KR100589672B1 - Composition for inhibiting obesity and increasing stamina comprising mountain todok extract - Google Patents

Abstract

The present invention relates to a composition for inhibiting obesity and stamina enhancement, including the extract of Sandodeok, in particular, derived from natural plants, as well as safe for the human body, pharmaceutical products including the extract of Sandodeok excellent in the effect of inhibiting obesity and stamina enhancement And health functional foods, beverages, and foods.

Sanda Extract, Anti-Obesity, Stamina, Medicine, Health Food, Beverage, Food

Description

COMPOSITION FOR INHIBITING OBESITY AND INCREASING STAMINA COMPRISING MOUNTAIN TODOK EXTRACT}

1 is a schematic diagram of a process apparatus using ultrasonic vibrations used to prepare the extract of the present invention,

Figure 2 is a schematic diagram showing the overall process in the case of repeating the vacuum shock and ultrasonic vibrations used to prepare the sandalwood extract of the present invention.

The present invention relates to an anti-obesity and stamina enhancer composition comprising an extract, more particularly to an anti-obesity and stamina enhancer composition comprising an extract of the anti-obesity and a stamina enhancer activity.

As the economy develops and the diet becomes more foody, obesity is a threat to national health as people consume more processed foods with higher energy content and less exercise. Obesity is reported to be generally short-lived due to the high incidence and risk of surgery such as diabetes, arteriosclerosis, hypertension, heart disease, gallstones, gout and kidney disease.

Obesity affects all age groups, especially the elderly. It is known that obesity was not so much in the East due to the vegetarian-oriented lifestyle, but in modern times, obesity gradually began to increase in Asians as the eating habits changed and the living environment changed due to the influence of western culture.

In addition, in modern times, physical strength is reduced due to overwork, excessive drinking, stress, etc., which is a factor of stamina deterioration. In particular, due to mental stress, large amounts of calcium, magnesium, zinc, potassium, and protein are excreted through the urine, and potassium and protein are also reduced in the adrenal cortex cells that secrete stress-regulating hormones. This leads to abnormal hormone secretion, which lowers the stamina. Solutions for this include meals with plenty of energy and nutrition, plenty of rest and proper exercise.

Obesity is a state in which excess fat is accumulated in the body, and the cause of fat accumulation in the body is an excessive intake of sugar (carbohydrate) or excessive intake of fat.

The mechanism of reaching obesity is that by over-ingesting the sugar, the sugar in food is digested and becomes monosaccharide and absorbed into the body through the small intestine.Increase the blood sugar and insulin secreted by the stimulus acts on the fat cells to fat the sugar in the blood. It takes the cells and converts them into fat.

In addition, fat (triglyceride), which is the highest calorie among food ingredients, is broken down by pancreatic lipase and absorbed through the small intestine, and excess calories intake act to increase stored calories, resulting in increased stored calories. In other words, excess fat intake to reach obesity.

Therefore, studies on various anti-obesity agents are currently being conducted based on the idea of generating anti-obesity effects by inhibiting a part of certain pathways leading to obesity.

Cholesterol adsorption excretion, cholesterol underground, which inhibits the glycolysis digestion factor inhibition, blood sugar elevation suppression, or monosaccharide absorption inhibition or excess fat intake by inhibiting the obesity pathway by overingestion of glucose, cholesterol underground It is thought that obesity can be prevented and improved by the action, the blood triglyceride lowering action, or the lipase inhibitory action, and the research on the pharmaceutical component which has these actions is continuing.

First of all, the methods of inhibiting obesity include inhibiting glycolysis digestive enzymes, inhibiting monosaccharide absorption, and inhibiting blood sugar rise. These functions inhibit the path to obesity by ingesting sugar.

The glycolysis digestive enzyme is a digestive enzyme responsible for degrading disaccharides (sucrose, maltose, isomaltose, lactose, trehalose, etc.) to monosaccharides (glucose, galactose, etc.), and α-glucosidase, β -Glucosidase, sucrase, maltase, isomaltase, lactase, trehalase and the like. Glycolytic digestive enzyme inhibitors inhibit the glycolytic digestive enzyme responsible for the breakdown of disaccharides to monosaccharides, and delay the rapid rise in blood sugar after meals by delaying the digestion of sugars by oral ingestion. Because digestion is inhibited, decomposition into monosaccharide occurs slowly, so that absorption of monosaccharide into the intestinal tract is delayed and blood sugar rise is suppressed. For this reason, it is thought that fat synthesis from sugar falls and body fat accumulation is suppressed.

In addition, rapid postprandial blood sugar elevation and excessive insulin secretion due to excessive intake of carbohydrates (glucose) promote diabetes or hyperlipidemia in addition to obesity (pharmacology and treatment Vol. 19, No. 10 Oct. 274, 1991), glycolytic digestive enzymes Inhibition of diabetes mellitus or hyperlipidemia is also known to prevent and improve. Moreover, preventing hyperlipidemia is one of the effective methods of preventing atherosclerosis (latest medical metabolism, published by Biomedical Publishing Co., 1019, 1987).

Therefore, glycosylated digestive enzyme inhibitors, monosaccharide absorption inhibitors, or blood glucose elevation inhibitors are considered to be useful as antidiabetic agents, antihyperlipidemic agents, or antiarteriosclerosis agents.

Glycolytic digestive enzyme inhibitors currently used in medicine include acarbose (Acarbose, Bayer Pharmaceutical Co., Ltd.) and Bgriboose (AO-128: Daeda Pharmaceutical Co., Ltd.), a post-prandial hyperglycemic improver. In animal studies or clinical trials, the effect of suppressing postprandial blood sugar levels was confirmed, and the effectiveness of anti-obesity and anti-diabetic disease was also reported (Res. Exp. Med. Vol. 175, 87 (1979), Japanese Journal of Agricultural Chemistry vol. 63, 217 (1989), New Current vol. 6, 2 (1995).

In addition, fat (triglyceride) intake to inhibit the path to obesity, such as exclusion of cholic acid adsorption, cholesterol lowering, blood triglyceride lowering, lipase inhibition and the like.

Since fat (triglycerides) is broken down by pancreatic lipase and absorbed through the small intestine, inhibiting lipase is a useful action as an anti-obesity by lowering triglycerides in the blood. It is also useful as an antihyperlipidemic agent because serum lipids are lowered by inhibiting fat absorption into the intestinal tract.

Cholic acid (bile acid) adsorption excretion agent binds to cholic acid in the intestinal tract and increases excretion in excreta, inhibiting exogenous cholesterol absorption. In other words, in order to compensate for the reduction of cholic acid due to the increase in the amount of cholic acid excreted, the catabolism of cholesterol to cholic acid proceeds rapidly. It is thought that these actions lower blood cholesterol. Cholesterol catabolic excretion promotes cholesterol consumption and promotes fat breakdown, which is a raw material of cholesterol.

Drugs with cholic acid excretion include cholstyramine, a hypercholesterolemic drug, which is an anion exchange resin. By oral administration of the anion exchange resin, the anion exchange resin adsorbs cholic acid in the intestinal circulatory organs (Makino Isaora, Taisha, vol. 24, No. 8, 685-692, 1987). Fixing interferes with the resorption of cholic acid and promotes the conversion of cholesterol into cholic acid in the liver, resulting in a lowering of cholesterol levels in the blood. However, the usage of cholestyramine is to take 9 g in 100 mL of water, so a single dose is very large. There was a difficult problem.

As described above, a number of chemical synthetic compounds having respective actions have been reported and used as medicines, but they have a problem in that they have a high dose or are unpleasant at the time of taking them, and because they are chemically synthesized compounds, the test subjects have no effect on the human body at the time of administration. In some cases, safety was anxiety. In addition, the anti-obesity agent was mixed with food to prevent obesity in daily life, but it was not realized because it is a chemical synthetic compound and a large dose.

As a result, studies on natural substances derived from plants having blood glucose suppression activity or anti-obesity activity have been conducted. For example, baekryepi (나 白皮) and hydroxycitric acid, which is a component of garcinia, have recently been known. Nozirimycin, which is an active ingredient of, has a problem that its activity is too strong and unsuitable for mixing in food. In addition, it has been reported that extracts of guava leaves exhibited inhibitory activity against maltase and sucrase (Japanese Journal of Agricultural Chemistry, vol. 69, 339, 1995). There is a problem.

Therefore, there is a need for a study on a material that can enhance obesity as well as inhibiting obesity, including substances derived from natural substances.

In order to solve the problems of the prior art, an object of the present invention is to provide an anti-obesity composition that can suppress obesity.

Another object of the present invention is to provide a composition for enhancing stamina having a stamina enhancing activity.

In order to achieve the above object, the present invention provides a composition for inhibiting obesity, including an extract.

In another aspect, the present invention provides a composition for enhancing the stamina comprising the extract.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention, which can inhibit obesity and enhance stamina and research on various natural plants for the development of a material having excellent safety, confirmed that the extracts showed excellent effects on obesity inhibitory activity and stamina enhancer activity. Based on this, the present invention has been completed.

Shandedeok has been called as Samsam (뛰어난) because of its excellent efficacy compared to wild ginseng since ancient times, and it is called Ginseng (人蔘), Hyunsam (玄 蔘), Dansam (고) and Gosam (苦 蔘) It is treated as one of. This is recognized for its excellent efficacy in whales' oriental medicine such as <Shinnonbane herbaceous plant>, <Primary herbaceous tree>, <Kanyeobangbang>, and is widely used in folk medicine. There are many benefits of sanda duck, but especially saponin and inulin protect the gastrointestinal system, lungs and kidneys, and have great effects of expectoration, relieving, tonic, fever, dryness and detoxification. It contains a lot of essential linoleic acid, calcium, phosphorus, iron, etc. It is effective in maintaining bones and blood.

The sand is composed of 30.5% soluble solids, 0.42% total nitrogen, 2.63% crude protein, 0.395% water soluble protein, and 6.90% Brix value of sugar.

The present invention provides an anti-obesity and stamina enhancer composition comprising the extract.

The sandalwood extract is preferably included in 0.01 to 90% by weight, more preferably 0.1 to 80% by weight relative to the total weight of the composition for inhibiting obesity or stamina enhancer. When the extract contains less than 0.01% by weight, there is a problem that the effect of inhibiting obesity or stamina enhancement is insignificant, and when it exceeds 90% by weight, the increase in effect due to the addition amount is insignificant, which is inappropriate for economical use. There is a problem.

Sand Duck extract used in the present invention can be prepared by conventional methods. For example, the purified sand extract may be dried and pulverized, powdered, and then extracted by cooling for 5 days with about 10 times the amount of the extraction solvent, and the extraction stock extract may be prepared by a conventional method of filtration, concentration, and lyophilization. Extraction solvents that may be used include purified water, methanol, ethanol, propanol, butanol, gglycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, nucleic acid, diethyl ether, and dichloromethane. One or more solvents selected from the group consisting of the mixture can be mixed or extracted using one solvent, followed by solvent fractionation using one or two or more solvents.

In particular, the extract of the present invention is preferably extracted by repeatedly applying ultrasonic waves at regular intervals to the sand, or repeatedly applying ultrasonic waves and vacuum shock at regular intervals. same.

First, sand duck is mixed with water, and then it is subjected to a certain period of ultrasonic wave or ultrasonic wave and vacuum shock. Preparation of the mixture of the sand and water may be applied to conventional methods. In one example, the raw raw sand that is not dried can be washed with water as it is, and then mixed with water after being washed.In another example, the raw water is washed with water, dried, crushed by a grinder and mixed with water. It can also manufacture.

Preferably, the sand is washed with water and dried, and then pulverized in the form of No. 3 body with a pulverizer, and then mixed with water and then heated to homogenization at 60 ° C.

In addition, the method of applying ultrasonic waves to the mixture is repeatedly performed by applying ultrasonic waves for a predetermined time using an ultrasonic vibrator, and not applying ultrasonic waves for a predetermined time.

The adjustment of the time and the number of repetitions can be adjusted in consideration of economics and work efficiency, the time of applying the ultrasonic wave is preferably 2 to 15 minutes, the time of not applying the ultrasonic vibration is preferably 1 to 15 minutes, the number of repetitions is 1 To 10 times is preferred.

The frequency of the ultrasonic wave is preferably 15 to 40 kHz, and the amplitude (maximum / minimum displacement difference) of the ultrasonic wave is preferably 50 to 300 m. If the frequency is less than 15 kHz, the cavitation can be increased, but the penetration force is weakened. If the frequency exceeds 40 kHz, the penetration force is increased, but the cavitation is weakened. In addition, if the amplitude is less than 50 ㎛, both the cavitation and the penetration force cannot impose the kinetic energy required for the extraction of sand. If it exceeds 300 ㎛, the ultrasonic energy is overloaded and a special container must be used to maintain the ultrasonic generator. There is a problem.

In addition, the extraction temperature is 30 to 60 ℃, which is the temperature at which the maximum efficiency can be obtained in consideration of the stability of the saponin or irradiated saponin contained in the sand.

In general, a method of applying ultrasonic vibration to a target object is a method in which an ultrasonic horn of the ultrasonic vibrator contacts the diaphragm to apply indirect ultrasonic waves. However, the method of applying the ultrasonic vibration to the mixture in the extraction of the sandalwood extract of the present invention uses a method of applying the ultrasonic vibration in the state in which the ultrasonic horn of the ultrasonic vibrator is in direct contact with the mixture. The ultrasonic vibrator may use a conventional ultrasonic vibrator capable of satisfying the frequency and amplitude, but of course, the horn contacts a portion of the mixture, and the horn is made of titanium alloy so that the vibration is transmitted well. Good to do.

After the ultrasonic wave is added to the mixture at a predetermined cycle as described above, the sand extract is obtained by centrifuging the filtrate from which the extract component and the solid are separated, separating the supernatant, and then drying the supernatant. At this time, the solid may be removed by using a filter instead of the centrifugation process.

In ultrasonic extraction, it is possible to maximize the cavitation phenomenon by ultrasonic waves by efficiently removing bubbles (dissolved gas) generated during cavitation. The fine bubbles generated during the extraction diffuse into the bubbles under negative pressure, thereby reducing the vacuum of the bubbles, which in turn causes a decrease in the impact when the bubbles explode. Therefore, by removing the air bubbles present in the sand through the vacuum shock and at the same time to penetrate water into the fine structure of the sand cells to contact with the solid extract, applying an ultrasonic shock more efficiently than using only ultrasonic waves Sanda extract can be extracted.

Schematic process diagram of the extraction method using the ultrasonic and vacuum shock at the same time as shown in FIG.

In FIG. 2, the ultrasonic vibrator is the same as the ultrasonic vibrator used in the method of extracting the ultrasonic vibration of the predetermined period by applying the mixture.

The vacuum shock may be selectively performed before or after the ultrasonic vibration is applied to the mixture, and the vacuum and atmospheric pressure are repeatedly applied to the mixture of sand and water, which are homogenized repeatedly at a predetermined cycle.

The vacuum device used for the vacuum shock can be used as a general vacuum device, of course, in this case, the vacuum is applied to the vacuum for 1 to 15 minutes in consideration of the economic efficiency and extraction efficiency in the step of not applying ultrasonic vibration, atmospheric pressure It is preferable to keep 1 to 15 minutes.

More preferably, the mixture of homogenized sand and water is subjected to vacuum for 1 to 15 minutes, the vacuum is released, and then the ultrasonic vibration is applied at normal pressure for 2 to 15 minutes at repeated 2 to 7 times. Even in this case, it is good to be made at a temperature condition of 30 to 60 ℃ over the entire step.

After applying a vacuum shock and ultrasonic waves to the mixture at a constant cycle as described above, the sand mixture is obtained by separating the extract component and the solid, centrifugation of the filtrate to separate the supernatant, and drying the supernatant.

Extraction method of the above-mentioned Shandeok extract can significantly shorten the time required for the conventional extraction method (complete extraction within 20 minutes ~ 3 hours), the extraction temperature is less than 65 ℃ that saponin destruction and alteration occurs There is an effect that can extract a high purity sandalwood extract in a short time without destruction or alteration of the extract extracted from sandalwood at 30 ~ 60 ℃. In addition, it is possible to produce sandalwood extracts containing 11% to 12% more irradiated than the water content (8% to 9%) of the conventional method of extracting water, as well as high yields (more than 30%). There is an effect that can produce. In addition, the extraction method of the extract of Shandeok, which repeatedly applies ultrasonic waves and vacuum shocks at a predetermined cycle to the mixed solution of Sandak and water, shows a higher extraction efficiency (40% or more) than using only ultrasonic waves of a certain period.

The anti-obesity and stamina enhancer composition comprising the extract may be used as a drug, drug additives, health functional food, food, food additives, beverages, or beverage additives, and for this purpose, powders, capsules, tablets, granules, powders It may be formulated in the form of liquid, beverage, porridge, and the like.

In the case of using the composition for inhibiting obesity or stamina enhancer comprising the extract of the present invention as a medicine, the form of powder, granules, tablets, capsules, pills, troche, liquid solution, suspension, emulsion, and Oral preparations, such as a syrup, can be used, These can be used individually or in combination, respectively according to a symptom. These various formulations are conventionally known carriers commonly used in the field of pharmaceutical formulations, such as excipients, binders, preservatives, oxidative stabilizers, disintegrants, lubricants, copulation agents, etc. according to the purpose in a commercial manner. It can be formulated using. In addition, when preparing a medicament of the present invention, it can be used in combination with other plant extracts having an obesity inhibitory effect, such as laurel, guave leaves, wheat, oolong tea, garcinia, Gymnema sylvestre extract.

Carriers that can be used can be used without any particular limitation depending on the formulation, preferred is solid carriers such as starch, lactose, mannitol, carboxymethylcellulose, corn starch, and inorganic salts, distilled water, saline solution, aqueous glucose solution, ethanol Liquid carriers such as alcohols such as alcohol, propylene glycol, and polyethylene glycol, and oily carriers such as various animal and vegetable oils, white petrolatum, paraffin, and waxes.

In addition, the composition for inhibiting obesity or stamina enhancer including the extract of the present invention may be prepared as a food by mixing with the composition itself, or various components used in the conventional food. In addition, when preparing the food of the present invention can be used in combination with plant extracts having an obesity inhibitory effect, such as laurel, guave leaves, wheat, oolong tea, garcinia, Gymnema sylvestre extract.

As the form of the food to be prepared, it can be prepared in all kinds of foods such as solid foods, creamy or jam-like semi-liquid foods, gel foods, beverages, etc. Any substrate can be used to prepare soft drinks, juices, coffee, tea, liqueurs, milk, whey beverages, lactic acid bacteria beverages, candies, chewing gum, chocolate, gummi, yogurt, ice cream, puddings, water yolks and the like.

Various ingredients can be used to prepare the above foods, and for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubus side, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, Lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium bilisobate, glycerol, propylene glycol, glycerol fatty acid ester, polyglycerol fatty acid ester, fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan And casein, gelatin, pectin, agar, vitamin B, nicotinic acid amide, pantothenic acid calcium, amino acids, calcium salts, pigments, flavorings, preservatives and the like, which can be used as conventional food raw materials can be prepared by appropriately blending.

The anti-obesity or stamina enhancer composition comprising the extract of the present invention is readily available in everyday life and is inexpensive because it is inexpensive, and does not cause the side effects of the obesity inhibitors dependent on conventional medicines, and causes obesity. Inhibits and has a noticeable effect on stamina enhancement. In particular, by inhibiting obesity there is an effect that can prevent the occurrence of diabetes mellitus, arteriosclerosis, hypertension, heart disease, gallstones, gout, kidney disease caused by obesity.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

EXAMPLE

Example 1 Preparation of Shandeok Extract

The powder was dried, ground and powdered, and then poured into 10 times of purified water, and extracted by chilling for 5 days at about 20 ° C., and then extracting the extract was filtered through a 300 mesh filter cloth. The mixture was left at 10 ° C. for 10 days, aged at low temperature, filtered, concentrated under reduced pressure at 60 ° C., and dried to prepare an extract.

Example 2

The sand was washed with water, dried, and then pulverized into Pharmacopoeia No. 3 sieve. 300 mL of water and 20 g of the ground sand were mixed and mixed, followed by homogenization by heating at 60 ° C.

The mixture of sand and water was subjected to ultrasonic waves for 11 minutes at a vibrator vibration output of 20,000 Hz and a total output of 80%. The ultrasonic vibrator was ULW 700H (manufactured by Ulsso Hitech). The extract extracted from the ultrasonic vibrator was filtered using a filter, the filtrate was centrifuged, and the supernatant subjected to centrifugation was lyophilized to prepare an extract. The temperature was maintained at 40 ~ 50 ℃ during the extraction process.

Example 3

The sand was washed with water, dried, and then pulverized into Pharmacopoeia No. 3 sieve. 300 mL of water and 20 g of the pulverized sandder were mixed in a 500 mL beaker, followed by homogenization by heating at 60 ° C.

After the homogenized mixture was placed in an O-ring-type cocker beaker (PYREX, LG Chemical Co., Ltd.), the reactor was vacuumed for 11 minutes while maintaining the water bath temperature at 45 ° C., After decompressing and changing to normal pressure, ultrasonic waves were applied for 11 minutes using the ultrasonic vibrator used in Example 1. After the ultrasonic wave was applied again, the vacuum device was depressurized and vacuum was applied for 11 minutes. The pressure was released to normal pressure, and the ultrasonic wave was applied for 11 minutes. The temperature was maintained at 40 ~ 50 ℃ during the extraction process.

The extract extracted above was filtered using a filter, the filtrate was centrifuged, and the supernatant obtained by centrifugation was dried to prepare an extract.

Example 4. Obesity Inhibitory Effect (Animal Experiment)

In order to measure the obesity inhibitory effect of the extracts prepared in Example 3, 45 rats of Sprague-Dawley (SD) rats of 4 weeks of age were purchased and pellet pellets were marketed for 1 week. After salary, five animals were divided into nine groups. The normal control group (group 3) continued to feed only pellets, while the remaining group 6 fed a pelleter diet and a cafeteria diet known to cause obesity closest to the most common types of obesity in humans (Nutr. Biochem). 6, 151 (1995)), all feed and water were freely ingested. After feeding the cafeteria diet for 2 weeks, 3 g of the 6 groups fed the cafeteria diet was diluted to 10% by weight in physiological saline on the basis of the extract from Example 3. , 1 g / kg and 10 g / kg were administered orally twice a day, and normal saline and obese controls were orally administered with the same volume of saline.

After oral administration of the sample for 6 weeks, liver and abdominal lipids were extracted, and the triglyceride and abdominal fat of liver were quantified by enzyme method, and the results are shown in Table 1 below.

division Normal control Obesity Control Shandeok Extract Administration Group 0.01 g / kg administration Weight gain (g) 221 ± 5 295 ± 5 258 ± 5 Abdominal fat (g) 8.01 ± 0.31 12.68 ± 0.32 9.55 ± 0.35 Liver triglycerides (mg / g) 62.4 ± 2.0 86.6 ± 3.0 72.4 ± 3.9 1 g / kg administration Weight gain (g) 225 ± 5 300 ± 5 272 ± 5 Abdominal fat (g) 8.44 ± 0.24 16.82 ± 0.31 12.51 ± 0.35 Liver triglycerides (mg / g) 68.6 ± 2.2 90.1 ± 3.0 76.4 ± 3.0 10 g / kg administration Weight gain (g) 227 ± 4 307 ± 5 276 ± 5 Abdominal fat (g) 8.57 ± 0.29 18.42 ± 0.33 13.75 ± 0.35 Liver triglycerides (mg / g) 70.0 ± 2.1 88.8 ± 3.0 77.1 ± 3.9 Note 1. Abdominal fat measurement: Remove all abdominal subcutaneous fat, remove blood with physiological saline, and then weigh it. 2. Hepatic triglyceride measurement: total by Folch method (Wako Pure Chemical Ind. Ltd., Japan) Determination by enzyme method after lipid extraction

Through the above Table 1, the experimental group administered the Sandodeok extract of the present invention inhibited weight gain, abdominal fat accumulation, and hepatic triglyceride accumulation, and showed a weight inhibitory effect compared to the obese control group who gained weight by the cafeteria diet. In addition, abdominal fat, and liver triglyceride content was confirmed that the reduction effect is remarkable. In addition, all of the groups of extracts did not show a particular abnormality compared to the control group, it can be seen that there is no problem in safety at all.

Example 5. Obesity Inhibitory Effect (Clinical Experiment)

After dilution of the sandalwood extract prepared in Example 3 to 50% by weight of water before breakfast and lunch to each adult male and female of obesity degree 25% or more calculated by Equation 1 below, based on the sandalwood extract 8 g each and 50 g at dinner. After 4 weeks of ingestion, body, clinical, and biochemical measurements were compared with those before ingestion, and the results are shown in Table 2 below.

[Equation 1]

division Men (average 10 people) Women (average 10) Before intake Before intake After ingestion After ingestion Weight (kg) 78.2 ± 9.2 73.3 ± 8.3 64.9 ± 7.2 59.8 ± 6.3 Body fat (%) 27.2 ± 4.1 25.9 ± 5.1 33.2 ± 3.9 30.9 ± 4.1 Waist circumference (inch) 93.2 ± 3.1 90.1 ± 2.9 85.1 ± 2.9 82.9 ± 3.1 Hip circumference (inch) 92.1 ± 4.1 90.3 ± 3.7 87.2 ± 3.8 85.1 ± 2.9 Systolic Blood Pressure (mmHg) 142.3 ± 6.1 138.1 ± 5.9 100.1 ± 7.7 137.3 ± 7.2 Diastolic blood pressure (mmHg) 90.2 ± 4.1 87.4 ± 3.8 91.1 ± 4.3 87.7 ± 4.0 Fasting Blood Sugar (mg / dl) 12.9 ± 7.1 123.1 ± 6.8 126.1 ± 7.1 124.1 ± 7.0 Blood Triglycerides (mg / ㎗) 197.1 ± 17.1 188.3 ± 16.3 193.2 ± 16.9 184.2 ± 15.2 Blood total cholesterol (mg / ㎎) 237.2 ± 19.2 229.3 ± 17.1 238.2 ± 17.3 228.1 ± 16.1 HDL-cholesterol (mg / dl) 40.3 ± 0.2 40.2 ± 8.1 46.2 ± 9.1 45.3 ± 8.7 LDL-cholesterol (mg / dl) 141.1 ± 7.1 136.3 ± 7.0 142.3 ± 6.8 135.2 ± 6.1

Through Table 2, as a result of ingesting the extract of the present invention, it was confirmed that the weight of the average man is about 4.9 kg, the woman is reduced by 5.1 kg compared to before ingestion. In addition, waist circumference, hip circumference, and blood total cholesterol and LDL-cholesterol concentrations were significantly decreased compared to before consumption. Through the above results, it was found that the extract of the present invention can effectively inhibit human obesity.

Example 6 Stamina Enhancer Effect

In order to evaluate the effect of the extracts on the stamina enhancement of rats, 30 male ICR rats weighing 21-27 g were divided into 6 groups of 5 rats each, and the extract of Example 3 and the carboxymethyl cellulose of CMC ) Was dosed at 0.5 mg / kg, 50 mg / kg and 5 g / mg, respectively, once orally in each group for 7 days, and then the swimming time was measured on the 8th day. The test method used a slight modification of Richard's swimming test method (Richard. H. B. et al., Am. J. Physiol., 168, 178, 1963).

The pool was 25 × 40 × 13 cm in width × length × depth and the water temperature was maintained at 25 ° C. In order to shorten the swimming time and to reduce the differences between individuals, the rats were swimming for 1 minute in the pool containing the surfactant (trio, aekyung industry) at a concentration of 0.2% in the morning of the test day, and immediately transferred to the ordinary pool containing only tap water. Swim for a minute to remove fat from the hair of the mouse. After about 3 hours of physical recovery and hair drying period, the rats were weighed and the test substance was administered. Test substance was homogeneously suspended in 0.5% CMC-Na aqueous solution and orally administered to rats at a volume of 0.1 mL per 10 g, and in a pool containing 0.01% of surfactant (trio) at 1 hour after administration of the test substance. The swimming time of the mice was measured. The results are shown in Table 3 below.

division Swimming time (minutes) 0.5 mg / kg Shandeok Extract Administration 124.2 ± 11.8 CMC dosing 85.1 ± 4.4 50 mg / kg Shandeok Extract Administration 141.1 ± 15.0 CMC dosing 95.1 ± 5.7 5 g / kg Shandeok Extract Administration 150.5 ± 9.5 CMC dosing 96.2 ± 3.7

Through Table 3, it was confirmed that the swimming time of the rat in the group administered with the extract of the present invention was significantly increased compared to the control group administered the CMC.

Example 7. Tablet Preparation

Tablets (200 mg each) were prepared by tableting the extracts prepared in Example 3 according to a conventional method.

Example 8 Preparation of Granules

The tablets prepared in Example 7 were powdered, granulated, and sieved to prepare granules.

Example 9 Candy Preparation

Candies were prepared in a conventional manner by mixing the extract of Shandeok, granulated sugar, syrup, citric acid, fragrance, and pigment separated in Example 3.

Example 10 Beverage Preparation

The beverage was prepared by mixing 100 mL of water in 4 g of the extract from Example 3, which was prepared in Example.

Sandodeok extract of the present invention is derived from natural plants and does not cause side effects of the drug in the obesity inhibitors dependent on conventional medicines are safe for the human body, readily available in daily life and inexpensive because it is inexpensive, and also inhibits obesity It has a remarkable effect on enhancing stamina. In addition, by inhibiting obesity there is an effect that can prevent the occurrence of diabetes, arteriosclerosis, hypertension, heart disease, gallstones, gout, kidney disease caused by obesity.

Claims (7)

A composition for inhibiting obesity comprising sandalwood extract, which is an extract component obtained from the supernatant after dispersing codonopsis lancelolata in water and repeatedly applying ultrasonic waves at regular intervals. The method of claim 1, The extract for inhibiting obesity is included in 0.01 to 90% by weight relative to the total weight of the composition. The method of claim 1, The ultrasonic wave is a composition for inhibiting obesity that is performed by applying a frequency of 15 to 40 kHz at 30 to 60 ℃. The method of claim 1, In addition, the composition for inhibiting obesity prepared by a method comprising the step of repeatedly applying a vacuum shock when ultrasonic application. The method of claim 1, The composition is a composition for inhibiting obesity is one kind selected from the group consisting of health food, food, food additives, beverages, and beverage additives. delete delete

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