is generated.

In addition, Document 3 discloses a fermentation fertilizer manufacturing method containing a solubilized fertilizer component that can supply a comprehensive nutrient source for crop growth with high efficiency and fast efficiency without salt accumulation in soil through fermentation using chemical fertilizer. It is.

However, the following Document 3 still had a problem of using a chemical fertilizer.

[Prior Art Literature]

[Patent Documents]

(Document 1) Republic of Korea Patent Publication No. 2009-0100500 (2009.09.24 publication)

(Document 2) Republic of Korea Patent Publication No. 2008-0095219 (2008.10.28 published)

(Document 3) Republic of Korea Patent Publication No. 2007-0038825 (published April 11, 2007)

An object of the present invention is to solve the problems as described above, and the serial disturbance that can suppress the decontamination and pathogenic microorganisms through the reduction treatment of soil soil accumulation and pathogenic microorganisms due to the production of the plant culture soil intensified It is to provide a method for producing a soil healing agent.

It is another object of the present invention to provide a method for producing a soil-damaging soil healing agent for environmentally-healing the green house soil as a field soil causing obstacles due to grinding.

The present invention to achieve the above object,

(a) culturing Clostridium;

(b) inoculating the first organic material decomposed within 30 days by the microorganisms in the soil to inoculate Clostridium cultured in step (a) to obtain a first processed product;

(c) culturing the yeast;

(d) inoculating the second organic material decomposed within 90 days by the microorganisms in the soil to inoculate the yeast cultured in step (c) to obtain a second processed product; And

(e) It provides a method of producing a soil healing agent comprising the step of mixing the first workpiece and the second workpiece.

In this case, the first organic material may include at least one selected from grains or grain by-products containing starch and fruit containing sugar components, and the second organic material may include oil foil.

In addition, the Clostridium is inoculated at 0.1 to 0.2 parts by weight based on 100 parts by weight of the first organic material, incubated at 0.1 to 5.0 parts by weight to obtain the first processed material, and the yeast is 0.1 to about 100 parts by weight of the second organic material. It is preferable to inoculate 0.2 parts by weight and incubate at 0.1-5.0 parts by weight to obtain the second workpiece.

According to the present invention, the main variables that have a decisive influence on the healing and restoration of the soil are analyzed, and the nematode, fusarium, At the same time to combat a wide range of pathogenic microorganisms, such as Phytophthora (maximum), the effect is maximized, and the economic efficiency is also excellent.

In addition, according to the present invention, the soil is swelled by the generation of gas, and the organic matter and microorganisms are attached therebetween, while having the effect of improving the physical and chemical properties of the soil.

First, the concept of the present invention will be described.

From March to October in Korea, the temperature of 15cm of greenhouse depth (corresponding to -25cm in general flat) is in the range of 15 ℃ ~ 40 ℃. Hereinafter, in the present invention, the temperature in the range of 15 ° C. to 40 ° C. is defined and used as 'normal temperature'.

The present invention is based on the "environmental healing method of field soil, especially vinyl house soil, which causes obstacles due to the operation" introduced by the Korea Agricultural Promotion Administration in 2006 to improve the vulnerable parts appearing in the application process of this method. The effect after use is improved.

The method introduced by the Agricultural Promotion Agency introduced the method introduced in Japan as "soil reduction sterilization method" in Japan, and drastically reduces the pathogenic microorganisms accumulated by the series in an environmentally friendly way. In Japan, the purpose is to disinfect soil, but in Korea, it was introduced as a way to heal salt accumulation phenomenon and improve soil physicochemical properties in addition to soil disinfection.

The method introduced by the Agricultural Promotion Agency mixes bran with soil and covers the surface with vinyl to allow anaerobic fermentation to proceed in the soil for the first 20 days, and then peels off the vinyl and tills the soil for 10 days. The resulting organic acid is volatilized and converted to organic fertilizer or compost.

Although this method has not been introduced in Korea for a long time, it is evaluated by many farmers as being relatively economical, easy to use and effective. However, after years of application, problems and vulnerabilities have been found and need to be improved to improve the utility of farmers.

Using the organic material as described above, the soil reduction treatment results according to the present invention are shown in Table 1.

In the present invention, the main variables that have a decisive influence on the healing and restoration of the soil in an environmentally friendly manner are analyzed, and the preferred types of materials, their processing methods, and their compositions are determined based on the mechanisms in which these variables work over time.

The material used in the present invention is an environmentally friendly natural organic material, and when processed and used according to the present invention, it simultaneously eliminates a wide variety of pathogenic microorganisms such as nematodes, fusarium, and phytophthora, and thus its efficacy Is much more important and economically superior. In addition, the softening soil healing agent according to the present invention greatly reduces the occurrence of the pathogenic microorganisms do not die due to insufficient fermentation of the microorganisms in the method introduced by the Agriculture Promotion Agency.

Conventionally, chemical fertilizers mixed with organic substances are expected to reduce the loss of chemical fertilizer components to the external soil by the absorption of microorganisms, but do not have a specific plan for which microorganisms should be used until. Thus, if the pathogenic microorganisms absorb chemical fertilizers, they may further damage crops. In contrast, the present invention specifically considers which material components are necessary for the activity of any microorganism at what time. Therefore, the chemical fertilizer mixed with the organic material can not be designed to have the organic composition as the present invention.

The present invention is based on the following matters, and since the variables defined in the present application have a decisive influence on the healing and restoration performance of the soil, it is a standard for selecting an optimal material and determining a composition corresponding to each variable.

First, the inventors have discovered that the main variable acting in the first phase of the various stages of soil healing progress is the density of aerobic microorganisms. The density of aerobic microorganisms plays a decisive role in the composition of the anaerobic environment.

The composition of PLFA (phospholipid fatty acid: phospholipid fatty acid) was as shown in Table 2 and Table 3 (Chloroform. GC. Μmol / g.soil).

35 5 days 10 days 15th 20 days 30 days No treatment 8,316 7,102 7,997 7,781 7,206 bran 28,035 22,163 41,182 39,987 20,477

12 0 5 days 10 days No treatment 5,471 - - bran 18,697 15,961 molasses 18,697 12,887

PLFA is measured by extracting only biophospholipids of microorganisms, and it is possible to estimate the total density of microorganisms. In Tables 2 and 3, the untreated is left as it is without covering the vinyl.

In the short term, aerobic microorganisms were found to function optimally when aerobic microbial growth reached the maximum density in the soil. When the temperature is low, the density of microorganisms in the soil has changed slowly at the level lower than the maximum value of normal temperature (for example, the temperature of 35 cm at 15 cm of soil depth), and organic matter in the soil after 30 days of treatment. The amount was relatively large, indicating that the decomposition was not sufficient.

In addition, the inventors collected air between the surface and the vinyl covered thereon at 2, 5, 11, and 20 days intervals and analyzed with Checkmate II. The analysis results are shown in Table 4.

Date 0 2 days 5 days 11th 20 days No treatment 20 19 17 16 19 bran 20 0.1 0 1.8 0 Cryogenic bran 20 11.3 8.8 1.0 0.1 Low temperature molasses 20 2 0 0 0

The difference between the room temperature and the low temperature of bran is different from the graph of oxygen concentration at low temperature. The decrease in oxygen is due to the respiration of microorganisms. When oxygen is below 1.0%, it is called anaerobic. In this case, the microbes that breathe oxygen should die in theory. Seeds do not die.

The concentration of organic matter after 30 days is shown in Tables 5 and 6 (Tyurin method. 0-30 cm soil. G / kg.soil0).

35 0 days 20 days 30 days No treatment 23.6 23.0 22.6 bran 28.2 27.2 25.3

12 0 days 20 days 30 days No treatment 15 - - bran 19.6 19 18.2 molasses 19.7 18.7 17.3

The experiment of the above [Table 6] was conducted by maintaining the temperature in the soil at 12 ° C. with the buried geothermal wire when the outside air temperature was 0 ° C. On the 13th day, after weeding of bran and molasses, 30 I could keep growing until work.

The method introduced by the Agricultural Promotion Administration fails to process if the fermentation period is long when the soil temperature is low or water is well drained.

It can be seen that it is very important that the aerobic microorganisms reach the maximum density in the soil in a short period of time.

Secondly, the inventors found that the main variable that acts in the second stage over time, as the soil healing progresses, is the amount of enzyme secreted in the first stage. The amount of this enzyme causes structural damage to the seeds of microbial spores and weeds.

If the wheat is treated with soil in the soil at normal temperature according to the method introduced by the National Agricultural Agency, the density of the microorganisms in the soil reaches the maximum after 5 days of treatment, thereby creating a sufficient anaerobic environment, and representative major soil enzymes. Their concentration was also observed to increase several times as high as the levels present in the soil before treatment.

These enzymes are as follows.

α-amylase (1 unit = 1mg maltose / g.soil / min)

β-amylase (1 unit = 1mg maltose / g.soil / min)

protease (1 unit = 1 μg tyrosine / g.soil / min)

dehydrogenase (1 unit = 1 μmol triphnylformazane / g.soil / hr)

phosphomonoesterase (1 unit = 1 μmol PNP / g.soil / min)

Amylase is quantitatively large among these enzymes, and other enzymes are significantly smaller in specific gravity.

In addition, the rate of change of the enzyme is shown in Tables 7-9. Table 7 is the amylase unit, Table 8 is the protease unit, and Table 9 is the dehydrogenase unit.

12 0 days 5 days 10 days No treatment 0.0 0.0 0.0 bran - 3.1 2.8 molasses - 0.9 0.8

12 0 days 5 days 10 days No treatment 6.4 6.7 6.4 bran - 41.9 43.4 molasses - 23.2 7.1

12 0 days 5 days 10 days No treatment 2.1 2.4 2.3 bran - 20.0 14.1 molasses - 30.0 25.3

The amount of bran introduced by the above-mentioned method is less than 20% of the amount of existing organic matter present in the soil, so the amount of enzyme secreted in relation to bran is related to organic matter in the soil of the same weight. It is estimated to be more than one hundred times the amount of enzyme secreted. The amount of such enzymes is almost impossible to perform economically for artificial administration.

Treatment of wheat bran with soil in the manner introduced by the Agency promotes dead nematodes at 5 days after treatment, and increases the number of seeds and microbial spores of dead plants around 10 days after treatment. Observed in a broken state. Spores and seeds have a strong outer wall and are not easily damaged. Also at this time the density of anaerobic microorganisms is not high and the amount of organic acids produced by them is not sufficient.

Thus, during this period, soil enzymes play an important role in damaging the structure of seeds and spores by directly lysing the cell wall or stimulating germination to induce the cell wall to weaken itself.

Third, the inventors found that the main variable acting in the third stage of the time stage of the soil healing progress is the density of anaerobic microbes in the soil.

5 days after the 35 ° C part of the PLFA data of Table 2 and Table 3 can be seen as anaerobic microorganisms because there is no air. This is why the Ministry of Agriculture has emphasized organic acids in its treatment because organic acids are a byproduct of anaerobic microorganisms and are directly related to the density of microorganisms.

In addition, if the bran is treated in the soil according to the method introduced by the National Agricultural Agency, the anaerobic microorganisms enter the logarithmic growth stage about 12 days after the treatment, and the density in the soil reaches the maximum point about 15 days after the treatment. Concentrations also spike in the same curve and reach maximum. And at this time the seeds of weeds and the spores of microbes die in the soil.

Fourthly, the inventors have found that disinfection of soil is not caused by any particular substance or type of microorganism, but only when all three of these variables are operated at the right time.

Fifth, the present inventors slowly decompose organic matter and change the density of microorganism at low temperature when the temperature is lower than 15 ℃, and artificially increase the initial density of microorganism by 10 times and input enzyme at 10 ℃. It was found that the degradation activity of the microorganisms is normal, and the maximum density of the microorganisms is also increased to a level similar to that at normal temperature. And especially anaerobic microorganisms were found to be more affected by low temperatures.

This means that anaerobic microorganisms consume only 1/8 of the amount of energy obtained by decomposing organic matter, and thus, it takes a relatively long time to produce enzymes. It is believed that this is the same reason as previous reports that organic matter can be broken down.

Therefore, the present application increases the initial density of anaerobic microorganisms, and proposes a method for replenishing enzymes.

In the present application, it was found that it is more preferable to proliferate enzymes and microorganisms by primary fermentation than to use an appropriate organic substance as it is, based on the above-discovered knowledge.

Organic components that achieve sufficient anaerobic conditions by reaching the maximum density of microorganisms in the initial short term are water-soluble low molecular organics that are readily absorbed or readily decomposed and absorbed by the microorganisms.

In addition, the organic component that attracts a large amount of enzyme secretion in a short time is a high molecular weight and hydrophilic polymer organic material than the water-soluble low molecular organic material.

In the case of treatment using only molasses or starch, soil disinfection fails, unlike treatment with only bran, so that both water-soluble low-molecular organic materials and hydrophilic high-molecular substances have to work at different times to obtain desirable soil disinfection results. I checked my luggage.

On the other hand, yeast, yeast granules, clostridium granules, and naturally dried raw sake gourd were added to obtain a desirable effect. In particular, it was found that the addition of both raw sake gourd, yeast granules and clostridial granules was most preferable.

Therefore, the composition of the organic material is immediately consumed by the microorganisms and is consumed up to 30 days, and the ingredients consumed within 30 days after 90 days, and this is fermented to some extent with microorganisms in yeast and Clostridium. It is preferable to make it.

Hereinafter, the present invention will be described in detail based on the technical spirit of the present invention as described above.

Soil healing agent prepared according to the present invention, by removing the pathogenic microorganisms in the soil (field) by using the activity of the microorganisms contained in the soil, to restore a variety of microorganisms favorable to crop growth, and then to the environmentally friendly It is a soil healing agent. Composition content (parts by weight) in the present invention is based on dry weight.

Soil healing agent prepared according to the present invention when applied to the soil without covering the vinyl acts like a general organic fertilizer, so that the soil control effect is very minimal 70% or more of the pathogenic microorganisms can survive, spores or eggs Most may not die.

However, when the surface is covered with vinyl, the soil control effect is excellent, which is not comparable with general organic fertilizer. Pathogenic microorganisms incorporated in soil remediation agents are also controlled. Therefore, the soil healing agent according to the present invention is preferably used to cover the vinyl after application (injection) to the soil.

Control of the green house soil must be completed at the time of crop change, so kill nematodes and their sheaths, weed seeds and other pathogenic microorganisms within 20 days. Therefore, some of the organic materials are pre-fermented to increase the microorganisms of yeast and Clostridium and various enzymes. Although there are many kinds of microorganisms found in soils in which soil control is advanced, the present invention applies yeast and clostridial belonging to important microorganisms causing bottlenecks.

Hereinafter, the method for producing a soil healing agent according to the present invention will be described in more detail.

The method for preparing a soil healing agent according to the present invention includes the following steps (a) to (e).

(a) preparing Clostridium;

(b) culturing the Clostridium of step (a) to a first organic material decomposed within 30 days by microorganisms in the soil to obtain a first processed product;

(c) preparing yeast;

(d) culturing the yeast of step (c) to a second organic material decomposed within 90 days by microorganisms in soil to obtain a second processed product;

(e) mixing the first workpiece and the second workpiece:

First, Clostridium is cultured.

Clostridium has a relatively slow growth rate and requires a culture technology that suppresses growth of Bacillus or lactic acid bacteria, and can be achieved by using the composition of the material, the reaction temperature, the moisture content of the material, the reaction time, and the amount of oxygen supplied.

Clostridium is present in a large number of soy sauce brewing residues and Sikhye fermentation residues, and can be subcultured by itself. The native Clostridium can be cultured by itself, and can be collected and used as a layer in the upper layer of soy sauce or doenjang and methane fermenter. In order to suppress the Clostridium genome, which degrades proteins, repeated passages with pH of 3.0-6.0 can be used to create a seed. However, it is not necessary to purely separate the original species since the coexistence of complex species rather than a single species is preferred because of the higher degradability. Chlostridium acetobutyricum, C. butyricum, C. cellulovorans.

In addition, since Clostridium is commercially available in powder form as a medicine, its use is also possible.

Next, the first organic material is prepared. The first organic material is selected from natural organic material that is degraded within 30 days at normal temperature (range from 15 ° C. to 40 ° C.) by microorganisms in the soil.

In the present invention, the first organic material is not limited as long as it is a natural organic material decomposed within 30 days by microorganisms in the soil. Preferably, the first organic substance includes at least one selected from cereals or cereal by-products rich in starch, and fruits rich in sugar components. At this time, the grains may be one or more selected from wheat, rice, potatoes and sweet potatoes. In addition, the first organic material may further include one or more selected from molasses, sugar, glucose, fructose, maltose, honey, syrup and fruit juice in one or more selected from cereals and fruits. In addition, the said 1st organic substance may be the mixed goods and processed goods containing 30 weight% or more of the above-mentioned organic substance materials.

The first organic material is inoculated and incubated with Clostridium to obtain a first processed product. At this time, Clostridium forms spores. If the cultivation proceeds a lot, the organic material used for fermentation in the soil is consumed in advance, so that the culture may be carried out at 1.0 to 5.0 parts by weight (dry weight ratio) based on 100 parts by weight of the first organic material added to promote fermentation in the soil. do. After that, grind to the size of the rice grains again.

At this time, the first organic material is partially decomposed and the state of the first workpiece is mixed with the various enzymes produced, and the cultured Clostridium microorganisms contained in the first workpiece is in the resting state and activated when applied to the soil It functions as a chain reaction catalyst to decompose organic matter and dead bodies in its own components and soil. Clostridium may be inoculated at 0.1 to 0.2 parts by weight with respect to 100 parts by weight of the first organic material, and cultured at 0.1 to 5.0 parts by weight to obtain a first processed product.

The yeast is cultured separately from the above.

Fungi and yeasts start to decrease from 5 days after the increase of the density of about 1,000 times during the soil control according to the present invention to zero.

Yeast is slow to grow, so it is important to artificially increase the initial density to ensure effectiveness in a given period of time. Therefore, in the present invention, the second organic material is inoculated with yeast to increase the number of yeast population and to increase the amount of enzyme. At this time, a culture technique for inhibiting growth of Bacillus and the like that proliferates faster than the yeast is required, and this may be achieved by using the composition of the material, the reaction temperature, the moisture content of the material, the reaction time, and the supply amount of oxygen.

Since it is preferable to produce various enzymes, the yeast to be applied to the present invention does not need to be pure yeast of a specific species, and it is possible to carry out subculture by itself, such as soil yeast, brewing yeast, alcoholic yeast, and the like.

In cultivation of yeast, the control of nutrient concentration, temperature control and oxygen control are very important. Heat of reaction occurs, and if the sugar concentration in the medium is high, alcohol fermentation is performed. If sugar is insufficient, autodigestion occurs and decomposes. Oxygen also affects this. Yeast is available on the market in powder form.

Prepare a second organic matter. The second organic material is selected from natural organic material which is decomposed within 90 days at normal temperature (15 ° C. to 40 ° C.) by microorganisms in the soil. More specifically, it is selected from natural organic material which decomposes within 30 to 90 days.

In the present invention, the second organic material is not limited so long as it is a natural organic material decomposed within 90 days by microorganisms in the soil, more specifically, a natural organic material decomposed within 30 to 90 days. The second organic substance preferably contains oil foil. The oil foil is left after squeezing oil, and may be selected, for example, from oil foil obtained from sesame seeds, sesame seeds and perilla. In addition, the second organic material may further include one or more selected from tapioca and various starches, which are edible starches collected from the root of cassava, which is a tropical crop. In addition, the said 2nd organic substance may be the mixed goods and processed goods containing 30 weight% or more of the above-mentioned organic substance materials.

The second organic material is inoculated and incubated with the yeast to obtain a second processed product. At this time, let the yeast form spores. If the cultivation proceeds a lot, the organic material used for fermentation in the soil is consumed in advance, so that the culture is carried out at 1.0 to 5.0 parts by weight (dry weight ratio) based on 100 parts by weight of the second organic material added to promote fermentation in the soil. . After that, grind to the size of the rice grains again.

At this time, the second organic material is partially decomposed and is in a state of a second processed material in which various enzymes are mixed, and microorganisms such as cultured yeasts contained in the second processed material remain in a resting state and are activated when applied to soil. By producing enzymes and feeding the degraded nutrients to the soil microorganisms, the aerobic microorganisms increase rapidly, resulting in the consumption of oxygen in the soil and the physicochemical properties of the soil. Yeast may be inoculated at 0.1 to 0.2 parts by weight based on 100 parts by weight of the second organic material, and cultured at 0.1 to 5.0 parts by weight to obtain the second processed product.

Next, the first workpiece and the second workpiece are mixed to form a mixed organic material (soil healing agent). At this time, the first workpiece is 20 to 80 parts by weight, the second workpiece is preferably mixed in the range of 20 to 80 parts by weight based on 100 parts by weight of the soil healing agent, and easily mixed by using a conventional mixer. Can be. Preferably, the first workpiece may be mixed in the range of 40 to 70 parts by weight, and the second workpiece may be mixed in the range of 30 to 60 parts by weight, and most preferably, the first and second workpieces may have a weight ratio of 55 to 65:35 to 45. It is good to mix with.

In addition, the method for producing a soil healing agent according to the present invention may further comprise the step of further mixing the compost to the mixture of step (e) the first workpiece and the second workpiece is mixed. In addition, in addition to the compost can be mixed with other substances. For example, substances such as crop growth promoters, immune enhancers, and nutritional supplements may be further mixed.

In the above, the soil healing agent including the first workpiece and the second workpiece has been described, but the present invention is not limited thereto. That is, according to another aspect of the present invention, in addition to the first workpiece and the second workpiece, the first organic material and / or the second organic material in which Clostridium and yeast are not cultured may be further mixed and used.

The soil healing agent according to the present invention improves the physicochemical properties of the soil while eradicating pathogenic microorganisms and at the same time swelling the soil by the generation of gas and the effect of pasting between them. Soil healing agents according to the present invention can be usefully applied to the healing of soft soil. In particular, it can be used to environmentally cure field soils that cause obstacles due to serialization. For example, it can be useful for planting crops by tilling and tilling the soil moisture content to be above the maximum water capacity after mixing with tillage soils, especially in a plastic house, which cause obstacles due to grinding. .

Claims (5)

(a) preparing Clostridium;
(b) 100 parts by weight of the first organic material comprising at least one selected from grains or grain by-products containing starch and sugar-containing fruits that are degraded within 30 days by microorganisms in the soil; Culturing the Clostridium at 1.0 to 5.0 parts by weight to obtain a first workpiece;
(c) preparing yeast;
(d) culturing the yeast of step (c) at 1.0 to 5.0 parts by weight based on 100 parts by weight of the second organic material decomposed within 90 days by microorganisms in the soil to obtain a second processed product; And
(e) manufacturing a soil healing agent comprising the step of mixing the first workpiece and the second workpiece.
delete The method of claim 1,
The second organic material is a method of producing a soil healing agent, characterized in that it comprises oil (박).
The method of claim 1,
The method of claim 1, further comprising the step of mixing the compost in addition to the mixture obtained in step (e).
delete