KR100471986B1 - Cosmetic compositions effective for Propionibacterium acnes - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract extracted from grapefruit seeds, red ginseng, nimbus fruit, the cosmetic composition of the present invention has an antibacterial action.

Description

Cosmetic composition for preventing acne {Cosmetic compositions effective for Propionibacterium acnes}

The present invention relates to a cosmetic composition containing a mixed extract extracted from grapefruit seeds, gosam, nimbus fruit, and more particularly, the effect of treating or preventing skin diseases such as acne by a stronger antibacterial action of the extract. It relates to a cosmetic composition having.

There are various microorganisms on the skin. These microorganisms cause various skin diseases. Staphylococcus aureus, which causes purulent skin, includes Staphylococcus epidermis, Acne bacteria (Propionibacterium acnes), Oily skin infections (oval patirosporum bacteria, Pity ovale) These bacteria decompose sebum or sweat secreted on the skin and produce odors. In addition, the degradation products irritate and irritate the skin. In particular, acne bacteria (Propionibacterium acnes) breaks down sebum by using a fatty acid degrading enzyme lipase (lipase) to produce free fatty acids. These free fatty acids not only irritate the skin but also cause inflammatory lesions such as papules, pustules and nodules, which are red rashes found in acne.

Dermatophytes invade the stratum corneum of the hair and skin, causing superficial mycosis. The skin filaments that cause superficial mycosis include Microsporum, Trichophyton, and Epidermophyton, and fungi represent ringworm. Candida albicans causes cutaneous candidiasis and chronic mucosal candidiasis. These fungi represent superficial mycosis, in which the fungus parasitic in the keratinous tissue of the skin and multiplies and causes lesions. Another cause of granulomatous lesions in skin and subcutaneous tissue is Sporotrix schenckii.

Many herbal medicines are known for their use in skin diseases caused by detergents and medicinal herbs. The research to scientifically prove the effectiveness of conventional herbal medicines used and handed down conventionally, and to find herbal ingredients with new antimicrobial activity has been actively conducted.

The most well-known antibacterial medicines are berberine, alkaloids contained in Phellodendron amurense and Coptis japonica, and are listed as berberine hydrochloride in Korea. Widely used in diseases, strong antimicrobial activity against acne progeny (propionibacterium acnes) has been reported.

Red ginseng roots and outposts have been used as antidote in the private sector, and in oriental medicine, it has been used for dry stomach, tonic, anti-inflammatory, analgesic, fever and diuresis.

2 ', 4', 7-trihydroxy-5-methoxy-8- (5-hydroxy-5-methyl-2-isopropenylhexyl) flavone, a red ginseng flavonoid component, is used as an antiulcer treatment . Flavonoids from the roots of Ginseng also show excellent antibacterial activity against Staphylococcus aureus and Acne.

Alkaloids of the matrine family are reported to exhibit strong insecticidal and antimicrobial activity.

In addition, the makiain component is 12.5-25 mu g / ml at concentrations of skin ringworm (a bacterium that causes ringworm; Trichophyton mentagrrophytis, T. tonsurans, Epidermophyton floccosum) It has been reported to exhibit excellent antimicrobial activity against microsporum gypseum.

In particular, ginseng extract has been traditionally used for the treatment of skin eczema and neurodermatitis because it has an inhibitory effect on skin fungi. It has also been reported to be used for alopecia. The root of ginseng contains a lot of lupine alkaloids. These include (+)-martrin, (+)-oxymatrin, (+)-isomatrine, (+)-sophoranol, and angarin, and isoan hydroicaritin as a flavonoid type. It contains many types of flavonoids, such as, kurarinone, kuraridin, kuraridol, kurariol, kurarinol, and neokurarinol. In addition, 1-maakiain was also identified in the root of red ginseng.

Grapefruit of the present invention is a fruit of the grapefruit tree belonging to the citrus (Citrus). The origin of this variety is considered Jamaica of the West Indies. It smells like grapes and runs like a bunch of grapes. It is round in the shape of orange and has a diameter of 10-15cm. The outer shell is leather and the surface is smooth and yellow. The cuticle is thin and soft. The pulp is pale yellow, rich in juice, tastes sour, sweet and bitter. Recently, varieties with pink pulp have been developed (Doosan World Encyclopedia).

Accordingly, it is an object of the present invention to provide a natural extract having the effect of treating or preventing skin diseases such as acne with a stronger antibacterial action.

Another object of the present invention is to provide a cosmetic composition comprising an extract having such efficacy.

The object of the present invention is achieved by an extract extracted from grapefruit seeds, red ginseng, nimbus fruit and a cosmetic composition containing the same.

The present invention will be described in more detail as follows. A group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, ethylene glycol, and propylene glycol as extracting solvents on dry weight after complete drying of grapefruit seeds, red ginseng and nim fruit Add 1-20 fold volume of one or more solvents selected from. Extraction method is a method of extracting by heating at 50-95 ° C for 4-20 hours or extracting active ingredient at 5-37 ° C for 1-15 days while cooling condenser is installed to prevent evaporation of solvent. Can be. The extract thus extracted is concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser, and then added to the cosmetic composition in an amount of 0.001-10%, preferably 0.001-5% as its dry weight. In other words, it is added to softening cream (skin), nutrition cream (lotion), nutrition cream, massage cream, essence, pack, emulsified foundation, and the like. The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example 1.

0.5 kg of completely dried grapefruit seeds, 0.5 kg of red ginseng and 0.5 kg of Nimbus fruit were added to 20 kg of water, and extracted by boiling in an extractor equipped with a cooling condenser for 5 hours. After aging for one day, the resultant was filtered with Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 105 g (dry weight).

Example 2.

Completely dry 0.5 kg grapefruit seed, 0.5 kg red ginseng, and 0.5 kg neem fruit, and put it in 20 kg of 50% ethanol, boil for 5 hours in an extractor equipped with a cooling condenser, filter with 400 mesh filter cloth, and cool to room temperature. After aging at room temperature for 7 days, the resultant was filtered with Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 102 g (dry weight).

Example 3.

0.5 kg of grapefruit seeds, 0.5 kg of red ginseng and 0.5 kg of Neem fruit were washed with dry water, dried, and boiled in 10 l of 80% ethanol, extracted by boiling in an extractor equipped with a cooling condenser for 5 hours, and filtered through a 400 mesh filter cloth. After aging for 7 days, the resultant was filtered with Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 98 g (dry weight).

Example 4.

0.5 kg grapefruit seed, 0.5 kg red ginseng and 0.5 kg neem fruit were washed with dry water, dried, and poured into 20 l of ethanol, boiled for 5 hours in an extractor equipped with a cooling condenser, filtered with 400 mesh filter cloth, and filtered at 5-15 ° C. for 7 days. After aging, it is filtered with Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 122 g (dry weight).

Example 5.

After washing 0.5kg grapefruit seed, 0.5kg red ginseng and 0.5kg neem fruit with clean water, drying it, put it in 20ℓ of ethanol, extract it for 15 days at 15-37 ℃, filter it with 400 mesh filter cloth, and leave it at 5-15 ℃ for 7 days. After aging chicken was filtered with Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 118 g (dry weight).

Example 6-20.

Using the solvent of Table 1 below, the extraction was carried out in the same manner as in Example 5, and the results are shown in Table 1 below.

Table 1. Experimental Results of Example 6-21

              Dry Weight of Extraction Solvent Final Extraction

                                               (Unit: g)

Example 6 50% Ethanol 100

Example 7 80% Ethanol 134

Example 8 Methanol 125

Example 9 50% Methanol 105

Example 10 80% Methanol 135

Example 11 n-propanol 120

Example 12 Isopropane 101

Example 13 n-butanol 121

Example 14 Hydrous Glycer 76

Example 15 Hydrous propylene glycol 86

Example 16 Hydrated Butylene Glycol 99

Example 17 Benzene 54

Example 18 Chloroform 42

Example 19 Hexane 92

Example 20 Acetone 88

Example 21 Purified Water 111

Experimental Example 1.

The antibacterial effect of the grapefruit seed, Gosam and Neem fruit mixture extract of the extract 1-21 obtained in Example 1-21 was measured as follows.

After incubating each strain for 1 day, 200 µl was plated in a petri dish, 1 g of each extract was dissolved in 9 ml of 50% 1, 3-butylene glycol aqueous solution, and 30 µl of the mixed solution was taken. It was incubated in the following conditions by dropping the paper disc (paper disc) in which the strain is present (Table 2).

Table 2. Culture conditions of strains for measuring the antimicrobial effect of natural extracts according to the present invention

number Strains Medium Time, temperature type One Candida albicans (ATCC 10259) Nutrient agar 48 hours, 37 ℃ Exhalation 2 Escherichia coli (ATCC 25922) Tryticase soy agar 48hrs, 37 ℃ Exhalation 3 Streptococcus aureus (Staphylococcus aureus ATCC 29737) Nutrient agar 48 hours, 37 ℃ Exhalation 4 Bacillus subtilis ATCC 6633 Nutrient agar 48 hours, 37 ℃ Exhalation 5 Streptococcus mutans ATCC 25175 Brain heart infusion 24 hours, 37 ℃ Exhalation 6 Staphylococcus epidermidis ATCC 12228 Nutrient agar 48 hours, 27 ℃ Exhalation 7 Acne Bacteria (Propionibacterium acne ATCC 6919) Reinforced clostridial agar medium 72 hours, 37 ℃ anaerobe

The results are shown in Table 3 to measure the diameter of the clear zone appearing around the paper disc (paper disc). As a control, the extract prepared by extracting the grapefruit seeds, gosam and nim fruit of 1.5 kg each by dry weight was used. Each is described as -1, -2, -3 after the number of the examples. The results are shown in Tables 4, 5, and 6 below.

Table 3. Antibacterial Effects of Grapefruit Seed, Gosam and Neem Fruit Extract

        (Diameter of clean area: mm)

number sample Use strain (number of table 1) One 2 3 4 5 6 7 One Example 1 21 32 24 24 28 21 25 2 Example 2 19 21 24 22 29 21 25 3 Example 3 19 23 23 22 25 22 26 4 Example 4 18 28 23 22 25 22 26 5 Example 5 19 25 25 23 22 22 26 6 Example 6 21 28 29 23 21 29 28 7 Example 7 22 26 28 23 21 28 28 8 Example 8 21 25 24 25 21 28 29 9 Example 9 21 21 21 28 25 27 26 10 Example 10 22 22 25 21 26 27 26 11 Example 11 21 23 25 25 28 25 22 12 Example 12 18 24 26 26 28 26 25 13 Example 13 16 24 26 26 29 24 23 14 Example 14 16 25 24 25 25 25 24 15 Example 15 15 25 24 23 26 28 24 16 Example 16 16 26 23 24 24 28 24 17 Example 17 18 26 25 21 21 28 25 18 Example 18 19 28 23 22 21 21 25 19 Example 19 21 24 26 21 26 25 22 20 Example 20 18 21 28 21 26 29 21 21 Example 21 31 21 28 25 28 29 22

Table 4. Antimicrobial Effects of Grapefruit Seed Extract

             (Diameter of clean area: mm)

number sample Use strain (number of table 1) One 2 3 4 5 6 7 One Example 1-1 18 17 18 15 16 15 18 2 Example 2-1 15 15 18 15 15 16 15 3 Example 3-1 16 15 15 12 16 16 16 4 Example 4-1 16 15 15 16 17 15 18 5 Example 5-1 18 16 16 16 18 18 18 6 Example 6-1 19 16 16 16 15 15 18 7 Example 7-1 18 15 16 15 18 16 15 8 Example 8-1 13 17 18 15 19 16 15 9 Example 9-1 15 17 17 18 19 16 16 10 Example 10-1 11 18 17 18 16 18 16 11 Example 11-1 11 19 18 18 16 18 15 12 Example 12-1 16 15 18 15 15 18 13 13 Example 13-1 16 15 17 15 14 15 15 14 Example 14-1 15 16 17 16 11 16 15 15 Example 15-1 18 13 14 18 13 16 15 16 Example 16-1 12 15 14 15 12 18 16 17 Example 17-1 13 18 14 18 12 18 18 18 Example 18-1 18 18 15 18 12 15 18 19 Example 19-1 19 15 12 15 13 15 19 20 Example 20-1 15 15 13 16 15 16 19 21 Example 21-1 18 15 13 14 16 16 15

Table 5. Antibacterial Effect of High Ginseng Extract

      (Diameter of clean area: mm)

number sample Use strain (number of table 1) One 2 3 4 5 6 7 One Example 1-2 12 12 15 11 12 11 15 2 Example 2-2 13 13 16 12 12 15 12 3 Example 3-2 15 12 13 12 12 15 12 4 Example 4-2 12 15 12 13 13 12 13 5 Example 5-2 13 15 13 15 13 13 12 6 Example 6-2 15 15 12 15 15 12 12 7 Example 7-2 11 13 15 15 15 12 12 8 Example 8-2 12 13 15 12 16 13 15 9 Example 9-2 13 12 15 13 15 12 15 10 Example 10-2 15 12 12 13 15 12 15 11 Example 11-2 12 15 13 13 12 13 13 12 Example 12-2 13 15 12 13 11 16 13 13 Example 13-2 15 16 15 12 11 16 13 14 Example 14-2 13 15 12 11 11 12 12 15 Example 15-2 11 15 15 12 12 15 13 16 Example 16-2 12 12 11 13 12 14 12 17 Example 17-2 13 13 13 13 12 12 15 18 Example 18-2 15 12 13 15 12 11 12 19 Example 19-2 16 13 13 16 13 12 12 20 Example 20-2 16 15 15 16 12 13 12 21 Example 21-2 18 15 12 16 12 13 15

Table 6. Antimicrobial Effects of Neem Fruit Extract

         (Diameter of clean area: mm)

number sample Use strain (number of table 1) One 2 3 4 5 6 7 One Example 1-3 18 15 18 15 15 15 12 2 Example 2-3 16 15 16 15 15 12 15 3 Example 3-3 18 15 15 15 12 13 15 4 Example 4-3 18 18 18 16 15 12 16 5 Example 5-3 15 18 15 18 18 12 15 6 Example 6-3 14 15 16 18 15 13 15 7 Example 7-3 15 16 18 15 13 13 18 8 Example 8-3 16 18 14 16 12 12 15 9 Example 9-3 18 13 15 16 15 12 18 10 Example 10-3 18 12 14 14 15 15 15 11 Example 11-3 18 15 15 12 15 12 16 12 Example 12-3 15 18 15 13 16 12 15 13 Example 13-3 17 15 14 13 14 13 16 14 Example 14-3 18 15 16 15 13 12 14 15 Example 15-3 18 15 16 12 13 12 14 16 Example 16-3 19 14 18 12 12 13 14 17 Example 17-3 15 16 15 12 12 15 13 18 Example 18-3 15 16 14 13 15 12 17 19 Example 19-3 14 13 15 15 15 12 15 20 Example 20-3 12 13 14 15 12 15 17 21 Example 21-3 18 13 15 13 13 16 14

Using the raw material prepared in the above Example was prepared in the same manner as in the formulation example below.

Prescription Example 1.

A prescription example of a skin lotion (skin lotion) in cosmetics containing grapefruit seeds, a mixture of goose and nim fruit is as follows. Here, grapefruit seed and the ginseng mixed extract is that of Example 2.

Table 7. Prescription example of skin lotion (skin lotion) according to the present invention

number Raw material Formulation Example 1 (%) Comparative Formulation Example 1 (%) One  Grapefruit Seed, Red Ginseng and Neem Fruit Mix Extract (Example 2) 10.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (60) Cured Castor Oil 1.00 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 1: Raw material No. 11, raw materials 2, 3, 4, and 8 were sequentially added and stirred to dissolve, followed by heating and dissolving raw material No. 5 at about 60 ° C., followed by stirring with raw material No. 10 Input to raw material No. 11. Finally, the raw materials 1, 6, 7, and 9 were added and sufficiently stirred before passing through a microfluidizer and aged.

Comparative Formulation Example 1: Raw material No. 11, raw materials 2, 3, 4, and 8 were sequentially added and stirred to dissolve. Then, raw material No. 5 was heated and dissolved about 60 ° C., and raw material No. 10 was added and stirred. To 11 the raw material. Finally, the raw materials 6, 7, and 9 are added and sufficiently stirred before passing through a microfluidizer and aged.

Prescription Example 2.

A prescription example of nutritional lotion in cosmetics containing grapefruit seeds, a mixture of Gosam and Neem fruit is as follows. Here, grapefruit seed, goose and nim fruit mixture extract are those of Example 2.

Table 8. Prescription example of nutrition lotion according to the present invention

number Raw material Formulation Example 2 (%) Comparative Formulation Example 2 (%) One   Grapefruit Seed, Red Ginseng and Neem Fruit Mix Extract (Example 2) 10.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 10.0 10.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.3 0.3 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 2: Raw materials 2, 3, 4, 5, and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1, 7, 8, and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Pass it through again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Comparative Formulation Example 2 The raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipids and the raw materials 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature, and then again added to the microfluidizer. Pass it again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Prescription Example 3

Prescription examples of nutritional creams in cosmetics containing grapefruit seeds, extracts of Gosam and Neem fruit are as follows. Here, grapefruit seed, goose and nim fruit mixture extract are those of Example 2.

Table 9. Prescription example of nutrition cream according to the present invention

number Raw material Formulation Example 3 (%) Comparative Formulation Example 3 (%) One Grapefruit Seed, Red Ginseng and Neem Fruit Mix Extract (Example 2) 10.0 - 2 Cytostolol 4.0 4.0 3 Polyglyceryl 2-oleate 3.0 3.0 4 Ceramide 0.7 0.7 5 Ceteares-4 2.0 2.0 6 cholesterol 3.0 3.0 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 22.0 22.0 10 Carboxy Vinyl Polymer 0.5 0.5 11 Triethanolamine 0.5 0.5 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 3: The raw materials 2, 3, 4, 5, and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1, 7, 8, and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Pass it through again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Comparative Formulation Example 3 The raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipids and the raw materials 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature, and then again added to the microfluidizer. Pass it again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Prescription Example 4.

A prescription example of a massage cream among cosmetics containing grapefruit seeds, a mixture of Gosam and Nimbus fruits is as follows. Here, grapefruit seed, goose and nim fruit mixture extract are those of Example 2.

Table 10. Prescription examples of massage cream according to the present invention

number Raw material Formulation Example 4 (%) Comparative Formulation Example 4 (%) One Grapefruit Seed, Red Ginseng and Neem Fruit Mix Extract (Example 2) 10.0 - 2 Beeswax 10.0 10.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 40.0 6 Squalane 5.0 5.0 7 Caprylic / Capric Triglycerides 4.0 4.0 8 Carboxy Vinyl Polymer 0.2 0.2 9 glycerin 5.0 5.0 10 Butylene glycol 3.0 3.0 11 Propylene glycol 3.0 3.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 4 The raw materials 8, 9, 10, 11, 13, 16 were heated to 80-85 ° C. with mixing and stirring, and then fed into the manufacturing unit, followed by the operation of an emulsifier, and the raw materials 2, 3, 4, 5, 6, 7, 12 is heated to between 80-85 ° C and emulsified. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add raw material No. 14, cool to 45 ℃, add raw material No. 15, and cool to 35 ℃. It is cooled to 25 ° C. and aged.

Comparative Formulation Example 4 The raw materials 8, 9, 10, 11, 13, and 16 were mixed and stirred to be heated to 80-85 ° C. and fed into the manufacturing unit, followed by the operation of an emulsifier, and the raw materials 2, 3, 4, 5, 6 , 7, 12 were heated to 80-85 ° C. and then emulsified. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, and the raw material No. 14 is added and cooled to 45 ° C., then the raw material No. 15 is added, cooled to 35 ° C., and aged.

Prescription Example 5.

Prescription examples of nutritional essences in cosmetics containing grapefruit seeds, mixed extracts of ginseng and neem fruit are as follows. Here, grapefruit seed and the ginseng mixed extract is that of Example 2.

Table 11.Prescription Examples of Nutritional Essences According to the Present Invention

number Raw material Formulation Example 5 (%) Comparative Formulation Example 5 (%) One Grapefruit Seed, Red Ginseng and Neem Fruit Mix Extract (Example 2) 10.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 15.0 15.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 5: The raw materials 2, 3, 4, 5, and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1, 7, 8, and 14 are mixed and homogenized at a constant temperature to pass through a microfluidizer, and then the raw material 9 is gradually added at a constant temperature to homogenize and then microfluda again. Pass it through again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Comparative Formulation 5: Raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipids and the raw materials 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature, and then again added to the microfluidizer. Pass it again. Then, the raw materials 10, 11, 12, 13 are added to disperse to stabilize and mature.

Test Example 1.

The antimicrobial effect of Formulation Example 1-5 and Comparative Formulation Example 1-5 in Formulation Example 1-5 were measured as follows.

After incubating each strain for 1 day, 200 µl was plated in a petri dish, 1 g of each extract was dissolved in 2 ml of 50% 1, 3-butylene glycol aqueous solution, and 30 µl of the mixed solution was taken. Each strain was inoculated on a paper disc and cultured under the following conditions (Table 12).

Table 12. Culture conditions of strains for measuring the antimicrobial effect of natural extracts according to the present invention

number Strains Medium Time, temperature type One Candida albicans (ATCC 10259) Nutrient agar 48 hours, 37 ℃ Exhalation 2 Escherichia coli (ATCC 25922) Tryticase soy agar 48 hours, 37 ℃ Exhalation 3 Streptococcus aureus (Staphylococcus aureus ATCC 29737) Nutrient agar 48 hours, 37 ℃ Exhalation 4 Bacillus subtilis ATCC 6633 Nutrient agar 48 hours, 37 ℃ Exhalation 5 Streptococcus mutans ATCC 25175 Brain heart infusion 24 hours, 37 ℃ Exhalation 6 Staphylococcus epidermidis ATCC 12228 Nutrient agar 48 hours, 27 ℃ Exhalation 7 Acne Bacteria (Propionibacterium acne ATCC 6919) Reinforced clostridial agar medium 72 hours, 37 ℃ anaerobe

The results are shown in Table 13 to measure the diameter of the clear zone appearing around the paper disc (paper disc).

Table 13. Experimental Results for Prescriptions Using Grapefruit Seed, Ginseng and Neem Fruit Extract

       (Clean area diameter unit: mm)

number sample Use strain (number of table 1) One 2 3 4 5 6 7 One Formulation Example 1 of Prescription Example 1 12 13 17 15 12 14 20 2 Comparative Formulation Example 2 of Formulation Example 1 0 0 0 0 0 0 0 3 Formulation Example 2 of Prescription Example 2 14 12 15 17 18 19 15 4 Comparative Formulation Example 2 of Prescription Example 2 0 0 0 0 0 0 0 5 Formulation Example 3 of Prescription Example 3 18 15 16 18 15 14 13 6 Comparative Formulation Example 3 of Prescription Example 3 0 0 0 0 0 0 0 7 Formulation Example 4 of Prescription Example 4 14 14 15 16 15 16 18 8 Comparative Formulation Example 4 of Prescription Example 4 0 0 0 0 0 0 0 9 Formulation Example 5 of Formulation Example 5 18 15 16 15 15 14 15 10 Comparative Formulation Example 5 of Prescription Example 5 0 0 0 0 0 0 0

Extracts obtained from grapefruit seeds, red ginseng, and nymph fruit of the present invention has a strong antibacterial action, the cosmetic composition of the present invention has the effect of treating or preventing skin diseases such as acne.

Claims (7)

A cosmetic composition for preventing acne containing a mixed extract obtained from grapefruit seeds, red ginseng and neem fruit. According to claim 1, wherein the grapefruit seeds, mixed extract obtained from the ginseng and Nim fruit, the completely dried grapefruit seed, alpine, Nim fruit is heated by extraction at 90-95 ℃ 4-20 hours, or 5-37 Acne-prevention cosmetic composition, characterized in that extracted by immersion at 1-15 days. According to claim 2, wherein the solvent used for the extraction is anti-acne cosmetic composition, characterized in that one or more solvents selected from the group consisting of water, ethanol, methanol, propanol, butanol, glycerin, propylene glycol, butylene glycol . The acetone of claim 2 wherein the solvent used for extraction is acetone. Ethyl acetate, benzene or chloroform cosmetic composition for preventing acne, characterized in that. The method of claim 2, wherein the solvent used for the extraction is anti-acne cosmetic composition, characterized in that the extract by adding a volume of 1-20 times the weight of the dried grapefruit seeds, red ginseng fruit. The cosmetic composition for preventing acne according to claim 1, which contains 0.001-10% (w / v) dry weight of grapefruit seed, Gosam and Nimbus fruit mixed extract. The cosmetic composition according to claim 1, wherein the cosmetic composition has a formulation such as softening cream (skin lotion), nutritious cream (milk lotion), nourishing cream, massage cream, essence, pack, emulsified foundation, or the like.