KR100970569B1 - Candida pelliculosa sw001(kfcc11481p) and silage additive, odor control agent, fermenting agent having the same - Google Patents

Abstract

PURPOSE: A novel Candida pelliculosa SW001(KFCC11481P) is provided to ensure strong fermentation ability even at low temperature and with less moisture. CONSTITUTION: A Candida pelliculosa SW001(KFCC11481P) is able to proliferate under 40% of moisture content and rapidly proliferate at low temperature. The Candida pelliculosa also ferments under severe condition at 0.1% of carbon source of glucose or sucrose. A microorganism agent for livestock feed contains the Candida pelliculosa SW001. A silage additive contains Candida pelliculosa SW001.

Description

Candida Felicurosa SZ001 (WFCC11481P), silage additive, manure malodor improving agent and agricultural product fermentation agent {Candida pelliculosa SW001 (KFCC11481P) AND SILAGE ADDITIVE, ODOR CONTROL AGENT, FERMENTING AGENT HAVING THE SAME}

The present invention has a strong fermentation at low temperatures and low moisture, Candida pelliculosa SW001 (KFCC11481P) and silage additives using the same to improve the palatability of the livestock by the fermentation odor generated by alcoholic fermentation during fermentation, It relates to a manure malodor improving agent and agricultural product fermentation agent.

Currently, feed additives or environmental improvers using microorganisms mainly use beneficial bacteria such as yeasts, fungi, lactobacillus, actinomycetes, and photosynthetic bacteria to increase the efficiency of livestock feed or increase immunity to reduce mortality and reduce odors. , Compost maturity promotion.

As a feed additive using microorganisms, Korean Patent No. 100382, Patent No. 134562, Patent No. 165955 have been proposed.

Common bacteria such as yeast, mold, and lactic acid bacteria used in the above invention are multiplying at a water content of 40% or more, and slowing down the growth rate, and fermentation is impossible unless fermentation is possible or inhibited in the nutrient-rich medium. There is an impossible problem.

The present invention for solving such a conventional problem is possible to proliferate even in water content less than 40%, it is possible to proliferate even at low temperatures, fast fermentation time and fermentation is possible even when other bacteria predominate as well as glucose or sucrose It is an object of the present invention to provide new strains, silage additives, manure odor improvement agents and agricultural fermentation agents that can ferment even in extreme nutritional conditions with less than 0.1% carbon source.

The present invention for achieving the above object provides Candida pelliculosa SW001 (KFCC11481P).

And Candida pelliculosa (Candida pelliculosa) SW001 (KFCC11481P) provides a livestock feed microbial agent characterized in that it comprises.

In addition, it provides a silage additive characterized in that it comprises Candida pelliculosa SW001 (KFCC11481P).

It provides a manure odor improvement agent comprising Candida pelliculosa SW001 (KFCC11481P).

In addition, Candida pelliculosa (Candida pelliculosa) SW001 (KFCC11481P) provides a agricultural product fermentation agent comprising a.

Hereinafter, the present invention will be described in more detail.

The present invention is capable of proliferation even at a moisture content of less than 40%, possible to proliferate even at low temperatures, fast fermentation time, and fermentation is possible even when other bacteria are predominant, as well as extreme nutrients such as glucose or sucrose and less than 0.1% carbon source. It is related to the new strain Candida pelliculosa SW001 (KFCC11481P) which can be fermented even in a state and can be fermented even in a low protein state. The new strain Candida pelliculosa SW001 (KFCC11481P) of the present invention was deposited with the microbial accession number KFCC11481P to the Korea Microorganism Conservation Center on February 24, 2010.

Microbiological characteristics of the new strain Candida pelliculosa SW001 (KFCC11481P) of the present invention are as follows.

1) Morphology

When incubated for 2 days at 30 ℃ in YM agar plate

Fungal form: white or cream color colony formation

Mycelium: Yes

Mycelial size: 3-5μ

Cell Form: Cylindrical

Mobility: None

2) physiological properties

Possible growth temperature: 10 ~ 45 ℃

Optimum growth temperature: 25 ~ 35 ℃

Growth possible pH: 3.0 ~ 8.5

Optimum pH: 5.0 ~ 6.0

Effect on Oxygen: Breathable Anaerobic

3) sugar fermentation test

Glucose: +

Glycerol: +

D-Gluconate:-

Arabinose:-

Xylose: +

Adonitol:-

Xylitol:-

Galactose: +

Inositol:-

Sorbitol:-

Alpha Methyl-Di-Glucoside: +

Enacetyl Glucosamine:-

Cellobiose: +

Lactose:-

Maltose: +

Sucrose: +

Trehalose: +

Melegitos: +

Rafinos: +

('+' Above indicates use or reaction, '-' indicates unused or unresponsive)

Candida pelliculosa SW001 (KFCC11481P) is a type of yeast that not only acts as a source of protein, but also promotes the metabolism of livestock because it is present in yeast in a form that is easy to absorb important metal ions in livestock. And, it contains a flavor component such as nucleic acid, glutamic acid, amino acid supply, UGF, alcohol and acts to enhance the palatability of the livestock.

The new strain Candida pelliculosa SW001 (KFCC11481P) can be used for various purposes as a silage additive, manure odor improving agent, and agricultural fermentation agent.

Candida pelliculosa SW001 (KFCC11481P) of the present invention is capable of proliferation even at a moisture content of less than 40%, can be grown at low temperatures, fermentation time is fast and fermentation is possible even when other bacteria are predominant. It is possible to ferment even in extreme nutrient condition with less than 0.1% carbon source such as glucose and sucrose, and it is possible to ferment even in the state of low protein.

Silage additives, including candida pelliculosa SW001 (KFCC11481P), manure odor enhancers and agricultural fermentation agents, improve palatability, digestive capacity, meat quality, meat grade, nutrient increase, fermentation, immune boosting effect, livestock environment Improvement, antibiotic replacement effect and odor, flies prevention effect, barn environment improvement, growth promotion effect.

Such Candida pelliculosa SW001 (KFCC11481P) is cultured and used alone or adsorbed to grain processing by-products such as skim, soybean meal, rice bran, etc. together with yeast, actinomycetes, photosynthetic bacteria, and fungi. If you can maximize the effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. The scope of the present invention is not limited to the following Examples.

Example 1

Candida pelliculosa (Candida pelliculosa) SW001 (KFCC11481P) was cultured in 10g of glucose, 5g of peptone, 3g of yeast extract, 3g of malt extract in 1L of water and sterilized at 121 ℃ for 15 minutes. The sterilized medium was cooled to 30 ° C., and then inoculated with about 1% of the seed culture solution previously incubated at 30 ° C. for 24 hours in the same medium. The number of bacteria of Candida pelliculosa SW001 (KFCC11481P) obtained at this time was 10 ⁸ cfu (colony forming unit) per ml ml.

After cultivating Candida pelliculosa SW001 (KFCC11481P) bacteria, the liquid culture solution was mixed with grain processing by-products such as skim, rice bran, soybean meal, wheat bran, bran, and chaff at a weight ratio of 0.5: 1. Candida pelliculosa SW001 (KFCC11481P) culture was prepared by drying until the moisture content is less than 10% in a ~ 50 ℃ dryer.

Experimental Example 1 Inhibition Effect of Fly Larvae

Candida pelliculosa SW001 (KFCC11481P) culture of Example 1 was added 0.1% to the pig flour, and the effect of inhibiting the fermentation state, the smell, and the occurrence of fly larvae was measured in comparison with the pig flour not added. Each experimental group was left for 24 hours in a large number of flies, recovered and incubated for 8 days at 35 ℃ to confirm the development of fly larvae, the results are shown in Table 1.

division 4 days 6 days past 8 days past Remarks  No addition △ O O
X: Does not occur
△: slightly occurs
O: It happens a lot 0.1% addition X △ △ 0.5% addition X X △ 1% addition X X X 5% addition X X X

Candida pelliculosa SW001 (KFCC11481P) culture 0.1% of Example 1, as shown in Table 1. When 0.5%, 1%, and 5% were added, the effect of suppressing the occurrence of fly larvae appeared, and it was confirmed that almost no fly larvae occurred even after a lapse of time.

Experimental Example 2 Inhibition Effect of Ammonia Gas

The Candida pelliculosa SW001 (KFCC11481P) culture of Example 1 was added 0.1% to pig meal and poultry, and then the ammonia gas inhibitory effect was measured in comparison with the added pig meal and poultry.

Each experimental group was measured for ammonia gas concentration using a detection tube after 10 days, 30 days, 90 days at 35 ℃, the results are shown in Table 2.

division 10 days past 30 days elapsed 90 days elapsed Stepwise No addition 35 ppm 52 ppm 27 ppm 0.1% added 5 ppm 2 ppm 2 ppm Money No addition 50 ppm 70 ppm 35 ppm 0.1% added 7 ppm 3 ppm 1 ppm

As shown in Table 2, when 0.1% of Candida pelliculosa SW001 (KFCC11481P) culture was added, it was confirmed that the ammonia concentration was significantly reduced in both pig meal and chicken meal.

Experimental Example 3 Scattering water such as a laying hen, weight of eggs, ammonia gas generation amount, etc.

200 laying eggs and 12,000 broilers were divided into two groups, and one group was treated with the candida pelliculosa SW001 (KFCC11481P) culture of Example 1 in the feed, and the other group was conventionally used. After feeding only feed, the number of laying eggs, egg weight, ammonia gas generation, fly generation, growth status, etc. were compared. And the results for laying hens (Roman system) are shown in Table 3, the results for broilers (Abaenicker) is shown in Table 4.

Item No furniture Candida Felicosa Addition        Remarks Total scattering   24,408        28,116
X: Does not occur
△: slightly occurs
O: It happens a lot Ran weight (Kg / June)   1,487        1,729 Average spawning number / June   136        156 Average weight (g / piece)   60.9        61.5 Ammonia gas   13 ppm        5 ppm Fly outbreak     O         △

division No furniture Candida Felicosa Addition       Remarks Feeding water (mari) 12,000 12,000


X: Does not occur
△: slightly occurs
O: It happens a lot Shipment (mari) 11,184 11,531 % Shipment 93.2% 96.1% Day of Shipment (Days) 35 35 Average Shipping Weight (Kg) 1.66 1.71 Feed intake (ton) 33.6 34.5 Feed retention rate (%) 1.81 1.75 Ammonia gas 28 ppm 6 ppm Fly outbreak O △

As shown in Table 3 and Table 4, the production rate of the candida pelliculosa SW001 (KFCC11481P) culture group was confirmed as a result of checking the number of laying eggs, egg weight, ammonia gas generation, fly generation amount, growth status, etc. The broiler, broiler and its environment were very good. As a result, the reduction of odor and the occurrence of flies were remarkably reduced, and it was confirmed that the growth promotion and productivity were improved by improving the livestock environment as well as livestock.

Experimental Example 4 Growth Conditions and Breeding Environment of Piglets

50 piglets and 50 pigs were divided into two groups, and one group was fed the Candida pelliculosa SW001 (KFCC11481P) culture of Example 1 to the feed by 0.1%, and the other group was Only feeds were administered and compared. The results for the piglets are shown in Table 5, and the results for the growing pigs are shown in Table 6.

Item No furniture Candida Felicosa Addition Remarks Average Starting Weight (Kg) 14.80 13.99

X: Does not occur
△: slightly occurs
O: It happens a lot Average end weight (Kg) 31.80 32.14 Weight increase (Kg) 16.99 18.15 Weight gain (Kg / day) 0.56 0.60 Average feed intake (Kg) 34.94 35.30 Feed efficiency
(Weight gain / feed intake) 0.48 0.51 Fly outbreak O △

Item No furniture Candida Felicosa Addition Remarks Disclosure head (mari) 50 50


X: Does not occur
△: slightly occurs
O: It happens a lot Disclosure Average Weight (Kg) 18.3 18.1 Days to Ship (Days) 90 90 Shipment Average Weight (Kg) 107.5 113.9 Weight gain (Kg / day) 0.991 1.06 Feed Intake (kg / day) 2.58 2.63 Feed rate 2.73 2.52 ammonia 14 ppm 3 ppm Fly outbreak O △

As can be seen in Table 5 and Table 6, the diets treated with Candida pelliculosa SW001 (KFCC11481P) cultures showed excellent growth conditions, such as increased weight and feed intake, compared to no additions. In addition, it can be seen that the breeding environment is greatly improved, such as low ammonia concentration and insufficient fly occurrence.

Experimental Example 5 Weight Gain and Disease Occurrence of Korean Cattle

Twelve-month-old bigger cattle (Korean cattle) were divided into 20 control and 22 test trials, and the control diet was supplied with commercially mixed feed and rice straw, and the test plot was Candida pelliculosa SW001 (KFCC11481P) of Example 1 in the control feed. ) 0.1% culture was added. And the weight gain and disease occurrence was measured and the results are shown in Table 7.

division Starting weight (kg) Weight (kg) Weight gain (kg) Outbreak of disease Control 351 650 300 3 Test 348 710 355 One

As shown in Table 7, the increase in weight gain was significantly improved compared to the control, and the number of disease occurrences can be confirmed. Dietary diarrhea, bloat and urethral stones were seen in the control group, and urethral stones were found in the test group.

Experimental Example 6 Milk Flow, Milk Fat and Milk Protein

1 month and 4 months old cows were fed to 80 cows, and 2 months and 3 months old cows were fed with Candida pelliculosa SW001 (KFCC11481P) in Example 1 , Milk protein, somatic cell count and disease occurrence were investigated, and the results are shown in Table 8.

division Flow rate (kg) butterfat(%) Milk Protein (%) Somatic cell count (only) Disease Outbreaks Control 27.5 3.75 3.15 26 5 Test 30.5 3.92 3.22 18 One

As shown in Table 8, the flow rate, milk fat, and milk protein were all increased compared to the control, and the somatic cell count and the incidence of disease were reduced, and the milk grade and quality were improved. In the control group, diseases such as planting and mastitis occurred, and in the test group, diseases of the fourth place were developed.

Experimental Example 7 Grade of Beef Cattle

Candida pelliculosa SW001 (KFCC11481P) of Example 1 in a beef cattle self-TEM feeding farm using a compound feed, a sapphire chain, protein skin, beet pulp, beer foil, horse skin, cotton thread, pescuit, mixed hay, etc. Cultures were fed after fermentation for 24 hours after addition of 0.1%, grades for beef cattle were measured, and the results are shown in Table 9.

Rating One 1+ 1 ++ Head percentage A One 4 4 9 31.03 B 2 3 8 13 44.83 C 3 2 2 7 24.14 Head 6 9 14 29 percentage 20.69 31.03 48.28

As shown in Table 9, after six months, the first-class shipment rate of the 29 heads improved to 100%.

Example 8

In general, the silage manufacturing method uses a method of cutting plants into silos and suppressing them. However, in recent years, since the development of agricultural machinery, balers are made of large round balers without cutting materials, and then wrapped with vinyl and stored. It is called raw straw straw silos manufacturing technology by round bales. In other words, silage of fresh straw will add strain to the process of making balers. In the same manner as above, without additives, A product addition, Candida pelliculosa SW001 (KFCC11481P) culture of Example 1, Candida pelliculosa SW001 (KFCC11481P) culture and Example 1 of Candida pelliculosa ( Lactobacillus plantarum ) mixed with 10 ⁶ cfu / g of fresh straw and total barley silage, and 2 months after storage, the number of lactobacillus, fungal viable counts and palatability of rice straw and total barley silage were examined. The results for raw straw silage are shown in Table 10, and the results for total barley silage are shown in Table 11.

division Raw straw straw silage Lactobacillus (c.f.u / g) Mold (c.f.u / g) Palatability No additives 2.1 × 10 ⁷ 3.9 × 10 ² usually A company 1.8 × 10 ⁸ 1.4 × 10 ² good Candida Felicurosa 4.9 × 10 ⁸ 6.3 × 10 ¹ Very good Candida Felicurosa, mixed with lactic acid bacteria 6.3 × 10 ⁸ 3.5 × 10 ¹ Very good

division Chongbori Silage Lactobacillus (c.f.u / g) Mold (c.f.u / g) Palatability No additives 4.7 × 10 ⁷ 3.3 × 10 ² usually A company 3.5 × 10 ⁸ 1.2 × 10 ² good Candida Felicurosa 6.3 × 10 ⁸ 3.4 × 10 ¹ Very good Candida Felicurosa,
Lactobacillus mixed use 7.8 × 10 ⁸ 1.7 × 10 ¹ Very good

Table 10 and Candida pellets Kurosawa, as shown in Table 11 (Candida pelliculosa SW001) with lactic acid bacteria (Lactobacillus plantarum ) showed the highest number of lactobacillus and the lowest fungi in the mixed group. Secondly, Candida pelliculosa SW001 alone added good results.

Example 9 Fermentation Odor and Protein Content

Fermentation of grain by-products is being attempted to replace expensive feeds due to rising feed prices, but more than 50% water content is required for efficient cultivation of microorganisms. I'm doing it. 200 micrograms of rice bran, 2 kg of sugar, 40 liters of water, 200 ml of Candida pelliculosa SW001 culture medium in a microbial fermentation feed mixer (Daedong Tech) and incubated at 30 ° C. for 24 hours while stirring for 10 minutes each time Protein content was measured. The results are shown in Table 12.

division Fermentation odor Protein content No additives No special smell 12.6% Candida Felicurosa
Add 0.1% of culture Fermentation odor is strong 16.9.%

As shown in Table 12, the fermentation did not occur due to the low water content and the strong fermentation odor occurred in 0.1% of Candida pelliculosa SW001 cultures. 4.1% increase.

Example 10 Yeast Bacteria Fermentation Comparison

Microbial fermentation feed mixer (Daedong Tech Co., Ltd.), respectively bran to 4 200kg, sugar 2kg, put 40 liters of water with no additives, baking yeast (Saccharomyces cerevisiae ) Add 200ml of culture medium, saccharomyces cerevisiae ) 200ml addition, 200 ml of Candida pelliculosa SW001 culture medium was added, and the fermentation state and protein content were measured after incubation at 30 ° C. for 24 hours while stirring for 10 minutes at 1 hour. The results are shown in Table 13.

division Fermentation status Protein content after fermentation No addition No fermentation odor 12.5% Baking Yeast
(Saccharomyces cerevisiae) No fermentation odor 12.8% Rice wine yeast
(Saccharomyces cerevisiae) No fermentation odor 13.1% Candida Felicurosa
(Candida pelliculosa SW001) Fermentation odor is strong 17.1%

As shown in Table 13, fermentation odor did not occur in baker's yeast and makgeolli yeast, and there was no change in protein content due to no increase in cell mass, but fermentation odor was strong in addition of Candida pelliculosa SW001. After the incubation, the protein content increased by more than 5% due to the increase of the cells due to fermentation.

Experimental Example 11

Saccharomyces cerevisiae ) and rice wine yeast ( Saccharomyces) cerevisiae ) and Candida pelliculosa SW001 bacteria were cultured in liquid phase, and the number of viable cells was measured after incubation at 10 ° C. for 24 hours at 0.1% inoculation in YM liquid medium.

And Saccharomyces cerevisiae ), Saccharomyces cerevisiae and Candida pelliculosa SW001 bacteria were incubated in a flask, respectively, and inoculated in 1 ml of rice bran and 10 g of sugar, inoculated in 1 ml each. After 148 hours incubation in ℃ incubator was measured for viable count.

The results are shown in Table 14.

division After 48 ℃ incubation
Viable cell count (cfu / ml) Water content 30%
Live after 48 hours incubation at 28 ℃
Number of bacteria (cfu / ml) Baking Yeast
(Saccharomyces cerevisiae) 4.5 × 10 ⁵ 7.8 × 10 ⁵ Rice wine yeast
(Saccharomyces cerevisiae) 3.9 × 10 ⁵ 6.2 × 10 ⁵ Candida Felicurosa
(Candida pelliculosa SW001) 2.3 × 10 ⁷ 8.5 × 10 ⁷

Baking yeast, sake yeast at a low temperature of 15 ℃ as shown in Table 14, but maintaining the number of bacteria added to the growth in stationary state, Candida pellets Kurosawa (Candida pelliculosa SW001) is 2.3 × 10 ⁷ exhibited a cfu / ml. Therefore, it was found that growth is possible even at a low temperature of 15 ℃.

At 30% moisture content, the baker's yeast and makgeolli yeast maintained the growth of stagnant bacteria, but Candida pelliculosa SW001 showed 8.5 × 10 ⁷ cfu / ml. Therefore, it was found that growth is possible even in 30% less moisture.

Korea Microbial Conservation Center (Domestic) KFCC11481 20100224

Claims (5)

Candida pelliculosa SW001 (KFCC11481P).
Candida pelliculosa (Candida pelliculosa) SW001 (KFCC11481P) comprising a microbial for livestock feed comprising a.
A silage additive comprising Candida pelliculosa SW001 (KFCC11481P).
Candida pelliculosa (Candida pelliculosa) SW001 (KFCC11481P) comprising a manure odor improvement agent.
Agricultural product fermentation agent comprising Candida pelliculosa SW001 (KFCC11481P)