KR102130680B1 - Magnetic a culture medium - Google Patents

Abstract

The present invention relates to a magnetic incubator. More specifically, the present invention relates to the magnetic incubator capable of stably culturing large amounts of culture solutions inoculated with mushroom seedings through agitation in a sterile state, and when stirring, is completely blocked from the outside to prevent contamination. The magnetic incubator includes a chamber (10) and a stirring means (20).

Description

Magnetic a culture medium

The present invention relates to a magnetic incubator, and more particularly, to a culture medium stably cultivated in a sterile state through agitation in a sterile state, and more specifically, to a magnetic incubator capable of preventing contamination by completely blocking the outside when stirred. .

In general, mushrooms are microorganisms belonging to the fungi, and most of the mushrooms of various kinds are used for food, except for toxicity.

In order to cultivate these various types of mushrooms, the original bacteria of each mushroom are collected and cultivated in large quantities, and then the cultured bacteria are supplied to the medium to collect mushrooms from ordinary farms according to the cultivation environment of each mushroom.

In addition, as a general method of collecting and culturing protozoa, it can be roughly classified into a solid seed culture method and a liquid seed culture method.

This solid seed culture method uses sawdust as the main raw material, and sterilizes and crushes the auxiliary material, and then inoculates the original bacteria. However, it is difficult to manage the materials such as sawdust, and other bacteria enter the outside during cultivation, and the seed bacteria are contaminated. Along with the problem, there is a problem that mycelium is damaged by the main raw material during culture.

In addition, the liquid seed culture method has been proposed to solve the problems arising from the solid seed culture method, and requires a high level of technology to culture the seed.

Therefore, the liquid seed culture method maintains a sterile state inside the chamber, and after injecting the original bacteria and the culture solution, the seed bacteria are cultivated through stirring to prevent contamination by external germs and to prevent damage to the hyphae.

In order to be able to use such a liquid seed culture method, a "expansion culture medium for a seed mushroom culture solution" has been proposed.

In the prior art, after injecting the probiotic and the culture solution into the main body to maintain the sterile state, the culture is performed by stirring through the bubble action generated by air through an air supply device disposed outside.

However, the prior art is agitated by the bubbling action generated through air, so that the inside of the main body cannot be stirred evenly, and there is a problem in that seed germs may aggregate or deformation may occur in a specific part.

In addition, it is difficult to maintain complete sterility because power is supplied from the outside to agitate in the chamber for culturing liquid seed. It is difficult to maintain and maintain various germs and foreign substances due to aging, damage, etc. Is needed.

In addition, there is a problem in that miscellaneous bacteria can be mixed while moving the cultured seed germs.

Lastly, when the chamber is used for a long time, there is a problem in that facilities must be maintained to maintain cleanliness at all times due to the transformation of the protozoa and cultured spawns injected inside.

Korean Registered Patent No. 10-1473455 (Dec. 10, 2014)

The present invention has been devised to solve the above problems, and an object of the present invention is to provide a magnetic incubator capable of preparing a culture medium by mixing with a mushroom seed and an aqueous solution in a sterile state.

Another object of the present invention is to provide a magnetic incubator capable of preventing infestation of bacteria from the outside by stirring the culture medium in a space isolated from the outside because the stirring means is not exposed to the outside.

Another object of the present invention is to provide a magnetic incubator that stirs the culture medium using magnetic magnetic force, and stirs the culture medium evenly during stirring to uniformly stir.

Still another object of the present invention is to provide a magnetic incubator capable of preventing agglomeration of seed bacteria in a culture medium circulating along the up/down flow.

The present invention for achieving the above object is to accommodate the seed and aqueous solution, the chamber is formed on the first magnetic portion disposed on the lower surface; It is arranged inside the chamber, it characterized in that it is formed of a second magnetic portion corresponding to the first magnetic portion is formed of a stirring means rotating by magnetic force.

It is preferable that the first line for supplying the seed and aqueous solution to the chamber, for discharging the cultured medium, and the second line for supplying and discharging the inert gas, respectively.

The stirring means is disposed on the lower end of the chamber, the rotating plate is provided with a second magnetic portion, fixed to the rotating plate, the rotating shaft disposed in the vertical direction of the chamber, and fixed to the rotating shaft to be located on top of the rotating plate , It is preferably made of a first stirring blade formed of a mesh network, and fixed to the rotating shaft so as to be located on top of the first stirring blade, and a second stirring blade formed of a mesh network.

The first magnetic portion has a constant spacing of the lower surface of the chamber, a plurality of magnetic members are disposed in a circular shape, the second magnetic portion has a constant spacing inside the rotating plate, and a smaller diameter than the first magnetic portion It is preferable that a plurality of magnetic members are arranged in a circular shape to have.

The first stirring blade is spaced apart from the rotating shaft by a predetermined distance so that a first connection member is coupled so that the central portion is opened, and the second stirring blade is spaced apart from the rotating shaft by a predetermined distance wider than the spacing between the first stirring blades. It is preferable that the second connecting member is coupled so that the central portion is opened.

The rotating plate is coupled to the upper surface of the lower plate through a plurality of insertion protrusions coupled to the insertion hole on the lower surface and a lower plate formed with a plurality of insertion holes into which the second magnetic portion of the chamber is inserted. It is preferably made of a top plate on which three stirring blades are formed.

According to the magnetic incubator according to the present invention, after maintaining the interior of the chamber in a sterile state, there is an effect that can be prepared by mixing the mushroom seed and aqueous solution and culturing the seed strain.

According to the present invention, the stirring means can be rotated in a state that is not exposed to the outside, so that the culture medium is stirred while the chamber is completely isolated from the outside to prevent the introduction of various bacteria and the like.

According to the present invention, the culture medium is stirred by rotating the stirring means using the magnetic force of the magnetic, and when the medium is stirred, a flow in which the culture medium rises and falls is generated, so that the culture medium inside the chamber can be evenly stirred, so that it does not move to a corner or a specific part. It prevents the strains from being deformed, and has the advantage of evenly culturing the seed cells in the culture medium.

According to the present invention, there is an effect that can prevent agglomeration of the seed bacteria in the culture medium circulated along the upper / lower flow while passing through the mesh network.

1 is a perspective view showing a magnetic incubator according to the present invention,
Figure 2 is a cross-sectional view showing the internal configuration according to the invention,
Figure 3 is a perspective view showing a stirring means according to the invention,
4 is a conceptual view showing a rotating plate according to the present invention,
5 is a conceptual diagram showing first and second magnetic parts according to the present invention;
6 is an operation diagram showing an operation state according to the present invention.

Hereinafter, a magnetic incubator according to the present invention will be described in detail together with the accompanying drawings.

1 is a perspective view showing a magnetic incubator according to the present invention, Figure 2 is a cross-sectional view showing the internal configuration according to the present invention, Figure 3 is a perspective view showing a stirring means according to the present invention, Figure 4 is the present invention It is a conceptual diagram showing a rotating plate according to, Figure 5 is a conceptual diagram showing the first and second magnetic portions according to the present invention, Figure 6 is an operating diagram showing an operating state according to the present invention.

As shown in Figures 1 to 6, the present invention relates to a magnetic incubator, and more specifically, to stably culture a large amount through agitation in a sterile state of a culture medium inoculated with a mushroom spawn, and is completely blocked from contamination when stirred It relates to a magnetic incubator that can prevent.

To this end, the present invention is composed of a chamber 10 and a stirring means 20 so as to be able to be cultured through contaminating stirring after supplying the mushroom spawn and the culture solution.

The chamber 10 accommodates seed and aqueous solution, and a first magnetic portion 11 disposed on the lower surface is formed.

The stirring means 20 is disposed inside the chamber 10, and a second magnetic part 21 corresponding to the first magnetic part 11 is formed to rotate by magnetic force.

Here, the stirring means 20 is provided inside the chamber 10 so that it is not exposed to the outside.

As described above, the chamber 10 is stirred by the stirring means 20 while receiving the seed and aqueous solution to prepare a culture solution in which the seed is cultured.

Therefore, the chamber 10 and the stirring means 20 will be described in detail.

First, the chamber 10 maintains a sterile state, and maintains a sealed state to accommodate the supplied seed and aqueous solution.

Therefore, the chamber 10 is supplied with a seed and an aqueous solution, the first line 12 for discharging the cultured culture medium, and the second line 13 for supplying and discharging an inert gas are respectively coupled.

The first line 12 is formed of a first supply pipe (12a) for supplying seed and aqueous solution and a first discharge pipe (12b) for discharging the cultured culture.

In addition, the second line 13 is formed of a second supply pipe 13a for supplying a fluorinated gas and a second discharge pipe 13b for discharging a fluorinated gas supplied to the chamber 10.

Here, the first line 12 and the second line 13 are selectively detachably coupled to the chamber 10 through a connector structure.

Therefore, the first line 12 may supply the seed culture and the culture medium through the first supply tube 12a to supply the stirred culture medium to a separate culture tank or medium through the first discharge tube 12b.

In addition, the second line 13 supplies a fluorinated gas capable of maintaining an aseptic condition inside the chamber through the second supply pipe 13a, and through the second discharge pipe 13b when supplying seed and culture medium Discharge the supplied fluorinated gas.

Here, the fluorinated gas is supplied to the chamber 10 using various gases generally known as nitrogen, carbon dioxide, etc., to maintain sterile conditions.

Through this, the chamber 10 maintains an aseptic condition inside through a fluorinated gas, and can prevent contamination by various bacteria when culturing mushroom spawn by supplying spawn and aqueous solution.

In addition, the chamber 10 is sterilized and sterilized inside the chamber after discharging seed and culture liquid by supplying high-temperature steam through the second line 13.

In addition, the chamber 10 is provided with a separate cover, the cover can be opened and closed, it is preferable to be configured to maintain a sterile state by sealing the inside of the chamber during cultivation, and wash the interior after the culture is over Do.

The agitation means 20 agitates the culture solution inside the chamber 10 to facilitate cultivation of seed germs and prevents agglomeration or deformation.

The rotating plate 22 is disposed at the bottom of the chamber 10, and a second magnetic portion 21 is installed.

The stirring means 20 is composed of a rotating plate 22, a rotating shaft 25, a first stirring wing 26, and a second stirring wing 27.

The rotating shaft 25 is fixed to the rotating plate 22 and is disposed in the vertical direction of the chamber 10.

At this time, the rotating shaft 25 penetrates the rotating plate 22 so that the lower end is rotatably supported by the chamber 10.

In addition, it is preferable that a support groove 14 for rotatably supporting the end of the rotating shaft 25 is inserted at a central lower end of the chamber 10.

Therefore, the rotating shaft 25 is supported by the rotating plate 22 and the supporting groove 14 to stably rotate.

The first stirring blade 26 is fixed to the rotating shaft 25 so as to be located on the upper end of the rotating plate 22, it is formed of a mesh network.

Therefore, the first stir blade 26 is spaced apart at regular intervals along the circumference of the rotating shaft 25 so that the first connection member 26a is coupled to open the central portion.

The first connecting member 26a is formed such that one side is connected to the rotating shaft 25 and the other side supports the first stirring blade 26 to open the central portion.

And the mesh network of the first connecting member (26a) is made of a size of about 100mesh to prevent the seed germ in the chamber 10 is agglomerated, it is made not to interfere with the stirring flow of the culture medium.

The second stirring blade 27 is fixed to the rotating shaft 25 so as to be located on the upper end of the first stirring blade 26, it is formed of a mesh network.

The second stirring blade 27 is coupled to the second connecting member 27a so as to be spaced apart from the rotating shaft 25 at a predetermined interval wider than the spaced apart distance of the first stirring blade 26.

Accordingly, the first stirring blade 26 is connected to the same configuration, but the second connecting member 27a is formed to be relatively longer than the first connecting member 26a, and the open central portion has the first stirring wing ( 26).

In addition, the second stirring wing 27 is formed with a shorter length than the first stirring wing 26, the length of the first stirring wing 26 and the first connecting member (26a) and the second stirring wing The overall length of 27 and the second connecting member 27a is the same.

In addition, the mesh network of the second stirring wing 27 is formed to be about 50 mesh in size and is made more compact than the first stirring wing 26 to prevent seeding from agglomerating and not to interfere with the agitated flow of the culture medium.

At this time, a plurality of the first stirring blade 26 and the second stirring blade 27 are disposed along the circumference of the rotating shaft 25, but two or more are arranged for the size and stirring of the chamber 10 It is desirable to be.

Accordingly, the first magnetic portion 11 and the second magnetic portion 21 will be described in detail as follows.

First, the first magnetic portion 11 has a certain distance of the lower surface of the chamber 10, a plurality of magnetic members (mu) are arranged in a circular shape, the second magnetic portion 21 is the rotating plate ( 22), a plurality of magnetic members (mu) are arranged in a circular shape so as to have a smaller diameter than the first magnetic part (11).

Here, four or more magnetic members mu are installed according to the size of the chamber, and it is preferable that the first magnetic part 11 and the second magnetic part 21 have the same number.

That is, as shown in FIG. 5, the first magnetic part 11 has a radius of R1 from the center of the chamber 10 as a starting point, and the magnetic member mu is arranged to have a circular shape at regular intervals. do.

At this time, the first magnetic portion 11 is located in any one of the upper, lower, and inner lower surfaces of the chamber 10 and is made to transmit magnetic force.

And as shown in Figure 5, the second magnetic portion 21 has a radius of R2 having a diameter smaller than the radius of R1 from the center of the rotation as a starting point, and the magnetic member to have a circular shape at regular intervals ( mu) is placed.

Here, the second magnetic portion 21 is located inside the rotating plate 22 so as to receive the magnetic force of the first magnetic portion 11.

Therefore, the first magnetic portion 11 and the second magnetic portion 21 have a diameter of R2 smaller than R1, and R1=R2+R3 has the same diameter.

In addition, R1>R2 is arranged to have a radius, R3 is a portion of the magnetic member (mu) so that the magnetic member (mu) of the first magnetic portion (11) and the second magnetic portion (21) overlap a certain portion It has a size smaller than the diameter.

Through this, the magnetic force generated in the first magnetic portion 11 is transmitted to the second magnetic portion 21 to rotate the rotating plate 22.

In addition, the magnetic member (mu) of the first magnetic portion (11) has an S-pole at the top, and the magnetic member (mu) of the second magnetic portion (21) is arranged so that the lower end has an N-pole magnetic force It is preferably made to be rotated by.

In addition, the electrodes of the first magnetic portion 11 and the second magnetic portion may be formed in reverse.

In addition, the rotating plate 22 is provided with the second magnetic portion 21, and is composed of a lower plate 23 and an upper plate 24 so as to be rotatable inside the chamber 10.

The lower plate 23 is formed with a plurality of insertion holes 23a into which the second magnetic portion 21 of the chamber 10 is inserted.

The upper plate 24 is coupled to the upper surface of the lower plate 23 through a plurality of insertion protrusions 24a coupled to the insertion hole 23a on the lower surface, and a plurality of third stirrings on the upper plate 24 Wings 28 are formed.

Therefore, after inserting a plurality of the magnetic member (mu) into the insertion hole (23a) of the lower plate 23, the insertion protrusion (24a) of the upper plate 24 is inserted into the insertion hole (23a) to the magnetic The lower plate 23 and the upper plate 24 are fixed so that the member mu is not exposed to the outside.

And the third stirring blade 28 is arranged in a plurality at regular intervals on the top plate 24, when the rotating plate 22 rotates to generate a flow toward the top of the seed and aqueous solution stored in the chamber 10 upwards .

Accordingly, a plurality of the third stirring blades 28 are arranged from the center of the rotating plate 22 toward the outer surface at regular intervals along the circumference of the rotating plate 22, and protrude to the upper portion of the top plate 24.

Here, the third stirring blade 28 is formed with a curved surface 28a with one side protruding in accordance with the rotational direction of the rotating shaft 25 from bottom to top.

That is, the curved surface (28a) is made of a semi-cured, inclined, etc. to generate a flow of upward movement of the seed and aqueous solution stored in the chamber 10 when the rotating plate 22 is rotated.

In addition, a buried groove 15 in which the rotating plate 22 is rotatably inserted is formed on a lower surface of the chamber 10.

The buried groove 15 is recessed so that the upper end of the rotating plate 22 coincides with the lower surface of the chamber 10, but the rotating plate 22 is made rotatable.

At this time, the first magnetic portion 11 is located on the lower surface of the buried groove 15 to rotate the rotating plate 22 by magnetic force.

Next, as shown in Figure 6 will be described with respect to the operating state for culturing the seed.

First, after the agitation means 20 is coupled to the chamber 10, the first line 12 and the second line 13 are connected and installed.

And the stirring means 20 is not exposed to the outside is disposed in the chamber 10.

Thereafter, an inert gas is supplied through the second line 13 to maintain the interior of the chamber 10 aseptically.

In addition, seed and aqueous solutions are supplied through the first line 12.

When the seed and aqueous solution are stored in the chamber 10 in which the inside is maintained aseptically, the seed and aqueous solution are stirred through the stirring means 20 to cultivate the seed and prepare a culture solution.

Therefore, when power is supplied to the first magnetic part 11, magnetic force is generated from the magnetic member mu.

And the second magnetic portion 21 is moved by the magnetic force of the first magnetic portion 11 to rotate the rotating plate 22, it is possible to control the speed of 1 ~ 600rpm.

When the rotating plate 22 is rotated in this way, the culture solution stored in the chamber 10 is stirred by the third stirring blade 28.

Therefore, the culture medium is formed by a third stirring wing 28, a flow rising in the central portion of the chamber 10 is formed, and the central portion of the chamber 10 rises to move outward from the top of the chamber 10. Afterwards, a downward flow is formed in the outer portion of the chamber 10 to circulate the culture medium.

At this time, the first stirring wing 26 and the second stirring wing 27 prevents the seeding of the culture medium to rise or fall through the mesh network.

In addition, as the central portion of the first stirring wing 26 and the second stirring wing 27 is opened, a culture medium rises and flows from the central portion of the chamber 10 to the outer portion.

As described above, the whole of the culture solution inside the chamber 10 is stirred by the stirring means 20 along the flow of ascending and descending to facilitate the cultivation of seed germs. It is prevented from being deformed due to bunching or standing.

In addition, the stirring means 20 can be rotated through the power supply of the first magnetic part 11 and the second magnetic part 21 in a state in which the stirring means 20 is not exposed to the outside, so that the outside during the culture It blocks the influx of germs and is easy to maintain.

When the culture in the chamber 10 is completed in this way, it can be supplied to the outside through the first discharge tube 12b of the first line 12 to be supplied as a stirring tank or medium for secondary culture.

Here, since the culture medium is not exposed to the outside, it can be prevented from being contaminated by germs introduced from the outside.

In addition, it is possible to sterilize and disinfect the seed bacteria remaining in the chamber by supplying high-temperature steam through the second line 13 after the culture medium in the chamber 10 is completely discharged.

In addition, the chamber 10 may be opened to periodically clean by an administrator or operator to prevent contamination of the interior of the chamber 10.

As described above, the rights of the present invention are not limited to the above-described embodiments, but are defined by the claims, and various modifications and adaptations are possible within the scope of the rights described in the claims by those skilled in the art. It is obvious that you can do it.

10: chamber 11: first magnetic part 12: first line
12a: First supply pipe 12b: First discharge pipe
13: 2nd line 13a: 2nd supply pipe
13b: second discharge pipe 14: support groove
15: Landfill home
20: stirring means 21: second magnetic portion 22: rotating plate
23: lower plate 23a: insertion hole
24: top plate 24a: insertion projection
25: rotating shaft 26: first stirring wing
26a: first connecting member 27: second stirring wing
27a: 2nd connecting member 28: 3rd stirring blade
28a: curved surface
mu: No magnetic

Claims (6)

A chamber 10 accommodating seed bacteria and an aqueous solution and having a first magnetic portion 11 disposed on a lower surface;
Arranged inside the chamber 10, the second magnetic portion 21 corresponding to the first magnetic portion 11 is formed is made of a stirring means 20 that rotates by magnetic force,
The chamber 10 is supplied with a seed and an aqueous solution, the first line 12 for discharging the cultured culture medium, and the second line 13 for supplying and discharging an inert gas are respectively coupled,
The stirring means 20,
A rotating plate 22 disposed at a lower end of the chamber 10 and having the second magnetic portion 21 installed thereon,
A rotating shaft 25 fixed to the rotating plate 22 and disposed in a vertical direction of the chamber 10,
It is fixed to the rotating shaft 25 so as to be located at the top of the rotating plate 22, the first stirring blade 26 formed of a mesh network,
It is fixed to the rotating shaft 25 so as to be located on the upper end of the first stirring blade 26, made of a second stirring blade 27 formed of a mesh network,
The mesh mesh of the first stirring wing 26,
It is formed in a size of 100 mesh to prevent the seed bacteria in the chamber 10 from agglomeration
The mesh mesh of the second stirring wing 27,
50 mesh size is formed more densely than the mesh network of the first stirring blade 26 to prevent the seed germ in the chamber 10 from agglomerating,
The first stirring blade 26 is spaced a predetermined distance from the rotating shaft 25 is coupled to the first connecting member (26a) to open the central portion,
The second stirring wing 27 is spaced apart from the rotating shaft 25 by a predetermined interval wider than the spaced apart distance of the first stirring wing 26, the second connecting member 27a is coupled to open,
The rotating plate 22,
A lower plate 23 having a plurality of insertion holes 23a into which the second magnetic portion 21 of the chamber 10 is inserted,
It is coupled to the upper surface of the lower plate 23 through a plurality of insertion protrusions (24a) coupled to the insertion hole (23a) on the lower surface, the upper plate 24 is formed with a plurality of third stirring blades (28) on the upper surface Is made of,
The third stirring wing 28,
Magnetic incubator, characterized in that one side protruding in accordance with the rotational direction of the rotating shaft 25 is formed with a curved surface (28a) from the bottom to the top. delete delete According to claim 1,
The first magnetic portion 11 has a constant spacing of the lower surface of the chamber 10, a plurality of magnetic members (mu) are arranged in a circle,
The second magnetic portion 21 has a certain interval in the interior of the rotating plate 22, characterized in that a plurality of magnetic members (mu) are arranged in a circular shape to have a smaller diameter than the first magnetic portion (11) Magnetic incubator.
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