KR100883401B1 - Composition formed by using Gyoungokgo and method for preparing the same - Google Patents

Abstract

Provided are compositions and methods for preparing the same, which are made using jadeite with improved antioxidant, cellular physiological activity, and / or anti-aging effects. The composition according to one embodiment of the present invention comprises a jadeite fermentation product. The method for preparing a composition according to an embodiment of the present invention includes providing a jadeite, and preparing the jadeite fermentation product by fermenting the jadeite.

Jadeite, fermentation product, antioxidant

Description

Composition formed by using Gyoungokgo and method for preparing the same}

1 is a flow chart illustrating a method of making a composition in accordance with some embodiments of the present invention.

Figure 2 is a flow chart of a method for manufacturing jadeite in accordance with an embodiment of the present invention.

Figure 3 is a flow chart of a method for producing a jadeite fermentation product according to an embodiment of the present invention.

4A is a graph showing HPLC analysis for jadeite.

4B is a graph showing HPLC analysis of jadeite fermentation products.

The present invention relates to a composition made using jadeite, and more particularly, to a cosmetic composition, a pharmaceutical composition and a composition for soap made using jadeite, and a method for producing the same.

The jadeite is a kind of drowning medication that helps life and improve blood, and according to consent, it is said to have a great effect on maintaining and activating the functions of all organs in the human body. In addition, the jadeite is not only a medicinal agent and a medicinal agent, but specifically, a patient with a weak stomach and fatigue, a stomach, a weak stomach, a severe indigestion, a long stomach pain and a severe stomachache, and a slight cold weather. It is said to be effective for patients who catch cold, breathe with bronchial asthma and have severe coughing of seawater. Especially, when the weak and weak are worn by the elderly, the gray hair is black, the teeth are newly sprouted and the life is extended. .

On the other hand, according to the results of modern research on jadeite, jadeite has an antioxidant effect, thereby contributing to cell physiological activity. In addition, jadeite is known to be effective in alleviating the skin aging phenomenon, improve skin wrinkles and have a skin whitening effect.

However, despite the above advantages, the jadeite has a long time in its manufacture, and the manufacturing method is very complicated, and thus there is a limit to the application to the modern industry. Although methods for simplifying the manufacturing method of jadeite are provided incessantly, they do not guarantee the efficacy as that of the traditional jadeite. Furthermore, even in the case of jadeite by the traditional manufacturing method is not enough antioxidant effect, skin wrinkle improvement effect, skin whitening effect, etc., there is a point that it is still insufficient to be used as a material of modern medicine, cosmetics, soap or the like.

The technical problem to be achieved by the present invention is to provide a composition made using jadeite improved in antioxidant, cell physiological activity, and / or skin aging efficacy.

Another technical problem to be achieved by the present invention is to provide a method for producing the composition as described above.

Technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description.

The composition according to an embodiment of the present invention for achieving the above technical problem includes a jadeite fermentation product.

Method of producing a composition according to an embodiment of the present invention for achieving the above another technical problem includes providing a jadeite, and fermenting the jadeite comprises preparing a jadeite fermentation product.

Specific details of other embodiments are included in the detailed description and the drawings.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various forms, and only the embodiments make the disclosure of the present invention complete, and the scope of the invention to those skilled in the art. It is provided for the purpose of full disclosure, and the invention is only defined by the scope of the claims. Thus, in some embodiments, well known process steps, well known device structures and well known techniques are not described in detail in order to avoid obscuring the present invention. Like reference numerals refer to like elements throughout.

Compositions according to embodiments of the present invention include jadeite fermentation products. The jadeite fermentation product is a product obtained by fermenting the jadeite. The jadeite is derived from a combination of ginseng, yohwang, bokyeong, and buckwheat.

Ginseng includes ginseng saponins (ginsenoside Ra, Rb1, Rb2, Rb3, Rc, Rd, Re, Ro) having various physiological activities. It also contains many types of amino acids, sugars, vatamin, flavonoids, volatile essential oils and minerals. Ginseng has weakness due to lack of energy, immunity enhancement, memory loss, boredom, fatigue, sweaty symptoms and comfort of mind, and excellent effects on mental strength, thinking power, and erectile dysfunction. It is used as an anti-diabetic and analgesic medicine. The pharmacological action of ginseng includes hypoglycemic action, anticancer effect, serum protein synthesis promotion, antibody production promotion action, central excitement action, anti-fatigue action. In addition, it is effective for psychogenic shock, and has a significant anti-cancer effect in patients with gastric cancer surgery in the middle and end stages, and is effective for erectile dysfunction, premature ejaculation, shortage of ejaculation, and decreased sexual function. It works. Ginseng referred to herein includes red ginseng.

Bokryeong may be Poria cocos (Fr) Wolf, and may mean that the bacterium of Bokryeong is dried. Rehabilitation includes beta-pachyman. In addition, Bokryeong contains a number of proteins, glucose, and the like. Fukryeong is not good for urine and swelling of the stomach and the body is effective for the treatment of sea water, vomiting, diarrhea, forgetfulness, oil well. In addition, Bokryeong has a remarkable response to renal edema. In addition, it has an effect of preventing the formation of ulcers, and has a potent sugar strengthening and contact dermatitis suppressing action.

The rehmannia may be composed of, for example, the roots of perennial perennial Rehmannia glutinosa Liboschitz and Rehmannia glutinosa Libosch.for.hueichingensis Hshlao. For example, it may be classified as raw and live sulfur. Exemplary embodiments of the present invention use raw sulfur as said sulfur, but do not exclude the application of sulfur. The components of the turmeric include beta-sistosterol, campesterol, rehmanin, alkaloids, fatty acids and the like. Digestive pharmacologically lowers blood sugar, acts on the circulatory system, has a strong and diuretic effect, protects liver function, and has antibacterial effects, and can be applied to suppress diabetes, hemostasis, nosebleeds, uterine bleeding, menstrual irregularities, and constipation. have.

White wheat may refer to honey or starch syrup. White wheat prevents the oxidation of sulfur and wormling, and at the same time serves to soften the taste and help digestion. In addition, white wheat stops the pain and has the effect of treating sea water and constipation.

A jadeite is a concentrate or an extract thereof, which is concentrated by bathing a combination of ginseng, jihwang, bokyeong, and buckwheat as described above. With the combination of the ginseng, jihwang, Bokryeong, and Bokmil, the jadeite has various pharmacological activities. In particular, it has an effective effect on antioxidant, cell activity, skin wrinkle improvement, skin whitening and the like.

The jadeite fermentation product is a product obtained by fermenting the jadeite. Fermentation of the jadeite uses, for example, fermentation microorganisms. Fermented microorganisms include bacteria, fungi. Specific examples of jadeite fermentation include alcohol fermentation by yeast, lactic acid fermentation by lactic acid bacteria, formic acid fermentation by enteric bacteria, butyric acid-butanol-acetone fermentation by Clostridium bacteria, propionic acid fermentation by propionic acid bacteria, or methane bacteria Methane fermentation by etc. are mentioned. However, of course, it is not limited to the above example.

The jadeite fermentation product according to some embodiments of the present invention may not include the fermentation microorganisms used in the fermentation. Some other embodiments of the present invention may further comprise the fermentation microorganisms described above, such as in trace amounts.

Under the influence of the fermentation, the jadeite fermentation product according to the embodiments of the present invention has a different content of the active ingredient as compared to the unfermented jadeite. For example, some active ingredients increase in content. With such an increase in the amount of active ingredients, jadeite fermentation products are related to the effects of antioxidant, cell activity, skin wrinkle improvement, skin whitening and the like better than jadeite. That is, the jadeite fermentation product described above will be confirmed through the following experimental example, but has more excellent efficacy than the jadeite in antioxidant, cell activity, skin wrinkle improvement, skin whitening, etc., and is excellent in terms of safety. Furthermore, even if jadeite is produced by a simple method without using a conventional manufacturing method and its efficacy is weakened, by applying as a fermentation product as described above, the weakened efficacy can be compensated for. That is, since the efficacy of the jadeite can be improved or at least applied in a state in which the weakening of the efficacy is suppressed, it can be substantially possible to manufacture the jadeite in large quantities in a simpler manner.

In addition, the composition comprising a jadeite fermentation product according to embodiments of the present invention has a pH of less than 7.0 under the influence of the fermentation. The pH of the composition may vary depending on the degree of fermentation of the jadeite, the content of the jadeite fermentation product, and the like, for example, may have a range of 2.0 to 6.5. Compositions according to some embodiments of the present invention may have a pH of 4.0 to 6.0.

Such jadeite fermentation product may have a content of 0.0001 to 30% by weight relative to the total weight of the composition according to the embodiments of the present invention. Preferably the jadeite fermentation product may have a content of 0.001 to 20% by weight relative to the total weight of the composition.

If necessary, the composition according to some embodiments of the present invention may further include an additive. For example, medicinal products such as ginseng, succinate, wild ginseng and camphor ginseng may be further included in the jadeite fermentation product. Furthermore, the composition according to embodiments of the present invention may further comprise various other materials such as carriers and the like depending on the application applied.

More specifically, the composition according to some embodiments of the present invention may be applied to the cosmetic composition using the effects of excellent antioxidant, cell activity, skin wrinkle improvement, skin whitening, stability, etc. of the jadeite fermentation product. In this case, the composition may have any one formulation selected from the group consisting of lotion, essence, lotion, cream, pack, gel, ointment, patch, and spray.

When the composition is applied as a cosmetic composition, the composition may further comprise a carrier acceptable to the cosmetic, in addition to the jadeite fermentation products described above. The cosmetically acceptable carrier may be included in cosmetic preparations, and includes those having a degree of toxicity, stability, and irritation requirements to which the human body is adapted to contact with the skin. Carriers acceptable in cosmetics can be, for example, alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, perfumes, and the like, and one or more cosmetic agents selected from these. Carriers acceptable to may be included in the composition, but are not limited thereto.

The content of the carrier acceptable in the cosmetics relative to the total weight of the composition may be, for example, 1 to 99% by weight, preferably 90 to 99% by weight. However, the present invention is not limited thereto, and the content may vary depending on the formulation of the above-described composition or the site to which the composition is applied.

In addition, the composition according to another embodiment of the present invention can be applied as a pharmaceutical composition utilizing the effects of excellent antioxidant, cell activity, skin wrinkle improvement, skin whitening, safety, etc. of the jadeite fermentation product. The jadeite fermentation product included in the composition may further have a pharmacological effect such as acne improvement, atopic dermatitis improvement, skin trouble improvement, or moisturizing.

When the composition is applied as a pharmaceutical composition, it may have any one formulation selected from the group consisting of solutions, suspensions, emulsions, elixirs, extracts, powders, granules, tablets, warnings, ointments, lotions, and ointments. have. Pharmaceutical compositions comprising a jadeite fermentation product may be administered to the human body in an oral or parenteral manner. Preferably it may be administered directly to the skin in a topical manner. In this case, the dosage may be, for example, 0.001 to 150 mg / kg per day, and may be administered once or in several divided doses. However, the dosage is only one example and may be modified according to the route of administration, the age, sex, weight, severity, etc. of the patient.

The pharmaceutical composition as described above may further comprise a pharmaceutically acceptable carrier in addition to the jadeite fermentation product described above. The pharmaceutically acceptable carrier may be included in the medicament, and includes those having the toxicity, stability, and irritant requirements that the human body can adapt to when administered to the human body. Such pharmaceutically acceptable carriers include, for example, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinyl pyrrolidone, Cellulose, water, syrup, methyl cellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate, mineral oil, and the like, and one or two or more pharmaceutically acceptable carriers selected from these may be added to the composition. May be included. However, of course, it is not limited to the above example.

Furthermore, the composition according to the present embodiments may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like as a pharmaceutical additive.

The content of the above-mentioned pharmaceutically acceptable carrier relative to the total weight of the composition may be, for example, 1 to 99% by weight, preferably 90 to 99% by weight. In addition, the content of the aforementioned pharmaceutical additives relative to the total weight of the composition may be, for example, 0.1% to 20% by weight. However, the content of the pharmaceutically acceptable carrier and the pharmaceutical additives is not limited to the above content range, and the content may vary depending on the formulation of the composition or the manner in which the composition is administered. Of course.

In addition, the composition according to another embodiment of the present invention can be applied as a composition for soap using the effects of excellent antioxidant, cell activity, skin wrinkle improvement, skin whitening, safety and the like of jadeite fermentation products. In this case, the composition may further include a soap base and / or soap additives, in addition to the jadeite fermentation product described above. Additives for soaps may include, for example, at least one of skin moisturizers, emulsifiers, and water softeners.

As the soap base material, for example, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal fats and oils such as tallow, pork, sunny and fish oil may be applied.

The skin moisturizer may be, for example, glycerin, erythritol, polyethylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol, and the like.

As the emulsifier, for example, natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts and the like can be applied.

As the water softener, tetrasodium editieti and the like can be exemplified.

In addition to the above-described additives for soap, the composition for soap according to the present embodiment further includes an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like. can do.

The content of the soap base relative to the total weight of the composition may be, for example, 50 to 99.999% by weight, and the content of the soap additive may be 1 to 20% by weight, but is not limited thereto.

Hereinafter, a method for preparing a composition according to the embodiments of the present invention as described above will be described. 1 is a flowchart illustrating a method of preparing a composition according to embodiments of the present invention.

Referring to Figure 1, first to provide a jadeite (S1). The jadeite may be prepared and provided by a method described in a book such as Dongbogam or the like which is well known in the art. It may also be provided by obtaining a jadeite prepared in advance. Here, the jadeite provided above may be included as long as it can be referred to in the art as a jadeite despite the difference in the manufacturing method, content, and efficacy thereof.

Reference is now made to FIG. 2 to illustrate an exemplary method for providing jadeite. FIG. 2 is a view for explaining an exemplary method of directly preparing and providing jadeite, and is a flowchart of a method of manufacturing jadeite according to an embodiment of the present invention.

Referring to FIG. 2, first, ginseng, bokyeong, jihwang, and white wheat are blended (S11). The blending ratio thereof may be, for example, 7,000 g to 12,000 g of raw sake juice, 5,000 g to 7,000 g of white wheat flour, 800 g to 1,200 g of ginseng powder, and 1,500 g to 2,000 g of Bokryeong powder. For example, the compounding ratio may be 7.3 kg of saengjihwang juice (juice juice with 16 knots of saengjihwang), white wheat 6kg (10g), 900g (24g) of ginseng powder, and 1,800g (48g) of Bokyong powder. . However, the compounding ratio is not limited to the examples listed above, of course, can be added or subtracted as necessary.

Subsequently, the blend is primaryly bathed (S12). The primary bath is, for example, put the compound in a frying jar, as described in Dongbobogam, seal the jar and agar with four layers of fat and a thick cloth and hang it in water in a copper pot, but the agar of the jar comes out of the water. This can be done by boiling three days a night with a mulberry fire. However, the present invention is not limited to the above specific terms, and the primary bath may be proceeded by placing the blend in a sealed container and treating the bath at a temperature of 100 ° C. or less for about 3 days.

Next, the compound is cooled (S13). The cooling time may be for example about 1 day. In the synonym, it is described that the primary hot water pot is suspended in a well for one day, but the method is not limited thereto.

Subsequently, the cooled mixture is secondary bathed (S14). The secondary bath is proceeded substantially the same as the primary bath, but can only remove the remaining water by bathing for about 1 day. As a result, jadeite is produced.

Referring back to Figure 1, by fermenting the jadeite provided to prepare a fermentation product (S2). Reference is made to FIG. 3 to describe an exemplary method of performing the S2 step. Figure 3 is a flow chart of a method for producing a jadeite fermentation product according to an embodiment of the present invention.

Referring to Figure 3, first to provide a dilution of jadeite dilution jadeite (S21). The jadeite diluent can be provided, for example, by adding jadeite to the organic solvent or by adding jadeite to the liquid medium. The liquid medium may be one capable of culturing the fermentation microorganism described below. The amount of jadeite included in the diluent of jadeite may be, for example, 1 to 20%. One preferred embodiment may be about 5%.

Subsequently, the fermentation microorganism is supplied to the diluent of jadeite and cultured thereto (S22). As the fermentation microorganism, any microorganism capable of fermenting the light jade, for example, yeast, lactic acid bacteria, enteric bacteria, Clostridium bacteria, propionic acid bacteria, methane bacteria, fungi and the like is applicable. Moreover, two or more microorganisms can also be supplied as a fermentation microorganism. In this specification, Paecilomyces japonica is disclosed as a specific example of the fermentation microorganism not limiting the present invention.

The jadeite diluent supplied with the fermentation microorganism is cultured according to the culture conditions of the fermentation microorganism. For example, it may be incubated for 2 hours to 7 days at a temperature of 10 to 40 ℃. In the case of using the pacillomyces japonica as fermentation microorganisms, it can be incubated with stirring at a speed of 140 rpm for 96 hours at a temperature of 24 ℃. As a result of the culture of the fermentation microorganisms as described above, at least a part of the jadeite fermentation is fermented. The result is a jadeite fermentation product. The resulting jadeite fermentation product varies in content of active ingredient and may have a pH of 2 to 6.5.

Then, optionally, the following steps S23 and S24 may be further processed. That is, by distilling the dilution of the jadeite distillation cultured fermentation microorganisms to sink the fermentation microorganisms (S23).

Subsequently, the supernatant is filtered to remove fermentation microorganisms that may remain in the supernatant (S24). The filtration may, for example, be accomplished using a filter having a pore size of 0.1 to 1 μm.

Subsequently, by diluting the filtered jadeite fermentation product, a composition comprising the jadeite fermentation product can be prepared. Furthermore, if necessary, the method may further include mixing various carriers or additives with the prepared composition.

For example, when the prepared composition is applied as a cosmetic composition, a carrier acceptable to the cosmetic may be further mixed with the composition. In addition, when the prepared composition is applied as a pharmaceutical composition, a pharmaceutically acceptable carrier and / or pharmaceutical additives may be further mixed with the composition. In addition, when the prepared composition is applied as a composition for soap, it is possible to further mix the soap base and / or soap additives in the composition. The carrier, pharmaceutically acceptable carrier, pharmaceutical additive, soap base, soap additive, which are acceptable in the cosmetics to be mixed have already been mentioned, and thus redundant description is omitted. In addition, since these specific mixing methods are known in the art, description thereof will be omitted in order to avoid obscuring the present invention.

More detailed description of the present invention will be described through the following specific preparation examples and experimental examples, and details not described herein will be omitted because those skilled in the art can sufficiently infer technically.

< Production Example  1> Preparation of Liquid Medium

2% (w / v) of agar was added to the medium composition having the culture component of pacillomyces japonica, the strain to be inoculated, and autoclaved at 121 ° C. and 15 psi for about 20 minutes. Next, 20 ml of the autoclaved liquid medium was pre-sterilized in a flask to prepare a liquid medium. The prepared liquid medium showed an optimum culture state at a temperature of 24 ± 1 ° C. and a pH of 6.0 to 7.0.

< Production Example  2> Jadeite  Preparation of Fermentation Products

To the liquid medium prepared according to Preparation Example 1, jadeite was added to have a concentration of 5%. Subsequently, the liquid medium was inoculated with the previously cultured Pasylomyces japonica strain and incubated for 96 hours with stirring at a speed of 140 rpm at a temperature of 24 ° C. Next, the culture medium in which the pacillomyces japonica strain was cultured was centrifuged to subside the strain, and the supernatant was taken. Subsequently, in order to remove the microbe remaining in a supernatant liquid, it filtered using the filter paper which has a pore size of 0.2 micrometer. As a result, a jadeite fermentation product was produced.

< Experimental Example  1> Jadeite  Physical of ingredients in fermentation products Change of appearance

HPLC (High Performance Liquid Chromatography) test was performed to analyze the components of the jadeite fermentation product prepared in Preparation Example 2. First, 100 mg of jadeite fermentation product was dissolved in 10 ml of 80% methanol, which was centrifuged to obtain a supernatant. The supernatant was then filtered through a membrane filter with a pore size of 0.2 μm. The filtrate was visually inspected for its color and pH was examined.

In addition, as a comparative example, visual inspection and pH irradiation were performed on the jadeite under the same conditions as described above. The results are shown in Table 1 below.

Experimental group color pH Jadeite Brown 7.0 Jadeite fermentation products Light brown 4.0

Referring to Table 1, it can be seen that the jadeite fermentation product prepared in Preparation Example 2 has a color change compared to jadeite. In addition, it can be seen that the jadeite fermentation product has a lower pH than jadeite.

< Experimental Example  2> Jadeite  Of the components contained in the fermentation product HPLC  Test

HPLC (High Performance Liquid Chromatography) test was performed on the jadeite fermentation product and the filtrate of jadeite according to Experimental Example 1. HPLC tests were performed using HPLC systems including SCL-10A controller (Shimmadzu, Japan), LC-10AD pump (Shimmadzu, Japan) and SPD-10A UV detector (Shimmadzu, Japan). At this time, the column was RP-18 reverse phase (Mightysil GP 250-4.6, Kanto. Japan,), the column temperature was 40 ℃, 75% acetonitrile (acetonitril) was used as a solvent. In addition, flow rate conditions were carried out at 1 ml / min, detection conditions at 218nm. The results are shown in FIGS. 4A and 4B.

4A is a graph showing HPLC analysis for jadeite. 4B is a graph showing HPLC analysis of jadeite fermentation products. 4A and 4B, it can be seen that the graph of FIG. 4B shows higher peaks in some corresponding sections than the graph of FIG. 4A. Accordingly, it can be seen that the jadeite fermentation product has an increased content of some active ingredients compared to jadeite.

< Experimental Example  3> Jadeite  Determination of Antioxidant Effects of Fermentation Products

In order to measure the antioxidant effect of the jadeite fermentation product prepared in Preparation Example 2, a free radical scavenging activity test was conducted. Specifically, three tubes containing 0.2 mM DPPH (1,1-diphenyl-2-picryhydrazyl, Sigma) solution 1 ml each were prepared. One tube was added purified water as a control, and the other two tubes were respectively added jadeite and jadeite fermentation products prepared according to Preparation Example 2, respectively, as experimental groups. Then, after leaving each of the three tubes at room temperature for 10 minutes, the absorbance was measured at 525 nm for the three tubes, and then the free radical scavenging ratio of the experimental group was calculated using Equation 1 below. The results are shown in Table 2 below.

% Free radical scavenging ratio = [100-{(absorbance of experimental group-absorbance of control group) / absorbance of control group} × 100]

Experimental group Free radical scavenging rate (%) Experimental group added with jadeite 16.6 Experimental group added with jadeite fermentation product 50.4

As can be seen from Table 2, the jadeite fermentation product prepared in Preparation Example 2 exhibited about 3 times more free radical scavenging rate than jadeite. From this, it can be seen that jadeite fermentation products have a much better antioxidant effect than jadeite.

< Experimental Example  4> Jadeite  Inhibitory Effect of Fermentation Products on Melanin Production

First, 24-well plate medium to which 10% fetal bovine serum was added to Dulbecco's modified Eagle's media (DMEM) was prepared. Rat cells were inoculated with B16 melanoma cell line at a concentration of 10 ⁵ cells / well and cultured under 37 ° C. and 5% CO 2 conditions. Subsequently, after confirming the adhesion of the pigment cells, the jadeite and jadeite fermentation products prepared in Preparation Example 2 were added to the medium as an experimental group, respectively, and cultured for 3 days, respectively. In addition, as a control, the medium without addition of the jadeite and jadeite fermentation products was incubated together for 3 days.

Subsequently, the culture solution was removed from the experimental group and the control group, respectively, and washed with PBS. Subsequently, the pigment cells were dissolved with 1N sodium hydroxide, and the absorbance was measured at 400 nm, and then the melanin production inhibition rate was calculated according to Equation 2 below. The results are shown in Table 3 below.

Melanin production inhibition rate (%) = [100- (absorbance of experimental group / absorbance of control group) × 100]

Experimental group Melanin production inhibition rate (%) Experimental group added with jadeite 20.6 Experimental group added with jadeite fermentation product 51.3

It can be seen from Table 3 that the jadeite fermentation product prepared in Preparation Example 2 has a superior effect of inhibiting melanin production of pigment cells than jadeite.

< Experimental Example  5> Jadeite  Effect of Fermentation Products on Human Fibroblast Collagen Biosynthesis

First, 96-well plates in which 10% fetal bovine serum was added to Dulbecco's modified Eagle's media (DMEM) were prepared. Human fibroblasts were inoculated on the plate at a concentration of 5,000 cells / well and incubated at 37 ° C. and 5% CO 2 conditions until 70% to 80% growth. Subsequently, the jadeite and the jadeite fermentation product prepared in Preparation Example 2 were respectively added to the plate as an experimental group, and each was incubated for 1 day. Each of the cultured cell cultures was collected and the degree of collagen production was measured using a commercially available collagen biosynthesis measuring instrument (Japan, Takara Shuzo Co., Ltd. Catalog #: MK101).

Specifically, the collected cell culture solution was placed in a 96-well plate uniformly coated with primary collagen antibodies, and then subjected to an antigen-antibody reaction in a thermostat for 3 hours. Thereafter, the secondary collagen antibody conjugated with the chromophore was put into a 96-well plate and reacted again for 15 minutes. Then, the color causing substance was added to cause color development at room temperature for 15 minutes. Subsequently, 1M sulfuric acid was added to stop the color development. The absorbance was measured at 450 nm using a light absorber with a 96-well plate which stopped color development. Subsequently, the degree of collagen production was calculated by substituting the same into Equation 3 below. The results are shown in Table 4 below. In the following Equation 3, the absorbance of the control group was measured using a plate without jadeite and jadeite fermentation products.

Collagen production rate (%) = (Reaction absorbance of the experimental group / reaction absorbance of the control group) × 100

Experimental group Collagen production rate (%) Experimental group added with jadeite 18.9 Experimental group added with jadeite fermentation product 52.4

From Table 4, the jadeite fermentation product prepared by Preparation Example 2 has much better collagen production rate than jadeite, and it can be seen that collagen biosynthesis effect is much better.

< Experimental Example  6> Jadeite  Human Fibroblast Proliferation Effect of Fermentation Products

As an experimental group, a human fibroblast culture medium to which jadeite was added and a human fibroblast culture medium to which the jadeite fermentation product prepared in Preparation Example 2 were added were prepared. MTT reduction method [3 (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide reduction method] was performed on the prepared experimental group, and formazan product was extracted with isopropyl alcohol at 570 nm. Absorbance was measured. The cell proliferation was calculated by substituting this in Equation 4 below, and the results are shown in Table 5 below. In Equation 4 below, the absorbance of the control group was measured for human fibroblast culture medium to which no jadeite or jadeite fermentation product was added.

Cell proliferation (%) = (absorbance of experimental group / absorbance of control group) × 100

Experimental group Cell proliferation (%) Experimental group added with jadeite 13.2 Experimental group added with jadeite fermentation product 45.1

From Table 5, the jadeite fermentation product prepared according to Preparation Example 2 can be seen that the human fibroblast proliferation ability is much better than jadeite.

< Experimental Example  7> Jadeite  Cytotoxicity Test of Fermentation Products

In order to verify the cytotoxicity of the jadeite fermentation product prepared by Preparation Example 2, V79-4 cells (Chinese hamster, continuous cell line of lung tissue fibroblasts) were cultured with respect to the jadeite fermentation product, and references ( The MTT test was performed by the method described in Mossman T. Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays.Journal of Immunological Methods, 1983, 65, 55-63). The results are shown in Table 6 below.

sample Concentration killing 50% of cells [IC ₅₀ (%, w / v)] SLS 0.005 Jadeite fermentation products 10

As can be seen from Table 6, the IC ₅₀ value of the jadeite fermentation product prepared in Preparation Example 2 was about 2000 times or more than that of SLS. Thus, it can be seen that the cytotoxicity of the jadeite fermentation product is very low.

< Experimental Example  8> Jadeite  Of fermentation products allergy  evaluation

In order to confirm allergy-induced jadeite fermentation product prepared by Preparation Example 2, LLNA (Local Lymph Node Assay) method was used [Reference: OECD GUIDELINE FOR THE TESTING OF CHEMICALS; DRAFT NEW GUIDELINE 429: Skin Sensitization; Local Lymph Node Assay.

Specifically, the jadeite fermentation product is prepared in a solution of 20%, 40%, 60%, and prepared a solution of 60% ethanol as a control, and then prepared by the concentration of jadeite fermentation product and ethanol solution mouse (Balb / c) 25 μl was applied to both ears of 3 days. Thereafter, an isolated lymph node was isolated from the mouse, and then the isolated lymph node was pulverized into a single cell state. Subsequently, radioisotope [ ³ H] -methylthymidine was added to incubate for 24 hours, and then amplification degree (dpm) of cell proliferation was measured. The results are shown in Table 7 below.

Sample group Lymph node count (cells / mL × 10 ⁷ ) dpm (average) S.I. (amplitude) Ethanol (60%) 2.27 1866 Transportation Jadeite fermentation product (20%) 1.22 1011 0.54 Jadeite fermentation product (40%) 1.32 1254 0.67 Jadeite fermentation product (60%) 1.57 1876 1.01

In Table 7, the disintegration per minute (dpm) represents the amount of [ ³ H] -methylthymidine is inserted into the cells, and the SI (Stimulation Index) represents the average dpm of the sample divided by the average dpm of the control group.

The allergy evaluation result in Table 7 is shown to be lymphocyte amplification of the sample group compared to the control group, and even at one concentration, if the amplification degree (SI) of the sample is 3 or more and shows a tendency to increase in a concentration-dependent manner, it is regarded as an allergen. As described in 6, the jadeite fermentation product was found to have an amplification degree (SI) of 3 or less regardless of the concentration, indicating that there is no possibility of allergy.

< Production Example  3> Jadeite  Containing fermentation products Cosmetic  Preparation of the composition

To prepare a cosmetic composition as a composition comprising the jadeite fermentation product prepared in Preparation Example 2, a raw material as described in Table 8 below was prepared. First, raw materials No. 1 to 7 of Table 8 were mixed and dissolved at 70 ° C. to prepare a first part. In addition, raw materials No. 8 to 14 of Table 8 were mixed and dissolved at 70 ° C. to prepare a second part. The prepared second part was added to the first part, and the mixture was first emulsified by using a homomixer (Tokushu Kika, Japan), and then increased by adding raw material No. 15 of Table 8 below. Subsequently, bubbles were removed from the increased composition, and then cooled to room temperature to prepare a cosmetic composition of the cosmetic preparation.

Raw material number ingredient Input amount (g) One Purified water 100 2 glycerin 8.0 3 Butylene glycol 4.0 4 Hyaluronic acid extract 5.0 5 Beta Glucan 7.0 6 Carbomer 0.1 7 Jadeite fermentation product prepared by Preparation Example 2 3.0 8 Caprylic Capric Triglycerides 8.0 9 Squalane 5.0 10 Cetearyl Glucoside 1.5 11 Sorbitan stearate 0.4 12 Cetearyl Alcohol 1.0 13 antiseptic Quantity 14 incense Quantity 15 Triethanol amine 0.1

< Production Example  4> Jadeite  Containing fermentation products Cosmetic  Preparation of the composition

A cosmetic composition was prepared in the same manner as in Preparation Example 3, except that 1.5 g of the seventh raw material of Table 8 was not 3 g.

< Comparative Production Example  1 and 2> Jadeite  Does not contain fermentation products Cosmetic  Preparation of the composition

Except not mixing the raw material No. 7 of Table 8 to prepare a cosmetic composition according to Comparative Preparation Examples 1 and 2 in the same manner as in Preparation Example 3.

< Experimental Example  9> Cosmetic  Whitening effect of the composition on human skin

Twelve healthy men were placed on an opaque tape with four 1.5 cm diameter holes in the upper forearm of the subject, and then irradiated with 1.5 to 2 times the UV rays of each subject's minimum erythema (Minimal Erythema Dose). To induce blackening of the skin. Subsequently, the contrast of the blackened skin (L1: the darker the skin, the smaller the value) was measured using the color difference meter.

Subsequently, the cosmetic compositions according to Preparation Example 3, Preparation Example 4, and Comparative Preparation Examples 1 and 2 were applied to the blackened skin every day for two months, two mornings and two evenings. Then, the light and dark (L2) of the blackened skin was measured using a color difference meter. After calculating the difference between the contrast using the following Equation 5 based on the measurement results, the average of the difference between the contrast for each subject is shown in Table 9 below.

Difference in contrast (ΔL) = L1 (contrast before application of cosmetic composition) -L2 (contrast after application of last cosmetic composition)

Average value of ΔL for each subject Preparation Example 3 4.23 Preparation Example 4 2.52 Comparative Production Example 1 1.43 Comparative Production Example 2 1.21

As can be seen from Table 9, the contrast difference according to Preparation Example 3 and Preparation Example 4 was much greater than about two times than Comparative Preparation Example 1 and Comparative Preparation Example 2. Therefore, it can be seen that the whitening effect of the cosmetic composition containing the jadeite fermentation product is much greater. Furthermore, the cosmetic composition according to Preparation Example 3, in which 3 g of jadeite fermentation product was added, was found to have a greater whitening effect than the cosmetic composition according to Preparation Example 4, in which 1.5 g of jadeite fermentation product was added. It can be estimated that it is closely related to the content of.

< Experimental Example  10> Cosmetic  Wrinkle improvement effect on human skin of the composition

Eighty women in their 30s and 40s were classified into four groups, followed by replicating using silicone and skin wrinkles of each group of subjects through image analysis using a visiometer (SV500, Courage + Khazaka electronic GmbH, Germany). The depth was measured. In addition, by using a cutometer (SEM474, Courage + Khazaka electronic GmbH, Germany) capable of measuring skin elasticity, the elasticity of the facial region of each group of subjects was measured. In addition, the moisturizing power of each group of subjects was measured using a coneometer (CM820, Courage + Khazaka electronic GmbH, Germany).

Subsequently, the composition for the cosmetics according to Preparation Example 3, Preparation Example 4, and Comparative Preparation Examples 1 and 2 was provided to the examinee for each group. Then, the cosmetic composition provided to each subject was applied to the skin once a day for one month. After one month, the skin wrinkle depth, facial area elasticity, and moisturizing power of each group of subjects were measured. Using the measurement results, the average wrinkle reduction rate, average skin elasticity improvement rate, and average moisturizing power increase rate before and after application were calculated for each cosmetic composition, and the results are shown in Table 10 below.

Average Wrinkle Reduction (%) Average skin elasticity improvement (%) Average Moisturizing Growth Rate (%) Preparation Example 3 77 82 81 Preparation Example 4 69 64 82 Comparative Production Example 1 40 45 51 Comparative Production Example 2 9 10 11

As can be seen from Table 10, the cosmetic composition prepared by the method according to Preparation Example 3 and Preparation Example 4 wrinkle reduction rate, skin elasticity improvement, and moisturizing power than the case according to Comparative Preparation Example 1 and Comparative Preparation Example 2 All were found to be excellent in terms of increase. Therefore, it can be seen that the cosmetic composition containing the jadeite fermentation product is more excellent in improving wrinkles on human skin.

< Experimental Example  11> Cosmetic  Safety check of the composition

In order to confirm the safety of the cosmetic composition according to Preparation Example 3, Preparation Example 4, and Comparative Preparation Examples 1 and 2, the human skin cumulative stimulation test was performed.

Specifically, for each of the cosmetic composition according to Preparation Example 3, Preparation Example 4, and Comparative Preparation Examples 1 and 2 to 20 healthy adults, a total of nine 24-hour cumulative patch on the upper forearm every other day It was. As the patch method, a Fin chamber method was used, and 15 µl of the cosmetic composition was dropped per chamber, and the patch was performed. The reactivity of the skin was divided into ±, +, and ++ according to the degree, and 1, 2, and 4 (highest scores) were given to each. In addition, the average reactivity was calculated according to Equation 6 below, and is shown in Table 11 below with the results of the reactivity. In Table 11 below it is determined to be a safe composition when the average reactivity is less than 3.

Average responsiveness = (number of respondents × responsiveness / total number of subjects × highest score × 100) / total number of tests

"-" In Table 11 indicates that there is no corresponding person. As can be seen from Table 11, the cosmetic composition prepared by the method according to Preparation Example 3 and Preparation Example 4 shows no cumulative stimulation pattern at all like the cosmetic composition according to Comparative Preparation Example 1 and Comparative Preparation Example 2 Did. Therefore, it can be seen that the cosmetic composition according to Preparation Example 3 and Preparation Example 4 is excellent in safety.

< Production Example  5 to 8 Jadeite  Preparation of composition for soap containing fermentation product

To prepare a composition for soap as a composition comprising a jadeite fermentation product prepared by Preparation Example 2, a raw material as described in Table 12 below was prepared. Units of content in the following table are parts by weight.

ingredient Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Sodium soap 35 35 35 35 Propylene glycol 12 12 12 12 glycerin 8 8 8 8 Sugar 10 10 10 10 Polyoxyethylene (3) Lauryl Sulfate 10 10 10 10 Polyoxyethylene (5) Lauryl Ether One 5 10 - Polyoxyethylene (12) Lauryl Ether _ - - 5 Polyoxyethylene (20) Lauryl Ether _ One - - Tetrasodium ID 0.1 0.1 0.1 0.1 Jadeite fermentation product prepared by Preparation Example 2 One 3 5 10 incense One One One One Purified water 100 100 100 100

First, each component of Table 12 was mixed and heated and stirred until it became a transparent liquid at a temperature of 75 to 80 ℃. Subsequently, the transparent liquid composition was cooled to a temperature of 60 to 65 ° C, 1.0 parts by weight of fragrance was added and mixed and stirred uniformly. Then, it was poured into a mold of a certain shape to cool and solidify. As a result, a transparent soap containing jadeite fermentation product was produced.

Although the embodiments of the present invention have been described above with reference to the accompanying drawings, those skilled in the art to which the present invention pertains may be embodied in other specific forms without changing the technical spirit or essential features of the present invention. I can understand that. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive.

As described above, the composition according to the embodiments of the present invention includes a jadeite fermentation product, which not only has improved antioxidant, cellular physiological activity, and / or anti-aging effect of skin, but also has a very good effect in terms of safety. . Furthermore, even if the jadeite is manufactured by a simple method without using a conventional manufacturing method and its efficacy is weakened, the weakened efficacy can be compensated for, so that it is possible to manufacture a large amount of jadeite in a simpler manner substantially. have.

Claims (23)

delete delete delete delete A cosmetic composition comprising a jadeite fermentation product, The jadeite fermentation product is a cosmetic composition having a content of 0.0001 to 30% by weight relative to the total weight of the composition. The method of claim 5, A composition for cosmetics further comprising at least one selected from the group consisting of alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants and perfumes. The method of claim 5, The composition is a cosmetic composition comprising one formulation selected from the group consisting of lotion, essence, lotion, cream, pack, gel, ointment, patch, and spray. delete delete A soap composition comprising a jadeite fermentation product, The jadeite fermentation product soap composition having a content of 0.0001 to 30% by weight relative to the total weight of the composition. The method of claim 10, A soap composition further comprising at least one of a soap base, a skin moisturizer, an emulsifier, and a water softener. delete delete delete delete delete delete delete Provide jadeite, Fermenting the jadeite, to prepare a jadeite fermentation product, Of the composition comprising mixing at least one selected from the group consisting of alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants and flavorings. As a manufacturing method, The jadeite fermentation product is prepared to have a content of 0.0001 to 30% by weight relative to the total weight of the composition. delete The method of claim 19, And mixing at least one of a soap base, a skin moisturizer, an emulsifier, and a water softener to the jadeite fermentation product. delete delete

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