KR20100006019A - Lighting unit and microbe cultivation apparatus using the same - Google Patents

Abstract

PURPOSE: A microorganism culture apparatus comprising a light unit irradiating light to a test culture medium is provided to prevent culture rate reduction. CONSTITUTION: A microorganism culture apparatus comprises a lighting unit(30) irradiating light to microorganism in a culture bath. The lightning unit comprises: an insertion part, an exposure part, a housing having air inhalation hole(30d) and air discharging hole; a lamp(31) mounted to the opening or the housing; a reflection plate(33) installed around the lamp; a transparent body(35) for in the housing; an infrared ray protection film; and an blower(37).

Description

Lighting Unit and Microbe Cultivation Apparatus Using the Same}

The present invention relates to a microbial incubator, and in particular, by placing a stirring unit for the culture medium to the lower side of the culture tank, an illumination unit for irradiating light to the culture medium is installed on the cover disposed on the upper side of the culture tank to irradiate light to the microorganism The present invention relates to an illumination unit and a microbial incubator using the same, which can prevent the lowering of the microbial culture rate by efficiently performing the high temperature sterilization for sterilizing the culture medium using a metal culture tank.

Recently, agriculture, livestock, and fisheries using various microorganisms have been developed and widely used. As a method of using these microorganisms, photosynthetic bacteria, lactic acid bacteria, Bacillus bacteria, yeast bacteria, cyanobacteria, and the like are diluted in water, followed by seed disinfection, It is a method of spraying soil, irrigation or foliar spraying of livestock and feeding livestock, which promotes crop production, prevents pests, promotes growth in livestock, prevents disease, improves meat quality and reduces odor and pollution of feces. In addition, it is used to inhibit the growth of pathogens, to help beneficial bacteria and to obtain the effect of suppressing harmful bacteria.

In addition, microorganisms can be used in the field of water purification, organic waste fermentation agent, various industrial waste purification, and when used in aquaculture, water purification, suppressing the occurrence of pathogens, improving disease resistance.

Therefore, the present applicant has proposed a microbial incubator disclosed in the domestic patent registration No. 732193 so that ordinary farms, fish users can easily cultivate the microorganisms and use it stably for their purposes.

Such a microbial incubator has a housing, and in the center of the housing, a culture tank made of a transparent PC (polycarbonate) having a light-transmissive material is disposed, and an inner wall of the housing is provided for supplying light and heat necessary for microorganisms grown in the culture tank. Multiple irradiation lamps are installed. In this case, the plurality of irradiation lamps are disposed in front of the plurality of irradiation lamps infrared cut filter for blocking infrared rays.

In this case, the microbial incubator controls the internal temperature to maintain an appropriate temperature, for example, about 37 ° C. so that the microorganisms in the culture tank can be well cultured by photosynthesis.

On the other hand, in general, the microbial incubator is a pretreatment step of the microbial culture for the purpose of removing various germs inside the culture tank, the water is charged to the inside of the culture tank and subjected to high temperature sterilization by heating to a high temperature. However, it is difficult to carry out such a high temperature sterilization process in Patent Registration No. 712193 because a light-transmissive culture tank is used to receive light emitted from a plurality of irradiation lamps installed on the inner wall of the housing.

Therefore, in the related art, in addition to the microbial incubator, it is necessary to provide a separate sterilization apparatus for sterilization. As a result, the culture tank is required to be made of a metal material so that the high temperature sterilization process can be processed.

On the other hand, when culturing photosynthetic microorganisms through the conventional microorganism incubator as described above takes about 80 ~ 90 hours in optimal conditions, and in the case of lactic acid bacteria it takes about 40 hours in optimal conditions.

By the way, such a conventional microbial incubator is arranged around the culture tank so that a plurality of irradiation lamps irradiate light from the side of the culture tank, in order to absorb the light from the microorganisms in the culture tank toward the second half of the culture if the culture is performed for a long time The phenomenon of attachment to the inside of the culture vessel wall occurs. Accordingly, while the amount of light passing through the culture tank is reduced by the microorganisms attached to the culture tank wall, the survival number of the microorganisms of the entire culture tank is reduced, resulting in a decrease in the culture rate.

In order to solve the above problems, the illumination unit for irradiating the light to the culture medium by arranging the stirring unit for the culture medium to the culture medium to be disposed on the cover disposed above the culture tank to irradiate light to the microorganism It is an object of the present invention to provide a microorganism incubator using the lighting unit and the same that can be carried out efficiently to prevent a decrease in the culture rate of microorganisms.

Another object of the present invention is to provide a microorganism incubator that can easily perform high temperature sterilization for sterilization of the culture medium before culture using a microorganism culture as well as a metal culture tank itself.

In order to achieve the above object, the present invention is a lighting unit for a microorganism incubator comprising a cover for opening and closing the culture tank and the upper side of the upper side, the buried portion embedded in the cover to open and close the top of the culture tank A housing having an exposed portion exposed to the outside of the cover and having an air discharge hole at a position opposite to the air suction hole and the air suction hole through which the outside air is sucked into the exposed portion of the housing; A lamp installed in an embedding portion of the housing; A reflection plate installed around the lamp to reflect light into the culture tank; A transparent body installed below the embedded portion of the housing to transmit light emitted from the lamp; An infrared ray blocking film attached to the transparent body to block infrared rays included in light passing through the transparent body; And a blower installed in the air discharge hole in order to forcibly flow in and out the external air into the housing.

In addition, the present invention by irradiating light through the lighting unit to the microorganisms put into the culture tank using the lighting unit, it is possible to configure a microorganism incubator for culturing the microorganisms.

According to another feature of the invention, the present invention is a microorganism incubator for culturing microorganisms by irradiating light through a lighting unit to the microorganisms in the culture tank, the lighting unit is embedded in a cover for opening and closing the upper portion of the culture tank. A lamp for irradiating light is installed on a portion of the light source, and the remaining portion exposed to the outside of the cover includes an air suction hole through which external air flows into the lighting unit and air introduced into the lighting unit through the air suction hole. It provides a microorganism incubator characterized in that the blower for forcibly discharged to the outside of the lighting unit is installed.

The microbial incubator includes an impeller for stirring the culture medium; And a driving device installed at the lower side of the culture tank to rotationally drive the impeller.

The culture tank may be made of a metal material, for example, stainless steel.

The culture tank is equipped with a sieve heater for sterilization by heating the culture medium introduced into the culture tank to a high temperature.

The microbial incubator includes at least one cooling unit installed in a portion of the cooling passage when the culture medium is circulated in one direction along a cooling passage formed at one side of the culture tank to cool the culture medium by heat exchange; And an impeller rotatably installed on a support shaft installed at a lower end of the culture vessel, and a stirring unit installed in the culture vessel to circulate the cultured liquid and the microbial strain and the cultured liquid to the cooling passage. Can be.

In the present invention as described above, by placing a stirring unit for the culture medium to the lower side of the culture tank, an illumination unit for irradiating the light to the culture medium is installed on the cover disposed above the culture tank to efficiently radiate light to the microorganisms. It can be carried out to prevent the reduction of the culture rate of the microorganism.

In addition, the present invention can easily perform high-temperature sterilization for sterilization of the culture medium before cultivation using a microorganism cultivator itself as well as a metal culture tank and a sieve heater.

Hereinafter, with reference to the accompanying drawings will be described in detail the configuration of the microorganism incubator according to an embodiment of the present invention.

1 to 3 is a front view, a plan view and a side cross-sectional view showing a microbial incubator according to an embodiment of the present invention, Figure 4 is an enlarged cross-sectional view showing the outlet side of the culture medium cooling passage, Figure 5 is an overflow structure 6 is an enlarged cross-sectional view showing an illumination unit, Figure 7 is an enlarged cross-sectional view showing a coupling structure of the cooling unit installed in the culture medium cooling passages, Figure 8 is a view showing a magnetic coupler, Figure 9 is stirring An enlarged cross-sectional view showing the first and second magnetic drive units of the unit.

The microbial incubator 1 of the present embodiment includes a body 2, a cover 9 provided on the upper side of the body 2, and an illumination for irradiating light into the culture tank 10 inside the body 2. A unit 30, a cooling unit 50 for cooling the cultured liquid in the culture tank 10 to an appropriate temperature, and a stirring unit 70 for stirring the cultured liquid.

The body 2 supports the culture tank 10 and is provided with a cover 9 for opening and closing the upper portion of the culture tank 10 on the upper side. In addition, the body 2 is provided with an installation space (2a) for installing various electronic devices (not shown) and oxygen supply device (5) for operating the microorganism incubator (1) below the culture tank (10).

The cover 9 is provided with a medium inlet 9a and a microbial strain inlet 9b for injecting a culture medium and a microbial strain into the culture tank 10, respectively, and a plurality of lighting units 30 are installed. . In this case, it is preferable that the medium inlet 9a and the microbial strain inlet 9b are sealably coupled to the cover 9 so that outside air does not flow into the culture tank 10 after the medium and microbial strain are introduced.

When the culture tank 10 is subjected to a sterilization process for removing various bacteria by heating a culture medium mixed with water and a medium at a high temperature before cultivation, the culture tank 10 is not damaged or deformed by the high temperature generated at this time. It is made of an opaque metal material, for example, stainless steel, having heat resistance and corrosion resistance having a predetermined thickness. In addition, the culture tank 10 has a space portion 10a into which the culture medium is charged, and an upper portion of the culture tank 10 is opened so that light can be irradiated with the culture medium through the lighting unit 30 and the medium and the culture medium. Accordingly, even when the microorganism is attached to the wall of the culture tank 10 during the culture, the illumination unit irradiates light through the open upper part of the culture tank 10, unlike the prior art, the uniform amount of light continuously during the culture time Irradiation can be prevented to reduce microorganisms due to reduced dose.

The culture tank 10 has a culture medium cooling passage 13, an air cooling housing 14, an overflow pipe 15, a sieve heater 16, a water inlet 17, a cleaning port 18, a confirmation window 19 And a drain pipe 20.

The culture medium cooling passage 13 has a space portion 10a at one side of the culture tank 10 so as to surround a portion of the culture tank 10 to cool the high temperature cultured solution introduced into the culture tank 10 through the inlet 12. It is formed to be isolated from. The culture medium cooling passages 13 are formed with inlets 13a and outlets 13b on both sides of the culture medium cooling passages 13 and the culture tank 10, respectively. In this case, as shown in FIG. 4, the outlet port 13b is formed to narrow toward the outlet port 13b from the culture medium cooling passage 13 so as to increase the flow velocity of the mixed solution therethrough to form a vortex in the culture tank 10.

In addition, the guide plate 13c is disposed in the space portion 10a adjacent to the discharge port 13b so as to improve the turning force of the vortex injected at a high flow rate by the discharge port 13b. The guide plate 13c has a curvature corresponding to the curvature of the culture tank 10 in order to maintain the circulation of the cultured liquid of the space portion 10a satisfactorily. On the other hand, the culture medium cooling passage 13 of the present embodiment, of course, in addition to the above-described shape, it is also possible to have a pipe shape in which both ends communicate with the culture tank 10, respectively.

The air cooling housing 14 is formed at one side of the culture tank 10 to surround the culture medium cooling passage 13 and the plurality of cooling units 30 together. The air cooling housing 14 is formed with an air inlet 14a through which cooling air is introduced on one side and an air outlet 14b through which the hot air heat-exchanged with the cooling unit 30 is passed through the air cooling housing 14 on the other side. do. In this case, the air-cooled housing 14 is formed with a repair window 14c for opening the air-cooled housing 14 to maintain the cooling unit 30 disposed inside.

The overflow pipe 15 is formed on one side of the culture tank 10 so as to discharge bubbles to the outside of the culture tank 10 when bubbles are generated when cultured aerobic microorganisms such as yeast overflows the appropriate level. In this case, as shown in FIG. 5, one end of the overflow pipe 15 communicates with the overflow outlet 10c and the other end is bent downward. In addition, the overflow pipe 15 has an opening / closing valve 15a seated on the valve seat 10d around the overflow outlet 10c on the inner side to elastically open and close the overflow outlet 10c by the spring 15b. The on-off valve 15a opens the overflow outlet 10c only when the culture liquid overflows to prevent various bacteria present in the outside air from entering the culture tank 10 through the overflow outlet 10c.

Sheath heater (16) is installed on one side of the culture tank (10), it is possible to sterilize the culture medium by heating the culture medium added to the culture tank 10 before the culture to a high temperature of about 95 ℃. In this case, since the culture tank 10 is made of a material having heat resistance, even if the sheath heater 16 is operated at a high temperature, the culture medium can be sterilized without being affected. In addition, the siege heater 16 may also serve to maintain the culture medium so that the culture medium does not fall below a suitable temperature for culture by the external environment. The sheath heater 16 is installed in a state in which a part penetrates through the culture tank 10 and penetrates into the space portion 10a in order to raise the temperature of the culture medium in direct contact with the culture medium. In relation to the operation of the sheath heater 16, the culture tank 10 is provided with a temperature sensor 16a for sensing the temperature of the culture medium.

The water inlet 17 is installed on one side of the culture tank 10 to supply water for culturing the microorganisms cultured in the culture tank 10. The cleaning tool 18 is formed at one side of the culture tank 10 in a predetermined size so that a predetermined cleaning tool is put into the culture tank 10 and cleaned. Confirmation window 19 is formed on one side of the culture tank 10 so that the user can directly visually check the inside of the culture tank 10 during the operation of the microorganism incubator (1).

Drain pipe 20 is installed so that one side is in communication with the lower portion of the culture tank 10, the other side is protruded to the outside of the body (2). The user may discharge the microorganisms cultured in the culture vessel 10 from the culture vessel 10 through the drain pipe 20. In this case, it is preferable that the drain pipe 20 is installed at the center of the lower part of the culture tank 10 which is substantially hemispherical so that all of the microorganisms are discharged without remaining in the culture tank 10. In this connection, the stirring unit 70 to be described later is installed on one side of the drain pipe 20 so as not to interfere with the drain pipe 20.

As shown in FIG. 6, a plurality of lighting units 30 are provided in the cover 12, and the housing 30a, the lamp 31, the reflector plate 33, the transparent body 35, the infrared ray blocking film 36, and And a blower 37.

The housing 30a includes a buried portion 30b embedded in the cover 12 and an exposed portion 30c exposed outside the cover 12.

A lamp 31 is installed in a part 30b of the housing 30a, and a reflecting plate 33 reflecting light to irradiate light emitted from the lamp 31 toward the culture tank 10 around the lamp 31 is provided. Is installed. In addition, a transparent body 35 is installed under the portion 30b of the housing 30a to protect the lamp 31 and to transmit the light irradiated from the lamp 31 to the culture tank 10. The transparent body 35 may be made of, for example, glass made of a light transmissive material, tempered glass, or a resin having predetermined heat resistance.

On the other hand, the infrared ray blocking film 37 for blocking the infrared rays included in the light is attached to the transparent body (35).

Furthermore, an air suction hole 30d is formed in the exposed portion 30c of the housing 30a to allow external air to flow in one side thereof, and the opposite side sucks the external air through the air suction hole 30d and the housing 30a. The blower 37 for forcibly discharging to the outside via the inside is installed. The air cooling system of the lighting unit 30 is to prevent the heat generated by the lamp 31 from affecting the culture medium, as well as to prevent overheating of the lamp 31 exposed to high temperature for a long time during the culture.

A plurality of cooling units 50 are installed along the culture medium cooling passages 13 in the air cooling housing 14, and each of the cooling units 50 has a cooling rod 51, a spiral heat radiation fin 53, and a sealing nut 55. It includes.

Referring to Figure 5, the cooling rod 51 is made of a metal material with excellent heat transfer, the lower end penetrates the culture medium cooling passage through the socket 52 installed in the culture medium cooling passage 13 so as to be in direct contact with the culture medium. 13) is purchased and installed.

The helical radiating fins 53 are coupled in a spiral direction along the cooling rods 51 to widen the area where the cooling unit 50 contacts the cooling air. In addition, the spiral heat sink fin 53 is a relatively high temperature compared to the upper end of the cooling rod 51 while the cooling air passing through the air cooling housing 14 is rotated from the bottom of the cooling rod 51 to the top along the spiral heat radiation fin (53). By transmitting the temperature of the lower portion of the cooling rod 51 to the upper side of the cooling rod 51, the cooling performance of the cooling unit 50 as a whole can be improved.

The sealing nut 55 is screwed to the cooling rod 51 to rotate along the cooling rod 51 to be fastened to the socket 52, thereby not only fixing the cooling rod 51 to the culture medium cooling passage 13. The airtightness of the insertion hole 13d formed for inserting the cooling rod 51 can be maintained.

The stirring unit 70 includes a motor 71, first and second magnetic driving units 73 and 75, a support shaft 77, a hollow shaft 78, and a plurality of impeller blades 79. The stirring unit 70 performs stirring in a manner of forced circulation of the culture medium, unlike the natural circulation method using the circulation pump of the conventional microbial incubator for effective agitation of the culture medium and microorganisms introduced into the culture tank 10. The hollow shaft 78 and the plurality of impeller blades 79 form an impeller.

In addition, the stirring unit 70 is preferably disposed so as to be eccentric from the center of the lower portion of the culture tank 10 and at an inclined angle so that the upper end is located in the center of the culture tank 10. That is, the stirring unit 70 is a drain for the collection of the culture medium is completed is installed in the center of the bottom of the culture tank 10, the hollow shaft 78 of the impeller is biased from the center of the bottom of the culture tank 10 May be placed in position.

In addition, the stirring unit 70 is the hollow shaft 78 of the impeller instead of the embodiment structure is installed in the center of the lower center of the culture tank 10, the drain for the collection of the culture medium is completed, the culture tank 10 It may be arranged in a position biased from the center of the bottom.

The motor 71 is disposed in the installation space 2a of the body 2 to transfer the rotational force to the first magnetic drive unit 73.

The first magnetic drive unit 73 has an annular rotating body 73a made of nonmagnetic material having corrosion resistance such as stainless steel and aluminum, and the rotating body 73a is mounted on a fixed bracket 72 provided on the body 2. And the center thereof is connected to the drive shaft 71a of the motor 71. In addition, as shown in FIG. 7, a plurality of permanent magnets 73b in the circumferential direction around the drive shaft 71a are formed on one surface of the rotating body 73a facing the second magnetic driving unit 75, for example, 12 permanent magnets. N poles and S poles are alternately embedded at 73 b at equal intervals. Furthermore, as shown in FIG. 8, the plurality of permanent magnets 73b are stably mounted to the rotating body 75a through the plastic cover 73c.

Like the first magnetic drive unit 73, the second magnetic drive unit 75 is made of a nonmagnetic material and has a rotating body 75a rotatably installed under the culture tank 10, and the first magnetic drive unit 73 and On the opposite surface, a plurality of permanent magnets 75b are arranged at equal intervals in the circumferential direction with respect to the center. In this case, the plurality of permanent magnets 75b are alternately arranged with the N poles and the S poles similarly to the first magnetic drive portion 73, and are mounted to the rotor 75a by the plastic lid 75c.

One end of the support shaft 77 is fixed to the bottom surface of the culture vessel 10, and the other end thereof is disposed at a predetermined height inside the culture vessel 10 and coaxially with the drive shaft 71a of the motor 71.

The plurality of impeller blades 79 are disposed at predetermined intervals up and down on the hollow shaft 78 into which the support shaft 77 is slidably inserted. Such a plurality of impellers 79 can form a plurality of holes (79a) to help the culture to break the particle size of oxygen supplied from the lower side through the oxygen supply nozzle (6) finely mixed in the culture medium. have. In this case, the impeller blades 79 may be inclined selectively for vortex generation.

It describes the action of the microorganism incubator 1 according to an embodiment of the present invention configured as described above.

First, after the culture medium consisting of water and the medium is introduced into the culture tank 10, the siege heater 16 is operated to heat the culture medium to a high temperature of about 95 ℃ for sterilization of the culture medium before the culture. If sterilization is done, the culture medium is cooled to a temperature suitable for incubation.

Subsequently, after the microbial strain is injected through the microbial strain inlet 9b, the medium inlet 9a and the microbial strain inlet 9b are closed in a sealed state and cultured.

Hereinafter, the cooling and stirring operations of the cultured solution required during the sterilization and culture of the cultured solution will be described in detail.

First, the cooling unit 50 and the stirring unit 70 are operated to cool and stir the sterilized culture medium.

When the stirring unit 70 is turned on, the first magnetic drive unit 73 connected to the drive shaft 71a rotates while the geared motor 71 is driven. Accordingly, while the attraction force is generated between the plurality of permanent magnets 73a of the first magnetic drive unit 73 and the plurality of permanent magnets 75a of the second magnetic drive unit 75 corresponding thereto, the second magnetic drive unit ( 75) rotates.

The second magnetic drive unit 75 transmits the rotational force to the hollow shaft 78 to rotate the plurality of impeller blades 79, thereby forcibly stirring the culture medium and microorganisms introduced into the space portion 10a of the culture tank 10. As a result, the culture conditions of the microorganisms can be provided as compared with the conventional natural circulation.

On the other hand, the cultured liquid forcibly circulated in the culture tank 10 by the stirring unit 70 is circulated in the space (10a) while a part is introduced into the cooling passage (13). Subsequently, the cultured liquid contacts the plurality of cooling rods 51 protruding into the cooling passage 13 while passing through the cooling passage 13.

The plurality of cooling rods 51 absorb the heat transferred from the hot culture medium, and the heat absorbed in this way is transferred to the spiral heat sink fins 53. In this case, the air passing through the air-cooled housing 14 moves along the helical heat dissipation fins 53 to the upper side of the plurality of cooling rods 51, and thus the plurality of cooling rods 51 are cooled.

As a result, while the heat exchange is continuously made through a plurality of cooling units 50, the temperature of the culture medium is gradually reduced. Accordingly, the culture medium at which the temperature is lowered is introduced into the culture tank 10 at a high speed through the outlet 13b of the cooling passage 13, which is gradually narrowed, and forms a vortex, and at the same time, the turning force by the guide plate 13c. This provision promotes circulation of the cultured liquid.

On the other hand, the microorganism incubator 1 of the present invention continuously detects the culture medium temperature in the culture tank 10 through the temperature sensor 16a, the low temperature of the outside air or a plurality of cooling unit 50 of the culture medium When the temperature is sensed below the microorganism culture temperature, it is possible to operate the siege heater 16 to maintain the temperature of the culture medium to the optimum temperature for culturing the microorganism.

The microorganisms are cultured through the light irradiated from the plurality of lighting units 30 during the predetermined culture period. On the other hand, when the microorganism is an aerobic microorganism such as yeast, bubbles are generated and the culture medium overflows above the proper level. In this case, in order to discharge the overflowed culture medium to the outside of the culture tank 10, the opening and closing valve 15a opens the overflow outlet 10c of the culture tank 10, and then the discharge of the culture medium is completed and the spring ( The overflow outlet 10c is closed again by 15b). By selectively opening and closing the overflow outlet 10c only when the overflowed culture medium is discharged, various bacteria existing in the outside air are blocked from being introduced into the culture tank 10 through the overflow outlet 10c, thereby preventing the microorganisms from becoming bacteria. To prevent contamination.

When the culture of the microorganisms is completed, the drain pipe 20 is opened to discharge the cultured microorganisms to the outside of the culture tank 10. Then, when cleaning the culture tank 10, the cover 9 can be opened and the upper part of the culture tank 10 can be opened for easy cleaning.

As described above, the microbial incubator 1 according to the exemplary embodiment of the present invention uses a magnetic coupling type stirring unit 70 to stir the culture medium, and a driving device installed outside the lower end of the culture tank 10 is cultured. The impeller can be rotated by rotating the magnetic drive unit installed in the inner side by the magnetic coupling method, so that the impeller is rotated in a state of completely sealing the culture tank 10, and thus, the conventional method for the transmission of the rotational power is performed. It does not require complex sealing structures such as

In addition, by installing the stirring unit 70 at the lower end of the culture tank 10, it becomes possible to easily install the lighting unit 30 on the cover of the culture tank top, even when cleaning the culture tank 10 Only the cover can be opened and cleaned without disassembling the motor.

As the plurality of lighting units 30 are installed on the upper side of the culture tank 10 with the upper portion open, light is irradiated into the culture tank 10 from the upper side of the culture tank 10 so that it is uniform to the microorganisms during the microbial culture time. It is possible to continuously irradiate a quantity of light to prevent the microbial culture rate is reduced.

In addition, the present invention is the culture tank 10 is made of a metal material, the sterilization by heating the culture medium through the sieve heater 16 to sterilize the culture medium by itself microorganism incubator 1 itself without the need to provide a separate sterilization device Can be carried out.

In addition, the present invention can cool the culture medium at a high speed through the plurality of cooling units 50, as well as increase the temperature of the culture medium through the siege heater 16 when the external temperature is low. Accordingly, it is possible to provide an optimal environment for culturing microorganisms by actively controlling the temperature regardless of the internal / external temperature, thereby maintaining a proper temperature suitable for culturing microorganisms at all times.

In addition, the present invention by the forced circulation of the culture medium using a plurality of impeller blades 79 of the stirring unit 70, the culture medium and the microorganisms can be uniformly stirred in all zones inside the culture tank (10). In addition, such a forced circulation system can also smoothly flow into and out of the cooling passage 13, which also helps the cooling of the cultured liquid.

The present invention by placing a stirring unit for the culture medium to the lower side of the culture tank to install a lighting unit for irradiating the light to the culture medium on the cover disposed on the upper side of the culture tank to perform light irradiation to the microorganisms efficiently The lowering of the culture rate can be prevented, and can be applied to a microorganism incubator using the lighting unit which can easily perform high temperature sterilization for sterilization of the culture medium using a metal culture tank.

1 is a front view showing a microbial incubator according to an embodiment of the present invention,

2 is a plan view seen from the direction II shown in FIG.

3 is a side cross-sectional view taken along line III-III shown in FIG. 2;

4 is an enlarged cross-sectional view showing a portion IV shown in FIG.

5 is an enlarged cross-sectional view showing part V shown in FIG. 1;

6 is an enlarged cross-sectional view showing a lighting unit;

FIG. 7 is an enlarged cross-sectional view illustrating a portion VII shown in FIG. 3,

8 is a view showing an arrangement structure of the magnetic disposed on the magnetic coupler,

FIG. 9 is an enlarged cross-sectional view illustrating a portion V shown in FIG. 3.

* Description of Signs for Main Parts in Drawings *

2: body 5: oxygen supply

6: oxygen supply nozzle 9: cover

10: culture tank 13: cooling passage

14: air-cooled housing 15: overflow pipe

15a: On-off valve 16: Sheath heater

20: drain pipe 30: lighting unit

50: cooling unit 51: cooling rod

53: spiral heat sink fin 70: stirring unit

Claims (7)

In the microbial incubator for culturing microorganisms by irradiating light through the lighting unit to the microorganisms introduced into the culture tank, The lighting unit A lamp for irradiating light is installed at a part embedded in a cover for opening and closing the upper part of the culture tank, and the air suction hole and the air suction hole through which external air flows into the lighting unit are provided in the remaining part exposed to the outside of the cover. And a blower for forcibly discharging the air introduced into the lighting unit to the outside of the lighting unit. In the microbial incubator for culturing microorganisms by irradiating light through the lighting unit to the microorganisms introduced into the culture tank, The lighting unit And a buried portion embedded inside the cover for opening and closing the upper portion of the culture tank and an exposed portion exposed to the outside of the cover, and the air is discharged at an opposite position between the air suction hole and the air suction hole where external air is sucked into the exposed portion of the housing. A housing having a hole; A lamp installed in an embedding portion of the housing; A reflection plate installed around the lamp to reflect light into the culture tank; A transparent body installed below the embedding part of the housing to transmit light emitted from the lamp; An infrared ray blocking film attached to the transparent body to block infrared rays included in light passing through the transparent body; And, And a blower installed in the air discharge hole to force the external air into and out of the housing. The method according to claim 1 or 2, The microbial incubator includes an impeller for stirring the culture medium; And The microorganism incubator is installed on the lower side of the culture tank further comprises a driving device for rotating the impeller. The microorganism incubator according to claim 1 or 2, wherein the culture tank is made of metal. The microorganism incubator according to claim 1 or 2, wherein the culture tank comprises a sieve heater for sterilization by heating the culture medium introduced into the culture tank to a high temperature. The method according to claim 1 or 2, At least one cooling unit for partially cooling the cultured liquid by heat exchange when the cultured liquid is circulated in one direction along the cooling passage formed at one side of the culture tank; And A stirring unit having an impeller rotatably installed on a support shaft installed at a lower end of the culture tank, the stirring unit being installed in the culture tank to circulate the cultured liquid and the microbial strain and the cultured liquid to the cooling passage; Microbial incubator. In the lighting unit for a microorganism incubator comprising a cover for opening and closing the culture tank and the upper side of the open tank, And a buried portion embedded inside the cover for opening and closing the upper portion of the culture tank and an exposed portion exposed to the outside of the cover, and the air is discharged at an opposite position between the air suction hole and the air suction hole where external air is sucked into the exposed portion of the housing. A housing having a hole; A lamp installed in an embedding portion of the housing; A reflection plate installed around the lamp to reflect light into the culture tank; A transparent body installed below the embedded portion of the housing to transmit light emitted from the lamp; An infrared ray blocking film attached to the transparent body to block infrared rays included in light passing through the transparent body; And, And a blower installed in the air discharge hole in order to forcibly flow in and out the outside air into the housing.

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