KR100883515B1 - Agitator and microbe cultivation apparatus using the same - Google Patents

Abstract

A stirring unit for a bioreactor is provided to operate effectively attracting force between south pole and N pole magnet and to omit separate a waterproof process by isolating an activeness magnetic driving part and a culture tub. A stirring unit for a bioreactor contains a motor(71) installed outside a culture tub, an activeness magnetic driving part(73) having a plurality of magnet type partitions(73b) arranged at outer surface of the culture tub and rotated by driving force of a motor, a passivity magnetic driving part(75) having a plurality of magnet type partitions(75b) arranged at a surface facing to the activeness magnetic driving part, a supporting shaft(77) of which one end is fixed to the culture tub, a rotary hollow shaft(78) in which one end is fixed to the passivity magnetic driving part and which rotates with the passivity magnetism fixing unit and a plurality of impeller blades(79) agitating microorganism and blood culture fluid and rotating by receiving torque from the passivity magnetic driving part.

Description

Agitation unit and microbe incubator using the same {Agitator and Microbe Cultivation Apparatus Using the Same}

The present invention relates to a stirring unit and a microorganism incubator using the same, and in particular, it is possible to effectively act the suction force between the N pole and the S pole magnet piece by facing a large area between the magnet pieces facing the active and driven magnetic drive unit, As the magnetic drive unit is disposed inside the culture tank to completely separate the active magnetic drive unit and the culture tank from the outside of the culture tank, a separate waterproof treatment can be omitted, and thus, a stirring unit having a simple structure and a microorganism incubator using the same can be omitted. will be.

Recently, agriculture, livestock, and fisheries using various microorganisms have been developed and widely used. As a method of using these microorganisms, photosynthetic bacteria, lactic acid bacteria, Bacillus bacteria, yeast bacteria, cyanobacteria, and the like are diluted in water, followed by seed disinfection, It is a method of spraying soil, irrigation or foliar spraying of livestock and feeding livestock, which promotes crop production, prevents pests, promotes growth in livestock, prevents disease, improves meat quality and reduces odor and pollution of feces. In addition, it is used to inhibit the growth of pathogens, to help beneficial bacteria and to obtain the effect of suppressing harmful bacteria.

In addition, microorganisms can be used in the field of water purification, organic waste fermentation agent, various industrial waste purification, and when used in aquaculture, water purification, suppressing the occurrence of pathogens, improving disease resistance.

In addition, conventionally, after the high temperature sterilization process as described above, in order to enter into a rapid microbial culture, a separate heat is released to lower the temperature of the culture medium inside the high temperature sterilized culture tank to about 37 ° C., which is an appropriate temperature for microbial culture. By running only a circulation pump without a means to circulate the hot sterilized culture medium continuously in the culture tank.

Therefore, through such a conventional circulating cooling method, not only takes a long time to lower the high-temperature cultured liquid to an appropriate temperature, but also lowers the temperature by simply circulating, so that the external temperature is, for example, 40 ° C. or more. There was a problem that can not maintain the proper temperature greatly affected by the external temperature.

Moreover, the conventional microbial incubator lowers the temperature by simply circulating the circulating pumped medium during microbial cultivation, so if the external temperature is 40 ° C. or higher, for example, the microorganism incubator cannot maintain the proper temperature so that the desired culture cannot be achieved. There was.

In addition, the conventional microbial incubator is agitated together with the cooling of the culture medium through the circulation pump, such that when simply circulating the culture medium in one direction, a region where the stirring is smooth and an area where it is not is generated. There was a problem that the stirring efficiency was lowered.

Moreover, in the conventional microbial incubator, since the microbial culture medium exhibits a strong acid property of about pH 3, the circulation pump also has a problem of using an expensive pump that can withstand it.

In addition, in the related art, an impeller is driven by using a motor installed on the upper side of the culture vessel for circulation, uniform mixing and dilution for cooling the cultured liquid, or as disclosed in Korean Patent Publication No. 193-8967. The impeller inside the culture tank is driven by using a self-coupling drive device arranged on the lower side.

First, in the upper drive type in which the motor is disposed on the upper side, there is a problem in that the mechanical seal is to be dismantled along with the trouble of separating the drive motor installed in the upper cover every time the inside of the culture tank is washed.

In addition, the microbial incubator of Patent Publication No. 193-8967 is a method in which a plurality of magnets are magnetically coupled in a radial manner so that the opposing areas between the magnets facing each other are small, so that the magnetic coupling force is weak. Difficult, since the magnetic drive device is a structure that extends into the culture tank, a complex waterproof structure was required, which makes the structure of the microbial incubator complicated, and there was a problem that the cleaning of the culture tank made before the microbial culture was not smooth.

The present invention, in order to solve the above problems, as a large area between the magnet pieces facing the active and driven magnetic drive unit facing the large area, the stirring unit which can effectively act the suction force between the N pole and S pole magnet pieces and using the same The purpose is to provide a microbial incubator.

Another object of the present invention is to separate the active magnetic drive unit and the culture vessel outside of the culture tank by placing the passive magnetic drive unit inside the culture tank can be omitted a separate waterproof treatment, thereby stirring with a simple structure It is to provide a unit and a microorganism incubator using the same.

In order to achieve the above object, the present invention is a stirring unit for a microbial incubator for culturing after mixing and cultured by mixing the culture medium and the microorganisms put into the culture tank, a motor installed outside the culture tank; An active magnetic drive unit which rotates by receiving the driving force of the motor and has a plurality of magnet pieces arranged on an outer surface of the culture tank; A driven magnetic drive unit arranged inside the culture tank and having a plurality of magnet pieces arranged on a surface facing the active magnetic drive unit so as to be magnetically coupled to the rotation of the active magnetic drive unit; A support shaft having one end fixed to the culture tank; A rotary hollow shaft rotatably coupled to an outer circumference of the support shaft and having one end fixed to the driven magnetic driving unit and rotating together with the driven self-fixing unit; And a plurality of impeller blades coupled to the rotating hollow shaft and rotating while receiving rotational force from a driven magnetic drive unit, to agitate the microorganism and the cultured solution.

The stirring unit is installed inclined at a position in which the rotating shaft of the impeller is biased from the center of the lower end of the culture tank, the drain for the collection of the culture medium is completed is disposed in the center of the lower center of the culture tank, or the stirring unit is a rotating shaft of the impeller It is installed in the center of the bottom of the culture tank, the drain for the collection of the culture medium is completed can be disposed in a position biased from the center of the bottom of the culture tank.

The active magnetic driving unit includes an annular rotating body which is a nonmagnetic body coupled to the driving shaft of the motor, wherein the rotating body has the plurality of magnet pieces arranged radially with respect to the center of the rotating body on one surface of the rotating body. The driving unit includes an annular rotating body which is a nonmagnetic body fixed to the rotating hollow shaft, and the rotating body preferably has a plurality of magnet pieces arranged radially with respect to the center of the rotating body on one surface thereof. In this case, it is preferable that each of the plurality of magnet pieces N poles and S poles arranged in the active and driven magnetic driving units are alternately arranged.

Since the rotating body of the active magnetic drive unit and the drive shaft of the motor are coupled in a key coupling manner, mutual rotation of the active magnetic drive unit and the rotating body can be suppressed.

Each of the active and driven magnetic driving units may include a plastic cover covering the plurality of magnet pieces so that the plurality of magnet pieces are not exposed.

It is preferable that each rotating body of the said active and driven magnetic drive part is a metal material which has corrosion resistance.

The plurality of impeller blades are arranged at a predetermined interval up and down on the rotary hollow shaft, a plurality of holes may be formed to evenly mix the particle size of the oxygen to be evenly mixed with the culture medium. In addition, the plurality of impeller blades may be arranged to be inclined at a predetermined angle so as to selectively generate a vortex.

The culture vessel has a lower surface convex, and the active magnetic drive portion preferably forms a groove corresponding to the curved surface of the culture vessel in the central portion of the side facing the culture tank so as to be disposed close to each other the active and driven magnetic drive portion. Do.

The stirring unit may be inclined toward one side with respect to the longitudinal direction of the culture tank.

The microbial incubator including the stirring unit includes a microbial incubator for irradiating light and agitating microorganisms and a culture medium to culture microorganisms introduced into the culture tank, the microorganism incubator having an open top and corrosion resistance; A cover for opening and closing an upper portion of the culture tank; At least one lighting unit installed on the cover to irradiate light into the culture tank; And an impeller rotatably installed on a support shaft installed at a lower end of the culture vessel, and a stirring unit installed in the culture vessel to circulate the cultured liquid and the microbial strain and the cultured liquid to the cooling passage. The stirring unit is an impeller rotatably installed on the support shaft installed in the lower end of the culture tank; A motor installed outside the culture tank; And a pair of magnetic drives coupled magnetically to transfer the rotational force of the motor to the impeller.

The pair of magnetic drive units are directly rotated by receiving the rotational force of the motor and an active magnetic drive unit in which a plurality of magnet pieces are arranged on one surface facing the culture tank; And a driven magnetic drive unit disposed inside the culture tank and having a plurality of magnet pieces arranged on a surface opposite to the active magnetic drive unit to rotate in response to rotation of the active magnetic drive unit.

Further, the microorganism incubator is installed on one side of the culture tank so as to surround the cooling passage and the cooling unit, air cooling housing through which the outside air or cooling air passes through the heat exchange with the cooling unit by a blower; .

As described above, in the present invention, since the active and driven magnetic driving parts face a large area between the opposing magnet pieces, suction force can effectively act between the N pole and the S pole magnet pieces, thereby rotating the impeller blades with a large driving torque. There is an advantage that can improve the stirring efficiency of the culture medium.

In addition, the magnetic drive unit of the present invention is completely isolated through the culture tank can omit the waterproofing structure to keep the structure of the microbial incubator simple, and also maintain the simple structure has the effect of reducing the element of failure Of course, there are advantages of high durability and low material cost.

Hereinafter, with reference to the accompanying drawings will be described in detail the configuration of the microorganism incubator according to an embodiment of the present invention.

1 to 3 is a front view, a plan view and a side cross-sectional view showing a microbial incubator according to an embodiment of the present invention, Figure 4 is an enlarged cross-sectional view showing the outlet side of the culture medium cooling passage, Figure 5 is an overflow Figure 6 is an enlarged cross-sectional view showing a structure, Figure 6 is an enlarged cross-sectional view showing a lighting unit, Figure 7 is an enlarged cross-sectional view showing a coupling structure of the cooling unit installed in the culture medium cooling passage, Figure 8 is a view showing a magnetic coupler, Figure 9 is An enlarged cross-sectional view showing the first and second magnetic drive portions of the stirring unit.

The microbial incubator 1 of the present embodiment includes a body 2, a cover 9 provided on the upper side of the body 2, and an illumination for irradiating light into the culture tank 10 inside the body 2. A unit 30, a cooling unit 50 for cooling the cultured liquid in the culture tank 10 to an appropriate temperature, and a stirring unit 70 for stirring the cultured liquid.

The body 2 supports the culture tank 10 and is provided with a cover 9 for opening and closing the upper portion of the culture tank 10 on the upper side. In addition, the body 2 is provided with an installation space (2a) for installing various electronic devices (not shown) and oxygen supply device (5) for operating the microorganism incubator (1) below the culture tank (10).

The cover 9 is provided with a medium inlet 9a and a microbial strain inlet 9b for injecting a culture medium and a microbial strain into the culture tank 10, respectively, and a plurality of lighting units 30 are installed. . In this case, it is preferable that the medium inlet 9a and the microbial strain inlet 9b are sealably coupled to the cover 9 so that outside air does not flow into the culture tank 10 after the medium and microbial strain are introduced.

When the culture tank 10 is sterilized by heating a culture medium mixed with water and a medium at a high temperature before culture, the culture tank 10 has a predetermined thickness so that the culture tank 10 is not damaged or deformed due to the high temperature generated at this time. It is made of an opaque metal material, for example, stainless steel, having corrosion resistance to heat and strong acidic cultures. In addition, the culture tank 10 has a space portion 10a into which the culture medium is charged. The upper part of the culture tank 10 is filled with water, a medium, and a microbial strain, and is opened so that light can be irradiated with the culture medium through the lighting unit 30. do. Accordingly, even when the microorganism is attached to the wall of the culture tank 10 during the culture, the illumination unit irradiates light through the open upper part of the culture tank 10, unlike the prior art, the uniform amount of light continuously during the culture time Irradiation can be prevented to reduce microorganisms due to reduced dose.

The culture tank 10 has a culture medium cooling passage 13, an air cooling housing 14, an overflow pipe 15, a sieve heater 16, a water inlet 17, a cleaning port 18, a confirmation window 19 And a drain pipe 20.

The culture medium cooling passage 13 undergoes a pretreatment process of removing various germs inside the culture tank 10 by heating the culture medium mixed with water and the medium to high temperature sterilization before starting the microbial culture.

Thereafter, the high temperature sterilized culture medium is formed so as to be isolated from the space portion 10a on one side of the culture tank 10 so as to surround a part of the culture tank 10 to cool down to an appropriate culture temperature as soon as possible. The culture medium cooling passages 13 are formed with inlets 13a and outlets 13b on both sides of the culture medium cooling passages 13 and the culture tank 10, respectively. In this case, as shown in FIG. 4, the outlet port 13b is formed to narrow toward the outlet port 13b from the culture medium cooling passage 13 so as to increase the flow velocity of the mixed solution therethrough to form a vortex in the culture tank 10.

In addition, the guide plate 13c is disposed in the space portion 10a adjacent to the discharge port 13b so as to improve the turning force of the vortex injected at a high flow rate by the discharge port 13b. The guide plate 13c has a curvature corresponding to the curvature of the culture tank 10 in order to maintain the circulation of the cultured liquid of the space portion 10a satisfactorily. On the other hand, the culture medium cooling passage 13 of the present embodiment, of course, in addition to the above-described shape, it is also possible to have a pipe shape in which both ends communicate with the culture tank 10, respectively.

The air cooling housing 14 is formed at one side of the culture tank 10 to surround the culture medium cooling passage 13 and the plurality of cooling units 30 together. The air cooling housing 14 is formed with an air inlet 14a through which cooling air is introduced on one side and an air outlet 14b through which the hot air heat-exchanged with the cooling unit 30 is passed through the air cooling housing 14 on the other side. do. In this case, the air-cooled housing 14 is formed with a repair window 14c for opening the air-cooled housing 14 to maintain the cooling unit 30 disposed inside.

The overflow pipe 15 is formed on one side of the culture tank 10 so as to discharge bubbles to the outside of the culture tank 10 when bubbles are generated when cultured aerobic microorganisms such as yeast overflows the appropriate level. In this case, as shown in FIG. 5, one end of the overflow pipe 15 communicates with the overflow outlet 10c and the other end is bent downward. In addition, the overflow pipe 15 has an opening / closing valve 15a seated on the valve seat 10d around the overflow outlet 10c on the inner side to elastically open and close the overflow outlet 10c by the spring 15b. The on-off valve 15a opens the overflow outlet 10c only when the culture liquid overflows to prevent various bacteria present in the outside air from flowing into the culture tank 10 through the overflow outlet 10c.

Sheath heater (16) is installed on one side of the culture tank (10), and before the cultivation is heated to the sterilization of the culture medium by heating the culture medium added to the culture tank 10 to a high temperature of about 95 ℃ Is used. In this case, since the culture tank 10 is made of a material having heat resistance, even if the sheath heater 16 is operated at a high temperature, the culture medium can be sterilized without being affected. In addition, the siege heater 16 may also serve to maintain the culture medium so that the culture medium does not fall below a suitable temperature for culture by the external environment. The sheath heater 16 is installed in a state in which a part penetrates through the culture tank 10 and penetrates into the space portion 10a in order to raise the temperature of the culture medium in direct contact with the culture medium. In relation to the operation of the sheath heater 16, the culture tank 10 is provided with a temperature sensor 16a for sensing the temperature of the culture medium.

The water inlet 17 is installed on one side of the culture tank 10 to supply water for culturing the microorganisms cultured in the culture tank 10. The cleaning tool 18 is formed at one side of the culture tank 10 in a predetermined size so that a predetermined cleaning tool can be put into the culture tank 10 and cleaned. Confirmation window 19 is formed on one side of the culture tank 10 so that the user can directly visually check the inside of the culture tank 10 during the operation of the microorganism incubator (1).

Drain pipe 20 is installed so that one side is in communication with the lower portion of the culture tank 10, the other side is protruded to the outside of the body (2). The user may discharge the microorganisms cultured in the culture vessel 10 from the culture vessel 10 through the drain pipe 20. In this case, it is preferable that the drain pipe 20 is installed at the center of the lower part of the culture tank 10 which is substantially hemispherical so that all of the microorganisms are discharged without remaining in the culture tank 10. In this connection, the stirring unit 70 to be described later is installed on one side of the drain pipe 20 so as not to interfere with the drain pipe 20.

As shown in FIG. 6, a plurality of lighting units 30 are installed in the cover 9, and each of the housing 30a, the lamp 31, the reflector plate 33, the transparent body 35, the infrared ray blocking film 36, and the like. And a blower 37. The lighting unit 30 can of course be expanded according to the increase of the culture tank capacity.

The housing 30a includes an inner portion 30b that enters the cover 9 and an exposed portion 30c that is exposed outside the cover 9.

A lamp 31 is installed in a part 30b of the housing 30a, and a reflecting plate 33 reflecting light to irradiate light emitted from the lamp 31 toward the culture tank 10 around the lamp 31 is provided. Is installed. In addition, a transparent body 35 is installed under the portion 30b of the housing 30a to protect the lamp 31 and to transmit the light irradiated from the lamp 31 to the culture tank 10. The transparent body 35 may be made of, for example, glass made of a light transmissive material, tempered glass, or a resin having predetermined heat resistance.

On the other hand, the infrared ray blocking film 37 for blocking the infrared rays included in the light is attached to the transparent body (35).

Furthermore, an air suction hole 30d is formed in the exposed portion 30c of the housing 30a to allow external air to flow in one side thereof, and the opposite side sucks the external air through the air suction hole 30d and the housing 30a. The blower 37 for forcibly discharging to the outside via the inside is installed. The air cooling system of the lighting unit 30 is to prevent the heat generated by the lamp 31 from affecting the culture medium, as well as to prevent overheating of the lamp 31 exposed to high temperature for a long time during the culture.

A plurality of cooling units 50 are installed along the culture medium cooling passages 13 in the air cooling housing 14, and each of the cooling units 50 has a cooling rod 51, a spiral heat radiation fin 53, and a sealing nut 55. It includes.

Referring to Figure 5, the cooling rod 51 is made of a metal material with excellent heat transfer, the lower end penetrates the culture medium cooling passage through the socket 52 installed in the culture medium cooling passage 13 so as to be in direct contact with the culture medium. 13) is installed.

The helical radiating fins 53 are coupled in a spiral direction along the cooling rods 51 to widen the area where the cooling unit 50 contacts the cooling air. In addition, the spiral heat sink fin 53 is a relatively high temperature compared to the upper end of the cooling rod 51 while the cooling air passing through the air cooling housing 14 is rotated from the bottom of the cooling rod 51 to the top along the spiral heat radiation fin (53). By transmitting the temperature of the lower portion of the cooling rod 51 to the upper side of the cooling rod 51, the cooling performance of the cooling unit 50 as a whole can be improved.

The sealing nut 55 is screwed to the cooling rod 51 to rotate along the cooling rod 51 to be fastened to the socket 52, thereby not only fixing the cooling rod 51 to the culture medium cooling passage 13. The airtightness of the insertion hole 13d formed for inserting the cooling rod 51 can be maintained.

The stirring unit 70 includes a geared motor 71, an active magnetic drive unit 73, a driven magnetic drive unit 75, a support shaft 77, a rotary hollow shaft 78, and a plurality of impeller blades 79. . In this case, the rotary hollow shaft 78 and the plurality of impeller blades 79 form an impeller.

The stirring unit 70 is agitated by forced circulation of the culture medium through a magnetic drive, unlike the natural circulation method using a circulation pump of the conventional microbial incubator for effective agitation of the culture medium and microorganisms introduced into the culture tank 10. Is done.

The geared motor 71 is disposed in the installation space 2a of the body 2 to impart rotational force to the active magnetic drive unit 73. By arranging the motor 71 in the lower portion of the outer side of the culture tank 10 as described above, the cleaning of the culture tank 10 can be facilitated, and the position of the motor 10 can be installed at the lower end of the culture tank 10. Alternatively, when the drain pipe 20 is located at the lower end of the culture tank 10 as shown in FIG. 1, the drain pipe 20 is inclined toward one side or the other side so as not to interfere with the drain pipe 20.

In addition, the stirring unit 70 is a rotary hollow shaft 78 of the impeller is installed perpendicular to the center of the bottom of the culture tank 10, the drain for the collection of the culture medium is completed, the center of the bottom of the culture tank 10 May be disposed in a position biased from.

Furthermore, the motor 71 transmits the rotational force to the drive shaft 71a in a state in which the rotational force is decelerated through the gearbox 72a.

The active magnetic drive part 73 is provided with the annular rotating body 73a which consists of a nonmagnetic body which has corrosion resistance, such as stainless steel and aluminum, as shown in FIG. The rotating body (73a) is rotatably supported by a fixing bracket (72) installed in the body (2) and the center of the mutual key coupling method through the drive shaft (71a) and the key (74a) of the geared motor (71) As it is coupled to the rotation of the drive shaft (71a) is suppressed, it is fixed using the set screw (74b).

In addition, the active magnetic drive unit 73 is completely isolated from the culture tank 10 as it is installed outside the culture tank 10 can be omitted a separate waterproof treatment. In addition, the microbial incubator has a simple structure, which can greatly reduce the mechanical failure factor.

In addition, as shown in FIG. 7, on one surface of the rotating body 73a facing the driven magnetic driving unit 75, a plurality of magnet pieces 73b are arranged at equal intervals in the circumferential direction around the driving shaft 71a. In the magnet piece 73b, the N pole and the S pole are alternately arranged as shown in FIG. Furthermore, as shown in FIG. 8, the plurality of magnet pieces 73b are stably mounted to the rotating body 73a through the plastic cover 73c.

The driven magnetic drive unit 75 is made of a nonmagnetic material similar to the active magnetic drive unit 73, and includes a rotating body 75a rotatably installed below the culture tank 10, and faces one surface of the active magnetic drive unit 73. In the circumferential direction with respect to the center, a plurality of magnet pieces 75b are arranged at equal intervals. In this case, the plurality of magnet pieces 75b are alternately arranged with the N poles and the S poles similarly to the active magnetic drive portion 73, and are mounted to the rotating body 75a by the plastic lid 75c.

The support shaft 77 has a lower end 77a fixed to the bottom of the culture tank 10 by welding, and the other end thereof is disposed at a predetermined height inside the culture tank 10, and the drive shaft 71a of the geared motor 71 is fixed to the support shaft 77. Arranged coaxially. In this case, as the first bearing 76a is installed between the support shaft 77 and the rotating body 75a of the driven magnetic drive part 75, the rotating body 75a is rotatably coupled to the supporting shaft 77. do.

The rotary hollow shaft 78 is coupled to the support shaft 77 with the inner surface having a predetermined interval with the outer circumference of the support shaft 77. At this time, the rotary hollow shaft 78 is rotatably installed on the support shaft 77 by the second bearing 76b (see FIG. 3) disposed above the support shaft 77. In addition, the rotary hollow shaft 78 has a lower end 78a fixed to the upper surface of the rotating body 75a of the driven magnetic drive unit 75, and thus the rotating hollow shaft 78 rotates together with the rotating body 75a.

The plurality of impeller blades 79 are disposed at predetermined intervals up and down on the rotary hollow shaft 78. Such a plurality of impellers (79) to form a plurality of holes (79a) to help in culture to crush the particle size of oxygen supplied from the lower side through the oxygen supply nozzle (6) to be evenly mixed in the culture medium. have. In this case, the impeller blades 79 may be disposed to be inclined at a predetermined angle so as to selectively generate vortices, and of course, the shape of the impeller blades 79 may be modified.

On the other hand, since the active and driven magnetic drive unit 73,75 is preferably close to the mutually opposite distance, the active magnetic drive unit 73 is the curved surface of the culture tank 10 in the central portion of the upper side facing the culture tank 10 It is preferable to form grooves 73d corresponding to and to arrange the grooves 73d close to each other.

It describes the action of the microorganism incubator 1 according to an embodiment of the present invention configured as described above.

First, after the water and the medium is introduced into the culture tank 10, the siege heater 16 is operated to heat the culture medium to a high temperature of about 95 ℃ for sterilization of the culture medium before the culture. After sterilization is performed, the cooling unit 50 and the stirring unit 70 are cooled to a temperature suitable for incubation.

Subsequently, the microorganism strain to be cultured is introduced through the microorganism strain inlet 9b, and then the medium inlet 9a and the culture medium inlet 9b are closed in a sealed state and culture for the culture medium is started.

Thereafter, when the stirring unit 70 is turned on, the geared motor 71 is driven while the active magnetic drive unit 73 connected to the drive shaft 71a rotates. Accordingly, when magnets of opposite polarities are opposed between the plurality of magnet pieces 73a of the active magnetic drive part 73 and the plurality of magnet pieces 75a of the driven magnetic drive part 75 corresponding thereto, the attraction force is reduced. As it occurs, the driven magnetic drive unit 75 rotates about the support shaft 77.

The driven magnetic drive unit 75 transmits a rotational force to the rotating hollow shaft 78 to rotate the plurality of impeller blades 79. Accordingly, the plurality of impeller blades 79 can enhance the culture conditions of the microorganisms compared to the conventional natural circulation by forcibly stirring the culture medium and the microorganisms injected into the space portion 10a of the culture tank 10.

On the other hand, the cultured liquid forcibly circulated in the culture tank 10 by the stirring unit 70 is circulated in the space (10a) while a part is introduced into the cooling passage (13). Subsequently, the cultured liquid contacts the plurality of cooling rods 51 protruding into the cooling passage 13 while passing through the cooling passage 13.

The plurality of cooling rods 51 absorb the heat transferred from the high temperature culture medium, and the heat absorbed in this way is transferred to the spiral heat sink fins 53. In this case, the air passing through the air-cooled housing 14 moves along the helical heat dissipation fins 53 to the upper side of the plurality of cooling rods 51, and thus the plurality of cooling rods 51 are cooled.

As a result, while the heat exchange is continuously made through a plurality of cooling units 50, the temperature of the culture medium is gradually reduced. Accordingly, the culture medium at which the temperature is lowered is introduced into the culture tank 10 at a high speed through the outlet 13b of the cooling passage 13, which is gradually narrowed, and forms a vortex, and at the same time, the turning force by the guide plate 13c. This provision promotes circulation of the cultured liquid.

On the other hand, the microorganism incubator 1 of the present invention continuously detects the culture medium temperature in the culture tank 10 through the temperature sensor 16a, the low temperature of the outside air or a plurality of cooling unit 50 of the culture medium When the temperature is sensed below the microorganism culture temperature, it is possible to operate the siege heater 16 to maintain the temperature of the culture medium to the optimum temperature for culturing the microorganism.

The microorganisms are cultured through the light irradiated from the plurality of lighting units 30 during the predetermined culture period. On the other hand, when the microorganism is an aerobic microorganism such as yeast, bubbles may occur and the culture medium may overflow above the proper level. In this case, in order to discharge the overflowed culture liquid to the outside of the culture tank 10, the opening and closing valve 15a opens the overflow outlet 10c of the culture tank 10, and then the discharge of the culture solution is completed and at the same time the spring 15b. ), The overflow outlet 10c is closed again.

When the culture of the microorganisms is completed, the drain pipe 20 is opened to discharge the cultured microorganisms to the outside of the culture tank 10. Then, when cleaning the culture tank 10, the cover 9 can be opened and the upper part of the culture tank 10 can be opened for easy cleaning.

As described above, in the present invention, the magnetic coupling type agitation unit 70 is used to stir the cultured liquid, and the driving device installed outside the lower end of the culture tank 10 has a driven magnetic drive unit installed inside the culture tank. The impeller 79 can be rotated by rotating in a coupling manner, so that the impeller 79 is rotated in a state where the culture tank 10 is completely sealed, and thus, the conventional method for the transmission of rotational power is achieved. It does not require the same complex sealing structure.

In addition, by installing the stirring unit 70 at the lower end of the culture tank 10, it is possible to easily install the lighting unit on the cover of the culture tank top, even when cleaning the culture tank without dismantling the installed motor Only the cover can be opened and cleaned.

In the present invention, by using a plurality of impeller blades 79 of the stirring unit 70 forcibly circulated the culture medium to be cultured and the culture medium and the microorganisms can be uniformly stirred in all zones. In addition, such a forced circulation system can also smoothly flow into and out of the cooling passage 13, which also helps the cooling of the cultured liquid.

Furthermore, in the present invention, by installing the stirring unit 70 inclined at a position offset from the center of the impeller 79, the drain for the collection of the culture medium is completed in the center of the bottom of the culture tank 10 It became possible to deploy. As a result, in the present invention, the lower end of the culture tank 10 is formed into a hemispherical type, and the microbial culture is naturally collected into the drain disposed at the center of the lower end of the culture tank 10, and the discharge is accomplished, thereby completing the culture solution. It is possible to get as much as possible without wasting.

In addition, the microbial incubator 1 according to an embodiment of the present invention, as a plurality of lighting units 30 are installed on the upper side of the culture tank 10, the light is cultured from the upper side of the culture tank 10 Since it is irradiated to the inside of the tank 10, it is possible to continuously irradiate a uniform amount of light to the microorganisms during the microbial culture time to prevent the microbial culture rate is reduced.

In addition, the present invention is the culture tank 10 is made of an opaque metal material, the sterilization by heating the culture medium through the siege heater 16 to sterilize without the need for providing a separate sterilization device itself Cultured liquid sterilization can be performed.

In addition, the present invention, as well as cooling the culture medium at a high speed through a plurality of cooling units 50, as well as increase the temperature of the culture medium through the siege heater 16 when the external temperature is low. Accordingly, it is possible to provide an optimal environment for culturing microorganisms by actively controlling the temperature regardless of the internal / external temperature, thereby maintaining a proper temperature suitable for culturing microorganisms at all times.

According to the present invention, the suction force between the N-pole and the S-pole magnet pieces can be effectively applied by opposing a large area between the magnet pieces facing the active and driven magnetic driving parts, and the driven magnetic driving parts are disposed inside the culture tank outside the culture tank. By completely separating the active magnetic drive unit and the culture tank of each other, a separate waterproof treatment can be omitted, and thus can be applied to a stirring unit having a simple structure and a microbial incubator using the same.

1 is a front view showing a microbial incubator according to an embodiment of the present invention,

2 is a plan view seen from the direction II shown in FIG.

3 is a side cross-sectional view taken along line III-III shown in FIG. 2;

4 is an enlarged cross-sectional view showing a portion IV shown in FIG.

5 is an enlarged cross-sectional view showing part V shown in FIG. 1;

6 is an enlarged cross-sectional view showing a lighting unit;

FIG. 7 is an enlarged cross-sectional view illustrating a portion VII shown in FIG. 3,

8 is a view showing an arrangement structure of the magnetic disposed on the magnetic coupler,

FIG. 9 is an enlarged cross-sectional view illustrating a portion V shown in FIG. 3.

* Description of Signs for Main Parts in Drawings *

2: body 5: oxygen supply

6: oxygen supply nozzle 9: cover

10: culture tank 20: drain pipe

30: lighting unit 50: cooling unit

70: stirring unit 71: geared motor

73: active magnetic drive unit 75: driven magnetic drive unit

77: support shaft 78: rotating hollow shaft

79: impeller blade

Claims (14)

In the microorganism incubator stirring unit 70 for culturing after mixing the culture medium and the microorganisms introduced into the culture tank 10, A motor 71 installed outside the culture tank; An active magnetic drive part 73 in which a plurality of magnet pieces 73b are arranged on an outer surface of the culture tank while being directly rotated by the driving force of the motor; A driven magnetic drive unit 75 disposed inside the culture tank and having a plurality of magnet pieces 75b arranged on a surface opposite to the active magnetic drive unit so as to be magnetically coupled to the rotation of the active magnetic drive unit 73; A support shaft 77 having one end fixed to the culture tank; A rotating hollow shaft (78) rotatably coupled to an outer circumference of the support shaft and having one end fixed to the driven magnetic driving unit (75) to rotate together with the driven self-fixing unit; And, A plurality of impeller blades 79 which are coupled to the rotary hollow shaft 78 and receive the rotational force from the driven magnetic driving unit 75 and stir the microorganisms and the culture medium; . According to claim 1, The stirring unit 70 is installed inclined at a position in which the rotary hollow shaft 78 of the impeller blade 79 is biased from the center of the lower end of the culture tank 10, the collection of the culture medium is completed Drain for the microbial incubator, characterized in that disposed in the center of the bottom of the culture vessel. The method of claim 1, wherein the stirring unit is a rotary hollow shaft 78 of the impeller blade 79 is installed perpendicular to the center of the bottom of the culture tank, the drain for the collection of the culture medium is completed from the center of the bottom of the culture tank Stirring unit for a microorganism incubator, characterized in that arranged in a biased position. The method of claim 1, wherein the active magnetic drive unit 73 And an annular rotating body (73a) which is a nonmagnetic material coupled to the drive shaft (71a) of the motor, wherein the plurality of magnet pieces (73b) are arranged radially with respect to the center of the rotating body on one surface , The driven magnetic drive unit 75 It includes an annular rotating body (75a) is a non-magnetic body is fixed to the rotary hollow shaft 78, the rotating body is a plurality of magnet pieces 75b on one surface is arranged radially with respect to the center of the rotating body Stirring unit for microorganism incubator, characterized in that. 5. The stirring apparatus for microbial incubator according to claim 4, wherein each of the plurality of magnet pieces (73b, 75b) arranged in the active and driven magnetic driving parts (73, 75) is arranged with N poles and S poles alternately. unit. The stirring unit for a microorganism incubator according to claim 4, wherein the rotating body (73a) of the active magnetic drive unit and the drive shaft (71a) of the motor are coupled in a key coupling manner. The method of claim 4, wherein the active and driven magnetic drive portion (73, 75) comprises a plastic cover (73c, 75c) covering the plurality of magnet pieces so that the plurality of magnet pieces (73b, 75b) are not exposed, respectively. Stirring unit for microbial incubator, characterized in that. The stirring unit for a microorganism incubator according to claim 4, wherein each of the rotating bodies (73a, 75a) of the active and driven magnetic driving units (73, 75) is a metal material having corrosion resistance. The method of claim 1, wherein the plurality of impeller blades 79 are arranged at a predetermined interval up and down on the rotary hollow shaft 78, a plurality of holes to finely crush the particle size of oxygen to be evenly mixed in the culture medium. Stirring unit for a microorganism incubator, characterized in that (79a) is formed. 10. The stirring unit of claim 9, wherein the plurality of impeller blades (79) are inclined at a predetermined angle to selectively generate vortices. The method of claim 1, wherein the culture tank 10 has a lower surface convex curved, In the active magnetic drive portion to form a groove (73d) corresponding to the curved surface of the culture tank in the central portion of the side facing the culture tank 10 so as to place the active and driven magnetic drive units (73, 75) in close proximity to each other Stirring unit for microbial incubator, characterized in that. In the microbial incubator for irradiating light and agitating the microorganism and the culture medium to cultivate the microorganisms put into the culture tank, Culture tank 10 made of a metallic material having an upper portion is open and corrosion resistance; A cover (2) for opening and closing an upper portion of the culture tank; One or more lighting units (30) installed on the cover to irradiate light into the culture tank; And It is provided with an impeller blade (79) rotatably installed on the support shaft (77) installed at the lower end of the culture tank (10), and is installed in the culture tank for stirring and circulating the culture medium and the microorganism strain and the culture medium. Including a stirring unit (70), The stirring unit 70 is An impeller blade (79) rotatably installed on a support shaft installed at a lower end of the culture tank; A motor 71 installed outside the culture tank; And And a pair of magnetic drives (73, 75) magnetically coupled to transfer the rotational force of the motor to the impeller blades (79). The method of claim 12, wherein the pair of magnetic drive parts (73, 75) An active magnetic drive unit 73 in which a plurality of magnet pieces 73b are arranged on one surface facing the culture tank while rotating by receiving the rotational force of the motor; And A driven magnetic driving unit 75 disposed inside the culture tank and having a plurality of magnet pieces 75b arranged on a surface facing the active magnetic driving unit so as to rotate in response to the rotation of the active magnetic driving unit. Microbial incubator, characterized in that. The method of claim 12, The support shaft (77), the impeller blade (79) is rotatably installed is microbial incubator, characterized in that disposed inclined at a position biased from the center of the bottom of the culture tank.

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