CN114540190A - Poison seed suspension incubator - Google Patents
Poison seed suspension incubator Download PDFInfo
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- CN114540190A CN114540190A CN202210138604.7A CN202210138604A CN114540190A CN 114540190 A CN114540190 A CN 114540190A CN 202210138604 A CN202210138604 A CN 202210138604A CN 114540190 A CN114540190 A CN 114540190A
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- 239000000725 suspension Substances 0.000 title claims abstract description 23
- 239000002574 poison Substances 0.000 title abstract description 10
- 231100000614 poison Toxicity 0.000 title abstract description 10
- 241000700605 Viruses Species 0.000 claims abstract description 58
- 238000004114 suspension culture Methods 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims description 22
- 230000005540 biological transmission Effects 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 15
- 238000006073 displacement reaction Methods 0.000 claims description 13
- 238000004804 winding Methods 0.000 claims description 13
- 229910001220 stainless steel Inorganic materials 0.000 claims description 10
- 239000010935 stainless steel Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 229920005830 Polyurethane Foam Polymers 0.000 claims description 4
- 239000011496 polyurethane foam Substances 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 229910001410 inorganic ion Inorganic materials 0.000 claims description 3
- 150000002772 monosaccharides Chemical class 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 238000011218 seed culture Methods 0.000 abstract description 19
- 238000003756 stirring Methods 0.000 abstract description 9
- 230000002688 persistence Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 18
- 238000000034 method Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 11
- 230000007717 exclusion Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000005096 rolling process Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000005188 flotation Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a virus seed suspension culture box, relates to the technical field of virus seed culture, and aims to solve the problems that most of the existing virus seed suspension culture adopts stirring and air floatation modes to suspend cells hosted by virus seeds in a culture solution, the suspension persistence of the modes is insufficient, and the stable culture environment of the virus seeds can be damaged during stirring and air floatation operations. The lower extreme of push rod control cabinet is provided with horizontal platform seat, the upper end of horizontal platform seat is provided with two electric putter, the upper end of push rod control cabinet is provided with the incubator, the inside of incubator is provided with cultivates the internal frame, the inside of cultivateing the internal frame is provided with cultivates the groove between, the inside in groove is provided with between groove supporting seat between cultivateing, the inside of groove supporting seat is provided with the culture tank and puts the groove between, the upper end that the groove was put to the culture tank is provided with transparent culture tank, the inside of transparent culture tank is provided with the charge disc, the top of disc is provided with the poison kind of charge culture solution.
Description
Technical Field
The invention relates to the technical field of virus seed culture, in particular to a virus seed suspension incubator.
Background
The virus needs to be cultured when the virus is subjected to experiments, the virus seeds are cultured under the laboratory conditions and are protoplasts of the virus, the suspension culture method is mostly adopted for culturing the virus seeds in the culture process, the suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells, certain anchorage-dependent cells can also be cultured by the method after adaptation and selection, the increase of the suspension culture scale is relatively simple, the depth is more than 5mm, the culture medium needs to be stirred and is more than 10cm, and CO2 and air need to be deeply introduced to ensure sufficient gas exchange. The cells are always in a dispersed suspension in the culture solution by shaking or rotating the device.
Most of the existing virus seed suspension culture methods adopt stirring and air floatation methods to enable cells hosted by virus seeds to be suspended in a culture solution, the suspension persistence of the methods is insufficient, and the stable culture environment of the virus seeds can be damaged during stirring and air floatation operations, so that the survival rate of the virus seed culture is influenced, and therefore, a virus seed suspension culture box is provided.
Disclosure of Invention
The invention aims to provide a virus seed suspension culture box, which aims to solve the problems that most of the conventional virus seed suspension culture methods proposed in the background art adopt stirring and air floatation methods to suspend cells hosted by virus seeds in a culture solution, the suspension persistence of the methods is insufficient, and the stable culture environment of the virus seeds is damaged during stirring and air floatation operations.
In order to achieve the purpose, the invention provides the following technical scheme: a suspension incubator for seeds of poison comprises a push rod control cabinet, wherein a horizontal platform seat is arranged at the lower end of the push rod control cabinet, two electric push rods are arranged at the upper end of the horizontal platform seat, an incubator is arranged at the upper end of the push rod control cabinet, an inner culture frame is arranged in the incubator, an intermediate culture tank is arranged in the inner culture frame, an intermediate tank support seat is arranged in the intermediate culture tank, an intermediate culture tank placing tank is arranged in the intermediate tank support seat, a transparent culture tank is arranged at the upper end of the intermediate culture tank placing tank, a charged disc is arranged in the transparent culture tank, a seed culture solution is arranged above the charged disc, the seed culture solution is a mixed aqueous solution of pure water, amino acid, monosaccharide, vitamin, inorganic ions and trace elements, and a rear connecting plate is arranged on the rear end face of the intermediate culture tank, the front end face of the rear connecting plate is provided with a wireless network camera, and the front end face of the incubator is provided with a movable door sealing plate.
Preferably, be provided with the sealing groove on the position that dodge gate shrouding and incubator are connected, the sealing groove sets up with incubator integrated into one piece, all be provided with the direction logical groove on the both sides inner wall in sealing groove, the direction leads to the groove and sets up with sealing groove integrated into one piece, the inside that the direction led to the groove is provided with the transmission armed lever, the both ends of transmission armed lever respectively with dodge gate shrouding and electric putter's output welded connection.
Preferably, the inner walls of the two sides of the guide through groove are provided with through groove inner walls, the through groove inner walls and the guide through groove are integrally formed, the outer walls of the two sides of the transmission arm rod are provided with arm rod inner grooves, the arm rod inner grooves are internally provided with auxiliary rollers, and the transmission arm rod is in rolling connection with the through groove inner walls through the auxiliary rollers.
Preferably, the inner walls of the two sides of the arm lever inner groove are provided with bearings, the bearings are fixedly connected with the arm lever inner groove through screws, the outer walls of the two sides of the auxiliary roller are provided with connecting shafts, the connecting shafts and the auxiliary roller are integrally formed, and the auxiliary roller is movably connected with the bearings through the connecting shafts on the outer walls of the two sides.
Preferably, be provided with the internal frame back plate on the rear end position of training the internal frame, be provided with four corresponding connecting rods on the rear end inner wall of incubator, the both ends that correspond the connecting rod respectively with incubator and internal frame back plate welded connection, the top of incubator is provided with the power supply cabinet, the inside of internal frame back plate is provided with twelve winding displacement perforations, and the winding displacement perforation sets up with internal frame back plate integrated into one piece, twelve winding displacement perforations respectively with twelve cultivate the intervallum groove one-to-one, the electricity of wireless network camera is served and is provided with the connecting wire.
Preferably, four be provided with integrated row of inserting on the position between the corresponding connecting rod, integrated row of inserting passes through screw and incubator fixed connection, twelve connect the electric wire and run through the winding displacement perforation and with integrated row electric connection that inserts, integrated row of inserting connects to be provided with the power supply wiring on holding, integrated row of inserting passes through power supply wiring and power supply cabinet electric connection.
Preferably, the incubator is made of stainless steel, the thickness of the stainless steel is twenty centimeters, and the inner wall of the stainless steel is lined with ten centimeters of polyurethane foam.
Preferably, a manual operator is arranged on the front end face of the push rod control cabinet, the output end of the manual operator is electrically connected with the input end of the push rod control cabinet, and the output end of the push rod control cabinet is electrically connected with the input ends of the two electric push rods.
Preferably, be provided with wireless AP, router and network video recorder on the rear end outer wall of incubator, be provided with supporting platform on one side outer wall of incubator, supporting platform and incubator integrated into one piece set up, supporting platform's upper end is provided with the display, wireless network camera's output and wireless AP's input transmission connection.
Preferably, the output end of the wireless AP is electrically connected with the input end of the router, the output end of the router is electrically connected with the input end of the network video recorder, and the output end of the network video recorder is electrically connected with the input end of the display.
Compared with the prior art, the invention has the beneficial effects that:
1. the suspension culture box of the invention adopts the principle of mutual exclusion of same level of charges to realize the effect of suspension culture of the virus seeds without stirring and floating operation, the main body of the virus seed culture solution is pure water which is not conductive, a disc with charges of same level is arranged below the virus seed culture solution, the virus seeds are lodged and cultured in cells which are positioned inside the virus seed culture solution, according to the principle of mutual exclusion of the same level of charges, the cells in the virus seed culture solution are suspended in the virus seed culture solution under the influence of charge repulsion, because the pure water is not conductive, the suspension process can not affect the culture environment of the virus seeds, overcomes the defect that the prior suspension culture of the virus seeds mostly adopts stirring and air flotation modes to suspend the cells hosted by the virus seeds in the culture solution, these methods have problems that the suspension is not sufficiently durable and the stable culture environment of the seed is damaged during the agitation and air flotation operations.
2. The cultivation is not finished, the box does not need to be opened, the virus seed suspension cultivation condition in each cultivation chamber can be monitored in real time through the wireless network cameras, each cultivation chamber is provided with an independent wireless network camera, twelve wireless network cameras receive the power supply of the top power supply cabinet, the wireless network cameras upload the images monitored in real time to the display through the wireless AP, the router and the network hard disk video recorder to display, and therefore the virus seed suspension cultivation condition can be mastered in real time under the condition that the box does not need to be opened.
3. The chamber door of incubator is connected by hinge formula among the prior art and is changed into the gate-type, has improved closed sealed degree, has reduced the influence that external factor caused incubator internal environment, after the cultivation cycle, accessible presses two electric putter synchronous jack-ups of button control on the manual operator when needing to use the poison seed to open the dodge gate shrouding, be convenient for take out the poison seed of cultivation.
Drawings
FIG. 1 is a schematic view of the overall structure of the suspension incubator for virus seeds according to the present invention;
FIG. 2 is a schematic view of the overall closed structure of the suspension incubator for virus seeds according to the present invention;
FIG. 3 is a schematic view of the internal structure of a culture chamber of the present invention;
FIG. 4 is a schematic view of the construction of a transparent culture tank of the present invention;
FIG. 5 is a schematic diagram of the construction of the rear panel of the inner frame of the present invention;
FIG. 6 is a schematic view of a connecting structure of a driving arm lever and a guiding through groove of the present invention;
FIG. 7 is a schematic view of the wireless network camera working process of the present invention;
in the figure: 1. the push rod controls the chassis; 2. a horizontal platform base; 3. a manual operator; 4. an electric push rod; 5. an incubator; 6. culturing an inner frame; 7. a movable door closing plate; 8. sealing the connecting groove; 9. a guide through groove; 10. a drive arm; 11. a support platform; 12. a display; 13. a culture compartment; 14. a middle groove supporting seat; 15. a transparent culture tank; 16. a power supply cabinet; 17. a rear connecting plate; 18. a culture tank placing groove; 19. a wireless network camera; 20. connecting a wire; 21. a charged disc; 22. a seed culture solution; 23. an inner frame rear plate; 24. a corresponding connecting rod; 25. perforating the flat cable; 26. an integrated power strip; 27. a power supply wiring; 28. the inner wall of the through groove is provided with a groove; 29. an arm lever inner groove; 30. an auxiliary roller; 31. a bearing; 32. a connecting shaft; 33. a wireless AP; 34. a router; 35. network hard disk video recorder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-7, an embodiment of the present invention is shown: a suspension incubator for virus seeds comprises a push rod control case 1, a horizontal platform base 2 is arranged at the lower end of the push rod control case 1, two electric push rods 4 are arranged at the upper end of the horizontal platform base 2, an incubator 5 is arranged at the upper end of the push rod control case 1, an inner culture frame 6 is arranged inside the incubator 5, an incubation chamber groove 13 is arranged inside the inner culture frame 6, an chamber groove supporting base 14 is arranged inside the incubation chamber groove 13, an incubator placing groove 18 is arranged inside the chamber groove supporting base 14, a transparent incubator 15 is arranged at the upper end of the incubator placing groove 18, a disc 21 with charges is arranged inside the transparent incubator 15, a virus seed culture solution 22 is arranged above the disc 21 with charges, the virus seed culture solution 22 is a mixed aqueous solution of pure water, amino acid, monosaccharide, vitamin, inorganic ions and trace elements, a post-positioned connecting plate 17 is arranged on the rear end face of the incubation chamber groove 13, the front end face of the rear connecting plate 17 is provided with a wireless network camera 19, and the front end face of the incubator 5 is provided with a movable door closing plate 7.
Further, be provided with sealing connection groove 8 on the position that dodge gate shrouding 7 and incubator 5 are connected, sealing connection groove 8 sets up with incubator 5 integrated into one piece, all be provided with the logical groove 9 of direction on sealing connection groove 8's the both sides inner wall, the logical groove 9 of direction sets up with sealing connection groove 8 integrated into one piece, the inside that leads to groove 9 is provided with transmission armed lever 10, the both ends of transmission armed lever 10 respectively with dodge gate shrouding 7 and electric putter 4's output welded connection, sealing connection groove 8 that sets up on the position that dodge gate shrouding 7 and incubator 5 are connected plays the effect of being convenient for dodge gate shrouding 7 and incubator 5 activity sealing connection, the logical groove 9 of direction that all sets up on the both sides inner wall of sealing connection groove 8 plays the effect of being convenient for transmission armed lever 10 direction activity, transmission armed lever 10 that the inside that leads to groove 9 set up plays the effect of transmission electric putter 4 and dodge gate shrouding 7.
Further, the inner walls of the two sides of the guide through groove 9 are provided with through groove inner wall grooves 28, the through groove inner wall grooves 28 and the guide through groove 9 are formed integrally, the outer walls of the two sides of the drive arm 10 are provided with arm rod inner grooves 29, the inside of the arm rod inner grooves 29 is provided with auxiliary rollers 30, the drive arm 10 is in rolling connection with the through groove inner wall grooves 28 through the auxiliary rollers 30, the through groove inner wall grooves 28 arranged on the inner walls of the two sides of the guide through groove 9 play a role in facilitating the rolling contact connection of the auxiliary rollers 30 and the guide through groove 9, and the arm rod inner grooves 29 arranged on the outer walls of the two sides of the drive arm 10 play a role in bearing the auxiliary rollers 30.
Further, bearings 31 are arranged on inner walls of two sides of the arm lever inner groove 29, the bearings 31 are fixedly connected with the arm lever inner groove 29 through screws, connecting shafts 32 are arranged on outer walls of two sides of the auxiliary roller 30, the connecting shafts 32 and the auxiliary roller 30 are integrally formed, the auxiliary roller 30 is movably connected with the bearings 31 through the connecting shafts 32 on the outer walls of the two sides, the bearings 31 arranged on the inner walls of two sides of the arm lever inner groove 29 play a role in movably connecting the auxiliary connecting shafts 32, and the connecting shafts 32 arranged on the outer walls of two sides of the auxiliary roller 30 play a role in facilitating connection of the auxiliary roller 30 and the bearings 31.
Furthermore, an inner frame rear plate 23 is arranged at the rear end of the culture inner frame 6, four corresponding connecting rods 24 are arranged on the inner wall of the rear end of the incubator 5, the both ends that correspond connecting rod 24 respectively with incubator 5 and internal frame back plate 23 welded connection, the top of incubator 5 is provided with power supply cabinet 16, the inside of internal frame back plate 23 is provided with twelve winding displacement perforation 25, winding displacement perforation 25 sets up with internal frame back plate 23 integrated into one piece, twelve winding displacement perforation 25 respectively with twelve cultivate between groove 13 one-to-one, be provided with power connection wire 20 on wireless network camera 19's the power connection end, cultivate the internal frame back plate 23 that sets up on the rear end position of internal frame 6 and play the effect of bearing twelve winding displacement perforation 25, power supply cabinet 16 that the top of incubator 5 set up plays the effect for wireless network camera 19 power supply, the twelve winding displacement perforation 25 that the inside of internal frame back plate 23 set up play the effect that is convenient for power connection wire 20 to run through and arranges.
Furthermore, four positions between the corresponding connecting rods 24 are provided with integrated socket bars 26, the integrated socket bars 26 are fixedly connected with the incubator 5 through screws, twelve electric wires 20 penetrate through the flat cable through holes 25 and are electrically connected with the integrated socket bars 26, the electric connection ends of the integrated socket bars 26 are provided with power supply connection wires 27, the integrated socket bars 26 are electrically connected with the power supply cabinet 16 through the power supply connection wires 27, the integrated socket bars 26 arranged at the positions between the four corresponding connecting rods 24 play a role in conveniently and uniformly connecting the twelve electric wires 20, and the power supply connection wires 27 arranged at the electric connection ends of the integrated socket bars 26 play a role in conveniently connecting the integrated socket bars 26 with the power supply cabinet 16 for power supply.
Furthermore, the incubator 5 is made of stainless steel, the thickness of the stainless steel is twenty centimeters, the inner wall of the stainless steel is lined with ten centimeters of polyurethane foam, the heat insulation material of the incubator body is made of polyurethane foam, the incubator body has strong anti-interference capability on external cold and heat sources, and the incubator body is made of stainless steel and has good corrosion resistance.
Further, a manual operator 3 is arranged on the front end face of the push rod control case 1, the output end of the manual operator 3 is electrically connected with the input end of the push rod control case 1, the output end of the push rod control case 1 is electrically connected with the input ends of the two electric push rods 4, and the manual operator 3 arranged on the front end face of the push rod control case 1 plays a role of sending an instruction to the push rod control case 1, so that the purpose of controlling and adjusting the electric push rods 4 is achieved.
Further, be provided with wireless AP33 on the rear end outer wall of incubator 5, router 34 and network hard disk video recorder 35, be provided with supporting platform 11 on one side outer wall of incubator 5, supporting platform 11 sets up with incubator 5 integrated into one piece, supporting platform 11's upper end is provided with display 12, wireless network camera 19's output and wireless AP 33's input transmission connection, the wireless AP33 that sets up on the rear end outer wall of incubator 5, router 34 and network hard disk video recorder 35 play the effect of constituteing wireless image signal transmission system.
Further, the output end of the wireless AP33 is electrically connected to the input end of the router 34, the output end of the router 34 is electrically connected to the input end of the network hard disk recorder 35, and the output end of the network hard disk recorder 35 is electrically connected to the input end of the display 12.
The working principle is as follows: the suspension culture box adopts the principle of mutual exclusion of the same level of charges to realize the effect of suspension culture of the virus seeds without stirring and floating operation, firstly selects proper boarder cells according to the types of the virus seeds to be cultured, prepares a disc 21 with charges the same as the selected boarder cells according to the charges carried by the selected boarder cells, for example, the surface of a normal erythrocyte is negatively charged, the surface of a myocardial cell in a stationary period is positively charged, so the charges carried by the prepared disc 21 are determined by the charges carried by the cells to be cultured by the virus seeds, the preparation of the disc 21 adopts the reason of electrostatic induction, puts the disc 21 with charges into an electrostatic field, obtains the positive charges carried from a negative pole, obtains the negative charges carried from a positive pole, the main body of the virus seed culture solution 22 is pure water, the pure water is not conductive, the disc 21 with charges of the same level is put below the virus seed culture solution 22, the virus seeds are hosted and cultured in the cells, the cells are positioned in the virus seed culture solution 22, according to the principle of the mutual exclusion of the same level of charges, the cells in the virus seed culture solution are influenced by the mutual repulsion of the charges and are suspended in the virus seed culture solution, the suspension process cannot influence the virus seed culture environment because pure water is not conductive, the door of the incubator 5 is replaced by a shutter type door from hinge type connection in the prior art, the closed sealing degree is improved, the influence of external factors on the internal environment of the incubator 5 is reduced, the incubator does not need to be opened before the culture is not finished, the suspension culture condition of the virus seeds in each culture compartment groove 13 can be monitored in real time through the wireless network cameras 19, each culture compartment groove 13 is provided with an independent wireless network camera 19, twelve wireless network cameras 19 receive the power supply of the top power supply cabinet 16, and the wireless network cameras 19 monitor the suspension culture condition of the virus seeds in each culture compartment groove 13 through the wireless AP33, Router 34 and network video recorder 35 upload real time monitoring's image to display 12 on showing to also can master the circumstances that the suspension of poison kind was cultivateed in real time under the circumstances that need not to unpack, after the cultivation cycle, the accessible presses two electric putter 4 synchronous jack-ups of button control on the manual operator 3 when needing to use the poison kind, thereby open dodge gate shrouding 7, be convenient for take out the poison kind of cultivation, accomplish the use work of poison kind suspension incubator.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. The utility model provides a virus kind suspension incubator, includes push rod control machine case (1), its characterized in that: the lower extreme of push rod control machine case (1) is provided with horizontal platform seat (2), the upper end of horizontal platform seat (2) is provided with two electric putter (4), the upper end of push rod control machine case (1) is provided with incubator (5), the inside of incubator (5) is provided with cultivates internal frame (6), the inside of cultivateing internal frame (6) is provided with cultivates between groove (13), the inside of cultivateing between groove (13) is provided with between groove supporting seat (14), the inside of between groove supporting seat (14) is provided with cultivates jar and puts groove (18), the upper end that the groove (18) was put to the cultivateing jar is provided with transparent cultivation jar (15), the inside of transparent cultivation jar (15) is provided with charged disc (21), the top of charged disc (21) is provided with virus species culture solution (22), virus species culture solution (22) are pure water, The culture box comprises a mixed aqueous solution of amino acid, monosaccharide, vitamins, inorganic ions and trace elements, wherein a rear connecting plate (17) is arranged on the rear end face of a culture intermediate tank (13), a wireless network camera (19) is arranged on the front end face of the rear connecting plate (17), and a movable door sealing plate (7) is arranged on the front end face of the culture box (5).
2. The virus seed suspension culture box of claim 1, wherein: be provided with on the position that dodge gate shrouding (7) and incubator (5) are connected sealed groove (8), sealed groove (8) and incubator (5) integrated into one piece set up, all be provided with the direction on the both sides inner wall in sealed groove (8) and lead to groove (9), the direction leads to groove (9) and sealed groove (8) integrated into one piece setting, the inside that the groove (9) was led to in the direction is provided with transmission armed lever (10), the both ends of transmission armed lever (10) respectively with dodge gate shrouding (7) and electric putter's (4) output welded connection.
3. A seed suspension incubator as recited in claim 2, wherein: all be provided with logical groove inner wall groove (28) on the both sides inner wall of direction logical groove (9), logical groove inner wall groove (28) and direction logical groove (9) integrated into one piece set up, all be provided with armed lever inside groove (29) on the both sides outer wall of transmission armed lever (10), the inside of armed lever inside groove (29) is provided with supplementary gyro wheel (30), and transmission armed lever (10) pass through supplementary gyro wheel (30) and logical groove inner wall groove (28) roll connection.
4. A seed suspension incubator as claimed in claim 3, wherein: the inner walls of the two sides of the arm lever inner groove (29) are respectively provided with a bearing (31), the bearings (31) are fixedly connected with the arm lever inner groove (29) through screws, the outer walls of the two sides of the auxiliary roller (30) are respectively provided with a connecting shaft (32), the connecting shafts (32) and the auxiliary roller (30) are integrally formed, and the auxiliary roller (30) is movably connected with the bearings (31) through the connecting shafts (32) on the outer walls of the two sides.
5. The virus seed suspension culture box of claim 1, wherein: be provided with inner frame back plate (23) on the rear end position of cultivateing inner frame (6), be provided with four on the rear end inner wall of incubator (5) and correspond connecting rod (24), the both ends that correspond connecting rod (24) respectively with incubator (5) and inner frame back plate (23) welded connection, the top of incubator (5) is provided with power supply cabinet (16), the inside of inner frame back plate (23) is provided with twelve winding displacement and perforates (25), and winding displacement perforation (25) set up with inner frame back plate (23) integrated into one piece, and twelve winding displacement perforation (25) respectively with twelve cultivateing between groove (13) one-to-one, be provided with on the electricity connection end of wireless network camera (19) and connect electric wire (20).
6. A seed suspension incubator according to claim 5, wherein: four correspond to be provided with integrated row (26) of inserting on the position between connecting rod (24), integrated row (26) of inserting passes through screw and incubator (5) fixed connection, twelve connect electric wire (20) run through the winding displacement and perforate (25) and with integrated row (26) electric connection of inserting, integrated row (26) of inserting connect to be provided with power supply wiring (27) on the electric end, integrated row (26) of inserting is through power supply wiring (27) and power supply cabinet (16) electric connection.
7. The virus seed suspension culture box of claim 1, wherein: the incubator (5) is made of stainless steel, the thickness of the stainless steel is twenty centimeters, and the inner wall of the stainless steel is lined with ten centimeters of polyurethane foam plastic.
8. The virus seed suspension culture box of claim 1, wherein: the hand-operated device (3) is arranged on the front end face of the push rod control cabinet (1), the output end of the hand-operated device (3) is electrically connected with the input end of the push rod control cabinet (1), and the output end of the push rod control cabinet (1) is electrically connected with the input ends of the two electric push rods (4).
9. The virus seed suspension culture box of claim 1, wherein: be provided with wireless AP (33), router (34) and network video recorder (35) on the rear end outer wall of incubator (5), be provided with support platform (11) on one side outer wall of incubator (5), support platform (11) and incubator (5) integrated into one piece setting, the upper end of support platform (11) is provided with display (12), the output of wireless network camera (19) and the input transmission connection of wireless AP (33).
10. A seed suspension incubator as recited in claim 9, wherein: the output end of the wireless AP (33) is electrically connected with the input end of the router (34), the output end of the router (34) is electrically connected with the input end of the network video recorder (35), and the output end of the network video recorder (35) is electrically connected with the input end of the display (12).
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| CN202210138604.7A CN114540190A (en) | 2022-02-15 | 2022-02-15 | Poison seed suspension incubator |
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| CN202210138604.7A CN114540190A (en) | 2022-02-15 | 2022-02-15 | Poison seed suspension incubator |
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Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070231217A1 (en) * | 2005-12-21 | 2007-10-04 | Clinton Charles M | Assay apparatuses, methods and reagents |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| CN103458229A (en) * | 2013-09-04 | 2013-12-18 | 福建北讯智能科技有限公司 | Wireless video monitoring practical training device |
| US20150360226A1 (en) * | 2014-06-12 | 2015-12-17 | Wafergen, Inc. | Single cell capture with polymer capture films |
| CN105274001A (en) * | 2014-05-30 | 2016-01-27 | 奥林巴斯株式会社 | Medium exchange system |
| CN205719980U (en) * | 2016-04-26 | 2016-11-23 | 东北大学 | A kind of electrochemical in-situ raman spectroscopy measurement microscopic heating stand and sample cell system |
| CN107482519A (en) * | 2017-08-11 | 2017-12-15 | 王洁琳 | A kind of electric drawer formula Electromagnetic Control electric power cabinet |
| CN208127716U (en) * | 2018-04-27 | 2018-11-20 | 国网山西省电力公司太原供电公司 | A kind of Multifunctional assembled low-tension distribution box |
| CN208346189U (en) * | 2017-11-15 | 2019-01-08 | 中国航天员科研训练中心 | The image capturing system of automated cell culture device |
| CN109618524A (en) * | 2019-01-21 | 2019-04-12 | 天津市璐祥自动化设备股份有限公司 | A kind of PLC control cabinet convenient for wiring |
| CN110551632A (en) * | 2019-07-25 | 2019-12-10 | 南方医科大学珠江医院 | Real-time monitoring cell culture system and culture method thereof |
| CN210181104U (en) * | 2019-06-12 | 2020-03-24 | 江阴众和电力仪表有限公司 | Extensible single-phase electric energy meter case |
| KR102130680B1 (en) * | 2019-07-22 | 2020-07-06 | 김재민 | Magnetic a culture medium |
| CN210945559U (en) * | 2019-10-21 | 2020-07-07 | 上海鸣大生物科技有限公司 | Multifunctional biochemical incubator |
| CN211199187U (en) * | 2019-08-20 | 2020-08-07 | 天津普瑞思生物科技有限公司 | DNA purification is with crimping formula electrophoresis apparatus |
| CN112608826A (en) * | 2020-11-24 | 2021-04-06 | 浙江省农业科学院 | Incubator capable of observing growth condition of microorganisms |
| CN212925043U (en) * | 2020-06-14 | 2021-04-09 | 徐帅 | Food microorganism detects incubator |
| CN212934364U (en) * | 2020-10-11 | 2021-04-09 | 杨勇 | Antifouling formula transformer |
| CN214991655U (en) * | 2021-05-19 | 2021-12-03 | 天信和(苏州)生物科技有限公司 | Carrier and bioreactor |
-
2022
- 2022-02-15 CN CN202210138604.7A patent/CN114540190A/en active Pending
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070231217A1 (en) * | 2005-12-21 | 2007-10-04 | Clinton Charles M | Assay apparatuses, methods and reagents |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| CN103458229A (en) * | 2013-09-04 | 2013-12-18 | 福建北讯智能科技有限公司 | Wireless video monitoring practical training device |
| CN105274001A (en) * | 2014-05-30 | 2016-01-27 | 奥林巴斯株式会社 | Medium exchange system |
| US20150360226A1 (en) * | 2014-06-12 | 2015-12-17 | Wafergen, Inc. | Single cell capture with polymer capture films |
| CN205719980U (en) * | 2016-04-26 | 2016-11-23 | 东北大学 | A kind of electrochemical in-situ raman spectroscopy measurement microscopic heating stand and sample cell system |
| CN107482519A (en) * | 2017-08-11 | 2017-12-15 | 王洁琳 | A kind of electric drawer formula Electromagnetic Control electric power cabinet |
| CN208346189U (en) * | 2017-11-15 | 2019-01-08 | 中国航天员科研训练中心 | The image capturing system of automated cell culture device |
| CN208127716U (en) * | 2018-04-27 | 2018-11-20 | 国网山西省电力公司太原供电公司 | A kind of Multifunctional assembled low-tension distribution box |
| CN109618524A (en) * | 2019-01-21 | 2019-04-12 | 天津市璐祥自动化设备股份有限公司 | A kind of PLC control cabinet convenient for wiring |
| CN210181104U (en) * | 2019-06-12 | 2020-03-24 | 江阴众和电力仪表有限公司 | Extensible single-phase electric energy meter case |
| KR102130680B1 (en) * | 2019-07-22 | 2020-07-06 | 김재민 | Magnetic a culture medium |
| CN110551632A (en) * | 2019-07-25 | 2019-12-10 | 南方医科大学珠江医院 | Real-time monitoring cell culture system and culture method thereof |
| CN211199187U (en) * | 2019-08-20 | 2020-08-07 | 天津普瑞思生物科技有限公司 | DNA purification is with crimping formula electrophoresis apparatus |
| CN210945559U (en) * | 2019-10-21 | 2020-07-07 | 上海鸣大生物科技有限公司 | Multifunctional biochemical incubator |
| CN212925043U (en) * | 2020-06-14 | 2021-04-09 | 徐帅 | Food microorganism detects incubator |
| CN212934364U (en) * | 2020-10-11 | 2021-04-09 | 杨勇 | Antifouling formula transformer |
| CN112608826A (en) * | 2020-11-24 | 2021-04-06 | 浙江省农业科学院 | Incubator capable of observing growth condition of microorganisms |
| CN214991655U (en) * | 2021-05-19 | 2021-12-03 | 天信和(苏州)生物科技有限公司 | Carrier and bioreactor |
Non-Patent Citations (1)
| Title |
|---|
| 董润安: "《现代生物学》", 31 July 2016, 北京理工大学出版社 * |
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Application publication date: 20220527 |